Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55054.t1 | XP_004492941.1 | 98.3 | 115 | 2 | 0 | 1 | 115 | 99 | 213 | 4.10E-55 | 223.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55054.t1 | A0A1S2XQH7 | 98.3 | 115 | 2 | 0 | 1 | 115 | 99 | 213 | 3.0e-55 | 223.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050945 | MS.gene55054 | 0.800333 | 1.51E-48 | -1.69E-46 |
| MS.gene051234 | MS.gene55054 | 0.800614 | 1.33E-48 | -1.69E-46 |
| MS.gene061100 | MS.gene55054 | 0.801715 | 7.90E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55054.t1 | MTR_7g082140 | 98.261 | 115 | 2 | 0 | 1 | 115 | 99 | 213 | 1.49e-77 | 229 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55054.t1 | AT1G67060 | 84.348 | 115 | 17 | 1 | 1 | 115 | 34 | 147 | 1.07e-62 | 188 |
| MS.gene55054.t1 | AT1G67060 | 84.348 | 115 | 17 | 1 | 1 | 115 | 34 | 147 | 1.07e-62 | 188 |
| MS.gene55054.t1 | AT1G67060 | 84.348 | 115 | 17 | 1 | 1 | 115 | 101 | 214 | 1.41e-61 | 188 |
Find 14 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTGCCCTTGTAATGGTTT+TGG | 0.181287 | 7.2:+24901820 | MS.gene55054:CDS |
| GAATGACATAGCGAAGGATA+TGG | 0.348587 | 7.2:-24901466 | None:intergenic |
| TGGTTGCTTTCTTGCTGCTC+TGG | 0.389334 | 7.2:+24901139 | MS.gene55054:CDS |
| TCTGGTTATTGTGCTCTTTG+TGG | 0.424444 | 7.2:+24901157 | MS.gene55054:CDS |
| GTCTGCCCTTGCCCTTGTAA+TGG | 0.430059 | 7.2:+24901814 | MS.gene55054:CDS |
| CGCTATGTCATTCTCTTCAT+CGG | 0.432806 | 7.2:+24901476 | MS.gene55054:CDS |
| CTTACTGCAAGGATTGCTGC+TGG | 0.492007 | 7.2:+24901119 | MS.gene55054:CDS |
| CCCCACCCAAAACCATTACA+AGG | 0.500731 | 7.2:-24901826 | None:intergenic |
| CTACAAATATTCTTACTGCA+AGG | 0.548085 | 7.2:+24901108 | MS.gene55054:CDS |
| ACACTGCGGGGACTCTGTAT+CGG | 0.572546 | 7.2:+24901303 | MS.gene55054:CDS |
| CCAAAACCATTACAAGGGCA+AGG | 0.586840 | 7.2:-24901820 | None:intergenic |
| TGAAGAGAATGACATAGCGA+AGG | 0.629946 | 7.2:-24901472 | None:intergenic |
| CAAAACCATTACAAGGGCAA+GGG | 0.650178 | 7.2:-24901819 | None:intergenic |
| CCCACCCAAAACCATTACAA+GGG | 0.672104 | 7.2:-24901825 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATGAAACGTATAAAAAG+TGG | - | chr7.2:24901669-24901688 | None:intergenic | 20.0% |
| !! | ATTCAATCAAATGATATAAG+TGG | - | chr7.2:24901718-24901737 | None:intergenic | 20.0% |
| !!! | CTAATTATGATTTGATTTTC+AGG | + | chr7.2:24901602-24901621 | MS.gene55054:intron | 20.0% |
| !!! | TACAATCTTCTGAAATAAAA+AGG | + | chr7.2:24901336-24901355 | MS.gene55054:intron | 20.0% |
| !!! | TATACGTTTCATTTTCAATT+TGG | + | chr7.2:24901674-24901693 | MS.gene55054:intron | 20.0% |
