AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene51841

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51841.t1 MTR_7g077840 96.964 527 16 0 1 527 1 527 0.0 1049
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51841.t1 AT4G29910 58.365 526 208 6 3 527 4 519 0.0 619
MS.gene51841.t1 AT4G29910 58.004 531 212 6 3 532 34 554 0.0 617

Find 103 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTTGAACGATGTATTTA+AGG 0.039494 7.4:+28417476 MS.gene51841:CDS
TTACTTCCTTTGTTTGTTTA+TGG 0.082867 7.4:+28416612 MS.gene51841:CDS
ATCTGAAGACTTGGTCTTAA+AGG 0.176570 7.4:+28418342 MS.gene51841:CDS
CTAAAAGGTCATTAATTGTT+AGG 0.230888 7.4:-28416485 None:intergenic
TCACGAGATTGTGCGTCTTT+TGG 0.252294 7.4:+28416572 MS.gene51841:CDS
GGTTTGCTTGGTTCCTCAAT+AGG 0.253121 7.4:-28417163 None:intergenic
ATAACCCATGTTAGTGTAAA+AGG 0.259909 7.4:-28417084 None:intergenic
AAAGACCTTGTCGTTCTTCT+TGG 0.270351 7.4:-28416402 None:intergenic
AAACAAACAAAGGAAGTAAT+GGG 0.347305 7.4:-28416608 None:intergenic
TCGTTCTTCTTGGGATTTGT+GGG 0.353908 7.4:-28416392 None:intergenic
TACCGAGTTGAGCCTTCAAT+TGG 0.365816 7.4:-28418307 None:intergenic
TGTGTAATCAGGAAAGTAAA+TGG 0.366600 7.4:-28417120 None:intergenic
GAAATGACGAATACAACTTC+TGG 0.370051 7.4:-28417184 None:intergenic
TAGAGTCTCCTCCAACTAAA+AGG 0.373158 7.4:-28416500 None:intergenic
CTCTCCAGTGGAAAAGTTCC+AGG 0.373590 7.4:-28418109 None:intergenic
AATTATTCAGACATCTCAAT+AGG 0.389184 7.4:+28416670 MS.gene51841:CDS
TGAATCTGACTACTTCTTCC+AGG 0.389815 7.4:-28416552 None:intergenic
GTCGTTCTTCTTGGGATTTG+TGG 0.391231 7.4:-28416393 None:intergenic
TCGATTATCAGAACCACCTT+TGG 0.394739 7.4:-28417691 None:intergenic
TTGTGAACCTCTAAGTGATA+AGG 0.402008 7.4:+28417383 MS.gene51841:CDS
TAAAAGGTCATTAATTGTTA+GGG 0.402778 7.4:-28416484 None:intergenic
CTCTTGTTTATTCTAGTTGT+AGG 0.407262 7.4:+28416694 MS.gene51841:CDS
AAGACCTTGTCGTTCTTCTT+GGG 0.408898 7.4:-28416401 None:intergenic
GAATAATTGAAGAATGATAC+TGG 0.409547 7.4:-28416655 None:intergenic
ATGTTAGTGTAAAAGGTATC+TGG 0.415813 7.4:-28417077 None:intergenic
ATATCACCTTCTGTGTAATC+AGG 0.421532 7.4:-28417131 None:intergenic
GTGTAATCAGGAAAGTAAAT+GGG 0.423617 7.4:-28417119 None:intergenic
CCTTCGAGTGAAGTTGAAAC+TGG 0.426012 7.4:+28417510 MS.gene51841:CDS
TTACACTAACATGGGTTATG+TGG 0.430020 7.4:+28417088 MS.gene51841:CDS
CTTCGCATTAGCATAACAAT+TGG 0.432380 7.4:-28416787 None:intergenic
TGTGAACCTCTAAGTGATAA+GGG 0.439039 7.4:+28417384 MS.gene51841:CDS
CATTAATTGTTAGGGATTCA+AGG 0.441406 7.4:-28416476 None:intergenic
TGTTTATGGAGGTGCTTCTA+CGG 0.442354 7.4:+28416626 MS.gene51841:CDS
TTTCTTCGTGAAACATTAAC+CGG 0.444332 7.4:+28416840 MS.gene51841:CDS
AGTGAAGCATCAAGAGTAGC+AGG 0.447937 7.4:-28417660 None:intergenic
TTTGTTCATCTTCATCAGCT+GGG 0.448183 7.4:-28418174 None:intergenic
AATACAACTTCTGGTTTGCT+TGG 0.449113 7.4:-28417175 None:intergenic
CTCCTCTTCATGTCTTCACT+AGG 0.450709 7.4:-28417420 None:intergenic
