Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51100.t1 | XP_003616734.2 | 98.7 | 76 | 1 | 0 | 1 | 76 | 1 | 76 | 2.30E-33 | 151 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51100.t1 | F4JZL7 | 44.3 | 61 | 34 | 0 | 7 | 67 | 14 | 74 | 4.1e-07 | 55.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51100.t1 | G7K7B2 | 98.7 | 76 | 1 | 0 | 1 | 76 | 1 | 76 | 1.6e-33 | 151.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052414 | MS.gene51100 | 0.847066 | 1.46E-59 | -1.69E-46 |
| MS.gene052999 | MS.gene51100 | 0.800425 | 1.45E-48 | -1.69E-46 |
| MS.gene053889 | MS.gene51100 | 0.80886 | 2.52E-50 | -1.69E-46 |
| MS.gene054556 | MS.gene51100 | 0.819206 | 1.31E-52 | -1.69E-46 |
| MS.gene055741 | MS.gene51100 | 0.800311 | 1.53E-48 | -1.69E-46 |
| MS.gene055744 | MS.gene51100 | 0.809237 | 2.09E-50 | -1.69E-46 |
| MS.gene055909 | MS.gene51100 | 0.821573 | 3.76E-53 | -1.69E-46 |
| MS.gene055911 | MS.gene51100 | 0.828743 | 7.59E-55 | -1.69E-46 |
| MS.gene057396 | MS.gene51100 | 0.80766 | 4.53E-50 | -1.69E-46 |
| MS.gene057749 | MS.gene51100 | 0.827902 | 1.21E-54 | -1.69E-46 |
| MS.gene057823 | MS.gene51100 | 0.831293 | 1.81E-55 | -1.69E-46 |
| MS.gene057826 | MS.gene51100 | 0.806 | 1.02E-49 | -1.69E-46 |
| MS.gene059420 | MS.gene51100 | 0.830045 | 3.66E-55 | -1.69E-46 |
| MS.gene059674 | MS.gene51100 | 0.802887 | 4.53E-49 | -1.69E-46 |
| MS.gene059807 | MS.gene51100 | 0.804556 | 2.04E-49 | -1.69E-46 |
| MS.gene059876 | MS.gene51100 | 0.82574 | 3.98E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51100.t1 | MTR_5g083710 | 98.684 | 76 | 1 | 0 | 1 | 76 | 1 | 76 | 3.71e-47 | 144 |
| MS.gene51100.t1 | MTR_3g072000 | 82.609 | 69 | 12 | 0 | 1 | 69 | 1 | 69 | 2.74e-26 | 91.7 |
| MS.gene51100.t1 | MTR_1g092670 | 32.836 | 67 | 45 | 0 | 2 | 68 | 3 | 69 | 2.69e-11 | 53.9 |
| MS.gene51100.t1 | MTR_5g055020 | 35.714 | 70 | 45 | 0 | 5 | 74 | 27 | 96 | 6.43e-11 | 54.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51100.t1 | AT5G05365 | 75.000 | 68 | 17 | 0 | 1 | 68 | 1 | 68 | 2.05e-30 | 102 |
| MS.gene51100.t1 | AT5G19090 | 44.262 | 61 | 34 | 0 | 7 | 67 | 14 | 74 | 3.20e-11 | 57.4 |
| MS.gene51100.t1 | AT5G19090 | 44.262 | 61 | 34 | 0 | 7 | 67 | 14 | 74 | 3.20e-11 | 57.4 |
| MS.gene51100.t1 | AT5G19090 | 44.262 | 61 | 34 | 0 | 7 | 67 | 14 | 74 | 3.20e-11 | 57.4 |
| MS.gene51100.t1 | AT5G19090 | 44.262 | 61 | 34 | 0 | 7 | 67 | 14 | 74 | 4.40e-11 | 57.0 |
| MS.gene51100.t1 | AT5G19090 | 44.262 | 61 | 34 | 0 | 7 | 67 | 14 | 74 | 4.40e-11 | 57.0 |
Find 12 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAACAAGGTCATCGTTAC+GGG | 0.390894 | 5.1:-65876683 | MS.gene51100:CDS |
| AACTCCAATCACTTCTTCAT+TGG | 0.396711 | 5.1:+65876652 | None:intergenic |
| ATTGGAGTTCTACATAAGAT+TGG | 0.430148 | 5.1:-65876638 | MS.gene51100:CDS |
| ATTGAACAAGGTCATCGTTA+CGG | 0.498697 | 5.1:-65876684 | MS.gene51100:CDS |
| AAGGCCATCAAGAAAATTGA+AGG | 0.519646 | 5.1:-65876973 | MS.gene51100:intron |
| GTCACCAATGAAGAAGTGAT+TGG | 0.524078 | 5.1:-65876656 | MS.gene51100:CDS |
| CAGGTGGTGGAAGTGAAGGT+TGG | 0.559119 | 5.1:-65877030 | MS.gene51100:CDS |
| TCATCAGGTGGTGGAAGTGA+AGG | 0.598746 | 5.1:-65877034 | MS.gene51100:intron |
