Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50926.t1 | XP_003598896.1 | 97 | 169 | 5 | 0 | 1 | 169 | 1 | 169 | 1.70E-89 | 338.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50926.t1 | Q39096 | 41.7 | 175 | 84 | 7 | 1 | 169 | 1 | 163 | 1.1e-23 | 111.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50926.t1 | B7FH52 | 97.0 | 169 | 5 | 0 | 1 | 169 | 1 | 169 | 1.2e-89 | 338.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053083 | MS.gene50926 | 0.80953 | 1.81E-50 | -1.69E-46 |
| MS.gene057989 | MS.gene50926 | 0.921012 | 6.17E-88 | -1.69E-46 |
| MS.gene057992 | MS.gene50926 | 0.944502 | 1.73E-103 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50926.t1 | MTR_3g023110 | 97.041 | 169 | 5 | 0 | 1 | 169 | 1 | 169 | 1.99e-122 | 342 |
| MS.gene50926.t1 | MTR_7g090630 | 49.524 | 105 | 49 | 3 | 1 | 104 | 1 | 102 | 1.87e-28 | 102 |
| MS.gene50926.t1 | MTR_7g090630 | 50.000 | 104 | 48 | 3 | 1 | 103 | 1 | 101 | 5.39e-28 | 100 |
| MS.gene50926.t1 | MTR_5g083690 | 32.749 | 171 | 73 | 4 | 1 | 169 | 4 | 134 | 1.81e-20 | 82.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50926.t1 | AT2G41430 | 41.143 | 175 | 85 | 6 | 1 | 169 | 1 | 163 | 1.79e-28 | 103 |
| MS.gene50926.t1 | AT2G41430 | 41.143 | 175 | 85 | 6 | 1 | 169 | 1 | 163 | 1.79e-28 | 103 |
| MS.gene50926.t1 | AT2G41430 | 41.143 | 175 | 85 | 6 | 1 | 169 | 1 | 163 | 1.79e-28 | 103 |
| MS.gene50926.t1 | AT2G41430 | 41.143 | 175 | 85 | 6 | 1 | 169 | 1 | 163 | 1.79e-28 | 103 |
| MS.gene50926.t1 | AT2G41430 | 53.750 | 80 | 34 | 2 | 1 | 77 | 1 | 80 | 3.84e-24 | 91.7 |
| MS.gene50926.t1 | AT4G14270 | 34.659 | 176 | 72 | 7 | 2 | 169 | 3 | 143 | 9.11e-24 | 91.3 |
| MS.gene50926.t1 | AT4G14270 | 34.659 | 176 | 72 | 7 | 2 | 169 | 3 | 143 | 9.11e-24 | 91.3 |
Find 37 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATAATGAGTTTGAGAAAT+TGG | 0.215472 | 3.3:+13855613 | MS.gene50926:CDS |
| CGACATGGTTTCATAGCTAT+TGG | 0.303499 | 3.3:+13855465 | MS.gene50926:CDS |
| GACTTTGGACCCCTTTCCTT+TGG | 0.317537 | 3.3:-13856452 | None:intergenic |
| GCTGGCTTCTCAAAGTGCTT+TGG | 0.365521 | 3.3:-13856488 | None:intergenic |
| GTTCTGATGGCATTGGTAAC+TGG | 0.375492 | 3.3:+13855341 | None:intergenic |
| AAGTGCTTTGGAGATCCAAT+AGG | 0.400879 | 3.3:-13856476 | None:intergenic |
| ATTCAATGCTTACCATGAAC+AGG | 0.405358 | 3.3:-13855695 | None:intergenic |
| CGAGGTTGGTGAATTCTTTG+AGG | 0.416409 | 3.3:-13856536 | None:intergenic |
| GGAGATCCAATAGGGGACTT+TGG | 0.419975 | 3.3:-13856467 | None:intergenic |
| GGTATGAAGAGAGGTGCATT+TGG | 0.424438 | 3.3:-13855383 | None:intergenic |
| TCAACAGGATCATCCATAAC+TGG | 0.427432 | 3.3:-13855662 | None:intergenic |
| AGGGGTCCAAAGTCCCCTAT+TGG | 0.433062 | 3.3:+13856461 | MS.gene50926:CDS |