GGTAAAGAGACGAAGCATAA+GGG 0.458104 7.4:+28417531 MS.gene51841:CDS
CTTCCTTTGTTTGTTTATGG+AGG 0.462255 7.4:+28416615 MS.gene51841:CDS
ACAACCTTCAATATGATCTT+CGG 0.467205 7.4:+28418609 MS.gene51841:CDS
TAAGGGGAATCTGAAGAGAT+TGG 0.467367 7.4:+28417548 MS.gene51841:CDS
ATAACCGAAGATCATATTGA+AGG 0.469011 7.4:-28418613 None:intergenic
TGGTAAAGAGACGAAGCATA+AGG 0.472743 7.4:+28417530 MS.gene51841:CDS
AGCCTTTCTGTAGAATTACT+AGG 0.473672 7.4:+28417313 MS.gene51841:CDS
TAAACAAACAAAGGAAGTAA+TGG 0.475308 7.4:-28416609 None:intergenic
TGAAGGTTGTAGACCGCTCT+TGG 0.478034 7.4:-28418596 None:intergenic
CCTCTAAGTGATAAGGGAAA+AGG 0.486029 7.4:+28417390 MS.gene51841:CDS
GTTTATGGAGGTGCTTCTAC+GGG 0.488444 7.4:+28416627 MS.gene51841:CDS
TTGGAGAGATCTGAGGAAAT+CGG 0.495611 7.4:+28417567 MS.gene51841:CDS
CTGATAATCGAAAGCGAAAG+AGG 0.504756 7.4:+28417703 MS.gene51841:CDS
TACTTTCCTGATTACACAGA+AGG 0.511531 7.4:+28417125 MS.gene51841:CDS
ACTAAGATACATGGATAAAG+AGG 0.514425 7.4:+28416365 None:intergenic
ATGAAAATCAGACCAACCTC+AGG 0.521853 7.4:-28417041 None:intergenic
TGTAGAATTACTAGGCAGGT+TGG 0.526393 7.4:+28417321 MS.gene51841:CDS
TTTCTGTAGAATTACTAGGC+AGG 0.530292 7.4:+28417317 MS.gene51841:CDS
AAAGAAGGAAATGTTGGAAG+AGG 0.533397 7.4:+28418069 MS.gene51841:CDS
ACCGAGTTGAGCCTTCAATT+GGG 0.534244 7.4:-28418306 None:intergenic
AACTAGAAATTATATCATCT+AGG 0.541748 7.4:-28416527 None:intergenic
AGAAACAATGCACTGAAAGA+TGG 0.543949 7.4:-28418140 None:intergenic
TCAGGAAAGTAAATGGGGAT+AGG 0.544906 7.4:-28417113 None:intergenic
CTTCAGATTATTTATAACAC+CGG 0.547371 7.4:-28416859 None:intergenic
AAAATTAGCATTGCAGAGAG+TGG 0.552781 7.4:-28418263 None:intergenic
CGTTCTTCTTGGGATTTGTG+GGG 0.562446 7.4:-28416391 None:intergenic
ATCATAGAGATTAAACAGCA+AGG 0.565860 7.4:-28417006 None:intergenic
TCATAGAGATTAAACAGCAA+GGG 0.568496 7.4:-28417005 None:intergenic
CCAGTTTCAACTTCACTCGA+AGG 0.571131 7.4:-28417510 None:intergenic
CTAGGAGCTCAATGTGGTAG+CGG 0.571359 7.4:+28418208 MS.gene51841:CDS
ATATTCGTCAAATCCTATTG+AGG 0.571651 7.4:+28417150 MS.gene51841:CDS
AACAGGAAACATGATCTACT+TGG 0.572077 7.4:+28416929 MS.gene51841:CDS
TGCCTAGTAATTCTACAGAA+AGG 0.574180 7.4:-28417315 None:intergenic
GGTACAATATTTGAAGTGGA+TGG 0.578879 7.4:-28416432 None:intergenic
CTTAAATACATCGTTCAAAG+AGG 0.584918 7.4:-28417475 None:intergenic
ATCAACTATATCTGAAGACT+TGG 0.588459 7.4:+28418333 MS.gene51841:CDS
CGGTGTTATAAATAATCTGA+AGG 0.588859 7.4:+28416860 MS.gene51841:CDS
GAAGAGGAACTACTAATGAA+AGG 0.592132 7.4:+28418085 MS.gene51841:CDS
GAACAAAAGAATGAAGAACT+AGG 0.593521 7.4:+28418190 MS.gene51841:CDS
ATGCTAAAGATGCCTGAGGT+TGG 0.596497 7.4:+28417029 MS.gene51841:CDS
GCTGGAAAAGAAGGAAATGT+TGG 0.600775 7.4:+28418063 MS.gene51841:CDS
TCCCTAGTGAAGACATGAAG+AGG 0.602962 7.4:+28417418 MS.gene51841:CDS
AAATACATCGTTCAAAGAGG+AGG 0.606106 7.4:-28417472 None:intergenic
TCCTCTTCATGTCTTCACTA+GGG 0.609445 7.4:-28417419 None:intergenic
