AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene51086

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51086.t1 MTR_5g083360 98.589 567 8 0 1 567 1 567 0.0 1164
MS.gene51086.t1 MTR_3g071890 81.489 524 86 3 4 523 10 526 0.0 886
MS.gene51086.t1 MTR_5g073210 59.155 568 223 4 1 567 1 560 0.0 726
MS.gene51086.t1 MTR_5g020620 50.265 565 261 5 7 567 13 561 0.0 603
MS.gene51086.t1 MTR_5g020600 50.890 562 254 7 12 567 21 566 0.0 593
MS.gene51086.t1 MTR_7g062250 51.839 571 256 9 7 567 17 578 0.0 590
MS.gene51086.t1 MTR_2g008330 48.691 573 281 6 1 567 14 579 0.0 585
MS.gene51086.t1 MTR_7g058690 51.693 561 254 10 17 567 27 580 0.0 582
MS.gene51086.t1 MTR_7g062310 51.449 552 253 8 25 567 48 593 0.0 582
MS.gene51086.t1 MTR_5g069680 49.204 565 267 7 7 567 10 558 0.0 580
MS.gene51086.t1 MTR_4g064530 52.102 547 246 9 12 551 32 569 0.0 578
MS.gene51086.t1 MTR_4g015120 49.387 571 271 6 1 567 1 557 0.0 575
MS.gene51086.t1 MTR_7g458880 49.739 575 269 10 6 567 13 580 0.0 574
MS.gene51086.t1 MTR_7g060460 50.000 566 259 11 16 567 26 581 0.0 568
MS.gene51086.t1 MTR_3g101635 49.551 557 264 7 2 553 8 552 0.0 567
MS.gene51086.t1 MTR_3g101640 47.931 580 280 8 1 567 1 571 0.0 562
MS.gene51086.t1 MTR_3g462760 48.913 552 261 8 20 567 22 556 0.0 556
MS.gene51086.t1 MTR_5g081810 50.654 535 243 6 37 567 12 529 0.0 553
MS.gene51086.t1 MTR_7g065970 49.050 579 274 9 1 567 1 570 0.0 545
MS.gene51086.t1 MTR_4g064530 51.233 527 241 9 12 531 32 549 0.0 538
MS.gene51086.t1 MTR_4g019225 48.921 556 261 8 25 567 27 572 0.0 534
MS.gene51086.t1 MTR_7g065980 45.724 573 285 10 10 567 10 571 0.0 532
MS.gene51086.t1 MTR_8g027375 44.615 585 292 6 1 567 1 571 1.28e-178 516
MS.gene51086.t1 MTR_8g091390 30.887 586 321 20 19 567 29 567 2.08e-71 239
MS.gene51086.t1 MTR_3g078730 29.525 569 346 13 7 564 8 532 4.99e-67 227
MS.gene51086.t1 MTR_3g078700 29.181 562 334 15 1 545 4 518 6.72e-66 224
MS.gene51086.t1 MTR_3g078740 31.122 588 333 20 21 564 23 582 2.16e-65 224
MS.gene51086.t1 MTR_5g081790 42.160 287 144 6 243 525 152 420 4.12e-65 219
MS.gene51086.t1 MTR_5g081790 48.795 166 75 2 8 163 5 170 2.93e-47 171
MS.gene51086.t1 MTR_8g479390 27.879 495 306 15 38 527 45 493 4.81e-42 159
MS.gene51086.t1 MTR_4g101650 28.839 534 334 18 7 527 9 509 1.21e-41 159
MS.gene51086.t1 MTR_3g008580 25.789 570 379 20 9 567 15 551 2.20e-39 152
MS.gene51086.t1 MTR_1g090707 26.519 543 342 13 38 567 38 536 3.67e-39 151
MS.gene51086.t1 MTR_4g049390 26.707 498 301 14 42 527 45 490 1.60e-37 146
MS.gene51086.t1 MTR_8g073840 39.572 187 74 4 17 202 23 171 2.84e-37 140
MS.gene51086.t1 MTR_8g479380 27.843 510 291 18 42 533 47 497 1.44e-36 143
MS.gene51086.t1 MTR_5g018270 27.327 505 293 17 38 527 45 490 2.75e-36 142
MS.gene51086.t1 MTR_1g026410 25.651 499 317 13 42 533 47 498 3.71e-36 142
MS.gene51086.t1 MTR_4g075690 24.823 564 373 15 8 567 16 532 5.87e-36 142
MS.gene51086.t1 MTR_3g115050 25.794 504 310 15 42 533 48 499 1.91e-35 140
MS.gene51086.t1 MTR_2g088080 26.531 490 312 12 42 527 48 493 1.39e-34 137
MS.gene51086.t1 MTR_4g048350 26.531 490 311 15 42 527 45 489 2.91e-34 137
MS.gene51086.t1 MTR_7g051440 26.820 522 327 17 27 532 32 514 1.51e-32 132
MS.gene51086.t1 MTR_3g083430 24.816 544 348 15 38 567 39 535 8.43e-32 129
MS.gene51086.t1 MTR_3g083390 24.632 544 349 15 38 567 39 535 1.29e-31 129
MS.gene51086.t1 MTR_3g083400 24.815 540 353 14 38 567 39 535 3.78e-31 127
MS.gene51086.t1 MTR_3g083450 24.632 544 349 15 38 567 40 536 4.10e-31 127
MS.gene51086.t1 MTR_3g083440 24.265 544 351 14 38 567 39 535 6.20e-31 127
MS.gene51086.t1 MTR_4g083210 24.767 537 344 16 11 533 7 497 1.63e-30 125
MS.gene51086.t1 MTR_7g058670 60.494 81 31 1 81 160 13 93 3.67e-30 114
MS.gene51086.t1 MTR_3g083410 24.627 536 351 14 42 567 7 499 5.21e-30 124
MS.gene51086.t1 MTR_4g075690 25.105 478 308 14 8 481 16 447 2.44e-29 121
MS.gene51086.t1 MTR_4g132320 25.989 531 335 20 7 527 12 494 3.23e-28 119
MS.gene51086.t1 MTR_4g083190 25.331 529 356 14 42 567 45 537 6.55e-28 118
MS.gene51086.t1 MTR_2g088080 25.055 451 290 12 81 527 6 412 3.87e-24 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51086.t1 AT2G40370 76.637 565 123 3 12 567 16 580 0.0 931
MS.gene51086.t1 AT5G05390 76.883 571 122 3 1 567 1 565 0.0 929
MS.gene51086.t1 AT2G30210 64.161 572 198 4 1 567 1 570 0.0 797
MS.gene51086.t1 AT5G07130 65.499 571 191 6 1 567 1 569 0.0 775
MS.gene51086.t1 AT5G07130 65.432 486 162 6 86 567 1 484 0.0 650
MS.gene51086.t1 AT5G07130 65.432 486 162 6 86 567 1 484 0.0 650
MS.gene51086.t1 AT5G09360 51.053 570 264 7 1 567 12 569 0.0 602
MS.gene51086.t1 AT2G29130 51.034 580 251 11 7 567 8 573 0.0 589
MS.gene51086.t1 AT5G60020 49.481 578 270 11 7 567 5 577 0.0 568
MS.gene51086.t1 AT5G03260 49.033 569 265 7 8 567 5 557 0.0 568
MS.gene51086.t1 AT2G38080 47.880 566 273 8 7 567 10 558 0.0 565
MS.gene51086.t1 AT5G60020 51.005 547 249 10 35 567 4 545 0.0 563
MS.gene51086.t1 AT3G09220 49.909 549 255 7 11 550 13 550 0.0 560
MS.gene51086.t1 AT5G01190 49.735 565 264 6 9 567 8 558 0.0 559
MS.gene51086.t1 AT5G58910 46.397 569 284 10 6 567 13 567 0.0 546
MS.gene51086.t1 AT5G58910 46.303 568 285 9 6 567 13 566 0.0 542
MS.gene51086.t1 AT5G01190 51.233 527 239 5 45 567 32 544 0.0 542
