Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55884.t1 | XP_030488294.1 | 84.2 | 120 | 19 | 0 | 2 | 121 | 3 | 122 | 8.20E-54 | 219.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55884.t1 | A0A1S2YE81 | 84.2 | 120 | 19 | 0 | 2 | 121 | 3 | 122 | 7.7e-54 | 219.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050320 | MS.gene55884 | 0.80646 | 8.14E-50 | -1.69E-46 |
| MS.gene051748 | MS.gene55884 | 0.820736 | 5.86E-53 | -1.69E-46 |
| MS.gene051756 | MS.gene55884 | 0.830599 | 2.68E-55 | -1.69E-46 |
| MS.gene051903 | MS.gene55884 | 0.830633 | 2.63E-55 | -1.69E-46 |
| MS.gene057094 | MS.gene55884 | 0.805149 | 1.53E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55884.t1 | MTR_8g101880 | 98.347 | 121 | 2 | 0 | 1 | 121 | 1 | 121 | 2.34e-87 | 249 |
| MS.gene55884.t1 | MTR_8g101890 | 78.333 | 120 | 26 | 0 | 2 | 121 | 3 | 122 | 1.83e-70 | 207 |
| MS.gene55884.t1 | MTR_2g100380 | 61.667 | 120 | 46 | 0 | 2 | 121 | 98 | 217 | 8.90e-52 | 162 |
| MS.gene55884.t1 | MTR_5g062980 | 69.492 | 59 | 18 | 0 | 58 | 116 | 1 | 59 | 2.33e-26 | 95.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55884.t1 | AT4G26210 | 73.950 | 119 | 31 | 0 | 2 | 120 | 3 | 121 | 5.23e-66 | 196 |
| MS.gene55884.t1 | AT4G26210 | 73.950 | 119 | 31 | 0 | 2 | 120 | 3 | 121 | 5.23e-66 | 196 |
| MS.gene55884.t1 | AT4G29480 | 73.950 | 119 | 31 | 0 | 2 | 120 | 3 | 121 | 9.15e-66 | 195 |
| MS.gene55884.t1 | AT2G19680 | 73.950 | 119 | 31 | 0 | 2 | 120 | 3 | 121 | 2.46e-65 | 194 |
| MS.gene55884.t1 | AT2G19680 | 73.950 | 119 | 31 | 0 | 2 | 120 | 3 | 121 | 2.46e-65 | 194 |
Find 23 sgRNAs with CRISPR-Local
Find 116 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCATGCTTAGTTATAAAT+TGG | 0.149886 | 4.3:+3501183 | None:intergenic |
| TTGATTATGTCAAGCAAATA+TGG | 0.268577 | 4.3:-3499449 | MS.gene55884:CDS |
| TGAAAGGACTCACAACGATT+TGG | 0.345795 | 4.3:+3499480 | None:intergenic |
| AAAACCATGCATAGCATTCC+AGG | 0.362552 | 4.3:+3499373 | None:intergenic |
| TCCATGCTTAGTTATAAATT+GGG | 0.411328 | 4.3:+3501184 | None:intergenic |
| GAAGAACAAGGAATACATTC+AGG | 0.438061 | 4.3:-3501135 | MS.gene55884:CDS |
| GGAAGGGGATTTACTTTCAC+AGG | 0.448226 | 4.3:-3499333 | MS.gene55884:CDS |
| TGGCATTTCTCTACTGTAGC+AGG | 0.457610 | 4.3:+3501110 | None:intergenic |
| TTCTTACCACAAGCAGCTGT+TGG | 0.459464 | 4.3:-3501159 | MS.gene55884:CDS |
| TCGTTGTGAGTCCTTTCATA+AGG | 0.471099 | 4.3:-3499475 | MS.gene55884:CDS |
