AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50341

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50341.t1 MTR_4g023030 97.271 623 17 0 1 623 1 623 0.0 1250
MS.gene50341.t1 MTR_3g026650 69.021 623 133 9 1 623 1 563 0.0 801
MS.gene50341.t1 MTR_3g026650 67.093 547 120 9 1 547 1 487 0.0 665
MS.gene50341.t1 MTR_3g026650 63.341 461 109 9 1 461 1 401 2.93e-177 508
MS.gene50341.t1 MTR_4g125460 36.744 430 252 8 198 616 58 478 1.13e-87 281
MS.gene50341.t1 MTR_4g125460 36.744 430 252 8 198 616 197 617 3.97e-86 281
MS.gene50341.t1 MTR_5g064620 36.261 444 237 10 202 616 85 511 8.96e-80 262
MS.gene50341.t1 MTR_3g096020 36.735 441 242 11 199 616 160 586 1.45e-79 263
MS.gene50341.t1 MTR_5g099010 36.427 431 255 9 196 619 84 502 2.69e-77 257
MS.gene50341.t1 MTR_4g100810 39.231 390 217 9 236 616 111 489 6.19e-77 254
MS.gene50341.t1 MTR_5g081690 34.967 449 271 10 179 616 72 510 2.69e-76 253
MS.gene50341.t1 MTR_3g115220 37.468 395 225 6 235 619 113 495 1.66e-75 252
MS.gene50341.t1 MTR_4g082310 36.409 401 236 8 236 622 100 495 6.93e-75 248
MS.gene50341.t1 MTR_3g099480 36.285 463 242 13 184 616 98 537 7.63e-75 249
MS.gene50341.t1 MTR_5g012770 37.907 430 234 11 202 622 95 500 8.52e-75 248
MS.gene50341.t1 MTR_4g019210 33.945 436 268 8 196 620 199 625 3.19e-73 248
MS.gene50341.t1 MTR_5g032100 34.581 454 246 10 202 616 60 501 1.59e-72 242
MS.gene50341.t1 MTR_5g042200 35.802 405 235 9 236 623 88 484 9.69e-71 237
MS.gene50341.t1 MTR_4g110030 37.634 372 220 6 236 598 119 487 1.27e-70 237
MS.gene50341.t1 MTR_6g016015 37.333 375 219 8 236 599 118 487 1.94e-70 237
MS.gene50341.t1 MTR_4g084570 34.014 441 255 11 204 622 127 553 2.43e-70 238
MS.gene50341.t1 MTR_1g031590 33.333 468 271 12 175 617 96 547 5.85e-70 236
MS.gene50341.t1 MTR_3g100310 34.305 446 240 10 211 616 83 515 1.66e-69 234
MS.gene50341.t1 MTR_2g059350 36.685 368 219 6 236 593 118 481 8.83e-69 233
MS.gene50341.t1 MTR_3g086570 32.883 444 261 12 199 616 115 547 5.16e-68 231
MS.gene50341.t1 MTR_5g059260 32.280 443 270 9 198 616 123 559 1.35e-64 223
MS.gene50341.t1 MTR_3g005340 33.333 396 248 6 236 616 150 544 6.35e-63 218
MS.gene50341.t1 MTR_1g102030 30.435 437 273 11 202 619 61 485 6.51e-58 203
MS.gene50341.t1 MTR_4g023050 95.000 80 4 0 544 623 29 108 8.01e-50 168
MS.gene50341.t1 MTR_2g016470 29.114 474 265 16 199 616 156 614 4.12e-49 181
MS.gene50341.t1 MTR_4g132590 31.130 469 261 17 199 616 35 492 6.09e-48 175
MS.gene50341.t1 MTR_4g132590 31.130 469 261 17 199 616 134 591 5.05e-47 175
MS.gene50341.t1 MTR_3g086570 31.077 325 190 10 199 498 115 430 1.26e-43 162
MS.gene50341.t1 MTR_1g101990 31.690 284 182 7 341 619 5 281 1.26e-42 156
MS.gene50341.t1 MTR_4g010990 27.068 399 235 13 236 616 78 438 1.17e-30 125
MS.gene50341.t1 MTR_7g017330 29.655 290 189 6 236 510 101 390 4.44e-30 126
MS.gene50341.t1 MTR_4g010990 26.425 386 228 13 249 616 2 349 5.31e-27 113
MS.gene50341.t1 MTR_3g092590 27.273 330 222 7 196 510 56 382 2.65e-26 114
MS.gene50341.t1 MTR_3g092590 27.273 330 222 7 196 510 56 382 3.50e-26 114
MS.gene50341.t1 MTR_1g088900 56.322 87 8 1 321 407 38 94 9.74e-23 98.2
MS.gene50341.t1 MTR_2g038320 24.603 378 220 13 213 569 77 410 6.39e-21 96.3
MS.gene50341.t1 MTR_4g008200 24.474 380 220 12 194 561 14 338 8.66e-21 95.1
MS.gene50341.t1 MTR_4g008200 24.093 386 214 12 194 561 65 389 1.42e-20 95.1
MS.gene50341.t1 MTR_1g007390 27.547 265 177 6 261 510 124 388 1.88e-20 96.3
MS.gene50341.t1 MTR_7g064210 31.100 209 129 4 339 535 19 224 2.19e-20 94.4
MS.gene50341.t1 MTR_1g101985 40.566 106 59 1 202 307 61 162 4.17e-17 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50341.t1 AT2G44500 63.235 612 156 8 11 618 17 563 0.0 751
MS.gene50341.t1 AT3G07900 59.806 617 190 12 10 621 14 577 0.0 685
MS.gene50341.t1 AT5G63390 56.930 469 191 3 156 623 97 555 0.0 555
MS.gene50341.t1 AT2G44500 56.264 455 130 8 11 461 17 406 3.92e-163 473
MS.gene50341.t1 AT5G35570 37.130 439 244 11 198 619 220 643 3.66e-81 269
MS.gene50341.t1 AT1G76270 35.909 440 249 10 194 619 69 489 6.66e-79 261
MS.gene50341.t1 AT1G20550 35.431 429 266 8 194 619 72 492 3.43e-78 259
MS.gene50341.t1 AT4G24530 35.590 458 242 11 199 616 72 516 2.65e-77 255
MS.gene50341.t1 AT5G64600 36.199 442 245 11 199 617 88 515 4.19e-76 252
MS.gene50341.t1 AT3G54100 33.879 428 267 7 202 620 195 615 7.94e-76 254
MS.gene50341.t1 AT1G22460 35.845 438 244 10 202 616 134 557 6.37e-75 250
MS.gene50341.t1 AT5G01100 34.101 434 266 8 202 623 190 615 6.91e-74 249
MS.gene50341.t1 AT2G37980 33.103 435 261 9 202 620 199 619 4.75e-73 247
MS.gene50341.t1 AT2G03280 38.070 373 215 7 237 598 92 459 5.66e-73 243
MS.gene50341.t1 AT4G38390 38.462 377 203 7 235 596 125 487 1.13e-72 244
MS.gene50341.t1 AT5G65470 33.696 460 252 10 199 617 56 503 1.27e-72 242
MS.gene50341.t1 AT1G14020 35.930 398 234 8 237 619 95 486 1.40e-69 234
MS.gene50341.t1 AT5G15740 36.193 373 224 6 236 598 109 477 3.19e-69 233
MS.gene50341.t1 AT1G14970 33.333 435 254 9 204 616 12 432 1.10e-68 230
MS.gene50341.t1 AT2G03280 35.500 400 215 8 237 598 92 486 2.54e-67 228