GTAAAGAGACGAAGCATAAG+GGG 0.615552 7.4:+28417532 MS.gene51841:CDS
CTACAACTAGAATAAACAAG+AGG 0.616716 7.4:-28416693 None:intergenic
GCCCAATTGAAGGCTCAACT+CGG 0.619028 7.4:+28418305 MS.gene51841:CDS
GGAAGTAATGGGGAATTCAA+CGG 0.622314 7.4:-28416597 None:intergenic
AAGAGTAGCAGGGTTTCTAG+AGG 0.622395 7.4:-28417649 None:intergenic
TGTAATCAGGAAAGTAAATG+GGG 0.623436 7.4:-28417118 None:intergenic
GCACCTCCATAAACAAACAA+AGG 0.623874 7.4:-28416618 None:intergenic
AGAAAATTGACAAAATCAGA+CGG 0.625993 7.4:-28416822 None:intergenic
GAAGAGATTGGAGAGATCTG+AGG 0.630497 7.4:+28417560 MS.gene51841:CDS
GAACTACTAATGAAAGGACC+TGG 0.635191 7.4:+28418091 MS.gene51841:CDS
TGCAGGTACAATATTTGAAG+TGG 0.636267 7.4:-28416436 None:intergenic
ATGGCTATCAACCTCTCCAG+TGG 0.639552 7.4:-28418121 None:intergenic
GTGAAGCATCAAGAGTAGCA+GGG 0.645856 7.4:-28417659 None:intergenic
TTCAAAGAGGAGGCAATATG+CGG 0.652269 7.4:-28417462 None:intergenic
CAGACTCGAACAAGATACGC+TGG 0.657694 7.4:-28416728 None:intergenic
CTAGTGAAGACATGAAGAGG+AGG 0.671922 7.4:+28417421 MS.gene51841:CDS
AACAAACAAAGGAAGTAATG+GGG 0.674348 7.4:-28416607 None:intergenic
TCTGATAGATCTACCAAGAG+CGG 0.694150 7.4:+28418583 MS.gene51841:intron
TGATATGCTAAAGATGCCTG+AGG 0.719075 7.4:+28417025 MS.gene51841:CDS
GAAGAACTAGGAGCTCAATG+TGG 0.747887 7.4:+28418202 MS.gene51841:CDS
AAATCCCAAGAAGAACGACA+AGG 0.747956 7.4:+28416397 MS.gene51841:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGCTAATTTTATTTTTAA+GGG + chr7.4:28418274-28418293 MS.gene51841:CDS 10.0%
!!! CAATGCTAATTTTATTTTTA+AGG + chr7.4:28418273-28418292 MS.gene51841:CDS 15.0%
!! AACTAGAAATTATATCATCT+AGG - chr7.4:28416530-28416549 None:intergenic 20.0%
!! AGAAAAATAAAACCAACAAA+AGG - chr7.4:28417828-28417847 None:intergenic 20.0%
!! TAAAAGGTCATTAATTGTTA+GGG - chr7.4:28416487-28416506 None:intergenic 20.0%
!! TGTATAAATACTTTGACAAT+GGG - chr7.4:28418507-28418526 None:intergenic 20.0%
!!! TCACAATATTTTTCAAATAG+TGG - chr7.4:28417369-28417388 None:intergenic 20.0%
! AAACAAACAAAGGAAGTAAT+GGG - chr7.4:28416611-28416630 None:intergenic 25.0%
! AATTATTCAGACATCTCAAT+AGG + chr7.4:28416670-28416689 MS.gene51841:CDS 25.0%
! AATTTCAACATTGTTTAAGC+AGG + chr7.4:28418462-28418481 MS.gene51841:intron 25.0%
! AGAAAATTGACAAAATCAGA+CGG - chr7.4:28416825-28416844 None:intergenic 25.0%
! AGATCATGTAATGCTTAAAA+TGG - chr7.4:28417248-28417267 None:intergenic 25.0%
! CTAAAAGGTCATTAATTGTT+AGG - chr7.4:28416488-28416507 None:intergenic 25.0%
! CTGTATAAATACTTTGACAA+TGG - chr7.4:28418508-28418527 None:intergenic 25.0%
! CTTCAGATTATTTATAACAC+CGG - chr7.4:28416862-28416881 None:intergenic 25.0%
! GAATAATTGAAGAATGATAC+TGG - chr7.4:28416658-28416677 None:intergenic 25.0%
! GTAGTTGATTGATAAGATTT+AGG + chr7.4:28417948-28417967 MS.gene51841:intron 25.0%
! TAAACAAACAAAGGAAGTAA+TGG - chr7.4:28416612-28416631 None:intergenic 25.0%
