AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53451

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53451.t1 MTR_5g033720 97.476 317 7 1 1 317 1 316 0.0 647
MS.gene53451.t1 MTR_1g017080 55.691 246 104 2 67 309 113 356 1.05e-85 263
MS.gene53451.t1 MTR_2g024390 54.894 235 104 1 73 307 117 349 8.84e-82 252
MS.gene53451.t1 MTR_1g084060 52.549 255 105 4 66 310 12 260 2.48e-81 248
MS.gene53451.t1 MTR_1g084060 52.549 255 105 4 66 310 12 260 3.52e-81 249
MS.gene53451.t1 MTR_5g085860 54.430 237 93 4 76 307 49 275 1.15e-73 229
MS.gene53451.t1 MTR_2g461240 35.366 246 134 5 74 295 95 339 5.92e-35 130
MS.gene53451.t1 MTR_1g012960 34.400 250 133 6 74 295 174 420 1.73e-33 128
MS.gene53451.t1 MTR_5g011070 33.594 256 131 5 73 295 27 276 5.24e-33 124
MS.gene53451.t1 MTR_3g106400 33.452 281 148 9 51 295 78 355 6.74e-32 122
MS.gene53451.t1 MTR_3g106400 33.452 281 148 9 51 295 78 355 7.72e-32 122
MS.gene53451.t1 MTR_3g106400 33.452 281 148 9 51 295 78 355 1.33e-31 122
MS.gene53451.t1 MTR_4g116545 33.468 248 136 6 74 295 71 315 6.85e-31 119
MS.gene53451.t1 MTR_4g116545 33.468 248 136 6 74 295 71 315 1.07e-30 118
MS.gene53451.t1 MTR_4g019450 54.545 55 25 0 243 297 367 421 8.41e-13 69.3
MS.gene53451.t1 MTR_8g078480 31.818 176 100 7 131 297 473 637 8.80e-13 69.3
MS.gene53451.t1 MTR_6g071190 33.913 115 67 4 72 185 41 147 1.13e-12 65.5
MS.gene53451.t1 MTR_8g098815 38.144 97 58 1 203 297 334 430 2.46e-12 67.8
MS.gene53451.t1 MTR_7g065050 50.909 55 27 0 243 297 312 366 5.00e-12 66.6
MS.gene53451.t1 MTR_4g051532 49.091 55 28 0 243 297 409 463 9.09e-12 66.2
MS.gene53451.t1 MTR_3g112300 37.079 89 50 3 209 297 324 406 3.27e-11 64.3
MS.gene53451.t1 MTR_3g112300 40.278 72 40 2 226 297 338 406 4.13e-11 63.9
MS.gene53451.t1 MTR_5g018860 49.091 55 28 0 243 297 531 585 4.71e-11 63.9
MS.gene53451.t1 MTR_7g106320 49.091 55 28 0 243 297 365 419 7.43e-11 63.5
MS.gene53451.t1 MTR_1g057790 34.653 101 66 0 197 297 307 407 8.61e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53451.t1 AT1G23380 65.455 330 98 5 1 317 1 327 3.13e-149 422
MS.gene53451.t1 AT1G23380 65.060 332 98 6 1 317 1 329 2.64e-147 417
MS.gene53451.t1 AT1G70510 62.305 321 105 5 1 316 1 310 4.05e-136 388
MS.gene53451.t1 AT1G70510 61.538 325 105 6 1 316 1 314 6.11e-134 383
MS.gene53451.t1 AT4G08150 54.772 241 105 2 73 309 134 374 3.53e-84 259
MS.gene53451.t1 AT1G62360 54.852 237 103 2 73 307 121 355 5.60e-78 243
MS.gene53451.t1 AT1G62990 33.603 247 140 6 73 295 29 275 5.52e-34 126