MS.gene51086.t1 AT5G48100 46.835 553 278 9 4 551 6 547 0.0 533
MS.gene51086.t1 AT2G46570 45.470 574 286 6 7 567 10 569 0.0 532
MS.gene51086.t1 AT1G18140 48.122 559 263 12 27 567 32 581 0.0 524
MS.gene51086.t1 AT5G01040 45.550 573 286 9 5 567 10 566 2.73e-180 521
MS.gene51086.t1 AT5G60020 49.254 536 250 11 7 525 5 535 8.20e-180 518
MS.gene51086.t1 AT5G58910 46.396 541 265 9 33 567 2 523 1.39e-179 517
MS.gene51086.t1 AT5G01050 45.739 575 284 8 5 567 10 568 4.38e-179 518
MS.gene51086.t1 AT2G46570 46.465 495 243 5 81 567 4 484 1.57e-163 474
MS.gene51086.t1 AT3G09220 50.431 464 210 7 96 550 1 453 8.48e-163 472
MS.gene51086.t1 AT5G21100 31.261 579 326 20 19 564 19 558 3.62e-73 244
MS.gene51086.t1 AT4G39830 30.757 608 339 22 1 567 11 577 3.06e-71 239
MS.gene51086.t1 AT5G21105 30.272 588 345 18 7 564 21 573 5.25e-69 233
MS.gene51086.t1 AT5G21105 29.690 549 325 17 7 525 21 538 3.52e-59 206
MS.gene51086.t1 AT4G39830 29.942 521 286 20 85 567 3 482 1.73e-51 184
MS.gene51086.t1 AT4G39830 29.808 520 286 20 86 567 2 480 9.41e-51 182
MS.gene51086.t1 AT4G39830 29.287 519 279 18 85 567 3 469 8.44e-49 176
MS.gene51086.t1 AT5G66920 26.829 533 334 16 42 567 55 538 1.70e-43 163
MS.gene51086.t1 AT4G12420 27.405 551 342 20 1 532 1 512 6.54e-43 162
MS.gene51086.t1 AT4G12420 27.405 551 342 20 1 532 1 512 6.54e-43 162
MS.gene51086.t1 AT1G75790 26.386 523 344 12 8 527 11 495 1.17e-41 158
MS.gene51086.t1 AT5G51480 26.241 564 369 16 17 567 21 550 6.73e-41 156
MS.gene51086.t1 AT1G21860 25.709 529 339 15 11 533 16 496 1.75e-38 149
MS.gene51086.t1 AT1G76160 24.621 528 355 14 7 533 11 496 4.66e-38 147
MS.gene51086.t1 AT5G21105 29.508 366 216 11 208 564 50 382 3.03e-36 140
MS.gene51086.t1 AT4G25240 26.389 576 372 18 8 567 11 550 4.29e-36 142
MS.gene51086.t1 AT4G25240 26.389 576 372 18 8 567 11 550 4.29e-36 142
MS.gene51086.t1 AT1G41830 25.948 501 312 18 42 533 47 497 2.90e-35 139
MS.gene51086.t1 AT1G21850 25.651 538 337 18 7 533 11 496 4.21e-35 139
MS.gene51086.t1 AT5G48450 25.045 559 373 17 9 564 13 528 6.44e-34 136
MS.gene51086.t1 AT5G48450 25.045 559 373 17 9 564 13 528 8.34e-34 136
MS.gene51086.t1 AT4G22010 26.923 494 306 17 42 527 44 490 5.24e-32 130
MS.gene51086.t1 AT4G37160 26.087 575 359 16 7 567 12 534 1.61e-30 125
MS.gene51086.t1 AT2G23630 27.052 536 330 17 42 567 53 537 1.63e-30 125
MS.gene51086.t1 AT2G23630 27.052 536 330 17 42 567 48 532 1.70e-30 125
MS.gene51086.t1 AT1G55570 25.761 493 320 12 42 527 47 500 4.46e-29 121
MS.gene51086.t1 AT3G13390 25.586 512 331 12 25 527 29 499 1.75e-28 120
MS.gene51086.t1 AT1G55560 24.813 536 334 18 42 564 44 523 1.15e-27 117
MS.gene51086.t1 AT4G28090 23.742 497 326 15 45 533 49 500 3.39e-27 116
MS.gene51086.t1 AT3G13400 25.251 499 314 15 42 527 45 497 5.90e-25 109
MS.gene51086.t1 AT3G13400 25.251 499 314 15 42 527 45 497 5.90e-25 109
MS.gene51086.t1 AT4G37160 29.747 316 204 5 7 319 12 312 2.97e-24 106
MS.gene51086.t1 AT4G38420 23.077 494 330 15 42 527 48 499 5.37e-22 100
MS.gene51086.t1 AT4G38420 26.971 241 166 5 42 281 48 279 1.57e-19 92.0
MS.gene51086.t1 AT4G38420 26.971 241 166 5 42 281 48 279 2.81e-19 91.7

Find 139 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAAAAGCTTTAGAAGTTTA+AGG 0.083519 5.1:-65688529 MS.gene51086:CDS
ACCTATAGATTTACCATTAA+TGG 0.101466 5.1:-65689921 MS.gene51086:CDS
CACCTACTACTGTTAGTTTA+TGG 0.135494 5.1:+65688831 None:intergenic
TGTACCTTCTTGTCCATTAA+TGG 0.180702 5.1:+65689908 None:intergenic
TGGCTTAGAGCCACAGTTTA+TGG 0.186697 5.1:-65689861 MS.gene51086:CDS
CGTGAAACACCCATTCTTCT+AGG 0.253813 5.1:-65689783 MS.gene51086:intron
ACAATTGTTCCAATTGATTC+AGG 0.259031 5.1:-65688920 MS.gene51086:CDS
TATATTGTTGCTGAGGGATT+TGG 0.270036 5.1:-65688107 MS.gene51086:CDS
ACTGTTGCTGTACCTGTTAA+TGG 0.271612 5.1:-65688023 MS.gene51086:CDS
TCTGATGCATACACTATTAA+TGG 0.273121 5.1:-65689360 MS.gene51086:CDS
CCAACTTTGGAAATTAATAA+TGG 0.283923 5.1:-65690795 MS.gene51086:CDS
AAAGTTACTAAGTTGAGATT+TGG 0.284792 5.1:-65688212 MS.gene51086:CDS
GGTGGCATGCTCATAGTTCA+TGG 0.302145 5.1:-65689881 MS.gene51086:CDS
TGAGGACCTGCCTTTGTGTT+AGG 0.313647 5.1:-65687342 None:intergenic
GTGCAGCATTGATTACTCTT+AGG 0.316195 5.1:+65688885 None:intergenic
GTGTATGCATCAGATATATT+TGG 0.328938 5.1:+65689369 None:intergenic
GGACATTGAGTCACAAATTC+TGG 0.334936 5.1:+65689966 None:intergenic
AGATCTTCATCAATTTCATT+AGG 0.335973 5.1:+65688494 None:intergenic
AGAGGATGGAGAAGGGCTAT+TGG 0.338124 5.1:-65687384 MS.gene51086:CDS
ACAACCAATGTTCTCATGTT+AGG 0.341290 5.1:-65688782 MS.gene51086:CDS
TGTTCACATTGGATGGGGTT+TGG 0.346069 5.1:-65687423 MS.gene51086:CDS
GTCCTCAGGAGGAGCTTCTA+TGG 0.350489 5.1:+65687358 None:intergenic
GCTTGTCTCACAACATCAAT+TGG 0.351287 5.1:+65689411 None:intergenic
AATCACCCTATTCATCTTCA+TGG 0.351556 5.1:-65688140 MS.gene51086:CDS
TCATATCCATGAAGATGAAT+AGG 0.353366 5.1:+65688134 None:intergenic
CCATTATTAATTTCCAAAGT+TGG 0.356421 5.1:+65690795 None:intergenic
GTTTGATGTATGTAGGTGTT+TGG 0.364623 5.1:-65687466 MS.gene51086:intron
TTTGTTTCTCCTGAATCAAT+TGG 0.365718 5.1:+65688911 None:intergenic
CGCCGGGCGCGCTTACCAAT+CGG 0.373787 5.1:-65688699 MS.gene51086:CDS
GGATAAGCATCTCCTTCCTT+AGG 0.376333 5.1:+65689822 None:intergenic
AATTAATAATGGAGATACTT+TGG 0.377624 5.1:-65690784 MS.gene51086:CDS
