AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene55676


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55676.t1 MTR_3g063310 97.619 252 6 0 1 252 1 252 0.0 509
MS.gene55676.t1 MTR_5g076170 61.597 263 89 3 2 252 1 263 1.52e-97 286
MS.gene55676.t1 MTR_1g077560 48.819 254 123 5 2 252 1 250 2.13e-68 211
MS.gene55676.t1 MTR_5g087760 35.648 216 132 4 40 252 43 254 5.85e-36 128
MS.gene55676.t1 MTR_3g078800 38.251 183 106 3 72 250 73 252 1.05e-33 123
MS.gene55676.t1 MTR_2g012550 33.824 204 120 6 55 252 70 264 1.49e-28 109
MS.gene55676.t1 MTR_2g011350 33.113 151 94 3 80 227 35 181 5.96e-23 93.2
MS.gene55676.t1 MTR_3g104460 30.846 201 126 6 61 252 57 253 4.38e-22 92.0
MS.gene55676.t1 MTR_2g100060 30.726 179 115 5 81 252 77 253 1.39e-21 90.9
MS.gene55676.t1 MTR_3g078820 30.570 193 122 4 46 233 4 189 2.16e-21 89.4
MS.gene55676.t1 MTR_3g074150 26.471 238 160 5 15 247 15 242 3.73e-20 87.0
MS.gene55676.t1 MTR_6g071410 34.559 136 80 4 62 194 2 131 4.79e-19 83.2
MS.gene55676.t1 MTR_6g070890 34.559 136 80 4 62 194 2 131 4.79e-19 83.2
MS.gene55676.t1 MTR_1g057400 29.412 187 123 5 51 230 3 187 2.85e-18 80.9
MS.gene55676.t1 MTR_2g086790 27.320 194 128 2 70 250 20 213 3.19e-18 80.9
MS.gene55676.t1 MTR_6g471090 38.462 130 69 6 66 194 10 129 4.85e-18 80.1
MS.gene55676.t1 MTR_1g057270 31.818 176 104 5 87 250 75 246 2.85e-17 79.0
MS.gene55676.t1 MTR_6g070900 31.613 155 98 6 62 211 2 153 3.45e-17 78.2
MS.gene55676.t1 MTR_6g071400 31.613 155 98 6 62 211 2 153 3.45e-17 78.2
MS.gene55676.t1 MTR_4g119428 31.892 185 104 5 59 230 41 216 5.04e-17 78.2
MS.gene55676.t1 MTR_3g078810 33.333 126 73 3 120 239 14 134 2.27e-16 74.3
MS.gene55676.t1 MTR_7g106050 28.497 193 131 4 53 244 9 195 2.65e-16 75.5
MS.gene55676.t1 MTR_8g090320 26.344 186 109 5 61 239 83 247 7.27e-16 75.1
MS.gene55676.t1 MTR_4g051880 32.544 169 103 4 92 250 44 211 2.38e-15 73.2
MS.gene55676.t1 MTR_7g106000 26.941 219 139 8 37 246 16 222 8.20e-15 71.6
MS.gene55676.t1 MTR_2g090225 38.095 147 78 5 93 233 62 201 9.46e-15 71.6
MS.gene55676.t1 MTR_1g057420 27.273 165 116 4 88 250 39 201 2.99e-12 64.3
MS.gene55676.t1 MTR_6g471080 32.895 152 86 6 66 211 7 148 7.45e-12 63.2
MS.gene55676.t1 MTR_7g105870 33.129 163 106 3 70 230 44 205 1.15e-11 63.2
MS.gene55676.t1 MTR_8g016370 23.346 257 152 8 2 252 1 218 1.38e-11 62.8
MS.gene55676.t1 MTR_3g092790 30.882 136 91 3 92 226 49 182 4.07e-11 61.2
