AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54322

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54322.t1 MTR_2g015720 97.596 208 5 0 4 211 153 360 3.46e-151 424
MS.gene54322.t1 MTR_4g087730 75.481 208 51 0 4 211 157 364 2.56e-119 343
MS.gene54322.t1 MTR_4g088030 73.077 208 56 0 4 211 145 352 5.43e-114 329
MS.gene54322.t1 MTR_2g091175 63.285 207 75 1 4 210 162 367 4.55e-99 292
MS.gene54322.t1 MTR_4g079700 62.136 206 77 1 4 209 159 363 1.88e-95 283
MS.gene54322.t1 MTR_3g049330 61.353 207 78 2 5 209 144 350 7.88e-91 270
MS.gene54322.t1 MTR_2g073510 58.049 205 84 2 4 206 146 350 1.13e-87 262
MS.gene54322.t1 MTR_3g021060 55.072 207 91 2 4 209 158 363 3.33e-82 249
MS.gene54322.t1 MTR_3g035830 54.808 208 92 2 3 209 142 348 1.20e-81 247
MS.gene54322.t1 MTR_5g034810 56.250 208 87 3 5 209 146 352 6.73e-80 243
MS.gene54322.t1 MTR_3g021090 54.902 204 90 2 3 205 142 344 4.33e-78 240
MS.gene54322.t1 MTR_3g464570 51.111 225 94 4 2 210 49 273 1.69e-77 234
MS.gene54322.t1 MTR_3g464570 51.111 225 94 4 2 210 153 377 3.90e-76 234
MS.gene54322.t1 MTR_5g071350 48.291 234 95 5 2 210 168 400 7.94e-73 226
MS.gene54322.t1 MTR_2g073500 48.077 208 96 4 4 209 148 345 3.45e-67 210
MS.gene54322.t1 MTR_1g112210 43.636 220 103 6 4 209 157 369 1.49e-58 188
MS.gene54322.t1 MTR_1g112210 43.439 221 104 6 3 209 169 382 1.59e-58 189
MS.gene54322.t1 MTR_1g050448 45.909 220 99 7 3 209 163 375 2.29e-58 188
MS.gene54322.t1 MTR_0136s0010 38.393 224 121 4 4 211 289 511 1.75e-55 184
MS.gene54322.t1 MTR_4g121560 40.271 221 116 5 4 209 234 453 5.21e-54 179
MS.gene54322.t1 MTR_7g093450 39.286 224 119 4 4 211 367 589 7.65e-54 181
MS.gene54322.t1 MTR_1g111010 44.444 207 100 6 5 209 148 341 9.03e-53 173
MS.gene54322.t1 MTR_4g126950 37.838 222 123 5 4 211 228 448 2.43e-50 169
MS.gene54322.t1 MTR_5g059400 36.726 226 125 5 1 209 291 515 8.64e-50 169
MS.gene54322.t1 MTR_1g116130 36.726 226 125 5 1 209 291 515 8.64e-50 169
MS.gene54322.t1 MTR_8g069790 37.500 224 118 5 4 209 297 516 1.27e-47 164
MS.gene54322.t1 MTR_5g033800 36.161 224 121 5 4 209 264 483 3.14e-47 162
MS.gene54322.t1 MTR_7g109550 38.426 216 123 5 2 209 254 467 3.74e-47 161
MS.gene54322.t1 MTR_5g082880 37.445 227 117 6 8 209 285 511 2.02e-43 152
MS.gene54322.t1 MTR_3g070900 36.364 231 122 7 4 209 219 449 2.68e-42 148
MS.gene54322.t1 MTR_4g016940 34.914 232 122 7 4 209 199 427 1.54e-41 145
MS.gene54322.t1 MTR_1g058380 35.909 220 126 5 4 209 176 394 1.05e-40 143
MS.gene54322.t1 MTR_8g463790 36.364 231 119 7 4 210 184 410 1.45e-38 137
MS.gene54322.t1 MTR_3g071650 34.199 231 127 7 4 210 202 431 8.80e-38 135
MS.gene54322.t1 MTR_3g021150 53.043 115 52 2 76 189 1 114 2.73e-37 126
MS.gene54322.t1 MTR_5g083260 34.783 230 127 6 4 210 196 425 8.73e-35 127
MS.gene54322.t1 MTR_4g014440 32.328 232 132 7 4 210 177 408 1.15e-34 127
MS.gene54322.t1 MTR_4g014430 32.751 229 128 7 4 209 221 446 3.20e-34 126
