Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56004.t1 | RHN73070.1 | 96.2 | 52 | 0 | 1 | 1 | 52 | 1 | 50 | 5.00E-16 | 92.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56004.t1 | A0A396J551 | 96.2 | 52 | 0 | 1 | 1 | 52 | 1 | 50 | 3.6e-16 | 92.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049669 | MS.gene56004 | 0.801161 | 1.03E-48 | -1.69E-46 |
| MS.gene050680 | MS.gene56004 | 0.805611 | 1.23E-49 | -1.69E-46 |
| MS.gene050953 | MS.gene56004 | 0.8087 | 2.72E-50 | -1.69E-46 |
| MS.gene052586 | MS.gene56004 | 0.803005 | 4.28E-49 | -1.69E-46 |
| MS.gene054826 | MS.gene56004 | 0.814156 | 1.78E-51 | -1.69E-46 |
| MS.gene055701 | MS.gene56004 | 0.826121 | 3.23E-54 | -1.69E-46 |
| MS.gene059128 | MS.gene56004 | 0.822163 | 2.74E-53 | -1.69E-46 |
| MS.gene059382 | MS.gene56004 | 0.802749 | 4.84E-49 | -1.69E-46 |
| MS.gene060322 | MS.gene56004 | 0.800195 | 1.62E-48 | -1.69E-46 |
| MS.gene060879 | MS.gene56004 | 0.815955 | 7.09E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 10 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACTTTGAATTTGAAGCAAT+CGG | 0.406582 | 2.3:+56396646 | None:intergenic |
| CTCAACTGTTGATCTTTGCA+CGG | 0.434979 | 2.3:+56396517 | None:intergenic |
| CATTCAACAACCATGGCAAC+GGG | 0.455582 | 2.3:-56396554 | MS.gene56004:CDS |
| TGAGTTTGCAGTGCCATGAT+TGG | 0.461841 | 2.3:+56396575 | None:intergenic |
| AGTCTTGCTTCCCGTTGCCA+TGG | 0.528592 | 2.3:+56396544 | None:intergenic |
| TCATTCAACAACCATGGCAA+CGG | 0.561765 | 2.3:-56396555 | MS.gene56004:CDS |
| TCAACTGTTGATCTTTGCAC+GGG | 0.562450 | 2.3:+56396518 | None:intergenic |
| AAACTTCTTGAGACTGAGGA+AGG | 0.625104 | 2.3:-56396618 | MS.gene56004:CDS |
| GCAAACTCATTCAACAACCA+TGG | 0.640162 | 2.3:-56396561 | MS.gene56004:CDS |
| ACGCAAACTTCTTGAGACTG+AGG | 0.679118 | 2.3:-56396622 | MS.gene56004:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GACTTTGAATTTGAAGCAAT+CGG | + | chr2.3:56396529-56396548 | None:intergenic | 30.0% |
| ! | GAACTTTTCAACTCCAATCA+TGG | - | chr2.3:56396584-56396603 | MS.gene56004:CDS | 35.0% |
| AAACTTCTTGAGACTGAGGA+AGG | - | chr2.3:56396554-56396573 | MS.gene56004:CDS | 40.0% | |
| GCAAACTCATTCAACAACCA+TGG | - | chr2.3:56396611-56396630 | MS.gene56004:CDS | 40.0% | |
| TCAACTGTTGATCTTTGCAC+GGG | + | chr2.3:56396657-56396676 | None:intergenic | 40.0% | |
| TCATTCAACAACCATGGCAA+CGG | - | chr2.3:56396617-56396636 | MS.gene56004:CDS | 40.0% | |
| ACGCAAACTTCTTGAGACTG+AGG | - | chr2.3:56396550-56396569 | MS.gene56004:CDS | 45.0% | |
| CATTCAACAACCATGGCAAC+GGG | - | chr2.3:56396618-56396637 | MS.gene56004:CDS | 45.0% | |
| TGAGTTTGCAGTGCCATGAT+TGG | + | chr2.3:56396600-56396619 | None:intergenic | 45.0% | |
| AGTCTTGCTTCCCGTTGCCA+TGG | + | chr2.3:56396631-56396650 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 56396518 | 56396676 | 56396518 | ID=MS.gene56004 |
| chr2.3 | mRNA | 56396518 | 56396676 | 56396518 | ID=MS.gene56004.t1;Parent=MS.gene56004 |
| chr2.3 | exon | 56396518 | 56396676 | 56396518 | ID=MS.gene56004.t1.exon1;Parent=MS.gene56004.t1 |
| chr2.3 | CDS | 56396518 | 56396676 | 56396518 | ID=cds.MS.gene56004.t1;Parent=MS.gene56004.t1 |
| Gene Sequence |
| Protein sequence |