!! ACAATTAATGACCTTTTAGT+TGG + chr7.4:28416489-28416508 MS.gene51841:CDS 25.0%
!! ATTTTCATCTTGTTAGAGAT+TGG + chr7.4:28416961-28416980 MS.gene51841:CDS 25.0%
!! TTACTTCCTTTGTTTGTTTA+TGG + chr7.4:28416612-28416631 MS.gene51841:CDS 25.0%
!! TTTTCATCTTGTTAGAGATT+GGG + chr7.4:28416962-28416981 MS.gene51841:CDS 25.0%
!!! GATATAATTTCTAGTTTTCC+TGG + chr7.4:28416534-28416553 MS.gene51841:CDS 25.0%
AACAAACAAAGGAAGTAATG+GGG - chr7.4:28416610-28416629 None:intergenic 30.0%
ACAACCTTCAATATGATCTT+CGG + chr7.4:28418609-28418628 MS.gene51841:CDS 30.0%
ATAACCCATGTTAGTGTAAA+AGG - chr7.4:28417087-28417106 None:intergenic 30.0%
ATAACCGAAGATCATATTGA+AGG - chr7.4:28418616-28418635 None:intergenic 30.0%
ATATTCGTCAAATCCTATTG+AGG + chr7.4:28417150-28417169 MS.gene51841:CDS 30.0%
ATCAACTATATCTGAAGACT+TGG + chr7.4:28418333-28418352 MS.gene51841:CDS 30.0%
ATCATAGAGATTAAACAGCA+AGG - chr7.4:28417009-28417028 None:intergenic 30.0%
CAATAAATAACTCTTCTCAC+AGG - chr7.4:28417877-28417896 None:intergenic 30.0%
CATTAATTGTTAGGGATTCA+AGG - chr7.4:28416479-28416498 None:intergenic 30.0%
CTACAACTAGAATAAACAAG+AGG - chr7.4:28416696-28416715 None:intergenic 30.0%
CTCACAGGAAAAAATGAAAA+TGG - chr7.4:28417862-28417881 None:intergenic 30.0%
CTCTTTGAACGATGTATTTA+AGG + chr7.4:28417476-28417495 MS.gene51841:CDS 30.0%
CTTAAATACATCGTTCAAAG+AGG - chr7.4:28417478-28417497 None:intergenic 30.0%
GAACAAAAGAATGAAGAACT+AGG + chr7.4:28418190-28418209 MS.gene51841:CDS 30.0%
GCTCAATAAGAATGAACTAT+GGG - chr7.4:28417980-28417999 None:intergenic 30.0%
GTGTAATCAGGAAAGTAAAT+GGG - chr7.4:28417122-28417141 None:intergenic 30.0%
TCATAGAGATTAAACAGCAA+GGG - chr7.4:28417008-28417027 None:intergenic 30.0%
TGCTCAATAAGAATGAACTA+TGG - chr7.4:28417981-28418000 None:intergenic 30.0%
TGTAATCAGGAAAGTAAATG+GGG - chr7.4:28417121-28417140 None:intergenic 30.0%
TGTGTAATCAGGAAAGTAAA+TGG - chr7.4:28417123-28417142 None:intergenic 30.0%
TTTCTTCGTGAAACATTAAC+CGG + chr7.4:28416840-28416859 MS.gene51841:CDS 30.0%
! AGATACCTTTTACACTAACA+TGG + chr7.4:28417079-28417098 MS.gene51841:CDS 30.0%
! ATTTGAATGAAGCCTTTTGT+TGG + chr7.4:28417813-28417832 MS.gene51841:intron 30.0%
! CTCTTGTTTATTCTAGTTGT+AGG + chr7.4:28416694-28416713 MS.gene51841:CDS 30.0%
! GATACCTTTTACACTAACAT+GGG + chr7.4:28417080-28417099 MS.gene51841:CDS 30.0%
! GTTTTCAGCACATCAAAAAA+TGG - chr7.4:28417751-28417770 None:intergenic 30.0%
!! ATATGCTTTTTGATGCATAC+AGG + chr7.4:28418026-28418045 MS.gene51841:intron 30.0%
!! ATGTTAGTGTAAAAGGTATC+TGG - chr7.4:28417080-28417099 None:intergenic 30.0%
!! ATTAATGACCTTTTAGTTGG+AGG + chr7.4:28416492-28416511 MS.gene51841:CDS 30.0%
!! CGGTGTTATAAATAATCTGA+AGG + chr7.4:28416860-28416879 MS.gene51841:CDS 30.0%
!! TATGCTTTTTGATGCATACA+GGG + chr7.4:28418027-28418046 MS.gene51841:intron 30.0%
!!! AATTGGCAGCATTTTTTCTA+TGG - chr7.4:28416773-28416792 None:intergenic 30.0%
AAAAAGAAGATACATGGTGG+GGG + chr7.4:28416906-28416925 MS.gene51841:CDS 35.0%