GAGATTTGGATCAAGGGTTC+AGG 0.378810 5.1:-65688198 MS.gene51086:CDS
ACAATTTCCTGGTCCAACTT+TGG 0.400549 5.1:-65690808 MS.gene51086:CDS
ATAACTGCCCAACCATTAAC+AGG 0.400604 5.1:+65688011 None:intergenic
AGAATGGGTGTTTCACGCTT+TGG 0.403573 5.1:+65689789 None:intergenic
CTAAGTTGAGATTTGGATCA+AGG 0.404145 5.1:-65688205 MS.gene51086:CDS
TGCTGTACCTGTTAATGGTT+GGG 0.408222 5.1:-65688018 MS.gene51086:CDS
TCCATTAATGGTAAATCTAT+AGG 0.411836 5.1:+65689920 None:intergenic
CAATGACCGACTCACATTAC+CGG 0.413180 5.1:+65688256 None:intergenic
AGCCGATTGGTAAGCGCGCC+CGG 0.414132 5.1:+65688697 None:intergenic
CCGGCGATGTAGTATCTTGA+TGG 0.420568 5.1:+65688716 None:intergenic
TCATCACCTTGTCCTGCAAA+AGG 0.422554 5.1:-65688623 MS.gene51086:CDS
GTTACAATGTCACCATTCAT+TGG 0.426411 5.1:-65690737 MS.gene51086:intron
ACTGTGAATGGACAATTTCC+TGG 0.436760 5.1:-65690819 MS.gene51086:CDS
ATCCTGCAGGCTCATAAACT+TGG 0.437751 5.1:-65688338 MS.gene51086:CDS
CTTGTCTCACAACATCAATT+GGG 0.442182 5.1:+65689412 None:intergenic
CTTGGATGTTCACATTGGAT+GGG 0.442663 5.1:-65687429 MS.gene51086:CDS
ATGATCATTACCTAGAAGAA+TGG 0.444961 5.1:+65689773 None:intergenic
TAACTTTCTCCAGGACGAAT+AGG 0.449717 5.1:+65689945 None:intergenic
ACTTGGATGTTCACATTGGA+TGG 0.450150 5.1:-65687430 MS.gene51086:CDS
GCAGGTAATGAAGGCATGAT+TGG 0.454006 5.1:+65688584 None:intergenic
CATATCCATGAAGATGAATA+GGG 0.455044 5.1:+65688135 None:intergenic
CATCACCTTGTCCTGCAAAA+GGG 0.460839 5.1:-65688622 MS.gene51086:CDS
GTGAGACAAGCCACACAAAC+AGG 0.461238 5.1:-65689399 MS.gene51086:CDS
GTGTTGCACAGCCTCTTCAC+TGG 0.461801 5.1:+65690855 None:intergenic
ATTATTATTAATTAGGGGAA+TGG 0.462012 5.1:-65689446 MS.gene51086:intron
TCATTGTGGCAACCGATTCA+AGG 0.473488 5.1:-65688239 MS.gene51086:CDS
TAACACAAAGGCAGGTCCTC+AGG 0.478994 5.1:+65687344 None:intergenic
ATTATTAATTAGGGGAATGG+TGG 0.479401 5.1:-65689443 MS.gene51086:intron
TTGCTGTACCTGTTAATGGT+TGG 0.479566 5.1:-65688019 MS.gene51086:CDS
CAAATGAGCATCACTGTGAA+TGG 0.479962 5.1:-65690831 MS.gene51086:CDS
TGATGATTTGTATTCAAGAA+TGG 0.480556 5.1:+65688640 None:intergenic
ATCAATGTCAAGGACCGAAC+GGG 0.480946 5.1:-65688418 MS.gene51086:CDS
GTAACTTTAGTCCCTTGAAT+CGG 0.482864 5.1:+65688227 None:intergenic
AGATTTGTTGCTGACAATCC+AGG 0.489827 5.1:-65687987 MS.gene51086:intron
AAACTCGTGCTCATGAACTT+TGG 0.490870 5.1:+65690996 None:intergenic
ACCTTGTTGATCCACCTATG+AGG 0.490977 5.1:-65688049 MS.gene51086:CDS
TTATTAATTAGGGGAATGGT+GGG 0.493359 5.1:-65689442 MS.gene51086:intron
ACAGCAACAGTGTTCCTCAT+AGG 0.495749 5.1:+65688035 None:intergenic
TTGGATGTTCACATTGGATG+GGG 0.496559 5.1:-65687428 MS.gene51086:CDS
TTGGATTATGCATTGTCACT+TGG 0.496947 5.1:-65687447 MS.gene51086:CDS
AACCATAAACTAACAGTAGT+AGG 0.497147 5.1:-65688833 MS.gene51086:CDS
GGGGTTAGACAAATCAGAAC+AGG 0.499855 5.1:-65690002 MS.gene51086:CDS
GATAAGCATCTCCTTCCTTA+GGG 0.506054 5.1:+65689823 None:intergenic
TGATCATTACCTAGAAGAAT+GGG 0.508193 5.1:+65689774 None:intergenic
ATGAGAACATTGGTTGTGAA+TGG 0.509668 5.1:+65688788 None:intergenic
TCACGCTTTGGCTTAGTGAA+AGG 0.510131 5.1:+65689801 None:intergenic
CTCCAAGTTTATGAGCCTGC+AGG 0.510477 5.1:+65688336 None:intergenic
GTGATATTATTACCAATGAA+TGG 0.515384 5.1:+65690725 None:intergenic
AGATGAATAGGGTGATTCTC+AGG 0.517564 5.1:+65688146 None:intergenic
ATCAGAACAGGATGGGCTGA+TGG 0.518148 5.1:-65689990 MS.gene51086:CDS
TTGATGGTGGTTGGTCACCT+TGG 0.518654 5.1:+65688732 None:intergenic
TTTGCAATTGTGAAGAAAAG+AGG 0.519484 5.1:+65688854 None:intergenic
ACTCAATGTCCTATTCGTCC+TGG 0.520865 5.1:-65689954 MS.gene51086:CDS
GATTATCCTAACACAAAGGC+AGG 0.522293 5.1:+65687336 None:intergenic
GCTCATAAACTTGGAGTACA+AGG 0.524236 5.1:-65688329 MS.gene51086:CDS
CAGCATTGATTACTCTTAGG+AGG 0.526575 5.1:+65688888 None:intergenic
GAAGTGAACCGTGTCCCGTT+CGG 0.526770 5.1:+65688404 None:intergenic
TTTACCATTAATGGACAAGA+AGG 0.529526 5.1:-65689912 MS.gene51086:CDS
GTGTTTGTGTCATTGTAAGC+AGG 0.530023 5.1:+65688566 None:intergenic
TGATTTGCATTAAAATTCGA+CGG 0.538672 5.1:+65688437 None:intergenic
TCATTGTAAGCAGGTAATGA+AGG 0.540128 5.1:+65688575 None:intergenic
TGATTTCTATATTGTTGCTG+AGG 0.540678 5.1:-65688114 MS.gene51086:CDS
CTTTACAAGTGCTCTACCAA+AGG 0.542011 5.1:-65689324 MS.gene51086:intron
TTTAATGCAAATCAATGTCA+AGG 0.544640 5.1:-65688428 MS.gene51086:CDS
CTATAGGTGTAACTTTCTCC+AGG 0.545148 5.1:+65689936 None:intergenic
TAAGTTGAGATTTGGATCAA+GGG 0.547755 5.1:-65688204 MS.gene51086:CDS
ACCACTGATGTTATAATCCA+AGG 0.548705 5.1:-65688749 MS.gene51086:CDS
CCATCAAGATACTACATCGC+CGG 0.549313 5.1:-65688716 MS.gene51086:CDS
GATTTCTATATTGTTGCTGA+GGG 0.549381 5.1:-65688113 MS.gene51086:CDS
TGGAGCTGCTCCTGTTTGTG+TGG 0.553190 5.1:+65689389 None:intergenic
CATTGTGGCAACCGATTCAA+GGG 0.556129 5.1:-65688238 MS.gene51086:CDS
ACCGGTGTAATCAAATTGCA+CGG 0.556156 5.1:+65688274 None:intergenic
AGCTCTAATCATTCACCCTA+AGG 0.560869 5.1:-65689838 MS.gene51086:CDS
CATCAAGATACTACATCGCC+GGG 0.561153 5.1:-65688715 MS.gene51086:CDS
ATCACCTTGTCCTGCAAAAG+GGG 0.563129 5.1:-65688621 MS.gene51086:CDS
TTAATTTCCAAAGTTGGACC+AGG 0.564251 5.1:+65690801 None:intergenic
GACAAGAAGGTACACTTTGG+TGG 0.574088 5.1:-65689899 MS.gene51086:CDS
TACACTATTAATGGTCAACC+TGG 0.583665 5.1:-65689351 MS.gene51086:CDS
GTCAAGGACCGAACGGGACA+CGG 0.584236 5.1:-65688412 MS.gene51086:CDS
