Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50119.t1 | XP_003616495.1 | 97.8 | 273 | 6 | 0 | 1 | 273 | 1 | 273 | 2.40E-146 | 528.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50119.t1 | Q54TS4 | 34.3 | 198 | 120 | 3 | 77 | 269 | 125 | 317 | 5.9e-24 | 112.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50119.t1 | G7K200 | 97.8 | 273 | 6 | 0 | 1 | 273 | 1 | 273 | 1.8e-146 | 528.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene072464 | MS.gene50119 | PPI |
| MS.gene50119 | MS.gene050864 | PPI |
| MS.gene50119 | MS.gene29330 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50119.t1 | MTR_5g081040 | 97.802 | 273 | 6 | 0 | 1 | 273 | 1 | 273 | 0.0 | 544 |
| MS.gene50119.t1 | MTR_5g081040 | 97.619 | 210 | 5 | 0 | 64 | 273 | 4 | 213 | 6.23e-148 | 412 |
| MS.gene50119.t1 | MTR_3g069150 | 74.359 | 273 | 68 | 2 | 1 | 272 | 1 | 272 | 1.48e-141 | 399 |
| MS.gene50119.t1 | MTR_3g069170 | 73.750 | 240 | 62 | 1 | 1 | 239 | 1 | 240 | 1.17e-124 | 355 |
| MS.gene50119.t1 | MTR_3g069150 | 72.986 | 211 | 56 | 1 | 1 | 210 | 1 | 211 | 1.87e-107 | 312 |
| MS.gene50119.t1 | MTR_7g113620 | 37.945 | 253 | 146 | 4 | 1 | 246 | 2 | 250 | 1.33e-50 | 167 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50119.t1 | AT2G39805 | 60.932 | 279 | 97 | 5 | 1 | 272 | 1 | 274 | 4.31e-118 | 339 |
| MS.gene50119.t1 | AT2G39805 | 60.714 | 280 | 97 | 6 | 1 | 272 | 1 | 275 | 2.36e-116 | 335 |
| MS.gene50119.t1 | AT5G27490 | 38.434 | 281 | 160 | 6 | 1 | 272 | 2 | 278 | 9.19e-58 | 186 |
| MS.gene50119.t1 | AT3G05280 | 36.704 | 267 | 155 | 5 | 1 | 258 | 1 | 262 | 8.30e-52 | 171 |
Find 74 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGTAAGACCTCAAGTTTA+AGG | 0.239012 | 5.4:+63363409 | None:intergenic |
| ATGCTAACCTTATAAGGTTT+TGG | 0.250616 | 5.4:-63363795 | MS.gene50119:CDS |
| GTTCAAATGCTAACCTTATA+AGG | 0.256753 | 5.4:-63363801 | MS.gene50119:CDS |
| AATATTTGCATATTTGCTTC+AGG | 0.285108 | 5.4:+63369681 | None:intergenic |
| ACATGCACCAAAACCTTATA+AGG | 0.322668 | 5.4:+63363788 | None:intergenic |
| TACTGAAGAGATGATGAAAT+TGG | 0.329815 | 5.4:+63363750 | None:intergenic |
| ATTGTAGATACGGGCTTATA+TGG | 0.331360 | 5.4:-63364011 | MS.gene50119:intron |
| AGAAATAGAACGCACAAATC+AGG | 0.332070 | 5.4:+63364324 | None:intergenic |
| CTGAAGAAGTCTCCGCCAAC+TGG | 0.385581 | 5.4:+63364363 | None:intergenic |
| GTCTTTGTACTTGCCTCGCT+TGG | 0.388742 | 5.4:-63363973 | MS.gene50119:CDS |
| ACGGCTGCATGGTCTATCTA+TGG | 0.398905 | 5.4:-63363874 | MS.gene50119:CDS |
| CTACTTCTTCCTTCAGTACA+TGG | 0.404025 | 5.4:-63363824 | MS.gene50119:CDS |
| TGGCAGTCTTCATCAAGGTT+TGG | 0.405808 | 5.4:-63363331 | MS.gene50119:CDS |
| CTGTCTCCTCCGTTGGTGGC+GGG | 0.428585 | 5.4:+63369654 | None:intergenic |
| TGATGTTAGCTATATGAATA+CGG | 0.435253 | 5.4:-63363893 | MS.gene50119:CDS |
| TAAGAAGAAGATTCTTCCAT+TGG | 0.436573 | 5.4:+63370714 | None:intergenic |