MS.gene54322.t1 MTR_3g090120 35.681 213 123 6 5 209 253 459 1.19e-33 125
MS.gene54322.t1 MTR_2g022560 31.532 222 136 3 4 209 216 437 1.85e-33 124
MS.gene54322.t1 MTR_5g069320 36.957 184 69 4 37 202 232 386 3.98e-33 122
MS.gene54322.t1 MTR_7g070060 31.897 232 133 7 4 210 77 308 7.95e-33 120
MS.gene54322.t1 MTR_7g068920 33.043 230 129 8 4 209 193 421 1.56e-32 121
MS.gene54322.t1 MTR_7g070060 31.897 232 133 7 4 210 179 410 6.50e-32 120
MS.gene54322.t1 MTR_7g094750 33.333 225 129 6 4 209 210 432 5.88e-31 117
MS.gene54322.t1 MTR_8g093530 29.439 214 139 4 4 210 209 417 6.79e-30 114
MS.gene54322.t1 MTR_8g093870 37.572 173 98 7 43 209 304 472 3.12e-22 94.0
MS.gene54322.t1 MTR_4g092830 30.049 203 116 8 23 205 192 388 2.50e-19 85.5
MS.gene54322.t1 MTR_4g125060 35.795 176 97 8 43 209 303 471 3.83e-19 85.1
MS.gene54322.t1 MTR_4g125060 35.795 176 97 8 43 209 371 539 4.14e-19 85.1
MS.gene54322.t1 MTR_4g125060 35.795 176 97 8 43 209 342 510 4.74e-19 85.1
MS.gene54322.t1 MTR_5g033800 33.333 120 69 3 4 115 264 380 1.61e-18 82.8
MS.gene54322.t1 MTR_6g016240 30.342 234 130 11 5 209 187 416 9.77e-18 80.9
MS.gene54322.t1 MTR_3g021080 35.789 95 40 2 116 209 105 179 5.30e-17 75.9
MS.gene54322.t1 MTR_6g016880 28.151 238 133 9 5 211 191 421 1.14e-12 66.2
MS.gene54322.t1 MTR_6g016875 27.004 237 136 10 4 211 179 407 1.98e-12 65.9
MS.gene54322.t1 MTR_6g061710 25.743 202 105 6 43 209 323 514 1.71e-11 63.2
MS.gene54322.t1 MTR_1g076690 28.646 192 104 9 43 205 379 566 2.30e-11 62.8
MS.gene54322.t1 MTR_7g019120 27.979 193 110 7 41 211 237 422 8.16e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54322.t1 AT1G29050 73.206 209 54 2 4 211 173 380 1.18e-114 332
MS.gene54322.t1 AT2G34070 70.000 210 60 3 4 211 43 251 2.64e-110 316
MS.gene54322.t1 AT2G34070 70.000 210 60 3 4 211 177 385 1.41e-108 317
MS.gene54322.t1 AT2G42570 64.734 207 73 0 4 210 159 365 2.60e-100 295
MS.gene54322.t1 AT2G31110 61.650 206 79 0 4 209 9 214 2.86e-97 282
MS.gene54322.t1 AT2G31110 61.650 206 79 0 4 209 157 362 6.29e-96 284
MS.gene54322.t1 AT3G14850 53.398 206 93 2 4 206 43 248 4.82e-80 239
MS.gene54322.t1 AT3G14850 53.398 206 93 2 4 206 146 351 1.29e-78 239
MS.gene54322.t1 AT2G30900 53.110 209 95 2 5 210 158 366 1.55e-77 237
MS.gene54322.t1 AT1G78710 48.077 208 106 2 4 209 149 356 1.16e-74 229
MS.gene54322.t1 AT5G58600 47.768 224 101 4 2 209 177 400 1.51e-67 213
MS.gene54322.t1 AT2G30010 48.472 229 97 4 2 209 168 396 1.98e-67 212
MS.gene54322.t1 AT2G30010 48.472 229 97 4 2 209 211 439 3.00e-67 213
MS.gene54322.t1 AT3G12060 41.964 224 113 5 4 211 315 537 6.94e-57 189
MS.gene54322.t1 AT3G54260 46.606 221 97 7 3 209 161 374 1.10e-55 181
MS.gene54322.t1 AT5G06700 40.179 224 117 4 4 211 374 596 1.17e-55 186
MS.gene54322.t1 AT3G62390 41.553 219 115 5 2 209 255 471 1.11e-53 179
MS.gene54322.t1 AT5G20590 38.739 222 121 5 4 211 261 481 3.81e-52 175
MS.gene54322.t1 AT5G49340 37.220 223 122 5 4 209 220 441 3.41e-48 164