| AGTGCTTTGGAGATCCAATA+GGG | 0.437450 | 3.3:-13856475 | None:intergenic |
| AAGAATTCACCAACCTCGTT+AGG | 0.440467 | 3.3:+13856541 | MS.gene50926:CDS |
| AGCTCAAGAGTGTCCAGTTA+TGG | 0.459111 | 3.3:+13855649 | MS.gene50926:CDS |
| GATCTTGTCACCAAAGGAAA+GGG | 0.460246 | 3.3:+13856442 | MS.gene50926:CDS |
| TGGACACTCTTGAGCTTCAT+CGG | 0.502009 | 3.3:-13855642 | None:intergenic |
| CTATTGGTTGAGCCAGCATA+AGG | 0.514718 | 3.3:+13855481 | MS.gene50926:CDS |
| TTCTTTGAGGTGCACACTTG+AGG | 0.519384 | 3.3:-13856523 | None:intergenic |
| CAAAATCTTCCTCCTTATGC+TGG | 0.523223 | 3.3:-13855493 | None:intergenic |
| TTGAGGTTAACATATTGTGC+TGG | 0.533520 | 3.3:-13856506 | None:intergenic |
| ACCTGCTGCATTTCGTCAAG+TGG | 0.534431 | 3.3:+13855403 | MS.gene50926:CDS |
| TTCACAAGCTCCCACCATTG+AGG | 0.540449 | 3.3:-13855437 | None:intergenic |
| AAAGTTTATCCTAACGAGGT+TGG | 0.543754 | 3.3:-13856550 | None:intergenic |
| TCCACTTGACGAAATGCAGC+AGG | 0.554768 | 3.3:-13855404 | None:intergenic |
| TTCTTAATCAGGTGTTAACA+AGG | 0.557343 | 3.3:+13856400 | MS.gene50926:intron |
| AGATCTTGTCACCAAAGGAA+AGG | 0.561565 | 3.3:+13856441 | MS.gene50926:CDS |
| CATCAAGATCTTGTCACCAA+AGG | 0.566700 | 3.3:+13856436 | MS.gene50926:CDS |
| TGCTGCATTTCGTCAAGTGG+AGG | 0.583030 | 3.3:+13855406 | MS.gene50926:CDS |
| CTTCAAAGTTTATCCTAACG+AGG | 0.589028 | 3.3:-13856554 | None:intergenic |
| AGCTTGTGAAATCTTCGACA+TGG | 0.599712 | 3.3:+13855450 | MS.gene50926:CDS |
| CTGACAAGTTCTGATGGCAT+TGG | 0.609871 | 3.3:+13855334 | None:intergenic |
| AGTTATGGATGATCCTGTTG+AGG | 0.618736 | 3.3:+13855664 | MS.gene50926:CDS |
| TTGGTTGAGCCAGCATAAGG+AGG | 0.633628 | 3.3:+13855484 | MS.gene50926:CDS |
| ATCTTGTCACCAAAGGAAAG+GGG | 0.652126 | 3.3:+13856443 | MS.gene50926:CDS |
| AATGCAGCAGGTATGAAGAG+AGG | 0.675950 | 3.3:-13855392 | None:intergenic |
| GTGCTTTGGAGATCCAATAG+GGG | 0.692880 | 3.3:-13856474 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATAATGATCAAATATAAA+CGG | - | chr3.3:13855749-13855768 | None:intergenic | 10.0% |
| !! | TAGTTTAAAAATCATACAAA+GGG | + | chr3.3:13856068-13856087 | MS.gene50926:intron | 15.0% |
| !!! | AATACTTTTTCAAACTAAAT+AGG | + | chr3.3:13855967-13855986 | MS.gene50926:intron | 15.0% |
| !!! | ATTATATTTACTCTAACTTT+TGG | - | chr3.3:13856255-13856274 | None:intergenic | 15.0% |
| !! | CTAGTTTAAAAATCATACAA+AGG | + | chr3.3:13856067-13856086 | MS.gene50926:intron | 20.0% |
| !!! | AATTGATTTCTTTCTTAATC+AGG | + | chr3.3:13856389-13856408 | MS.gene50926:intron | 20.0% |
| !!! | AGATCAATTTTATACATTGT+CGG | - | chr3.3:13856137-13856156 | None:intergenic | 20.0% |
| ! | AGATAATGAGTTTGAGAAAT+TGG | + | chr3.3:13855613-13855632 | MS.gene50926:CDS | 25.0% |
| !! | GAATCGAACTCAAAATTTTA+GGG | - | chr3.3:13856339-13856358 | None:intergenic | 25.0% |
