Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53929.t1 | KEH32796.1 | 90.1 | 385 | 37 | 1 | 168 | 551 | 77 | 461 | 1.00E-207 | 733 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53929.t1 | F4I933 | 66.7 | 363 | 107 | 5 | 188 | 538 | 81 | 441 | 8.2e-142 | 505.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53929.t1 | A0A072UTY1 | 90.1 | 385 | 37 | 1 | 168 | 551 | 77 | 461 | 7.3e-208 | 733.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050301 | MS.gene53929 | 0.847108 | 1.42E-59 | -1.69E-46 |
| MS.gene053836 | MS.gene53929 | 0.81485 | 1.25E-51 | -1.69E-46 |
| MS.gene05455 | MS.gene53929 | 0.828276 | 9.84E-55 | -1.69E-46 |
| MS.gene055428 | MS.gene53929 | 0.814878 | 1.23E-51 | -1.69E-46 |
| MS.gene058002 | MS.gene53929 | 0.82399 | 1.03E-53 | -1.69E-46 |
| MS.gene05811 | MS.gene53929 | 0.8179 | 2.59E-52 | -1.69E-46 |
| MS.gene058660 | MS.gene53929 | 0.801321 | 9.52E-49 | -1.69E-46 |
| MS.gene05907 | MS.gene53929 | 0.809531 | 1.81E-50 | -1.69E-46 |
| MS.gene059597 | MS.gene53929 | 0.832475 | 9.26E-56 | -1.69E-46 |
| MS.gene059688 | MS.gene53929 | 0.807026 | 6.18E-50 | -1.69E-46 |
| MS.gene059692 | MS.gene53929 | 0.815777 | 7.77E-52 | -1.69E-46 |
| MS.gene059695 | MS.gene53929 | 0.820707 | 5.95E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53929.t1 | MTR_3g006290 | 91.688 | 385 | 31 | 1 | 168 | 551 | 77 | 461 | 0.0 | 749 |
| MS.gene53929.t1 | MTR_3g006290 | 92.401 | 329 | 24 | 1 | 168 | 495 | 77 | 405 | 0.0 | 640 |
| MS.gene53929.t1 | MTR_6g077680 | 47.500 | 240 | 48 | 5 | 6 | 168 | 172 | 410 | 4.40e-36 | 141 |
| MS.gene53929.t1 | MTR_6g077680 | 47.500 | 240 | 48 | 5 | 6 | 168 | 172 | 410 | 1.07e-35 | 142 |
| MS.gene53929.t1 | MTR_6g078990 | 45.679 | 162 | 74 | 5 | 8 | 168 | 45 | 193 | 1.12e-24 | 103 |
| MS.gene53929.t1 | MTR_6g477680 | 76.389 | 72 | 17 | 0 | 97 | 168 | 257 | 328 | 2.47e-24 | 106 |
| MS.gene53929.t1 | MTR_5g067730 | 28.664 | 307 | 154 | 14 | 183 | 443 | 101 | 388 | 3.91e-22 | 100 |
| MS.gene53929.t1 | MTR_5g067730 | 29.057 | 265 | 127 | 12 | 224 | 443 | 5 | 253 | 4.26e-20 | 92.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53929.t1 | AT1G17850 | 66.485 | 367 | 109 | 5 | 182 | 536 | 75 | 439 | 7.84e-175 | 501 |
| MS.gene53929.t1 | AT1G17850 | 65.940 | 367 | 109 | 6 | 182 | 536 | 75 | 437 | 2.88e-171 | 492 |