MS.gene54322.t1 AT2G31110 61.905 105 40 0 4 108 157 261 1.26e-45 152
MS.gene54322.t1 AT1G60790 37.168 226 118 6 4 209 311 532 3.14e-45 157
MS.gene54322.t1 AT5G01360 36.207 232 120 7 4 209 149 378 4.53e-42 146
MS.gene54322.t1 AT5G01360 36.207 232 120 7 4 209 205 434 1.18e-41 146
MS.gene54322.t1 AT3G11570 36.364 220 125 6 4 209 201 419 3.29e-41 145
MS.gene54322.t1 AT3G55990 34.199 231 127 6 4 209 253 483 8.68e-40 142
MS.gene54322.t1 AT5G19160 35.111 225 125 4 4 207 218 442 1.79e-39 141
MS.gene54322.t1 AT5G06230 35.023 217 126 5 7 209 149 364 3.61e-39 138
MS.gene54322.t1 AT5G06230 35.023 217 126 5 7 209 190 405 5.00e-39 139
MS.gene54322.t1 AT1G78710 33.654 208 83 3 4 209 142 296 1.41e-38 135
MS.gene54322.t1 AT2G40150 34.177 237 125 6 4 209 184 420 2.88e-38 137
MS.gene54322.t1 AT3G06080 35.242 227 126 8 4 211 222 446 1.88e-37 135
MS.gene54322.t1 AT3G11030 36.325 234 123 8 2 210 217 449 4.31e-37 134
MS.gene54322.t1 AT2G30900 56.731 104 45 0 5 108 158 261 1.26e-36 129
MS.gene54322.t1 AT1G73140 33.913 230 124 7 4 209 180 405 7.56e-36 130
MS.gene54322.t1 AT2G40320 34.914 232 124 8 4 210 194 423 5.18e-35 128
MS.gene54322.t1 AT5G01620 32.018 228 131 7 4 209 232 457 2.40e-34 127
MS.gene54322.t1 AT5G01620 32.018 228 131 7 4 209 224 449 2.61e-34 127
MS.gene54322.t1 AT5G01620 32.018 228 131 7 4 209 224 449 2.61e-34 127
MS.gene54322.t1 AT2G40160 31.780 236 131 7 4 211 190 423 2.82e-34 126
MS.gene54322.t1 AT5G58600 49.558 113 56 1 2 113 177 289 2.98e-34 124
MS.gene54322.t1 AT2G40160 31.739 230 129 6 4 205 190 419 4.28e-34 126
MS.gene54322.t1 AT1G48880 33.803 213 127 4 5 210 235 440 2.64e-33 124
MS.gene54322.t1 AT2G38320 32.328 232 132 6 3 209 90 321 5.97e-33 121
MS.gene54322.t1 AT2G38320 32.328 232 132 6 3 209 173 404 1.90e-32 121
MS.gene54322.t1 AT3G62390 37.500 120 70 2 2 116 255 374 2.11e-24 99.8
MS.gene54322.t1 AT5G64470 31.472 197 120 6 22 205 196 390 3.63e-22 93.2
MS.gene54322.t1 AT5G20590 38.136 118 67 3 4 115 261 378 1.98e-21 91.3
MS.gene54322.t1 AT5G64470 31.373 204 117 7 22 205 196 396 3.42e-21 90.9
MS.gene54322.t1 AT5G19160 34.454 119 71 2 4 115 218 336 4.31e-19 84.3
MS.gene54322.t1 AT2G14530 30.769 182 106 7 44 210 231 407 1.03e-18 84.0
MS.gene54322.t1 AT5G64020 33.155 187 99 10 43 209 228 408 1.09e-17 80.9
MS.gene54322.t1 AT5G20680 33.143 175 103 8 43 209 289 457 5.70e-17 79.0
MS.gene54322.t1 AT5G20680 33.143 175 103 8 43 209 365 533 5.83e-17 79.0
MS.gene54322.t1 AT5G20680 33.143 175 103 8 43 209 383 551 5.86e-17 79.0
MS.gene54322.t1 AT5G20680 33.143 175 103 8 43 209 383 551 5.86e-17 79.0
MS.gene54322.t1 AT5G20680 33.143 175 103 8 43 209 383 551 5.86e-17 79.0
MS.gene54322.t1 AT5G20680 33.143 175 103 8 43 209 383 551 5.86e-17 79.0
MS.gene54322.t1 AT5G06230 35.345 116 68 2 7 115 190 305 1.09e-16 77.4
MS.gene54322.t1 AT3G06080 31.092 119 75 2 4 115 222 340 2.14e-15 73.9
MS.gene54322.t1 AT2G37720 32.967 182 90 9 45 209 316 482 6.55e-15 73.2