MS.gene55676.t1 MTR_7g106120 27.778 126 84 3 53 172 2 126 6.83e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55676.t1 AT1G65690 58.039 255 100 3 2 252 1 252 1.44e-96 283
MS.gene55676.t1 AT5G36970 51.793 251 118 1 2 252 1 248 2.06e-85 254
MS.gene55676.t1 AT1G54540 43.651 252 128 4 2 252 1 239 2.15e-58 186
MS.gene55676.t1 AT2G27080 34.615 182 113 3 59 239 72 248 2.68e-34 124
MS.gene55676.t1 AT2G27080 34.615 182 113 3 59 239 72 248 2.68e-34 124
MS.gene55676.t1 AT3G11650 29.167 216 133 6 36 237 14 223 1.20e-23 95.9
MS.gene55676.t1 AT2G35980 35.233 193 110 6 40 227 18 200 2.11e-23 95.1
MS.gene55676.t1 AT5G21130 25.641 195 145 0 56 250 86 280 3.62e-22 92.8
MS.gene55676.t1 AT5G06320 30.769 208 124 7 36 230 10 210 1.70e-21 90.1
MS.gene55676.t1 AT3G52470 26.804 194 135 5 59 250 12 200 9.08e-17 76.6
MS.gene55676.t1 AT2G35460 25.000 184 133 4 71 250 56 238 4.00e-16 75.5
MS.gene55676.t1 AT3G44220 29.268 164 116 0 71 234 22 185 4.58e-16 74.7
MS.gene55676.t1 AT4G09590 29.944 177 120 4 76 250 29 203 2.01e-15 73.2
MS.gene55676.t1 AT5G22870 26.012 173 127 1 65 237 25 196 2.76e-15 72.8
MS.gene55676.t1 AT2G22180 25.463 216 138 5 40 251 94 290 5.05e-15 73.6
MS.gene55676.t1 AT1G17620 29.707 239 143 10 2 226 1 228 5.62e-15 72.8
MS.gene55676.t1 AT2G35970 29.944 177 120 4 76 250 29 203 7.97e-15 71.6
MS.gene55676.t1 AT3G11660 25.275 182 131 4 70 248 20 199 1.57e-13 67.8
MS.gene55676.t1 AT2G35960 25.444 169 124 2 83 250 35 202 7.43e-13 66.2
MS.gene55676.t1 AT5G53730 27.879 165 110 3 74 236 38 195 8.85e-13 65.9
MS.gene55676.t1 AT5G22200 30.216 139 97 0 88 226 43 181 2.51e-12 64.7
MS.gene55676.t1 AT2G46300 30.208 192 125 5 44 227 37 227 2.18e-11 62.8

Find 59 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGTCTGTCTACGGAGTATT+TGG 0.188778 3.3:-57167106 None:intergenic
TATATTCCAATCTTCTTGTT+AGG 0.211306 3.3:-57167204 None:intergenic
CTTTATACCAAGCACTTATA+TGG 0.275071 3.3:-57167242 None:intergenic
TCCTGTGTTTGACCTGTTAA+AGG 0.282421 3.3:-57167336 None:intergenic
TAATATACCACTTAATATTA+AGG 0.282779 3.3:+57167407 MS.gene55676:CDS
GTCTCTGCCGAAATTCTATC+AGG 0.301530 3.3:+57167284 MS.gene55676:CDS
ACGGAGTATTTGGGAAGTTT+TGG 0.306221 3.3:-57167096 None:intergenic
TCGTGTTAGGTGCAGCCTTC+AGG 0.318841 3.3:+57167485 MS.gene55676:CDS
CGATGACCCTGATAGAATTT+CGG 0.348072 3.3:-57167291 None:intergenic
AATTCACCCTGCTAGTGATT+TGG 0.348837 3.3:+57166840 MS.gene55676:CDS
