Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50922.t1 | XP_003598896.1 | 97 | 169 | 5 | 0 | 1 | 169 | 1 | 169 | 1.70E-89 | 338.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50922.t1 | Q39096 | 41.7 | 175 | 84 | 7 | 1 | 169 | 1 | 163 | 1.1e-23 | 111.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50922.t1 | B7FH52 | 97.0 | 169 | 5 | 0 | 1 | 169 | 1 | 169 | 1.2e-89 | 338.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053083 | MS.gene50922 | 0.809112 | 2.22E-50 | -1.69E-46 |
| MS.gene057989 | MS.gene50922 | 0.918057 | 2.49E-86 | -1.69E-46 |
| MS.gene057992 | MS.gene50922 | 0.944417 | 2.03E-103 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50922.t1 | MTR_3g023110 | 97.041 | 169 | 5 | 0 | 1 | 169 | 1 | 169 | 1.99e-122 | 342 |
| MS.gene50922.t1 | MTR_7g090630 | 49.524 | 105 | 49 | 3 | 1 | 104 | 1 | 102 | 1.87e-28 | 102 |
| MS.gene50922.t1 | MTR_7g090630 | 50.000 | 104 | 48 | 3 | 1 | 103 | 1 | 101 | 5.39e-28 | 100 |
| MS.gene50922.t1 | MTR_5g083690 | 32.749 | 171 | 73 | 4 | 1 | 169 | 4 | 134 | 1.81e-20 | 82.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50922.t1 | AT2G41430 | 41.143 | 175 | 85 | 6 | 1 | 169 | 1 | 163 | 1.79e-28 | 103 |
| MS.gene50922.t1 | AT2G41430 | 41.143 | 175 | 85 | 6 | 1 | 169 | 1 | 163 | 1.79e-28 | 103 |
| MS.gene50922.t1 | AT2G41430 | 41.143 | 175 | 85 | 6 | 1 | 169 | 1 | 163 | 1.79e-28 | 103 |
| MS.gene50922.t1 | AT2G41430 | 41.143 | 175 | 85 | 6 | 1 | 169 | 1 | 163 | 1.79e-28 | 103 |
| MS.gene50922.t1 | AT2G41430 | 53.750 | 80 | 34 | 2 | 1 | 77 | 1 | 80 | 3.84e-24 | 91.7 |
| MS.gene50922.t1 | AT4G14270 | 34.659 | 176 | 72 | 7 | 2 | 169 | 3 | 143 | 9.11e-24 | 91.3 |
| MS.gene50922.t1 | AT4G14270 | 34.659 | 176 | 72 | 7 | 2 | 169 | 3 | 143 | 9.11e-24 | 91.3 |
Find 37 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATAATGAGTTTGAGAAAT+TGG | 0.215472 | 3.3:+13866885 | MS.gene50922:CDS |
| CGACATGGTTTCATAGCTAT+TGG | 0.303499 | 3.3:+13866737 | MS.gene50922:CDS |
| GACTTTGGACCCCTTTCCTT+TGG | 0.317537 | 3.3:-13867724 | None:intergenic |
| GCTGGCTTCTCAAAGTGCTT+TGG | 0.365521 | 3.3:-13867760 | None:intergenic |
| GTTCTGATGGCATTGGTAAC+TGG | 0.375492 | 3.3:+13866613 | None:intergenic |
| AAGTGCTTTGGAGATCCAAT+AGG | 0.400879 | 3.3:-13867748 | None:intergenic |
| ATTCAATGCTTACCATGAAC+AGG | 0.405358 | 3.3:-13866967 | None:intergenic |
| CGAGGTTGGTGAATTCTTTG+AGG | 0.416409 | 3.3:-13867808 | None:intergenic |
| GGAGATCCAATAGGGGACTT+TGG | 0.419975 | 3.3:-13867739 | None:intergenic |
| GGTATGAAGAGAGGTGCATT+TGG | 0.424438 | 3.3:-13866655 | None:intergenic |
| TCAACAGGATCATCCATAAC+TGG | 0.427432 | 3.3:-13866934 | None:intergenic |
| AGGGGTCCAAAGTCCCCTAT+TGG | 0.433062 | 3.3:+13867733 | MS.gene50922:CDS |
