Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52824.t1 | XP_003590905.1 | 99.7 | 906 | 3 | 0 | 1 | 906 | 1 | 906 | 1.20E-279 | 972.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52824.t1 | Q9M8Y0 | 88.3 | 891 | 99 | 2 | 21 | 906 | 7 | 897 | 1.2e-219 | 764.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52824.t1 | G7I5N5 | 99.7 | 906 | 3 | 0 | 1 | 906 | 1 | 906 | 8.9e-280 | 972.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene23932 | MS.gene52824 | PPI |
| MS.gene014576 | MS.gene52824 | PPI |
| MS.gene93994 | MS.gene52824 | PPI |
| MS.gene93993 | MS.gene52824 | PPI |
| MS.gene40223 | MS.gene52824 | PPI |
| MS.gene071051 | MS.gene52824 | PPI |
| MS.gene52824 | MS.gene93890 | PPI |
| MS.gene97109 | MS.gene52824 | PPI |
| MS.gene69092 | MS.gene52824 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52824.t1 | MTR_1g079510 | 99.669 | 906 | 3 | 0 | 1 | 906 | 1 | 906 | 0.0 | 1890 |
| MS.gene52824.t1 | MTR_1g059610 | 27.614 | 746 | 462 | 13 | 178 | 901 | 96 | 785 | 1.63e-72 | 257 |
| MS.gene52824.t1 | MTR_1g059610 | 30.904 | 343 | 189 | 3 | 148 | 442 | 98 | 440 | 5.32e-40 | 160 |
| MS.gene52824.t1 | MTR_1g045490 | 23.411 | 299 | 196 | 2 | 140 | 431 | 201 | 473 | 4.55e-12 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52824.t1 | AT3G04240 | 82.828 | 891 | 148 | 2 | 21 | 906 | 7 | 897 | 0.0 | 1579 |
| MS.gene52824.t1 | AT3G04240 | 86.446 | 664 | 90 | 0 | 243 | 906 | 1 | 664 | 0.0 | 1247 |
| MS.gene52824.t1 | AT3G04240 | 32.362 | 309 | 200 | 2 | 175 | 474 | 1 | 309 | 2.12e-41 | 163 |
| MS.gene52824.t1 | AT3G04240 | 33.103 | 290 | 194 | 0 | 74 | 363 | 2 | 291 | 8.16e-41 | 161 |
| MS.gene52824.t1 | AT3G11540 | 27.979 | 747 | 462 | 14 | 177 | 902 | 92 | 783 | 4.34e-71 | 253 |
| MS.gene52824.t1 | AT3G11540 | 31.549 | 355 | 183 | 5 | 141 | 442 | 90 | 437 | 2.94e-39 | 157 |
| MS.gene52824.t1 | AT3G11540 | 32.830 | 265 | 171 | 1 | 116 | 373 | 172 | 436 | 1.82e-34 | 142 |
| MS.gene52824.t1 | AT3G11540 | 26.480 | 321 | 213 | 5 | 239 | 545 | 50 | 361 | 1.65e-19 | 94.7 |
| MS.gene52824.t1 | AT3G11540 | 28.346 | 254 | 168 | 4 | 78 | 317 | 196 | 449 | 7.99e-19 | 92.4 |
| MS.gene52824.t1 | AT3G11540 | 28.700 | 223 | 149 | 3 | 295 | 510 | 41 | 260 | 6.28e-16 | 83.2 |
| MS.gene52824.t1 | AT3G11540 | 26.045 | 622 | 385 | 15 | 295 | 902 | 41 | 601 | 1.11e-42 | 167 |
| MS.gene52824.t1 | AT3G11540 | 31.694 | 183 | 113 | 2 | 198 | 373 | 77 | 254 | 1.82e-16 | 84.7 |
| MS.gene52824.t1 | AT3G11540 | 31.176 | 170 | 103 | 3 | 177 | 339 | 92 | 254 | 1.86e-13 | 75.1 |
| MS.gene52824.t1 | AT3G11540 | 26.540 | 211 | 143 | 2 | 239 | 442 | 50 | 255 | 1.19e-11 | 69.3 |
| MS.gene52824.t1 | AT3G11540 | 31.250 | 192 | 103 | 6 | 141 | 317 | 90 | 267 | 4.48e-11 | 67.4 |
| MS.gene52824.t1 | AT2G32450 | 28.713 | 202 | 136 | 2 | 180 | 373 | 245 | 446 | 1.35e-14 | 79.0 |
| MS.gene52824.t1 | AT2G32450 | 27.976 | 168 | 121 | 0 | 196 | 363 | 303 | 470 | 8.41e-13 | 72.8 |
| MS.gene52824.t1 | AT2G32450 | 24.481 | 241 | 174 | 1 | 199 | 431 | 230 | 470 | 1.27e-12 | 72.4 |
| MS.gene52824.t1 | AT1G05150 | 24.206 | 252 | 165 | 1 | 180 | 431 | 250 | 475 | 3.71e-13 | 73.9 |
| MS.gene52824.t1 | AT1G05150 | 25.094 | 267 | 172 | 2 | 248 | 512 | 250 | 490 | 1.54e-12 | 72.0 |