MS.gene54322.t1 AT3G02440 30.000 150 91 3 73 209 328 476 4.76e-14 70.5
MS.gene54322.t1 AT3G02440 30.000 150 91 3 73 209 320 468 5.05e-14 70.5
MS.gene54322.t1 AT2G40160 31.405 121 72 3 4 113 190 310 5.00e-13 67.0
MS.gene54322.t1 AT1G70230 26.407 231 132 11 5 209 193 411 1.08e-12 66.6

Find 56 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTATACCGCACACCTTATT+TGG 0.110333 2.3:+69648996 MS.gene54322:CDS
ACCCCTGTGATGATCCTTTA+TGG 0.200502 2.3:-69649131 None:intergenic
GATTGACCACTTGGGCTAAA+TGG 0.217574 2.3:+69649720 MS.gene54322:CDS
CGAATTATGTCAACCAAATA+AGG 0.257968 2.3:-69649009 None:intergenic
GGACAACTTCAACCATTATC+AGG 0.281304 2.3:+69649977 MS.gene54322:CDS
CCGTTTAGTGGCATACAATA+AGG 0.283673 2.3:+69649697 MS.gene54322:CDS
TTGAAAGAAGACTTTAGTTT+TGG 0.332563 2.3:-69649765 None:intergenic
TTGTTCAATATGTTACTTGA+TGG 0.341330 2.3:-69650025 None:intergenic
AAAACTAAAGTCTTCTTTCA+AGG 0.374728 2.3:+69649767 MS.gene54322:CDS
GGTGTATTTGTGCAGGACTA+TGG 0.375908 2.3:+69648961 MS.gene54322:intron
AGGAGTTGGTTCCAAGTATC+AGG 0.377013 2.3:-69650187 None:intergenic
GTAATATCAAGTAGATAAAC+TGG 0.378242 2.3:-69650070 None:intergenic
TGGCATTGGTGGACCCATAA+AGG 0.406028 2.3:+69649117 MS.gene54322:CDS
CCCCTGTGATGATCCTTTAT+GGG 0.409627 2.3:-69649130 None:intergenic
TACCAGGATTACCTGATACT+TGG 0.421208 2.3:+69650176 MS.gene54322:CDS
CGTTTAGTGGCATACAATAA+GGG 0.422401 2.3:+69649698 MS.gene54322:CDS
TGCAGCCATTGGTGCTTACC+AGG 0.425703 2.3:+69650160 MS.gene54322:CDS
ATTGACCACTTGGGCTAAAT+GGG 0.430670 2.3:+69649721 MS.gene54322:CDS
CAAGAACATGGACCGTTTAG+TGG 0.437683 2.3:+69649685 MS.gene54322:CDS
GGATATGTTGACCCTGATAA+TGG 0.441283 2.3:-69649989 None:intergenic
ACAATAAGGGATTGACCACT+TGG 0.475609 2.3:+69649711 MS.gene54322:CDS
GCCTGCATGATCACCACTAT+AGG 0.481631 2.3:-69650131 None:intergenic
CCTTATTGTATGCCACTAAA+CGG 0.487673 2.3:-69649697 None:intergenic
ACATGTAGAGTGCAGCATAT+AGG 0.498513 2.3:-69650207 None:intergenic
GCACACCCTTCTTCCTATAG+TGG 0.508415 2.3:+69650118 MS.gene54322:CDS
CAGGCAATGATTGCAGCCAT+TGG 0.512288 2.3:+69650149 MS.gene54322:CDS
TTACACTTCTCTCACAACTA+AGG 0.518338 2.3:+69650089 MS.gene54322:CDS
AGATGGGATTATATAAGAGA+TGG 0.530008 2.3:+69649650 MS.gene54322:intron
TTGTGACTTGAACAGGGGAA+AGG 0.533258 2.3:+69649925 MS.gene54322:intron
TGATCACCACTATAGGAAGA+AGG 0.537215 2.3:-69650124 None:intergenic
GTAATCCTGGTAAGCACCAA+TGG 0.537271 2.3:-69650165 None:intergenic
TCCTATAGTGGTGATCATGC+AGG 0.537608 2.3:+69650130 MS.gene54322:CDS
ACATTGGACTCTATTGTCGC+TGG 0.539178 2.3:+69649054 MS.gene54322:CDS
TTCCAAGTATCAGGTAATCC+TGG 0.542149 2.3:-69650178 None:intergenic
GACCCATAAAGGATCATCAC+AGG 0.551126 2.3:+69649128 MS.gene54322:CDS
TTCAATATGTTACTTGATGG+AGG 0.553250 2.3:-69650022 None:intergenic
CTTGATGGAGGTAGACCTGC+TGG 0.559776 2.3:-69650010 None:intergenic
GATGGGATTATATAAGAGAT+GGG 0.572464 2.3:+69649651 MS.gene54322:intron
ATCAACCCATTTAGCCCAAG+TGG 0.578202 2.3:-69649726 None:intergenic