CTAATCATTCACCCTAAGGA+AGG 0.586825 5.1:-65689834 MS.gene51086:CDS
TGTAGTATCTTGATGGTGGT+TGG 0.588008 5.1:+65688723 None:intergenic
ACCTTGGATTATAACATCAG+TGG 0.592085 5.1:+65688748 None:intergenic
GGTTGTGAATGGTTTGACAT+AGG 0.595120 5.1:+65688799 None:intergenic
ATGGACAAGAAGGTACACTT+TGG 0.605647 5.1:-65689902 MS.gene51086:CDS
TTTGTATTCAAGAATGGCAG+TGG 0.605780 5.1:+65688646 None:intergenic
ATTGTTGAGTCCTAATCCAA+TGG 0.608848 5.1:+65688463 None:intergenic
TAGACAAATCAGAACAGGAT+GGG 0.611575 5.1:-65689997 MS.gene51086:CDS
AATCAATGTCAAGGACCGAA+CGG 0.611682 5.1:-65688419 MS.gene51086:CDS
TACAAGCAACTCCAGTGAAG+AGG 0.611814 5.1:-65690866 MS.gene51086:CDS
ATAACATCAGTGGTTTGACC+AGG 0.615010 5.1:+65688758 None:intergenic
GAGCACTTGTAAAGATCACC+AGG 0.619435 5.1:+65689333 None:intergenic
GCAACAGTGTTCCTCATAGG+TGG 0.621280 5.1:+65688038 None:intergenic
TGTCACTTGGATGTTCACAT+TGG 0.623581 5.1:-65687434 MS.gene51086:CDS
GTGTAATCAAATTGCACGGG+AGG 0.625603 5.1:+65688278 None:intergenic
CCGGTGTAATCAAATTGCAC+GGG 0.626025 5.1:+65688275 None:intergenic
ATTACACCGGTAATGTGAGT+CGG 0.633330 5.1:-65688262 MS.gene51086:CDS
GTCCATAGAAGCTCCTCCTG+AGG 0.638740 5.1:-65687360 MS.gene51086:CDS
AATGTTCTCATGTTAGGACC+TGG 0.657280 5.1:-65688776 MS.gene51086:CDS
CACAAAGGCAGGTCCTCAGG+AGG 0.658358 5.1:+65687347 None:intergenic
TTTGGATTATCCTAACACAA+AGG 0.661322 5.1:+65687332 None:intergenic
GTAATGTGAGTCGGTCATTG+TGG 0.663037 5.1:-65688253 MS.gene51086:CDS
TCCTCATAGGTGGATCAACA+AGG 0.663984 5.1:+65688048 None:intergenic
TCACCTTGTCCTGCAAAAGG+GGG 0.666507 5.1:-65688620 MS.gene51086:CDS
CCCGTGCAATTTGATTACAC+CGG 0.670640 5.1:-65688275 MS.gene51086:CDS
TTAGACAAATCAGAACAGGA+TGG 0.677485 5.1:-65689998 MS.gene51086:CDS
AGGTCCTAACATGAGAACAT+TGG 0.691667 5.1:+65688778 None:intergenic
GATTAGAGCTCCATAAACTG+TGG 0.691733 5.1:+65689851 None:intergenic
GCGATGTAGTATCTTGATGG+TGG 0.696908 5.1:+65688719 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAATTATAAATTTTATA+GGG - chr5.1:65690677-65690696 MS.gene51086:intron 0.0%
!!! ATATTTTTTTTAAAAAAAAT+AGG + chr5.1:65687767-65687786 None:intergenic 0.0%
!! AAACTTTATCATAAAAATTA+AGG + chr5.1:65690720-65690739 None:intergenic 10.0%
!! ACTATAAAAATACTATTATA+TGG + chr5.1:65688200-65688219 None:intergenic 10.0%
!! ATGATGATTATTATTAATTA+GGG - chr5.1:65688948-65688967 MS.gene51086:intron 10.0%
!! ATTAACAACTAAAAATAAAA+AGG + chr5.1:65687459-65687478 None:intergenic 10.0%
!! CAATTAATTAATTATGTATA+TGG + chr5.1:65689305-65689324 None:intergenic 10.0%
!! TATGATGATTATTATTAATT+AGG - chr5.1:65688947-65688966 MS.gene51086:intron 10.0%
!! TTAACAACTAAAAATAAAAA+GGG + chr5.1:65687458-65687477 None:intergenic 10.0%
!!! ACATAAATTTTATTCAAAAT+TGG - chr5.1:65690543-65690562 MS.gene51086:intron 10.0%
!!! ATAATAGTATTTTTATAGTA+GGG - chr5.1:65688201-65688220 MS.gene51086:CDS 10.0%
!!! ATTTTGAATAAAATTTATGT+AGG + chr5.1:65690543-65690562 None:intergenic 10.0%
!!! TATAATAGTATTTTTATAGT+AGG - chr5.1:65688200-65688219 MS.gene51086:CDS 10.0%
!!! TTTTTTCAATTTGTTAATTA+AGG - chr5.1:65688813-65688832 MS.gene51086:CDS 10.0%
!! AAAACTAAAAAGTAACTATA+TGG + chr5.1:65688095-65688114 None:intergenic 15.0%
!! AGATAAGAATTAAAATAAGT+TGG + chr5.1:65688771-65688790 None:intergenic 15.0%
!! AGTGTAATAACAAATTTAAT+TGG - chr5.1:65687946-65687965 MS.gene51086:intron 15.0%
!! GGAGATTAATTATAATTAAT+TGG - chr5.1:65687489-65687508 MS.gene51086:intron 15.0%
!! TAAAAATACTATTATATGGA+AGG + chr5.1:65688196-65688215 None:intergenic 15.0%
!! TCAAAAATCAAAAACAAAAA+AGG + chr5.1:65688149-65688168 None:intergenic 15.0%
!! TGATGATTATTATTAATTAG+GGG - chr5.1:65688949-65688968 MS.gene51086:intron 15.0%
!! TGCAAATAATCAATAATTTA+TGG - chr5.1:65688342-65688361 MS.gene51086:CDS 15.0%
!! TTATATTCCTATTAGTATAT+AGG - chr5.1:65687872-65687891 MS.gene51086:intron 15.0%
!!! TAAGCAAAAATCAATATTTT+AGG + chr5.1:65689334-65689353 None:intergenic 15.0%
!! AATCTTTGTCTAAAAAGAAA+AGG + chr5.1:65688890-65688909 None:intergenic 20.0%
!! AATGATCATATGTCAAATAA+TGG + chr5.1:65689445-65689464 None:intergenic 20.0%
!! AATTATAATTAATCTCCCAA+AGG + chr5.1:65687486-65687505 None:intergenic 20.0%
!! ACATAAAATAATCAAACCTT+TGG + chr5.1:65689095-65689114 None:intergenic 20.0%
!! ATGCATGCTATAATTTATAA+AGG - chr5.1:65688309-65688328 MS.gene51086:CDS 20.0%
!! ATGTTTGATCTTATGTAATT+TGG - chr5.1:65690909-65690928 MS.gene51086:intron 20.0%
!! ATTATTATTAATTAGGGGAA+TGG - chr5.1:65688954-65688973 MS.gene51086:intron 20.0%
!! ATTATTTGACATATGATCAT+TGG - chr5.1:65689444-65689463 MS.gene51086:intron 20.0%
!! GTCTCAATAAAATCAAAATA+AGG - chr5.1:65690618-65690637 MS.gene51086:intron 20.0%
!! TAGTTGTTAATTTAACCTTT+GGG - chr5.1:65687468-65687487 MS.gene51086:intron 20.0%
!! TTAGTTGTTAATTTAACCTT+TGG - chr5.1:65687467-65687486 MS.gene51086:intron 20.0%
!! TTGAATAAAATTTATGTAGG+AGG + chr5.1:65690540-65690559 None:intergenic 20.0%
!!! AATTAATAATGGAGATACTT+TGG - chr5.1:65687616-65687635 MS.gene51086:intron 20.0%
!!! ACCACAATTTAAAATTTCAA+TGG - chr5.1:65688276-65688295 MS.gene51086:CDS 20.0%
!!! ACCATTGAAATTTTAAATTG+TGG + chr5.1:65688280-65688299 None:intergenic 20.0%
!!! ATTAGGGTTAATTATGTTTT+CGG - chr5.1:65690479-65690498 MS.gene51086:intron 20.0%
!!! TACTTTTCCTATATACTAAT+AGG + chr5.1:65687882-65687901 None:intergenic 20.0%