GTGCTTAATCTACCTTTAAC+AGG 0.355672 3.3:+57167324 MS.gene55676:CDS
TTAGAGTCAAGTTTCGTGTT+AGG 0.366756 3.3:+57167472 MS.gene55676:CDS
TTGCAATTGGCATCACTGTT+GGG 0.370285 3.3:+57167052 MS.gene55676:CDS
CTGATTCTTATAATTGCAAT+TGG 0.380687 3.3:+57167039 MS.gene55676:CDS
TGCAGTTACTGTTACATTGA+AGG 0.386255 3.3:-57167175 None:intergenic
ATATTCCAATCTTCTTGTTA+GGG 0.397601 3.3:-57167203 None:intergenic
AATTATAAGAATCAGCAATA+TGG 0.406684 3.3:-57167031 None:intergenic
AAACAAAGGAACTGTAGGTT+TGG 0.417723 3.3:-57166872 None:intergenic
TGATTAACCTTAATATTAAG+TGG 0.432715 3.3:-57167414 None:intergenic
AGAAACCCTAACAAGAAGAT+TGG 0.436387 3.3:+57167198 MS.gene55676:CDS
AGTGCTTGGTATAAAGAAAC+AGG 0.436753 3.3:+57167249 MS.gene55676:CDS
CTCACTGGAAAGTGGCGTTT+CGG 0.437034 3.3:-57166946 None:intergenic
CAGATAGTAAATGTAAGTTC+AGG 0.455434 3.3:+57167544 MS.gene55676:CDS
GGTGGATGAGTGTGCCTCAC+TGG 0.457753 3.3:-57166961 None:intergenic
GGAAAGTGGCGTTTCGGAAG+AGG 0.477952 3.3:-57166940 None:intergenic
AGTCTGTCTACGGAGTATTT+GGG 0.485802 3.3:-57167105 None:intergenic
ATTGCAATTGGCATCACTGT+TGG 0.490412 3.3:+57167051 MS.gene55676:CDS
CGAGGAAACAAAGGAACTGT+AGG 0.498817 3.3:-57166877 None:intergenic
TCACCCTGCTAGTGATTTGG+AGG 0.499157 3.3:+57166843 MS.gene55676:CDS
AGAAAAGAAGTTGCTGCTGC+AGG 0.502944 3.3:+57166989 MS.gene55676:CDS
CAGCAGCAGCTACAAGAAGC+AGG 0.503374 3.3:+57167384 MS.gene55676:CDS
GGTCAAACACAGGATGCAAC+TGG 0.504890 3.3:+57167345 MS.gene55676:CDS
TGTGCCTCCAAATCACTAGC+AGG 0.513508 3.3:-57166847 None:intergenic
ATTGGAATATACTATGTAAG+TGG 0.516967 3.3:+57167216 MS.gene55676:CDS
CAGAATGTGAGGGTTAAACT+TGG 0.517481 3.3:+57167435 MS.gene55676:CDS
AGCCTTCAGGTGGATAGTCT+TGG 0.518857 3.3:+57167498 MS.gene55676:CDS
ATGTGAAGGACTGCGTCGAT+CGG 0.529155 3.3:-57166914 None:intergenic
GTAAATGTAAGTTCAGGCTC+AGG 0.540224 3.3:+57167550 MS.gene55676:CDS
AAGAAACAGGACTGTGTGAA+GGG 0.543431 3.3:+57167262 MS.gene55676:CDS
TGCAATTGGCATCACTGTTG+GGG 0.544430 3.3:+57167053 MS.gene55676:CDS
CGAAATTCTATCAGGGTCAT+CGG 0.547495 3.3:+57167292 MS.gene55676:CDS
TGTTATTCTGAGTCTGTCTA+CGG 0.555736 3.3:-57167115 None:intergenic
TTAAGGTTAATCAGAATGTG+AGG 0.560781 3.3:+57167424 MS.gene55676:CDS
GTGGAAGCCATATAAGTGCT+TGG 0.564620 3.3:+57167235 MS.gene55676:CDS
TGTTAGGTGCAGCCTTCAGG+TGG 0.567547 3.3:+57167488 MS.gene55676:CDS
TTCGGAAGAGGTGGATGTGA+AGG 0.571122 3.3:-57166928 None:intergenic