MS.gene50341.t1 AT4G16650 40.360 389 216 11 236 617 125 504 2.83e-67 229
MS.gene50341.t1 AT4G16650 40.360 389 216 11 236 617 125 504 2.83e-67 229
MS.gene50341.t1 AT1G14970 33.333 435 254 9 204 616 132 552 8.51e-67 228
MS.gene50341.t1 AT1G14970 33.738 412 245 7 226 616 2 406 1.41e-66 224
MS.gene50341.t1 AT3G02250 34.828 379 221 6 236 598 109 477 1.51e-65 224
MS.gene50341.t1 AT3G26370 33.668 398 244 8 236 616 161 555 5.14e-65 223
MS.gene50341.t1 AT1G04910 31.542 428 266 11 202 616 61 474 9.15e-65 222
MS.gene50341.t1 AT1G35510 32.118 439 262 9 202 618 134 558 1.31e-62 217
MS.gene50341.t1 AT1G38065 32.850 414 254 10 224 616 18 428 1.69e-62 214
MS.gene50341.t1 AT1G38065 33.085 402 246 9 236 616 39 438 1.77e-62 214
MS.gene50341.t1 AT2G01480 32.854 417 252 8 220 615 141 550 7.18e-62 215
MS.gene50341.t1 AT1G38131 32.850 414 254 10 224 616 147 557 1.96e-61 214
MS.gene50341.t1 AT2G01480 33.867 375 225 5 220 575 141 511 5.49e-60 209
MS.gene50341.t1 AT1G62330 29.193 483 259 15 198 618 190 651 2.65e-54 196
MS.gene50341.t1 AT1G11990 30.737 475 288 16 167 617 132 589 1.86e-53 192
MS.gene50341.t1 AT1G11990 29.106 481 267 15 198 617 144 611 1.58e-47 176
MS.gene50341.t1 AT1G29200 32.613 463 235 18 212 616 50 493 3.85e-47 173
MS.gene50341.t1 AT1G29200 32.613 463 235 18 212 616 50 493 3.85e-47 173
MS.gene50341.t1 AT1G29200 32.613 463 235 18 212 616 166 609 1.19e-46 174
MS.gene50341.t1 AT1G29200 33.333 432 222 16 238 616 1 419 1.30e-45 167
MS.gene50341.t1 AT3G30300 32.759 290 180 6 236 510 103 392 6.49e-35 140
MS.gene50341.t1 AT3G30300 32.759 290 180 6 236 510 103 392 6.49e-35 140
MS.gene50341.t1 AT1G52630 24.249 433 281 13 195 616 39 435 3.52e-29 121
MS.gene50341.t1 AT3G26370 32.967 182 108 4 236 404 161 341 8.14e-29 119
MS.gene50341.t1 AT3G03810 30.375 293 183 7 236 510 92 381 6.90e-27 116
MS.gene50341.t1 AT3G03810 30.375 293 183 7 236 510 92 381 6.90e-27 116
MS.gene50341.t1 AT1G52630 24.802 379 241 12 249 616 2 347 3.06e-25 107

Find 143 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTTATGGTAGTTGTTTC+TGG 0.133477 4.4:-78386460 MS.gene50341:CDS
ACCAAACCCATTTGAGATTA+AGG 0.207271 4.4:+78386867 None:intergenic
GCCATCTCATGGAGGAAATA+TGG 0.218103 4.4:-78384460 MS.gene50341:CDS
ATGACATTAACTTGCAAAAT+AGG 0.227744 4.4:+78386361 None:intergenic
GAGAGAAGAATTCAGAAAAT+AGG 0.237193 4.4:+78383899 None:intergenic
TCTTCACCACCTTAACAAAA+TGG 0.248210 4.4:-78387017 MS.gene50341:CDS
TATAGGTTGCTTAAAGCTTT+AGG 0.261962 4.4:-78384687 MS.gene50341:intron
GACCAGCCATCTTTCTTTCA+TGG 0.284935 4.4:+78385114 None:intergenic
GTTGTTATTGCAAGGATTCT+TGG 0.286345 4.4:-78386400 MS.gene50341:CDS
GTTTCCACATCTTTATAATA+AGG 0.294769 4.4:-78384583 MS.gene50341:CDS
TTTGCAAGTTAATGTCATTT+GGG 0.301248 4.4:-78386356 MS.gene50341:CDS
AAAAGATGCAAGAATTTATT+GGG 0.322642 4.4:-78384658 MS.gene50341:CDS
AAATATGGGCCATGCGCTTC+AGG 0.329180 4.4:-78384445 MS.gene50341:intron
GAAAAGTCCTTAATCTCAAA+TGG 0.329348 4.4:-78386874 MS.gene50341:CDS
AGAGAAGAATTCAGAAAATA+GGG 0.334594 4.4:+78383900 None:intergenic
AAGGATTCTTGGTGCTGCTT+TGG 0.336721 4.4:-78386389 MS.gene50341:CDS
CAAAACCCTTCAATCTCAAA+TGG 0.341182 4.4:-78386757 MS.gene50341:CDS
TCAAATTCACTTCCTTTGAT+TGG 0.342422 4.4:-78386664 MS.gene50341:CDS
CTCAGAAACTATGTAATCTA+TGG 0.350072 4.4:+78384500 None:intergenic
TGGAAGCAACCAGATGGGTT+AGG 0.362833 4.4:-78386556 MS.gene50341:CDS
TGACTTGGAGGAGGAAGAAA+AGG 0.368313 4.4:-78386605 MS.gene50341:CDS
ATGCTGTTAAACTCTGCTTC+AGG 0.374694 4.4:+78383876 None:intergenic
TCCTTAATCTCAAATGGGTT+TGG 0.376501 4.4:-78386868 MS.gene50341:CDS
AAAGGGTGAGGGAGTTGAAA+AGG 0.385869 4.4:-78386587 MS.gene50341:CDS
TTGAATAGAAGAAGATGAAA+TGG 0.388256 4.4:+78387098 None:intergenic
TGGTTCAAAATCTGAACTTA+AGG 0.388422 4.4:-78386848 MS.gene50341:CDS
GTTAGGGTATAAGCCTTGTT+TGG 0.390330 4.4:-78386539 MS.gene50341:CDS
GAATTGCATCAGAATTCATT+AGG 0.395674 4.4:-78383846 MS.gene50341:CDS
TGTGGAAACTCATGGATTAA+TGG 0.400222 4.4:+78384597 None:intergenic
GAATAGGAAAAGGTATCTTA+TGG 0.405053 4.4:-78386473 MS.gene50341:CDS
GCGAAGCCAGTTCAAGAACT+TGG 0.408751 4.4:-78385320 MS.gene50341:CDS
TTGTTTCCAAGTTCTTGAAC+TGG 0.412623 4.4:+78385314 None:intergenic
ACCTTGCTAGTCCTGAGTTC+CGG 0.418631 4.4:+78383819 None:intergenic
TCCGTTCGCGCTATCTCAAA+AGG 0.424692 4.4:-78385787 MS.gene50341:CDS
TGCGAAGAAAGCTTCTCTAA+TGG 0.432693 4.4:-78384526 MS.gene50341:CDS
CGAAACCACCCGCACATCAT+TGG 0.433723 4.4:+78385883 None:intergenic
GGAAGCAACCAGATGGGTTA+GGG 0.436626 4.4:-78386555 MS.gene50341:CDS
GAGTTGAAAAGGGTGAGTTT+TGG 0.437851 4.4:-78386576 MS.gene50341:CDS
TCAAACCCATTTGAGATTGA+AGG 0.439951 4.4:+78386751 None:intergenic
ATGCAAGAATTTATTGGGCC+GGG 0.443277 4.4:-78384653 MS.gene50341:CDS
AGAGAAGCTTTCTTCGCAAA+TGG 0.451160 4.4:+78384531 None:intergenic
GTATTTCTTGTGTCCTGCAT+AGG 0.451809 4.4:+78383950 None:intergenic
GGCTTATACCCTAACCCATC+TGG 0.457469 4.4:+78386547 None:intergenic
GTTATTCCTTCCAGGGTCAC+AGG 0.458415 4.4:-78383974 MS.gene50341:intron
TTTGTCATCAAGTGTGTTGA+TGG 0.461208 4.4:+78385854 None:intergenic