| MS.gene52824.t1 | AT5G63200 | 21.594 | 389 | 231 | 10 | 117 | 434 | 218 | 603 | 1.78e-11 | 68.6 |
Find 227 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAATCCTTATCAACCTTAA+TGG | 0.143162 | 1.1:-59350789 | MS.gene52824:CDS |
| GGCTGAAGCTCATGCTAATT+TGG | 0.170309 | 1.1:-59353617 | MS.gene52824:CDS |
| AAATGATGTTACAGGTAATT+TGG | 0.170385 | 1.1:-59354794 | MS.gene52824:intron |
| TTTGGAAGCTTACAATAATT+TGG | 0.173624 | 1.1:-59354692 | MS.gene52824:intron |
| TGACCATGCATCAGCAAAAT+TGG | 0.177023 | 1.1:+59357335 | None:intergenic |
| TACCATGTATTAAATATTTC+AGG | 0.201953 | 1.1:+59349092 | None:intergenic |
| CCACAGGAACAGATATATTA+TGG | 0.228996 | 1.1:-59348435 | MS.gene52824:CDS |
| CAATGCTTGCTCTGGAAATT+AGG | 0.230192 | 1.1:-59351428 | MS.gene52824:intron |
| ACCAGTTCAAGTTTCATATA+TGG | 0.238851 | 1.1:-59349563 | MS.gene52824:CDS |
| TACTCTGCCTCTTGAGAAAA+TGG | 0.256247 | 1.1:-59348395 | MS.gene52824:CDS |
| CTACCTTTGTATAACCATTA+AGG | 0.273432 | 1.1:+59350775 | None:intergenic |
| TAATTTGGCTTCTGCCTATA+AGG | 0.274347 | 1.1:-59353602 | MS.gene52824:CDS |
| ATTAGCATACCTATCCTTAT+AGG | 0.292089 | 1.1:+59353588 | None:intergenic |
| CCAAACTATGGCATGGCTTA+TGG | 0.303947 | 1.1:-59355588 | MS.gene52824:intron |
| TGCCGAGTGTTACGGTAATA+TGG | 0.304813 | 1.1:-59358055 | MS.gene52824:CDS |
| CATGTATATATTTCCAAGAT+TGG | 0.315239 | 1.1:+59354169 | None:intergenic |
| TAACCTCCCTCCTGCTTGAT+TGG | 0.315790 | 1.1:+59351231 | None:intergenic |
| GTTTCATATATGGGATTTCC+TGG | 0.320555 | 1.1:-59349553 | MS.gene52824:CDS |
| CCAGTTCAAGTTTCATATAT+GGG | 0.320872 | 1.1:-59349562 | MS.gene52824:CDS |
| CTCTGTATTTCCACTGGACT+GGG | 0.331322 | 1.1:-59348352 | MS.gene52824:CDS |
| AATAGTAAAATTACCATTAG+AGG | 0.332566 | 1.1:+59357234 | None:intergenic |
| AGAAGATTGCAAGTTGCTTC+AGG | 0.349834 | 1.1:+59353191 | None:intergenic |
| CTCCATTCAGTACCATCATT+TGG | 0.353434 | 1.1:+59350903 | None:intergenic |
| GATCGTTTGGGCTGACAGTT+TGG | 0.362406 | 1.1:+59349179 | None:intergenic |
| AAACAGAGGCAATACTTATA+AGG | 0.364549 | 1.1:-59353704 | MS.gene52824:CDS |
| CTTCAGACACGGCCAAACTA+TGG | 0.368387 | 1.1:-59355600 | MS.gene52824:CDS |
| CCTGATGCATACTTGAACCT+TGG | 0.368615 | 1.1:-59355780 | MS.gene52824:CDS |
| CTCTTGAAATTTCCTGCAGC+AGG | 0.374684 | 1.1:-59348921 | MS.gene52824:CDS |
| CAATCTTCTACATACACTTC+AGG | 0.378736 | 1.1:-59353177 | MS.gene52824:intron |
| CCACAAGCGTTGACCAATCT+TGG | 0.379245 | 1.1:-59354182 | MS.gene52824:CDS |
| CTTTGTAAACGATTATAAAC+AGG | 0.383696 | 1.1:-59349327 | MS.gene52824:intron |
| ATTATAGCAAGGTTGTTATA+CGG | 0.385320 | 1.1:+59353989 | None:intergenic |
| TATAACCATTAAGGTTGATA+AGG | 0.388324 | 1.1:+59350784 | None:intergenic |
| ATAGGAGCAGGATGGCTAAA+TGG | 0.389099 | 1.1:+59351255 | None:intergenic |
| ACTTGCTATCAACCCTCTAA+TGG | 0.389345 | 1.1:-59357247 | MS.gene52824:intron |
| AAATCTTGCTGGTCTGTTCA+TGG | 0.391501 | 1.1:-59356209 | MS.gene52824:CDS |
| CAGCCCAAACGATCAGATTA+TGG | 0.392917 | 1.1:-59349170 | MS.gene52824:CDS |
| ACTCTGTATTTCCACTGGAC+TGG | 0.395288 | 1.1:-59348353 | MS.gene52824:CDS |
| CCATAAGCCATGCCATAGTT+TGG | 0.399981 | 1.1:+59355588 | None:intergenic |
| CCAAGGTTCAAGTATGCATC+AGG | 0.403218 | 1.1:+59355780 | None:intergenic |
| CAAATGTTCAAGGAAGTAGA+AGG | 0.411950 | 1.1:-59351640 | MS.gene52824:CDS |