| MS.gene53929.t1 | AT2G40760 | 29.355 | 310 | 145 | 12 | 184 | 443 | 98 | 383 | 5.99e-24 | 105 |
| MS.gene53929.t1 | AT1G09280 | 25.959 | 339 | 195 | 13 | 181 | 500 | 11 | 312 | 1.15e-17 | 86.7 |
Find 120 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAAACACAACACAGAATTT+TGG | 0.159102 | 3.2:-979499 | None:intergenic |
| TCTTTATCTACATTTGATAA+AGG | 0.246632 | 3.2:-985138 | None:intergenic |
| ACACCTCTCTTTCGTGGAAT+TGG | 0.268583 | 3.2:+982229 | MS.gene53929:CDS |
| TGACAGAGATTACATTATAT+TGG | 0.286731 | 3.2:+983644 | MS.gene53929:CDS |
| TTATACCGCAACTTCAATAC+TGG | 0.321060 | 3.2:-983199 | None:intergenic |
| AGTTAGAAACACCTCCCTTC+AGG | 0.326260 | 3.2:-986145 | None:intergenic |
| CTTTATCTACATTTGATAAA+GGG | 0.348482 | 3.2:-985137 | None:intergenic |
| TGCTTCAGGAGCACTTCATT+TGG | 0.363681 | 3.2:+984720 | MS.gene53929:CDS |
| TTCATTTGTCTGCAGTTTGA+AGG | 0.373195 | 3.2:+983507 | MS.gene53929:intron |
| TGTACTCGTCGAGCAGCTAA+TGG | 0.373574 | 3.2:-982104 | None:intergenic |
| GACCTAATGTAGACTGCTTC+AGG | 0.378595 | 3.2:+984706 | MS.gene53929:CDS |
| CTCTCTGGTAGCCTGGCCTC+TGG | 0.388186 | 3.2:-978934 | None:intergenic |
| AACTTATCATATCTAGAAAC+TGG | 0.389000 | 3.2:-986284 | None:intergenic |
| TTGCAATTTCTTGATTATCT+AGG | 0.408352 | 3.2:-979181 | None:intergenic |
| ATTATATTGGATGTGAGAAA+TGG | 0.413285 | 3.2:+983657 | MS.gene53929:CDS |
| CCTCTCTTTCGTGGAATTGG+AGG | 0.416632 | 3.2:+982232 | MS.gene53929:CDS |
| CTCTGCTCTCCATTTAAAAC+AGG | 0.420531 | 3.2:-986559 | None:intergenic |
| GAGGTGTTTCTAACTATATA+AGG | 0.429533 | 3.2:+986153 | MS.gene53929:CDS |
| GATATATTTCTACAGTATAG+TGG | 0.434778 | 3.2:+983073 | MS.gene53929:intron |
| CCACCTTCCGTAATGGCCTT+AGG | 0.436186 | 3.2:-986254 | None:intergenic |
| GTTTCTCAGAAGAATGTTGC+TGG | 0.438214 | 3.2:+979092 | MS.gene53929:intron |
| TAAATGGAGAGCAGAGATAC+AGG | 0.438585 | 3.2:+986566 | MS.gene53929:CDS |
| ACGCTATGGCATATGTCAAC+TGG | 0.445467 | 3.2:+983107 | MS.gene53929:CDS |
| AGATTCTTCCATTGATGTCT+AGG | 0.448549 | 3.2:-982919 | None:intergenic |
| CGCCAAACACCTCTCTTTCG+TGG | 0.455555 | 3.2:+982223 | MS.gene53929:CDS |
| TGACTTGCACTAGTGCTCCT+AGG | 0.458825 | 3.2:+986530 | MS.gene53929:CDS |
| GGGACATTGGACATTTCCGC+GGG | 0.461741 | 3.2:+984676 | MS.gene53929:CDS |
| TGGCTTACCTGTACTAAAGA+TGG | 0.464207 | 3.2:-983223 | None:intergenic |
