AlfalfaGEDB | MS.gene54825

M. sativa cultivar XinJiangDaYe / MS.gene54825

KEGG: -
GO: -
PFAM: PF04852.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54825.t1	XP_003597127.2	100	167	0	0	1	167	1	167	3.60E-92	347.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54825.t1	Q9S7R3	86.0	143	19	1	5	147	8	149	1.6e-69	263.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54825.t1	G7IU28	100.0	167	0	0	1	167	1	167	2.6e-92	347.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051648	MS.gene54825	0.826282	2.95E-54	-1.69E-46
MS.gene052150	MS.gene54825	0.811688	6.18E-51	-1.69E-46
MS.gene052151	MS.gene54825	0.830405	2.99E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54825.t1	MTR_2g092950	100.000	167	0	0	1	167	1	167	2.84e-126	351
MS.gene54825.t1	MTR_4g079930	86.713	143	18	1	5	146	10	152	1.14e-93	269
MS.gene54825.t1	MTR_2g076400	78.846	156	23	1	1	146	1	156	1.69e-89	259
MS.gene54825.t1	MTR_2g016110	76.433	157	32	2	11	162	20	176	5.81e-83	243
MS.gene54825.t1	MTR_4g094428	70.345	145	42	1	21	164	44	188	7.23e-73	219
MS.gene54825.t1	MTR_5g072510	71.970	132	37	0	20	151	34	165	1.04e-72	218
MS.gene54825.t1	MTR_8g086350	83.333	126	21	0	21	146	29	154	7.12e-70	209
MS.gene54825.t1	MTR_1g442860	76.800	125	29	0	23	147	65	189	7.82e-70	210
MS.gene54825.t1	MTR_7g115700	65.306	147	46	3	12	155	16	160	4.72e-66	199
MS.gene54825.t1	MTR_1g075990	76.515	132	31	0	20	151	50	181	7.77e-66	199
MS.gene54825.t1	MTR_7g097030	77.692	130	29	0	22	151	63	192	5.90e-64	196
MS.gene54825.t1	MTR_3g031830	71.127	142	41	0	10	151	29	170	7.16e-64	196
MS.gene54825.t1	MTR_1g069825	77.519	129	29	0	23	151	52	180	2.28e-63	194
MS.gene54825.t1	MTR_1g080210	73.600	125	33	0	23	147	40	164	1.89e-58	180
MS.gene54825.t1	MTR_1g114020	63.636	55	17	1	12	66	63	114	6.25e-16	71.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54825.t1	AT2G42610	85.915	142	19	1	5	146	8	148	1.41e-89	259
MS.gene54825.t1	AT2G42610	85.915	142	19	1	5	146	8	148	1.41e-89	259
MS.gene54825.t1	AT1G07090	71.533	137	39	0	15	151	23	159	6.81e-74	220
MS.gene54825.t1	AT1G78815	75.591	127	31	0	20	146	37	163	5.62e-73	218
MS.gene54825.t1	AT4G18610	76.984	126	28	1	22	146	36	161	2.51e-71	213
MS.gene54825.t1	AT5G58500	74.046	131	33	1	22	151	18	148	6.16e-71	212
MS.gene54825.t1	AT1G16910	68.531	143	45	0	20	162	20	162	2.24e-70	210
MS.gene54825.t1	AT2G31160	76.744	129	30	0	23	151	54	182	2.08e-64	197
MS.gene54825.t1	AT3G23290	77.953	127	28	0	21	147	46	172	1.30e-63	194
MS.gene54825.t1	AT5G28490	74.627	134	34	0	22	155	24	157	1.89e-63	193
MS.gene54825.t1	AT3G04510	71.970	132	37	0	23	154	33	164	4.34e-62	190