| ACATTATGAGCAAGGTCAAC+TGG | 0.412081 | 1.1:-59354764 | MS.gene52824:CDS |
| GCTGGTCTGTTCATGGAGTC+TGG | 0.413134 | 1.1:-59356202 | MS.gene52824:CDS |
| CTTCACAGATGTTGCAATGA+AGG | 0.419095 | 1.1:-59348630 | MS.gene52824:CDS |
| CTATTACTTGATTGCCATTG+AGG | 0.423798 | 1.1:-59357875 | MS.gene52824:intron |
| GAGACCATAATCTGATCGTT+TGG | 0.428281 | 1.1:+59349166 | None:intergenic |
| CTCTTGAGAAAATGGCTACT+AGG | 0.429756 | 1.1:-59348387 | MS.gene52824:CDS |
| ATTGCATGGTCAAATCTTGC+TGG | 0.431271 | 1.1:-59356220 | MS.gene52824:CDS |
| CAAGATTGGTCAACGCTTGT+GGG | 0.432953 | 1.1:+59354183 | None:intergenic |
| TTGGCTCATCCGACAGCTCT+CGG | 0.434068 | 1.1:-59358750 | MS.gene52824:CDS |
| ATGCTATGGCATGGAAAGGC+TGG | 0.434506 | 1.1:+59351463 | None:intergenic |
| ATCTGAAACAGAGCACTTTG+TGG | 0.435863 | 1.1:-59350868 | MS.gene52824:CDS |
| CCTTGGTGCAATTTATTACC+AGG | 0.442505 | 1.1:-59358246 | MS.gene52824:intron |
| GACCATGCATCAGCAAAATT+GGG | 0.445415 | 1.1:+59357336 | None:intergenic |
| CCAAGATTGGTCAACGCTTG+TGG | 0.445603 | 1.1:+59354182 | None:intergenic |
| AAAATGGCTACTAGGGTTGC+TGG | 0.449044 | 1.1:-59348379 | MS.gene52824:CDS |
| CTGATGCATACTTGAACCTT+GGG | 0.451840 | 1.1:-59355779 | MS.gene52824:CDS |
| AACCACCACTCTCAGCTTGT+TGG | 0.452650 | 1.1:-59358769 | MS.gene52824:CDS |
| GAACGGTTCAACGCGATCTC+CGG | 0.452834 | 1.1:+59358717 | None:intergenic |
| ACCAAGGAGTAGTAAATTAT+CGG | 0.453046 | 1.1:+59358262 | None:intergenic |
| ACCTTGCTCATAATGTATAC+TGG | 0.453921 | 1.1:+59354771 | None:intergenic |
| CACAGGAACAGATATATTAT+GGG | 0.455571 | 1.1:-59348434 | MS.gene52824:CDS |
| CCCATATATGAAACTTGAAC+TGG | 0.456705 | 1.1:+59349562 | None:intergenic |
| CTTTCAGATGCTGCTGCACA+AGG | 0.457171 | 1.1:-59348674 | MS.gene52824:intron |
| GACCTCATTGTAGCAAGATA+TGG | 0.458721 | 1.1:+59353762 | None:intergenic |
| AGCAGTGTGCAGCATATTTG+CGG | 0.459706 | 1.1:+59351307 | None:intergenic |
| GGCAATACTTATAAGGAGAT+TGG | 0.459746 | 1.1:-59353697 | MS.gene52824:CDS |
| ATGGGATTTCCTGGCACAAC+CGG | 0.462984 | 1.1:-59349544 | MS.gene52824:CDS |
| CCCTCCTGCTTGATTGGAAT+AGG | 0.463550 | 1.1:+59351237 | None:intergenic |
| CGCGCTCATGATTCTAGTGA+AGG | 0.464261 | 1.1:-59358652 | MS.gene52824:intron |
| AGACCATAATCTGATCGTTT+GGG | 0.464870 | 1.1:+59349167 | None:intergenic |
| TAATGCAGTGTGTCTGTTGT+TGG | 0.467039 | 1.1:-59351675 | MS.gene52824:intron |
| CGGAATCATGATCTCCGTCC+AGG | 0.468061 | 1.1:-59358815 | None:intergenic |
| GGCTCACTCTGTATTTCCAC+TGG | 0.472212 | 1.1:-59348358 | MS.gene52824:CDS |
| CAAGAGCCAAAGCGCGCTGT+TGG | 0.474016 | 1.1:+59348938 | None:intergenic |
| ACTTGACCCAATGCTTGCTC+TGG | 0.474813 | 1.1:-59351436 | MS.gene52824:CDS |
| CCACACTTTGCCGAGTGTTA+CGG | 0.474939 | 1.1:-59358063 | MS.gene52824:CDS |
| AGCTCTCGGTTGCCGTTCAC+CGG | 0.478100 | 1.1:-59358736 | MS.gene52824:CDS |
| TAGAGTCAGTGATGCTATCC+AGG | 0.479729 | 1.1:-59353674 | MS.gene52824:CDS |
| CCACCCATTGTCACATCTCA+TGG | 0.482380 | 1.1:-59351070 | MS.gene52824:CDS |
| AAGAGCCAAAGCGCGCTGTT+GGG | 0.483592 | 1.1:+59348939 | None:intergenic |
| TATGAAACTTGAACTGGTGC+AGG | 0.484055 | 1.1:+59349568 | None:intergenic |
| ACCGATAATTTACTACTCCT+TGG | 0.486401 | 1.1:-59358263 | MS.gene52824:CDS |
| GTAATCTATGTATGTAGCTC+CGG | 0.486466 | 1.1:+59349525 | None:intergenic |