GTGACGGGATTGTGAAGAAT+AGG 0.461975 4.4:-78386489 MS.gene50341:CDS
GGTTCCTAGTGGAGTTGACT+TGG 0.467672 4.4:-78386620 MS.gene50341:CDS
TATAAAGATGTGGAAACTCA+TGG 0.469538 4.4:+78384589 None:intergenic
AAAAGTCCTTAATCTCAAAT+GGG 0.471123 4.4:-78386873 MS.gene50341:CDS
AAGGAAGATCTTGCATTGCC+TGG 0.471250 4.4:-78384564 MS.gene50341:CDS
GGAGGAGGAAGAAAAGGGTG+AGG 0.471319 4.4:-78386599 MS.gene50341:CDS
TCCACCCAATGGTTGTCCCC+CGG 0.472197 4.4:+78384635 None:intergenic
AAAACCCTTCAATCTCAAAT+GGG 0.474207 4.4:-78386756 MS.gene50341:CDS
AAGGGTGAGGGAGTTGAAAA+GGG 0.474419 4.4:-78386586 MS.gene50341:CDS
TGCTGTTAAACTCTGCTTCA+GGG 0.476049 4.4:+78383877 None:intergenic
CCATCTCATGGAGGAAATAT+GGG 0.480168 4.4:-78384459 MS.gene50341:CDS
TAGCGCGAACGGATCCAACT+TGG 0.483932 4.4:+78385797 None:intergenic
GTGAGGACTGGTTGTCTACC+CGG 0.488967 4.4:-78385221 MS.gene50341:CDS
CCTTGCTCTTCATCTAAGAA+TGG 0.490124 4.4:-78385258 MS.gene50341:CDS
GATGCAAGAATTTATTGGGC+CGG 0.492621 4.4:-78384654 MS.gene50341:CDS
AAGGATGTGTGGGTGAGGAC+TGG 0.493609 4.4:-78385233 MS.gene50341:CDS
ATCAAACTCAGGACTTAGAC+CGG 0.494512 4.4:+78385202 None:intergenic
CAAACCCATTTGAGATTGAA+GGG 0.494557 4.4:+78386752 None:intergenic
GATCTTCCTTTCAAGAGCTC+CGG 0.494862 4.4:+78385149 None:intergenic
CTCTTCATGTCACTCCAAGT+TGG 0.504620 4.4:-78385811 MS.gene50341:CDS
TGCAAGAATTTATTGGGCCG+GGG 0.505342 4.4:-78384652 MS.gene50341:CDS
GCAAATGGTTCTAGTTCTCC+AGG 0.505643 4.4:+78384546 None:intergenic
AACTCAGGACTAGCAAGGTC+GGG 0.506943 4.4:-78383815 MS.gene50341:CDS
AGCCTTGTTTGGATTTCAGT+AGG 0.508226 4.4:-78386528 MS.gene50341:CDS
TTGCACATGCACTCTGGGAC+AGG 0.510427 4.4:+78383774 None:intergenic
CCTTTGCAGTTCAACAGAGA+AGG 0.513180 4.4:-78385527 MS.gene50341:intron
TCTTTGTCCCTTGACTGCCA+TGG 0.516067 4.4:-78385093 MS.gene50341:CDS
TCCTAGTGGAGTTGACTTGG+AGG 0.524121 4.4:-78386617 MS.gene50341:CDS
ATTATAGAAGAGAAAGTGAC+GGG 0.525182 4.4:-78386504 MS.gene50341:CDS
ATGAGATTGTAAAGAATGAG+AGG 0.529082 4.4:-78385181 MS.gene50341:CDS
TCTTCATCTAAGAATGGAAA+AGG 0.529212 4.4:-78385252 MS.gene50341:CDS
GACTTGGAGGAGGAAGAAAA+GGG 0.531687 4.4:-78386604 MS.gene50341:CDS
ATGTCATTTGGGGAGATGAA+AGG 0.532142 4.4:-78386345 MS.gene50341:intron
GCCTTGTTTGGATTTCAGTA+GGG 0.532530 4.4:-78386527 MS.gene50341:CDS
GGTAACATCCATGGCAGTCA+AGG 0.535475 4.4:+78385085 None:intergenic
TCTTAGATGAAGAGCAAGGT+AGG 0.537764 4.4:+78385262 None:intergenic
AACATTACATTACCAATCAA+AGG 0.538428 4.4:+78386652 None:intergenic
GAATGAGAGGAGAGAACGAC+CGG 0.540471 4.4:-78385168 MS.gene50341:CDS
CGAAGCTTCTGAAGATCTGA+AGG 0.542834 4.4:+78385458 None:intergenic
TATTTACCTGGTAACATCCA+TGG 0.544732 4.4:+78385076 None:intergenic
CAGGGTCACAGGGCCTATGC+AGG 0.546366 4.4:-78383963 MS.gene50341:CDS
TCAGAATTCATTAGGACAGC+CGG 0.547453 4.4:-78383838 MS.gene50341:CDS
TGGAAAAGGATGTGTGGGTG+AGG 0.548660 4.4:-78385238 MS.gene50341:CDS
TAATGTAATGTTGCAAGCAA+AGG 0.548895 4.4:-78386641 MS.gene50341:CDS
GAGTCGTTGCACATGCACTC+TGG 0.553547 4.4:+78383768 None:intergenic
CATTAGGACAGCCGGAACTC+AGG 0.555136 4.4:-78383830 MS.gene50341:CDS
GAACGACCGGAGCTCTTGAA+AGG 0.557470 4.4:-78385155 MS.gene50341:CDS
TTTACAATCTCATCAAACTC+AGG 0.560411 4.4:+78385191 None:intergenic
GAGGAGGAAGAAAAGGGTGA+GGG 0.561708 4.4:-78386598 MS.gene50341:CDS
TACCATGAAAGAAAGATGGC+TGG 0.562902 4.4:-78385116 MS.gene50341:CDS
CCCATATTTCCTCCATGAGA+TGG 0.566943 4.4:+78384459 None:intergenic
ATGGAATTGATTATTTGTGA+AGG 0.568945 4.4:+78387117 None:intergenic
TCTTCAGAAGCTTCGATGCA+AGG 0.571820 4.4:-78385450 MS.gene50341:intron
AGAGGAGGACTTCCCTCCAC+AGG 0.571987 4.4:+78385830 None:intergenic
CAACTTGGAGTGACATGAAG+AGG 0.572279 4.4:+78385812 None:intergenic
TAGTGGAGTTGACTTGGAGG+AGG 0.574980 4.4:-78386614 MS.gene50341:CDS
GATTATAGAAGAGAAAGTGA+CGG 0.575059 4.4:-78386505 MS.gene50341:CDS
AGTCGTTGCACATGCACTCT+GGG 0.575723 4.4:+78383769 None:intergenic
AAAAGAAGAACAATCTTGAA+GGG 0.576810 4.4:+78386989 None:intergenic
CCGTTCGCGCTATCTCAAAA+GGG 0.577744 4.4:-78385786 MS.gene50341:intron
GACTTACCATGAAAGAAAGA+TGG 0.579868 4.4:-78385120 MS.gene50341:CDS
GAACTCAGGACTAGCAAGGT+CGG 0.582708 4.4:-78383816 MS.gene50341:CDS
TTATTCCTTCCAGGGTCACA+GGG 0.583835 4.4:-78383973 MS.gene50341:intron
GGATTGTGAAGAATAGGAAA+AGG 0.584135 4.4:-78386483 MS.gene50341:CDS
TTTGATTCAAAATCTGAACT+TGG 0.584212 4.4:-78386733 MS.gene50341:CDS
TGTTCTTGCCAATGATGTGC+GGG 0.584306 4.4:-78385891 MS.gene50341:CDS
TTCAACATTTGTAGCACATG+GGG 0.585771 4.4:+78386894 None:intergenic
CTTGGATTCAAGGCTATCAA+AGG 0.589401 4.4:-78385486 MS.gene50341:CDS
TCTTCCTTATTATAAAGATG+TGG 0.591443 4.4:+78384579 None:intergenic
TGACTGCCATGGATGTTACC+AGG 0.594053 4.4:-78385082 MS.gene50341:intron
TTGATGCTGTTGTTATTGCA+AGG 0.595939 4.4:-78386408 MS.gene50341:CDS
TCCTCCAAGTCAACTCCACT+AGG 0.596678 4.4:+78386616 None:intergenic
GCAAGAATTTATTGGGCCGG+GGG 0.602690 4.4:-78384651 MS.gene50341:CDS
CTTATGGTAGTTGTTTCTGG+AGG 0.604646 4.4:-78386457 MS.gene50341:CDS
TTGCAAGTTAATGTCATTTG+GGG 0.605474 4.4:-78386355 MS.gene50341:CDS
TCAGGACTAGCAAGGTCGGG+AGG 0.608590 4.4:-78383812 MS.gene50341:CDS