Find 49 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTAGGTATTTAGATCAATT+TGG	0.234689	2.1:-9447986	MS.gene54825:CDS
ACCATGTGTTGGAATTTCTT+AGG	0.249818	2.1:-9448003	MS.gene54825:CDS
GGAAGCCTTGATGCTCTTAT+TGG	0.251412	2.1:-9447875	MS.gene54825:CDS
ATGCTCTTATTGGGAGGTTA+AGG	0.286320	2.1:-9447865	MS.gene54825:CDS
AGAAGAGATTGGAACACTTT+TGG	0.305989	2.1:-9448079	MS.gene54825:CDS
GAAGCCTTGATGCTCTTATT+GGG	0.358150	2.1:-9447874	MS.gene54825:CDS
TGCTCTTATTGGGAGGTTAA+GGG	0.383201	2.1:-9447864	MS.gene54825:CDS
CGTTTATCTTAGAGAGGTTA+GGG	0.392703	2.1:-9447780	MS.gene54825:CDS
GTACAAGGTGCTGGTGGCTC+TGG	0.394151	2.1:+9447920	None:intergenic
GCGTTTATCTTAGAGAGGTT+AGG	0.401453	2.1:-9447781	MS.gene54825:CDS
ATGTCCACTTAGACAAGCTT+GGG	0.408374	2.1:-9447897	MS.gene54825:CDS
AAAGTGGAACGGGTGGTCTA+AGG	0.420057	2.1:+9448041	None:intergenic
AATCTCAAAAGAGAAGAGAT+TGG	0.439992	2.1:-9448090	MS.gene54825:CDS
ACAACCTTGTAAGTGAACTT+TGG	0.477909	2.1:+9447958	None:intergenic
TTGCAGTGAGAAAGTGGAAC+GGG	0.495114	2.1:+9448031	None:intergenic
CTTCCCCAAGCTTGTCTAAG+TGG	0.503711	2.1:+9447893	None:intergenic
CATGTCCACTTAGACAAGCT+TGG	0.511810	2.1:-9447898	MS.gene54825:CDS
AAAACCAAAGTTCACTTACA+AGG	0.520249	2.1:-9447962	MS.gene54825:CDS
GGGAGTGTCAAGCTAAGGCT+AGG	0.530004	2.1:-9447760	MS.gene54825:CDS
AAACCAAATATGTCTTCAAG+TGG	0.542706	2.1:-9448172	None:intergenic
AAGAAATTCCAACACATGGT+TGG	0.545252	2.1:+9448006	None:intergenic
GAGGTTAAGGGCTGCATATG+AGG	0.551450	2.1:-9447852	MS.gene54825:CDS
TGTCTAAGTGGACATGTACA+AGG	0.553070	2.1:+9447905	None:intergenic
TGAGGAAAACGGTGGAACTC+CGG	0.553996	2.1:-9447834	MS.gene54825:CDS
CTGCAATTCCAACCATGTGT+TGG	0.557019	2.1:-9448014	MS.gene54825:CDS
AGTGGAAAGGATTTAGCTGA+AGG	0.563487	2.1:-9448154	MS.gene54825:CDS
GGTTAGGGAGTGTCAAGCTA+AGG	0.565244	2.1:-9447765	MS.gene54825:CDS
GGAGTGTCAAGCTAAGGCTA+GGG	0.568004	2.1:-9447759	MS.gene54825:CDS
AAATATGTCTTCAAGTGGAA+AGG	0.571184	2.1:-9448167	None:intergenic
CTCGCGAAAGGATTAGTCTC+CGG	0.573225	2.1:+9447815	None:intergenic
AGGGCTGCATATGAGGAAAA+CGG	0.576049	2.1:-9447845	MS.gene54825:CDS
CGAATAGCACCGCTCGCGAA+AGG	0.579160	2.1:+9447803	None:intergenic
TTGGAATTGCAGTGAGAAAG+TGG	0.579943	2.1:+9448025	None:intergenic
GGAGGATCATCAACCTCGCA+AGG	0.584784	2.1:-9448133	MS.gene54825:CDS
GCCTTGATGCTCTTATTGGG+AGG	0.590380	2.1:-9447871	MS.gene54825:CDS
GCTAAGTGTGATTCCTTGCG+AGG	0.590859	2.1:+9448120	None:intergenic
ACCTCCCAATAAGAGCATCA+AGG	0.595384	2.1:+9447870	None:intergenic
AGTGGACATGTACAAGGTGC+TGG	0.597857	2.1:+9447911	None:intergenic
TATTCGCGTTTATCTTAGAG+AGG	0.607515	2.1:-9447786	MS.gene54825:CDS
ACCTAAGAAATTCCAACACA+TGG	0.612951	2.1:+9448002	None:intergenic
GGAAATTCAAGAAAAGTGCA+TGG	0.626006	2.1:+9447676	None:intergenic
GGAAAGGATTTAGCTGAAGG+AGG	0.626283	2.1:-9448151	MS.gene54825:CDS
GAGACTAATCCTTTCGCGAG+CGG	0.631568	2.1:-9447812	MS.gene54825:CDS
CAGTGAGAAAGTGGAACGGG+TGG	0.652107	2.1:+9448034	None:intergenic
GAGTGTCAAGCTAAGGCTAG+GGG	0.673844	2.1:-9447758	MS.gene54825:CDS
GGACATGTACAAGGTGCTGG+TGG	0.708995	2.1:+9447914	None:intergenic
TGTCCACTTAGACAAGCTTG+GGG	0.716611	2.1:-9447896	MS.gene54825:CDS
ATTGCAGTGAGAAAGTGGAA+CGG	0.719471	2.1:+9448030	None:intergenic
GCTGCATATGAGGAAAACGG+TGG	0.735412	2.1:-9447842	MS.gene54825:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CTTAGGTATTTAGATCAATT+TGG	-	chr2.1:9447859-9447878	MS.gene54825:CDS	25.0%
!!	AAAAAGAAGAGCACAAATAA+GGG	-	chr2.1:9448126-9448145	MS.gene54825:CDS	25.0%
!!	TCTTCTTTTTCTTCTTGTAA+GGG	+	chr2.1:9448116-9448135	None:intergenic	25.0%
!!	TTACAAGGTTGTATGTTTTA+TGG	-	chr2.1:9447898-9447917	MS.gene54825:CDS	25.0%
	AAAACCAAAGTTCACTTACA+AGG	-	chr2.1:9447883-9447902	MS.gene54825:CDS	30.0%
	AATCTCAAAAGAGAAGAGAT+TGG	-	chr2.1:9447755-9447774	MS.gene54825:CDS	30.0%