| !!! | TCATTTGATTGAATTTTGTA+TGG | + | chr7.2:24901724-24901743 | MS.gene55054:intron | 20.0% |
| ! | TTCTTGATCAATGAATATCA+GGG | + | chr7.2:24901393-24901412 | MS.gene55054:intron | 25.0% |
| !!! | AACAGTTTGTTTTCAGTTTA+TGG | + | chr7.2:24901632-24901651 | MS.gene55054:CDS | 25.0% |
| !!! | TGTCATTTCTTTGAAATGTA+TGG | + | chr7.2:24901561-24901580 | MS.gene55054:intron | 25.0% |
| !!! | TTGTTTTTATTGCTGTGATT+TGG | + | chr7.2:24901420-24901439 | MS.gene55054:CDS | 25.0% |
| AAAGGAAAGACGATATCATT+AGG | + | chr7.2:24901354-24901373 | MS.gene55054:intron | 30.0% | |
| AAGGAAAGACGATATCATTA+GGG | + | chr7.2:24901355-24901374 | MS.gene55054:intron | 30.0% | |
| CTACAAATATTCTTACTGCA+AGG | + | chr7.2:24901108-24901127 | MS.gene55054:CDS | 30.0% | |
| CTTCTTGATCAATGAATATC+AGG | + | chr7.2:24901392-24901411 | MS.gene55054:intron | 30.0% | |
| AAATGACACATATACCAACC+AGG | - | chr7.2:24901549-24901568 | None:intergenic | 35.0% | |
| AATGACACATATACCAACCA+GGG | - | chr7.2:24901548-24901567 | None:intergenic | 35.0% | |
| ACATACCCTTATCCAAAAAG+TGG | + | chr7.2:24901280-24901299 | MS.gene55054:intron | 35.0% | |
| ATGTTAGCGAGACAGAAAAA+GGG | - | chr7.2:24901264-24901283 | None:intergenic | 35.0% | |
| TATGTTAGCGAGACAGAAAA+AGG | - | chr7.2:24901265-24901284 | None:intergenic | 35.0% | |
| TATTTGAAGAACACGTGAAG+AGG | - | chr7.2:24901513-24901532 | None:intergenic | 35.0% | |
| !! | AAATATTTTGAACCTGTCCC+TGG | + | chr7.2:24901528-24901547 | MS.gene55054:intron | 35.0% |
| !!! | AGTGTCCACTTTTTGGATAA+GGG | - | chr7.2:24901288-24901307 | None:intergenic | 35.0% |
| CAAAACCATTACAAGGGCAA+GGG | - | chr7.2:24901822-24901841 | None:intergenic | 40.0% | |
| CGCTATGTCATTCTCTTCAT+CGG | + | chr7.2:24901476-24901495 | MS.gene55054:CDS | 40.0% | |
| GAATGACATAGCGAAGGATA+TGG | - | chr7.2:24901469-24901488 | None:intergenic | 40.0% | |
| TATCCAAAAAGTGGACACTG+CGG | + | chr7.2:24901289-24901308 | MS.gene55054:intron | 40.0% | |
| TCTGGTTATTGTGCTCTTTG+TGG | + | chr7.2:24901157-24901176 | MS.gene55054:CDS | 40.0% | |
| TGAAGAGAATGACATAGCGA+AGG | - | chr7.2:24901475-24901494 | None:intergenic | 40.0% | |
| ! | CTTGCCCTTGTAATGGTTTT+GGG | + | chr7.2:24901821-24901840 | MS.gene55054:CDS | 40.0% |
| !!! | CAGTGTCCACTTTTTGGATA+AGG | - | chr7.2:24901289-24901308 | None:intergenic | 40.0% |
| ATCCAAAAAGTGGACACTGC+GGG | + | chr7.2:24901290-24901309 | MS.gene55054:intron | 45.0% | |
| CCAAAACCATTACAAGGGCA+AGG | - | chr7.2:24901823-24901842 | None:intergenic | 45.0% | |
| CCCACCCAAAACCATTACAA+GGG | - | chr7.2:24901828-24901847 | None:intergenic | 45.0% | |
