AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54269


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54269.t1 MTR_6g008560 71.579 190 53 1 1 189 1 190 3.59e-94 285
MS.gene54269.t1 MTR_5g062230 70.370 189 53 1 1 189 1 186 3.32e-91 277
MS.gene54269.t1 MTR_3g464190 69.841 189 56 1 1 188 1 189 2.91e-89 272
MS.gene54269.t1 MTR_6g039440 67.196 189 59 1 1 189 1 186 7.33e-87 266
MS.gene54269.t1 MTR_5g073200 69.444 180 54 1 12 190 4 183 3.39e-84 258
MS.gene54269.t1 MTR_5g073260 67.978 178 55 2 13 188 7 184 7.50e-80 248
MS.gene54269.t1 MTR_4g048460 61.326 181 70 0 8 188 13 193 1.20e-78 242
MS.gene54269.t1 MTR_4g048460 61.326 181 70 0 8 188 13 193 1.30e-77 242
MS.gene54269.t1 MTR_5g094620 60.638 188 55 1 1 188 1 169 1.47e-73 231
MS.gene54269.t1 MTR_4g081460 56.831 183 76 2 9 189 14 195 6.88e-65 209
MS.gene54269.t1 MTR_2g082010 45.251 179 97 1 13 190 23 201 2.46e-50 171
MS.gene54269.t1 MTR_2g081980 44.944 178 96 2 15 190 12 189 2.56e-48 165
MS.gene54269.t1 MTR_2g082060 40.933 193 111 2 1 190 3 195 7.24e-46 159
MS.gene54269.t1 MTR_8g007515 45.143 175 95 1 13 186 9 183 1.33e-44 155
MS.gene54269.t1 MTR_4g019550 44.828 174 94 2 15 186 19 192 8.74e-43 150
MS.gene54269.t1 MTR_4g045810 38.547 179 100 3 13 188 41 212 1.31e-34 128
MS.gene54269.t1 MTR_2g064425 45.882 170 84 4 17 185 53 215 1.54e-32 121
MS.gene54269.t1 MTR_2g064425 45.882 170 84 4 17 185 53 215 5.42e-32 121
MS.gene54269.t1 MTR_8g057020 38.636 176 102 3 10 185 48 217 1.36e-30 117
MS.gene54269.t1 MTR_2g065450 35.795 176 105 3 10 185 51 218 3.58e-26 104
MS.gene54269.t1 MTR_2g089120 38.028 142 74 3 49 187 67 197 2.52e-25 102
MS.gene54269.t1 MTR_2g089130 36.054 147 80 3 44 187 62 197 6.98e-25 101
MS.gene54269.t1 MTR_2g082050 37.838 148 69 2 43 190 1 125 7.87e-25 100
MS.gene54269.t1 MTR_2g064425 40.588 170 78 5 17 185 53 200 1.24e-22 94.0
MS.gene54269.t1 MTR_2g064425 40.588 170 78 5 17 185 53 200 2.17e-22 94.0
MS.gene54269.t1 MTR_4g092010 35.766 137 86 2 55 189 61 197 2.90e-21 90.9
MS.gene54269.t1 MTR_7g010710 30.645 186 123 4 1 185 1 181 1.33e-20 89.0
MS.gene54269.t1 MTR_4g092020 36.567 134 83 1 56 189 72 203 1.47e-20 88.6
MS.gene54269.t1 MTR_2g010960 35.461 141 85 2 47 186 9 144 2.82e-20 87.8
MS.gene54269.t1 MTR_7g094970 34.959 123 74 2 68 184 206 328 1.20e-16 77.4
MS.gene54269.t1 MTR_7g011663 33.871 124 76 2 68 185 344 467 3.98e-16 75.9
