Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54320.t1 | XP_003593755.1 | 53.8 | 314 | 56 | 4 | 1 | 227 | 1 | 312 | 1.50E-77 | 299.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54320.t1 | G7ILH6 | 53.8 | 314 | 56 | 4 | 1 | 227 | 1 | 312 | 1.1e-77 | 299.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055056 | MS.gene54320 | 0.832756 | 7.88E-56 | -1.69E-46 |
| MS.gene058515 | MS.gene54320 | 0.814565 | 1.44E-51 | -1.69E-46 |
| MS.gene05925 | MS.gene54320 | 0.815344 | 9.70E-52 | -1.69E-46 |
| MS.gene059276 | MS.gene54320 | 0.821393 | 4.14E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54320.t1 | MTR_2g015760 | 45.223 | 314 | 83 | 8 | 1 | 227 | 1 | 312 | 2.46e-68 | 213 |
| MS.gene54320.t1 | MTR_2g015750 | 89.130 | 46 | 5 | 0 | 1 | 46 | 1 | 46 | 3.41e-22 | 87.0 |
| MS.gene54320.t1 | MTR_2g015750 | 75.556 | 45 | 11 | 0 | 183 | 227 | 42 | 86 | 4.52e-18 | 76.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 47 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGATGATCATCTCCTTAA+AGG | 0.136143 | 2.3:-69572609 | MS.gene54320:CDS |
| GCACGATCCATACTCCTTTA+AGG | 0.163291 | 2.3:+69572595 | None:intergenic |
| ATGGAGCGAGTGTAGTTTAT+TGG | 0.249967 | 2.3:-69572838 | MS.gene54320:CDS |
| ACTCTATGATGCTCAAAATT+TGG | 0.258194 | 2.3:-69572664 | MS.gene54320:CDS |
| CAAGTTGACCCAGCCAGTTC+TGG | 0.303744 | 2.3:+69573228 | None:intergenic |
| ATCATCTCCTTAAAGGAGTA+TGG | 0.340645 | 2.3:-69572602 | MS.gene54320:CDS |
| TCTTAGTGTTTATGATATCT+TGG | 0.361386 | 2.3:+69572573 | None:intergenic |
| TGCTGTCAAATATTGCAAAT+AGG | 0.363050 | 2.3:-69573404 | MS.gene54320:CDS |
| GATTGGCGCTCTAAATTTAG+TGG | 0.386788 | 2.3:-69573339 | MS.gene54320:CDS |
| ACTACACTCGCTCCATTGTT+TGG | 0.401720 | 2.3:+69572845 | None:intergenic |
| TTCATCATGTGTGCAAAGAT+TGG | 0.403215 | 2.3:-69573356 | MS.gene54320:CDS |
| TATAACTACAAGTTTATTGC+TGG | 0.411591 | 2.3:+69572884 | None:intergenic |
| TGAAAGGAAAACTAGGACTT+GGG | 0.413009 | 2.3:-69572716 | MS.gene54320:CDS |
| CACTATCGAAATTCCAGAAC+TGG | 0.428141 | 2.3:-69573241 | MS.gene54320:CDS |
| CTGAAAGGAAAACTAGGACT+TGG | 0.430022 | 2.3:-69572717 | MS.gene54320:CDS |
| TGGTGTGGGGTGATTACATT+TGG | 0.434879 | 2.3:-69572644 | MS.gene54320:CDS |
| GATCCATGGTTTCTGTTGTA+TGG | 0.439332 | 2.3:-69573312 | MS.gene54320:CDS |
| GTCAAATATTGCAAATAGGC+TGG | 0.448250 | 2.3:-69573400 | MS.gene54320:CDS |
| AGGTGTGCATTCTTTATTGA+AGG | 0.450535 | 2.3:-69572921 | MS.gene54320:CDS |
| GCCTTGCATCCTACGACGGA+TGG | 0.482267 | 2.3:-69573206 | MS.gene54320:CDS |
| TTTATCATCCTTGTTTAGCA+TGG | 0.488027 | 2.3:+69573269 | None:intergenic |
| ATCGAAATTCCAGAACTGGC+TGG | 0.492699 | 2.3:-69573237 | MS.gene54320:CDS |
| ATATTTGACAGCAGATCATA+AGG | 0.498174 | 2.3:+69573414 | None:intergenic |
| AGTATTGAAATGTAGATTGA+GGG | 0.511372 | 2.3:-69572787 | MS.gene54320:CDS |
