AlfalfaGEDB | MS.gene51597

M. sativa cultivar XinJiangDaYe / MS.gene51597

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51597.t1	AES80204.2	93	43	3	0	6	48	18	60	2.00E-10	75.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51597.t1	G7L4Q6	93.0	43	3	0	6	48	18	60	1.5e-10	75.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052447	MS.gene51597	0.859788	3.24E-63	-1.69E-46
MS.gene05329	MS.gene51597	0.816123	6.50E-52	-1.69E-46
MS.gene055952	MS.gene51597	0.811336	7.37E-51	-1.69E-46
MS.gene059458	MS.gene51597	0.80106	1.08E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51597.t1	MTR_7g077940	84.252	127	19	1	3	128	15	141	3.66e-28	100

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 16 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAAGCTGCTGCTGCATTTC+TGG	0.113322	7.2:-28043517	MS.gene51597:CDS
TGCTGCTGCTGAGGAAGTTG+TGG	0.454115	7.2:-28043655	MS.gene51597:CDS
CAGATGGTTGGTGTCGGAAT+GGG	0.477782	7.2:+28043751	None:intergenic
GAAAGCTGCTGCTGCAATTG+TGG	0.502554	7.2:-28043613	MS.gene51597:CDS
GCAGATGGTTGGTGTCGGAA+TGG	0.511338	7.2:+28043750	None:intergenic
TGCATTTCTTGCTGCTGCTG+AGG	0.512141	7.2:-28043472	MS.gene51597:CDS
GCATTTCTGGAAGACTCTGC+TGG	0.514359	7.2:-28043504	MS.gene51597:CDS
TGCAGTCTCCGTTGTGAAAG+CGG	0.521648	7.2:-28043436	MS.gene51597:CDS
GTCATTTCTTGCTGCTGCTG+AGG	0.534071	7.2:-28043664	MS.gene51597:CDS
TGAATTTCTTGCTGCTGCTG+AGG	0.554759	7.2:-28043568	MS.gene51597:CDS
GAAAACTGATGCAGCAATTG+TGG	0.561200	7.2:-28043709	MS.gene51597:CDS
CAGCAGCAGATGGTTGGTGT+CGG	0.596687	7.2:+28043745	None:intergenic
CAACTGCAGCAGCAGATGGT+TGG	0.625403	7.2:+28043739	None:intergenic
TGCAGTTGCCGCTTTCACAA+CGG	0.632718	7.2:+28043428	None:intergenic
ACTGCAACTGCAGCAGCAGA+TGG	0.634719	7.2:+28043735	None:intergenic
ATGGGTCAAGTTTCATCACA+AGG	0.648116	7.2:+28043769	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GGTTATATGATAATTATATA+TGG	+	chr7.2:28043419-28043438	None:intergenic	15.0%
!!	TACTTAGCTCATATAATTTA+TGG	+	chr7.2:28043460-28043479	None:intergenic	20.0%
	ATATAACCCAAACCTATCAC+TGG	-	chr7.2:28043431-28043450	MS.gene51597:CDS	35.0%
	TTTGTGACTTTGTCAATCCT+GGG	-	chr7.2:28043570-28043589	MS.gene51597:CDS	35.0%
!	TCATTTTCTAATTGCTTCCC+AGG	+	chr7.2:28043590-28043609	None:intergenic	35.0%
!	TTTTGTGACTTTGTCAATCC+TGG	-	chr7.2:28043569-28043588	MS.gene51597:CDS	35.0%
	AATTTATGGCAGCCAGTGAT+AGG	+	chr7.2:28043446-28043465	None:intergenic	40.0%
	ATGGGTCAAGTTTCATCACA+AGG	+	chr7.2:28043651-28043670	None:intergenic	40.0%
	CAAGGAGCTACAAACATTGA+TGG	+	chr7.2:28043633-28043652	None:intergenic	40.0%
	GAAAACTGATGCAGCAATTG+TGG	-	chr7.2:28043708-28043727	MS.gene51597:CDS	40.0%
	GAGCTACAAACATTGATGGA+AGG	+	chr7.2:28043629-28043648	None:intergenic	40.0%
	TGAATTTCTTGCTGCTGCTG+AGG	-	chr7.2:28043849-28043868	MS.gene51597:intron	45.0%
	ATGGCAGCCAGTGATAGGTT+TGG	+	chr7.2:28043441-28043460	None:intergenic	50.0%
	GAAAGCTGCTGCTGCAATTG+TGG	-	chr7.2:28043804-28043823	MS.gene51597:intron	50.0%
	GAAAGCTGCTGCTGCATTTC+TGG	-	chr7.2:28043900-28043919	MS.gene51597:intron	50.0%
	GTCATTTCTTGCTGCTGCTG+AGG	-	chr7.2:28043753-28043772	MS.gene51597:CDS	50.0%
	TGCAGTCTCCGTTGTGAAAG+CGG	-	chr7.2:28043981-28044000	MS.gene51597:intron	50.0%
	TGCATTTCTTGCTGCTGCTG+AGG	-	chr7.2:28043945-28043964	MS.gene51597:intron	50.0%
	TGGCAGCCAGTGATAGGTTT+GGG	+	chr7.2:28043440-28043459	None:intergenic	50.0%
!	GCATTTCTGGAAGACTCTGC+TGG	-	chr7.2:28043913-28043932	MS.gene51597:intron	50.0%
!!	CAGATGGTTGGTGTCGGAAT+GGG	+	chr7.2:28043669-28043688	None:intergenic	50.0%
!!	TGCAGTTGCCGCTTTCACAA+CGG	+	chr7.2:28043992-28044011	None:intergenic	50.0%
	ACTGCAACTGCAGCAGCAGA+TGG	+	chr7.2:28043685-28043704	None:intergenic	55.0%
	CAACTGCAGCAGCAGATGGT+TGG	+	chr7.2:28043681-28043700	None:intergenic	55.0%
	TGCTGCTGCTGAGGAAGTTG+TGG	-	chr7.2:28043762-28043781	MS.gene51597:CDS	55.0%
!	CAGCAGCAGATGGTTGGTGT+CGG	+	chr7.2:28043675-28043694	None:intergenic	55.0%
!!	GCAGATGGTTGGTGTCGGAA+TGG	+	chr7.2:28043670-28043689	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	28043407	28044032	28043407	ID=MS.gene51597
chr7.2	mRNA	28043407	28044032	28043407	ID=MS.gene51597.t1;Parent=MS.gene51597
chr7.2	exon	28044030	28044032	28044030	ID=MS.gene51597.t1.exon1;Parent=MS.gene51597.t1
chr7.2	CDS	28044030	28044032	28044030	ID=cds.MS.gene51597.t1;Parent=MS.gene51597.t1
chr7.2	exon	28043407	28043793	28043407	ID=MS.gene51597.t1.exon2;Parent=MS.gene51597.t1
chr7.2	CDS	28043407	28043793	28043407	ID=cds.MS.gene51597.t1;Parent=MS.gene51597.t1