| !! | TTACACATTTTGTGATTAGT+AGG | - | chr3.3:13856361-13856380 | None:intergenic | 25.0% |
| !! | TTTTAAACACAGTAGATTCT+AGG | - | chr3.3:13855922-13855941 | None:intergenic | 25.0% |
| GTGTACAAATCTTTGAACAT+AGG | + | chr3.3:13855795-13855814 | MS.gene50926:intron | 30.0% | |
| TATAATGTGTCATACCACAA+AGG | + | chr3.3:13856269-13856288 | MS.gene50926:intron | 30.0% | |
| TGATACAACGTTATATGTCT+TGG | + | chr3.3:13856020-13856039 | MS.gene50926:intron | 30.0% | |
| TTCTTAATCAGGTGTTAACA+AGG | + | chr3.3:13856400-13856419 | MS.gene50926:intron | 30.0% | |
| TTGGATTTAATCGATATGCA+CGG | + | chr3.3:13856039-13856058 | MS.gene50926:intron | 30.0% | |
| ! | GGAATCGAACTCAAAATTTT+AGG | - | chr3.3:13856340-13856359 | None:intergenic | 30.0% |
| ATTCAATGCTTACCATGAAC+AGG | - | chr3.3:13855698-13855717 | None:intergenic | 35.0% | |
| CCTTGAGATATCCAAAAAAG+TGG | + | chr3.3:13855991-13856010 | MS.gene50926:intron | 35.0% | |
| GATATCCAAAAAAGTGGATG+TGG | + | chr3.3:13855997-13856016 | MS.gene50926:intron | 35.0% | |
| TTGAGGTTAACATATTGTGC+TGG | - | chr3.3:13856509-13856528 | None:intergenic | 35.0% | |
| ! | CTGTCAGAAACTTTTGATCT+TGG | + | chr3.3:13855566-13855585 | MS.gene50926:CDS | 35.0% |
| !!! | CCACTTTTTTGGATATCTCA+AGG | - | chr3.3:13855994-13856013 | None:intergenic | 35.0% |
| !!! | TATCACCACATCCACTTTTT+TGG | - | chr3.3:13856005-13856024 | None:intergenic | 35.0% |
| AGATCTTGTCACCAAAGGAA+AGG | + | chr3.3:13856441-13856460 | MS.gene50926:CDS | 40.0% | |
| AGCTTGTGAAATCTTCGACA+TGG | + | chr3.3:13855450-13855469 | MS.gene50926:CDS | 40.0% | |
| AGTTATGGATGATCCTGTTG+AGG | + | chr3.3:13855664-13855683 | MS.gene50926:CDS | 40.0% | |
| ATCTTGTCACCAAAGGAAAG+GGG | + | chr3.3:13856443-13856462 | MS.gene50926:CDS | 40.0% | |
| CAAAATCTTCCTCCTTATGC+TGG | - | chr3.3:13855496-13855515 | None:intergenic | 40.0% | |
| CATCAAGATCTTGTCACCAA+AGG | + | chr3.3:13856436-13856455 | MS.gene50926:CDS | 40.0% | |
| CGACATGGTTTCATAGCTAT+TGG | + | chr3.3:13855465-13855484 | MS.gene50926:CDS | 40.0% | |
| GAGGAAAAAAAGCCTGTTCA+TGG | + | chr3.3:13855683-13855702 | MS.gene50926:CDS | 40.0% | |
| GATCTGTGTTTGTGTTTGGT+CGG | + | chr3.3:13856092-13856111 | MS.gene50926:intron | 40.0% | |
| GATCTTGTCACCAAAGGAAA+GGG | + | chr3.3:13856442-13856461 | MS.gene50926:CDS | 40.0% | |
| TCAACAGGATCATCCATAAC+TGG | - | chr3.3:13855665-13855684 | None:intergenic | 40.0% | |
| ! | AAAGGTGTAGTGCAAGTAGT+TGG | + | chr3.3:13856287-13856306 | MS.gene50926:intron | 40.0% |
| ! | AGGATTTTTCACCTCAATGG+TGG | + | chr3.3:13855426-13855445 | MS.gene50926:CDS | 40.0% |
| ! | GAAGATTTTGTCGAAAGTGG+CGG | + | chr3.3:13855506-13855525 | MS.gene50926:CDS | 40.0% |
| ! | GAGGAAGATTTTGTCGAAAG+TGG | + | chr3.3:13855503-13855522 | MS.gene50926:CDS | 40.0% |
| ! | GGATTTTTCACCTCAATGGT+GGG | + | chr3.3:13855427-13855446 | MS.gene50926:CDS | 40.0% |