MS.gene54269.t1 MTR_3g465090 38.211 123 74 2 54 175 13 134 1.88e-15 73.9
MS.gene54269.t1 MTR_6g064980 38.136 118 71 2 54 170 13 129 3.31e-15 73.2
MS.gene54269.t1 MTR_3g063170 35.484 124 78 2 54 176 13 135 3.51e-15 73.2
MS.gene54269.t1 MTR_7g011770 31.707 123 78 2 68 184 240 362 1.73e-14 71.2
MS.gene54269.t1 MTR_3g063160 35.484 124 78 2 54 176 13 135 1.74e-14 70.1
MS.gene54269.t1 MTR_3g058160 36.220 127 75 4 64 185 267 392 3.73e-14 70.5
MS.gene54269.t1 MTR_3g052120 34.848 132 83 3 54 184 13 142 3.79e-14 70.1
MS.gene54269.t1 MTR_3g058160 36.441 118 72 3 70 185 256 372 3.84e-14 70.5
MS.gene54269.t1 MTR_3g058160 36.441 118 72 3 70 185 276 392 3.86e-14 70.5
MS.gene54269.t1 MTR_3g058160 36.441 118 72 3 70 185 269 385 3.89e-14 70.1
MS.gene54269.t1 MTR_3g058160 36.441 118 72 3 70 185 65 181 4.77e-14 70.1
MS.gene54269.t1 MTR_2g064295 34.746 118 74 3 70 185 256 372 5.96e-14 69.7
MS.gene54269.t1 MTR_3g063140 34.677 124 79 2 54 176 13 135 3.22e-13 67.4
MS.gene54269.t1 MTR_5g030050 35.965 114 63 2 73 184 3 108 4.31e-13 66.6
MS.gene54269.t1 MTR_3g063120 35.135 111 70 1 68 176 1 111 1.16e-12 65.9
MS.gene54269.t1 MTR_6g008560 82.353 34 6 0 156 189 1 34 4.25e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54269.t1 AT5G23960 39.011 182 101 5 13 189 3 179 8.06e-35 129
MS.gene54269.t1 AT5G23960 39.011 182 101 5 13 189 3 179 8.28e-35 129
MS.gene54269.t1 AT1G70080 38.889 180 106 4 8 185 57 234 3.52e-34 127
MS.gene54269.t1 AT3G14540 37.500 168 102 2 22 189 65 229 2.36e-32 121
MS.gene54269.t1 AT3G14540 37.500 168 102 2 22 189 65 229 5.23e-32 121
MS.gene54269.t1 AT1G33750 40.606 165 95 2 24 188 73 234 8.19e-32 120
MS.gene54269.t1 AT3G29410 37.853 177 98 3 17 188 64 233 9.40e-32 120
MS.gene54269.t1 AT3G14490 38.068 176 103 4 17 190 60 231 3.94e-31 119
MS.gene54269.t1 AT5G44630 39.106 179 96 5 18 189 14 186 5.77e-31 118
MS.gene54269.t1 AT1G31950 35.227 176 96 4 22 189 70 235 7.64e-31 117
MS.gene54269.t1 AT1G31950 35.227 176 96 4 22 189 70 235 1.19e-30 117
MS.gene54269.t1 AT1G31950 36.686 169 102 3 22 189 70 234 1.52e-30 117
MS.gene54269.t1 AT3G14520 37.349 166 99 3 22 186 68 229 2.02e-30 117
MS.gene54269.t1 AT4G15870 41.071 168 91 5 25 188 75 238 3.12e-29 113
MS.gene54269.t1 AT4G13300 38.554 166 95 4 25 187 20 181 4.91e-28 110
MS.gene54269.t1 AT4G20210 35.955 178 105 4 17 190 58 230 7.19e-28 109