MS.gene53451.t1 AT5G11060 34.413 247 134 6 74 295 124 367 8.93e-34 128
MS.gene53451.t1 AT5G25220 34.553 246 135 6 74 295 160 403 3.00e-33 127
MS.gene53451.t1 AT4G32040 34.981 263 127 8 66 295 111 362 3.02e-33 126
MS.gene53451.t1 AT4G32040 34.981 263 127 8 66 295 111 362 3.65e-33 126
MS.gene53451.t1 AT5G25220 34.553 246 135 6 74 295 160 403 4.54e-33 127
MS.gene53451.t1 AT5G41410 39.130 92 54 1 208 297 361 452 1.86e-12 68.2
MS.gene53451.t1 AT5G02030 52.727 55 26 0 243 297 355 409 2.24e-12 68.2
MS.gene53451.t1 AT1G19700 36.607 112 61 2 186 297 311 412 2.94e-12 67.8
MS.gene53451.t1 AT1G19700 36.607 112 61 2 186 297 311 412 2.94e-12 67.8
MS.gene53451.t1 AT1G19700 36.607 112 61 2 186 297 311 412 2.94e-12 67.8
MS.gene53451.t1 AT1G19700 36.607 112 61 2 186 297 311 412 2.94e-12 67.8
MS.gene53451.t1 AT1G19700 36.607 112 61 2 186 297 311 412 2.94e-12 67.8
MS.gene53451.t1 AT2G23760 38.235 102 55 4 201 297 387 485 3.32e-12 67.4
MS.gene53451.t1 AT2G23760 38.235 102 55 4 201 297 387 485 3.32e-12 67.4
MS.gene53451.t1 AT2G23760 38.235 102 55 4 201 297 387 485 3.32e-12 67.4
MS.gene53451.t1 AT2G27220 50.909 55 27 0 243 297 235 289 4.01e-12 67.0
MS.gene53451.t1 AT2G27220 50.909 55 27 0 243 297 235 289 4.01e-12 67.0
MS.gene53451.t1 AT2G27220 50.909 55 27 0 243 297 253 307 5.23e-12 66.6
MS.gene53451.t1 AT1G75430 49.091 55 28 0 243 297 209 263 1.37e-11 64.7
MS.gene53451.t1 AT4G36870 30.435 207 119 10 99 297 370 559 1.70e-11 65.5
MS.gene53451.t1 AT4G36870 30.435 207 119 10 99 297 370 559 1.70e-11 65.5
MS.gene53451.t1 AT2G35940 33.858 127 77 4 172 297 326 446 2.06e-11 65.1
MS.gene53451.t1 AT2G35940 33.858 127 77 4 172 297 326 446 2.06e-11 65.1
MS.gene53451.t1 AT2G35940 33.858 127 77 4 172 297 326 446 2.06e-11 65.1
MS.gene53451.t1 AT4G34610 49.091 55 28 0 243 297 321 375 4.57e-11 63.9
MS.gene53451.t1 AT4G34610 49.091 55 28 0 243 297 321 375 4.57e-11 63.9
MS.gene53451.t1 AT4G34610 49.091 55 28 0 243 297 321 375 4.57e-11 63.9
MS.gene53451.t1 AT4G34610 49.091 55 28 0 243 297 321 375 4.57e-11 63.9
MS.gene53451.t1 AT1G75410 38.824 85 51 1 213 297 324 407 5.58e-11 63.5
MS.gene53451.t1 AT1G75410 38.824 85 51 1 213 297 324 407 5.58e-11 63.5
MS.gene53451.t1 AT2G16400 49.091 55 28 0 243 297 292 346 5.95e-11 63.5
MS.gene53451.t1 AT2G27990 50.909 55 27 0 243 297 431 485 6.85e-11 63.5

Find 74 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAATAAACTTGCAATTTC+AGG 0.099347 5.3:-22826286 None:intergenic