!	CTCTTCTTTTTCTTCTTGTA+AGG	+	chr2.1:9448117-9448136	None:intergenic	30.0%
!	GAAAAAGAAGAGCACAAATA+AGG	-	chr2.1:9448125-9448144	MS.gene54825:CDS	30.0%
!!!	TTCTCTTTTGAGATTCATAG+CGG	+	chr2.1:9447750-9447769	None:intergenic	30.0%
	AAGAAATTCCAACACATGGT+TGG	+	chr2.1:9447842-9447861	None:intergenic	35.0%
	ACAACCTTGTAAGTGAACTT+TGG	+	chr2.1:9447890-9447909	None:intergenic	35.0%
	ACCATGTGTTGGAATTTCTT+AGG	-	chr2.1:9447842-9447861	MS.gene54825:CDS	35.0%
	ACCTAAGAAATTCCAACACA+TGG	+	chr2.1:9447846-9447865	None:intergenic	35.0%
	CGTTTATCTTAGAGAGGTTA+GGG	-	chr2.1:9448065-9448084	MS.gene54825:CDS	35.0%
	TATTCGCGTTTATCTTAGAG+AGG	-	chr2.1:9448059-9448078	MS.gene54825:CDS	35.0%
!	AGAAGAGATTGGAACACTTT+TGG	-	chr2.1:9447766-9447785	MS.gene54825:CDS	35.0%
	AGTGGAAAGGATTTAGCTGA+AGG	-	chr2.1:9447691-9447710	MS.gene54825:CDS	40.0%
	ATGCTCTTATTGGGAGGTTA+AGG	-	chr2.1:9447980-9447999	MS.gene54825:CDS	40.0%
	ATGTCCACTTAGACAAGCTT+GGG	-	chr2.1:9447948-9447967	MS.gene54825:CDS	40.0%
	ATTGCAGTGAGAAAGTGGAA+CGG	+	chr2.1:9447818-9447837	None:intergenic	40.0%
	GAAGCCTTGATGCTCTTATT+GGG	-	chr2.1:9447971-9447990	MS.gene54825:CDS	40.0%
	GCGTTTATCTTAGAGAGGTT+AGG	-	chr2.1:9448064-9448083	MS.gene54825:CDS	40.0%
	TGCTCTTATTGGGAGGTTAA+GGG	-	chr2.1:9447981-9448000	MS.gene54825:CDS	40.0%
	TGTCTAAGTGGACATGTACA+AGG	+	chr2.1:9447943-9447962	None:intergenic	40.0%
	TTGGAATTGCAGTGAGAAAG+TGG	+	chr2.1:9447823-9447842	None:intergenic	40.0%
	AGGGCTGCATATGAGGAAAA+CGG	-	chr2.1:9448000-9448019	MS.gene54825:CDS	45.0%
	CATGTCCACTTAGACAAGCT+TGG	-	chr2.1:9447947-9447966	MS.gene54825:CDS	45.0%
	CTGCAATTCCAACCATGTGT+TGG	-	chr2.1:9447831-9447850	MS.gene54825:CDS	45.0%
	GGAAAGGATTTAGCTGAAGG+AGG	-	chr2.1:9447694-9447713	MS.gene54825:CDS	45.0%
	GGAAGCCTTGATGCTCTTAT+TGG	-	chr2.1:9447970-9447989	MS.gene54825:CDS	45.0%
	TGTCCACTTAGACAAGCTTG+GGG	-	chr2.1:9447949-9447968	MS.gene54825:CDS	45.0%
	TTGCAGTGAGAAAGTGGAAC+GGG	+	chr2.1:9447817-9447836	None:intergenic	45.0%
!!	ACCTCCCAATAAGAGCATCA+AGG	+	chr2.1:9447978-9447997	None:intergenic	45.0%
	AAAGTGGAACGGGTGGTCTA+AGG	+	chr2.1:9447807-9447826	None:intergenic	50.0%
	AGTGGACATGTACAAGGTGC+TGG	+	chr2.1:9447937-9447956	None:intergenic	50.0%
	CTTCCCCAAGCTTGTCTAAG+TGG	+	chr2.1:9447955-9447974	None:intergenic	50.0%
	GAGACTAATCCTTTCGCGAG+CGG	-	chr2.1:9448033-9448052	MS.gene54825:CDS	50.0%
	GAGGTTAAGGGCTGCATATG+AGG	-	chr2.1:9447993-9448012	MS.gene54825:CDS	50.0%
	GAGTGTCAAGCTAAGGCTAG+GGG	-	chr2.1:9448087-9448106	MS.gene54825:CDS	50.0%
	GCCTTGATGCTCTTATTGGG+AGG	-	chr2.1:9447974-9447993	MS.gene54825:CDS	50.0%
	GCTAAGTGTGATTCCTTGCG+AGG	+	chr2.1:9447728-9447747	None:intergenic	50.0%
	GCTGCATATGAGGAAAACGG+TGG	-	chr2.1:9448003-9448022	MS.gene54825:CDS	50.0%
	TGAGGAAAACGGTGGAACTC+CGG	-	chr2.1:9448011-9448030	MS.gene54825:CDS	50.0%
!	GGAGTGTCAAGCTAAGGCTA+GGG	-	chr2.1:9448086-9448105	MS.gene54825:CDS	50.0%
!!	CTCGCGAAAGGATTAGTCTC+CGG	+	chr2.1:9448033-9448052	None:intergenic	50.0%
!!	GGTTAGGGAGTGTCAAGCTA+AGG	-	chr2.1:9448080-9448099	MS.gene54825:CDS	50.0%
	CAGTGAGAAAGTGGAACGGG+TGG	+	chr2.1:9447814-9447833	None:intergenic	55.0%
	GGAGGATCATCAACCTCGCA+AGG	-	chr2.1:9447712-9447731	MS.gene54825:CDS	55.0%
!!	GGACATGTACAAGGTGCTGG+TGG	+	chr2.1:9447934-9447953	None:intergenic	55.0%
!!	GGGAGTGTCAAGCTAAGGCT+AGG	-	chr2.1:9448085-9448104	MS.gene54825:CDS	55.0%
	CGAATAGCACCGCTCGCGAA+AGG	+	chr2.1:9448045-9448064	None:intergenic	60.0%
!!	GTACAAGGTGCTGGTGGCTC+TGG	+	chr2.1:9447928-9447947	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	9447682	9448185	9447682	ID=MS.gene54825
chr2.1	mRNA	9447682	9448185	9447682	ID=MS.gene54825.t1;Parent=MS.gene54825
chr2.1	exon	9447682	9448185	9447682	ID=MS.gene54825.t1.exon1;Parent=MS.gene54825.t1
chr2.1	CDS	9447682	9448185	9447682	ID=cds.MS.gene54825.t1;Parent=MS.gene54825.t1