MS.gene54269.t1 AT3G32030 35.450 189 111 5 2 188 54 233 7.84e-28 109
MS.gene54269.t1 AT4G20200 40.000 145 79 3 48 188 92 232 7.99e-28 109
MS.gene54269.t1 AT3G29110 37.500 176 105 4 17 190 55 227 1.24e-27 108
MS.gene54269.t1 AT4G20230 38.621 145 81 3 48 188 92 232 1.34e-27 108
MS.gene54269.t1 AT4G20230 38.621 145 81 3 48 188 104 244 1.35e-27 108
MS.gene54269.t1 AT1G48800 37.079 178 103 4 17 190 63 235 3.87e-27 107
MS.gene54269.t1 AT1G66020 35.057 174 108 3 17 188 56 226 2.91e-26 104
MS.gene54269.t1 AT1G66020 35.057 174 108 3 17 188 56 226 4.91e-26 104
MS.gene54269.t1 AT2G23230 35.754 179 104 5 16 188 55 228 1.61e-25 103
MS.gene54269.t1 AT2G23230 35.754 179 104 5 16 188 55 228 1.78e-25 102
MS.gene54269.t1 AT3G29410 40.000 130 76 1 59 188 1 128 7.40e-25 100
MS.gene54269.t1 AT3G29190 35.862 145 89 2 48 190 89 231 1.02e-24 100
MS.gene54269.t1 AT2G24210 35.758 165 99 3 10 174 39 196 6.57e-24 98.6
MS.gene54269.t1 AT4G13280 37.195 164 96 4 27 187 22 181 2.69e-23 96.7
MS.gene54269.t1 AT4G13280 37.195 164 96 4 27 187 22 181 2.84e-23 96.7
MS.gene54269.t1 AT1G61680 37.956 137 78 2 54 189 77 207 4.69e-23 95.9
MS.gene54269.t1 AT4G16740 32.240 183 109 6 12 188 21 194 1.05e-22 94.0
MS.gene54269.t1 AT4G16740 32.597 181 107 6 14 188 23 194 1.06e-22 94.0
MS.gene54269.t1 AT4G16740 32.597 181 107 6 14 188 23 194 1.06e-22 94.0
MS.gene54269.t1 AT4G16730 37.107 159 94 4 28 184 2 156 1.99e-22 94.0
MS.gene54269.t1 AT4G16740 32.778 180 106 6 12 185 21 191 2.40e-22 94.0
MS.gene54269.t1 AT3G25820 33.529 170 98 5 15 177 45 206 2.92e-20 87.4
MS.gene54269.t1 AT3G25820 33.529 170 98 5 15 177 45 206 2.92e-20 87.4
MS.gene54269.t1 AT3G25830 33.529 170 98 5 15 177 45 206 3.57e-20 87.8
MS.gene54269.t1 AT3G25820 33.529 170 98 5 15 177 45 206 3.57e-20 87.8
MS.gene54269.t1 AT3G25820 32.065 184 109 6 15 190 45 220 3.89e-20 87.4
MS.gene54269.t1 AT1G61680 36.496 137 73 3 54 189 77 200 9.29e-20 86.7
MS.gene54269.t1 AT4G16730 41.593 113 62 2 73 184 1 110 3.14e-19 84.7
MS.gene54269.t1 AT1G48820 34.104 173 87 5 17 189 63 208 7.85e-19 84.0
MS.gene54269.t1 AT4G02780 35.484 124 72 3 68 184 329 451 2.45e-16 76.6
MS.gene54269.t1 AT3G25810 34.815 135 75 5 63 190 93 221 9.85e-16 74.7

Find 0 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATTATAAACTAATATTAA+TGG + 43198:30671-30690 MS.gene54269:intron 10.0%