| GGTGTGCATTCTTTATTGAA+GGG | 0.534190 | 2.3:-69572920 | MS.gene54320:CDS |
| CAGTATTGAAATGTAGATTG+AGG | 0.535602 | 2.3:-69572788 | MS.gene54320:CDS |
| ATGTTGCAATAGATACTTGT+GGG | 0.537656 | 2.3:-69572689 | MS.gene54320:CDS |
| AATTTAGTGGATGTGATCCA+TGG | 0.540102 | 2.3:-69573326 | MS.gene54320:CDS |
| AAGGATCTGAAAGGAAAACT+AGG | 0.543003 | 2.3:-69572723 | MS.gene54320:CDS |
| TCGAAATTCCAGAACTGGCT+GGG | 0.550468 | 2.3:-69573236 | MS.gene54320:CDS |
| GTGTGCATTCTTTATTGAAG+GGG | 0.552789 | 2.3:-69572919 | MS.gene54320:CDS |
| GCCATCCGTCGTAGGATGCA+AGG | 0.563825 | 2.3:+69573205 | None:intergenic |
| TGATGCTCAAAATTTGGTGT+GGG | 0.569401 | 2.3:-69572658 | MS.gene54320:CDS |
| TTTCTGTTGTATGGTGAAGA+AGG | 0.575330 | 2.3:-69573303 | MS.gene54320:CDS |
| AAAGGGAAAGCATCGATCAA+AGG | 0.578404 | 2.3:-69572742 | MS.gene54320:CDS |
| ATGATGCTCAAAATTTGGTG+TGG | 0.583531 | 2.3:-69572659 | MS.gene54320:CDS |
| GAGAAGCAGCCATCCGTCGT+AGG | 0.605702 | 2.3:+69573197 | None:intergenic |
| TCGTTGTTCCATGCTAAACA+AGG | 0.611298 | 2.3:-69573277 | MS.gene54320:CDS |
| TCACCATACAACAGAAACCA+TGG | 0.617900 | 2.3:+69573309 | None:intergenic |
| CATCGATCAAAGGATCTGAA+AGG | 0.623852 | 2.3:-69572732 | MS.gene54320:CDS |
| AAAAGAAAAGCAAAGGAGAG+TGG | 0.624016 | 2.3:-69573446 | MS.gene54320:CDS |
| ACTTGCCTTGCATCCTACGA+CGG | 0.635214 | 2.3:-69573210 | MS.gene54320:CDS |
| GAAAGGAAAACTAGGACTTG+GGG | 0.666358 | 2.3:-69572715 | MS.gene54320:CDS |
| AATGTTGCAATAGATACTTG+TGG | 0.675405 | 2.3:-69572690 | MS.gene54320:CDS |
| ACGTTGCTAATACCAAACAA+TGG | 0.689270 | 2.3:-69572857 | MS.gene54320:CDS |
| GATGCTCAAAATTTGGTGTG+GGG | 0.729523 | 2.3:-69572657 | MS.gene54320:CDS |
| TTGTTTAGCATGGAACAACG+AGG | 0.763244 | 2.3:+69573279 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAATTTGTTTCTCTTTAAA+AGG | - | chr2.3:69573108-69573127 | MS.gene54320:intron | 15.0% |
| !! | AGAGAAACAAATTATATTCA+TGG | + | chr2.3:69573103-69573122 | None:intergenic | 20.0% |
| ! | ATATGCAAAAAGAAAAGCAA+AGG | - | chr2.3:69572596-69572615 | MS.gene54320:CDS | 25.0% |
| ! | ATTGTCAAAGAAAGCAAAAA+AGG | - | chr2.3:69573289-69573308 | MS.gene54320:CDS | 25.0% |
| ! | GAAACAAATTATATTCATGG+TGG | + | chr2.3:69573100-69573119 | None:intergenic | 25.0% |
| ! | TATAACTACAAGTTTATTGC+TGG | + | chr2.3:69573168-69573187 | None:intergenic | 25.0% |
| ! | TATATTCATGGTGGTAAAAT+AGG | + | chr2.3:69573091-69573110 | None:intergenic | 25.0% |
| ! | TTGTCAAAGAAAGCAAAAAA+GGG | - | chr2.3:69573290-69573309 | MS.gene54320:CDS | 25.0% |
| !! | AGTATTGAAATGTAGATTGA+GGG | - | chr2.3:69573262-69573281 | MS.gene54320:CDS | 25.0% |
| !! | CAGTTCTAATTTATATTGAG+TGG | + | chr2.3:69573030-69573049 | None:intergenic | 25.0% |
| !!! | ATGTATTTTTTCTCTAGAGA+AGG | + | chr2.3:69572910-69572929 | None:intergenic | 25.0% |
| !!! | TGTATTTTTTCTCTAGAGAA+GGG | + | chr2.3:69572909-69572928 | None:intergenic | 25.0% |