CGTTGGACGAGTGCTTACAT+TGG 0.580963 2.3:+69649038 MS.gene54322:CDS
GTCGCTGGAAATGCATGGAA+AGG 0.589762 2.3:+69649069 MS.gene54322:CDS
AGAGTGCAGCATATAGGAGT+TGG 0.590451 2.3:-69650201 None:intergenic
TCAGGGTCAACATATCCAGC+AGG 0.593746 2.3:+69649995 MS.gene54322:CDS
GACATAATTCGAGAAAACGT+TGG 0.597443 2.3:+69649021 MS.gene54322:CDS
TGGAAATGCATGGAAAGGAA+TGG 0.604996 2.3:+69649074 MS.gene54322:CDS
GATCACCACTATAGGAAGAA+GGG 0.614202 2.3:-69650123 None:intergenic
ATGGAAAGGAATGGACATGC+TGG 0.621693 2.3:+69649083 MS.gene54322:CDS
GACAACTTCAACCATTATCA+GGG 0.625022 2.3:+69649978 MS.gene54322:CDS
CTATTGTCGCTGGAAATGCA+TGG 0.626945 2.3:+69649064 MS.gene54322:CDS
ACTTGAACAGGGGAAAGGAG+TGG 0.645093 2.3:+69649930 MS.gene54322:intron
GGCACTTACTGATAATGAGT+AGG 0.647065 2.3:-69649797 None:intergenic
GTCTAAACTAGTCAAGAACA+TGG 0.648323 2.3:+69649673 MS.gene54322:CDS
ACCCATAAAGGATCATCACA+GGG 0.652199 2.3:+69649129 MS.gene54322:CDS
TGTCAACCAAATAAGGTGTG+CGG 0.666261 2.3:-69649002 None:intergenic
CAATAAGGGATTGACCACTT+GGG 0.666755 2.3:+69649712 MS.gene54322:CDS
CCCATAAAGGATCATCACAG+GGG 0.769221 2.3:+69649130 MS.gene54322:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAATTAAAATTTTATTAA+TGG - chr2.3:69649580-69649599 None:intergenic 0.0%
!! AGAAAAAAAAAAACATTACT+TGG + chr2.3:69649603-69649622 MS.gene54322:intron 15.0%
!! CTATATAAATTACTTTCTAA+CGG + chr2.3:69649271-69649290 MS.gene54322:intron 15.0%
!!! AATTTTATTAATGGAAGTAT+TGG - chr2.3:69649571-69649590 None:intergenic 15.0%
!!! AGTAATTTATATAGTTCTTT+TGG - chr2.3:69649265-69649284 None:intergenic 15.0%
!!! GTAATTTATATAGTTCTTTT+GGG - chr2.3:69649264-69649283 None:intergenic 15.0%
!!! TTAATTTATTTTCAGCATTA+TGG - chr2.3:69649887-69649906 None:intergenic 15.0%
!! CATTATGGAATATACAAAAA+TGG - chr2.3:69649872-69649891 None:intergenic 20.0%
!!! TCGTGATTTTGTAAATATTA+CGG + chr2.3:69649446-69649465 MS.gene54322:intron 20.0%
!!! TTTGTTTTTTGGTGAATAAA+TGG - chr2.3:69649316-69649335 None:intergenic 20.0%
! AAAACTAAAGTCTTCTTTCA+AGG + chr2.3:69649767-69649786 MS.gene54322:CDS 25.0%
! AAGCATACACGTAAAATATT+AGG - chr2.3:69649389-69649408 None:intergenic 25.0%
! ACACAAAACTATTGAATAGA+AGG - chr2.3:69649821-69649840 None:intergenic 25.0%
! GTAATATCAAGTAGATAAAC+TGG - chr2.3:69650073-69650092 None:intergenic 25.0%
! TATTAAAGAGTGATAACAGT+TGG + chr2.3:69648934-69648953 MS.gene54322:intron 25.0%
!! AATATTTTGTGACTTGAACA+GGG + chr2.3:69649919-69649938 MS.gene54322:intron 25.0%
!! TAATATTTTACGTGTATGCT+TGG + chr2.3:69649388-69649407 MS.gene54322:intron 25.0%
!! TAATATTTTGTGACTTGAAC+AGG + chr2.3:69649918-69649937 MS.gene54322:intron 25.0%
!! TACTTTTATCATGTTCAGAT+GGG + chr2.3:69649634-69649653 MS.gene54322:intron 25.0%
!! TTGTTCAATATGTTACTTGA+TGG - chr2.3:69650028-69650047 None:intergenic 25.0%
!!! ACTATGTTTACAGTTTTGAT+TGG + chr2.3:69649344-69649363 MS.gene54322:intron 25.0%
!!! AGTGTACATACTTTGTTTTT+TGG - chr2.3:69649327-69649346 None:intergenic 25.0%