| ! | TGGAGGATTTTTCACCTCAA+TGG | + | chr3.3:13855423-13855442 | MS.gene50926:CDS | 40.0% |
| !! | AAGTGCTTTGGAGATCCAAT+AGG | - | chr3.3:13856479-13856498 | None:intergenic | 40.0% |
| !! | AGTGCTTTGGAGATCCAATA+GGG | - | chr3.3:13856478-13856497 | None:intergenic | 40.0% |
| !!! | ACAGGCTTTTTTTCCTCAAC+AGG | - | chr3.3:13855680-13855699 | None:intergenic | 40.0% |
| AATGCAGCAGGTATGAAGAG+AGG | - | chr3.3:13855395-13855414 | None:intergenic | 45.0% | |
| CTACTTGCACTACACCTTTG+TGG | - | chr3.3:13856286-13856305 | None:intergenic | 45.0% | |
| GGGTGATCTGTGTTTGTGTT+TGG | + | chr3.3:13856088-13856107 | MS.gene50926:intron | 45.0% | |
| TGGACACTCTTGAGCTTCAT+CGG | - | chr3.3:13855645-13855664 | None:intergenic | 45.0% | |
| ! | CTATTGGTTGAGCCAGCATA+AGG | + | chr3.3:13855481-13855500 | MS.gene50926:CDS | 45.0% |
| ! | GGTATGAAGAGAGGTGCATT+TGG | - | chr3.3:13855386-13855405 | None:intergenic | 45.0% |
| !! | AGCTCAAGAGTGTCCAGTTA+TGG | + | chr3.3:13855649-13855668 | MS.gene50926:CDS | 45.0% |
| !! | CGAGGTTGGTGAATTCTTTG+AGG | - | chr3.3:13856539-13856558 | None:intergenic | 45.0% |
| !! | GTGCTTTGGAGATCCAATAG+GGG | - | chr3.3:13856477-13856496 | None:intergenic | 45.0% |
| !! | TTCTTTGAGGTGCACACTTG+AGG | - | chr3.3:13856526-13856545 | None:intergenic | 45.0% |
| ACCTGCTGCATTTCGTCAAG+TGG | + | chr3.3:13855403-13855422 | MS.gene50926:CDS | 50.0% | |
| GACTTTGGACCCCTTTCCTT+TGG | - | chr3.3:13856455-13856474 | None:intergenic | 50.0% | |
| GGAGATCCAATAGGGGACTT+TGG | - | chr3.3:13856470-13856489 | None:intergenic | 50.0% | |
| TCCACTTGACGAAATGCAGC+AGG | - | chr3.3:13855407-13855426 | None:intergenic | 50.0% | |
| TGCTGCATTTCGTCAAGTGG+AGG | + | chr3.3:13855406-13855425 | MS.gene50926:CDS | 50.0% | |
| TTCACAAGCTCCCACCATTG+AGG | - | chr3.3:13855440-13855459 | None:intergenic | 50.0% | |
| TTGGTTGAGCCAGCATAAGG+AGG | + | chr3.3:13855484-13855503 | MS.gene50926:CDS | 50.0% | |
| ! | GCTGGCTTCTCAAAGTGCTT+TGG | - | chr3.3:13856491-13856510 | None:intergenic | 50.0% |
| AGGGGTCCAAAGTCCCCTAT+TGG | + | chr3.3:13856461-13856480 | MS.gene50926:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 13855347 | 13856562 | 13855347 | ID=MS.gene50926 |
| chr3.3 | mRNA | 13855347 | 13856562 | 13855347 | ID=MS.gene50926.t1;Parent=MS.gene50926 |
| chr3.3 | exon | 13855347 | 13855704 | 13855347 | ID=MS.gene50926.t1.exon1;Parent=MS.gene50926.t1 |
| chr3.3 | CDS | 13855347 | 13855704 | 13855347 | ID=cds.MS.gene50926.t1;Parent=MS.gene50926.t1 |
| chr3.3 | exon | 13856411 | 13856562 | 13856411 | ID=MS.gene50926.t1.exon2;Parent=MS.gene50926.t1 |
| chr3.3 | CDS | 13856411 | 13856562 | 13856411 | ID=cds.MS.gene50926.t1;Parent=MS.gene50926.t1 |
| Gene Sequence |
| Protein sequence |
