AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049669


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049669.t1 MTR_3g064240 98.780 82 1 0 1 82 1 82 8.95e-55 164
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049669.t1 AT4G21192 75.000 80 20 0 1 80 1 80 3.25e-42 132
MS.gene049669.t1 AT4G21192 75.000 80 20 0 1 80 1 80 3.25e-42 132

Find 9 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCCTATTGCAAAATTCTT+TGG 0.235808 3.3:+57809749 MS.gene049669:CDS
TACAGAATTGAAAATAAAAT+TGG 0.247760 3.3:+57809778 MS.gene049669:CDS
GGATGTTTGTGCAATGTTAA+AGG 0.281917 3.3:-57809044 None:intergenic
GTGCTTAATGCAGATTATTG+AGG 0.522954 3.3:+57809703 MS.gene049669:intron
ACAGAAAGCTGTGAAGAGAA+AGG 0.562696 3.3:+57809936 MS.gene049669:intron
TGAAAAGAGCAAAGAACTCA+AGG 0.575643 3.3:+57809966 MS.gene049669:CDS
AAACTCACTTCAGCGCACAT+AGG 0.624864 3.3:-57809065 None:intergenic
GGAGTTTCAGAAGTGTCATG+TGG 0.645843 3.3:+57809724 MS.gene049669:CDS
TGTTTGTGCAATGTTAAAGG+AGG 0.660285 3.3:-57809041 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAACATTTTAAAAAAAAG+AGG - chr3.3:57809098-57809117 None:intergenic 10.0%
!! GAACTTTAACATAAAAAATA+AGG - chr3.3:57809857-57809876 None:intergenic 15.0%
!! TACAGAATTGAAAATAAAAT+TGG + chr3.3:57809778-57809797 MS.gene049669:CDS 15.0%
!! TTATATTGGAATAATGAAAT+GGG + chr3.3:57809261-57809280 MS.gene049669:intron 15.0%
!! CATTTCATTATTCCAATATA+AGG - chr3.3:57809262-57809281 None:intergenic 20.0%
!! CTTATATTGGAATAATGAAA+TGG + chr3.3:57809260-57809279 MS.gene049669:intron 20.0%
!! TCATAAACAAAACCTTATAT+TGG + chr3.3:57809247-57809266 MS.gene049669:intron 20.0%
!!! AATTGATTCTAAATCCATTA+TGG - chr3.3:57809583-57809602 None:intergenic 20.0%
!!! GATAATTTATCTTTTTCCTT+TGG + chr3.3:57809629-57809648 MS.gene049669:intron 20.0%
! ATTATGGAGTATGAAAGTAA+GGG - chr3.3:57809567-57809586 None:intergenic 25.0%
! GAAATTAGTAGTTGAAAGTT+TGG + chr3.3:57809224-57809243 MS.gene049669:intron 25.0%
AGATGAAAATTACTTACCAC+AGG - chr3.3:57809906-57809925 None:intergenic 30.0%
ATTTCAATTATCCCATGACA+TGG - chr3.3:57809209-57809228 None:intergenic 30.0%
CATCCTATTGCAAAATTCTT+TGG + chr3.3:57809749-57809768 MS.gene049669:CDS 30.0%
CATTATGGAGTATGAAAGTA+AGG - chr3.3:57809568-57809587 None:intergenic 30.0%
CTTACTTTCATACTCCATAA+TGG + chr3.3:57809566-57809585 MS.gene049669:intron 30.0%
GAATAATGAAATGGGACTTA+GGG + chr3.3:57809269-57809288 MS.gene049669:intron 30.0%
! TTCTTTGCTCTTTTCAAAGT+TGG - chr3.3:57809962-57809981 None:intergenic 30.0%
ACCAAGCTGTTTACCAAAAT+AGG + chr3.3:57809483-57809502 MS.gene049669:intron 35.0%
ATAGCACTTGTGACATTATC+TGG + chr3.3:57809329-57809348 MS.gene049669:intron 35.0%
ATATTTCCATGTCCATGTCA+TGG + chr3.3:57809194-57809213 MS.gene049669:intron 35.0%
CCAAGCTGTTTACCAAAATA+GGG + chr3.3:57809484-57809503 MS.gene049669:intron 35.0%
GGAATAATGAAATGGGACTT+AGG + chr3.3:57809268-57809287 MS.gene049669:intron 35.0%
GGATGTTTGTGCAATGTTAA+AGG - chr3.3:57809047-57809066 None:intergenic 35.0%
GTGCTTAATGCAGATTATTG+AGG + chr3.3:57809703-57809722 MS.gene049669:intron 35.0%
TAGCACTTGTGACATTATCT+GGG + chr3.3:57809330-57809349 MS.gene049669:intron 35.0%
TATTTCCATGTCCATGTCAT+GGG + chr3.3:57809195-57809214 MS.gene049669:intron 35.0%
TGAAAAGAGCAAAGAACTCA+AGG + chr3.3:57809966-57809985 MS.gene049669:CDS 35.0%
TGTTTGTGCAATGTTAAAGG+AGG - chr3.3:57809044-57809063 None:intergenic 35.0%
! AGGAACAATTGCGAGTTTTA+AGG + chr3.3:57809986-57810005 MS.gene049669:CDS 35.0%
! TCGCCAAAGAATTTTGCAAT+AGG - chr3.3:57809755-57809774 None:intergenic 35.0%
!! CTTTGCTTTGAAGTTTGATC+TGG + chr3.3:57809876-57809895 MS.gene049669:intron 35.0%
ACAGAAAGCTGTGAAGAGAA+AGG + chr3.3:57809936-57809955 MS.gene049669:intron 40.0%
ATTATCCCATGACATGGACA+TGG - chr3.3:57809203-57809222 None:intergenic 40.0%
CAATACCTCTTAGTGACTTG+AGG - chr3.3:57809510-57809529 None:intergenic 40.0%
CTGATAACTTTCAGCACCAA+AGG - chr3.3:57809648-57809667 None:intergenic 40.0%
TAAGGGAATGCCAATTGACA+GGG - chr3.3:57809550-57809569 None:intergenic 40.0%
TTATCTGTTGCCCTGTCAAT+TGG + chr3.3:57809537-57809556 MS.gene049669:intron 40.0%
TTCTCTTCACAGCTTTCTGT+TGG - chr3.3:57809936-57809955 None:intergenic 40.0%
!! CTTTGGTGCTGAAAGTTATC+AGG + chr3.3:57809646-57809665 MS.gene049669:intron 40.0%
!! TCACTAAGAGGTATTGCCAA+GGG + chr3.3:57809514-57809533 MS.gene049669:intron 40.0%
!!! AGTTTGATCTGGTTTTCCTG+TGG + chr3.3:57809887-57809906 MS.gene049669:intron 40.0%
!!! CCCTATTTTGGTAAACAGCT+TGG - chr3.3:57809487-57809506 None:intergenic 40.0%
AAACTCACTTCAGCGCACAT+AGG - chr3.3:57809068-57809087 None:intergenic 45.0%
GTAAGGGAATGCCAATTGAC+AGG - chr3.3:57809551-57809570 None:intergenic 45.0%
! AGTGACTTGAGGCCCTATTT+TGG - chr3.3:57809499-57809518 None:intergenic 45.0%
!! GGAGTTTCAGAAGTGTCATG+TGG + chr3.3:57809724-57809743 MS.gene049669:CDS 45.0%
!! GTCACTAAGAGGTATTGCCA+AGG + chr3.3:57809513-57809532 MS.gene049669:intron 45.0%
ACAGGGCAACAGATAACCCT+TGG - chr3.3:57809533-57809552 None:intergenic 50.0%
TAGGGCCTCAAGTCACTAAG+AGG + chr3.3:57809502-57809521 MS.gene049669:intron 50.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 57809035 57810038 57809035 ID=MS.gene049669
chr3.3 mRNA 57809035 57810038 57809035 ID=MS.gene049669.t1;Parent=MS.gene049669
chr3.3 exon 57809035 57809079 57809035 ID=MS.gene049669.t1.exon1;Parent=MS.gene049669.t1
chr3.3 CDS 57809035 57809079 57809035 ID=cds.MS.gene049669.t1;Parent=MS.gene049669.t1
chr3.3 exon 57809716 57809820 57809716 ID=MS.gene049669.t1.exon2;Parent=MS.gene049669.t1
chr3.3 CDS 57809716 57809820 57809716 ID=cds.MS.gene049669.t1;Parent=MS.gene049669.t1
chr3.3 exon 57809940 57810038 57809940 ID=MS.gene049669.t1.exon3;Parent=MS.gene049669.t1
chr3.3 CDS 57809940 57810038 57809940 ID=cds.MS.gene049669.t1;Parent=MS.gene049669.t1
Gene Sequence