AAAGAAACAGGACTGTGTGA+AGG 0.583033 3.3:+57167261 MS.gene55676:CDS
GAAGTGTTTCGAGGAAACAA+AGG 0.599067 3.3:-57166886 None:intergenic
AAGAATCAGCAATATGGTGA+AGG 0.607184 3.3:-57167025 None:intergenic
AAGTGGCGTTTCGGAAGAGG+TGG 0.610030 3.3:-57166937 None:intergenic
GAGTGTGCCTCACTGGAAAG+TGG 0.615492 3.3:-57166954 None:intergenic
TAAGGTTAATCAGAATGTGA+GGG 0.620913 3.3:+57167425 MS.gene55676:CDS
AACAAAGAGACTGTTGTCGT+CGG 0.621599 3.3:-57167151 None:intergenic
TGTTAAAGGTAGATTAAGCA+CGG 0.622717 3.3:-57167322 None:intergenic
CACCAAGACTATCCACCTGA+AGG 0.627283 3.3:-57167500 None:intergenic
ACCTTTAACAGGTCAAACAC+AGG 0.638297 3.3:+57167335 MS.gene55676:CDS
TCTCTGCCGAAATTCTATCA+GGG 0.641999 3.3:+57167285 MS.gene55676:CDS
GTGCCTCCAAATCACTAGCA+GGG 0.648239 3.3:-57166846 None:intergenic
CGAAACGCCACTTTCCAGTG+AGG 0.695288 3.3:+57166947 MS.gene55676:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATATACCACTTAATATTA+AGG + chr3.3:57167407-57167426 MS.gene55676:CDS 15.0%
!! AATTATAAGAATCAGCAATA+TGG - chr3.3:57167034-57167053 None:intergenic 20.0%
!! TGATTAACCTTAATATTAAG+TGG - chr3.3:57167417-57167436 None:intergenic 20.0%
! ATATTCCAATCTTCTTGTTA+GGG - chr3.3:57167206-57167225 None:intergenic 25.0%
! ATTGGAATATACTATGTAAG+TGG + chr3.3:57167216-57167235 MS.gene55676:CDS 25.0%
! CTGATTCTTATAATTGCAAT+TGG + chr3.3:57167039-57167058 MS.gene55676:CDS 25.0%
! TATATTCCAATCTTCTTGTT+AGG - chr3.3:57167207-57167226 None:intergenic 25.0%
CAGATAGTAAATGTAAGTTC+AGG + chr3.3:57167544-57167563 MS.gene55676:CDS 30.0%
CTTTATACCAAGCACTTATA+TGG - chr3.3:57167245-57167264 None:intergenic 30.0%
TAAGGTTAATCAGAATGTGA+GGG + chr3.3:57167425-57167444 MS.gene55676:CDS 30.0%
TGTTAAAGGTAGATTAAGCA+CGG - chr3.3:57167325-57167344 None:intergenic 30.0%
TTAAGGTTAATCAGAATGTG+AGG + chr3.3:57167424-57167443 MS.gene55676:CDS 30.0%
AAACAAAGGAACTGTAGGTT+TGG - chr3.3:57166875-57166894 None:intergenic 35.0%
AAGAATCAGCAATATGGTGA+AGG - chr3.3:57167028-57167047 None:intergenic 35.0%
AGAAACCCTAACAAGAAGAT+TGG + chr3.3:57167198-57167217 MS.gene55676:CDS 35.0%
AGTGCTTGGTATAAAGAAAC+AGG + chr3.3:57167249-57167268 MS.gene55676:CDS 35.0%
GTGCTTAATCTACCTTTAAC+AGG + chr3.3:57167324-57167343 MS.gene55676:CDS 35.0%
TGCAGTTACTGTTACATTGA+AGG - chr3.3:57167178-57167197 None:intergenic 35.0%
TGTTATTCTGAGTCTGTCTA+CGG - chr3.3:57167118-57167137 None:intergenic 35.0%
TTAGAGTCAAGTTTCGTGTT+AGG + chr3.3:57167472-57167491 MS.gene55676:CDS 35.0%