| !!! | TTTGCTTTTCTTTTTGCATA+TGG | + | chr2.3:69572597-69572616 | None:intergenic | 25.0% |
| AATGTTGCAATAGATACTTG+TGG | - | chr2.3:69573359-69573378 | MS.gene54320:CDS | 30.0% | |
| ACTCTATGATGCTCAAAATT+TGG | - | chr2.3:69573385-69573404 | MS.gene54320:CDS | 30.0% | |
| ATATTTGACAGCAGATCATA+AGG | + | chr2.3:69572638-69572657 | None:intergenic | 30.0% | |
| ATGTTGCAATAGATACTTGT+GGG | - | chr2.3:69573360-69573379 | MS.gene54320:CDS | 30.0% | |
| TGCTGTCAAATATTGCAAAT+AGG | - | chr2.3:69572645-69572664 | MS.gene54320:CDS | 30.0% | |
| ! | CAGTATTGAAATGTAGATTG+AGG | - | chr2.3:69573261-69573280 | MS.gene54320:CDS | 30.0% |
| ! | TTTATCATCCTTGTTTAGCA+TGG | + | chr2.3:69572783-69572802 | None:intergenic | 30.0% |
| ! | TTTGATGATCATCTCCTTAA+AGG | - | chr2.3:69573440-69573459 | MS.gene54320:CDS | 30.0% |
| !!! | GTATTTTTTCTCTAGAGAAG+GGG | + | chr2.3:69572908-69572927 | None:intergenic | 30.0% |
| AAAAGAAAAGCAAAGGAGAG+TGG | - | chr2.3:69572603-69572622 | MS.gene54320:CDS | 35.0% | |
| AAGGATCTGAAAGGAAAACT+AGG | - | chr2.3:69573326-69573345 | MS.gene54320:CDS | 35.0% | |
| ACGTTGCTAATACCAAACAA+TGG | - | chr2.3:69573192-69573211 | MS.gene54320:CDS | 35.0% | |
| AGGTGTGCATTCTTTATTGA+AGG | - | chr2.3:69573128-69573147 | MS.gene54320:intron | 35.0% | |
| ATCATCTCCTTAAAGGAGTA+TGG | - | chr2.3:69573447-69573466 | MS.gene54320:CDS | 35.0% | |
| GATTCTGTGATAGGACAATT+TGG | + | chr2.3:69572936-69572955 | None:intergenic | 35.0% | |
| GGTGTGCATTCTTTATTGAA+GGG | - | chr2.3:69573129-69573148 | MS.gene54320:intron | 35.0% | |
| GTCAAATATTGCAAATAGGC+TGG | - | chr2.3:69572649-69572668 | MS.gene54320:CDS | 35.0% | |
| GTGTGCATTCTTTATTGAAG+GGG | - | chr2.3:69573130-69573149 | MS.gene54320:intron | 35.0% | |
| TGAAAGGAAAACTAGGACTT+GGG | - | chr2.3:69573333-69573352 | MS.gene54320:CDS | 35.0% | |
| TTCATCATGTGTGCAAAGAT+TGG | - | chr2.3:69572693-69572712 | MS.gene54320:CDS | 35.0% | |
| TTTCTGTTGTATGGTGAAGA+AGG | - | chr2.3:69572746-69572765 | MS.gene54320:CDS | 35.0% | |
| ! | AATTTAGTGGATGTGATCCA+TGG | - | chr2.3:69572723-69572742 | MS.gene54320:CDS | 35.0% |
| ! | ATGATGCTCAAAATTTGGTG+TGG | - | chr2.3:69573390-69573409 | MS.gene54320:CDS | 35.0% |
| !! | TGATGCTCAAAATTTGGTGT+GGG | - | chr2.3:69573391-69573410 | MS.gene54320:CDS | 35.0% |
| ATGGAGCGAGTGTAGTTTAT+TGG | - | chr2.3:69573211-69573230 | MS.gene54320:CDS | 40.0% | |
| CACTATCGAAATTCCAGAAC+TGG | - | chr2.3:69572808-69572827 | MS.gene54320:CDS | 40.0% | |
| CAGTCATTCGATTCTGTGAT+AGG | + | chr2.3:69572945-69572964 | None:intergenic | 40.0% | |
| CATCGATCAAAGGATCTGAA+AGG | - | chr2.3:69573317-69573336 | MS.gene54320:CDS | 40.0% | |
| CTGAAAGGAAAACTAGGACT+TGG | - | chr2.3:69573332-69573351 | MS.gene54320:CDS | 40.0% | |
| GAAAGGAAAACTAGGACTTG+GGG | - | chr2.3:69573334-69573353 | MS.gene54320:CDS | 40.0% | |