>MS.gene049669

ATGCATCCTCCTTTAACATTGCACAAACATCCTATGTGCGCTGAAGTGAGTTTGTTTTATCCTCTTTTTTTTAAAATGTTTAATTATTTGCTATTAGAATTACTCATGTTCTTATATGTTATTCTTAATAATGTGTTTGTCGCTTATGATCAATTTGATATATTTCCATGTCCATGTCATGGGATAATTGAAATTAGTAGTTGAAAGTTTGGTCATAAACAAAACCTTATATTGGAATAATGAAATGGGACTTAGGGTCTATTTGAGTTGACTTATTTGAGCTTATCTACTAATATAGCACTTGTGACATTATCTGGGAGAGCTTATGATATATTCATAAGTTCTCTACAATAGCTTATAACTTGTACGAAAACAATTTGACTTTATTTTTATTTTTTTGTTATACATAAGAGCTTATATTGATAAGTTCTTATACTATAAGCTGCAAACCAAGCTGTTTACCAAAATAGGGCCTCAAGTCACTAAGAGGTATTGCCAAGGGTTATCTGTTGCCCTGTCAATTGGCATTCCCTTACTTTCATACTCCATAATGGATTTAGAATCAATTTGTGAATTTACTAATTTAGAAGTTTAGATAATTTATCTTTTTCCTTTGGTGCTGAAAGTTATCAGGTATCTTCTTGCAATAGCATACTTTAATTCATGTTGTGCTTAATGCAGATTATTGAGGAGTTTCAGAAGTGTCATGTGGAACATCCTATTGCAAAATTCTTTGGCGAATGTACAGAATTGAAAATAAAATTGGATCGCTGCTTCAGAGCAGAAGTGAGATTTTCAATTTTTCTTTTATTTTCTTTTCCTTATTTTTTATGTTAAAGTTCTTTGCTTTGAAGTTTGATCTGGTTTTCCTGTGGTAAGTAATTTTCATCTATATGTTCCAACAGAAAGCTGTGAAGAGAAAGGCCAACTTTGAAAAGAGCAAAGAACTCAAGGAACAATTGCGAGTTTTAAGGAAAGAAAATGCTGCAAGCAGCAGTCAATAG

Protein sequence

>MS.gene049669.t1

MHPPLTLHKHPMCAEIIEEFQKCHVEHPIAKFFGECTELKIKLDRCFRAEKAVKRKANFEKSKELKEQLRVLRKENAASSSQ