| TGCTGGTCAGATTGTTGGAA+GGG | 0.499361 | 4.3:-3499349 | MS.gene55884:CDS |
| AGTAGAGAAATGCCAACTTC+TGG | 0.523939 | 4.3:-3501102 | MS.gene55884:CDS |
| ATAGTTGCTTGGCCAGAAGT+TGG | 0.524773 | 4.3:+3501090 | None:intergenic |
| TCCCAATTTATAACTAAGCA+TGG | 0.525968 | 4.3:-3501185 | MS.gene55884:CDS |
| ATAATCAAGTTCCTTATGAA+AGG | 0.546893 | 4.3:+3499464 | None:intergenic |
| GCTGGTCAGATTGTTGGAAG+GGG | 0.563270 | 4.3:-3499348 | MS.gene55884:CDS |
| GGTTAAAATAAAACCTAGCA+AGG | 0.573767 | 4.3:+3501057 | None:intergenic |
| GTGCTGGTCAGATTGTTGGA+AGG | 0.577540 | 4.3:-3499350 | MS.gene55884:CDS |
| GCGAGTGTAAAATAGTTGCT+TGG | 0.582756 | 4.3:+3501079 | None:intergenic |
| TCTTCTCCAACAGCTGCTTG+TGG | 0.584533 | 4.3:+3501153 | None:intergenic |
| TTGGCCTGGAATGCTATGCA+TGG | 0.593962 | 4.3:-3499377 | MS.gene55884:CDS |
| GATTTGAAGATTGAAGATGC+AGG | 0.608817 | 4.3:-3499414 | MS.gene55884:CDS |
| GCAGCTGTTGGAGAAGAACA+AGG | 0.648148 | 4.3:-3501147 | MS.gene55884:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTTAAAAAAAAAAAAATTG+AGG | + | chr4.3:3500025-3500044 | None:intergenic | 10.0% |
| !! | TAGATTTATTAATAAGGAAA+GGG | + | chr4.3:3499514-3499533 | None:intergenic | 15.0% |
| !! | TCTTTAATTATTAAGTATGA+TGG | - | chr4.3:3500196-3500215 | MS.gene55884:intron | 15.0% |
| !!! | GTTTTATTAAAGAGTTAAAA+GGG | - | chr4.3:3500046-3500065 | MS.gene55884:intron | 15.0% |
| !!! | TGTTTTATTAAAGAGTTAAA+AGG | - | chr4.3:3500045-3500064 | MS.gene55884:intron | 15.0% |
| !! | AAAAATCACTTACAATCAAT+AGG | + | chr4.3:3499581-3499600 | None:intergenic | 20.0% |
| !! | AATAGAAACAAACTAAAAGT+AGG | + | chr4.3:3500131-3500150 | None:intergenic | 20.0% |
| !! | CTAGATTTATTAATAAGGAA+AGG | + | chr4.3:3499515-3499534 | None:intergenic | 20.0% |
| !! | TAGAAAAATACACGAAATTA+TGG | + | chr4.3:3500225-3500244 | None:intergenic | 20.0% |
| !! | TATACAGTATAAAAGATGAT+CGG | - | chr4.3:3500383-3500402 | MS.gene55884:intron | 20.0% |
| !!! | TTTTTTTTGCATTTTGATGT+CGG | - | chr4.3:3499601-3499620 | MS.gene55884:intron | 20.0% |
| ! | ATAATCAAGTTCCTTATGAA+AGG | + | chr4.3:3501086-3501105 | None:intergenic | 25.0% |
| ! | ATACAGTATAAAAGATGATC+GGG | - | chr4.3:3500384-3500403 | MS.gene55884:intron | 25.0% |
| ! | ATAGCTAAAAGAAGAAACTA+CGG | + | chr4.3:3500962-3500981 | None:intergenic | 25.0% |
| ! | GATTGAACAATCAACATATT+AGG | + | chr4.3:3499706-3499725 | None:intergenic | 25.0% |