!!! TATATGGTCTTTTTAAATGT+CGG + chr5.1:65689289-65689308 None:intergenic 20.0%
!!! TTATTGCATTTTGAATCTTT+AGG - chr5.1:65688372-65688391 MS.gene51086:CDS 20.0%
!!! TTTTTGCTTATAATAGTGAT+CGG - chr5.1:65689344-65689363 MS.gene51086:CDS 20.0%
! AAAGTTTCACAAACATGATA+TGG - chr5.1:65690733-65690752 MS.gene51086:intron 25.0%
! AAGAAAAATTGCTAAGAAGA+AGG + chr5.1:65687362-65687381 None:intergenic 25.0%
! AAGGTGTAAGAAAACAAAAA+AGG + chr5.1:65688177-65688196 None:intergenic 25.0%
! AATGGAAAAATGTTTAGTCA+TGG + chr5.1:65689427-65689446 None:intergenic 25.0%
! ACCTATAGATTTACCATTAA+TGG - chr5.1:65688479-65688498 MS.gene51086:CDS 25.0%
! AGAATTCAATAAGTGATTGA+AGG + chr5.1:65689391-65689410 None:intergenic 25.0%
! AGATCTTCATCAATTTCATT+AGG + chr5.1:65689909-65689928 None:intergenic 25.0%
! ATAAATAGCAATGCTCAAAA+AGG - chr5.1:65688011-65688030 MS.gene51086:CDS 25.0%
! CCATTATTAATTTCCAAAGT+TGG + chr5.1:65687608-65687627 None:intergenic 25.0%
! GCATGTTTATTAATGTGAAA+AGG - chr5.1:65687511-65687530 MS.gene51086:intron 25.0%
! GGAAAAGTAAATGATAGATA+CGG - chr5.1:65687893-65687912 MS.gene51086:intron 25.0%
! GTGATATTATTACCAATGAA+TGG + chr5.1:65687678-65687697 None:intergenic 25.0%
! TAAATAGCAATGCTCAAAAA+GGG - chr5.1:65688012-65688031 MS.gene51086:CDS 25.0%
! TCCATTAATGGTAAATCTAT+AGG + chr5.1:65688483-65688502 None:intergenic 25.0%
! TTTAATGCAAATCAATGTCA+AGG - chr5.1:65689972-65689991 MS.gene51086:CDS 25.0%
!! AAAGTTACTAAGTTGAGATT+TGG - chr5.1:65690188-65690207 MS.gene51086:intron 25.0%
!! CCAACTTTGGAAATTAATAA+TGG - chr5.1:65687605-65687624 MS.gene51086:intron 25.0%
!! TGATGATTTGTATTCAAGAA+TGG + chr5.1:65689763-65689782 None:intergenic 25.0%
!! TGATTTGCATTAAAATTCGA+CGG + chr5.1:65689966-65689985 None:intergenic 25.0%
!!! AATTTTGAAGCATCTTTCTT+AGG + chr5.1:65690329-65690348 None:intergenic 25.0%
!!! ATTTTGAAGCATCTTTCTTA+GGG + chr5.1:65690328-65690347 None:intergenic 25.0%
!!! GTAAAAGCTTTAGAAGTTTA+AGG - chr5.1:65689871-65689890 MS.gene51086:CDS 25.0%
AAATGAGTTAAGACAAGTCA+GGG - chr5.1:65688063-65688082 MS.gene51086:CDS 30.0%
AACCATAAACTAACAGTAGT+AGG - chr5.1:65689567-65689586 MS.gene51086:intron 30.0%
ATCAAAATAAGGACTGAAAG+TGG - chr5.1:65690629-65690648 MS.gene51086:intron 30.0%
ATGATCATTACCTAGAAGAA+TGG + chr5.1:65688630-65688649 None:intergenic 30.0%
ATTATTAATTAGGGGAATGG+TGG - chr5.1:65688957-65688976 MS.gene51086:intron 30.0%
CATATCCATGAAGATGAATA+GGG + chr5.1:65690268-65690287 None:intergenic 30.0%
GACAAAGATTATGTTCATGA+TGG - chr5.1:65688900-65688919 MS.gene51086:CDS 30.0%
GATTTCTATATTGTTGCTGA+GGG - chr5.1:65690287-65690306 MS.gene51086:intron 30.0%
GTGTATGCATCAGATATATT+TGG + chr5.1:65689034-65689053 None:intergenic 30.0%
TAAATGAGTTAAGACAAGTC+AGG - chr5.1:65688062-65688081 MS.gene51086:CDS 30.0%
TCATATCCATGAAGATGAAT+AGG + chr5.1:65690269-65690288 None:intergenic 30.0%
TCTGATGCATACACTATTAA+TGG - chr5.1:65689040-65689059 MS.gene51086:intron 30.0%
TGATCATTACCTAGAAGAAT+GGG + chr5.1:65688629-65688648 None:intergenic 30.0%
TGATTTCTATATTGTTGCTG+AGG - chr5.1:65690286-65690305 MS.gene51086:intron 30.0%
TTATTAATTAGGGGAATGGT+GGG - chr5.1:65688958-65688977 MS.gene51086:intron 30.0%
TTTGCAATTGTGAAGAAAAG+AGG + chr5.1:65689549-65689568 None:intergenic 30.0%
TTTGGTTGTTTGATGTATGT+AGG - chr5.1:65690927-65690946 MS.gene51086:intron 30.0%
! ACAATTGTTCCAATTGATTC+AGG - chr5.1:65689480-65689499 MS.gene51086:intron 30.0%
! ATTTTGAATCTTTAGGCATG+GGG - chr5.1:65688379-65688398 MS.gene51086:CDS 30.0%
! CATTTTGAATCTTTAGGCAT+GGG - chr5.1:65688378-65688397 MS.gene51086:CDS 30.0%
! CTTAGCAATTTTTCTTCTCT+TGG - chr5.1:65687366-65687385 MS.gene51086:CDS 30.0%
! CTTTTCTTTACCATTGGATT+AGG - chr5.1:65689927-65689946 MS.gene51086:CDS 30.0%
! GAAGATCTTTTCTTTACCAT+TGG - chr5.1:65689921-65689940 MS.gene51086:CDS 30.0%
! TAAGTTGAGATTTGGATCAA+GGG - chr5.1:65690196-65690215 MS.gene51086:intron 30.0%
! TTTACCATTAATGGACAAGA+AGG - chr5.1:65688488-65688507 MS.gene51086:CDS 30.0%
! TTTGTTTCTCCTGAATCAAT+TGG + chr5.1:65689492-65689511 None:intergenic 30.0%
AAAAGGAATGTCACTCTACA+TGG + chr5.1:65688132-65688151 None:intergenic 35.0%
AATCACCCTATTCATCTTCA+TGG - chr5.1:65690260-65690279 MS.gene51086:intron 35.0%
ACAACCAATGTTCTCATGTT+AGG - chr5.1:65689618-65689637 MS.gene51086:intron 35.0%
ACCACTGATGTTATAATCCA+AGG - chr5.1:65689651-65689670 MS.gene51086:intron 35.0%
ACCTTGGATTATAACATCAG+TGG + chr5.1:65689655-65689674 None:intergenic 35.0%
ATTGTTGAGTCCTAATCCAA+TGG + chr5.1:65689940-65689959 None:intergenic 35.0%
CTTGTCTCACAACATCAATT+GGG + chr5.1:65688991-65689010 None:intergenic 35.0%
GAAATTGAGCTAATGTTACC+TGG + chr5.1:65690434-65690453 None:intergenic 35.0%
GAGACTAAGTACATGACTAA+AGG + chr5.1:65690603-65690622 None:intergenic 35.0%
GCAGTCAAATGAAATTGATG+TGG + chr5.1:65690804-65690823 None:intergenic 35.0%
GTAACTTTAGTCCCTTGAAT+CGG + chr5.1:65690176-65690195 None:intergenic 35.0%
GTTACAATGTCACCATTCAT+TGG - chr5.1:65687663-65687682 MS.gene51086:intron 35.0%
GTTTGATGTATGTAGGTGTT+TGG - chr5.1:65690934-65690953 MS.gene51086:intron 35.0%
TACACTATTAATGGTCAACC+TGG - chr5.1:65689049-65689068 MS.gene51086:intron 35.0%
TAGACAAATCAGAACAGGAT+GGG - chr5.1:65688403-65688422 MS.gene51086:CDS 35.0%