| GGTTATGAAAGACTAAGGAT+TGG | 0.487687 | 1.1:-59351213 | MS.gene52824:CDS |
| CGACGACAACTCTTGCTTAA+CGG | 0.493227 | 1.1:+59358693 | None:intergenic |
| GGTTGATGCTCATAGTAACT+TGG | 0.496415 | 1.1:-59357060 | MS.gene52824:CDS |
| AGCTACATACATAGATTACT+TGG | 0.496829 | 1.1:-59349521 | MS.gene52824:CDS |
| CTTCAATCAACTTTACAAGA+TGG | 0.496856 | 1.1:-59349117 | MS.gene52824:CDS |
| GCATGAGCTTCAGCCATAGT+AGG | 0.497608 | 1.1:+59353625 | None:intergenic |
| CAAAGTAGCAATGCGGGAGA+TGG | 0.497765 | 1.1:+59349345 | None:intergenic |
| TTCAGATGCTGCTGCACAAG+GGG | 0.497876 | 1.1:-59348672 | MS.gene52824:intron |
| TAATTTCCAGAGCAAGCATT+GGG | 0.501643 | 1.1:+59351430 | None:intergenic |
| TATGTAGCTCCGGTTGTGCC+AGG | 0.502858 | 1.1:+59349535 | None:intergenic |
| GTTATGAAAGACTAAGGATT+GGG | 0.503729 | 1.1:-59351212 | MS.gene52824:intron |
| TCTGTGAAGATAATTTGATC+AGG | 0.505192 | 1.1:+59348644 | None:intergenic |
| AGTCTCATTTCTCCTGCTGC+AGG | 0.505262 | 1.1:+59348909 | None:intergenic |
| TGCTTGATTGGAATAGGAGC+AGG | 0.506321 | 1.1:+59351243 | None:intergenic |
| GGTCCTCCGCATTGATCCCT+TGG | 0.509269 | 1.1:-59353743 | MS.gene52824:CDS |
| ATTGATCCCTTGGCAGCTGA+TGG | 0.509463 | 1.1:-59353733 | MS.gene52824:CDS |
| AATGCAGTGTGTCTGTTGTT+GGG | 0.510745 | 1.1:-59351674 | MS.gene52824:intron |
| GGCAGACCTTGTAATACTGC+AGG | 0.511471 | 1.1:+59356164 | None:intergenic |
| GGTAATCTAATGAAAGCTCA+AGG | 0.514812 | 1.1:-59357038 | MS.gene52824:CDS |
| TGCTATAATATATAAGCAGC+AGG | 0.515077 | 1.1:-59353975 | MS.gene52824:intron |
| GCATGGAAAGGCTGGACACT+AGG | 0.517313 | 1.1:+59351471 | None:intergenic |
| CGTTTACAAAGTAGCAATGC+GGG | 0.517564 | 1.1:+59349339 | None:intergenic |
| AACTCTTGCTTAACGGCGAA+CGG | 0.519318 | 1.1:+59358700 | None:intergenic |
| TAATCGCACGTATGTAATCC+TGG | 0.519833 | 1.1:+59353656 | None:intergenic |
| CAATCTTGGAAATATATACA+TGG | 0.519958 | 1.1:-59354168 | MS.gene52824:CDS |
| TGCTATCGTATCTGATGTCA+TGG | 0.520898 | 1.1:+59350836 | None:intergenic |
| TACAAGACCATCAGCTGCCA+AGG | 0.521518 | 1.1:+59353726 | None:intergenic |
| GATTACCGTCAGGCCTACTA+TGG | 0.522671 | 1.1:-59353638 | MS.gene52824:CDS |
| GTGTACCCAACAGCGCGCTT+TGG | 0.522743 | 1.1:-59348944 | MS.gene52824:CDS |
| ATCAAGAATACATACCTCAA+TGG | 0.523924 | 1.1:+59357861 | None:intergenic |
| CTAATTTCCAGAGCAAGCAT+TGG | 0.530198 | 1.1:+59351429 | None:intergenic |
| AACCTACCTTTGCTATTGCA+TGG | 0.530684 | 1.1:-59356234 | MS.gene52824:CDS |
| TTCACAGATGTTGCAATGAA+GGG | 0.531625 | 1.1:-59348629 | MS.gene52824:CDS |
| CAGAGCCCTGCAGTATTACA+AGG | 0.533229 | 1.1:-59356170 | MS.gene52824:intron |
| CTGTATTTCCACTGGACTGG+GGG | 0.534881 | 1.1:-59348350 | MS.gene52824:CDS |
| AGTACCATCATTTGGACTCA+AGG | 0.534981 | 1.1:+59350911 | None:intergenic |
| TGGCTTCTGCCTATAAGGAT+AGG | 0.535459 | 1.1:-59353597 | MS.gene52824:intron |
| TTGGAAATATATACATGGAA+TGG | 0.538270 | 1.1:-59354163 | MS.gene52824:intron |
| AGGGAGGTTATGAAAGACTA+AGG | 0.540074 | 1.1:-59351218 | MS.gene52824:CDS |
| AAGAACATACATTATAGCAC+TGG | 0.540515 | 1.1:+59354320 | None:intergenic |
| TCTTGAGAAAATGGCTACTA+GGG | 0.544333 | 1.1:-59348386 | MS.gene52824:CDS |
| GTCAAGAAATAAGTCTGCTA+AGG | 0.547289 | 1.1:+59348586 | None:intergenic |
| CTAGAATCATGAGCGCGTAA+AGG | 0.548037 | 1.1:+59358658 | None:intergenic |
