Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44340.t1 | XP_003613183.1 | 96.8 | 284 | 9 | 0 | 1 | 284 | 1 | 284 | 3.20E-149 | 537.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44340.t1 | Q84JS6 | 61.7 | 298 | 97 | 5 | 1 | 284 | 1 | 295 | 8.6e-95 | 348.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44340.t1 | G7K060 | 96.8 | 284 | 9 | 0 | 1 | 284 | 1 | 284 | 2.3e-149 | 537.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050807 | MS.gene44340 | 0.806527 | 7.88E-50 | -1.69E-46 |
| MS.gene050901 | MS.gene44340 | 0.832286 | 1.03E-55 | -1.69E-46 |
| MS.gene051201 | MS.gene44340 | 0.800256 | 1.57E-48 | -1.69E-46 |
| MS.gene051648 | MS.gene44340 | 0.830202 | 3.35E-55 | -1.69E-46 |
| MS.gene051664 | MS.gene44340 | 0.828588 | 8.27E-55 | -1.69E-46 |
| MS.gene051719 | MS.gene44340 | 0.835625 | 1.50E-56 | -1.69E-46 |
| MS.gene051720 | MS.gene44340 | 0.835227 | 1.90E-56 | -1.69E-46 |
| MS.gene052150 | MS.gene44340 | 0.824539 | 7.64E-54 | -1.69E-46 |
| MS.gene052151 | MS.gene44340 | 0.813113 | 3.02E-51 | -1.69E-46 |
| MS.gene053144 | MS.gene44340 | 0.802102 | 6.58E-49 | -1.69E-46 |
| MS.gene053563 | MS.gene44340 | 0.816607 | 5.07E-52 | -1.69E-46 |
| MS.gene053581 | MS.gene44340 | 0.820871 | 5.45E-53 | -1.69E-46 |
| MS.gene054791 | MS.gene44340 | 0.813405 | 2.60E-51 | -1.69E-46 |
| MS.gene055537 | MS.gene44340 | 0.826128 | 3.22E-54 | -1.69E-46 |
| MS.gene056005 | MS.gene44340 | 0.83805 | 3.62E-57 | -1.69E-46 |
| MS.gene05894 | MS.gene44340 | 0.815717 | 8.01E-52 | -1.69E-46 |
| MS.gene060519 | MS.gene44340 | 0.855019 | 8.35E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene53451 | MS.gene44340 | PPI |
| MS.gene44340 | MS.gene80724 | PPI |
| MS.gene44340 | MS.gene44341 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44340.t1 | MTR_5g033720 | 98.592 | 284 | 4 | 0 | 1 | 284 | 1 | 284 | 0.0 | 585 |
| MS.gene44340.t1 | MTR_1g017080 | 53.604 | 222 | 98 | 2 | 66 | 284 | 113 | 332 | 3.03e-69 | 219 |
| MS.gene44340.t1 | MTR_2g024390 | 52.582 | 213 | 99 | 1 | 72 | 284 | 117 | 327 | 1.23e-65 | 209 |
| MS.gene44340.t1 | MTR_1g084060 | 50.000 | 230 | 99 | 3 | 65 | 284 | 12 | 235 | 9.38e-64 | 201 |
| MS.gene44340.t1 | MTR_1g084060 | 50.000 | 230 | 99 | 3 | 65 | 284 | 12 | 235 | 1.37e-63 | 202 |
| MS.gene44340.t1 | MTR_5g085860 | 51.628 | 215 | 89 | 4 | 75 | 284 | 49 | 253 | 1.65e-56 | 184 |
| MS.gene44340.t1 | MTR_2g461240 | 32.917 | 240 | 128 | 6 | 73 | 284 | 95 | 329 | 1.97e-26 | 106 |
| MS.gene44340.t1 | MTR_1g012960 | 30.802 | 237 | 139 | 5 | 73 | 284 | 174 | 410 | 6.27e-26 | 106 |
| MS.gene44340.t1 | MTR_5g011070 | 31.301 | 246 | 130 | 5 | 72 | 284 | 27 | 266 | 3.22e-25 | 102 |