| GTTAGAAACACCTCCCTTCA+GGG | 0.466131 | 3.2:-986144 | None:intergenic |
| TAAAAGGCTCTCTGGTAGCC+TGG | 0.468059 | 3.2:-978941 | None:intergenic |
| TGCCTTTAGCTCCTTCAGAA+TGG | 0.469052 | 3.2:+983574 | MS.gene53929:CDS |
| AGGATCGAGCAATGGAAGAT+TGG | 0.470747 | 3.2:-983538 | None:intergenic |
| TGACGATCCTAAGGCCATTA+CGG | 0.471938 | 3.2:+986247 | MS.gene53929:CDS |
| AAAGGCAATGCCCGCATTGA+AGG | 0.472830 | 3.2:-983558 | None:intergenic |
| GTTTGGCGACTAATTCTTGT+GGG | 0.473406 | 3.2:-982208 | None:intergenic |
| CAGGAGGAGGGAGAGACAAT+CGG | 0.473504 | 3.2:-986223 | None:intergenic |
| CTCCATTCTGAAGGAGCTAA+AGG | 0.478685 | 3.2:-983576 | None:intergenic |
| CTCCTGAAGCAGTCTACATT+AGG | 0.483249 | 3.2:-984708 | None:intergenic |
| TATCTAGGCAGTCAGATTCA+TGG | 0.487685 | 3.2:-979166 | None:intergenic |
| AGGAGGAGGGAGAGACAATC+GGG | 0.487790 | 3.2:-986222 | None:intergenic |
| CAGAATTTGTATACCCTGAA+GGG | 0.492896 | 3.2:+986131 | MS.gene53929:CDS |
| TTCCACGAAAGAGAGGTGTT+TGG | 0.493097 | 3.2:-982225 | None:intergenic |
| AATATAATGTAATCTCTGTC+AGG | 0.499827 | 3.2:-983642 | None:intergenic |
| CCTTTCCAGTATTGAAGTTG+CGG | 0.501981 | 3.2:+983194 | MS.gene53929:CDS |
| AAATTTCCTATCCATTCTGC+AGG | 0.502310 | 3.2:-986185 | None:intergenic |
| CCTAAGGCCATTACGGAAGG+TGG | 0.502666 | 3.2:+986254 | MS.gene53929:CDS |
| ACAAAATTCAAGTTCAAAGA+AGG | 0.503786 | 3.2:+982124 | MS.gene53929:CDS |
| TCAGGTTATGAGTGGGACAT+TGG | 0.504751 | 3.2:+984663 | MS.gene53929:intron |
| TCTAACTATATAAGGAATGA+AGG | 0.507826 | 3.2:+986161 | MS.gene53929:CDS |
| GAGAGCAGAGATACAGGAAA+TGG | 0.507827 | 3.2:+986572 | MS.gene53929:CDS |
| ACTGGATAAAAGAGGATAGC+AGG | 0.510047 | 3.2:+983125 | MS.gene53929:CDS |
| AGCACTGAACTTGATCAGTC+TGG | 0.513177 | 3.2:-979115 | None:intergenic |
| TGCAGAAAGAGGCGAGCAGA+AGG | 0.517112 | 3.2:+979256 | MS.gene53929:CDS |
| ATCTAGGCAGTCAGATTCAT+GGG | 0.518207 | 3.2:-979165 | None:intergenic |
| TTCTGTTTCCCTACCTCCAG+AGG | 0.519555 | 3.2:+979637 | MS.gene53929:CDS |
| TGAACAAGGGATTAATGCAC+AGG | 0.520270 | 3.2:+982949 | MS.gene53929:CDS |
| ATGGCCTTAGGATCGTCAAC+AGG | 0.523071 | 3.2:-986242 | None:intergenic |
| GAACCGAGCAACAATCTCTC+AGG | 0.525201 | 3.2:-979449 | None:intergenic |
| ATTTGGACTGTCTCAAGAAG+AGG | 0.527009 | 3.2:+984737 | MS.gene53929:CDS |
| TTAGGATCGTCAACAGGAGG+AGG | 0.527650 | 3.2:-986236 | None:intergenic |
