Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52755.t1 | XP_013449711.1 | 88.7 | 71 | 4 | 1 | 1 | 67 | 1 | 71 | 1.00E-21 | 112.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52755.t1 | Q9SVC4 | 65.5 | 55 | 16 | 1 | 1 | 52 | 1 | 55 | 1.5e-10 | 66.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52755.t1 | I3SV27 | 88.7 | 71 | 4 | 1 | 1 | 67 | 1 | 71 | 7.4e-22 | 112.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054917 | MS.gene52755 | 0.802681 | 5.00E-49 | -1.69E-46 |
| MS.gene055116 | MS.gene52755 | 0.801252 | 9.83E-49 | -1.69E-46 |
| MS.gene057124 | MS.gene52755 | 0.80278 | 4.77E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52755.t1 | MTR_7g094710 | 100.000 | 47 | 0 | 0 | 1 | 47 | 1 | 47 | 3.65e-28 | 96.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52755.t1 | AT3G52420 | 65.455 | 55 | 16 | 1 | 1 | 52 | 1 | 55 | 1.35e-17 | 69.3 |
Find 25 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAAGCGGTTGTGGTTGC+TGG | 0.323695 | 7.2:-14950065 | MS.gene52755:CDS |
| CTGGAGCCTTAGCATTTGTC+TGG | 0.357220 | 7.2:-14950046 | MS.gene52755:CDS |
| GCATCTCCGGTATCAGCTTC+AGG | 0.383647 | 7.2:+14949918 | None:intergenic |
| GCTTCAGGAGCGGCGGCAGC+AGG | 0.386627 | 7.2:+14949933 | None:intergenic |
| TGCAGTTGCGGTGGCATCTC+CGG | 0.432441 | 7.2:+14949905 | None:intergenic |
| GCGGCAGCAGGAACATCATC+AGG | 0.447838 | 7.2:+14949945 | None:intergenic |
| TAGAGTCGCGGGTCTGAGAA+AGG | 0.465845 | 7.2:+14949994 | None:intergenic |
| CATCATCAGGATCCCGGGTC+GGG | 0.469255 | 7.2:+14949958 | None:intergenic |
| TCGACAAATCTGACCCGACC+CGG | 0.479388 | 7.2:-14949971 | MS.gene52755:CDS |
| CAGGAACATCATCAGGATCC+CGG | 0.516320 | 7.2:+14949952 | None:intergenic |
| GCCGCTCCTGAAGCTGATAC+CGG | 0.517196 | 7.2:-14949924 | MS.gene52755:CDS |
| AAATTAGGCGTCTGCAGTTG+CGG | 0.528752 | 7.2:+14949893 | None:intergenic |
| GTCGCGGGTCTGAGAAAGGA+AGG | 0.546452 | 7.2:+14949998 | None:intergenic |
| AGGAACATCATCAGGATCCC+GGG | 0.566071 | 7.2:+14949953 | None:intergenic |
| AAAGGCAAAAGAAGCGGTTG+TGG | 0.575609 | 7.2:-14950072 | MS.gene52755:CDS |
| CGACAAATCTGACCCGACCC+GGG | 0.576446 | 7.2:-14949970 | MS.gene52755:CDS |
| AGCGAGCCAGACAAATGCTA+AGG | 0.593697 | 7.2:+14950040 | None:intergenic |
| AGATTTGTCGATAGAGTCGC+GGG | 0.604367 | 7.2:+14949983 | None:intergenic |
| ACATCATCAGGATCCCGGGT+CGG | 0.617499 | 7.2:+14949957 | None:intergenic |
| CAGATTTGTCGATAGAGTCG+CGG | 0.623137 | 7.2:+14949982 | None:intergenic |
| TTAGGCGTCTGCAGTTGCGG+TGG | 0.639439 | 7.2:+14949896 | None:intergenic |
| TCCGGTATCAGCTTCAGGAG+CGG | 0.639504 | 7.2:+14949923 | None:intergenic |
| GGTATCAGCTTCAGGAGCGG+CGG | 0.662347 | 7.2:+14949926 | None:intergenic |