TATATTGTTGCTGAGGGATT+TGG - chr5.1:65690293-65690312 MS.gene51086:intron 35.0%
TCATTGTAAGCAGGTAATGA+AGG + chr5.1:65689828-65689847 None:intergenic 35.0%
TCTTAGTACAAGTGTGAAGA+AGG + chr5.1:65687830-65687849 None:intergenic 35.0%
TGCAGGATTGAAAAATTGCT+AGG + chr5.1:65690050-65690069 None:intergenic 35.0%
TGTACCTTCTTGTCCATTAA+TGG + chr5.1:65688495-65688514 None:intergenic 35.0%
TTAATTTCCAAAGTTGGACC+AGG + chr5.1:65687602-65687621 None:intergenic 35.0%
TTAGACAAATCAGAACAGGA+TGG - chr5.1:65688402-65688421 MS.gene51086:CDS 35.0%
TTGCTTATAATAGTGATCGG+AGG - chr5.1:65689347-65689366 MS.gene51086:CDS 35.0%
TTGGATTATGCATTGTCACT+TGG - chr5.1:65690953-65690972 MS.gene51086:intron 35.0%
TTTGTATTCAAGAATGGCAG+TGG + chr5.1:65689757-65689776 None:intergenic 35.0%
! ATGAGAACATTGGTTGTGAA+TGG + chr5.1:65689615-65689634 None:intergenic 35.0%
! CACCTACTACTGTTAGTTTA+TGG + chr5.1:65689572-65689591 None:intergenic 35.0%
! CTAAGTTGAGATTTGGATCA+AGG - chr5.1:65690195-65690214 MS.gene51086:intron 35.0%
! GATTATGTTCATGATGGAGA+TGG - chr5.1:65688906-65688925 MS.gene51086:CDS 35.0%
! GCATTTTGAATCTTTAGGCA+TGG - chr5.1:65688377-65688396 MS.gene51086:CDS 35.0%
! TCATGATGGAGATGGTTTAA+CGG - chr5.1:65688914-65688933 MS.gene51086:CDS 35.0%
!! AAAAAGTGTTTGCTTGCTTG+TGG + chr5.1:65687803-65687822 None:intergenic 35.0%
!! AAAAGTGTTTGCTTGCTTGT+GGG + chr5.1:65687802-65687821 None:intergenic 35.0%
!! AGTACAAGGTGTTTTTACGA+CGG - chr5.1:65690085-65690104 MS.gene51086:intron 35.0%
!! TAGCAATTTTTCAATCCTGC+AGG - chr5.1:65690049-65690068 MS.gene51086:intron 35.0%
AAACTCGTGCTCATGAACTT+TGG + chr5.1:65687407-65687426 None:intergenic 40.0%
AATCAATGTCAAGGACCGAA+CGG - chr5.1:65689981-65690000 MS.gene51086:CDS 40.0%
AATGTTCTCATGTTAGGACC+TGG - chr5.1:65689624-65689643 MS.gene51086:intron 40.0%
AATTGATGTGGCCTCAGTTA+TGG + chr5.1:65690792-65690811 None:intergenic 40.0%
ACAATTTCCTGGTCCAACTT+TGG - chr5.1:65687592-65687611 MS.gene51086:intron 40.0%
ACCGGTGTAATCAAATTGCA+CGG + chr5.1:65690129-65690148 None:intergenic 40.0%
ACTGTGAATGGACAATTTCC+TGG - chr5.1:65687581-65687600 MS.gene51086:intron 40.0%
ACTGTTGCTGTACCTGTTAA+TGG - chr5.1:65690377-65690396 MS.gene51086:intron 40.0%
ACTTGGATGTTCACATTGGA+TGG - chr5.1:65690970-65690989 MS.gene51086:intron 40.0%
AGATGAATAGGGTGATTCTC+AGG + chr5.1:65690257-65690276 None:intergenic 40.0%
AGGTCCTAACATGAGAACAT+TGG + chr5.1:65689625-65689644 None:intergenic 40.0%
ATAACATCAGTGGTTTGACC+AGG + chr5.1:65689645-65689664 None:intergenic 40.0%
ATAACTGCCCAACCATTAAC+AGG + chr5.1:65690392-65690411 None:intergenic 40.0%
ATTACACCGGTAATGTGAGT+CGG - chr5.1:65690138-65690157 MS.gene51086:intron 40.0%
CAAATGAGCATCACTGTGAA+TGG - chr5.1:65687569-65687588 MS.gene51086:intron 40.0%
CAGGACCAAGTATCTATATG+CGG - chr5.1:65690833-65690852 MS.gene51086:CDS 40.0%
CATATGATCATTGGCAATGC+AGG - chr5.1:65689453-65689472 MS.gene51086:intron 40.0%
CTAATCATTCACCCTAAGGA+AGG - chr5.1:65688566-65688585 MS.gene51086:CDS 40.0%
CTATAGGTGTAACTTTCTCC+AGG + chr5.1:65688467-65688486 None:intergenic 40.0%
CTTGGATGTTCACATTGGAT+GGG - chr5.1:65690971-65690990 MS.gene51086:intron 40.0%
CTTTACAAGTGCTCTACCAA+AGG - chr5.1:65689076-65689095 MS.gene51086:intron 40.0%
GATAAGCATCTCCTTCCTTA+GGG + chr5.1:65688580-65688599 None:intergenic 40.0%
GATTAGAGCTCCATAAACTG+TGG + chr5.1:65688552-65688571 None:intergenic 40.0%
GCGGTTGCGATATAAAACTT+TGG - chr5.1:65690852-65690871 MS.gene51086:CDS 40.0%
GCTCATAAACTTGGAGTACA+AGG - chr5.1:65690071-65690090 MS.gene51086:intron 40.0%
GCTTGTCTCACAACATCAAT+TGG + chr5.1:65688992-65689011 None:intergenic 40.0%
GGACATTGAGTCACAAATTC+TGG + chr5.1:65688437-65688456 None:intergenic 40.0%
GGTTGTGAATGGTTTGACAT+AGG + chr5.1:65689604-65689623 None:intergenic 40.0%
TAACTTTCTCCAGGACGAAT+AGG + chr5.1:65688458-65688477 None:intergenic 40.0%
TGCTGTACCTGTTAATGGTT+GGG - chr5.1:65690382-65690401 MS.gene51086:intron 40.0%
TTGCTGTACCTGTTAATGGT+TGG - chr5.1:65690381-65690400 MS.gene51086:intron 40.0%
TTGGATGTTCACATTGGATG+GGG - chr5.1:65690972-65690991 MS.gene51086:intron 40.0%
! AGCTCTAATCATTCACCCTA+AGG - chr5.1:65688562-65688581 MS.gene51086:CDS 40.0%
! ATGGACAAGAAGGTACACTT+TGG - chr5.1:65688498-65688517 MS.gene51086:CDS 40.0%
! GTGTTTGTGTCATTGTAAGC+AGG + chr5.1:65689837-65689856 None:intergenic 40.0%
! TGTCACTTGGATGTTCACAT+TGG - chr5.1:65690966-65690985 MS.gene51086:intron 40.0%
! TGTTTCTGTGACCATAACTG+AGG - chr5.1:65690778-65690797 MS.gene51086:CDS 40.0%
! TTTTGGTAGAGGATGGAGAA+GGG - chr5.1:65691009-65691028 MS.gene51086:CDS 40.0%
!! AGATTTGTTGCTGACAATCC+AGG - chr5.1:65690413-65690432 MS.gene51086:intron 40.0%
!! CAGCATTGATTACTCTTAGG+AGG + chr5.1:65689515-65689534 None:intergenic 40.0%
!! GTGCAGCATTGATTACTCTT+AGG + chr5.1:65689518-65689537 None:intergenic 40.0%
!! TGTAGTATCTTGATGGTGGT+TGG + chr5.1:65689680-65689699 None:intergenic 40.0%
!! TTTTTGGTAGAGGATGGAGA+AGG - chr5.1:65691008-65691027 MS.gene51086:CDS 40.0%
!!! ACTGTGTTTTTGGTAGAGGA+TGG - chr5.1:65691002-65691021 MS.gene51086:CDS 40.0%
ACAGCAACAGTGTTCCTCAT+AGG + chr5.1:65690368-65690387 None:intergenic 45.0%
ACCTTGTTGATCCACCTATG+AGG - chr5.1:65690351-65690370 MS.gene51086:intron 45.0%
ACTCAATGTCCTATTCGTCC+TGG - chr5.1:65688446-65688465 MS.gene51086:CDS 45.0%
AGAATGGGTGTTTCACGCTT+TGG + chr5.1:65688614-65688633 None:intergenic 45.0%