| TCCGCTATTCAGGTTATGAG+TGG | 0.533918 | 3.2:+984655 | MS.gene53929:intron |
| ATTGCTCGATCCTTCAATGC+GGG | 0.535673 | 3.2:+983548 | MS.gene53929:CDS |
| TCAGAATTTGTATACCCTGA+AGG | 0.536005 | 3.2:+986130 | MS.gene53929:CDS |
| TTACTGATATTCCAGAGGCC+AGG | 0.538168 | 3.2:+978923 | MS.gene53929:CDS |
| AGTAGTCTCAATGCAGAAAG+AGG | 0.539374 | 3.2:+979245 | MS.gene53929:CDS |
| ATGGCATACCTCTGGAGGTA+GGG | 0.540599 | 3.2:-979645 | None:intergenic |
| TCCTCCTGTTGACGATCCTA+AGG | 0.541390 | 3.2:+986238 | MS.gene53929:CDS |
| CCGCTATTCAGGTTATGAGT+GGG | 0.544191 | 3.2:+984656 | MS.gene53929:intron |
| GGAATGAAGGTCCTGCAGAA+TGG | 0.545249 | 3.2:+986174 | MS.gene53929:CDS |
| AAATGTGTAAAGCATCTAAG+AGG | 0.548685 | 3.2:+986496 | MS.gene53929:CDS |
| TGTTTGGCGACTAATTCTTG+TGG | 0.550053 | 3.2:-982209 | None:intergenic |
| ATCAAGTAGAAATTCTTGCC+TGG | 0.551813 | 3.2:-986619 | None:intergenic |
| AGTCATCCCACCTTCCGTAA+TGG | 0.552531 | 3.2:-986261 | None:intergenic |
| GTAGAGATTACTCACACAAT+CGG | 0.552825 | 3.2:-979309 | None:intergenic |
| CGCATTGAAGGATCGAGCAA+TGG | 0.552955 | 3.2:-983546 | None:intergenic |
| AAATGCTACATATGTAATGC+GGG | 0.555589 | 3.2:+986311 | MS.gene53929:CDS |
| ATTTGTCTGCAGTTTGAAGG+TGG | 0.555821 | 3.2:+983510 | MS.gene53929:intron |
| TTTAAAACAGGTCTAAGCCT+AGG | 0.560426 | 3.2:-986547 | None:intergenic |
| TGGGACATTGGACATTTCCG+CGG | 0.563262 | 3.2:+984675 | MS.gene53929:CDS |
| GTATAAACCATCTTTAGTAC+AGG | 0.566195 | 3.2:+983216 | MS.gene53929:CDS |
| AGAATCTATTTGAATGAACA+AGG | 0.567800 | 3.2:+982935 | MS.gene53929:CDS |
| ATGCCATGTGGCAGGCAGCA+TGG | 0.574752 | 3.2:+986104 | MS.gene53929:intron |
| AACATATTGATGTATTGCAC+AGG | 0.579026 | 3.2:+985168 | MS.gene53929:CDS |
| TGGGCCATCGAAAGACGCTA+TGG | 0.580004 | 3.2:+983093 | MS.gene53929:CDS |
| ATATATTTCTACAGTATAGT+GGG | 0.580248 | 3.2:+983074 | MS.gene53929:intron |
| TGACAGGGCCTAGACATCAA+TGG | 0.580491 | 3.2:+982911 | MS.gene53929:intron |
| GAAGGTCCTGCAGAATGGAT+AGG | 0.580859 | 3.2:+986179 | MS.gene53929:CDS |
| ATCAAGAAATTGCAAGCACA+TGG | 0.582073 | 3.2:+979189 | MS.gene53929:CDS |
| GCACCTGAGAGATTGTTGCT+CGG | 0.583902 | 3.2:+979446 | MS.gene53929:CDS |
| TTCAAGTTCAAAGAAGGAGG+AGG | 0.586537 | 3.2:+982130 | MS.gene53929:CDS |
| TATCGTGACATGGATTTACC+AGG | 0.586675 | 3.2:+986601 | MS.gene53929:CDS |