TCCCGAGCTTGATGAATTTA+TGG 0.187854 5.3:+22826375 MS.gene53451:CDS
TCCTGAAATTGCAAGTTTAT+TGG 0.199892 5.3:+22826285 MS.gene53451:CDS
CGCAAATTTGGAAGTCATAT+TGG 0.217603 5.3:+22833950 MS.gene53451:CDS
GCTTCAGTTCCAACTGCTTC+TGG 0.217937 5.3:+22826620 MS.gene53451:CDS
TCATATTGGTACTTTGAAAT+TGG 0.238802 5.3:+22833964 MS.gene53451:CDS
TCGCCTCCTTCAAGCTTATA+TGG 0.243547 5.3:+22826136 MS.gene53451:CDS
ACCAATTGTTAATTTGCTTC+TGG 0.259235 5.3:-22834212 None:intergenic
AATGTTTGTCTTGCTTCTTT+TGG 0.292821 5.3:-22834019 None:intergenic
GTCGTTGTTTCCACGTGTTT+TGG 0.333962 5.3:+22826346 MS.gene53451:CDS
TGAAGGAGGCGAGGGTAATA+AGG 0.352087 5.3:-22826125 None:intergenic
TTAATCAAAGAAAACGTCAT+TGG 0.361817 5.3:+22834238 MS.gene53451:CDS
ATTGAGTTGGCTAAGTCAAC+AGG 0.362593 5.3:+22834183 MS.gene53451:CDS
GATTCTCCGGTGTCATCATC+AGG 0.373798 5.3:-22825935 None:intergenic
ACCAGAAGCAAATTAACAAT+TGG 0.400017 5.3:+22834211 MS.gene53451:CDS
AACGTTGTAGCTTCGTCAAA+AGG 0.422258 5.3:-22826542 None:intergenic
AGATGATGGTGGTGCATCTT+CGG 0.425255 5.3:+22833844 MS.gene53451:intron
TCGCCGTATTCCACTGTTGT+TGG 0.437685 5.3:-22825906 None:intergenic
GGCAATCCATATAAGCTTGA+AGG 0.441233 5.3:-22826142 None:intergenic
CGGTTTCTTTCTGAAGAATG+AGG 0.456559 5.3:+22834309 MS.gene53451:CDS
GGTGGAATGTTCACTACAAA+TGG 0.464796 5.3:+22834050 MS.gene53451:CDS
CAAGACAAACATTACTTCAA+TGG 0.465456 5.3:+22834029 MS.gene53451:CDS
TGAAATTGCAAGTTTATTGG+AGG 0.470197 5.3:+22826288 MS.gene53451:CDS
ACCAACCGAATTCCTATGTT+CGG 0.480437 5.3:+22826008 MS.gene53451:CDS
GACCATAAATTCATCAAGCT+CGG 0.486176 5.3:-22826377 None:intergenic
CGGATCCGAACATAGGAATT+CGG 0.490352 5.3:-22826013 None:intergenic
AAGATCGTGAGCTCAAAGAT+AGG 0.491803 5.3:+22833921 MS.gene53451:CDS
GAAATATTTACCGTAGGGTA+AGG 0.498999 5.3:-22834073 None:intergenic
AGCTTATATGGATTGCCAAA+AGG 0.501841 5.3:+22826148 MS.gene53451:CDS
CTCAGTCATCTCTGCACTGG+TGG 0.506127 5.3:+22826587 MS.gene53451:CDS
CTTCGTCAAAAGGTCGAGTA+AGG 0.512678 5.3:-22826532 None:intergenic
ACCATAAATTCATCAAGCTC+GGG 0.523273 5.3:-22826376 None:intergenic
GAAAATTGCATATTCTCAGA+TGG 0.531157 5.3:-22834264 None:intergenic
GTCGGGAAGATTAGATTCTC+CGG 0.538481 5.3:-22825948 None:intergenic
ATGTTCAAGATGGACAATCA+AGG 0.539011 5.3:+22833894 MS.gene53451:CDS
AAATTCATCAAGCTCGGGAT+CGG 0.546306 5.3:-22826371 None:intergenic
GGAAACTGTTGTAGTCCGTC+GGG 0.552292 5.3:-22825965 None:intergenic
GTACCAACAACAGTGGAATA+CGG 0.552491 5.3:+22825903 MS.gene53451:CDS
ATATCGTCGGATCCGAACAT+AGG 0.553879 5.3:-22826020 None:intergenic