| GATCCATGGTTTCTGTTGTA+TGG | - | chr2.3:69572737-69572756 | MS.gene54320:CDS | 40.0% | |
| TCACCATACAACAGAAACCA+TGG | + | chr2.3:69572743-69572762 | None:intergenic | 40.0% | |
| TCGTTGTTCCATGCTAAACA+AGG | - | chr2.3:69572772-69572791 | MS.gene54320:CDS | 40.0% | |
| TTGAGTGGCGTGATTAATCA+CGG | + | chr2.3:69573015-69573034 | None:intergenic | 40.0% | |
| TTGTTTAGCATGGAACAACG+AGG | + | chr2.3:69572773-69572792 | None:intergenic | 40.0% | |
| TTTGTCGGGGAGATAATTCA+TGG | - | chr2.3:69573057-69573076 | MS.gene54320:intron | 40.0% | |
| ! | GATTGGCGCTCTAAATTTAG+TGG | - | chr2.3:69572710-69572729 | MS.gene54320:CDS | 40.0% |
| !! | AAAGGGAAAGCATCGATCAA+AGG | - | chr2.3:69573307-69573326 | MS.gene54320:CDS | 40.0% |
| !! | GATGCTCAAAATTTGGTGTG+GGG | - | chr2.3:69573392-69573411 | MS.gene54320:CDS | 40.0% |
| AACAATGCAATCCTGAGACG+TGG | + | chr2.3:69572991-69573010 | None:intergenic | 45.0% | |
| ACAATGCAATCCTGAGACGT+GGG | + | chr2.3:69572990-69573009 | None:intergenic | 45.0% | |
| ACTACACTCGCTCCATTGTT+TGG | + | chr2.3:69573207-69573226 | None:intergenic | 45.0% | |
| ATCGAAATTCCAGAACTGGC+TGG | - | chr2.3:69572812-69572831 | MS.gene54320:CDS | 45.0% | |
| GCACGATCCATACTCCTTTA+AGG | + | chr2.3:69573457-69573476 | None:intergenic | 45.0% | |
| TAGAACTGCACACGCTTTGT+CGG | - | chr2.3:69573042-69573061 | MS.gene54320:intron | 45.0% | |
| TCGAAATTCCAGAACTGGCT+GGG | - | chr2.3:69572813-69572832 | MS.gene54320:CDS | 45.0% | |
| TGGTGTGGGGTGATTACATT+TGG | - | chr2.3:69573405-69573424 | MS.gene54320:CDS | 45.0% | |
| ACTTGCCTTGCATCCTACGA+CGG | - | chr2.3:69572839-69572858 | MS.gene54320:CDS | 50.0% | |
| AGAACTGCACACGCTTTGTC+GGG | - | chr2.3:69573043-69573062 | MS.gene54320:intron | 50.0% | |
| GAACTGCACACGCTTTGTCG+GGG | - | chr2.3:69573044-69573063 | MS.gene54320:intron | 55.0% | |
| TTCATCTGCTCCCACGTCTC+AGG | - | chr2.3:69572977-69572996 | MS.gene54320:intron | 55.0% | |
| ! | CAAGTTGACCCAGCCAGTTC+TGG | + | chr2.3:69572824-69572843 | None:intergenic | 55.0% |
| GAGAAGCAGCCATCCGTCGT+AGG | + | chr2.3:69572855-69572874 | None:intergenic | 60.0% | |
| GCCATCCGTCGTAGGATGCA+AGG | + | chr2.3:69572847-69572866 | None:intergenic | 60.0% | |
| GCCTTGCATCCTACGACGGA+TGG | - | chr2.3:69572843-69572862 | MS.gene54320:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 69572575 | 69573496 | 69572575 | ID=MS.gene54320 |
| chr2.3 | mRNA | 69572575 | 69573496 | 69572575 | ID=MS.gene54320.t1;Parent=MS.gene54320 |
| chr2.3 | exon | 69573180 | 69573496 | 69573180 | ID=MS.gene54320.t1.exon1;Parent=MS.gene54320.t1 |
| chr2.3 | CDS | 69573180 | 69573496 | 69573180 | ID=cds.MS.gene54320.t1;Parent=MS.gene54320.t1 |
| chr2.3 | exon | 69572575 | 69572941 | 69572575 | ID=MS.gene54320.t1.exon2;Parent=MS.gene54320.t1 |
| chr2.3 | CDS | 69572575 | 69572941 | 69572575 | ID=cds.MS.gene54320.t1;Parent=MS.gene54320.t1 |
| Gene Sequence |
| Protein sequence |