| MS.gene44340.t1 | MTR_3g106400 | 31.365 | 271 | 147 | 10 | 50 | 284 | 78 | 345 | 6.03e-24 | 100 |
| MS.gene44340.t1 | MTR_3g106400 | 31.365 | 271 | 147 | 10 | 50 | 284 | 78 | 345 | 8.01e-24 | 99.8 |
| MS.gene44340.t1 | MTR_3g106400 | 30.996 | 271 | 148 | 9 | 50 | 284 | 78 | 345 | 1.12e-23 | 99.8 |
| MS.gene44340.t1 | MTR_4g116545 | 29.832 | 238 | 138 | 6 | 73 | 284 | 71 | 305 | 1.79e-22 | 95.5 |
| MS.gene44340.t1 | MTR_4g116545 | 29.832 | 238 | 138 | 6 | 73 | 284 | 71 | 305 | 2.65e-22 | 94.7 |
| MS.gene44340.t1 | MTR_6g071190 | 33.913 | 115 | 67 | 4 | 71 | 184 | 41 | 147 | 9.35e-13 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44340.t1 | AT1G23380 | 64.094 | 298 | 90 | 5 | 1 | 284 | 1 | 295 | 8.64e-126 | 361 |
| MS.gene44340.t1 | AT1G23380 | 63.667 | 300 | 90 | 6 | 1 | 284 | 1 | 297 | 5.76e-124 | 357 |
| MS.gene44340.t1 | AT1G70510 | 61.592 | 289 | 96 | 5 | 1 | 284 | 1 | 279 | 6.61e-117 | 338 |
| MS.gene44340.t1 | AT1G70510 | 60.751 | 293 | 96 | 6 | 1 | 284 | 1 | 283 | 1.61e-114 | 332 |
| MS.gene44340.t1 | AT4G08150 | 52.995 | 217 | 98 | 2 | 72 | 284 | 134 | 350 | 2.78e-68 | 217 |
| MS.gene44340.t1 | AT1G62360 | 52.093 | 215 | 99 | 2 | 72 | 284 | 121 | 333 | 5.25e-62 | 201 |
| MS.gene44340.t1 | AT4G32040 | 30.938 | 320 | 167 | 12 | 6 | 284 | 46 | 352 | 3.33e-26 | 106 |
| MS.gene44340.t1 | AT1G62990 | 31.224 | 237 | 139 | 6 | 72 | 284 | 29 | 265 | 3.73e-26 | 104 |
| MS.gene44340.t1 | AT4G32040 | 30.938 | 320 | 167 | 12 | 6 | 284 | 46 | 352 | 4.29e-26 | 106 |
| MS.gene44340.t1 | AT5G11060 | 31.646 | 237 | 134 | 6 | 73 | 284 | 124 | 357 | 1.60e-25 | 104 |
| MS.gene44340.t1 | AT5G25220 | 29.831 | 295 | 165 | 7 | 32 | 284 | 99 | 393 | 1.61e-25 | 105 |
| MS.gene44340.t1 | AT5G25220 | 29.831 | 295 | 165 | 7 | 32 | 284 | 99 | 393 | 2.18e-25 | 104 |
Find 66 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAATAAACTTGCAATTTC+AGG | 0.099347 | 5.1:-24637950 | None:intergenic |
| TCCCGAGCTTGATGAATTTA+TGG | 0.187854 | 5.1:+24638039 | MS.gene44340:CDS |
| TCCTGAAATTGCAAGTTTAT+TGG | 0.199892 | 5.1:+24637949 | MS.gene44340:CDS |
| CGCAAATTTGGAAGTCATAT+TGG | 0.213196 | 5.1:+24645659 | MS.gene44340:CDS |
| GCTTCAGTTCCAACTGCTTC+TGG | 0.217937 | 5.1:+24638280 | MS.gene44340:CDS |
| TCATATTGGCACTTTGAAAT+TGG | 0.238314 | 5.1:+24645673 | MS.gene44340:CDS |
| AATATTTGTCTTGCTTCTTT+AGG | 0.247479 | 5.1:-24645728 | None:intergenic |
| ACCAATTGTTAATTTGCTTC+TGG | 0.259235 | 5.1:-24645921 | None:intergenic |
| TGAAGGAGACGAGGGTAATA+AGG | 0.358906 | 5.1:-24637788 | None:intergenic |
| ATTGAACTGGCTAAGTCAAC+AGG | 0.375983 | 5.1:+24645892 | MS.gene44340:CDS |
| GACAATCGATATAAGCTTGA+AGG | 0.382556 | 5.1:-24637805 | None:intergenic |
