AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059382


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059382.t1 MTR_6g048070 86.000 100 12 1 1 100 1 98 1.01e-53 168
MS.gene059382.t1 MTR_6g013220 39.610 154 65 2 93 218 55 208 1.54e-25 99.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 53 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAAATGCTTTGAAAATTTA+TGG 0.103412 6.3:-42890715 None:intergenic
AGGAATTATTATGAATATTT+TGG 0.109615 6.3:+42890157 None:intergenic
ACCAAGAGCCATCTCTTATT+TGG 0.186353 6.3:-42890491 None:intergenic
CCATACGCCCGTTTGTTCTT+AGG 0.263802 6.3:+42890276 MS.gene059382:CDS
CTCACTGAGGAAACGGTTAA+AGG 0.312213 6.3:+42890344 MS.gene059382:CDS
GGATTACTTGGTTTGGAAAC+AGG 0.343584 6.3:+42890195 MS.gene059382:CDS
GAAGACAATTTCAAATCCTT+TGG 0.353154 6.3:+42890622 MS.gene059382:CDS
GTCACACGGATTACTTGGTT+TGG 0.363011 6.3:+42890188 MS.gene059382:CDS
GTTCGGAAATGGTTGGGATC+AGG 0.375886 6.3:+42890740 MS.gene059382:CDS
TTCAAATCCTTTGGTGGTGA+TGG 0.384513 6.3:+42890631 MS.gene059382:CDS
GTAAAGAGAGAGATAATTTA+CGG 0.394352 6.3:+42890579 MS.gene059382:CDS
ACTTGGTTTGGAAACAGGTT+GGG 0.398496 6.3:+42890200 MS.gene059382:CDS
GTCTACAAAATACTCAAAAT+CGG 0.406722 6.3:-42890693 None:intergenic
TGAATGTCACACGGATTACT+TGG 0.411945 6.3:+42890183 MS.gene059382:CDS
GGGTTGATTCGGTTAGGACC+AGG 0.413078 6.3:+42890221 MS.gene059382:CDS
ACCAAATAAGAGATGGCTCT+TGG 0.414703 6.3:+42890490 MS.gene059382:CDS
TCCACCAATCCCATCACAAC+TGG 0.419830 6.3:-42890305 None:intergenic
GAAACAGGTTGGGGTTGATT+CGG 0.433871 6.3:+42890210 MS.gene059382:CDS
TTTAAGGTTCGGAAATGGTT+GGG 0.445226 6.3:+42890734 MS.gene059382:CDS
GGAGTGATTTGCTGAACTTA+AGG 0.461995 6.3:+42890600 MS.gene059382:CDS
GACAATTTCAAATCCTTTGG+TGG 0.464703 6.3:+42890625 MS.gene059382:CDS
GGATTATACCTAAGAACAAA+CGG 0.466483 6.3:-42890284 None:intergenic
AAGAGATGGCTCTTGGTTGA+AGG 0.467671 6.3:+42890497 MS.gene059382:CDS
TCATAACAATGAAGCACTAA+AGG 0.476464 6.3:-42890785 None:intergenic
CATAACAATGAAGCACTAAA+GGG 0.476728 6.3:-42890784 None:intergenic
AGGTTGGGGTTGATTCGGTT+AGG 0.490838 6.3:+42890215 MS.gene059382:CDS
TCAAATCCTTTGGTGGTGAT+GGG 0.493046 6.3:+42890632 MS.gene059382:CDS
AGAAGGAAGACAATTTAAGC+TGG 0.499740 6.3:+42890375 MS.gene059382:CDS
TAGGTATAATCCAGTTGTGA+TGG 0.501054 6.3:+42890295 MS.gene059382:CDS
CTTGGTTTGGAAACAGGTTG+GGG 0.501383 6.3:+42890201 MS.gene059382:CDS
TACTTGGTTTGGAAACAGGT+TGG 0.506523 6.3:+42890199 MS.gene059382:CDS
TTCGGAAATGGTTGGGATCA+GGG 0.508284 6.3:+42890741 MS.gene059382:CDS
TCTCTCTCTTTACGACAACT+TGG 0.508403 6.3:-42890569 None:intergenic