| GTGGTATGTGCATTGCATAA+AGG | 0.548073 | 1.1:+59348454 | None:intergenic |
| GCCAGTATACATTATGAGCA+AGG | 0.548161 | 1.1:-59354772 | MS.gene52824:CDS |
| TGCTCCTATTCCAATCAAGC+AGG | 0.555496 | 1.1:-59351241 | MS.gene52824:CDS |
| TTTCAGATGCTGCTGCACAA+GGG | 0.555667 | 1.1:-59348673 | MS.gene52824:intron |
| ATAGTAAAATTACCATTAGA+GGG | 0.557033 | 1.1:+59357235 | None:intergenic |
| GGTAATGCTCTGAAAGATGT+TGG | 0.562829 | 1.1:-59354361 | MS.gene52824:CDS |
| ATTATGGGCAGGTCTGCCTA+TGG | 0.564345 | 1.1:-59348419 | MS.gene52824:CDS |
| GGAACAGATATATTATGGGC+AGG | 0.566521 | 1.1:-59348430 | MS.gene52824:CDS |
| TGGGTCAAGTGGATATGCTA+TGG | 0.567625 | 1.1:+59351449 | None:intergenic |
| TGCCATATTACCGTAACACT+CGG | 0.568106 | 1.1:+59358053 | None:intergenic |
| TGAGCAAGGTCAACTGGACA+TGG | 0.570022 | 1.1:-59354758 | MS.gene52824:CDS |
| AGAGCAAGCATTGGGTCAAG+TGG | 0.574030 | 1.1:+59351438 | None:intergenic |
| GGCTGTAAAGAGCTACAGAC+AGG | 0.576286 | 1.1:-59353237 | MS.gene52824:CDS |
| GCTACACTAAATGTAACCAC+TGG | 0.577805 | 1.1:-59354022 | MS.gene52824:CDS |
| TTATTTCAGTGGACATGTAG+AGG | 0.579165 | 1.1:-59353261 | MS.gene52824:intron |
| GGTGGTTGTAGTGGTGGCGA+TGG | 0.580159 | 1.1:+59358785 | None:intergenic |
| ATCCTGAAATATTTAATACA+TGG | 0.581813 | 1.1:-59349094 | MS.gene52824:intron |
| GGATCGTGATCAAATGTTCA+AGG | 0.582624 | 1.1:-59351650 | MS.gene52824:CDS |
| GTTGATGCTCATAGTAACTT+GGG | 0.583189 | 1.1:-59357059 | MS.gene52824:CDS |
| AAGTATGCATCAGGGAAGGA+AGG | 0.583668 | 1.1:+59355789 | None:intergenic |
| GAACCTTGGGAATGTATACA+AGG | 0.587196 | 1.1:-59355766 | MS.gene52824:intron |
| TACGCATGTATGCACTAGCT+AGG | 0.587484 | 1.1:+59357310 | None:intergenic |
| GCTAGTGCATACATGCGTAA+AGG | 0.588429 | 1.1:-59357306 | MS.gene52824:CDS |
| GGTGGCGATGGTGATCTCCC+TGG | 0.590906 | 1.1:+59358797 | None:intergenic |
| ATCCCATGAGATGTGACAAT+GGG | 0.592439 | 1.1:+59351067 | None:intergenic |
| AGATTACTTGGTCACTGATG+AGG | 0.592637 | 1.1:-59349509 | MS.gene52824:intron |
| TAGATATGTGCATACTGAAG+AGG | 0.593648 | 1.1:+59349383 | None:intergenic |
| TCAAGAGGCAGAGTAACCAT+AGG | 0.593677 | 1.1:+59348403 | None:intergenic |
| GTCAACGCTTGTGGGTGGTT+AGG | 0.593743 | 1.1:+59354191 | None:intergenic |
| CAAGTGGATATGCTATGGCA+TGG | 0.594989 | 1.1:+59351454 | None:intergenic |
| CAACCTTAATGGTTATACAA+AGG | 0.596920 | 1.1:-59350778 | MS.gene52824:intron |
| ATCAACATGCTCTTCAGACA+CGG | 0.597820 | 1.1:-59355611 | MS.gene52824:CDS |
| AGCAGGAGAAATGAGACTGA+GGG | 0.597842 | 1.1:-59348904 | MS.gene52824:CDS |
| GGTGGTTAGGCTGTAACGAA+AGG | 0.598171 | 1.1:+59354204 | None:intergenic |
| TGAAGGGTGAACACATTAGA+CGG | 0.599057 | 1.1:-59348613 | MS.gene52824:CDS |
| GCTGAGAGTGGTGGTTGTAG+TGG | 0.599298 | 1.1:+59358776 | None:intergenic |
| TAATATGGCAAATGCATGGA+AGG | 0.599920 | 1.1:-59358040 | MS.gene52824:intron |
| ACATACGTGCGATTACCGTC+AGG | 0.600155 | 1.1:-59353648 | MS.gene52824:CDS |
| TCAACGCGATCTCCGGTGAA+CGG | 0.601206 | 1.1:+59358724 | None:intergenic |
| GAACGGCAACCGAGAGCTGT+CGG | 0.601559 | 1.1:+59358741 | None:intergenic |
| TTACCTTGTATACATTCCCA+AGG | 0.606164 | 1.1:+59355763 | None:intergenic |
| CCATAATATATCTGTTCCTG+TGG | 0.606701 | 1.1:+59348435 | None:intergenic |