!!! CATTTTTTATGTTTTTAATT+TGG + 43198:30304-30323 MS.gene54269:intron 10.0%
!! AATTGATAAACTAAAAAAGA+AGG + 43198:30018-30037 MS.gene54269:CDS 15.0%
!! ATGAGTAAATAAATAGTATA+AGG - 43198:29853-29872 MS.gene54269:intergenic 15.0%
!! TAATATGAATGTCTAAAAAA+TGG + 43198:30436-30455 MS.gene54269:intron 15.0%
!!! ATTGTTGTTTAATATTATTG+TGG + 43198:30833-30852 MS.gene54269:intron 15.0%
!! AAGACACTTAATTATTGTTA+TGG - 43198:29926-29945 MS.gene54269:intergenic 20.0%
!! AATATTAATGGTATACATGT+AGG + 43198:30683-30702 MS.gene54269:intron 20.0%
!! GAGAATAATTAAAAGAAATG+AGG - 43198:30612-30631 MS.gene54269:intergenic 20.0%
!!! TATATGTTTTGAATACATAC+AGG + 43198:29970-29989 MS.gene54269:intron 20.0%
! TACAAGAGTTATGTTCAAAA+TGG + 43198:30166-30185 MS.gene54269:CDS 25.0%
! TTGAGTAAAACAAATTAGAG+TGG + 43198:30335-30354 MS.gene54269:intron 25.0%
!! AATAAGTTGAGATATATACC+AGG - 43198:30271-30290 MS.gene54269:intergenic 25.0%
!! AGACGAAATTAATCAAGTTT+TGG + 43198:30135-30154 MS.gene54269:CDS 25.0%
!! GTAATATTTGACTTTGTCAT+GGG + 43198:30407-30426 MS.gene54269:intron 25.0%
AAAGATTTACAAGCTAACAG+TGG - 43198:30466-30485 MS.gene54268:intergenic 30.0%
AAATGTGAGTAACTTGAATC+TGG - 43198:29829-29848 MS.gene54268:intergenic 30.0%
AATGTGAGTAACTTGAATCT+GGG - 43198:29828-29847 MS.gene54269:intergenic 30.0%
ATGTGAACATTACACAGATA+AGG - 43198:30589-30608 MS.gene54269:intergenic 30.0%
CATCAATCCTAATGAGAAAT+CGG + 43198:29622-29641 MS.gene54269:CDS 30.0%
GGTTCATGGTGAAAAAATAT+TGG + 43198:30976-30995 MS.gene54269:CDS 30.0%
TGGTGAAAAAATATTGGAAG+AGG + 43198:30982-31001 MS.gene54269:CDS 30.0%
! ATTGACTCAATATGTCGTTT+GGG + 43198:30094-30113 MS.gene54269:CDS 30.0%
! CGTAATATTTGACTTTGTCA+TGG + 43198:30406-30425 MS.gene54269:intron 30.0%
! TTTTGCTAAATGCAGTTCAT+AGG + 43198:29895-29914 MS.gene54269:intron 30.0%
!! AATAGTGATTGAAGAGTACT+AGG + 43198:29765-29784 MS.gene54269:intron 30.0%
!! TGTTTCTGAAGTACGAAAAA+GGG - 43198:30725-30744 MS.gene54269:intergenic 30.0%
!!! AATCAAGTTGACTTTTTCCA+AGG - 43198:30077-30096 MS.gene54269:intergenic 30.0%
!!! ATCAAGTTGACTTTTTCCAA+GGG - 43198:30076-30095 MS.gene54269:intergenic 30.0%
AGATGTGTTCAACCAATTCA+AGG + 43198:30874-30893 MS.gene54269:intron 35.0%
AGGAAACTTCAGTGAAACAT+TGG + 43198:30904-30923 MS.gene54269:CDS 35.0%
GAATCTGGGCTAATATTAAG+TGG - 43198:29814-29833 MS.gene54269:intergenic 35.0%
TCCTTATGCTTCAGAATCTA+TGG + 43198:29700-29719 MS.gene54269:CDS 35.0%