CTACAAATGGCCTTACCCTA+CGG 0.555178 5.3:+22834063 MS.gene53451:CDS
CAACTCAGTCATCTCTGCAC+TGG 0.557500 5.3:+22826584 MS.gene53451:CDS
AGGAAACTGTTGTAGTCCGT+CGG 0.558704 5.3:-22825966 None:intergenic
CGGATCCGACGATATCTTCA+CGG 0.574581 5.3:+22826028 MS.gene53451:CDS
ACAAACAAACCAGAAGCAGT+TGG 0.575932 5.3:-22826629 None:intergenic
CGACGATATCTTCACGGCGG+CGG 0.576617 5.3:+22826034 MS.gene53451:CDS
TGTTCAAGATGGACAATCAA+GGG 0.577717 5.3:+22833895 MS.gene53451:CDS
TCCGAACATAGGAATTCGGT+TGG 0.578896 5.3:-22826009 None:intergenic
TCGGACGAAGATCTTAGTAC+AGG 0.580602 5.3:+22833863 MS.gene53451:CDS
GACAAACATTACTTCAATGG+TGG 0.584232 5.3:+22834032 MS.gene53451:CDS
GGTTTCTTTCTGAAGAATGA+GGG 0.584785 5.3:+22834310 MS.gene53451:CDS
GGAAGCTGATAAGATTGAGT+TGG 0.588630 5.3:+22834170 MS.gene53451:intron
GGAGATGGTGATGTTCAAGA+TGG 0.589542 5.3:+22833884 MS.gene53451:CDS
GAAGATCTTAGTACAGGAGA+TGG 0.592331 5.3:+22833869 MS.gene53451:CDS
CGCCGCAATTCAAGATGACG+TGG 0.594449 5.3:+22826067 MS.gene53451:CDS
AATTCGGTTGGTTGATGAAG+TGG 0.597505 5.3:-22825997 None:intergenic
CACGTCATCTTGAATTGCGG+CGG 0.616624 5.3:-22826066 None:intergenic
AATAAACTTGCAATTTCAGG+AGG 0.620737 5.3:-22826283 None:intergenic
ATTCTCCGGTGTCATCATCA+GGG 0.622803 5.3:-22825934 None:intergenic
ATATAAGCTTGAAGGAGGCG+AGG 0.629211 5.3:-22826134 None:intergenic
TATAAGCTTGAAGGAGGCGA+GGG 0.630284 5.3:-22826133 None:intergenic
TCGGTTGGTTGATGAAGTGG+TGG 0.632474 5.3:-22825994 None:intergenic
TTGATGAAGTGGTGGACATG+AGG 0.640994 5.3:-22825986 None:intergenic
TGCCACGTCATCTTGAATTG+CGG 0.644950 5.3:-22826069 None:intergenic
TTTGTTTCGCTAGATGATGG+TGG 0.653500 5.3:+22833833 MS.gene53451:intron
TTGAGTTGGCTAAGTCAACA+GGG 0.662111 5.3:+22834184 MS.gene53451:CDS
CAAATCCCTGATGATGACAC+CGG 0.663407 5.3:+22825929 MS.gene53451:CDS
AATCCATATAAGCTTGAAGG+AGG 0.666838 5.3:-22826139 None:intergenic
CGCCGCCGTGAAGATATCGT+CGG 0.671280 5.3:-22826033 None:intergenic
GTTCAAGATGGACAATCAAG+GGG 0.671513 5.3:+22833896 MS.gene53451:CDS
GGAGAATCTAATCTTCCCGA+CGG 0.681800 5.3:+22825950 MS.gene53451:CDS
ACGTTGATGGAAGAAATGTA+CGG 0.686623 5.3:+22825879 None:intergenic
GTACGGAGTACCAACAACAG+TGG 0.698660 5.3:+22825896 MS.gene53451:CDS
GGGATCGGCTCCAAAACACG+TGG 0.709131 5.3:-22826356 None:intergenic
ATCCGACGATATCTTCACGG+CGG 0.757078 5.3:+22826031 MS.gene53451:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr5.3 gene 22825885 22834329 22825885 ID=MS.gene53451