| GTTGTTGTTTCCACGTGTTT+TGG | 0.385565 | 5.1:+24638010 | MS.gene44340:CDS |
| TTAGATTCTCCGGTGTCATC+AGG | 0.388564 | 5.1:-24637601 | None:intergenic |
| ACCAGAAGCAAATTAACAAT+TGG | 0.400017 | 5.1:+24645920 | MS.gene44340:CDS |
| AACGTTGTAGCTTCGTCAAA+AGG | 0.422258 | 5.1:-24638202 | None:intergenic |
| ACCGGAGAATCTAATCTTCC+CGG | 0.432363 | 5.1:+24637610 | MS.gene44340:CDS |
| TCGCCGTATTCCACTGTTGT+TGG | 0.437685 | 5.1:-24637572 | None:intergenic |
| AGATGATGGTGGTGCATCTT+CGG | 0.453738 | 5.1:+24645553 | MS.gene44340:intron |
| GGTGGAATGTTCACTACAAA+TGG | 0.464796 | 5.1:+24645759 | MS.gene44340:CDS |
| TGAAATTGCAAGTTTATTGG+AGG | 0.470197 | 5.1:+24637952 | MS.gene44340:CDS |
| CAAGACAAATATTACTTCAA+TGG | 0.473319 | 5.1:+24645738 | MS.gene44340:CDS |
| ACCAACCGAATTCCTATGTT+CGG | 0.480437 | 5.1:+24637671 | MS.gene44340:CDS |
| GACCATAAATTCATCAAGCT+CGG | 0.486176 | 5.1:-24638041 | None:intergenic |
| GGAAGCTGATAAGATTGAAC+TGG | 0.489777 | 5.1:+24645879 | MS.gene44340:intron |
| CGGATCCGAACATAGGAATT+CGG | 0.490352 | 5.1:-24637676 | None:intergenic |
| AAGATCGTGAGCTCAAAGAT+AGG | 0.491803 | 5.1:+24645630 | MS.gene44340:CDS |
| AGCTTATATCGATTGTCAAA+AGG | 0.494441 | 5.1:+24637811 | MS.gene44340:CDS |
| GAAATATTTACCGTAGGGTA+AGG | 0.498999 | 5.1:-24645782 | None:intergenic |
| CTCAGTCATCTCTGCACTGG+TGG | 0.506127 | 5.1:+24638247 | MS.gene44340:CDS |
| CTTCGTCAAAAGGTCGAGTA+AGG | 0.512678 | 5.1:-24638192 | None:intergenic |
| AGGAAACTGTTGTAGTCCGC+CGG | 0.522277 | 5.1:-24637629 | None:intergenic |
| ACCATAAATTCATCAAGCTC+GGG | 0.523273 | 5.1:-24638040 | None:intergenic |
| GCCGGGAAGATTAGATTCTC+CGG | 0.538481 | 5.1:-24637611 | None:intergenic |
| ATGTTCAAGATGGACAATCA+AGG | 0.539011 | 5.1:+24645603 | MS.gene44340:CDS |
| AAATTCATCAAGCTCGGGAT+CGG | 0.546306 | 5.1:-24638035 | None:intergenic |
| GACAAATATTACTTCAATGG+TGG | 0.550926 | 5.1:+24645741 | MS.gene44340:CDS |
| GTACCAACAACAGTGGAATA+CGG | 0.552491 | 5.1:+24637569 | MS.gene44340:CDS |
| ATATCGTCGGATCCGAACAT+AGG | 0.553879 | 5.1:-24637683 | None:intergenic |
| CTACAAATGGCCTTACCCTA+CGG | 0.555178 | 5.1:+24645772 | MS.gene44340:CDS |
| CAACTCAGTCATCTCTGCAC+TGG | 0.557500 | 5.1:+24638244 | MS.gene44340:CDS |
| CGGATCCGACGATATCTTCA+CGG | 0.574581 | 5.1:+24637691 | MS.gene44340:CDS |
| TGTTCAAGATGGACAATCAA+GGG | 0.577717 | 5.1:+24645604 | MS.gene44340:CDS |
| TCCGAACATAGGAATTCGGT+TGG | 0.578896 | 5.1:-24637672 | None:intergenic |
| TCGGACGAAGATCTTAGTAC+AGG | 0.580602 | 5.1:+24645572 | MS.gene44340:CDS |
| GGAAACTGTTGTAGTCCGCC+GGG | 0.588293 | 5.1:-24637628 | None:intergenic |
| GGAGATGGTGATGTTCAAGA+TGG | 0.589542 | 5.1:+24645593 | MS.gene44340:CDS |
| GAAGATCTTAGTACAGGAGA+TGG | 0.592331 | 5.1:+24645578 | MS.gene44340:CDS |