!!! CTATGTTTACAGTTTTGATT+GGG + chr2.3:69649345-69649364 MS.gene54322:intron 25.0%
!!! TTGAAAGAAGACTTTAGTTT+TGG - chr2.3:69649768-69649787 None:intergenic 25.0%
AGATGGGATTATATAAGAGA+TGG + chr2.3:69649650-69649669 MS.gene54322:intron 30.0%
CAAACATACTGTTACTAGTT+AGG - chr2.3:69649227-69649246 None:intergenic 30.0%
CAACCAAAAAATAGTTGTAG+TGG + chr2.3:69649956-69649975 MS.gene54322:CDS 30.0%
CGAATTATGTCAACCAAATA+AGG - chr2.3:69649012-69649031 None:intergenic 30.0%
CTAACTAGTAACAGTATGTT+TGG + chr2.3:69649225-69649244 MS.gene54322:intron 30.0%
GATGGGATTATATAAGAGAT+GGG + chr2.3:69649651-69649670 MS.gene54322:intron 30.0%
TGTTATCACTCTTTAATAGC+TGG - chr2.3:69648932-69648951 None:intergenic 30.0%
! ATATTTTGTGACTTGAACAG+GGG + chr2.3:69649920-69649939 MS.gene54322:intron 30.0%
! TTCAATATGTTACTTGATGG+AGG - chr2.3:69650025-69650044 None:intergenic 30.0%
!! CTACTTTTATCATGTTCAGA+TGG + chr2.3:69649633-69649652 MS.gene54322:intron 30.0%
!!! AAGAAGACTTTAGTTTTGGT+AGG - chr2.3:69649764-69649783 None:intergenic 30.0%
!!! TGTCCACTACAACTATTTTT+TGG - chr2.3:69649962-69649981 None:intergenic 30.0%
CCTTATTGTATGCCACTAAA+CGG - chr2.3:69649700-69649719 None:intergenic 35.0%
CGTTTAGTGGCATACAATAA+GGG + chr2.3:69649698-69649717 MS.gene54322:CDS 35.0%
GACAACTTCAACCATTATCA+GGG + chr2.3:69649978-69649997 MS.gene54322:CDS 35.0%
GACATAATTCGAGAAAACGT+TGG + chr2.3:69649021-69649040 MS.gene54322:CDS 35.0%
GTCTAAACTAGTCAAGAACA+TGG + chr2.3:69649673-69649692 MS.gene54322:CDS 35.0%
TACATATGTGGTAGCAATTG+CGG + chr2.3:69649516-69649535 MS.gene54322:intron 35.0%
TGGTATTAAATTGCATCGCA+TGG - chr2.3:69648912-69648931 None:intergenic 35.0%
TTACACTTCTCTCACAACTA+AGG + chr2.3:69650089-69650108 MS.gene54322:CDS 35.0%
!! AATGGAAGTATTGGTCTACA+TGG - chr2.3:69649562-69649581 None:intergenic 35.0%
ACAATAAGGGATTGACCACT+TGG + chr2.3:69649711-69649730 MS.gene54322:CDS 40.0%
ACATGTAGAGTGCAGCATAT+AGG - chr2.3:69650210-69650229 None:intergenic 40.0%
ACCCATAAAGGATCATCACA+GGG + chr2.3:69649129-69649148 MS.gene54322:CDS 40.0%
ACTATACCGCACACCTTATT+TGG + chr2.3:69648996-69649015 MS.gene54322:CDS 40.0%
ACTGTTACTAGTTAGGTGTG+TGG - chr2.3:69649220-69649239 None:intergenic 40.0%
ATTGACCACTTGGGCTAAAT+GGG + chr2.3:69649721-69649740 MS.gene54322:CDS 40.0%
CAATAAGGGATTGACCACTT+GGG + chr2.3:69649712-69649731 MS.gene54322:CDS 40.0%
CCAAACATAGGCTACATATG+TGG + chr2.3:69649504-69649523 MS.gene54322:intron 40.0%
CCACATATGTAGCCTATGTT+TGG - chr2.3:69649507-69649526 None:intergenic 40.0%
CCGTTTAGTGGCATACAATA+AGG + chr2.3:69649697-69649716 MS.gene54322:CDS 40.0%
CGGTGGATATGTTAAAAGCA+CGG - chr2.3:69649479-69649498 None:intergenic 40.0%
CTGTTACTAGTTAGGTGTGT+GGG - chr2.3:69649219-69649238 None:intergenic 40.0%
GATCACCACTATAGGAAGAA+GGG - chr2.3:69650126-69650145 None:intergenic 40.0%
GGACAACTTCAACCATTATC+AGG + chr2.3:69649977-69649996 MS.gene54322:CDS 40.0%
GGATATGTTGACCCTGATAA+TGG - chr2.3:69649992-69650011 None:intergenic 40.0%