GGGGAAAGTATTACTCAGTC+AGG 0.520760 6.3:+42890242 MS.gene059382:CDS
TTCGAAGCTCACTGAGGAAA+CGG 0.521223 6.3:+42890337 MS.gene059382:CDS
CCTAAGAACAAACGGGCGTA+TGG 0.530528 6.3:-42890276 None:intergenic
GGTTAAAGGATGTTTGAAGA+AGG 0.530922 6.3:+42890358 MS.gene059382:CDS
TCCAGTTGTGATGGGATTGG+TGG 0.539138 6.3:+42890304 MS.gene059382:CDS
AGGTATAATCCAGTTGTGAT+GGG 0.541100 6.3:+42890296 MS.gene059382:CDS
GATTATACCTAAGAACAAAC+GGG 0.541259 6.3:-42890283 None:intergenic
TTCAAAGACAAGAAAAGTGG+GGG 0.560074 6.3:+42890419 MS.gene059382:CDS
GGATCAGGGAGAGTCCGCTC+AGG 0.570190 6.3:+42890755 MS.gene059382:CDS
AACTTACCCATCACCACCAA+AGG 0.574656 6.3:-42890638 None:intergenic
GTTGATTCGGTTAGGACCAG+GGG 0.579094 6.3:+42890223 MS.gene059382:CDS
GGTTGATTCGGTTAGGACCA+GGG 0.584355 6.3:+42890222 MS.gene059382:CDS
GAAGGAAGACAATTTAAGCT+GGG 0.584879 6.3:+42890376 MS.gene059382:CDS
GACTGAGTAATACTTTCCCC+TGG 0.585428 6.3:-42890239 None:intergenic
AAACTTTACCAAATAAGAGA+TGG 0.585590 6.3:+42890483 MS.gene059382:CDS
TAATCCAGTTGTGATGGGAT+TGG 0.599371 6.3:+42890301 MS.gene059382:CDS
TTTCAAAGACAAGAAAAGTG+GGG 0.614665 6.3:+42890418 MS.gene059382:CDS
AACGGGCGTATGGACTGTGA+TGG 0.658353 6.3:-42890266 None:intergenic
CTAAAGGGCGCTGACCTGAG+CGG 0.669360 6.3:-42890769 None:intergenic
AAGACGTTCGAAGCTCACTG+AGG 0.781877 6.3:+42890331 MS.gene059382:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAATGCTTTGAAAATTTA+TGG - chr6.3:42890718-42890737 None:intergenic 15.0%
!!! TAAATTTTCAAAGCATTTTA+AGG + chr6.3:42890718-42890737 MS.gene059382:CDS 15.0%
!!! TTGTCTTTGAAAAATGATTT+TGG - chr6.3:42890410-42890429 None:intergenic 20.0%
! AAACTTTACCAAATAAGAGA+TGG + chr6.3:42890483-42890502 MS.gene059382:CDS 25.0%
! GTAAAGAGAGAGATAATTTA+CGG + chr6.3:42890579-42890598 MS.gene059382:CDS 25.0%
! GTCTACAAAATACTCAAAAT+CGG - chr6.3:42890696-42890715 None:intergenic 25.0%
!! TATTGATGTGTCTTTTCTTT+TGG + chr6.3:42890538-42890557 MS.gene059382:CDS 25.0%
!! TTTTCAAAGACAAGAAAAGT+GGG + chr6.3:42890417-42890436 MS.gene059382:CDS 25.0%
!!! TTTCAAAGCATTTTAAGGTT+CGG + chr6.3:42890723-42890742 MS.gene059382:CDS 25.0%
!!! TTTTTCAAAGACAAGAAAAG+TGG + chr6.3:42890416-42890435 MS.gene059382:CDS 25.0%
GAAGACAATTTCAAATCCTT+TGG + chr6.3:42890622-42890641 MS.gene059382:CDS 30.0%
GATTATACCTAAGAACAAAC+GGG - chr6.3:42890286-42890305 None:intergenic 30.0%
GGATTATACCTAAGAACAAA+CGG - chr6.3:42890287-42890306 None:intergenic 30.0%
TTTCAAAGACAAGAAAAGTG+GGG + chr6.3:42890418-42890437 MS.gene059382:CDS 30.0%
! CATAACAATGAAGCACTAAA+GGG - chr6.3:42890787-42890806 None:intergenic 30.0%
! TCATAACAATGAAGCACTAA+AGG - chr6.3:42890788-42890807 None:intergenic 30.0%