! CAACTTCTTTTCTTCTTTGG+TGG - chr3.3:57166982-57167001 None:intergenic 35.0%
! CAGCAACTTCTTTTCTTCTT+TGG - chr3.3:57166985-57167004 None:intergenic 35.0%
AAAGAAACAGGACTGTGTGA+AGG + chr3.3:57167261-57167280 MS.gene55676:CDS 40.0%
AACAAAGAGACTGTTGTCGT+CGG - chr3.3:57167154-57167173 None:intergenic 40.0%
AAGAAACAGGACTGTGTGAA+GGG + chr3.3:57167262-57167281 MS.gene55676:CDS 40.0%
AATTCACCCTGCTAGTGATT+TGG + chr3.3:57166840-57166859 MS.gene55676:CDS 40.0%
ACCTTTAACAGGTCAAACAC+AGG + chr3.3:57167335-57167354 MS.gene55676:CDS 40.0%
ATTGCAATTGGCATCACTGT+TGG + chr3.3:57167051-57167070 MS.gene55676:CDS 40.0%
CAGAATGTGAGGGTTAAACT+TGG + chr3.3:57167435-57167454 MS.gene55676:CDS 40.0%
CGAAATTCTATCAGGGTCAT+CGG + chr3.3:57167292-57167311 MS.gene55676:CDS 40.0%
CGATGACCCTGATAGAATTT+CGG - chr3.3:57167294-57167313 None:intergenic 40.0%
GTAAATGTAAGTTCAGGCTC+AGG + chr3.3:57167550-57167569 MS.gene55676:CDS 40.0%
TCCTGTGTTTGACCTGTTAA+AGG - chr3.3:57167339-57167358 None:intergenic 40.0%
TCTCTGCCGAAATTCTATCA+GGG + chr3.3:57167285-57167304 MS.gene55676:CDS 40.0%
TTGCAATTGGCATCACTGTT+GGG + chr3.3:57167052-57167071 MS.gene55676:CDS 40.0%
! AGTCTGTCTACGGAGTATTT+GGG - chr3.3:57167108-57167127 None:intergenic 40.0%
! GAAGTGTTTCGAGGAAACAA+AGG - chr3.3:57166889-57166908 None:intergenic 40.0%
!! ACGGAGTATTTGGGAAGTTT+TGG - chr3.3:57167099-57167118 None:intergenic 40.0%
!! GAAGTTTTGGCCTAAAAGCA+AGG - chr3.3:57167086-57167105 None:intergenic 40.0%
!!! TCGGATTTTGAAGTGTTTCG+AGG - chr3.3:57166898-57166917 None:intergenic 40.0%
AGAAAAGAAGTTGCTGCTGC+AGG + chr3.3:57166989-57167008 MS.gene55676:CDS 45.0%
CGAGGAAACAAAGGAACTGT+AGG - chr3.3:57166880-57166899 None:intergenic 45.0%
GTCTCTGCCGAAATTCTATC+AGG + chr3.3:57167284-57167303 MS.gene55676:CDS 45.0%
GTGGAAGCCATATAAGTGCT+TGG + chr3.3:57167235-57167254 MS.gene55676:CDS 45.0%
TGCAATTGGCATCACTGTTG+GGG + chr3.3:57167053-57167072 MS.gene55676:CDS 45.0%
! GAGTCTGTCTACGGAGTATT+TGG - chr3.3:57167109-57167128 None:intergenic 45.0%
AGCCTTCAGGTGGATAGTCT+TGG + chr3.3:57167498-57167517 MS.gene55676:CDS 50.0%
CACCAAGACTATCCACCTGA+AGG - chr3.3:57167503-57167522 None:intergenic 50.0%
CTCACTGGAAAGTGGCGTTT+CGG - chr3.3:57166949-57166968 None:intergenic 50.0%
GGTCAAACACAGGATGCAAC+TGG + chr3.3:57167345-57167364 MS.gene55676:CDS 50.0%
GTGCCTCCAAATCACTAGCA+GGG - chr3.3:57166849-57166868 None:intergenic 50.0%