| GATCCTAAGGCCATTACGGA+AGG | 0.594039 | 3.2:+986251 | MS.gene53929:CDS |
| ATATGTCAACTGGATAAAAG+AGG | 0.594088 | 3.2:+983117 | MS.gene53929:CDS |
| TATGCCATAGCGTCTTTCGA+TGG | 0.600373 | 3.2:-983097 | None:intergenic |
| CCTCCAATTCCACGAAAGAG+AGG | 0.603885 | 3.2:-982232 | None:intergenic |
| TTCGACTTTACACTCACAGA+AGG | 0.604181 | 3.2:+979539 | MS.gene53929:CDS |
| CCGCAACTTCAATACTGGAA+AGG | 0.605516 | 3.2:-983194 | None:intergenic |
| AGGCAGTCAGATTCATGGGA+TGG | 0.606368 | 3.2:-979161 | None:intergenic |
| ATATTGATGTATTGCACAGG+AGG | 0.611414 | 3.2:+985171 | MS.gene53929:CDS |
| GAGTCAATGAACTCAGACAT+CGG | 0.612715 | 3.2:+986333 | MS.gene53929:CDS |
| TTGATGGAGATATTTGAACC+AGG | 0.620449 | 3.2:-983159 | None:intergenic |
| TCCTCAAAATACCCTCTGCT+AGG | 0.621745 | 3.2:-979599 | None:intergenic |
| AAAATGCTACATATGTAATG+CGG | 0.630137 | 3.2:+986310 | MS.gene53929:CDS |
| CTAAGGCCATTACGGAAGGT+GGG | 0.630374 | 3.2:+986255 | MS.gene53929:CDS |
| ACAAGATGGCATACCTCTGG+AGG | 0.633022 | 3.2:-979650 | None:intergenic |
| AAATTCAAGTTCAAAGAAGG+AGG | 0.634628 | 3.2:+982127 | MS.gene53929:CDS |
| AACATGTCTAAACCTAGCAG+AGG | 0.638805 | 3.2:+979587 | MS.gene53929:CDS |
| GATGGCATACCTCTGGAGGT+AGG | 0.639419 | 3.2:-979646 | None:intergenic |
| TATTGATGTATTGCACAGGA+GGG | 0.641364 | 3.2:+985172 | MS.gene53929:CDS |
| GCCTTAGGATCGTCAACAGG+AGG | 0.644907 | 3.2:-986239 | None:intergenic |
| TAGGATCGTCAACAGGAGGA+GGG | 0.647577 | 3.2:-986235 | None:intergenic |
| GAATCTATTTGAATGAACAA+GGG | 0.655210 | 3.2:+982936 | MS.gene53929:CDS |
| ATGGCACGTCTATCGTGACA+TGG | 0.662813 | 3.2:+986591 | MS.gene53929:CDS |
| ACATGTCTAAACCTAGCAGA+GGG | 0.678399 | 3.2:+979588 | MS.gene53929:CDS |
| CATTGCTCGATCCTTCAATG+CGG | 0.678485 | 3.2:+983547 | MS.gene53929:CDS |
| CATTAGGTCGTTGAGCCCCG+CGG | 0.693023 | 3.2:-984692 | None:intergenic |
| AATTTGTATACCCTGAAGGG+AGG | 0.693215 | 3.2:+986134 | MS.gene53929:CDS |
| TAAGGTTACTGATATTCCAG+AGG | 0.698541 | 3.2:+978918 | MS.gene53929:CDS |
| ATGTGTATTCTACAATACTG+AGG | 0.745490 | 3.2:+985205 | MS.gene53929:CDS |
| GGACATTGGACATTTCCGCG+GGG | 0.748116 | 3.2:+984677 | MS.gene53929:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 978814 | 986657 | 978814 | ID=MS.gene53929 |
| chr3.2 | mRNA | 978814 | 986657 | 978814 | ID=MS.gene53929.t1;Parent=MS.gene53929 |