!! AAAATCGGCTTCTTTTTCTT+CGG - chr6.3:42890681-42890700 None:intergenic 30.0%
AGAAGGAAGACAATTTAAGC+TGG + chr6.3:42890375-42890394 MS.gene059382:CDS 35.0%
AGGTATAATCCAGTTGTGAT+GGG + chr6.3:42890296-42890315 MS.gene059382:CDS 35.0%
GAAGGAAGACAATTTAAGCT+GGG + chr6.3:42890376-42890395 MS.gene059382:CDS 35.0%
GACAATTTCAAATCCTTTGG+TGG + chr6.3:42890625-42890644 MS.gene059382:CDS 35.0%
GGTTAAAGGATGTTTGAAGA+AGG + chr6.3:42890358-42890377 MS.gene059382:CDS 35.0%
TAGGTATAATCCAGTTGTGA+TGG + chr6.3:42890295-42890314 MS.gene059382:CDS 35.0%
TTCAAAGACAAGAAAAGTGG+GGG + chr6.3:42890419-42890438 MS.gene059382:CDS 35.0%
! TTTAAGGTTCGGAAATGGTT+GGG + chr6.3:42890734-42890753 MS.gene059382:CDS 35.0%
!!! AGCATTTTAAGGTTCGGAAA+TGG + chr6.3:42890729-42890748 MS.gene059382:CDS 35.0%
!!! GTGATGGGTAAGTTTTATGA+TGG + chr6.3:42890647-42890666 MS.gene059382:CDS 35.0%
!!! TTTTAAGGTTCGGAAATGGT+TGG + chr6.3:42890733-42890752 MS.gene059382:CDS 35.0%
!!! TTTTGGGTTTGAATGTCACA+CGG + chr6.3:42890174-42890193 MS.gene059382:CDS 35.0%
ACCAAATAAGAGATGGCTCT+TGG + chr6.3:42890490-42890509 MS.gene059382:CDS 40.0%
ACCAAGAGCCATCTCTTATT+TGG - chr6.3:42890494-42890513 None:intergenic 40.0%
TCTCTCTCTTTACGACAACT+TGG - chr6.3:42890572-42890591 None:intergenic 40.0%
! TAATCCAGTTGTGATGGGAT+TGG + chr6.3:42890301-42890320 MS.gene059382:CDS 40.0%
! TGAATGTCACACGGATTACT+TGG + chr6.3:42890183-42890202 MS.gene059382:CDS 40.0%
!! ACTTGGTTTGGAAACAGGTT+GGG + chr6.3:42890200-42890219 MS.gene059382:CDS 40.0%
!! GGAGTGATTTGCTGAACTTA+AGG + chr6.3:42890600-42890619 MS.gene059382:CDS 40.0%
!! GGATTACTTGGTTTGGAAAC+AGG + chr6.3:42890195-42890214 MS.gene059382:CDS 40.0%
!! TACTTGGTTTGGAAACAGGT+TGG + chr6.3:42890199-42890218 MS.gene059382:CDS 40.0%
!! TCAAATCCTTTGGTGGTGAT+GGG + chr6.3:42890632-42890651 MS.gene059382:CDS 40.0%
!! TTCAAATCCTTTGGTGGTGA+TGG + chr6.3:42890631-42890650 MS.gene059382:CDS 40.0%
AACTTACCCATCACCACCAA+AGG - chr6.3:42890641-42890660 None:intergenic 45.0%
AAGAGATGGCTCTTGGTTGA+AGG + chr6.3:42890497-42890516 MS.gene059382:CDS 45.0%
CTCACTGAGGAAACGGTTAA+AGG + chr6.3:42890344-42890363 MS.gene059382:CDS 45.0%
GACTGAGTAATACTTTCCCC+TGG - chr6.3:42890242-42890261 None:intergenic 45.0%
TTCGAAGCTCACTGAGGAAA+CGG + chr6.3:42890337-42890356 MS.gene059382:CDS 45.0%
TTCGGAAATGGTTGGGATCA+GGG + chr6.3:42890741-42890760 MS.gene059382:CDS 45.0%
! GAAACAGGTTGGGGTTGATT+CGG + chr6.3:42890210-42890229 MS.gene059382:CDS 45.0%
! GTCACACGGATTACTTGGTT+TGG + chr6.3:42890188-42890207 MS.gene059382:CDS 45.0%
! TCGGCTTCTTTTTCTTCGGA+TGG - chr6.3:42890677-42890696 None:intergenic 45.0%