| CACCCATTGTCACATCTCAT+GGG | 0.607132 | 1.1:-59351069 | MS.gene52824:CDS |
| TCTGTATTTCCACTGGACTG+GGG | 0.607244 | 1.1:-59348351 | MS.gene52824:CDS |
| GCTGCTTATATATTATAGCA+AGG | 0.607657 | 1.1:+59353978 | None:intergenic |
| TCCTATTCCAATCAAGCAGG+AGG | 0.607994 | 1.1:-59351238 | MS.gene52824:CDS |
| TCTAATGAAAGCTCAAGGGT+TGG | 0.609689 | 1.1:-59357033 | MS.gene52824:CDS |
| GCGATGGTGATCTCCCTGGA+CGG | 0.610099 | 1.1:+59358801 | None:intergenic |
| ACGGTAATATGGCAAATGCA+TGG | 0.610222 | 1.1:-59358044 | MS.gene52824:CDS |
| GATTGGTCAACGCTTGTGGG+TGG | 0.615687 | 1.1:+59354186 | None:intergenic |
| GTCCAAATGATGGTACTGAA+TGG | 0.616666 | 1.1:-59350905 | MS.gene52824:CDS |
| CCGTAACACTCGGCAAAGTG+TGG | 0.618577 | 1.1:+59358063 | None:intergenic |
| ATTTGACCATGCAATAGCAA+AGG | 0.621397 | 1.1:+59356228 | None:intergenic |
| TTATGCAATGCACATACCAC+AGG | 0.622011 | 1.1:-59348451 | MS.gene52824:CDS |
| GGAATCATGATCTCCGTCCA+GGG | 0.622815 | 1.1:-59358814 | None:intergenic |
| GTAATCTAATGAAAGCTCAA+GGG | 0.626437 | 1.1:-59357037 | MS.gene52824:CDS |
| TAACATGCAATAGCTTCTTG+AGG | 0.628478 | 1.1:+59355633 | None:intergenic |
| AAATGTTCAAGGAAGTAGAA+GGG | 0.629997 | 1.1:-59351639 | MS.gene52824:CDS |
| GAAAGATGTTGGCAGAGTGG+AGG | 0.630480 | 1.1:-59354350 | MS.gene52824:CDS |
| GACCATGCAATAGCAAAGGT+AGG | 0.630488 | 1.1:+59356232 | None:intergenic |
| TTCAGCCATAGTAGGCCTGA+CGG | 0.631547 | 1.1:+59353633 | None:intergenic |
| GCAGACCTTGTAATACTGCA+GGG | 0.633448 | 1.1:+59356165 | None:intergenic |
| TGCCATATCTTGCTACAATG+AGG | 0.633633 | 1.1:-59353764 | MS.gene52824:CDS |
| GCACATCAAATGTACAAGAG+TGG | 0.634726 | 1.1:-59358344 | MS.gene52824:CDS |
| GAGAGTGGTGGTTGTAGTGG+TGG | 0.636309 | 1.1:+59358779 | None:intergenic |
| GACACGGCCAAACTATGGCA+TGG | 0.637088 | 1.1:-59355595 | MS.gene52824:CDS |
| TATGCACAACAGAAAGAATG+TGG | 0.638579 | 1.1:-59351034 | MS.gene52824:CDS |
| ATGAGCCAACAAGCTGAGAG+TGG | 0.640591 | 1.1:+59358764 | None:intergenic |
| AGTAAATTATCGGTGCGAAG+CGG | 0.641514 | 1.1:+59358272 | None:intergenic |
| AGGTATGTGAGTAGTGACTT+TGG | 0.648824 | 1.1:-59351096 | MS.gene52824:CDS |
| CAGCAGGAGAAATGAGACTG+AGG | 0.649017 | 1.1:-59348905 | MS.gene52824:CDS |
| ACAAGACCATCAGCTGCCAA+GGG | 0.656374 | 1.1:+59353727 | None:intergenic |
| GGATATGCTATGGCATGGAA+AGG | 0.659767 | 1.1:+59351459 | None:intergenic |
| GTTCAAGTATGCATCAGGGA+AGG | 0.661650 | 1.1:+59355785 | None:intergenic |
| CTGCCAAGGGATCAATGCGG+AGG | 0.661848 | 1.1:+59353740 | None:intergenic |
| CCTATTCCAATCAAGCAGGA+GGG | 0.662777 | 1.1:-59351237 | MS.gene52824:CDS |
| TCAAGTAATAGCGAATTGCA+AGG | 0.664537 | 1.1:+59357887 | None:intergenic |
| CCATGAGATGTGACAATGGG+TGG | 0.665060 | 1.1:+59351070 | None:intergenic |
| ATTCCAATCAAGCAGGAGGG+AGG | 0.666622 | 1.1:-59351234 | MS.gene52824:CDS |
| TATGCCTTGAGTCCAAATGA+TGG | 0.671518 | 1.1:-59350915 | MS.gene52824:CDS |
| CAAGGTTCAAGTATGCATCA+GGG | 0.675585 | 1.1:+59355781 | None:intergenic |
| GCAGTGTGTCTGTTGTTGGG+AGG | 0.677864 | 1.1:-59351671 | MS.gene52824:intron |
| AAATTGTTAATGCAGGAACA+TGG | 0.679005 | 1.1:-59354071 | MS.gene52824:intron |
| GGAGCAGGATGGCTAAATGG+TGG | 0.680480 | 1.1:+59351258 | None:intergenic |
| CCTGGTAATAAATTGCACCA+AGG | 0.681422 | 1.1:+59358246 | None:intergenic |
| GGCATACAGTTGTTACCTCG+AGG | 0.683086 | 1.1:-59356269 | MS.gene52824:CDS |