| AGTGCTTTGGAGATCCAATA+GGG | 0.437450 | 3.3:-13867747 | None:intergenic |
| AAGAATTCACCAACCTCGTT+AGG | 0.440467 | 3.3:+13867813 | MS.gene50922:CDS |
| AGCTCAAGAGTGTCCAGTTA+TGG | 0.459111 | 3.3:+13866921 | MS.gene50922:CDS |
| GATCTTGTCACCAAAGGAAA+GGG | 0.460246 | 3.3:+13867714 | MS.gene50922:CDS |
| TGGACACTCTTGAGCTTCAT+CGG | 0.502009 | 3.3:-13866914 | None:intergenic |
| CTATTGGTTGAGCCAGCATA+AGG | 0.514718 | 3.3:+13866753 | MS.gene50922:CDS |
| TTCTTTGAGGTGCACACTTG+AGG | 0.519384 | 3.3:-13867795 | None:intergenic |
| CAAAATCTTCCTCCTTATGC+TGG | 0.523223 | 3.3:-13866765 | None:intergenic |
| TTGAGGTTAACATATTGTGC+TGG | 0.533520 | 3.3:-13867778 | None:intergenic |
| ACCTGCTGCATTTCGTCAAG+TGG | 0.534431 | 3.3:+13866675 | MS.gene50922:CDS |
| TTCACAAGCTCCCACCATTG+AGG | 0.540449 | 3.3:-13866709 | None:intergenic |
| AAAGTTTATCCTAACGAGGT+TGG | 0.543754 | 3.3:-13867822 | None:intergenic |
| TCCACTTGACGAAATGCAGC+AGG | 0.554768 | 3.3:-13866676 | None:intergenic |
| TTCTTAATCAGGTGTTAACA+AGG | 0.557343 | 3.3:+13867672 | MS.gene50922:intron |
| AGATCTTGTCACCAAAGGAA+AGG | 0.561565 | 3.3:+13867713 | MS.gene50922:CDS |
| CATCAAGATCTTGTCACCAA+AGG | 0.566700 | 3.3:+13867708 | MS.gene50922:CDS |
| TGCTGCATTTCGTCAAGTGG+AGG | 0.583030 | 3.3:+13866678 | MS.gene50922:CDS |
| CTTCAAAGTTTATCCTAACG+AGG | 0.589028 | 3.3:-13867826 | None:intergenic |
| AGCTTGTGAAATCTTCGACA+TGG | 0.599712 | 3.3:+13866722 | MS.gene50922:CDS |
| CTGACAAGTTCTGATGGCAT+TGG | 0.609871 | 3.3:+13866606 | None:intergenic |
| AGTTATGGATGATCCTGTTG+AGG | 0.618736 | 3.3:+13866936 | MS.gene50922:CDS |
| TTGGTTGAGCCAGCATAAGG+AGG | 0.633628 | 3.3:+13866756 | MS.gene50922:CDS |
| ATCTTGTCACCAAAGGAAAG+GGG | 0.652126 | 3.3:+13867715 | MS.gene50922:CDS |
| AATGCAGCAGGTATGAAGAG+AGG | 0.675950 | 3.3:-13866664 | None:intergenic |
| GTGCTTTGGAGATCCAATAG+GGG | 0.692880 | 3.3:-13867746 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATAATGATCAAATATAAA+CGG | - | chr3.3:13867021-13867040 | None:intergenic | 10.0% |
| !! | TAGTTTAAAAATCATACAAA+GGG | + | chr3.3:13867340-13867359 | MS.gene50922:intron | 15.0% |
| !!! | AATACTTTTTCAAACTAAAT+AGG | + | chr3.3:13867239-13867258 | MS.gene50922:intron | 15.0% |
| !!! | ATTATATTTACTCTAACTTT+TGG | - | chr3.3:13867527-13867546 | None:intergenic | 15.0% |
| !! | CTAGTTTAAAAATCATACAA+AGG | + | chr3.3:13867339-13867358 | MS.gene50922:intron | 20.0% |
| !!! | AATTGATTTCTTTCTTAATC+AGG | + | chr3.3:13867661-13867680 | MS.gene50922:intron | 20.0% |
| !!! | AGATCAATTTTATACATTGT+CGG | - | chr3.3:13867409-13867428 | None:intergenic | 20.0% |
| ! | AGATAATGAGTTTGAGAAAT+TGG | + | chr3.3:13866885-13866904 | MS.gene50922:CDS | 25.0% |
| !! | GAATCGAACTCAAAATTTTA+GGG | - | chr3.3:13867611-13867630 | None:intergenic | 25.0% |