CTTGATGACAAAGCCTGTGG+AGG 0.609422 4.4:-78385843 MS.gene50341:CDS
CTTAGATGAAGAGCAAGGTA+GGG 0.612955 4.4:+78385263 None:intergenic
TTTCAACATTTGTAGCACAT+GGG 0.614324 4.4:+78386893 None:intergenic
CCATTCTTAGATGAAGAGCA+AGG 0.618532 4.4:+78385258 None:intergenic
GTAACATCCATGGCAGTCAA+GGG 0.621567 4.4:+78385086 None:intergenic
TTGATGACAAAGCCTGTGGA+GGG 0.624103 4.4:-78385842 MS.gene50341:CDS
GCTGAGAGAATGCAAAGCAA+AGG 0.624230 4.4:-78385287 MS.gene50341:CDS
AAGAATGGAAAAGGATGTGT+GGG 0.627477 4.4:-78385243 MS.gene50341:CDS
CAGGACTAGCAAGGTCGGGA+GGG 0.628531 4.4:-78383811 MS.gene50341:CDS
TGTTTCAATGAATAAAGATG+AGG 0.632505 4.4:-78386707 MS.gene50341:CDS
TTTGGTAAAATCTGAACTTG+GGG 0.642353 4.4:-78386785 MS.gene50341:CDS
CTTGGAGTGACATGAAGAGG+AGG 0.650220 4.4:+78385815 None:intergenic
TCCCTACTGAAATCCAAACA+AGG 0.655365 4.4:+78386526 None:intergenic
TTGCAAGCAAAGGTTCCTAG+TGG 0.655758 4.4:-78386631 MS.gene50341:CDS
TAAGAATGGAAAAGGATGTG+TGG 0.664578 4.4:-78385244 MS.gene50341:CDS
GCCGGAACTCAGGACTAGCA+AGG 0.665088 4.4:-78383820 MS.gene50341:CDS
CTGGAGGAATGAATCAGCAG+AGG 0.666148 4.4:-78386441 MS.gene50341:CDS
ATAGTCATACCTGAAGCGCA+TGG 0.668438 4.4:+78384436 None:intergenic
TCAAACTCAGGACTTAGACC+GGG 0.669861 4.4:+78385203 None:intergenic
ACACTTGATGACAAAGCCTG+TGG 0.670917 4.4:-78385846 MS.gene50341:CDS
ATGTTCTTGCCAATGATGTG+CGG 0.671242 4.4:-78385892 MS.gene50341:CDS
TCTTGCCAATGATGTGCGGG+TGG 0.696204 4.4:-78385888 MS.gene50341:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTATATTTTTTTCTCAT+TGG - chr4.4:78384945-78384964 MS.gene50341:intron 10.0%
!!! TTTATTTGTTTTTGATTTAT+AGG - chr4.4:78386213-78386232 MS.gene50341:intron 10.0%
!! TTATTAAATATGAAGACTAA+AGG + chr4.4:78386689-78386708 None:intergenic 15.0%
!!! AATAAAGATGAGATTTTTAA+TGG - chr4.4:78384103-78384122 MS.gene50341:intron 15.0%
!!! ATGATACAATCAATTATTTT+AGG - chr4.4:78386742-78386761 MS.gene50341:CDS 15.0%
!!! TGATACAATCAATTATTTTA+GGG - chr4.4:78386743-78386762 MS.gene50341:CDS 15.0%
!! AAAAAGATGCAAGAATTTAT+TGG - chr4.4:78386258-78386277 MS.gene50341:intron 20.0%
!! AAAAGATGCAAGAATTTATT+GGG - chr4.4:78386259-78386278 MS.gene50341:intron 20.0%
!! AATCAATGAAGATTTATGTA+AGG - chr4.4:78384649-78384668 MS.gene50341:CDS 20.0%
!! AATTGCATGATTACAATTTA+TGG + chr4.4:78386813-78386832 None:intergenic 20.0%
!! AGAAGAATTTAATATTTACC+TGG + chr4.4:78385856-78385875 None:intergenic 20.0%
!! ATATTTAGCTGTTACAAATT+TGG - chr4.4:78384759-78384778 MS.gene50341:intron 20.0%
!! GCTAAATATATATCCAAATT+TGG + chr4.4:78384748-78384767 None:intergenic 20.0%
!! TTACACTTATGAAACTATTT+GGG - chr4.4:78385929-78385948 MS.gene50341:CDS 20.0%
!! TTCTGTGGAAATAAAAAATT+GGG - chr4.4:78385323-78385342 MS.gene50341:CDS 20.0%
!! TTTACACTTATGAAACTATT+TGG - chr4.4:78385928-78385947 MS.gene50341:CDS 20.0%
!!! AAATTCTTCTACTTTTGTAT+AGG - chr4.4:78385866-78385885 MS.gene50341:CDS 20.0%
!!! AGGTTTAGTTATTACAATTT+TGG - chr4.4:78384669-78384688 MS.gene50341:CDS 20.0%
!!! ATTTTTGATGAAGATTTCTT+TGG + chr4.4:78383866-78383885 None:intergenic 20.0%
!!! TATTTTTTTCTCATTGGTTT+TGG - chr4.4:78384951-78384970 MS.gene50341:intron 20.0%
!!! TGTGCTTTTAGTAATTAAAA+AGG - chr4.4:78386715-78386734 MS.gene50341:CDS 20.0%
!!! TTATGGAGAAAATATGTTTT+TGG + chr4.4:78386796-78386815 None:intergenic 20.0%
! AAAAAGAAGAACAATCTTGA+AGG + chr4.4:78383932-78383951 None:intergenic 25.0%
! AAAAGAAGAACAATCTTGAA+GGG + chr4.4:78383931-78383950 None:intergenic 25.0%
! AAAAGTCCTTAATCTCAAAT+GGG - chr4.4:78384044-78384063 MS.gene50341:intron 25.0%
! AACATTACATTACCAATCAA+AGG + chr4.4:78384268-78384287 None:intergenic 25.0%
! AATTAAGATAGTCACATTGA+TGG - chr4.4:78386829-78386848 MS.gene50341:CDS 25.0%
! AGAGAAGAATTCAGAAAATA+GGG + chr4.4:78387020-78387039 None:intergenic 25.0%
! ATACAGTATATATGAATGCA+TGG + chr4.4:78386601-78386620 None:intergenic 25.0%
! ATGGAATTGATTATTTGTGA+AGG + chr4.4:78383803-78383822 None:intergenic 25.0%
! ATTGTTTGAAAGACCAAATT+TGG - chr4.4:78384732-78384751 MS.gene50341:intron 25.0%
! CTTCTGTGGAAATAAAAAAT+TGG - chr4.4:78385322-78385341 MS.gene50341:CDS 25.0%
! GTAATCATGCTTACATATAT+TGG - chr4.4:78386575-78386594 MS.gene50341:CDS 25.0%
! GTTTCCACATCTTTATAATA+AGG - chr4.4:78386334-78386353 MS.gene50341:intron 25.0%
! TCTTCCTTATTATAAAGATG+TGG + chr4.4:78386341-78386360 None:intergenic 25.0%
! TGTTTCAATGAATAAAGATG+AGG - chr4.4:78384093-78384112 MS.gene50341:intron 25.0%
! TTGAATAGAAGAAGATGAAA+TGG + chr4.4:78383822-78383841 None:intergenic 25.0%
! TTTACTATTCTTCTTGTTCA+TGG - chr4.4:78384600-78384619 MS.gene50341:CDS 25.0%
! TTTGATTCAAAATCTGAACT+TGG - chr4.4:78384184-78384203 MS.gene50341:intron 25.0%
!! AAGGTGAGTACAAATTTTAT+AGG + chr4.4:78386670-78386689 None:intergenic 25.0%
!! AATCTTTTTGTTCTTGATAC+TGG - chr4.4:78384851-78384870 MS.gene50341:intron 25.0%
!! AGGTGAGTACAAATTTTATA+GGG + chr4.4:78386669-78386688 None:intergenic 25.0%
!! TTTGCAAGTTAATGTCATTT+GGG - chr4.4:78384561-78384580 MS.gene50341:CDS 25.0%
!! TTTTGCAAGTTAATGTCATT+TGG - chr4.4:78384560-78384579 MS.gene50341:CDS 25.0%