!! CTTGGTTTGGAAACAGGTTG+GGG + chr6.3:42890201-42890220 MS.gene059382:CDS 45.0%
!! GGGGAAAGTATTACTCAGTC+AGG + chr6.3:42890242-42890261 MS.gene059382:CDS 45.0%
AAGACGTTCGAAGCTCACTG+AGG + chr6.3:42890331-42890350 MS.gene059382:CDS 50.0%
CCATACGCCCGTTTGTTCTT+AGG + chr6.3:42890276-42890295 MS.gene059382:CDS 50.0%
CCTAAGAACAAACGGGCGTA+TGG - chr6.3:42890279-42890298 None:intergenic 50.0%
GTTCGGAAATGGTTGGGATC+AGG + chr6.3:42890740-42890759 MS.gene059382:CDS 50.0%
TCCACCAATCCCATCACAAC+TGG - chr6.3:42890308-42890327 None:intergenic 50.0%
! TCCAGTTGTGATGGGATTGG+TGG + chr6.3:42890304-42890323 MS.gene059382:CDS 50.0%
!! AGGTTGGGGTTGATTCGGTT+AGG + chr6.3:42890215-42890234 MS.gene059382:CDS 50.0%
!! GGTTGATTCGGTTAGGACCA+GGG + chr6.3:42890222-42890241 MS.gene059382:CDS 50.0%
!! GTTGATTCGGTTAGGACCAG+GGG + chr6.3:42890223-42890242 MS.gene059382:CDS 50.0%
! AACGGGCGTATGGACTGTGA+TGG - chr6.3:42890269-42890288 None:intergenic 55.0%
!! GGGTTGATTCGGTTAGGACC+AGG + chr6.3:42890221-42890240 MS.gene059382:CDS 55.0%
GTGGGGGAATCATGTTCGCG+TGG + chr6.3:42890435-42890454 MS.gene059382:CDS 60.0%
!! CTAAAGGGCGCTGACCTGAG+CGG - chr6.3:42890772-42890791 None:intergenic 60.0%
GGATCAGGGAGAGTCCGCTC+AGG + chr6.3:42890755-42890774 MS.gene059382:CDS 65.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 42890167 42890823 42890167 ID=MS.gene059382
chr6.3 mRNA 42890167 42890823 42890167 ID=MS.gene059382.t1;Parent=MS.gene059382
chr6.3 exon 42890167 42890823 42890167 ID=MS.gene059382.t1.exon1;Parent=MS.gene059382.t1
chr6.3 CDS 42890167 42890823 42890167 ID=cds.MS.gene059382.t1;Parent=MS.gene059382.t1
Gene Sequence

>MS.gene059382

ATGAATATTTTGGGTTTGAATGTCACACGGATTACTTGGTTTGGAAACAGGTTGGGGTTGATTCGGTTAGGACCAGGGGAAAGTATTACTCAGTCAGGACCATCACAGTCCATACGCCCGTTTGTTCTTAGGTATAATCCAGTTGTGATGGGATTGGTGGATACAAGACGTTCGAAGCTCACTGAGGAAACGGTTAAAGGATGTTTGAAGAAGGAAGACAATTTAAGCTGGGTAGATGTTCCAAAATCATTTTTCAAAGACAAGAAAAGTGGGGGAATCATGTTCGCGTGGAATGAACTTTATTTTACGATATCAAAAACTTTACCAAATAAGAGATGGCTCTTGGTTGAAGGTAATTTGATAAAAGAACGTATTGATGTGTCTTTTCTTTTGGTTTCTGAACCAAGTTGTCGTAAAGAGAGAGATAATTTACGGAGTGATTTGCTGAACTTAAGGAAGACAATTTCAAATCCTTTGGTGGTGATGGGTAAGTTTTATGATGGTGTACCATCCGAAGAAAAAGAAGCCGATTTTGAGTATTTTGTAGACCATAAATTTTCAAAGCATTTTAAGGTTCGGAAATGGTTGGGATCAGGGAGAGTCCGCTCAGGTCAGCGCCCTTTAGTGCTTCATTGTTATGAACAAGATGATATTTGA

Protein sequence

>MS.gene059382.t1

MNILGLNVTRITWFGNRLGLIRLGPGESITQSGPSQSIRPFVLRYNPVVMGLVDTRRSKLTEETVKGCLKKEDNLSWVDVPKSFFKDKKSGGIMFAWNELYFTISKTLPNKRWLLVEGNLIKERIDVSFLLVSEPSCRKERDNLRSDLLNLRKTISNPLVVMGKFYDGVPSEEKEADFEYFVDHKFSKHFKVRKWLGSGRVRSGQRPLVLHCYEQDDI