Gene Sequence

>MS.gene54825

ATGTCTTCAAGTGGAAAGGATTTAGCTGAAGGAGGATCATCAACCTCGCAAGGAATCACACTTAGCCGCTATGAATCTCAAAAGAGAAGAGATTGGAACACTTTTGGTCAATACTTAAACAACCTTAGACCACCCGTTCCACTTTCTCACTGCAATTCCAACCATGTGTTGGAATTTCTTAGGTATTTAGATCAATTTGGAAAAACCAAAGTTCACTTACAAGGTTGTATGTTTTATGGACAGCCAGAGCCACCAGCACCTTGTACATGTCCACTTAGACAAGCTTGGGGAAGCCTTGATGCTCTTATTGGGAGGTTAAGGGCTGCATATGAGGAAAACGGTGGAACTCCGGAGACTAATCCTTTCGCGAGCGGTGCTATTCGCGTTTATCTTAGAGAGGTTAGGGAGTGTCAAGCTAAGGCTAGGGGTATCCCTTACAAGAAGAAAAAGAAGAGCACAAATAAGGGAAATGATGAATCAAGCTCAACCATGCACTTTTCTTGA

Protein sequence

>MS.gene54825.t1

MSSSGKDLAEGGSSTSQGITLSRYESQKRRDWNTFGQYLNNLRPPVPLSHCNSNHVLEFLRYLDQFGKTKVHLQGCMFYGQPEPPAPCTCPLRQAWGSLDALIGRLRAAYEENGGTPETNPFASGAIRVYLREVRECQAKARGIPYKKKKKSTNKGNDESSSTMHFS