| AACAACAGCCAACAAACAAC+TGG | 0.436583 | 5.4:-63364473 | MS.gene50119:CDS |
| TGGATCATAACACTCATTAC+TGG | 0.437777 | 5.4:-63363455 | MS.gene50119:CDS |
| ATTGAAGGAACTTATCAATC+TGG | 0.438429 | 5.4:+63364386 | None:intergenic |
| TCTGTCTCCTCCGTTGGTGG+CGG | 0.440294 | 5.4:+63369653 | None:intergenic |
| TCCTCTGTCTCCTCCGTTGG+TGG | 0.443214 | 5.4:+63369650 | None:intergenic |
| TATCTATGGTTATGCAATAG+TGG | 0.444016 | 5.4:-63363860 | MS.gene50119:CDS |
| ATATTTGCATATTTGCTTCA+GGG | 0.448587 | 5.4:+63369682 | None:intergenic |
| GTCTCCGCCAACTGGATTGA+AGG | 0.453964 | 5.4:+63364371 | None:intergenic |
| CAAACTGTTGGCACTCCTAC+TGG | 0.454589 | 5.4:-63369615 | MS.gene50119:intron |
| TGACATTTCAGTGGCGATCA+TGG | 0.461393 | 5.4:-63363378 | MS.gene50119:CDS |
| TATCTGCATGTTGTTGCATA+AGG | 0.465432 | 5.4:+63363935 | None:intergenic |
| ATCCACCGCAGAATCTCAAC+TGG | 0.468604 | 5.4:+63363473 | None:intergenic |
| CAACGGAGGAGACAGAGGAC+GGG | 0.471171 | 5.4:-63369646 | MS.gene50119:CDS |
| CCGTCCTCTGTCTCCTCCGT+TGG | 0.479438 | 5.4:+63369647 | None:intergenic |
| CTCCTCCGTTGGTGGCGGGA+GGG | 0.480975 | 5.4:+63369658 | None:intergenic |
| TCTCCTCCGTTGGTGGCGGG+AGG | 0.493774 | 5.4:+63369657 | None:intergenic |
| AACGGAGGAGACAGAGGACG+GGG | 0.501183 | 5.4:-63369645 | MS.gene50119:CDS |
| GCTTTGTTGCCTTAAACTTG+AGG | 0.501740 | 5.4:-63363418 | MS.gene50119:CDS |
| GAAATAGAACGCACAAATCA+GGG | 0.517354 | 5.4:+63364325 | None:intergenic |
| CAAGTTTAAGGCAACAAAGC+TGG | 0.520009 | 5.4:+63363421 | None:intergenic |
| AGGAAGAAGTAGTATGCCAT+TGG | 0.521354 | 5.4:+63363835 | None:intergenic |
| GAACCGAGCAAGTGGCTAGT+AGG | 0.527725 | 5.4:+63370681 | None:intergenic |
| CCAACGGAGGAGACAGAGGA+CGG | 0.529195 | 5.4:-63369647 | MS.gene50119:CDS |
| GTTCAGGCTGTCATAGTTGA+AGG | 0.541157 | 5.4:-63369843 | MS.gene50119:intron |
| CTTCCTACTAGCCACTTGCT+CGG | 0.543266 | 5.4:-63370684 | MS.gene50119:CDS |
| TACTTCTTCCTTCAGTACAT+GGG | 0.549442 | 5.4:-63363823 | MS.gene50119:CDS |
| GGAAGAAGTAGTATGCCATT+GGG | 0.550097 | 5.4:+63363836 | None:intergenic |
| ATAAGTTCCTTCAATCCAGT+TGG | 0.550727 | 5.4:-63364378 | MS.gene50119:CDS |
| AGTTCCTTCAATCCAGTTGG+CGG | 0.554962 | 5.4:-63364375 | MS.gene50119:CDS |
| ATGCAGATAGCGGTACCTCA+TGG | 0.555755 | 5.4:-63363921 | MS.gene50119:CDS |
| GAAGAATCTTCTTCTTACAC+TGG | 0.561265 | 5.4:-63370708 | MS.gene50119:CDS |
| AGGTCTGGCAGTCTTCATCA+AGG | 0.563444 | 5.4:-63363336 | MS.gene50119:CDS |
| CATTTGAACCCATGTACTGA+AGG | 0.576053 | 5.4:+63363815 | None:intergenic |
| CGGGGAGGATATCAAACTGT+TGG | 0.580950 | 5.4:-63369627 | MS.gene50119:CDS |
| GCTATATGAATACGGCTGCA+TGG | 0.592343 | 5.4:-63363885 | MS.gene50119:CDS |
| AACAGCCAACAAACAACTGG+AGG | 0.595623 | 5.4:-63364470 | MS.gene50119:CDS |
| ATATTCCCTCCCGCCACCAA+CGG | 0.595751 | 5.4:-63369663 | MS.gene50119:CDS |
| TATATGGATCTCAACGACAT+TGG | 0.600089 | 5.4:-63363995 | MS.gene50119:CDS |
| TTCCCTCCCGCCACCAACGG+AGG | 0.601298 | 5.4:-63369660 | MS.gene50119:CDS |