| GATGGGAAAGGCAAAAGAAG+CGG | 0.676214 | 7.2:-14950078 | MS.gene52755:CDS |
| TCGCGGGTCTGAGAAAGGAA+GGG | 0.692341 | 7.2:+14949999 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGGCAAAAGAAGCGGTTG+TGG | - | chr7.2:14949901-14949920 | MS.gene52755:CDS | 45.0% | |
| AGATTTGTCGATAGAGTCGC+GGG | + | chr7.2:14949993-14950012 | None:intergenic | 45.0% | |
| CAGATTTGTCGATAGAGTCG+CGG | + | chr7.2:14949994-14950013 | None:intergenic | 45.0% | |
| AAAGAAGCGGTTGTGGTTGC+TGG | - | chr7.2:14949908-14949927 | MS.gene52755:CDS | 50.0% | |
| AGCGAGCCAGACAAATGCTA+AGG | + | chr7.2:14949936-14949955 | None:intergenic | 50.0% | |
| AGGAACATCATCAGGATCCC+GGG | + | chr7.2:14950023-14950042 | None:intergenic | 50.0% | |
| CAGGAACATCATCAGGATCC+CGG | + | chr7.2:14950024-14950043 | None:intergenic | 50.0% | |
| CTGGAGCCTTAGCATTTGTC+TGG | - | chr7.2:14949927-14949946 | MS.gene52755:CDS | 50.0% | |
| ACATCATCAGGATCCCGGGT+CGG | + | chr7.2:14950019-14950038 | None:intergenic | 55.0% | |
| GCATCTCCGGTATCAGCTTC+AGG | + | chr7.2:14950058-14950077 | None:intergenic | 55.0% | |
| TAGAGTCGCGGGTCTGAGAA+AGG | + | chr7.2:14949982-14950001 | None:intergenic | 55.0% | |
| TCCGGTATCAGCTTCAGGAG+CGG | + | chr7.2:14950053-14950072 | None:intergenic | 55.0% | |
| TCGACAAATCTGACCCGACC+CGG | - | chr7.2:14950002-14950021 | MS.gene52755:CDS | 55.0% | |
| TCGCGGGTCTGAGAAAGGAA+GGG | + | chr7.2:14949977-14949996 | None:intergenic | 55.0% | |
| CATCATCAGGATCCCGGGTC+GGG | + | chr7.2:14950018-14950037 | None:intergenic | 60.0% | |
| CGACAAATCTGACCCGACCC+GGG | - | chr7.2:14950003-14950022 | MS.gene52755:CDS | 60.0% | |
| GCCGCTCCTGAAGCTGATAC+CGG | - | chr7.2:14950049-14950068 | MS.gene52755:CDS | 60.0% | |
| GCGGCAGCAGGAACATCATC+AGG | + | chr7.2:14950031-14950050 | None:intergenic | 60.0% | |
| GGTATCAGCTTCAGGAGCGG+CGG | + | chr7.2:14950050-14950069 | None:intergenic | 60.0% | |
| GTCGCGGGTCTGAGAAAGGA+AGG | + | chr7.2:14949978-14949997 | None:intergenic | 60.0% | |
| TGCAGTTGCGGTGGCATCTC+CGG | + | chr7.2:14950071-14950090 | None:intergenic | 60.0% | |
| TTAGGCGTCTGCAGTTGCGG+TGG | + | chr7.2:14950080-14950099 | None:intergenic | 60.0% | |
| GCTTCAGGAGCGGCGGCAGC+AGG | + | chr7.2:14950043-14950062 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 14949896 | 14950099 | 14949896 | ID=MS.gene52755 |
| chr7.2 | mRNA | 14949896 | 14950099 | 14949896 | ID=MS.gene52755.t1;Parent=MS.gene52755 |
| chr7.2 | exon | 14949896 | 14950099 | 14949896 | ID=MS.gene52755.t1.exon1;Parent=MS.gene52755.t1 |
| chr7.2 | CDS | 14949896 | 14950099 | 14949896 | ID=cds.MS.gene52755.t1;Parent=MS.gene52755.t1 |
| Gene Sequence |
| Protein sequence |