| chr3.2 | exon | 978814 | 978826 | 978814 | ID=MS.gene53929.t1.exon1;Parent=MS.gene53929.t1 |
| chr3.2 | CDS | 978814 | 978826 | 978814 | ID=cds.MS.gene53929.t1;Parent=MS.gene53929.t1 |
| chr3.2 | exon | 978917 | 978975 | 978917 | ID=MS.gene53929.t1.exon2;Parent=MS.gene53929.t1 |
| chr3.2 | CDS | 978917 | 978975 | 978917 | ID=cds.MS.gene53929.t1;Parent=MS.gene53929.t1 |
| chr3.2 | exon | 979101 | 979316 | 979101 | ID=MS.gene53929.t1.exon3;Parent=MS.gene53929.t1 |
| chr3.2 | CDS | 979101 | 979316 | 979101 | ID=cds.MS.gene53929.t1;Parent=MS.gene53929.t1 |
| chr3.2 | exon | 979443 | 979658 | 979443 | ID=MS.gene53929.t1.exon4;Parent=MS.gene53929.t1 |
| chr3.2 | CDS | 979443 | 979658 | 979443 | ID=cds.MS.gene53929.t1;Parent=MS.gene53929.t1 |
| chr3.2 | exon | 982104 | 982253 | 982104 | ID=MS.gene53929.t1.exon5;Parent=MS.gene53929.t1 |
| chr3.2 | CDS | 982104 | 982253 | 982104 | ID=cds.MS.gene53929.t1;Parent=MS.gene53929.t1 |
| chr3.2 | exon | 982917 | 982970 | 982917 | ID=MS.gene53929.t1.exon6;Parent=MS.gene53929.t1 |
| chr3.2 | CDS | 982917 | 982970 | 982917 | ID=cds.MS.gene53929.t1;Parent=MS.gene53929.t1 |
| chr3.2 | exon | 983088 | 983237 | 983088 | ID=MS.gene53929.t1.exon7;Parent=MS.gene53929.t1 |
| chr3.2 | CDS | 983088 | 983237 | 983088 | ID=cds.MS.gene53929.t1;Parent=MS.gene53929.t1 |
| chr3.2 | exon | 983522 | 983678 | 983522 | ID=MS.gene53929.t1.exon8;Parent=MS.gene53929.t1 |
| chr3.2 | CDS | 983522 | 983678 | 983522 | ID=cds.MS.gene53929.t1;Parent=MS.gene53929.t1 |
| chr3.2 | exon | 984667 | 984758 | 984667 | ID=MS.gene53929.t1.exon9;Parent=MS.gene53929.t1 |
| chr3.2 | CDS | 984667 | 984758 | 984667 | ID=cds.MS.gene53929.t1;Parent=MS.gene53929.t1 |
| chr3.2 | exon | 985123 | 985226 | 985123 | ID=MS.gene53929.t1.exon10;Parent=MS.gene53929.t1 |
| chr3.2 | CDS | 985123 | 985226 | 985123 | ID=cds.MS.gene53929.t1;Parent=MS.gene53929.t1 |
| chr3.2 | exon | 986118 | 986390 | 986118 | ID=MS.gene53929.t1.exon11;Parent=MS.gene53929.t1 |
| chr3.2 | CDS | 986118 | 986390 | 986118 | ID=cds.MS.gene53929.t1;Parent=MS.gene53929.t1 |
| chr3.2 | exon | 986486 | 986657 | 986486 | ID=MS.gene53929.t1.exon12;Parent=MS.gene53929.t1 |
| chr3.2 | CDS | 986486 | 986657 | 986486 | ID=cds.MS.gene53929.t1;Parent=MS.gene53929.t1 |
| Gene Sequence |
| Protein sequence |