ATCAATGTCAAGGACCGAAC+GGG - chr5.1:65689982-65690001 MS.gene51086:CDS 45.0%
ATCACCTTGTCCTGCAAAAG+GGG - chr5.1:65689779-65689798 MS.gene51086:intron 45.0%
ATCCTGCAGGCTCATAAACT+TGG - chr5.1:65690062-65690081 MS.gene51086:intron 45.0%
ATTTGACTGCGATTCTCTGC+AGG - chr5.1:65690814-65690833 MS.gene51086:CDS 45.0%
CATCAAGATACTACATCGCC+GGG - chr5.1:65689685-65689704 MS.gene51086:intron 45.0%
CATCACCTTGTCCTGCAAAA+GGG - chr5.1:65689778-65689797 MS.gene51086:intron 45.0%
CCATCAAGATACTACATCGC+CGG - chr5.1:65689684-65689703 MS.gene51086:intron 45.0%
CCCGTGCAATTTGATTACAC+CGG - chr5.1:65690125-65690144 MS.gene51086:intron 45.0%
CCGGTGTAATCAAATTGCAC+GGG + chr5.1:65690128-65690147 None:intergenic 45.0%
CGCAACCGCATATAGATACT+TGG + chr5.1:65690841-65690860 None:intergenic 45.0%
CGTGAAACACCCATTCTTCT+AGG - chr5.1:65688617-65688636 MS.gene51086:CDS 45.0%
GAGATTTGGATCAAGGGTTC+AGG - chr5.1:65690202-65690221 MS.gene51086:intron 45.0%
GAGCACTTGTAAAGATCACC+AGG + chr5.1:65689070-65689089 None:intergenic 45.0%
GCGATGTAGTATCTTGATGG+TGG + chr5.1:65689684-65689703 None:intergenic 45.0%
GGATAAGCATCTCCTTCCTT+AGG + chr5.1:65688581-65688600 None:intergenic 45.0%
GGGGTTAGACAAATCAGAAC+AGG - chr5.1:65688398-65688417 MS.gene51086:CDS 45.0%
GTAATGTGAGTCGGTCATTG+TGG - chr5.1:65690147-65690166 MS.gene51086:intron 45.0%
GTGTAATCAAATTGCACGGG+AGG + chr5.1:65690125-65690144 None:intergenic 45.0%
TACAAGCAACTCCAGTGAAG+AGG - chr5.1:65687534-65687553 MS.gene51086:intron 45.0%
TCATCACCTTGTCCTGCAAA+AGG - chr5.1:65689777-65689796 MS.gene51086:intron 45.0%
TCCTCATAGGTGGATCAACA+AGG + chr5.1:65690355-65690374 None:intergenic 45.0%
TGGCTTAGAGCCACAGTTTA+TGG - chr5.1:65688539-65688558 MS.gene51086:CDS 45.0%
TGTTCACATTGGATGGGGTT+TGG - chr5.1:65690977-65690996 MS.gene51086:intron 45.0%
! AAATTGCACGGGAGGTTTTG+TGG + chr5.1:65690117-65690136 None:intergenic 45.0%
! ATATTTGCACCCCCTTTTGC+AGG + chr5.1:65689792-65689811 None:intergenic 45.0%
! CAATGACCGACTCACATTAC+CGG + chr5.1:65690147-65690166 None:intergenic 45.0%
! CATTGTGGCAACCGATTCAA+GGG - chr5.1:65690162-65690181 MS.gene51086:intron 45.0%
! GACAAGAAGGTACACTTTGG+TGG - chr5.1:65688501-65688520 MS.gene51086:CDS 45.0%
! GCAGGTAATGAAGGCATGAT+TGG + chr5.1:65689819-65689838 None:intergenic 45.0%
! GGGTTTGGCTACTGTGTTTT+TGG - chr5.1:65690992-65691011 MS.gene51086:intron 45.0%
! TCACGCTTTGGCTTAGTGAA+AGG + chr5.1:65688602-65688621 None:intergenic 45.0%
! TCATTGTGGCAACCGATTCA+AGG - chr5.1:65690161-65690180 MS.gene51086:intron 45.0%
!! AATTGCACGGGAGGTTTTGT+GGG + chr5.1:65690116-65690135 None:intergenic 45.0%
!!! GGCTACTGTGTTTTTGGTAG+AGG - chr5.1:65690998-65691017 MS.gene51086:CDS 45.0%
!! ATTGTATAAAAAATATAATT+AGG - chr5.1:65690462-65690481 MS.gene51086:intron 5.0%
!! TGTAAAAATTAATAAAAATA+GGG + chr5.1:65690570-65690589 None:intergenic 5.0%
!! TTGTAAAAATTAATAAAAAT+AGG + chr5.1:65690571-65690590 None:intergenic 5.0%
!! TTGTATAAAAAATATAATTA+GGG - chr5.1:65690463-65690482 MS.gene51086:intron 5.0%
!!! GAAAAAATTATAAATTTTAT+AGG - chr5.1:65690676-65690695 MS.gene51086:intron 5.0%
!!! TTTTATTAATTTTTACAAAA+TGG - chr5.1:65690573-65690592 MS.gene51086:intron 5.0%
AGAGGATGGAGAAGGGCTAT+TGG - chr5.1:65691016-65691035 MS.gene51086:CDS 50.0%
CCGGCGATGTAGTATCTTGA+TGG + chr5.1:65689687-65689706 None:intergenic 50.0%
CTCCAAGTTTATGAGCCTGC+AGG + chr5.1:65690067-65690086 None:intergenic 50.0%
GCAACAGTGTTCCTCATAGG+TGG + chr5.1:65690365-65690384 None:intergenic 50.0%
GGTGGCATGCTCATAGTTCA+TGG - chr5.1:65688519-65688538 MS.gene51086:CDS 50.0%
GTGAGACAAGCCACACAAAC+AGG - chr5.1:65689001-65689020 MS.gene51086:intron 50.0%
TAACACAAAGGCAGGTCCTC+AGG + chr5.1:65691059-65691078 None:intergenic 50.0%
TCACCTTGTCCTGCAAAAGG+GGG - chr5.1:65689780-65689799 MS.gene51086:intron 50.0%
! ACGCAGCATTTTGAGCCGAT+TGG + chr5.1:65689719-65689738 None:intergenic 50.0%
!! ATCAGAACAGGATGGGCTGA+TGG - chr5.1:65688410-65688429 MS.gene51086:CDS 50.0%
!! TTGATGGTGGTTGGTCACCT+TGG + chr5.1:65689671-65689690 None:intergenic 50.0%
GAAGTGAACCGTGTCCCGTT+CGG + chr5.1:65689999-65690018 None:intergenic 55.0%
GTCCATAGAAGCTCCTCCTG+AGG - chr5.1:65691040-65691059 MS.gene51086:CDS 55.0%
GTCCTCAGGAGGAGCTTCTA+TGG + chr5.1:65691045-65691064 None:intergenic 55.0%
GTGTTGCACAGCCTCTTCAC+TGG + chr5.1:65687548-65687567 None:intergenic 55.0%
TGGAGCTGCTCCTGTTTGTG+TGG + chr5.1:65689014-65689033 None:intergenic 55.0%
CACAAAGGCAGGTCCTCAGG+AGG + chr5.1:65691056-65691075 None:intergenic 60.0%
GTCAAGGACCGAACGGGACA+CGG - chr5.1:65689988-65690007 MS.gene51086:CDS 60.0%
! GCACCCCCTTTTGCAGGACA+AGG + chr5.1:65689786-65689805 None:intergenic 60.0%
AGCCGATTGGTAAGCGCGCC+CGG + chr5.1:65689706-65689725 None:intergenic 65.0%
CGCCGGGCGCGCTTACCAAT+CGG - chr5.1:65689701-65689720 MS.gene51086:intron 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 65687343 65691079 65687343 ID=MS.gene51086
chr5.1 mRNA 65687343 65691079 65687343 ID=MS.gene51086.t1;Parent=MS.gene51086
chr5.1 exon 65690993 65691079 65690993 ID=MS.gene51086.t1.exon1;Parent=MS.gene51086.t1
chr5.1 CDS 65690993 65691079 65690993 ID=cds.MS.gene51086.t1;Parent=MS.gene51086.t1
chr5.1 exon 65690738 65690889 65690738 ID=MS.gene51086.t1.exon2;Parent=MS.gene51086.t1
