Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50071.t1 | XP_003606879.2 | 92.7 | 96 | 5 | 1 | 1 | 96 | 11 | 104 | 4.10E-40 | 173.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50071.t1 | G7JG36 | 92.7 | 96 | 5 | 1 | 1 | 96 | 1 | 94 | 3.0e-40 | 173.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049411 | MS.gene50071 | 0.825333 | 4.96E-54 | -1.69E-46 |
| MS.gene054004 | MS.gene50071 | 0.813463 | 2.53E-51 | -1.69E-46 |
| MS.gene055339 | MS.gene50071 | 0.832963 | 7.00E-56 | -1.69E-46 |
| MS.gene055765 | MS.gene50071 | 0.807822 | 4.19E-50 | -1.69E-46 |
| MS.gene056693 | MS.gene50071 | 0.99516 | 1.42E-213 | -1.69E-46 |
| MS.gene059802 | MS.gene50071 | 0.846679 | 1.87E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50071.t1 | MTR_4g069770 | 92.708 | 96 | 5 | 1 | 1 | 96 | 1 | 94 | 2.11e-61 | 182 |
| MS.gene50071.t1 | MTR_5g095980 | 60.215 | 93 | 30 | 2 | 1 | 87 | 1 | 92 | 1.28e-25 | 91.7 |
| MS.gene50071.t1 | MTR_3g007880 | 61.538 | 78 | 24 | 1 | 1 | 72 | 1 | 78 | 3.09e-22 | 83.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50071.t1 | AT1G49310 | 42.254 | 71 | 37 | 2 | 8 | 74 | 12 | 82 | 7.23e-13 | 58.9 |
Find 11 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTTTCTTCTTCTGCTTCT+TGG | 0.153872 | 4.2:+37086865 | None:intergenic |
| GAAAAGACTTGGGAGATTAT+TGG | 0.261061 | 4.2:-37087021 | MS.gene50071:CDS |
| ACAAGTTCTTTGATTGCTTC+AGG | 0.324229 | 4.2:+37086971 | None:intergenic |
| AACTCTGAACAAGAGCTTCA+TGG | 0.354626 | 4.2:-37086827 | MS.gene50071:CDS |
| CTCTTATGCAAGAAAAGACT+TGG | 0.442343 | 4.2:-37087032 | MS.gene50071:CDS |
| GAGTATGAGAGAAGCAAAGA+TGG | 0.451907 | 4.2:+37087217 | None:intergenic |
| TCTTTGATTGCTTCAGGCAT+AGG | 0.472850 | 4.2:+37086977 | None:intergenic |
| GTGTGATATAATATGACATT+AGG | 0.480243 | 4.2:+37086899 | None:intergenic |
| TGATTGCTTCAGGCATAGGT+TGG | 0.514568 | 4.2:+37086981 | None:intergenic |
| AGAACTTGTTCAAAATCAAG+AGG | 0.539159 | 4.2:-37086957 | MS.gene50071:CDS |
| TCTTATGCAAGAAAAGACTT+GGG | 0.611202 | 4.2:-37087031 | MS.gene50071:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAATATAGTATAAATTGAT+TGG | + | chr4.2:37086947-37086966 | None:intergenic | 15.0% |
| !!! | AATTTTCTTTGTACTAAAAT+AGG | - | chr4.2:37086979-37086998 | MS.gene50071:CDS | 15.0% |
| !! | TTAGTACAAAGAAAATTGAA+AGG | + | chr4.2:37086976-37086995 | None:intergenic | 20.0% |
| ! | GTGTGATATAATATGACATT+AGG | + | chr4.2:37087148-37087167 | None:intergenic | 25.0% |
| TCTTATGCAAGAAAAGACTT+GGG | - | chr4.2:37087013-37087032 | MS.gene50071:CDS | 30.0% | |
| ! | AGAACTTGTTCAAAATCAAG+AGG | - | chr4.2:37087087-37087106 | MS.gene50071:intron | 30.0% |
| ACAAGTTCTTTGATTGCTTC+AGG | + | chr4.2:37087076-37087095 | None:intergenic | 35.0% | |
| CTCTTATGCAAGAAAAGACT+TGG | - | chr4.2:37087012-37087031 | MS.gene50071:CDS | 35.0% | |
| GAAAAGACTTGGGAGATTAT+TGG | - | chr4.2:37087023-37087042 | MS.gene50071:CDS | 35.0% | |
| ! | AGTCTTTTCTTGCATAAGAG+AGG | + | chr4.2:37087012-37087031 | None:intergenic | 35.0% |
| ! | CATACTCTTTTCTCTTCTTC+TGG | - | chr4.2:37086843-37086862 | MS.gene50071:CDS | 35.0% |
| ! | TTGTTTCTTCTTCTGCTTCT+TGG | + | chr4.2:37087182-37087201 | None:intergenic | 35.0% |
| AACTCTGAACAAGAGCTTCA+TGG | - | chr4.2:37087217-37087236 | MS.gene50071:CDS | 40.0% | |
| GAGTATGAGAGAAGCAAAGA+TGG | + | chr4.2:37086830-37086849 | None:intergenic | 40.0% | |
| TCTTTGATTGCTTCAGGCAT+AGG | + | chr4.2:37087070-37087089 | None:intergenic | 40.0% | |
| TGATTGCTTCAGGCATAGGT+TGG | + | chr4.2:37087066-37087085 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 37086820 | 37087246 | 37086820 | ID=MS.gene50071 |
| chr4.2 | mRNA | 37086820 | 37087246 | 37086820 | ID=MS.gene50071.t1;Parent=MS.gene50071 |
| chr4.2 | exon | 37087202 | 37087246 | 37087202 | ID=MS.gene50071.t1.exon1;Parent=MS.gene50071.t1 |
| chr4.2 | CDS | 37087202 | 37087246 | 37087202 | ID=cds.MS.gene50071.t1;Parent=MS.gene50071.t1 |
| chr4.2 | exon | 37086820 | 37087065 | 37086820 | ID=MS.gene50071.t1.exon2;Parent=MS.gene50071.t1 |
| chr4.2 | CDS | 37086820 | 37087065 | 37086820 | ID=cds.MS.gene50071.t1;Parent=MS.gene50071.t1 |
| Gene Sequence |
| Protein sequence |