chr5.3 mRNA 22825885 22834329 22825885 ID=MS.gene53451.t1;Parent=MS.gene53451
chr5.3 exon 22825885 22826169 22825885 ID=MS.gene53451.t1.exon1;Parent=MS.gene53451.t1
chr5.3 CDS 22825885 22826169 22825885 ID=cds.MS.gene53451.t1;Parent=MS.gene53451.t1
chr5.3 exon 22826274 22826396 22826274 ID=MS.gene53451.t1.exon2;Parent=MS.gene53451.t1
chr5.3 CDS 22826274 22826396 22826274 ID=cds.MS.gene53451.t1;Parent=MS.gene53451.t1
chr5.3 exon 22826494 22826641 22826494 ID=MS.gene53451.t1.exon3;Parent=MS.gene53451.t1
chr5.3 CDS 22826494 22826641 22826494 ID=cds.MS.gene53451.t1;Parent=MS.gene53451.t1
chr5.3 exon 22833846 22834084 22833846 ID=MS.gene53451.t1.exon4;Parent=MS.gene53451.t1
chr5.3 CDS 22833846 22834084 22833846 ID=cds.MS.gene53451.t1;Parent=MS.gene53451.t1
chr5.3 exon 22834171 22834329 22834171 ID=MS.gene53451.t1.exon5;Parent=MS.gene53451.t1
chr5.3 CDS 22834171 22834329 22834171 ID=cds.MS.gene53451.t1;Parent=MS.gene53451.t1
Gene Sequence

>MS.gene53451

ATGGAAGAAATGTACGGAGTACCAACAACAGTGGAATACGGCGACAAATCCCTGATGATGACACCGGAGAATCTAATCTTCCCGACGGACTACAACAGTTTCCTCATGTCCACCACTTCATCAACCAACCGAATTCCTATGTTCGGATCCGACGATATCTTCACGGCGGCGGAATCTTCTTCCGCCGCAATTCAAGATGACGTGGCATCTAATATCATGAAAGCTAAAATTGCTTCTCATCCTTATTACCCTCGCCTCCTTCAAGCTTATATGGATTGCCAAAAGGTAAATTAAGTTTGTTATTAGAATCATCATATCTGATCGTAACTGTAACTACTCGGCAACGCGCGGGTCTCAATCTAGTTTAACGAATTTGTATAATTGTTTAGGTTGGTGCTCCTCCTGAAATTGCAAGTTTATTGGAGGAGATTCAAAGAGAAAACGATATGTGTAAAAGAGACGTCGTTGTTTCCACGTGTTTTGGAGCCGATCCCGAGCTTGATGAATTTATGGTCCCCCCCTTAAATCCTTTCTTTCTTTCTTTTTATTTTATTGAAAATAAATAAATTAAATGCAACTTTATATTGATGTTGATTATGGTTGTTTCAGGAAAGTTATTGTGACATGCTTGTGAAGTATAAATCAGACCTTACTCGACCTTTTGACGAAGCTACAACGTTTCTGAACAAGATCGAAACGCAACTCAGTCATCTCTGCACTGGTGGTGCTGCTGCTGCTTCAGTTCCAACTGCTTCTGGTTTGTTTGTTTTTCTTCTCTCTCTTTCTGTTAGTTTTGGAACTCGATGTTTTTTTGTTGATAACTGTGCTTTGATTCTTATCCTCTTGTGGATTAGTTTTTTTGTTTACCTTTAACTATTTGTTTTTGTGTGATTTGAGAACCTAAATAATATACACAGTATAGTTTAATAATTACTACTACTATTAAACAAGATGAATACACAGATTTCCAAGTAAATCAAGGAAAATAATTAACAAAAATAGAAGAAAGGTAAACCCTAATAATACATCCACATCACACAGATACACACATATCCTCTTTCTATGCGTGTGCAGTTTTTTTGTTTTTGGGTCAAATTTTGTGTGCAGTTATAATGTGAGTTTTAATGTTTTATGGAATGCTTTTCAGTTTTCACTGTTAAACCGTAACGTAATCTGTGATGTTTGTTTTTGTTATAGGAAAAGTTTGTGGTGTTTGGTCATTCAAACCTTTCTTCTTTTACAGTTACACGGGTCTTTAATTACACTAGAGATTTTTGTCTTGTTTGTACTTTTGAGTTTTGAGTAGTTAGCATCACTACTCACACTTCATTTCTTCATGGTTTTCATATTTTTTATTTGTTTTATTACTAGCTATCTGCTTCATCCTCGTGAATAATGTTCAGTTTTTATGAACAACAAGGACTTTCTAACTTGACATTGCAAACAGTGGCCTAGATTCATTCAATACACTGACCAAATTTTCTTAAATGGCTCATTTGCTTCTTGCATCTATTACCTATCAAATTGTTTCTTCTTTTATTTCTTTATACTATAGTTTTAATTTTGTATGCTTTCCAGCTCATGGACACACGTATCATGCTTAATCTGATAAAGTTTCTGTTAACAAAATTTATACAGAAAATACATTCAATGAAGTAAGTTGGTTTCTATTCATAAATCATGACATACTCAGAAAATACTTCTTGTGTGCTGTCTATCTTTTGTTGGTTTCTATGTAAGTTAAGGAAACCAATGTTGCCGTGATTTTTTTTTTCTTCAAAATGATATTGTTTTTTTTATTAGCGTAATTCGCTTATGTTCATATTTGGTATCATTGTGAGTTTGATTGCACCACGGTGAGCCATTGTGATTTTGCAAAATCATAGTGGTTCGACGTGATACAAAACCTACATATTACAATCAAATTGTTGTGTGATAATATTATGTTCAAGTAAAACTTTTTTATGTTCATGAAAAGAAGAGCATTGCTCAGGCTTGAATTGTTGTCGGGCTTTGGTAGTAGAATGCAGCAGAAAAAGTTCAAAATTTGTTGTAATTGAGAACATTGAAATTTACATGTTCTTCAAATGGTACATGTAACATCAACATGCAATTGTTGCGAGATTTTTTGTGATTTGTTTTTATTTGTTGTAGTTGGTGTTTTCTTGTTTGGTGCGGTTGTTTGGTCACCTATGGTGTTACTCAAACCATGGGCATTCAATGTTTTTCTCTTATATATAATATCTATATTAAAAAAGAACATCAACATGCAATTCATTTATGTGAATTAGTGTTTGGCCTAGAAACATCCCTTTTAAGGTGTGTGCCAACTTCATTTATACTTGCATTGCATCTTTTTTTTTTTTGAGTAATTGCATTGCATCTTTCCTATTGAGCCAACAAATCTTCTCATTCAAAATTCATATTTTTATTTCTCTAAAATATTTACATATTTTGATAGGATATCACGGTTTTAATACACAAATACTCAAATACTGGTAGTACTTATTTGGTACGAAATTTTTCATGAGATAATCTATTTTGCATGAAGCTTAATTGTAATTTCATAAATTCATTTGGTGATGTATCACATGATAAATATAATAACCTTCCAAAGCTCCTACATTTTGGATTAGATGAACACAACATGATCTGACAGTAATGGCATGCAATGATTCTACTTTCATACTTTATTAATTATATAATGTATCGAGTTCCAATAGACCACAAGGGATCCATGCTGTTGTTGGTTGATAAAAAAAAAATAGCCATTGGGAATTGTCTAAAGGGCTGATAGTCTGTAGTATGTAGCTAAAGTAATGTCAGATTTTCTTTAAATGAGTCATTTAGACAATTGTAGTATGAGGAATTGTCTAAAGGGCTGATAGTCTGTAGTATGGACCACATACATCAATTATTGAATAAACTTAAAAAGTGAAAAGTTTCTTACATTTGAGACCGGAGGGAGTAAGTGCAAAGCAGTTTGGGTTGGTAAGTTCATGGTTTTAAATTGTGGTTGCAGTTACGCCGCAAACCCTTATATCGCGGAAAAATACTGTTGAAATTTTGGTTGCGGAGGCCTTAAAATCCGTTATATTGTGGTCGCACTTTAAAACCATGGATACAATTAGAAAAACAAATAAGCTAAAAATTGTAGTCAGGTTTAAAACCATGGATACAATTAGAAAAACAAATAAGCTTAAAATTGTAGTCAGGTTTTTCTGTTATTACTATATTTGAAGGAAGGTTTTTTAATGTATTGTGAACTAAGGAGTCCAACTGATTTAGAATTTGATGGTTTGCTTCTTATAATGATATATCTTCCAGATCTTAACGAATTTCTTATTATAAAAACTATATCTTACACATGCATATGGGAGTATATTTATTTTTGTAGTTATATATAAACTTTAATTTTATAGATTTTATTTTGGTTTTATGGATTCTAGAATATATGTTAACAATTCCCATGCCCATTTGCTGATTATGGAAGCTGGCACAGATGTATTAAATATATCAATTTTGAAAGAATATTTTATGCTGTAAAGCATGGTCACTGGTCAGGTTGGTTATGATTTGAAAATATTGTTGTTTATGTTTGAAAGTACAAATTACCTTTTCCGTTTTTAGCTGTGATGTTTTTAATTAGTAAATTAGTACTTTAGATTGCATCATAAACGGATTCTCCTTCATCATCCCTTTTACAAATATAAATTCTTAATTTTTGCTTCATAATCAAATTGTTGATAGGAGGATATGAAATCCTTTCTTTTTAATTTATTCTTGTTGTCCTTCTGCTGCAATCAGGCGTTAAATTGGATCTTGTGTTTCTAAGAAGCAAGTTTTTAGGTGTTTAGGGGGTATAAGTTCTTTTGATGGTTAGTTTGGTGGTTAGAGTGTTCAGTGATGAATAATTTAAGTGTTGCATCCGTACCCTAAATGACATTTGTTAGTATGATCCATACTAATAATACCGATATTTTTCTGCTATGTTTATCAACTTGTGCTTTTTATTGATCTTCCCCAACTGCTATGAGAAAATCTCTTGGAATTTATGTCGTGAAATAAATGATGTCATCTTCAGTCTTTATTTTGAATGTCTATACCCGAAGACACTGGTTAACATTTTTCTTATAATTATAGTACGTGAAATACTTAGAATGACATGGTTGATTTTTAGTGTTCATTAGACCTCTAAAGTGACTTCAATATTTTGATTTTAATTTTGATTTGTTTTTTTTTTTTGTGTGTGTAATATATAACTTTTAGAAGTTTGGGTTATTTTAAGTTTAAATAATGACAACTAGAATCACCTCAATGTGAATCCTAAAGTGTTTCTTTTCCCTTTATGACCGAAGACATGTTTAAGTACAAAAAAGTGTACTACATCCAGGCCATATATATTATAAAGTGTAGCTTGAGATTAAAGTTGATGATTGTATATAGGAAGAAAATAGAGGATTGAAGTGAAAAGGAATAATAAACATTAAATTTTGGTAGTGATAGAGTCAATATAGTTGGAGTCACATTCTCAATAGAAATGCACCATGTTTTATTATATTAAAAAAAATAACAATAAACTATAAAGTTCTTTGCTTGAGCCTTATCTCTTACAAGGACATTTGAATGTAAACATGTTGGCTGTTCATTACTTTATGTTGTGGCATATATAGAATTGCAAATTGGAAGGTGGGATGTTTATTGGGTGGCACTTTGGTGCCATTAATGGCATGTGGCACCATGCCATTAGATTAGGGCCACACATTGCCTCAAGTGTGTGTGTGTGATGTGTGTGTTGGGTGTCATCAATGGCCATGATTTAATGGCACGGTGCCATTAATGGCACCCAAGTATTGTCCATGTTTATTAGTGTCATTGCGAAATGGGTAAGAGATTGGACGAATTGACATCGAGTATAAAGTAACAATATAGATCATATAAGAGAATATGTTTGTAGCATTGGAGTGAAACAAGAGTTGCTACATCTTACTCAATGATTCTAGCAACAAATATGTTTACGAAGAGAGAATATGTAAATAGTGGGGTCCATTTGAGTTTTTAAAGTTGTAGGCTGTTGGAGTAAAAAATTTGGACAATTCTTAATAGTGATATGCTGGTTATGGCCCACTAAAAATGGAGTAGATATTTCTTCATTTTTCTTAAGGTCAAGTTAGACAAGTCTGTGCACAAGATTATTTCTACAATTAGATTAAAACCCTTTATCGATATAATGTGACTTGATTGAATGATAGAAAATAAACTTGTATATCTTGTTAGACCGACAAAGTTTTTTGAGATCTCCATTACATTAGTTTTACCGTATAAAATTCTAATCAATGCTACCAAGTTATATTTAAATTGTTTATTCACTCTAGAATTATAGTAATAGACACTTTAAAAATGGAGAGGATCCTTATTCCTTACCCTAAAAATATGGTTAGAACCAAATTTTGGTTTTAGCATATGCGTAGATCCTAGAAAAGAGTAGACTGGTTTATTCTGTGCAGTCTGTTTATATACGTTTTGCTATATTGTCTTTTTTTTGGGTTAATTTCCTTTTACTTGTTTAAATTTTTGTCTATTCCTATGGGCTGAAATTGAAGAACCAAATAAACCCTAGGGTTTTTTTGGTTGGTAAAGAATAAACCTTAGGTTTTACTAGAAGGCTAATGATTTCACTTTTAGAACTAAAATTACTACACCCTCCGGTCACTATTATAAGCAAAATTTCATTTTTTAGGTTCATTCAATAAATGATGTATGTGATCTATTTAATGGACTACATTCATCATTTATTAAATGAACCTAAAAAGTAGATTTTTGCTTATAATAGTGATCAGAGGGTGTACAAGTTACAATTACAATTACAAGGTCTCGGATCTCCAAAATAACTTGGACTCATCAGAAACATGGAATGAAACAAGAATGAAAACCTTGGAAGCTGTATAATAATAATGCAGTTTGGTAGTATTATATATATTTAACTAATAGGAGGAGGAAGTGATTCTGATGAATACTGAAAACTGATTATAGAACCTTTTACAAAACAATCCCGTGTACAACGGGTGATATACATTACAAGATTATTAAAACTTTCACATGCACTCCCGGAATTTAAGCTGTTTTCTACAGCAGCTTTTACGTTTGAAAGAACGTTCAATGCCATGAATATCTTCCCCCCCACGCACTCTGCCTTACCCTGTCTTTGTGCTTTGTTTTGTTATTGTTCACTATGAGCTTCTGCTATTAACCTTTAGACCAATGGAGGGACTATAACACTTGTCGAATTGTAAATGCACTTTCTTTTATAGGGTCCCTCTTTGGAAAAGAAACCTATCTTATTCTCTTGATGGTAGAGTATAGTTTTGGTTGGACATTTGCTTTTGGGAACAATTTTCGTACTCATGCTACTACTGGCATGTTACAAGGTCTTCATTTCACAAATTAAATGTGAGCATAGTTTAAATAAGTAAATAAATAAATAAATAAATGTACTTAGTTTGAACAAATTTTAATGGTAAATTTGAATGAGGAAATTCATGGTATGGTAAATTGGTACGTACAAATTGATTGAAATTGAATGTGAACTTGGACTTGGAGTGATTCTGGATTGGGACATAGTAGTGACAATGGATGGAACCAGGCACTGACACTGATGTTTTCTTTTTCTTTACACTTTTTTCTTTCTTTTCTTTCCAACCTAGGTCTGCTATGATCTGACGTGTACAGTGTACTCTCATGCGACGGTCAAATCCTTGTCGGATTGGGTGGGTGTCAGAAGCAACTTATGGCTACTCGTGGGCTACTACTAGCCCTCTAATTAAACATTATTCCATATTTAAGATAAGTTAGTCAGAAATGATAGAGTTGCAAAAGTTTATCCCTAGATTAATTAATGTTTAGGAAAAAATATACTCCCTCGGGCCTTAAATATAAGAAAAAATTCTTTTTTTAGATTCATTGAATAATTAATGTATATGCTTTACAATGTAGATCAGATACATTAACTACTCAATGAACTTAAAAAATGAATATTTTCTTATATTTGAGACCGGATGGAGTAATTAGATACTCTCTCCGGTCCTTATTATAAGAAACAATTGACTTTTTTGGTTCATAGAATAATTAATGTATCTTATTATAAATATAGACTAGATACATCATTTACACAATGAACCTAAAAAGTCAACTTTTTCTTATAATAAGGACAGGAAGGAATATTAGGATTCACCATTTAAGATAAGCTGGTCAGATTATTTTTAAAAAAAAAGTCGGTAATAATTGTGGCAAAAGTACTACAGTAGATTGGCTTTGTATTTTAGGTTACAAAGTTATTTAGATTTAGATAGAAGAACAATTATTGTATAGCAAACTTGAAGAAGAAAAATGGAACAATTCTTACTTAGTACGGCCAAATCAAATTAATTGGACACACATAAATATGAAACATTAGTTTATGGTTTATAAATTAAAGTATTTTCTCCAGATTAATATTTATACAAAGTTTATAAATTAATATTAAGAGTCTTCAACTAATTTATAAATATATTAATAGATATTAAGAGATTGTCTTTAATTAATTATTAGATTAAATTAACACAGTTTGCTTTTTTAATGGTTCATATGGACCTTCAAAAAAAAAAGAACTCAAGGTAAACTTTTACAATTTTTTAATAATAATGTAATGTAAAAATAGAGTGTATTTTGCATAATGTAATGTTTTATTAAAAATAGTAAAAGTTTATCTTTCGACAATGTTTTTGAGCTCCATCAGTACACCTTTTTAAGAGTATATTTTGCATAATTTTTTAACAAAGTTTTTTAAAGAAATTGTTAGCATCTTCATTTTATTTTTGCATAATCAATGGTGTTTATTTTTGTACGGTTTTAATATTTAACATAGAGATAGAAACAATTAACATTGAGCTTAATGATTTTTCCTATTTTCTTTGTTTCGCTAGATGATGGTGGTGCATCTTCGGACGAAGATCTTAGTACAGGAGATGGTGATGTTCAAGATGGACAATCAAGGGGAGAAGATCGTGAGCTCAAAGATAGGCTTTTACGCAAATTTGGAAGTCATATTGGTACTTTGAAATTGGAGTTTTCAAAGAAGAAAAAGAAGGGTAAGCTTCCAAAAGAAGCAAGACAAACATTACTTCAATGGTGGAATGTTCACTACAAATGGCCTTACCCTACGGTAAATATTTCATTTCCCATTTACTAAACTTTATACTAATAATCATTCAAAATTAACAACGAAAAATGTTTTACATGATTATGCAGGAAGCTGATAAGATTGAGTTGGCTAAGTCAACAGGGTTAGACCAGAAGCAAATTAACAATTGGTTTATTAATCAAAGAAAACGTCATTGGAAACCATCTGAGAATATGCAATTTTCTATGATGGAAAATTTTACAGGACGGTTTCTTTCTGAAGAATGA
Protein sequence

>MS.gene53451.t1

MEEMYGVPTTVEYGDKSLMMTPENLIFPTDYNSFLMSTTSSTNRIPMFGSDDIFTAAESSSAAIQDDVASNIMKAKIASHPYYPRLLQAYMDCQKVGAPPEIASLLEEIQRENDMCKRDVVVSTCFGADPELDEFMESYCDMLVKYKSDLTRPFDEATTFLNKIETQLSHLCTGGAAAASVPTASDDGGASSDEDLSTGDGDVQDGQSRGEDRELKDRLLRKFGSHIGTLKLEFSKKKKKGKLPKEARQTLLQWWNVHYKWPYPTEADKIELAKSTGLDQKQINNWFINQRKRHWKPSENMQFSMMENFTGRFLSEE