GTAGCCTATGTTTGGTATCA+CGG - chr2.3:69649499-69649518 None:intergenic 40.0%
TACCAGGATTACCTGATACT+TGG + chr2.3:69650176-69650195 MS.gene54322:CDS 40.0%
TACGTGTATGCTTGGTATCA+CGG + chr2.3:69649396-69649415 MS.gene54322:intron 40.0%
TGATCACCACTATAGGAAGA+AGG - chr2.3:69650127-69650146 None:intergenic 40.0%
TGGAAATGCATGGAAAGGAA+TGG + chr2.3:69649074-69649093 MS.gene54322:CDS 40.0%
TGTCAACCAAATAAGGTGTG+CGG - chr2.3:69649005-69649024 None:intergenic 40.0%
TTCCAAGTATCAGGTAATCC+TGG - chr2.3:69650181-69650200 None:intergenic 40.0%
! AGAGTGATAACAGTTGGTGT+TGG + chr2.3:69648940-69648959 MS.gene54322:intron 40.0%
! GGCACTTACTGATAATGAGT+AGG - chr2.3:69649800-69649819 None:intergenic 40.0%
! TGGTTTTCAACTCATGGCAT+TGG + chr2.3:69649103-69649122 MS.gene54322:CDS 40.0%
!! TTTTCAACTCATGGCATTGG+TGG + chr2.3:69649106-69649125 MS.gene54322:CDS 40.0%
!!! ACATGCTGGTTTTCAACTCA+TGG + chr2.3:69649097-69649116 MS.gene54322:CDS 40.0%
ACATTGGACTCTATTGTCGC+TGG + chr2.3:69649054-69649073 MS.gene54322:CDS 45.0%
ACCCCTGTGATGATCCTTTA+TGG - chr2.3:69649134-69649153 None:intergenic 45.0%
AGAGTGCAGCATATAGGAGT+TGG - chr2.3:69650204-69650223 None:intergenic 45.0%
ATCAACCCATTTAGCCCAAG+TGG - chr2.3:69649729-69649748 None:intergenic 45.0%
ATGGAAAGGAATGGACATGC+TGG + chr2.3:69649083-69649102 MS.gene54322:CDS 45.0%
CAAGAACATGGACCGTTTAG+TGG + chr2.3:69649685-69649704 MS.gene54322:CDS 45.0%
CCCATAAAGGATCATCACAG+GGG + chr2.3:69649130-69649149 MS.gene54322:CDS 45.0%
CCCCTGTGATGATCCTTTAT+GGG - chr2.3:69649133-69649152 None:intergenic 45.0%
CTATTGTCGCTGGAAATGCA+TGG + chr2.3:69649064-69649083 MS.gene54322:CDS 45.0%
GACCCATAAAGGATCATCAC+AGG + chr2.3:69649128-69649147 MS.gene54322:CDS 45.0%
GATTGACCACTTGGGCTAAA+TGG + chr2.3:69649720-69649739 MS.gene54322:CDS 45.0%
GGTGTATTTGTGCAGGACTA+TGG + chr2.3:69648961-69648980 MS.gene54322:intron 45.0%
GTAATCCTGGTAAGCACCAA+TGG - chr2.3:69650168-69650187 None:intergenic 45.0%
TCCACCGTGATACCAAACAT+AGG + chr2.3:69649492-69649511 MS.gene54322:intron 45.0%
TCCTATAGTGGTGATCATGC+AGG + chr2.3:69650130-69650149 MS.gene54322:CDS 45.0%
TTGATTGGGATGCGATTGCA+TGG + chr2.3:69649359-69649378 MS.gene54322:intron 45.0%
TTGTGACTTGAACAGGGGAA+AGG + chr2.3:69649925-69649944 MS.gene54322:intron 45.0%
! AGGAGTTGGTTCCAAGTATC+AGG - chr2.3:69650190-69650209 None:intergenic 45.0%
!! TGGTGTTGGTGTATTTGTGC+AGG + chr2.3:69648954-69648973 MS.gene54322:intron 45.0%
ACTTGAACAGGGGAAAGGAG+TGG + chr2.3:69649930-69649949 MS.gene54322:intron 50.0%
CAGGCAATGATTGCAGCCAT+TGG + chr2.3:69650149-69650168 MS.gene54322:CDS 50.0%
CGTTGGACGAGTGCTTACAT+TGG + chr2.3:69649038-69649057 MS.gene54322:CDS 50.0%
GCACACCCTTCTTCCTATAG+TGG + chr2.3:69650118-69650137 MS.gene54322:CDS 50.0%
GCCTATGTTTGGTATCACGG+TGG - chr2.3:69649496-69649515 None:intergenic 50.0%
GCCTGCATGATCACCACTAT+AGG - chr2.3:69650134-69650153 None:intergenic 50.0%
GTCGCTGGAAATGCATGGAA+AGG + chr2.3:69649069-69649088 MS.gene54322:CDS 50.0%