| GTGTATCAAGAAAATTCTCA+AGG | 0.630976 | 5.1:-65876992 | MS.gene51100:CDS |
| TGTCGACAAGCAATTGAACA+AGG | 0.640540 | 5.1:-65876696 | MS.gene51100:CDS |
| TGGCAAGACTGCAACTGTGT+GGG | 0.657615 | 5.1:-65876618 | MS.gene51100:CDS |
| TTGGCAAGACTGCAACTGTG+TGG | 0.701929 | 5.1:-65876619 | MS.gene51100:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAAATACAAAAAAATAG+AGG | - | chr5.1:65876865-65876884 | MS.gene51100:intron | 10.0% |
| !!! | ATTATAGTAGAGTTTTGTAT+TGG | + | chr5.1:65876787-65876806 | None:intergenic | 20.0% |
| !!! | TATAATTTGTCTATAATGTG+TGG | - | chr5.1:65876801-65876820 | MS.gene51100:intron | 20.0% |
| !! | TTTTATTGTTATGTGTCATC+AGG | - | chr5.1:65876670-65876689 | MS.gene51100:CDS | 25.0% |
| !!! | AATAATTGTTGACTCATTTG+TGG | - | chr5.1:65876976-65876995 | MS.gene51100:CDS | 25.0% |
| ATTGGAGTTCTACATAAGAT+TGG | - | chr5.1:65877081-65877100 | MS.gene51100:intron | 30.0% | |
| GTGTATCAAGAAAATTCTCA+AGG | - | chr5.1:65876727-65876746 | MS.gene51100:intron | 30.0% | |
| ! | GTTACCTTCAATTTTCTTGA+TGG | + | chr5.1:65876753-65876772 | None:intergenic | 30.0% |
| !! | TTTTTGATCGACTCTTACAT+TGG | + | chr5.1:65876596-65876615 | None:intergenic | 30.0% |
| AACTCCAATCACTTCTTCAT+TGG | + | chr5.1:65877070-65877089 | None:intergenic | 35.0% | |
| AAGGCCATCAAGAAAATTGA+AGG | - | chr5.1:65876746-65876765 | MS.gene51100:intron | 35.0% | |
| ATTGAACAAGGTCATCGTTA+CGG | - | chr5.1:65877035-65877054 | MS.gene51100:intron | 35.0% | |
| TATTGTTATGTGTCATCAGG+TGG | - | chr5.1:65876673-65876692 | MS.gene51100:CDS | 35.0% | |
| GTCACCAATGAAGAAGTGAT+TGG | - | chr5.1:65877063-65877082 | MS.gene51100:intron | 40.0% | |
| TGTCGACAAGCAATTGAACA+AGG | - | chr5.1:65877023-65877042 | MS.gene51100:CDS | 40.0% | |
| TTGAACAAGGTCATCGTTAC+GGG | - | chr5.1:65877036-65877055 | MS.gene51100:intron | 40.0% | |
| TGTTATGTGTCATCAGGTGG+TGG | - | chr5.1:65876676-65876695 | MS.gene51100:CDS | 45.0% | |
| TGGCAAGACTGCAACTGTGT+GGG | - | chr5.1:65877101-65877120 | MS.gene51100:intron | 50.0% | |
| TTGGCAAGACTGCAACTGTG+TGG | - | chr5.1:65877100-65877119 | MS.gene51100:intron | 50.0% | |
| !! | TCATCAGGTGGTGGAAGTGA+AGG | - | chr5.1:65876685-65876704 | MS.gene51100:CDS | 50.0% |
| !! | CAGGTGGTGGAAGTGAAGGT+TGG | - | chr5.1:65876689-65876708 | MS.gene51100:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 65876589 | 65877152 | 65876589 | ID=MS.gene51100 |
| chr5.1 | mRNA | 65876589 | 65877152 | 65876589 | ID=MS.gene51100.t1;Parent=MS.gene51100 |
| chr5.1 | exon | 65877144 | 65877152 | 65877144 | ID=MS.gene51100.t1.exon1;Parent=MS.gene51100.t1 |
| chr5.1 | CDS | 65877144 | 65877152 | 65877144 | ID=cds.MS.gene51100.t1;Parent=MS.gene51100.t1 |
| chr5.1 | exon | 65876974 | 65877049 | 65876974 | ID=MS.gene51100.t1.exon2;Parent=MS.gene51100.t1 |
| chr5.1 | CDS | 65876974 | 65877049 | 65876974 | ID=cds.MS.gene51100.t1;Parent=MS.gene51100.t1 |
| chr5.1 | exon | 65876589 | 65876734 | 65876589 | ID=MS.gene51100.t1.exon3;Parent=MS.gene51100.t1 |
| chr5.1 | CDS | 65876589 | 65876734 | 65876589 | ID=cds.MS.gene51100.t1;Parent=MS.gene51100.t1 |
| Gene Sequence |
| Protein sequence |