! AAAAATATTGGAAGAGGCGT+TGG + 43198:30988-31007 MS.gene54269:CDS 35.0%
! GATTGACTCAATATGTCGTT+TGG + 43198:30093-30112 MS.gene54269:CDS 35.0%
! TGCAAAACCGATTTCTCATT+AGG - 43198:29632-29651 MS.gene54269:intergenic 35.0%
!! ACCATAGATTCTGAAGCATA+AGG - 43198:29704-29723 MS.gene54269:intergenic 35.0%
!! ATTCTCTTGCTGTGCTATTT+AGG + 43198:30215-30234 MS.gene54269:CDS 35.0%
!! GTGTTTCTGAAGTACGAAAA+AGG - 43198:30726-30745 MS.gene54269:intergenic 35.0%
!! TTCTCTTGCTGTGCTATTTA+GGG + 43198:30216-30235 MS.gene54269:CDS 35.0%
AAAAAATCTCCCCAAACGCT+AGG - 43198:29677-29696 MS.gene54268:intergenic 40.0%
AAAAATCTCCCCAAACGCTA+GGG - 43198:29676-29695 MS.gene54268:intergenic 40.0%
AACATTGGTCAGAGATGTTG+AGG + 43198:30919-30938 MS.gene54269:CDS 40.0%
ACATTGGTCAGAGATGTTGA+GGG + 43198:30920-30939 MS.gene54269:CDS 40.0%
ACCATGAACCATCATATGTG+AGG - 43198:30966-30985 MS.gene54269:intergenic 40.0%
ATAGCACAGCAAGAGAATGA+AGG - 43198:30213-30232 MS.gene54269:intergenic 40.0%
CTCAAAAACAGAGAAACCCT+TGG + 43198:30057-30076 MS.gene54269:CDS 40.0%
GGAACCTGATATGAATACTG+CGG + 43198:29991-30010 MS.gene54269:intron 40.0%
GTATGAAGCCTCACATATGA+TGG + 43198:30955-30974 MS.gene54269:CDS 40.0%
!! AGGAGTGTCTTTCGTTTGAT+CGG + 43198:30546-30565 MS.gene54269:intron 40.0%
AACCAATTCAAGGACGAGCA+AGG + 43198:30884-30903 MS.gene54269:CDS 45.0%
AGATTTCCACCCTAGCGTTT+GGG + 43198:29664-29683 MS.gene54269:CDS 45.0%
CATGGACTTTACGTTTCACC+TGG + 43198:30250-30269 MS.gene54269:CDS 45.0%
GCCTCACATATGATGGTTCA+TGG + 43198:30962-30981 MS.gene54269:CDS 45.0%
TGTGCCGCAGTATTCATATC+AGG - 43198:29998-30017 MS.gene54269:intergenic 45.0%
TTCCTTGCTCGTCCTTGAAT+TGG - 43198:30889-30908 MS.gene54269:intergenic 45.0%
!! CTGTGCTATTTAGGGTGTTG+AGG + 43198:30224-30243 MS.gene54269:CDS 45.0%
!! TTTAGGGTGTTGAGGCAACA+TGG + 43198:30232-30251 MS.gene54269:CDS 45.0%
!!! ATTTTTTTTTTACATAAAAT+AGG + 43198:30526-30545 MS.gene54269:intron 5.0%
CAGATTTCCACCCTAGCGTT+TGG + 43198:29663-29682 MS.gene54269:CDS 50.0%
GATTTCCACCCTAGCGTTTG+GGG + 43198:29665-29684 MS.gene54269:CDS 50.0%
TTCTGGTCACCTTGAGTCCA+TGG + 43198:31018-31037 MS.gene54269:CDS 50.0%
!!! GAGGCGTTGGCTTTTACTTC+TGG + 43198:31001-31020 MS.gene54269:CDS 50.0%
AATCGGTGGCCATGGACTCA+AGG - 43198:31030-31049 MS.gene54269:intergenic 55.0%
AATCTCCCCAAACGCTAGGG+TGG - 43198:29673-29692 MS.gene54269:intergenic 55.0%