GTGTATGCTTGGTATCACGG+TGG + chr2.3:69649399-69649418 MS.gene54322:intron 50.0%
TCAGGGTCAACATATCCAGC+AGG + chr2.3:69649995-69650014 MS.gene54322:CDS 50.0%
!! TGGCATTGGTGGACCCATAA+AGG + chr2.3:69649117-69649136 MS.gene54322:CDS 50.0%
CTTGATGGAGGTAGACCTGC+TGG - chr2.3:69650013-69650032 None:intergenic 55.0%
! TGCAGCCATTGGTGCTTACC+AGG + chr2.3:69650160-69650179 MS.gene54322:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 69648878 69650231 69648878 ID=MS.gene54322
chr2.3 mRNA 69648878 69650231 69648878 ID=MS.gene54322.t1;Parent=MS.gene54322
chr2.3 exon 69648878 69648886 69648878 ID=MS.gene54322.t1.exon1;Parent=MS.gene54322.t1
chr2.3 CDS 69648878 69648886 69648878 ID=cds.MS.gene54322.t1;Parent=MS.gene54322.t1
chr2.3 exon 69648976 69649151 69648976 ID=MS.gene54322.t1.exon2;Parent=MS.gene54322.t1
chr2.3 CDS 69648976 69649151 69648976 ID=cds.MS.gene54322.t1;Parent=MS.gene54322.t1
chr2.3 exon 69649652 69649810 69649652 ID=MS.gene54322.t1.exon3;Parent=MS.gene54322.t1
chr2.3 CDS 69649652 69649810 69649652 ID=cds.MS.gene54322.t1;Parent=MS.gene54322.t1
chr2.3 exon 69649940 69650231 69649940 ID=MS.gene54322.t1.exon4;Parent=MS.gene54322.t1
chr2.3 CDS 69649940 69650231 69649940 ID=cds.MS.gene54322.t1;Parent=MS.gene54322.t1
Gene Sequence

>MS.gene54322

ATGTTGTTTGTTAGTGGTTTGTGAAACATTTCCATGCGATGCAATTTAATACCAGCTATTAAAGAGTGATAACAGTTGGTGTTGGTGTATTTGTGCAGGACTATGGTGTAACTATACAACTATACCGCACACCTTATTTGGTTGACATAATTCGAGAAAACGTTGGACGAGTGCTTACATTGGACTCTATTGTCGCTGGAAATGCATGGAAAGGAATGGACATGCTGGTTTTCAACTCATGGCATTGGTGGACCCATAAAGGATCATCACAGGGGTATGCTTTGCTTTTTGTACAATCATAATCATTCTACTTATAGCACAACAACAATATTCACACACCCACACACCTAACTAGTAACAGTATGTTTGGTAGCAATGTTTATCCCAAAAGAACTATATAAATTACTTTCTAACGGTAAATCAAACATAACATCACCATTTATTCACCAAAAAACAAAGTATGTACACTATGTTTACAGTTTTGATTGGGATGCGATTGCATGGATCACCTAATATTTTACGTGTATGCTTGGTATCACGGTGGATATGTCAGAATCACAATGATTCGTCGTGATTTTGTAAATATTACGGTGAATCACCGTGCTTTTAACATATCCACCGTGATACCAAACATAGGCTACATATGTGGTAGCAATTGCGGTTGCGAGTTGCAATTCAAAACCATGTAGACCAATACTTCCATTAATAAAATTTTAATTATAGAAAGAAAAAAAAAAACATTACTTGGTTTCTAACTACTTTTATCATGTTCAGATGGGATTATATAAGAGATGGGTCTAAACTAGTCAAGAACATGGACCGTTTAGTGGCATACAATAAGGGATTGACCACTTGGGCTAAATGGGTTGATCTCAATGTTGATCCTACCAAAACTAAAGTCTTCTTTCAAGGCGTTTCTCCTACTCATTATCAGTAAGTGCCTTCTATTCAATAGTTTTGTGTAATAAATTTATATTAGTAAAGCTTAAATCCATTTTTGTATATTCCATAATGCTGAAAATAAATTAAAACAAAATTTATAATATTTTGTGACTTGAACAGGGGAAAGGAGTGGAATCAACCAAAAAATAGTTGTAGTGGACAACTTCAACCATTATCAGGGTCAACATATCCAGCAGGTCTACCTCCATCAAGTAACATATTGAACAATGTATTGAAGAGTATGAAAACACCAGTTTATCTACTTGATATTACACTTCTCTCACAACTAAGGAAAGATGCACACCCTTCTTCCTATAGTGGTGATCATGCAGGCAATGATTGCAGCCATTGGTGCTTACCAGGATTACCTGATACTTGGAACCAACTCCTATATGCTGCACTCTACATGTGA
Protein sequence

>MS.gene54322.t1

MLFDYGVTIQLYRTPYLVDIIRENVGRVLTLDSIVAGNAWKGMDMLVFNSWHWWTHKGSSQGWDYIRDGSKLVKNMDRLVAYNKGLTTWAKWVDLNVDPTKTKVFFQGVSPTHYQGKEWNQPKNSCSGQLQPLSGSTYPAGLPPSSNILNNVLKSMKTPVYLLDITLLSQLRKDAHPSSYSGDHAGNDCSHWCLPGLPDTWNQLLYAALYM