| CGGGAACTGAACCGAGCAAG+TGG | 0.601897 | 5.4:+63370673 | None:intergenic |
| GCCACCAACGGAGGAGACAG+AGG | 0.602397 | 5.4:-63369651 | MS.gene50119:CDS |
| GGAGGAGACAGAGGACGGGG+AGG | 0.603333 | 5.4:-63369642 | MS.gene50119:CDS |
| AAGAGATGATGAAATTGGAG+AGG | 0.607678 | 5.4:+63363755 | None:intergenic |
| AGAGATGATGAAATTGGAGA+GGG | 0.607964 | 5.4:+63363756 | None:intergenic |
| AGAAAAGACTACGAAACATG+TGG | 0.613192 | 5.4:-63369820 | MS.gene50119:CDS |
| AGCCAACAAACAACTGGAGG+GGG | 0.616047 | 5.4:-63364467 | MS.gene50119:CDS |
| ACTCATTACTGGTGTTGCGT+CGG | 0.617025 | 5.4:-63363444 | MS.gene50119:CDS |
| TAATTCCAGTTGAGATTCTG+CGG | 0.620402 | 5.4:-63363478 | MS.gene50119:CDS |
| TTATGCAATAGTGGTCCCAA+TGG | 0.635472 | 5.4:-63363851 | MS.gene50119:CDS |
| ACAGCCAACAAACAACTGGA+GGG | 0.635567 | 5.4:-63364469 | MS.gene50119:CDS |
| GAAAAGACTACGAAACATGT+GGG | 0.642609 | 5.4:-63369819 | MS.gene50119:intron |
| CAGCCAACAAACAACTGGAG+GGG | 0.648127 | 5.4:-63364468 | MS.gene50119:CDS |
| AGAAAGCAATGACATTTCAG+TGG | 0.681324 | 5.4:-63363387 | MS.gene50119:CDS |
| TTCCAGTTGAGATTCTGCGG+TGG | 0.686062 | 5.4:-63363475 | MS.gene50119:CDS |
| ATGCAACAACATGCAGATAG+CGG | 0.691113 | 5.4:-63363931 | MS.gene50119:CDS |
| TGTGGCAAGATTTCCAAGCG+AGG | 0.696815 | 5.4:+63363960 | None:intergenic |
| GCTAACATCAAAGCTCCATG+AGG | 0.728832 | 5.4:+63363906 | None:intergenic |
| ATGTTGTTGCATAAGGAATG+TGG | 0.728927 | 5.4:+63363942 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 63363319 | 63370733 | 63363319 | ID=MS.gene50119 |
| chr5.4 | mRNA | 63363319 | 63370733 | 63363319 | ID=MS.gene50119.t1;Parent=MS.gene50119 |
| chr5.4 | exon | 63370674 | 63370733 | 63370674 | ID=MS.gene50119.t1.exon1;Parent=MS.gene50119.t1 |
| chr5.4 | CDS | 63370674 | 63370733 | 63370674 | ID=cds.MS.gene50119.t1;Parent=MS.gene50119.t1 |
| chr5.4 | exon | 63369820 | 63369859 | 63369820 | ID=MS.gene50119.t1.exon2;Parent=MS.gene50119.t1 |
| chr5.4 | CDS | 63369820 | 63369859 | 63369820 | ID=cds.MS.gene50119.t1;Parent=MS.gene50119.t1 |
| chr5.4 | exon | 63369616 | 63369705 | 63369616 | ID=MS.gene50119.t1.exon3;Parent=MS.gene50119.t1 |
| chr5.4 | CDS | 63369616 | 63369705 | 63369616 | ID=cds.MS.gene50119.t1;Parent=MS.gene50119.t1 |
| chr5.4 | exon | 63364339 | 63364504 | 63364339 | ID=MS.gene50119.t1.exon4;Parent=MS.gene50119.t1 |
| chr5.4 | CDS | 63364339 | 63364504 | 63364339 | ID=cds.MS.gene50119.t1;Parent=MS.gene50119.t1 |
| chr5.4 | exon | 63363753 | 63364026 | 63363753 | ID=MS.gene50119.t1.exon5;Parent=MS.gene50119.t1 |
| chr5.4 | CDS | 63363753 | 63364026 | 63363753 | ID=cds.MS.gene50119.t1;Parent=MS.gene50119.t1 |
| chr5.4 | exon | 63363319 | 63363510 | 63363319 | ID=MS.gene50119.t1.exon6;Parent=MS.gene50119.t1 |
| chr5.4 | CDS | 63363319 | 63363510 | 63363319 | ID=cds.MS.gene50119.t1;Parent=MS.gene50119.t1 |
| Gene Sequence |
| Protein sequence |