| TTTCAGTGGACATGTAGAGG+CGG | 0.686039 | 1.1:-59353258 | MS.gene52824:intron |
| AGCCAACAAGCTGAGAGTGG+TGG | 0.690346 | 1.1:+59358767 | None:intergenic |
| GATCCCATGAGATGTGACAA+TGG | 0.693395 | 1.1:+59351066 | None:intergenic |
| GTTGTATACGCAATGCCTCG+AGG | 0.703270 | 1.1:+59356254 | None:intergenic |
| GCTGATGGTCTTGTAAACAG+AGG | 0.706829 | 1.1:-59353718 | MS.gene52824:CDS |
| ATACGGTGCAGATAATCCAG+TGG | 0.709165 | 1.1:+59354006 | None:intergenic |
| TGATTGGAATAGGAGCAGGA+TGG | 0.709742 | 1.1:+59351247 | None:intergenic |
| GCACAACAGAAAGAATGTGG+AGG | 0.713802 | 1.1:-59351031 | MS.gene52824:intron |
| TCGTTTACAAAGTAGCAATG+CGG | 0.715211 | 1.1:+59349338 | None:intergenic |
| TCTGAAAGATGTTGGCAGAG+TGG | 0.720915 | 1.1:-59354353 | MS.gene52824:CDS |
| TTGTTAATGCAGGAACATGG+TGG | 0.744612 | 1.1:-59354068 | MS.gene52824:intron |
| ATCAGATTATGGTCTCCCTG+AGG | 0.756050 | 1.1:-59349159 | MS.gene52824:CDS |
| CAGCTGCCAAGGGATCAATG+CGG | 0.837731 | 1.1:+59353737 | None:intergenic |
| GATAGCAAAATTGATCAACG+AGG | 0.841690 | 1.1:-59350820 | MS.gene52824:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 59348339 | 59358830 | 59348339 | ID=MS.gene52824 |
| chr1.1 | mRNA | 59348339 | 59358830 | 59348339 | ID=MS.gene52824.t1;Parent=MS.gene52824 |
| chr1.1 | exon | 59358653 | 59358830 | 59358653 | ID=MS.gene52824.t1.exon1;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59358653 | 59358830 | 59358653 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59358247 | 59358392 | 59358247 | ID=MS.gene52824.t1.exon2;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59358247 | 59358392 | 59358247 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59358041 | 59358139 | 59358041 | ID=MS.gene52824.t1.exon3;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59358041 | 59358139 | 59358041 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59357876 | 59357926 | 59357876 | ID=MS.gene52824.t1.exon4;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59357876 | 59357926 | 59357876 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59357248 | 59357364 | 59357248 | ID=MS.gene52824.t1.exon5;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59357248 | 59357364 | 59357248 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59357025 | 59357081 | 59357025 | ID=MS.gene52824.t1.exon6;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59357025 | 59357081 | 59357025 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59356171 | 59356290 | 59356171 | ID=MS.gene52824.t1.exon7;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59356171 | 59356290 | 59356171 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59355767 | 59355829 | 59355767 | ID=MS.gene52824.t1.exon8;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59355767 | 59355829 | 59355767 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59355589 | 59355667 | 59355589 | ID=MS.gene52824.t1.exon9;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59355589 | 59355667 | 59355589 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59354693 | 59354802 | 59354693 | ID=MS.gene52824.t1.exon10;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59354693 | 59354802 | 59354693 