Chromosome Type Strat End Strand Name
43198 gene 29605 31051 29605 ID=MS.gene54269
43198 mRNA 29605 31051 29605 ID=MS.gene54269.t1;Parent=MS.gene54269
43198 exon 29605 29721 29605 ID=MS.gene54269.t1.exon1;Parent=MS.gene54269.t1
43198 CDS 29605 29721 29605 ID=cds.MS.gene54269.t1;Parent=MS.gene54269.t1
43198 exon 29992 30271 29992 ID=MS.gene54269.t1.exon2;Parent=MS.gene54269.t1
43198 CDS 29992 30271 29992 ID=cds.MS.gene54269.t1;Parent=MS.gene54269.t1
43198 exon 30876 31051 30876 ID=MS.gene54269.t1.exon3;Parent=MS.gene54269.t1
43198 CDS 30876 31051 30876 ID=cds.MS.gene54269.t1;Parent=MS.gene54269.t1
Gene Sequence

>MS.gene54269

ATGTCTTCAGCAGATTACATCAATCCTAATGAGAAATCGGTTTTGCAAAGAAATGTTGCAGATTTCCACCCTAGCGTTTGGGGAGATTTTTTCATTCCTTATGCTTCAGAATCTATGGTACGTAATTCTTTAAATCAACATCAAGTTTATATATATTATGAATAGTGATTGAAGAGTACTAGGAACATTATCACGTATGCAATAAGCCACTTAATATTAGCCCAGATTCAAGTTACTCACATTTACCTTATACTATTTATTTACTCATGAATGACAATAATGTAGACTCATTTTGCTAAATGCAGTTCATAGGTTGATCCATAACAATAATTAAGTGTCTTTTATCAATTTATTCTAAAATCATATATATGTTTTGAATACATACAGGAACCTGATATGAATACTGCGGCACAAATTGATAAACTAAAAAAGAAGGTTAGACAAATGCTTGTCTCAAAAACAGAGAAACCCTTGGAAAAAGTCAACTTGATTGACTCAATATGTCGTTTGGGTGTGAGCTATCATTTTGAAGACGAAATTAATCAAGTTTTGGAACATATTTACAAGAGTTATGTTCAAAATGGAGAAATAACTCTCAAAGACAACCTTCATTCTCTTGCTGTGCTATTTAGGGTGTTGAGGCAACATGGACTTTACGTTTCACCTGGTATATATCTCAACTTATTCACATTAATGATTCATTTTTTATGTTTTTAATTTGGATCGCAAATTGAGTAAAACAAATTAGAGTGGTAAACATTAAAAAAAATCTAATTGTAAAATCAATACTTAAATTAACAACGTAATATTTGACTTTGTCATGGGTGTGTCTAATATGAATGTCTAAAAAATGGCATACCACTGTTAGCTTGTAAATCTTTTAGTAAAAAGAGAATTTTATAATAATAAAAGCTAGAAAATATTTTTTTTTTACATAAAATAGGAGTGTCTTTCGTTTGATCGGATACATATTAGACAACTCCTTATCTGTGTAATGTTCACATCCTCATTTCTTTTAATTATTCTCTTTTAATTTTTTTTTTATATCACAGAAATAGACACTTAATGATTATAAACTAATATTAATGGTATACATGTAGGATCTTATTAAGTAAGACCCTTTTTCGTACTTCAGAAACACGCTATACATTATCTAGATTGCATAATGAATTTTTCAATCTTGCTTTAGCTTCTTATTTATATATATTTATAAATTAATATGTCTCTAATTGTTGTTTAATATTATTGTGGTTGTCTAATAAATATACTAGATGTGTTCAACCAATTCAAGGACGAGCAAGGAAACTTCAGTGAAACATTGGTCAGAGATGTTGAGGGCATGTTAAACTTGTATGAAGCCTCACATATGATGGTTCATGGTGAAAAAATATTGGAAGAGGCGTTGGCTTTTACTTCTGGTCACCTTGAGTCCATGGCCACCGATTGA

Protein sequence

>MS.gene54269.t1

MSSADYINPNEKSVLQRNVADFHPSVWGDFFIPYASESMEPDMNTAAQIDKLKKKVRQMLVSKTEKPLEKVNLIDSICRLGVSYHFEDEINQVLEHIYKSYVQNGEITLKDNLHSLAVLFRVLRQHGLYVSPDVFNQFKDEQGNFSETLVRDVEGMLNLYEASHMMVHGEKILEEALAFTSGHLESMATD