AAAATTAGCATTGCAGAGAG+TGG - chr7.4:28418266-28418285 None:intergenic 35.0%
AAACAAAGCCATTCAGACAA+TGG - chr7.4:28417909-28417928 None:intergenic 35.0%
AAAGAAGGAAATGTTGGAAG+AGG + chr7.4:28418069-28418088 MS.gene51841:CDS 35.0%
AAATACATCGTTCAAAGAGG+AGG - chr7.4:28417475-28417494 None:intergenic 35.0%
AACAGGAAACATGATCTACT+TGG + chr7.4:28416929-28416948 MS.gene51841:CDS 35.0%
ACTTTGACAATGGGAACTTA+AGG - chr7.4:28418498-28418517 None:intergenic 35.0%
AGAAACAATGCACTGAAAGA+TGG - chr7.4:28418143-28418162 None:intergenic 35.0%
AGCCTTTCTGTAGAATTACT+AGG + chr7.4:28417313-28417332 MS.gene51841:CDS 35.0%
ATATCACCTTCTGTGTAATC+AGG - chr7.4:28417134-28417153 None:intergenic 35.0%
ATCCCGAAAAAGAAGATACA+TGG + chr7.4:28416900-28416919 MS.gene51841:CDS 35.0%
CGAAAAAGAAGATACATGGT+GGG + chr7.4:28416904-28416923 MS.gene51841:CDS 35.0%
CTTCGCATTAGCATAACAAT+TGG - chr7.4:28416790-28416809 None:intergenic 35.0%
GAAAAAGAAGATACATGGTG+GGG + chr7.4:28416905-28416924 MS.gene51841:CDS 35.0%
GAAATGACGAATACAACTTC+TGG - chr7.4:28417187-28417206 None:intergenic 35.0%
GAAGAGGAACTACTAATGAA+AGG + chr7.4:28418085-28418104 MS.gene51841:CDS 35.0%
TACTTTCCTGATTACACAGA+AGG + chr7.4:28417125-28417144 MS.gene51841:CDS 35.0%
TGCAGGTACAATATTTGAAG+TGG - chr7.4:28416439-28416458 None:intergenic 35.0%
TGCCTAGTAATTCTACAGAA+AGG - chr7.4:28417318-28417337 None:intergenic 35.0%
TGTGAACCTCTAAGTGATAA+GGG + chr7.4:28417384-28417403 MS.gene51841:CDS 35.0%
TTGTGAACCTCTAAGTGATA+AGG + chr7.4:28417383-28417402 MS.gene51841:CDS 35.0%
TTTCTGTAGAATTACTAGGC+AGG + chr7.4:28417317-28417336 MS.gene51841:CDS 35.0%
TTTGTTCATCTTCATCAGCT+GGG - chr7.4:28418177-28418196 None:intergenic 35.0%
! CACCATGTATCTTCTTTTTC+GGG - chr7.4:28416905-28416924 None:intergenic 35.0%
! CCACCATGTATCTTCTTTTT+CGG - chr7.4:28416906-28416925 None:intergenic 35.0%
! CTTCCTTTGTTTGTTTATGG+AGG + chr7.4:28416615-28416634 MS.gene51841:CDS 35.0%
! GGTACAATATTTGAAGTGGA+TGG - chr7.4:28416435-28416454 None:intergenic 35.0%
! TTACACTAACATGGGTTATG+TGG + chr7.4:28417088-28417107 MS.gene51841:CDS 35.0%
! TTTTGTTCATCTTCATCAGC+TGG - chr7.4:28418178-28418197 None:intergenic 35.0%
!! AATACAACTTCTGGTTTGCT+TGG - chr7.4:28417178-28417197 None:intergenic 35.0%
!! ATCTGAAGACTTGGTCTTAA+AGG + chr7.4:28418342-28418361 MS.gene51841:CDS 35.0%
!!! GCTTCACTTTTTGATTCCAA+AGG + chr7.4:28417675-28417694 MS.gene51841:CDS 35.0%
!!! TCACTTTTTGATTCCAAAGG+TGG + chr7.4:28417678-28417697 MS.gene51841:CDS 35.0%
AAAGACCTTGTCGTTCTTCT+TGG - chr7.4:28416405-28416424 None:intergenic 40.0%
AAAGCCATTCAGACAATGGT+TGG - chr7.4:28417905-28417924 None:intergenic 40.0%
AAATCCCAAGAAGAACGACA+AGG + chr7.4:28416397-28416416 MS.gene51841:CDS 40.0%
AAGACCTTGTCGTTCTTCTT+GGG - chr7.4:28416404-28416423 None:intergenic 40.0%
AGCGAAAGAGGAAGTAAGTA+TGG + chr7.4:28417715-28417734 MS.gene51841:intron 40.0%
AGTTCCAACCATTGTCTGAA+TGG + chr7.4:28417898-28417917 MS.gene51841:intron 40.0%