| ! | TCCAGCTAGATTTATTAATA+AGG | + | chr4.3:3499520-3499539 | None:intergenic | 25.0% |
| ! | TCCATGCTTAGTTATAAATT+GGG | + | chr4.3:3499366-3499385 | None:intergenic | 25.0% |
| ! | TCCTTATTAATAAATCTAGC+TGG | - | chr4.3:3499516-3499535 | MS.gene55884:intron | 25.0% |
| ! | TTCCATGCTTAGTTATAAAT+TGG | + | chr4.3:3499367-3499386 | None:intergenic | 25.0% |
| ! | TTGATTATGTCAAGCAAATA+TGG | - | chr4.3:3501098-3501117 | MS.gene55884:CDS | 25.0% |
| ! | TTGTACTAAATAGCTTATTG+TGG | + | chr4.3:3500541-3500560 | None:intergenic | 25.0% |
| !! | ATATACCACTATAGCATTTT+AGG | - | chr4.3:3500310-3500329 | MS.gene55884:intron | 25.0% |
| !! | TATACCACTATAGCATTTTA+GGG | - | chr4.3:3500311-3500330 | MS.gene55884:intron | 25.0% |
| !!! | GTTTTGTTGTTTTGTACTTT+AGG | - | chr4.3:3500172-3500191 | MS.gene55884:intron | 25.0% |
| ACCATTAACTTTGTAGTTCA+GGG | + | chr4.3:3499747-3499766 | None:intergenic | 30.0% | |
| GGTTAAAATAAAACCTAGCA+AGG | + | chr4.3:3499493-3499512 | None:intergenic | 30.0% | |
| GTGAACTCTAAACTAACTAA+GGG | + | chr4.3:3500474-3500493 | None:intergenic | 30.0% | |
| TAAGACTTCATTGCTAAGAA+AGG | + | chr4.3:3500432-3500451 | None:intergenic | 30.0% | |
| TACAAAATACTGTGCAATAG+TGG | - | chr4.3:3500672-3500691 | MS.gene55884:intron | 30.0% | |
| TATAGTGGTATATCGAATAG+CGG | + | chr4.3:3500303-3500322 | None:intergenic | 30.0% | |
| TCCCAATTTATAACTAAGCA+TGG | - | chr4.3:3499362-3499381 | MS.gene55884:CDS | 30.0% | |
| TCGCTAATATATGTACTTGT+TGG | - | chr4.3:3499555-3499574 | MS.gene55884:intron | 30.0% | |
| TGTGAACTCTAAACTAACTA+AGG | + | chr4.3:3500475-3500494 | None:intergenic | 30.0% | |
| TGTTGAATTGAAACATCAGA+AGG | - | chr4.3:3500857-3500876 | MS.gene55884:intron | 30.0% | |
| TGTTGAATTGAAACATCAGA+TGG | - | chr4.3:3500857-3500876 | MS.gene55884:intron | 30.0% | |
| TTGTACTAAATAGCGTATTG+CGG | + | chr4.3:3500657-3500676 | None:intergenic | 30.0% | |
| ! | ATACCACTATAGCATTTTAG+GGG | - | chr4.3:3500312-3500331 | MS.gene55884:intron | 30.0% |
| ! | ATTTTCTCCCTTGTCAATAA+AGG | + | chr4.3:3499655-3499674 | None:intergenic | 30.0% |
| ! | TTTTCTCCCTTGTCAATAAA+GGG | + | chr4.3:3499654-3499673 | None:intergenic | 30.0% |
| AATATGACACTGTTGCATAG+TGG | - | chr4.3:3500705-3500724 | MS.gene55884:intron | 35.0% | |
| AATCCCCTAAAATGCTATAG+TGG | + | chr4.3:3500318-3500337 | None:intergenic | 35.0% | |
| ACACTGTGAACTCTAAACTA+AGG | + | chr4.3:3500479-3500498 | None:intergenic | 35.0% | |