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59354330 | 59354383 | 59354330 | ID=MS.gene52824.t1.exon11;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59354330 | 59354383 | 59354330 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59354164 | 59354231 | 59354164 | ID=MS.gene52824.t1.exon12;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59354164 | 59354231 | 59354164 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59353976 | 59354078 | 59353976 | ID=MS.gene52824.t1.exon13;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59353976 | 59354078 | 59353976 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59353598 | 59353800 | 59353598 | ID=MS.gene52824.t1.exon14;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59353598 | 59353800 | 59353598 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59353178 | 59353274 | 59353178 | ID=MS.gene52824.t1.exon15;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59353178 | 59353274 | 59353178 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59351618 | 59351689 | 59351618 | ID=MS.gene52824.t1.exon16;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59351618 | 59351689 | 59351618 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59351429 | 59351505 | 59351429 | ID=MS.gene52824.t1.exon17;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59351429 | 59351505 | 59351429 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59351213 | 59351329 | 59351213 | ID=MS.gene52824.t1.exon18;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59351213 | 59351329 | 59351213 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59351032 | 59351116 | 59351032 | ID=MS.gene52824.t1.exon19;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59351032 | 59351116 | 59351032 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59350779 | 59350946 | 59350779 | ID=MS.gene52824.t1.exon20;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59350779 | 59350946 | 59350779 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59349510 | 59349620 | 59349510 | ID=MS.gene52824.t1.exon21;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59349510 | 59349620 | 59349510 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59349328 | 59349414 | 59349328 | ID=MS.gene52824.t1.exon22;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59349328 | 59349414 | 59349328 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59349095 | 59349222 | 59349095 | ID=MS.gene52824.t1.exon23;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59349095 | 59349222 | 59349095 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59348901 | 59348983 | 59348901 | ID=MS.gene52824.t1.exon24;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59348901 | 59348983 | 59348901 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59348584 | 59348689 | 59348584 | ID=MS.gene52824.t1.exon25;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59348584 | 59348689 | 59348584 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| chr1.1 | exon | 59348339 | 59348477 | 59348339 | ID=MS.gene52824.t1.exon26;Parent=MS.gene52824.t1 |
| chr1.1 | CDS | 59348339 | 59348477 | 59348339 | ID=cds.MS.gene52824.t1;Parent=MS.gene52824.t1 |
| Gene Sequence |
| Protein sequence |