| CGCCGCAATTCAAGATGACG+TGG | 0.594449 | 5.1:+24637730 | MS.gene44340:CDS |
| AATTCGGTTGGTTGATGAAG+TGG | 0.597505 | 5.1:-24637660 | None:intergenic |
| TAGATTCTCCGGTGTCATCA+GGG | 0.607484 | 5.1:-24637600 | None:intergenic |
| CGACAAATCCCTGATGACAC+CGG | 0.609163 | 5.1:+24637592 | MS.gene44340:CDS |
| CACGTCATCTTGAATTGCGG+CGG | 0.616624 | 5.1:-24637729 | None:intergenic |
| AATAAACTTGCAATTTCAGG+AGG | 0.620737 | 5.1:-24637947 | None:intergenic |
| TCGGTTGGTTGATGAAGTGG+TGG | 0.632474 | 5.1:-24637657 | None:intergenic |
| TTGATGAAGTGGTGGACATG+AGG | 0.640994 | 5.1:-24637649 | None:intergenic |
| TGCCACGTCATCTTGAATTG+CGG | 0.644950 | 5.1:-24637732 | None:intergenic |
| ATATAAGCTTGAAGGAGACG+AGG | 0.666970 | 5.1:-24637797 | None:intergenic |
| GTTCAAGATGGACAATCAAG+GGG | 0.671513 | 5.1:+24645605 | MS.gene44340:CDS |
| TTGAACTGGCTAAGTCAACA+GGG | 0.674143 | 5.1:+24645893 | MS.gene44340:CDS |
| AGCCGCCGTGAAGATATCGT+CGG | 0.684032 | 5.1:-24637696 | None:intergenic |
| ACGTTGATGGAAGAAATGTA+CGG | 0.686623 | 5.1:+24637545 | None:intergenic |
| TATAAGCTTGAAGGAGACGA+GGG | 0.691645 | 5.1:-24637796 | None:intergenic |
| GTACGGAGTACCAACAACAG+TGG | 0.698660 | 5.1:+24637562 | MS.gene44340:CDS |
| GGGATCGGCTCCAAAACACG+TGG | 0.709131 | 5.1:-24638020 | None:intergenic |
| GGAGAATCTAATCTTCCCGG+CGG | 0.768163 | 5.1:+24637613 | MS.gene44340:CDS |
| ATCCGACGATATCTTCACGG+CGG | 0.775616 | 5.1:+24637694 | MS.gene44340:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 24637551 | 24645941 | 24637551 | ID=MS.gene44340 |
| chr5.1 | mRNA | 24637551 | 24645941 | 24637551 | ID=MS.gene44340.t1;Parent=MS.gene44340 |
| chr5.1 | exon | 24637551 | 24637832 | 24637551 | ID=MS.gene44340.t1.exon1;Parent=MS.gene44340.t1 |
| chr5.1 | CDS | 24637551 | 24637832 | 24637551 | ID=cds.MS.gene44340.t1;Parent=MS.gene44340.t1 |
| chr5.1 | exon | 24637938 | 24638060 | 24637938 | ID=MS.gene44340.t1.exon2;Parent=MS.gene44340.t1 |
| chr5.1 | CDS | 24637938 | 24638060 | 24637938 | ID=cds.MS.gene44340.t1;Parent=MS.gene44340.t1 |
| chr5.1 | exon | 24638154 | 24638301 | 24638154 | ID=MS.gene44340.t1.exon3;Parent=MS.gene44340.t1 |
| chr5.1 | CDS | 24638154 | 24638301 | 24638154 | ID=cds.MS.gene44340.t1;Parent=MS.gene44340.t1 |
| chr5.1 | exon | 24645555 | 24645793 | 24645555 | ID=MS.gene44340.t1.exon4;Parent=MS.gene44340.t1 |
| chr5.1 | CDS | 24645555 | 24645793 | 24645555 | ID=cds.MS.gene44340.t1;Parent=MS.gene44340.t1 |
| chr5.1 | exon | 24645880 | 24645941 | 24645880 | ID=MS.gene44340.t1.exon5;Parent=MS.gene44340.t1 |
| chr5.1 | CDS | 24645880 | 24645941 | 24645880 | ID=cds.MS.gene44340.t1;Parent=MS.gene44340.t1 |
| Gene Sequence |
| Protein sequence |