| ! | ATTTTGAACCTGTCCCTGGT+TGG | + | chr7.2:24901532-24901551 | MS.gene55054:intron | 45.0% |
| ! | CCTTGCCCTTGTAATGGTTT+TGG | + | chr7.2:24901820-24901839 | MS.gene55054:CDS | 45.0% |
| !! | GTTTTGGGTGGGGAGTTATT+TGG | + | chr7.2:24901836-24901855 | MS.gene55054:CDS | 45.0% |
| !!! | CCCTTGTAATGGTTTTGGGT+GGG | + | chr7.2:24901825-24901844 | MS.gene55054:CDS | 45.0% |
| !!! | CCTTGTAATGGTTTTGGGTG+GGG | + | chr7.2:24901826-24901845 | MS.gene55054:CDS | 45.0% |
| CACATATACCAACCAGGGAC+AGG | - | chr7.2:24901543-24901562 | None:intergenic | 50.0% | |
| CCCCACCCAAAACCATTACA+AGG | - | chr7.2:24901829-24901848 | None:intergenic | 50.0% | |
| CTTACTGCAAGGATTGCTGC+TGG | + | chr7.2:24901119-24901138 | MS.gene55054:CDS | 50.0% | |
| GAAGAACACGTGAAGAGGAC+AGG | - | chr7.2:24901508-24901527 | None:intergenic | 50.0% | |
| TCCAAAAAGTGGACACTGCG+GGG | + | chr7.2:24901291-24901310 | MS.gene55054:intron | 50.0% | |
| ! | TGGTTGCTTTCTTGCTGCTC+TGG | + | chr7.2:24901139-24901158 | MS.gene55054:CDS | 50.0% |
| !!! | GCCCTTGTAATGGTTTTGGG+TGG | + | chr7.2:24901824-24901843 | MS.gene55054:CDS | 50.0% |
| ACACTGCGGGGACTCTGTAT+CGG | + | chr7.2:24901303-24901322 | MS.gene55054:CDS | 55.0% | |
| GTCTGCCCTTGCCCTTGTAA+TGG | + | chr7.2:24901814-24901833 | MS.gene55054:CDS | 55.0% | |
| !! | TCCCCGCAGTGTCCACTTTT+TGG | - | chr7.2:24901295-24901314 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 24901089 | 24901861 | 24901089 | ID=MS.gene55054 |
| chr7.2 | mRNA | 24901089 | 24901861 | 24901089 | ID=MS.gene55054.t1;Parent=MS.gene55054 |
| chr7.2 | exon | 24901089 | 24901187 | 24901089 | ID=MS.gene55054.t1.exon1;Parent=MS.gene55054.t1 |
| chr7.2 | CDS | 24901089 | 24901187 | 24901089 | ID=cds.MS.gene55054.t1;Parent=MS.gene55054.t1 |
| chr7.2 | exon | 24901300 | 24901324 | 24901300 | ID=MS.gene55054.t1.exon2;Parent=MS.gene55054.t1 |
| chr7.2 | CDS | 24901300 | 24901324 | 24901300 | ID=cds.MS.gene55054.t1;Parent=MS.gene55054.t1 |
| chr7.2 | exon | 24901414 | 24901497 | 24901414 | ID=MS.gene55054.t1.exon3;Parent=MS.gene55054.t1 |
| chr7.2 | CDS | 24901414 | 24901497 | 24901414 | ID=cds.MS.gene55054.t1;Parent=MS.gene55054.t1 |
| chr7.2 | exon | 24901624 | 24901653 | 24901624 | ID=MS.gene55054.t1.exon4;Parent=MS.gene55054.t1 |
| chr7.2 | CDS | 24901624 | 24901653 | 24901624 | ID=cds.MS.gene55054.t1;Parent=MS.gene55054.t1 |
| chr7.2 | exon | 24901752 | 24901861 | 24901752 | ID=MS.gene55054.t1.exon5;Parent=MS.gene55054.t1 |
| chr7.2 | CDS | 24901752 | 24901861 | 24901752 | ID=cds.MS.gene55054.t1;Parent=MS.gene55054.t1 |
| Gene Sequence |
| Protein sequence |