| AGCCAATTACAGTGAATTTG+CGG | - | chr4.3:3499953-3499972 | MS.gene55884:intron | 35.0% | |
| ATAGTGGTATATCGAATAGC+GGG | + | chr4.3:3500302-3500321 | None:intergenic | 35.0% | |
| ATCACATGAACCCTAATTCA+TGG | + | chr4.3:3500809-3500828 | None:intergenic | 35.0% | |
| CACTGTGAACTCTAAACTAA+GGG | + | chr4.3:3500478-3500497 | None:intergenic | 35.0% | |
| CCATTAACTTTGTAGTTCAG+GGG | + | chr4.3:3499746-3499765 | None:intergenic | 35.0% | |
| GAAGAACAAGGAATACATTC+AGG | - | chr4.3:3499412-3499431 | MS.gene55884:CDS | 35.0% | |
| GACCATTAACTTTGTAGTTC+AGG | + | chr4.3:3499748-3499767 | None:intergenic | 35.0% | |
| GATTTGAAGATTGAAGATGC+AGG | - | chr4.3:3501133-3501152 | MS.gene55884:CDS | 35.0% | |
| TATTGATCAGAATTCCCATG+TGG | - | chr4.3:3500918-3500937 | MS.gene55884:intron | 35.0% | |
| TGTTGGATATCATAGAGTTC+AGG | - | chr4.3:3500783-3500802 | MS.gene55884:intron | 35.0% | |
| !! | ACTATAGCATTTTAGGGGAT+TGG | - | chr4.3:3500317-3500336 | MS.gene55884:intron | 35.0% |
| AAAACCATGCATAGCATTCC+AGG | + | chr4.3:3501177-3501196 | None:intergenic | 40.0% | |
| AGAGTTCAGGCCATGAATTA+GGG | - | chr4.3:3500796-3500815 | MS.gene55884:intron | 40.0% | |
| AGTAGAGAAATGCCAACTTC+TGG | - | chr4.3:3499445-3499464 | MS.gene55884:CDS | 40.0% | |
| ATTGACAAGGGAGAAAATCC+TGG | - | chr4.3:3499657-3499676 | MS.gene55884:intron | 40.0% | |
| CCCCTGAACTACAAAGTTAA+TGG | - | chr4.3:3499743-3499762 | MS.gene55884:intron | 40.0% | |
| GCGAGTGTAAAATAGTTGCT+TGG | + | chr4.3:3499471-3499490 | None:intergenic | 40.0% | |
| TAGAGTTCAGGCCATGAATT+AGG | - | chr4.3:3500795-3500814 | MS.gene55884:intron | 40.0% | |
| TCGTTGTGAGTCCTTTCATA+AGG | - | chr4.3:3501072-3501091 | MS.gene55884:CDS | 40.0% | |
| TGAAAGGACTCACAACGATT+TGG | + | chr4.3:3501070-3501089 | None:intergenic | 40.0% | |
| TGATATCCAACACTGAACAC+AGG | + | chr4.3:3500775-3500794 | None:intergenic | 40.0% | |
| TTCCCTCCCTTTATTGACAA+GGG | - | chr4.3:3499645-3499664 | MS.gene55884:intron | 40.0% | |
| TTGTCAATAAAGGGAGGGAA+GGG | + | chr4.3:3499645-3499664 | None:intergenic | 40.0% | |
| TTGTCAATAGCGTGCTATAG+CGG | - | chr4.3:3499844-3499863 | MS.gene55884:intron | 40.0% | |
| ! | TATTGCTGACTGTGGTTTGT+GGG | + | chr4.3:3499919-3499938 | None:intergenic | 40.0% |
| ! | TATTTTACACTCGCCTTGCT+AGG | - | chr4.3:3499477-3499496 | MS.gene55884:CDS | 40.0% |
| !! | TTTTGTGCTGGTCAGATTGT+TGG | - | chr4.3:3501193-3501212 | MS.gene55884:CDS | 40.0% |