ATGAAAATCAGACCAACCTC+AGG - chr7.4:28417044-28417063 None:intergenic 40.0%
CATTGTTTAAGCAGGTTGCA+AGG + chr7.4:28418470-28418489 MS.gene51841:intron 40.0%
CCGAAAAAGAAGATACATGG+TGG + chr7.4:28416903-28416922 MS.gene51841:CDS 40.0%
CCTCTAAGTGATAAGGGAAA+AGG + chr7.4:28417390-28417409 MS.gene51841:CDS 40.0%
CTCGCGTACATGTGTAATAA+AGG + chr7.4:28418434-28418453 MS.gene51841:intron 40.0%
CTGATAATCGAAAGCGAAAG+AGG + chr7.4:28417703-28417722 MS.gene51841:CDS 40.0%
CTTTAACAATGACAGCTTCG+CGG + chr7.4:28418539-28418558 MS.gene51841:intron 40.0%
GAACTACTAATGAAAGGACC+TGG + chr7.4:28418091-28418110 MS.gene51841:CDS 40.0%
GCACCTCCATAAACAAACAA+AGG - chr7.4:28416621-28416640 None:intergenic 40.0%
GCTGGAAAAGAAGGAAATGT+TGG + chr7.4:28418063-28418082 MS.gene51841:CDS 40.0%
GGTAAAGAGACGAAGCATAA+GGG + chr7.4:28417531-28417550 MS.gene51841:CDS 40.0%
GTAAAGAGACGAAGCATAAG+GGG + chr7.4:28417532-28417551 MS.gene51841:CDS 40.0%
TAAGGGGAATCTGAAGAGAT+TGG + chr7.4:28417548-28417567 MS.gene51841:CDS 40.0%
TAGAGTCTCCTCCAACTAAA+AGG - chr7.4:28416503-28416522 None:intergenic 40.0%
TCAGGAAAGTAAATGGGGAT+AGG - chr7.4:28417116-28417135 None:intergenic 40.0%
TCCTCTTCATGTCTTCACTA+GGG - chr7.4:28417422-28417441 None:intergenic 40.0%
TCGATTATCAGAACCACCTT+TGG - chr7.4:28417694-28417713 None:intergenic 40.0%
TCGTTCTTCTTGGGATTTGT+GGG - chr7.4:28416395-28416414 None:intergenic 40.0%
TCTGATAGATCTACCAAGAG+CGG + chr7.4:28418583-28418602 MS.gene51841:intron 40.0%
TGAATCTGACTACTTCTTCC+AGG - chr7.4:28416555-28416574 None:intergenic 40.0%
TGATATGCTAAAGATGCCTG+AGG + chr7.4:28417025-28417044 MS.gene51841:CDS 40.0%
TGGTAAAGAGACGAAGCATA+AGG + chr7.4:28417530-28417549 MS.gene51841:CDS 40.0%
TGTAGAATTACTAGGCAGGT+TGG + chr7.4:28417321-28417340 MS.gene51841:CDS 40.0%
TTCAAAGAGGAGGCAATATG+CGG - chr7.4:28417465-28417484 None:intergenic 40.0%
TTGGAGAGATCTGAGGAAAT+CGG + chr7.4:28417567-28417586 MS.gene51841:CDS 40.0%
! CCTTTTCCCTTATCACTTAG+AGG - chr7.4:28417393-28417412 None:intergenic 40.0%
! GGAAGTAATGGGGAATTCAA+CGG - chr7.4:28416600-28416619 None:intergenic 40.0%
!! TGAAAAAGCGCTGGAAAAGA+AGG + chr7.4:28418054-28418073 MS.gene51841:CDS 40.0%
!! TGTTTATGGAGGTGCTTCTA+CGG + chr7.4:28416626-28416645 MS.gene51841:CDS 40.0%
ATGCTAAAGATGCCTGAGGT+TGG + chr7.4:28417029-28417048 MS.gene51841:CDS 45.0%
CCAGTTTCAACTTCACTCGA+AGG - chr7.4:28417513-28417532 None:intergenic 45.0%
CCTTCGAGTGAAGTTGAAAC+TGG + chr7.4:28417510-28417529 MS.gene51841:CDS 45.0%
CGCGAAGCTGTCATTGTTAA+AGG - chr7.4:28418541-28418560 None:intergenic 45.0%
CGTTCTTCTTGGGATTTGTG+GGG - chr7.4:28416394-28416413 None:intergenic 45.0%
CTAGTGAAGACATGAAGAGG+AGG + chr7.4:28417421-28417440 MS.gene51841:CDS 45.0%
CTCCTCTTCATGTCTTCACT+AGG - chr7.4:28417423-28417442 None:intergenic 45.0%
GAAGAACTAGGAGCTCAATG+TGG + chr7.4:28418202-28418221 MS.gene51841:CDS 45.0%