!! TTTTGGTAAAATCTGAACTT+GGG - chr4.4:78384131-78384150 MS.gene50341:intron 25.0%
!! TTTTTCACACTCTTTTTTCT+TGG - chr4.4:78383947-78383966 MS.gene50341:CDS 25.0%
!!! AATTATTTTAGGGATGTTGA+TGG - chr4.4:78386753-78386772 MS.gene50341:CDS 25.0%
!!! ATGACATTAACTTGCAAAAT+AGG + chr4.4:78384559-78384578 None:intergenic 25.0%
!!! ATTATTTTAGGGATGTTGAT+GGG - chr4.4:78386754-78386773 MS.gene50341:CDS 25.0%
!!! GATTTTTAATGGTGATGTTT+TGG - chr4.4:78384114-78384133 MS.gene50341:intron 25.0%
AAAACCCTTCAATCTCAAAT+GGG - chr4.4:78384161-78384180 MS.gene50341:intron 30.0%
AATAAGCTGGAAACAGTTTA+TGG + chr4.4:78385981-78386000 None:intergenic 30.0%
ATCTTCATCTGTGTAACAAT+CGG - chr4.4:78385510-78385529 MS.gene50341:CDS 30.0%
ATGAGATTGTAAAGAATGAG+AGG - chr4.4:78385736-78385755 MS.gene50341:intron 30.0%
ATTATAGAAGAGAAAGTGAC+GGG - chr4.4:78384413-78384432 MS.gene50341:intron 30.0%
CAAAAACTTAGCAACAACTT+AGG + chr4.4:78385206-78385225 None:intergenic 30.0%
CTCAGAAACTATGTAATCTA+TGG + chr4.4:78386420-78386439 None:intergenic 30.0%
GAAAAGTCCTTAATCTCAAA+TGG - chr4.4:78384043-78384062 MS.gene50341:intron 30.0%
GAATTGCATCAGAATTCATT+AGG - chr4.4:78387071-78387090 MS.gene50341:CDS 30.0%
GAGAGAAGAATTCAGAAAAT+AGG + chr4.4:78387021-78387040 None:intergenic 30.0%
GATTATAGAAGAGAAAGTGA+CGG - chr4.4:78384412-78384431 MS.gene50341:intron 30.0%
TAAAAGCAACCTGAAAACAT+AGG + chr4.4:78385563-78385582 None:intergenic 30.0%
TAACACACTTTCTCATCAAA+GGG + chr4.4:78385266-78385285 None:intergenic 30.0%
TAATGTAATGTTGCAAGCAA+AGG - chr4.4:78384276-78384295 MS.gene50341:intron 30.0%
TATAAAGATGTGGAAACTCA+TGG + chr4.4:78386331-78386350 None:intergenic 30.0%
TATAGGTTGCTTAAAGCTTT+AGG - chr4.4:78386230-78386249 MS.gene50341:intron 30.0%
TCAAATTCACTTCCTTTGAT+TGG - chr4.4:78384253-78384272 MS.gene50341:intron 30.0%
TCTTCATCTAAGAATGGAAA+AGG - chr4.4:78385665-78385684 MS.gene50341:intron 30.0%
TCTTCTAGCATTCACAAATA+AGG + chr4.4:78384911-78384930 None:intergenic 30.0%
TGGTTCAAAATCTGAACTTA+AGG - chr4.4:78384069-78384088 MS.gene50341:intron 30.0%
TGTTTATCCAAAGATCCTAT+AGG - chr4.4:78386144-78386163 MS.gene50341:intron 30.0%
TTAACACACTTTCTCATCAA+AGG + chr4.4:78385267-78385286 None:intergenic 30.0%
TTATAGGGACTAAAGTTGAA+AGG + chr4.4:78386654-78386673 None:intergenic 30.0%
TTTACAATCTCATCAAACTC+AGG + chr4.4:78385729-78385748 None:intergenic 30.0%
TTTCAACATTTGTAGCACAT+GGG + chr4.4:78384027-78384046 None:intergenic 30.0%
TTTGGTAAAATCTGAACTTG+GGG - chr4.4:78384132-78384151 MS.gene50341:intron 30.0%
! ACTGATTTCATAAGCTATCT+TGG + chr4.4:78386016-78386035 None:intergenic 30.0%
! AGGGTTTTTCCATTTTGTTA+AGG + chr4.4:78383912-78383931 None:intergenic 30.0%
! CTCTTATTCGAGTTTATTAC+TGG - chr4.4:78385903-78385922 MS.gene50341:CDS 30.0%
! GTTTTTCCATTTTGTTAAGG+TGG + chr4.4:78383909-78383928 None:intergenic 30.0%
! TATCTTATGGTAGTTGTTTC+TGG - chr4.4:78384457-78384476 MS.gene50341:CDS 30.0%
! TTGCAAGTTAATGTCATTTG+GGG - chr4.4:78384562-78384581 MS.gene50341:CDS 30.0%
! TTTTCAACATTTGTAGCACA+TGG + chr4.4:78384028-78384047 None:intergenic 30.0%
!! ACTCATGTTTTTGCATCTTT+AGG - chr4.4:78385173-78385192 MS.gene50341:CDS 30.0%
!! ATGCATTTGTCTTTTGTTTG+GGG + chr4.4:78386997-78387016 None:intergenic 30.0%
!! GAATAGGAAAAGGTATCTTA+TGG - chr4.4:78384444-78384463 MS.gene50341:intron 30.0%
!! GATGCATTTGTCTTTTGTTT+GGG + chr4.4:78386998-78387017 None:intergenic 30.0%
!! GTTTTGGTAAAATCTGAACT+TGG - chr4.4:78384130-78384149 MS.gene50341:intron 30.0%
!!! GGTTTTGCTTTGTTTATGAT+TGG - chr4.4:78383971-78383990 MS.gene50341:intron 30.0%
AAACTATTTGGGAGAGCTTA+TGG - chr4.4:78385940-78385959 MS.gene50341:intron 35.0%
AACAAGATCAATCTATGCCT+AGG + chr4.4:78384707-78384726 None:intergenic 35.0%
AAGAATGGAAAAGGATGTGT+GGG - chr4.4:78385674-78385693 MS.gene50341:intron 35.0%
ACCTAAATGTTATTCCTTCC+AGG - chr4.4:78386935-78386954 MS.gene50341:CDS 35.0%
CAAAACCCTTCAATCTCAAA+TGG - chr4.4:78384160-78384179 MS.gene50341:intron 35.0%
CCTAAATGTTATTCCTTCCA+GGG - chr4.4:78386936-78386955 MS.gene50341:CDS 35.0%
GACTTACCATGAAAGAAAGA+TGG - chr4.4:78385797-78385816 MS.gene50341:CDS 35.0%
GGATTGTGAAGAATAGGAAA+AGG - chr4.4:78384434-78384453 MS.gene50341:intron 35.0%
TAAGAATGGAAAAGGATGTG+TGG - chr4.4:78385673-78385692 MS.gene50341:intron 35.0%
TATTTACCTGGTAACATCCA+TGG + chr4.4:78385844-78385863 None:intergenic 35.0%
TCTTCACCACCTTAACAAAA+TGG - chr4.4:78383900-78383919 MS.gene50341:CDS 35.0%
TGTTGAGCTTGAGTTTAACA+TGG - chr4.4:78385357-78385376 MS.gene50341:intron 35.0%
TTCAACATTTGTAGCACATG+GGG + chr4.4:78384026-78384045 None:intergenic 35.0%
TTGTTTCCAAGTTCTTGAAC+TGG + chr4.4:78385606-78385625 None:intergenic 35.0%
TTTGGACTTAATCTAAGCCT+AGG - chr4.4:78384687-78384706 MS.gene50341:intron 35.0%
TTTGTCATCAAGTGTGTTGA+TGG + chr4.4:78385066-78385085 None:intergenic 35.0%
! AATGAAAGCTTTGACTTCTG+TGG - chr4.4:78385308-78385327 MS.gene50341:CDS 35.0%
! ACCAAACCCATTTGAGATTA+AGG + chr4.4:78384053-78384072 None:intergenic 35.0%
! CAAACCCATTTGAGATTGAA+GGG + chr4.4:78384168-78384187 None:intergenic 35.0%
! CTTTTGTATAGGAGTCTGTT+TGG - chr4.4:78385877-78385896 MS.gene50341:CDS 35.0%