| !!! | GCAGGTATTGCTGTTTTGTT+TGG | - | chr4.3:3501151-3501170 | MS.gene55884:CDS | 40.0% |
| !!! | TATTGCTGTTTTGTTTGGCC+TGG | - | chr4.3:3501156-3501175 | MS.gene55884:CDS | 40.0% |
| AACACAGGTGTAGTCAACTG+TGG | + | chr4.3:3500760-3500779 | None:intergenic | 45.0% | |
| AACGTATCCGTGGTAGGATT+TGG | - | chr4.3:3499870-3499889 | MS.gene55884:intron | 45.0% | |
| ACAAGGGAGAAAATCCTGGA+GGG | - | chr4.3:3499661-3499680 | MS.gene55884:intron | 45.0% | |
| ACTACACCTGTGTTCAGTGT+TGG | - | chr4.3:3500766-3500785 | MS.gene55884:intron | 45.0% | |
| AGCAGACACGATAGCAGTTT+GGG | + | chr4.3:3499980-3499999 | None:intergenic | 45.0% | |
| ATAGTTGCTTGGCCAGAAGT+TGG | + | chr4.3:3499460-3499479 | None:intergenic | 45.0% | |
| ATCCGTGGTAGGATTTGGAA+CGG | - | chr4.3:3499875-3499894 | MS.gene55884:intron | 45.0% | |
| CAAACCACAGTCAGCAATAG+TGG | - | chr4.3:3499920-3499939 | MS.gene55884:intron | 45.0% | |
| CTCCCTTGTCAATAAAGGGA+GGG | + | chr4.3:3499650-3499669 | None:intergenic | 45.0% | |
| CTGTGCAATAGTGGCTATAG+CGG | - | chr4.3:3500681-3500700 | MS.gene55884:intron | 45.0% | |
| CTTCCCTCCCTTTATTGACA+AGG | - | chr4.3:3499644-3499663 | MS.gene55884:intron | 45.0% | |
| CTTGTCAATAAAGGGAGGGA+AGG | + | chr4.3:3499646-3499665 | None:intergenic | 45.0% | |
| GGAAGGGGATTTACTTTCAC+AGG | - | chr4.3:3501214-3501233 | MS.gene55884:CDS | 45.0% | |
| GGCCGCAAATTCACTGTAAT+TGG | + | chr4.3:3499958-3499977 | None:intergenic | 45.0% | |
| TAGCAGACACGATAGCAGTT+TGG | + | chr4.3:3499981-3500000 | None:intergenic | 45.0% | |
| TCTCCCTTGTCAATAAAGGG+AGG | + | chr4.3:3499651-3499670 | None:intergenic | 45.0% | |
| TGCTGGTCAGATTGTTGGAA+GGG | - | chr4.3:3501198-3501217 | MS.gene55884:CDS | 45.0% | |
| TGGCATTTCTCTACTGTAGC+AGG | + | chr4.3:3499440-3499459 | None:intergenic | 45.0% | |
| TTCTTACCACAAGCAGCTGT+TGG | - | chr4.3:3499388-3499407 | MS.gene55884:CDS | 45.0% | |
| ! | ATAGCGGAATAACGTATCCG+TGG | - | chr4.3:3499860-3499879 | MS.gene55884:intron | 45.0% |
| ! | CTATTGCTGACTGTGGTTTG+TGG | + | chr4.3:3499920-3499939 | None:intergenic | 45.0% |
| !! | GCCTCTAAAAAGCACCACAT+GGG | + | chr4.3:3500935-3500954 | None:intergenic | 45.0% |
| !! | TCCCATGTGGTGCTTTTTAG+AGG | - | chr4.3:3500931-3500950 | MS.gene55884:intron | 45.0% |
| !! | TGCCTCTAAAAAGCACCACA+TGG | + | chr4.3:3500936-3500955 | None:intergenic | 45.0% |
| !!! | TGCTATGCATGGTTTTGTGC+TGG | - | chr4.3:3501181-3501200 | MS.gene55884:CDS | 45.0% |