GAAGAGATTGGAGAGATCTG+AGG + chr7.4:28417560-28417579 MS.gene51841:CDS 45.0%
GGAAAAAGCTGCCCAATTGA+AGG + chr7.4:28418295-28418314 MS.gene51841:CDS 45.0%
GTCGTTCTTCTTGGGATTTG+TGG - chr7.4:28416396-28416415 None:intergenic 45.0%
TCCCTAGTGAAGACATGAAG+AGG + chr7.4:28417418-28417437 MS.gene51841:CDS 45.0%
! AAGAGTAGCAGGGTTTCTAG+AGG - chr7.4:28417652-28417671 None:intergenic 45.0%
! ACCGAGTTGAGCCTTCAATT+GGG - chr7.4:28418309-28418328 None:intergenic 45.0%
! AGTGAAGCATCAAGAGTAGC+AGG - chr7.4:28417663-28417682 None:intergenic 45.0%
! CACGAGATTGTGCGTCTTTT+GGG + chr7.4:28416573-28416592 MS.gene51841:CDS 45.0%
! GGTTTGCTTGGTTCCTCAAT+AGG - chr7.4:28417166-28417185 None:intergenic 45.0%
! GTGAAGCATCAAGAGTAGCA+GGG - chr7.4:28417662-28417681 None:intergenic 45.0%
! TACCGAGTTGAGCCTTCAAT+TGG - chr7.4:28418310-28418329 None:intergenic 45.0%
! TCACGAGATTGTGCGTCTTT+TGG + chr7.4:28416572-28416591 MS.gene51841:CDS 45.0%
!! CTGCTGTTTACTTTTGCTGC+AGG - chr7.4:28416456-28416475 None:intergenic 45.0%
!! GTTTATGGAGGTGCTTCTAC+GGG + chr7.4:28416627-28416646 MS.gene51841:CDS 45.0%
!!! CTTTTCCAGCGCTTTTTCAG+TGG - chr7.4:28418053-28418072 None:intergenic 45.0%
ATGGCTATCAACCTCTCCAG+TGG - chr7.4:28418124-28418143 None:intergenic 50.0%
CAGACTCGAACAAGATACGC+TGG - chr7.4:28416731-28416750 None:intergenic 50.0%
CTAGGAGCTCAATGTGGTAG+CGG + chr7.4:28418208-28418227 MS.gene51841:CDS 50.0%
CTCTCCAGTGGAAAAGTTCC+AGG - chr7.4:28418112-28418131 None:intergenic 50.0%
GCCCAATTGAAGGCTCAACT+CGG + chr7.4:28418305-28418324 MS.gene51841:CDS 50.0%
TGAAGGTTGTAGACCGCTCT+TGG - chr7.4:28418599-28418618 None:intergenic 50.0%
! AAGATACATGGTGGGGGAAC+AGG + chr7.4:28416912-28416931 MS.gene51841:CDS 50.0%
! AGGACCTGGAACTTTTCCAC+TGG + chr7.4:28418105-28418124 MS.gene51841:CDS 50.0%
! CTGGAACTTTTCCACTGGAG+AGG + chr7.4:28418110-28418129 MS.gene51841:CDS 50.0%
AATGACAGCTTCGCGGAGCT+TGG + chr7.4:28418546-28418565 MS.gene51841:intron 55.0%
! CAGGGCCACTGAAAAAGCGC+TGG + chr7.4:28418045-28418064 MS.gene51841:intron 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 28416375 28418638 28416375 ID=MS.gene51841
chr7.4 mRNA 28416375 28418638 28416375 ID=MS.gene51841.t1;Parent=MS.gene51841
chr7.4 exon 28416375 28417210 28416375 ID=MS.gene51841.t1.exon1;Parent=MS.gene51841.t1
chr7.4 CDS 28416375 28417210 28416375 ID=cds.MS.gene51841.t1;Parent=MS.gene51841.t1
chr7.4 exon 28417302 28417727 28417302 ID=MS.gene51841.t1.exon2;Parent=MS.gene51841.t1
chr7.4 CDS 28417302 28417727 28417302 ID=cds.MS.gene51841.t1;Parent=MS.gene51841.t1
chr7.4 exon 28418048 28418363 28418048 ID=MS.gene51841.t1.exon3;Parent=MS.gene51841.t1
chr7.4 CDS 28418048 28418363 28418048 ID=cds.MS.gene51841.t1;Parent=MS.gene51841.t1
chr7.4 exon 28418591 28418638 28418591 ID=MS.gene51841.t1.exon4;Parent=MS.gene51841.t1
chr7.4 CDS 28418591 28418638 28418591 ID=cds.MS.gene51841.t1;Parent=MS.gene51841.t1
Gene Sequence

>MS.gene51841