! GGATGCATTTGTCTTTTGTT+TGG + chr4.4:78386999-78387018 None:intergenic 35.0%
! GTTGTTATTGCAAGGATTCT+TGG - chr4.4:78384517-78384536 MS.gene50341:CDS 35.0%
! TCAAACCCATTTGAGATTGA+AGG + chr4.4:78384169-78384188 None:intergenic 35.0%
! TCCTTAATCTCAAATGGGTT+TGG - chr4.4:78384049-78384068 MS.gene50341:intron 35.0%
! TGTGGAAACTCATGGATTAA+TGG + chr4.4:78386323-78386342 None:intergenic 35.0%
! TTGATGCTGTTGTTATTGCA+AGG - chr4.4:78384509-78384528 MS.gene50341:CDS 35.0%
!! TTGATGGGTCGTGATTTTAT+AGG - chr4.4:78386769-78386788 MS.gene50341:CDS 35.0%
!!! GCACATGGGGTTTTAAAAAA+CGG + chr4.4:78384013-78384032 None:intergenic 35.0%
!!! TTCACACTCTTTTTTCTTGG+TGG - chr4.4:78383950-78383969 MS.gene50341:CDS 35.0%
AAGCTATCTTGGAGATCTTG+TGG + chr4.4:78386005-78386024 None:intergenic 40.0%
AGAGAAGCTTTCTTCGCAAA+TGG + chr4.4:78386389-78386408 None:intergenic 40.0%
AGCTATCTTGGAGATCTTGT+GGG + chr4.4:78386004-78386023 None:intergenic 40.0%
AGTGAAGCCTATAGGATCTT+TGG + chr4.4:78386154-78386173 None:intergenic 40.0%
ATCAAACTCAGGACTTAGAC+CGG + chr4.4:78385718-78385737 None:intergenic 40.0%
ATGCAAGAATTTATTGGGCC+GGG - chr4.4:78386264-78386283 MS.gene50341:intron 40.0%
ATGCTGTTAAACTCTGCTTC+AGG + chr4.4:78387044-78387063 None:intergenic 40.0%
ATGTTCTTGCCAATGATGTG+CGG - chr4.4:78385025-78385044 MS.gene50341:intron 40.0%
CACTGAAAAGTGAAGCCTAT+AGG + chr4.4:78386162-78386181 None:intergenic 40.0%
CCATCTCATGGAGGAAATAT+GGG - chr4.4:78386458-78386477 MS.gene50341:CDS 40.0%
CCATTCTTAGATGAAGAGCA+AGG + chr4.4:78385662-78385681 None:intergenic 40.0%
CCCTGGAAGGAATAACATTT+AGG + chr4.4:78386939-78386958 None:intergenic 40.0%
CCTTGCTCTTCATCTAAGAA+TGG - chr4.4:78385659-78385678 MS.gene50341:intron 40.0%
CTTAGATGAAGAGCAAGGTA+GGG + chr4.4:78385657-78385676 None:intergenic 40.0%
CTTGGATTCAAGGCTATCAA+AGG - chr4.4:78385431-78385450 MS.gene50341:intron 40.0%
GATGCAAGAATTTATTGGGC+CGG - chr4.4:78386263-78386282 MS.gene50341:intron 40.0%
GTATTTCTTGTGTCCTGCAT+AGG + chr4.4:78386970-78386989 None:intergenic 40.0%
TACCATGAAAGAAAGATGGC+TGG - chr4.4:78385801-78385820 MS.gene50341:CDS 40.0%
TCAGAATTCATTAGGACAGC+CGG - chr4.4:78387079-78387098 MS.gene50341:CDS 40.0%
TCCCTACTGAAATCCAAACA+AGG + chr4.4:78384394-78384413 None:intergenic 40.0%
TCTTAGATGAAGAGCAAGGT+AGG + chr4.4:78385658-78385677 None:intergenic 40.0%
TGCGAAGAAAGCTTCTCTAA+TGG - chr4.4:78386391-78386410 MS.gene50341:CDS 40.0%
TGCTGTTAAACTCTGCTTCA+GGG + chr4.4:78387043-78387062 None:intergenic 40.0%
! AAAGCTTCTTTTCCACCCAA+TGG + chr4.4:78386296-78386315 None:intergenic 40.0%
! ATGTCATTTGGGGAGATGAA+AGG - chr4.4:78384572-78384591 MS.gene50341:CDS 40.0%
! GAGTTGAAAAGGGTGAGTTT+TGG - chr4.4:78384341-78384360 MS.gene50341:intron 40.0%
! GATGTTTTCATGCCATCTCA+TGG - chr4.4:78386446-78386465 MS.gene50341:CDS 40.0%
! TTTTACGCAGCTTGGATTCA+AGG - chr4.4:78385421-78385440 MS.gene50341:intron 40.0%
!! AGCCTTGTTTGGATTTCAGT+AGG - chr4.4:78384389-78384408 MS.gene50341:intron 40.0%
!! CTATAGGCTTCACTTTTCAG+TGG - chr4.4:78386160-78386179 MS.gene50341:intron 40.0%
!! CTTATGGTAGTTGTTTCTGG+AGG - chr4.4:78384460-78384479 MS.gene50341:CDS 40.0%
!! GCCTTGTTTGGATTTCAGTA+GGG - chr4.4:78384390-78384409 MS.gene50341:intron 40.0%
!! GTTAGGGTATAAGCCTTGTT+TGG - chr4.4:78384378-78384397 MS.gene50341:intron 40.0%
!! TGTTTTGCTTTTACGCAGCT+TGG - chr4.4:78385413-78385432 MS.gene50341:intron 40.0%
!!! AGTTTTGGAAGCAACCAGAT+GGG - chr4.4:78384356-78384375 MS.gene50341:intron 40.0%
AAAGGGTGAGGGAGTTGAAA+AGG - chr4.4:78384330-78384349 MS.gene50341:intron 45.0%
AAGGAAGATCTTGCATTGCC+TGG - chr4.4:78386353-78386372 MS.gene50341:CDS 45.0%
AAGGGTGAGGGAGTTGAAAA+GGG - chr4.4:78384331-78384350 MS.gene50341:intron 45.0%
ATAGTCATACCTGAAGCGCA+TGG + chr4.4:78386484-78386503 None:intergenic 45.0%
CAACTTGGAGTGACATGAAG+AGG + chr4.4:78385108-78385127 None:intergenic 45.0%
CCCATATTTCCTCCATGAGA+TGG + chr4.4:78386461-78386480 None:intergenic 45.0%
CCTTCTCTGTTGAACTGCAA+AGG + chr4.4:78385393-78385412 None:intergenic 45.0%
CCTTTGCAGTTCAACAGAGA+AGG - chr4.4:78385390-78385409 MS.gene50341:intron 45.0%
CTCTTCATGTCACTCCAAGT+TGG - chr4.4:78385106-78385125 MS.gene50341:CDS 45.0%
GACCAGCCATCTTTCTTTCA+TGG + chr4.4:78385806-78385825 None:intergenic 45.0%
GACTTGGAGGAGGAAGAAAA+GGG - chr4.4:78384313-78384332 MS.gene50341:intron 45.0%
GAGATCTTGTGGGAATAAGC+TGG + chr4.4:78385994-78386013 None:intergenic 45.0%
GATCTTCCTTTCAAGAGCTC+CGG + chr4.4:78385771-78385790 None:intergenic 45.0%
GCCATCTCATGGAGGAAATA+TGG - chr4.4:78386457-78386476 MS.gene50341:CDS 45.0%
GCTGAGAGAATGCAAAGCAA+AGG - chr4.4:78385630-78385649 MS.gene50341:intron 45.0%
GTAACATCCATGGCAGTCAA+GGG + chr4.4:78385834-78385853 None:intergenic 45.0%
TCAAACTCAGGACTTAGACC+GGG + chr4.4:78385717-78385736 None:intergenic 45.0%
TCTTCAGAAGCTTCGATGCA+AGG - chr4.4:78385467-78385486 MS.gene50341:CDS 45.0%
TGCAAGAATTTATTGGGCCG+GGG - chr4.4:78386265-78386284 MS.gene50341:intron 45.0%
TGTTCTTGCCAATGATGTGC+GGG - chr4.4:78385026-78385045 MS.gene50341:intron 45.0%
TTATTCCTTCCAGGGTCACA+GGG - chr4.4:78386944-78386963 MS.gene50341:CDS 45.0%