| !! | TTACACATTTTGTGATTAGT+AGG | - | chr3.3:13867633-13867652 | None:intergenic | 25.0% |
| !! | TTTTAAACACAGTAGATTCT+AGG | - | chr3.3:13867194-13867213 | None:intergenic | 25.0% |
| GTGTACAAATCTTTGAACAT+AGG | + | chr3.3:13867067-13867086 | MS.gene50922:intron | 30.0% | |
| TATAATGTGTCATACCACAA+AGG | + | chr3.3:13867541-13867560 | MS.gene50922:intron | 30.0% | |
| TGATACAACGTTATATGTCT+TGG | + | chr3.3:13867292-13867311 | MS.gene50922:intron | 30.0% | |
| TTCTTAATCAGGTGTTAACA+AGG | + | chr3.3:13867672-13867691 | MS.gene50922:intron | 30.0% | |
| TTGGATTTAATCGATATGCA+CGG | + | chr3.3:13867311-13867330 | MS.gene50922:intron | 30.0% | |
| ! | GGAATCGAACTCAAAATTTT+AGG | - | chr3.3:13867612-13867631 | None:intergenic | 30.0% |
| ATTCAATGCTTACCATGAAC+AGG | - | chr3.3:13866970-13866989 | None:intergenic | 35.0% | |
| CCTTGAGATATCCAAAAAAG+TGG | + | chr3.3:13867263-13867282 | MS.gene50922:intron | 35.0% | |
| GATATCCAAAAAAGTGGATG+TGG | + | chr3.3:13867269-13867288 | MS.gene50922:intron | 35.0% | |
| TTGAGGTTAACATATTGTGC+TGG | - | chr3.3:13867781-13867800 | None:intergenic | 35.0% | |
| ! | CTGTCAGAAACTTTTGATCT+TGG | + | chr3.3:13866838-13866857 | MS.gene50922:CDS | 35.0% |
| !!! | CCACTTTTTTGGATATCTCA+AGG | - | chr3.3:13867266-13867285 | None:intergenic | 35.0% |
| !!! | TATCACCACATCCACTTTTT+TGG | - | chr3.3:13867277-13867296 | None:intergenic | 35.0% |
| AGATCTTGTCACCAAAGGAA+AGG | + | chr3.3:13867713-13867732 | MS.gene50922:CDS | 40.0% | |
| AGCTTGTGAAATCTTCGACA+TGG | + | chr3.3:13866722-13866741 | MS.gene50922:CDS | 40.0% | |
| AGTTATGGATGATCCTGTTG+AGG | + | chr3.3:13866936-13866955 | MS.gene50922:CDS | 40.0% | |
| ATCTTGTCACCAAAGGAAAG+GGG | + | chr3.3:13867715-13867734 | MS.gene50922:CDS | 40.0% | |
| CAAAATCTTCCTCCTTATGC+TGG | - | chr3.3:13866768-13866787 | None:intergenic | 40.0% | |
| CATCAAGATCTTGTCACCAA+AGG | + | chr3.3:13867708-13867727 | MS.gene50922:CDS | 40.0% | |
| CGACATGGTTTCATAGCTAT+TGG | + | chr3.3:13866737-13866756 | MS.gene50922:CDS | 40.0% | |
| GAGGAAAAAAAGCCTGTTCA+TGG | + | chr3.3:13866955-13866974 | MS.gene50922:CDS | 40.0% | |
| GATCTGTGTTTGTGTTTGGT+CGG | + | chr3.3:13867364-13867383 | MS.gene50922:intron | 40.0% | |
| GATCTTGTCACCAAAGGAAA+GGG | + | chr3.3:13867714-13867733 | MS.gene50922:CDS | 40.0% | |
| TCAACAGGATCATCCATAAC+TGG | - | chr3.3:13866937-13866956 | None:intergenic | 40.0% | |
| ! | AAAGGTGTAGTGCAAGTAGT+TGG | + | chr3.3:13867559-13867578 | MS.gene50922:intron | 40.0% |
| ! | AGGATTTTTCACCTCAATGG+TGG | + | chr3.3:13866698-13866717 | MS.gene50922:CDS | 40.0% |
| ! | GAAGATTTTGTCGAAAGTGG+CGG | + | chr3.3:13866778-13866797 | MS.gene50922:CDS | 40.0% |
| ! | GAGGAAGATTTTGTCGAAAG+TGG | + | chr3.3:13866775-13866794 | MS.gene50922:CDS | 40.0% |
| ! | GGATTTTTCACCTCAATGGT+GGG | + | chr3.3:13866699-13866718 | MS.gene50922:CDS | 40.0% |