Gene Sequence

>MS.gene51597

ATGGTAAGACCATATATAATTATCATATAACCCAAACCTATCACTGGCTGCCATAAATTATATGAGCTAAGTACTTGCGATTGCATGCTAGTAACATACAAATATAAGTTTAATGTTGAAACTGATATGCAAGAAAATGAGAAAATAAGCTAAGTACATGTGTTTTGTGACTTTGTCAATCCTGGGAAGCAATTAGAAAATGAGCTAACTCATGAAATTCCTTCCATCAATGTTTGTAGCTCCTTGTGATGAAACTTGACCCATTCCGACACCAACCATCTGCTGCTGCAGTTGCAGTTGTGAAAACTGATGCAGCAATTGTGGAAGATTCTGTTGATGTTGTTGTGTCATTTCTTGCTGCTGCTGAGGAAGTTGTGGATGTTGCAGTCGCAATTGTGAAAGCTGCTGCTGCAATTGTGGAAGATTCTGTTGATGTTGTTGTTGAATTTCTTGCTGCTGCTGAGGAAGTTGTTGACGCTGCAGTCGCAGTTGTGAAAGCTGCTGCTGCATTTCTGGAAGACTCTGCTGGTGTTGTTGCTGCATTTCTTGCTGCTGCTGAGGAAGTTGTTGAAGCTGCAGTCTCCGTTGTGAAAGCGGCAACTGCATTTGTGATGATTGTGACTTAA

Protein sequence

>MS.gene51597.t1

MLLVMKLDPFRHQPSAAAVAVVKTDAAIVEDSVDVVVSFLAAAEEVVDVAVAIVKAAAAIVEDSVDVVVEFLAAAEEVVDAAVAVVKAAAAFLEDSAGVVAAFLAAAEEVVEAAVSVVKAATAFVMIVT