TGTGCCTCCAAATCACTAGC+AGG - chr3.3:57166850-57166869 None:intergenic 50.0%
TTCGGAAGAGGTGGATGTGA+AGG - chr3.3:57166931-57166950 None:intergenic 50.0%
! ATGTGAAGGACTGCGTCGAT+CGG - chr3.3:57166917-57166936 None:intergenic 50.0%
! TCACCCTGCTAGTGATTTGG+AGG + chr3.3:57166843-57166862 MS.gene55676:CDS 50.0%
!! GGGCACTCTACCTTGCTTTT+AGG + chr3.3:57167073-57167092 MS.gene55676:CDS 50.0%
AAGTGGCGTTTCGGAAGAGG+TGG - chr3.3:57166940-57166959 None:intergenic 55.0%
CAGCAGCAGCTACAAGAAGC+AGG + chr3.3:57167384-57167403 MS.gene55676:CDS 55.0%
GAGTGTGCCTCACTGGAAAG+TGG - chr3.3:57166957-57166976 None:intergenic 55.0%
GGAAAGTGGCGTTTCGGAAG+AGG - chr3.3:57166943-57166962 None:intergenic 55.0%
TCGTGTTAGGTGCAGCCTTC+AGG + chr3.3:57167485-57167504 MS.gene55676:CDS 55.0%
TGTTAGGTGCAGCCTTCAGG+TGG + chr3.3:57167488-57167507 MS.gene55676:CDS 55.0%
! CGAAACGCCACTTTCCAGTG+AGG + chr3.3:57166947-57166966 MS.gene55676:CDS 55.0%
GGTGGATGAGTGTGCCTCAC+TGG - chr3.3:57166964-57166983 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 57166820 57167578 57166820 ID=MS.gene55676
chr3.3 mRNA 57166820 57167578 57166820 ID=MS.gene55676.t1;Parent=MS.gene55676
chr3.3 exon 57166820 57167578 57166820 ID=MS.gene55676.t1.exon1;Parent=MS.gene55676.t1
chr3.3 CDS 57166820 57167578 57166820 ID=cds.MS.gene55676.t1;Parent=MS.gene55676.t1
Gene Sequence

>MS.gene55676

ATGATGACAGATCATCAGAGAATTCACCCTGCTAGTGATTTGGAGGCACAAACCAAACCTACAGTTCCTTTGTTTCCTCGAAACACTTCAAAATCCGATCGACGCAGTCCTTCACATCCACCTCTTCCGAAACGCCACTTTCCAGTGAGGCACACTCATCCACCAAAGAAGAAAAGAAGTTGCTGCTGCAGGTTCTTGTGTTGTACCTTCACCATATTGCTGATTCTTATAATTGCAATTGGCATCACTGTTGGGGCACTCTACCTTGCTTTTAGGCCAAAACTTCCCAAATACTCCGTAGACAGACTCAGAATAACACAGTTCAATCTTTCCGACGACAACAGTCTCTTTGTTACCTTCAATGTAACAGTAACTGCAAGAAACCCTAACAAGAAGATTGGAATATACTATGTAAGTGGAAGCCATATAAGTGCTTGGTATAAAGAAACAGGACTGTGTGAAGGGTCTCTGCCGAAATTCTATCAGGGTCATCGGAATACAACCGTGCTTAATCTACCTTTAACAGGTCAAACACAGGATGCAACTGGTTTATTTAATACGTTGCAGCAGCAGCTACAAGAAGCAGGTAATATACCACTTAATATTAAGGTTAATCAGAATGTGAGGGTTAAACTTGGTAAGTTGAAGATCTTTAGAGTCAAGTTTCGTGTTAGGTGCAGCCTTCAGGTGGATAGTCTTGGTGCTGATAATGATATTAGTATTTCAGATAGTAAATGTAAGTTCAGGCTCAGGCTATGA

Protein sequence

>MS.gene55676.t1

MMTDHQRIHPASDLEAQTKPTVPLFPRNTSKSDRRSPSHPPLPKRHFPVRHTHPPKKKRSCCCRFLCCTFTILLILIIAIGITVGALYLAFRPKLPKYSVDRLRITQFNLSDDNSLFVTFNVTVTARNPNKKIGIYYVSGSHISAWYKETGLCEGSLPKFYQGHRNTTVLNLPLTGQTQDATGLFNTLQQQLQEAGNIPLNIKVNQNVRVKLGKLKIFRVKFRVRCSLQVDSLGADNDISISDSKCKFRLRL