| ! | TGGAGGATTTTTCACCTCAA+TGG | + | chr3.3:13866695-13866714 | MS.gene50922:CDS | 40.0% |
| !! | AAGTGCTTTGGAGATCCAAT+AGG | - | chr3.3:13867751-13867770 | None:intergenic | 40.0% |
| !! | AGTGCTTTGGAGATCCAATA+GGG | - | chr3.3:13867750-13867769 | None:intergenic | 40.0% |
| !!! | ACAGGCTTTTTTTCCTCAAC+AGG | - | chr3.3:13866952-13866971 | None:intergenic | 40.0% |
| AATGCAGCAGGTATGAAGAG+AGG | - | chr3.3:13866667-13866686 | None:intergenic | 45.0% | |
| CTACTTGCACTACACCTTTG+TGG | - | chr3.3:13867558-13867577 | None:intergenic | 45.0% | |
| GGGTGATCTGTGTTTGTGTT+TGG | + | chr3.3:13867360-13867379 | MS.gene50922:intron | 45.0% | |
| TGGACACTCTTGAGCTTCAT+CGG | - | chr3.3:13866917-13866936 | None:intergenic | 45.0% | |
| ! | CTATTGGTTGAGCCAGCATA+AGG | + | chr3.3:13866753-13866772 | MS.gene50922:CDS | 45.0% |
| ! | GGTATGAAGAGAGGTGCATT+TGG | - | chr3.3:13866658-13866677 | None:intergenic | 45.0% |
| !! | AGCTCAAGAGTGTCCAGTTA+TGG | + | chr3.3:13866921-13866940 | MS.gene50922:CDS | 45.0% |
| !! | CGAGGTTGGTGAATTCTTTG+AGG | - | chr3.3:13867811-13867830 | None:intergenic | 45.0% |
| !! | GTGCTTTGGAGATCCAATAG+GGG | - | chr3.3:13867749-13867768 | None:intergenic | 45.0% |
| !! | TTCTTTGAGGTGCACACTTG+AGG | - | chr3.3:13867798-13867817 | None:intergenic | 45.0% |
| ACCTGCTGCATTTCGTCAAG+TGG | + | chr3.3:13866675-13866694 | MS.gene50922:CDS | 50.0% | |
| GACTTTGGACCCCTTTCCTT+TGG | - | chr3.3:13867727-13867746 | None:intergenic | 50.0% | |
| GGAGATCCAATAGGGGACTT+TGG | - | chr3.3:13867742-13867761 | None:intergenic | 50.0% | |
| TCCACTTGACGAAATGCAGC+AGG | - | chr3.3:13866679-13866698 | None:intergenic | 50.0% | |
| TGCTGCATTTCGTCAAGTGG+AGG | + | chr3.3:13866678-13866697 | MS.gene50922:CDS | 50.0% | |
| TTCACAAGCTCCCACCATTG+AGG | - | chr3.3:13866712-13866731 | None:intergenic | 50.0% | |
| TTGGTTGAGCCAGCATAAGG+AGG | + | chr3.3:13866756-13866775 | MS.gene50922:CDS | 50.0% | |
| ! | GCTGGCTTCTCAAAGTGCTT+TGG | - | chr3.3:13867763-13867782 | None:intergenic | 50.0% |
| AGGGGTCCAAAGTCCCCTAT+TGG | + | chr3.3:13867733-13867752 | MS.gene50922:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 13866619 | 13867834 | 13866619 | ID=MS.gene50922 |
| chr3.3 | mRNA | 13866619 | 13867834 | 13866619 | ID=MS.gene50922.t1;Parent=MS.gene50922 |
| chr3.3 | exon | 13866619 | 13866976 | 13866619 | ID=MS.gene50922.t1.exon1;Parent=MS.gene50922.t1 |
| chr3.3 | CDS | 13866619 | 13866976 | 13866619 | ID=cds.MS.gene50922.t1;Parent=MS.gene50922.t1 |
| chr3.3 | exon | 13867683 | 13867834 | 13867683 | ID=MS.gene50922.t1.exon2;Parent=MS.gene50922.t1 |
| chr3.3 | CDS | 13867683 | 13867834 | 13867683 | ID=cds.MS.gene50922.t1;Parent=MS.gene50922.t1 |
| Gene Sequence |
| Protein sequence |