| AAGATGATCGGGTGATCGTG+TGG | - | chr4.3:3500395-3500414 | MS.gene55884:intron | 50.0% | |
| ACCACAGTCAGCAATAGTGG+TGG | - | chr4.3:3499923-3499942 | MS.gene55884:intron | 50.0% | |
| CGGAATAACGTATCCGTGGT+AGG | - | chr4.3:3499864-3499883 | MS.gene55884:intron | 50.0% | |
| CTGCTATCGTGTCTGCTAGT+AGG | - | chr4.3:3499983-3500002 | MS.gene55884:intron | 50.0% | |
| GACAAGGGAGAAAATCCTGG+AGG | - | chr4.3:3499660-3499679 | MS.gene55884:intron | 50.0% | |
| GCAGACACGATAGCAGTTTG+GGG | + | chr4.3:3499979-3499998 | None:intergenic | 50.0% | |
| GCAGCTGTTGGAGAAGAACA+AGG | - | chr4.3:3499400-3499419 | MS.gene55884:CDS | 50.0% | |
| GCTGGTCAGATTGTTGGAAG+GGG | - | chr4.3:3501199-3501218 | MS.gene55884:CDS | 50.0% | |
| GTCCGTTCCAAATCCTACCA+CGG | + | chr4.3:3499880-3499899 | None:intergenic | 50.0% | |
| TCTTCTCCAACAGCTGCTTG+TGG | + | chr4.3:3499397-3499416 | None:intergenic | 50.0% | |
| TTGGCCTGGAATGCTATGCA+TGG | - | chr4.3:3501170-3501189 | MS.gene55884:CDS | 50.0% | |
| ! | ACCACCACTATTGCTGACTG+TGG | + | chr4.3:3499927-3499946 | None:intergenic | 50.0% |
| ! | GGGATTGGTGCATGCTATCT+GGG | - | chr4.3:3500332-3500351 | MS.gene55884:intron | 50.0% |
| ! | GTGCTGGTCAGATTGTTGGA+AGG | - | chr4.3:3501197-3501216 | MS.gene55884:CDS | 50.0% |
| AACTTGAAGCCTCACCCTCC+AGG | + | chr4.3:3499678-3499697 | None:intergenic | 55.0% | |
| GGAGAAAATCCTGGAGGGTG+AGG | - | chr4.3:3499666-3499685 | MS.gene55884:intron | 55.0% | |
| ! | AGGATTTGGAACGGACCGCA+CGG | - | chr4.3:3499884-3499903 | MS.gene55884:intron | 55.0% |
| ! | GGGGATTGGTGCATGCTATC+TGG | - | chr4.3:3500331-3500350 | MS.gene55884:intron | 55.0% |
| ! | AACGGACCGCACGGTGCTTT+TGG | - | chr4.3:3499893-3499912 | MS.gene55884:intron | 60.0% |
| !! | TGTGGGCCAAAAGCACCGTG+CGG | + | chr4.3:3499902-3499921 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 3499320 | 3501249 | 3499320 | ID=MS.gene55884 |
| chr4.3 | mRNA | 3499320 | 3501249 | 3499320 | ID=MS.gene55884.t1;Parent=MS.gene55884 |
| chr4.3 | exon | 3501071 | 3501249 | 3501071 | ID=MS.gene55884.t1.exon1;Parent=MS.gene55884.t1 |
| chr4.3 | CDS | 3501071 | 3501249 | 3501071 | ID=cds.MS.gene55884.t1;Parent=MS.gene55884.t1 |
| chr4.3 | exon | 3499320 | 3499506 | 3499320 | ID=MS.gene55884.t1.exon2;Parent=MS.gene55884.t1 |
| chr4.3 | CDS | 3499320 | 3499506 | 3499320 | ID=cds.MS.gene55884.t1;Parent=MS.gene55884.t1 |
| Gene Sequence |
| Protein sequence |