ATGGATAAAGAGGAATCCCCACAAATCCCAAGAAGAACGACAAGGTCTTTATCTACGCCATCCACTTCAAATATTGTACCTGCAGCAAAAGTAAACAGCAGCCTTGAATCCCTAACAATTAATGACCTTTTAGTTGGAGGAGACTCTATTAGCCTAGATGATATAATTTCTAGTTTTCCTGGAAGAAGTAGTCAGATTCACGAGATTGTGCGTCTTTTGGGACCGTTGAATTCCCCATTACTTCCTTTGTTTGTTTATGGAGGTGCTTCTACGGGAAAAACCAGTATCATTCTTCAATTATTCAGACATCTCAATAGGCCTCTTGTTTATTCTAGTTGTAGGACATGTTATAACCAGCGTATCTTGTTCGAGTCTGTTCTGAATCAGTTACTTCTCCATAGAAAAAATGCTGCCAATTGTTATGCTAATGCGAAGCGCTGTGAGAGACCGTCTGATTTTGTCAATTTTCTTCGTGAAACATTAACCGGTGTTATAAATAATCTGAAGGAGAAATCAGAGAAGTTGATCCCGAAAAAGAAGATACATGGTGGGGGAACAGGAAACATGATCTACTTGGTATTTGACAATTTTCATCTTGTTAGAGATTGGGATAAAAGCTCGACTATATTGCCCTTGCTGTTTAATCTCTATGATATGCTAAAGATGCCTGAGGTTGGTCTGATTTTCATCAGCAGTACTTCACCAGATACCTTTTACACTAACATGGGTTATGTGGAGCCTATCCCCATTTACTTTCCTGATTACACAGAAGGTGATATTCGTCAAATCCTATTGAGGAACCAAGCAAACCAGAAGTTGTATTCGTCATTTCTCGAGTAAGACATTTGTTTTTATCACTAATATCAGATTCCATTTTAAGCATTACATGATCTGATATATTGAAGAATTTTGTTTCTTTTCATGCAGTGTAGCTCTGAAGCCTTTCTGTAGAATTACTAGGCAGGTTGGTGAATTGTCTACTGCATTAAAGCCACTATTTGAAAAATATTGTGAACCTCTAAGTGATAAGGGAAAAGGAGTTGTCCCTAGTGAAGACATGAAGAGGAGGCTATTCAATCATATCAAGCCGCATATTGCCTCCTCTTTGAACGATGTATTTAAGGTTTCATCTGCTCCTTCGAGTGAAGTTGAAACTGGTAAAGAGACGAAGCATAAGGGGAATCTGAAGAGATTGGAGAGATCTGAGGAAATCGGTGATCTTGATTTTCACATGTCTACTAGTGCAAAGTATCTTCTTATTTCAGCATTTCTTGCCTCTAGAAACCCTGCTACTCTTGATGCTTCACTTTTTGATTCCAAAGGTGGTTCTGATAATCGAAAGCGAAAGAGGAAGTAAGTATGGCGATTTCAAACCATTTTTTGATGTGCTGAAAACAATATTGTGTATGAATTTTTTAGACTGAAATACTTCTTTATTATTTGAATGAAGCCTTTTGTTGGTTTTATTTTTCTTTTACTTATTTCCATTTTCATTTTTTCCTGTGAGAAGAGTTATTTATTGAAGTTCCAACCATTGTCTGAATGGCTTTGTTTTTTTTTTTTTTTTTTTTTTGTAGTTGATTGATAAGATTTAGGTATCTTCCCATAGTTCATTCTTATTGAGCATTGCTCATTTATTGTTGTTGTCTGAATATGCTTTTTGATGCATACAGGGCCACTGAAAAAGCGCTGGAAAAGAAGGAAATGTTGGAAGAGGAACTACTAATGAAAGGACCTGGAACTTTTCCACTGGAGAGGTTGATAGCCATCTTTCAGTGCATTGTTTCTGTTGCAGAAGACCCAGCTGATGAAGATGAACAAAAGAATGAAGAACTAGGAGCTCAATGTGGTAGCGGTTCGCTGATGTCTGACGTTCTTTTGCAGCTATCCACTCTCTGCAATGCTAATTTTATTTTTAAGGGAAAAAGCTGCCCAATTGAAGGCTCAACTCGGTATCGATCAACTATATCTGAAGACTTGGTCTTAAAGGTAACATCTTTGTCTCGCTCACGTAATACTCTTTTTTTTTTGTTATTGTTTGTGTATTTGAAATTCAATGCTCGCGTACATGTGTAATAAAGGATAATAATTTCAACATTGTTTAAGCAGGTTGCAAGGAGCCTTAAGTTCCCATTGTCAAAGTATTTATACAGAAGTTAGTAACCTTTAACAATGACAGCTTCGCGGAGCTTGGTTCTCAAATTTTGTTCTGATAGATCTACCAAGAGCGGTCTACAACCTTCAATATGATCTTCGGTTATTAA
Protein sequence

>MS.gene51841.t1

MDKEESPQIPRRTTRSLSTPSTSNIVPAAKVNSSLESLTINDLLVGGDSISLDDIISSFPGRSSQIHEIVRLLGPLNSPLLPLFVYGGASTGKTSIILQLFRHLNRPLVYSSCRTCYNQRILFESVLNQLLLHRKNAANCYANAKRCERPSDFVNFLRETLTGVINNLKEKSEKLIPKKKIHGGGTGNMIYLVFDNFHLVRDWDKSSTILPLLFNLYDMLKMPEVGLIFISSTSPDTFYTNMGYVEPIPIYFPDYTEGDIRQILLRNQANQKLYSSFLDVALKPFCRITRQVGELSTALKPLFEKYCEPLSDKGKGVVPSEDMKRRLFNHIKPHIASSLNDVFKVSSAPSSEVETGKETKHKGNLKRLERSEEIGDLDFHMSTSAKYLLISAFLASRNPATLDASLFDSKGGSDNRKRKRKATEKALEKKEMLEEELLMKGPGTFPLERLIAIFQCIVSVAEDPADEDEQKNEELGAQCGSGSLMSDVLLQLSTLCNANFIFKGKSCPIEGSTRYRSTISEDLVLKIYQERSTTFNMIFGY