TTGCAAGCAAAGGTTCCTAG+TGG - chr4.4:78384286-78384305 MS.gene50341:intron 45.0%
! ACACTTGATGACAAAGCCTG+TGG - chr4.4:78385071-78385090 MS.gene50341:intron 45.0%
! CGAAGCTTCTGAAGATCTGA+AGG + chr4.4:78385462-78385481 None:intergenic 45.0%
! CTCTGCTCACCTATGTTTTC+AGG - chr4.4:78385551-78385570 MS.gene50341:intron 45.0%
! GTGACGGGATTGTGAAGAAT+AGG - chr4.4:78384428-78384447 MS.gene50341:intron 45.0%
! GTTTTCATGCCATCTCATGG+AGG - chr4.4:78386449-78386468 MS.gene50341:CDS 45.0%
! TGACTTGGAGGAGGAAGAAA+AGG - chr4.4:78384312-78384331 MS.gene50341:intron 45.0%
! TTGATGACAAAGCCTGTGGA+GGG - chr4.4:78385075-78385094 MS.gene50341:intron 45.0%
!! AAGGATTCTTGGTGCTGCTT+TGG - chr4.4:78384528-78384547 MS.gene50341:CDS 45.0%
!! GCAAATGGTTCTAGTTCTCC+AGG + chr4.4:78386374-78386393 None:intergenic 45.0%
!!! GAGTTTTGGAAGCAACCAGA+TGG - chr4.4:78384355-78384374 MS.gene50341:intron 45.0%
AAATATGGGCCATGCGCTTC+AGG - chr4.4:78386472-78386491 MS.gene50341:CDS 50.0%
ACCTTGCTAGTCCTGAGTTC+CGG + chr4.4:78387101-78387120 None:intergenic 50.0%
AGTCGTTGCACATGCACTCT+GGG + chr4.4:78387151-78387170 None:intergenic 50.0%
CCGTTCGCGCTATCTCAAAA+GGG - chr4.4:78385131-78385150 MS.gene50341:CDS 50.0%
CTGGAGGAATGAATCAGCAG+AGG - chr4.4:78384476-78384495 MS.gene50341:CDS 50.0%
CTTGGAGTGACATGAAGAGG+AGG + chr4.4:78385105-78385124 None:intergenic 50.0%
GAATGAGAGGAGAGAACGAC+CGG - chr4.4:78385749-78385768 MS.gene50341:intron 50.0%
GAGGAGGAAGAAAAGGGTGA+GGG - chr4.4:78384319-78384338 MS.gene50341:intron 50.0%
GCAAGAATTTATTGGGCCGG+GGG - chr4.4:78386266-78386285 MS.gene50341:intron 50.0%
GCGAAGCCAGTTCAAGAACT+TGG - chr4.4:78385597-78385616 MS.gene50341:intron 50.0%
GGCTTATACCCTAACCCATC+TGG + chr4.4:78384373-78384392 None:intergenic 50.0%
GGTAACATCCATGGCAGTCA+AGG + chr4.4:78385835-78385854 None:intergenic 50.0%
GGTTCCTAGTGGAGTTGACT+TGG - chr4.4:78384297-78384316 MS.gene50341:intron 50.0%
GTTATTCCTTCCAGGGTCAC+AGG - chr4.4:78386943-78386962 MS.gene50341:CDS 50.0%
TCCGTTCGCGCTATCTCAAA+AGG - chr4.4:78385130-78385149 MS.gene50341:CDS 50.0%
TCCTCCAAGTCAACTCCACT+AGG + chr4.4:78384304-78384323 None:intergenic 50.0%
TCTTTGTCCCTTGACTGCCA+TGG - chr4.4:78385824-78385843 MS.gene50341:CDS 50.0%
TGACTGCCATGGATGTTACC+AGG - chr4.4:78385835-78385854 MS.gene50341:CDS 50.0%
TGGAAAAGGATGTGTGGGTG+AGG - chr4.4:78385679-78385698 MS.gene50341:intron 50.0%
! AACTCAGGACTAGCAAGGTC+GGG - chr4.4:78387102-78387121 MS.gene50341:CDS 50.0%
! CTTGATGACAAAGCCTGTGG+AGG - chr4.4:78385074-78385093 MS.gene50341:intron 50.0%
! GAACTCAGGACTAGCAAGGT+CGG - chr4.4:78387101-78387120 MS.gene50341:CDS 50.0%
! TGGAAGCAACCAGATGGGTT+AGG - chr4.4:78384361-78384380 MS.gene50341:intron 50.0%
!! GGAAGCAACCAGATGGGTTA+GGG - chr4.4:78384362-78384381 MS.gene50341:intron 50.0%
!! TAGTGGAGTTGACTTGGAGG+AGG - chr4.4:78384303-78384322 MS.gene50341:intron 50.0%
!! TCCTAGTGGAGTTGACTTGG+AGG - chr4.4:78384300-78384319 MS.gene50341:intron 50.0%
!!! CCCTTTTGAGATAGCGCGAA+CGG + chr4.4:78385134-78385153 None:intergenic 50.0%
AAGGATGTGTGGGTGAGGAC+TGG - chr4.4:78385684-78385703 MS.gene50341:intron 55.0%
CATTAGGACAGCCGGAACTC+AGG - chr4.4:78387087-78387106 MS.gene50341:CDS 55.0%
CGAAACCACCCGCACATCAT+TGG + chr4.4:78385037-78385056 None:intergenic 55.0%
GAACGACCGGAGCTCTTGAA+AGG - chr4.4:78385762-78385781 MS.gene50341:intron 55.0%
GAGTCGTTGCACATGCACTC+TGG + chr4.4:78387152-78387171 None:intergenic 55.0%
GGAGGAGGAAGAAAAGGGTG+AGG - chr4.4:78384318-78384337 MS.gene50341:intron 55.0%
TAGCGCGAACGGATCCAACT+TGG + chr4.4:78385123-78385142 None:intergenic 55.0%
TCTTGCCAATGATGTGCGGG+TGG - chr4.4:78385029-78385048 MS.gene50341:intron 55.0%
!! GTGAGGACTGGTTGTCTACC+CGG - chr4.4:78385696-78385715 MS.gene50341:intron 55.0%
!! TTGCACATGCACTCTGGGAC+AGG + chr4.4:78387146-78387165 None:intergenic 55.0%
AGAGGAGGACTTCCCTCCAC+AGG + chr4.4:78385090-78385109 None:intergenic 60.0%
ATAGGCCCTGTGACCCTGGA+AGG + chr4.4:78386952-78386971 None:intergenic 60.0%
TCCACCCAATGGTTGTCCCC+CGG + chr4.4:78386285-78386304 None:intergenic 60.0%
! CAGGACTAGCAAGGTCGGGA+GGG - chr4.4:78387106-78387125 MS.gene50341:CDS 60.0%
! GCCGGAACTCAGGACTAGCA+AGG - chr4.4:78387097-78387116 MS.gene50341:CDS 60.0%
! TCAGGACTAGCAAGGTCGGG+AGG - chr4.4:78387105-78387124 MS.gene50341:CDS 60.0%
CAGGGTCACAGGGCCTATGC+AGG - chr4.4:78386954-78386973 MS.gene50341:CDS 65.0%
CTGCATAGGCCCTGTGACCC+TGG + chr4.4:78386956-78386975 None:intergenic 65.0%
TGGGCCGGGGGACAACCATT+GGG - chr4.4:78386278-78386297 MS.gene50341:intron 65.0%
TTGGGCCGGGGGACAACCAT+TGG - chr4.4:78386277-78386296 MS.gene50341:intron 65.0%
GCCGGGGGACAACCATTGGG+TGG - chr4.4:78386281-78386300 MS.gene50341:intron 70.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 78383752 78387187 78383752 ID=MS.gene50341
chr4.4 mRNA 78383752 78387187 78383752 ID=MS.gene50341.t1;Parent=MS.gene50341
chr4.4 exon 78386346 78387187 78386346 ID=MS.gene50341.t1.exon1;Parent=MS.gene50341.t1
chr4.4 CDS 78386346 78387187 78386346 ID=cds.MS.gene50341.t1;Parent=MS.gene50341.t1