chr5.1 CDS 65690738 65690889 65690738 ID=cds.MS.gene51086.t1;Parent=MS.gene51086.t1
chr5.1 exon 65689784 65690028 65689784 ID=MS.gene51086.t1.exon3;Parent=MS.gene51086.t1
chr5.1 CDS 65689784 65690028 65689784 ID=cds.MS.gene51086.t1;Parent=MS.gene51086.t1
chr5.1 exon 65689325 65689453 65689325 ID=MS.gene51086.t1.exon4;Parent=MS.gene51086.t1
chr5.1 CDS 65689325 65689453 65689325 ID=cds.MS.gene51086.t1;Parent=MS.gene51086.t1
chr5.1 exon 65687988 65688947 65687988 ID=MS.gene51086.t1.exon5;Parent=MS.gene51086.t1
chr5.1 CDS 65687988 65688947 65687988 ID=cds.MS.gene51086.t1;Parent=MS.gene51086.t1
chr5.1 exon 65687343 65687473 65687343 ID=MS.gene51086.t1.exon6;Parent=MS.gene51086.t1
chr5.1 CDS 65687343 65687473 65687343 ID=cds.MS.gene51086.t1;Parent=MS.gene51086.t1
Gene Sequence

>MS.gene51086

ATGGAAGCTTTCAACACCTTCTTCTTAGCAATTTTTCTTCTCTTGGCTTCTTTAGCTAATGCCAAAGTTCATGAGCACGAGTTTGTCGTAAGTTTCTTTATTTTTTTTAAAGCCCTTTTTATTTTTAGTTGTTAATTTAACCTTTGGGAGATTAATTATAATTAATTGGCATGTTTATTAATGTGAAAAGGTACAAGCAACTCCAGTGAAGAGGCTGTGCAACACACAAATGAGCATCACTGTGAATGGACAATTTCCTGGTCCAACTTTGGAAATTAATAATGGAGATACTTTGGTTGTCAAAGTCATTAACAAAGCTCGTTACAATGTCACCATTCATTGGTAATAATATCACAAGCTAGCTAGAACTTTGTATATTAATTTTTATTTTATTTTGTTAACAAATTAATAGCTCACTTTGCCTATTTTTTTTAAAAAAAATATTAGATCACTTTACCCACAAGCAAGCAAACACTTTTTATATCCTTCTTCACACTTGTACTAAGAATTGATTGCAAGCTCACATTAATTATATTCCTATTAGTATATAGGAAAAGTAAATGATAGATACGGTGACAAAATTTATCAAGAAAATTTCAAGACAGTGTAATAACAAATTTAATTGGTTGAGACATTTTCTTGATAAATTCTTGATAAAAACTCTCTAGATAAATAGCAATGCTCAAAAAGGGAAATATATTAAGCATCATGATTGTGAATAAATGAGTTAAGACAAGTCAGGGTATATACCATATAGTTACTTTTTAGTTTTCTAAATATGATCAACCATGTAGAGTGACATTCCTTTTTTGTTTTTGATTTTTGAAATTTCCTTTTTTGTTTTCTTACACCTTCCATATAATAGTATTTTTATAGTAGGGTATGCAATTTGAGCTGTATATTTTTGAAAAAAAAAAGTCAAAATTATTTACAACCACAATTTAAAATTTCAATGGTATGTTTTTCATGCATGCTATAATTTATAAAGGATCATATATATGCAAATAATCAATAATTTATGGCTTGATTTTATTGCATTTTGAATCTTTAGGCATGGGGTTAGACAAATCAGAACAGGATGGGCTGATGGACCAGAATTTGTGACTCAATGTCCTATTCGTCCTGGAGAAAGTTACACCTATAGATTTACCATTAATGGACAAGAAGGTACACTTTGGTGGCATGCTCATAGTTCATGGCTTAGAGCCACAGTTTATGGAGCTCTAATCATTCACCCTAAGGAAGGAGATGCTTATCCTTTCACTAAGCCAAAGCGTGAAACACCCATTCTTCTAGGTAATGATCATTGTATTTGACATCTAACTTTTTTTTTAAGAACTTTGCAAACGTAATTATTAGATTTTTATATCTGATGTAACTGATGTGTGTAACATCGACTTGTAAAATGAAGAGTGTCATTCTTTACCAACTTATTTTAATTCTTATCTTTATTTAACGTGTGACTTAGATTTTTTTCAATTTGTTAATTAAGGATGTGATTGATATGAAATCATAAAGAAAATGAAATTATGAGATATGTTTTTCCTTTTCTTTTTAGACAAAGATTATGTTCATGATGGAGATGGTTTAACGGTTAAAATAATTATGATGATTATTATTAATTAGGGGAATGGTGGGATGCAAACCCAATTGATGTTGTGAGACAAGCCACACAAACAGGAGCAGCTCCAAATATATCTGATGCATACACTATTAATGGTCAACCTGGTGATCTTTACAAGTGCTCTACCAAAGGTTTGATTATTTTATGTTATTTTTTAATTTTGAGTAAATTTTTTAGTGAACTCATTATCAACATGTCACATATATGATTGATTAATTAATAAATTAAATAATTTTTTTATAAATTTACTATAGTCGAATATTTTATTTTAATTAGTTATAAGACAATCGTCAACAATTTTTCATAAAAAAAAAAGTAACCGACATTTAAAAAGACCATATACATAATTAATTAATTGAATGAACCTAAAATATTGATTTTTGCTTATAATAGTGATCGGAGGATGTATGCAATAACAACACCTTCAATCACTTATTGAATTCTTCATGATATATGACCATGACTAAACATTTTTCCATTATTTGACATATGATCATTGGCAATGCAGGCACAACAATTGTTCCAATTGATTCAGGAGAAACAAACCTCCTAAGAGTAATCAATGCTGCACTAAATCAACCTCTTTTCTTCACAATTGCAAACCATAAACTAACAGTAGTAGGTGCTGATGCTTCCTATGTCAAACCATTCACAACCAATGTTCTCATGTTAGGACCTGGTCAAACCACTGATGTTATAATCCAAGGTGACCAACCACCATCAAGATACTACATCGCCGGGCGCGCTTACCAATCGGCTCAAAATGCTGCGTTCGATAACACAACAACCACTGCCATTCTTGAATACAAATCATCACCTTGTCCTGCAAAAGGGGGTGCAAATATCAGACCAATCATGCCTTCATTACCTGCTTACAATGACACAAACACTGTTACATCATTTAGTAAAAGCTTTAGAAGTTTAAGGAATGTTGAAGTTCCTAATGAAATTGATGAAGATCTTTTCTTTACCATTGGATTAGGACTCAACAATTGTCCGTCGAATTTTAATGCAAATCAATGTCAAGGACCGAACGGGACACGGTTCACTTCTAGTATGAACAATGTGTCTTTTGTACTTCCTAGCAATTTTTCAATCCTGCAGGCTCATAAACTTGGAGTACAAGGTGTTTTTACGACGGATTTTCCCACAAAACCTCCCGTGCAATTTGATTACACCGGTAATGTGAGTCGGTCATTGTGGCAACCGATTCAAGGGACTAAAGTTACTAAGTTGAGATTTGGATCAAGGGTTCAGGTTGTTTTGCAAGATACTAGTATTGTTACTCCTGAGAATCACCCTATTCATCTTCATGGATATGATTTCTATATTGTTGCTGAGGGATTTGGAAATTATGACCCTAAGAAAGATGCTTCAAAATTTAACCTTGTTGATCCACCTATGAGGAACACTGTTGCTGTACCTGTTAATGGTTGGGCAGTTATCAGATTTGTTGCTGACAATCCAGGTAACATTAGCTCAATTTCAACTTTGCATTGTATAAAAAATATAATTAGGGTTAATTATGTTTTCGGTCTCTAAAAAATTAAGATTTTTTTTAAGTTTAATTCCTCCTACATAAATTTTATTCAAAATTGGTCCCTATTTTTATTAATTTTTACAAAATGGAGTACCTTTAGTCATGTACTTAGTCTCAATAAAATCAAAATAAGGACTGAAAGTGGACAATTTACGAAAAGATTAAACTCGAAAAAATTATAAATTTTATAGGGATTAAAAATATATTTAACCTTAATTTTTATGATAAAGTTTCACAAACATGATATGGATCGTGACATCAATGTCTTTGATGTTTCTGTGACCATAACTGAGGCCACATCAATTTCATTTGACTGCGATTCTCTGCAGGACCAAGTATCTATATGCGGTTGCGATATAAAACTTTGGTATAATGAAGAGAAAAAACATAATCAATTATTCAATGTTTGATCTTATGTAATTTGGTTGTTTGATGTATGTAGGTGTTTGGATTATGCATTGTCACTTGGATGTTCACATTGGATGGGGTTTGGCTACTGTGTTTTTGGTAGAGGATGGAGAAGGGCTATTGGAGTCCATAGAAGCTCCTCCTGAGGACCTGCCTTTGTGTTAG
Protein sequence

>MS.gene51086.t1

MEAFNTFFLAIFLLLASLANAKVHEHEFVVQATPVKRLCNTQMSITVNGQFPGPTLEINNGDTLVVKVINKARYNVTIHWHGVRQIRTGWADGPEFVTQCPIRPGESYTYRFTINGQEGTLWWHAHSSWLRATVYGALIIHPKEGDAYPFTKPKRETPILLGEWWDANPIDVVRQATQTGAAPNISDAYTINGQPGDLYKCSTKGTTIVPIDSGETNLLRVINAALNQPLFFTIANHKLTVVGADASYVKPFTTNVLMLGPGQTTDVIIQGDQPPSRYYIAGRAYQSAQNAAFDNTTTTAILEYKSSPCPAKGGANIRPIMPSLPAYNDTNTVTSFSKSFRSLRNVEVPNEIDEDLFFTIGLGLNNCPSNFNANQCQGPNGTRFTSSMNNVSFVLPSNFSILQAHKLGVQGVFTTDFPTKPPVQFDYTGNVSRSLWQPIQGTKVTKLRFGSRVQVVLQDTSIVTPENHPIHLHGYDFYIVAEGFGNYDPKKDASKFNLVDPPMRNTVAVPVNGWAVIRFVADNPGVWIMHCHLDVHIGWGLATVFLVEDGEGLLESIEAPPEDLPLC