chr4.4 exon 78385787 78385952 78385787 ID=MS.gene50341.t1.exon2;Parent=MS.gene50341.t1
chr4.4 CDS 78385787 78385952 78385787 ID=cds.MS.gene50341.t1;Parent=MS.gene50341.t1
chr4.4 exon 78385451 78385540 78385451 ID=MS.gene50341.t1.exon3;Parent=MS.gene50341.t1
chr4.4 CDS 78385451 78385540 78385451 ID=cds.MS.gene50341.t1;Parent=MS.gene50341.t1
chr4.4 exon 78385083 78385366 78385083 ID=MS.gene50341.t1.exon4;Parent=MS.gene50341.t1
chr4.4 CDS 78385083 78385366 78385083 ID=cds.MS.gene50341.t1;Parent=MS.gene50341.t1
chr4.4 exon 78384446 78384704 78384446 ID=MS.gene50341.t1.exon5;Parent=MS.gene50341.t1
chr4.4 CDS 78384446 78384704 78384446 ID=cds.MS.gene50341.t1;Parent=MS.gene50341.t1
chr4.4 exon 78383752 78383982 78383752 ID=MS.gene50341.t1.exon6;Parent=MS.gene50341.t1
chr4.4 CDS 78383752 78383982 78383752 ID=cds.MS.gene50341.t1;Parent=MS.gene50341.t1
Gene Sequence

>MS.gene50341

ATGGCTAAGTCAAAGAACAATGCAAAGAAACTCTCATACATCTCAGTTCCTTCACAAATAATCAATTCCATTTCATCTTCTTCTATTCAATCATTACTTGATGATCAAACTCCAAAGAAATCTTCATCAAAAATTGCTTCTAACTTCTTCTTCACCACCTTAACAAAATGGAAAAACCCTTCAAGATTGTTCTTCTTTTTCACACTCTTTTTTCTTGGTGGTTTTGCTTTGTTTATGATTGGTTTCAATCTTAAAACACCGTTTTTTAAAACCCCATGTGCTACAAATGTTGAAAAGTCCTTAATCTCAAATGGGTTTGGTTCAAAATCTGAACTTAAGGTTGTTTCAATGAATAAAGATGAGATTTTTAATGGTGATGTTTTGGTAAAATCTGAACTTGGGGTTGTTCAAAACCCTTCAATCTCAAATGGGTTTGATTCAAAATCTGAACTTGGTGCTGTTTCAATGAATAAAGATGAGGTTTTGAATGATGTTAGTTTGTCAAATTCACTTCCTTTGATTGGTAATGTAATGTTGCAAGCAAAGGTTCCTAGTGGAGTTGACTTGGAGGAGGAAGAAAAGGGTGAGGGAGTTGAAAAGGGTGAGTTTTGGAAGCAACCAGATGGGTTAGGGTATAAGCCTTGTTTGGATTTCAGTAGGGATTATAGAAGAGAAAGTGACGGGATTGTGAAGAATAGGAAAAGGTATCTTATGGTAGTTGTTTCTGGAGGAATGAATCAGCAGAGGAATCAAATTGTTGATGCTGTTGTTATTGCAAGGATTCTTGGTGCTGCTTTGGTTGTTCCTATTTTGCAAGTTAATGTCATTTGGGGAGATGAAAGGTAAAGTTTACTATTCTTCTTGTTCATGGTTTGTGATATTGTAAGTGATGATTTTAATCAATGAAGATTTATGTAAGGTTTAGTTATTACAATTTTGGACTTAATCTAAGCCTAGGCATAGATTGATCTTGTTTCAATATTGTTTGAAAGACCAAATTTGGATATATATTTAGCTGTTACAAATTTGGACTGAATCTTGAGAAAGAACAACTTTGAAGCTTAAGCTTTAAGCATAGTTCTATTCTAGCTATAGTTTCAATCTTTTTGTTCTTGATACTGGTTATGTTTCGTTGTTAATGTTGATTAATTTCTTTCCTTATTTGTGAATGCTAGAAGAGAGTTTTCTGTAATTTATTATATTTTTTTCTCATTGGTTTTGGTTTTGTTTTGCAGTGAATTTGCTGATATATTTGATTTTGAGCACTTTAAGAATGTTCTTGCCAATGATGTGCGGGTGGTTTCGTCATTGCCATCAACACACTTGATGACAAAGCCTGTGGAGGGAAGTCCTCCTCTTCATGTCACTCCAAGTTGGATCCGTTCGCGCTATCTCAAAAGGGTAAGATTTTTTTGTTAATTACTCATGTTTTTGCATCTTTAGGTTTCAGTCCTAAGTTGTTGCTAAGTTTTTGTCTACTTTTGCGTTGAAAAATTTCAGACAATGATTGTCCCTTTGATGAGAAAGTGTGTTAACTTGTATAAATCATGTTAACTAATGAAAGCTTTGACTTCTGTGGAAATAAAAAATTGGGCAGTGAGTGATTGTTGAGCTTGAGTTTAACATGGTCTTTGTTGTCCTTTGCAGTTCAACAGAGAAGGTGTTTTGCTTTTACGCAGCTTGGATTCAAGGCTATCAAAGGATCTTCCTTCAGATCTTCAGAAGCTTCGATGCAAGGTATTGTGAAGTGTCTATAGTATCTTCATCTGTGTAACAATCGGTAGCATATTCTAAATGTTCTCTGCTCACCTATGTTTTCAGGTTGCTTTTAGTGCATTGAGATTTGCGAAGCCAGTTCAAGAACTTGGAAACAAGATTGCTGAGAGAATGCAAAGCAAAGGACCCTACCTTGCTCTTCATCTAAGAATGGAAAAGGATGTGTGGGTGAGGACTGGTTGTCTACCCGGTCTAAGTCCTGAGTTTGATGAGATTGTAAAGAATGAGAGGAGAGAACGACCGGAGCTCTTGAAAGGAAGATCAAACATGACTTACCATGAAAGAAAGATGGCTGGTCTTTGTCCCTTGACTGCCATGGATGTTACCAGGTAAATATTAAATTCTTCTACTTTTGTATAGGAGTCTGTTTGGATTCTCTTATTCGAGTTTATTACTGGCATTTACACTTATGAAACTATTTGGGAGAGCTTATGGAAACTTATGAAACGTCCATAAACTGTTTCCAGCTTATTCCCACAAGATCTCCAAGATAGCTTATGAAATCAGTTTAGTTTATATAAAAATAGTTAGATTTTATTTTATCTTTTATTATAGAAATAGCGTATACATAAACACTTATATGATAAACACTTATGCTACAAGCGCTCAATTAAGTTGTTTATCCAAAGATCCTATAGGCTTCACTTTTCAGTGGTTTTCTGAATATTAGTGTGACAGAGTTTTGTTTATTTGTTTTTGATTTATAGGTTGCTTAAAGCTTTAGGAGCTCAAAAAGATGCAAGAATTTATTGGGCCGGGGGACAACCATTGGGTGGAAAAGAAGCTTTGCATCCATTAATCCATGAGTTTCCACATCTTTATAATAAGGAAGATCTTGCATTGCCTGGAGAACTAGAACCATTTGCGAAGAAAGCTTCTCTAATGGCTGCCATAGATTACATAGTTTCTGAGAAGAGTGATGTTTTCATGCCATCTCATGGAGGAAATATGGGCCATGCGCTTCAGGTATGACTATATAATGACAGCAAGAGTGCTTTGCAAATAAGATACTCACATAAGCATTGATGATTGTTGCATAACTATATTGTAATCATGCTTACATATATTGGCCATGCATTCATATATACTGTATTCACACATATTTTATAAGAAAAGATTGCGACCTTTCAACTTTAGTCCCTATAAAATTTGTACTCACCTTTAGTCTTCATATTTAATAAAAATAGTGTGCTTTTAGTAATTAAAAAGGACTGATGATACAATCAATTATTTTAGGGATGTTGATGGGTCGTGATTTTATAGGACCAAAAACATATTTTCTCCATAAATTGTAATCATGCAATTAAGATAGTCACATTGATGGATGTTGCATAAATATATTGTAAATGTAATATTGCATACGCATATTTAATGTAGATTCTTCATTTGACAATTTCTAATATCTGAACCTAAATGTTATTCCTTCCAGGGTCACAGGGCCTATGCAGGACACAAGAAATACATAACCCCAAACAAAAGACAAATGCATCCCTATTTTCTGAATTCTTCTCTCCCTGAAGCAGAGTTTAACAGCATTGTCAAAGAATTGCATCAGAATTCATTAGGACAGCCGGAACTCAGGACTAGCAAGGTCGGGAGGGATGTCACGAAGTATCCTGTCCCAGAGTGCATGTGCAACGACTCACATAATGATTCGTAG
Protein sequence

>MS.gene50341.t1

MAKSKNNAKKLSYISVPSQIINSISSSSIQSLLDDQTPKKSSSKIASNFFFTTLTKWKNPSRLFFFFTLFFLGGFALFMIGFNLKTPFFKTPCATNVEKSLISNGFGSKSELKVVSMNKDEIFNGDVLVKSELGVVQNPSISNGFDSKSELGAVSMNKDEVLNDVSLSNSLPLIGNVMLQAKVPSGVDLEEEEKGEGVEKGEFWKQPDGLGYKPCLDFSRDYRRESDGIVKNRKRYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFADIFDFEHFKNVLANDVRVVSSLPSTHLMTKPVEGSPPLHVTPSWIRSRYLKRFNREGVLLLRSLDSRLSKDLPSDLQKLRCKVAFSALRFAKPVQELGNKIAERMQSKGPYLALHLRMEKDVWVRTGCLPGLSPEFDEIVKNERRERPELLKGRSNMTYHERKMAGLCPLTAMDVTRLLKALGAQKDARIYWAGGQPLGGKEALHPLIHEFPHLYNKEDLALPGELEPFAKKASLMAAIDYIVSEKSDVFMPSHGGNMGHALQGHRAYAGHKKYITPNKRQMHPYFLNSSLPEAEFNSIVKELHQNSLGQPELRTSKVGRDVTKYPVPECMCNDSHNDS