Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56243.t1 | XP_003598238.1 | 93.5 | 186 | 12 | 0 | 1 | 186 | 1 | 186 | 2.20E-90 | 341.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56243.t1 | P17407 | 51.4 | 179 | 85 | 2 | 10 | 186 | 13 | 191 | 9.8e-47 | 188.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56243.t1 | G7IYJ2 | 93.5 | 186 | 12 | 0 | 1 | 186 | 1 | 186 | 1.6e-90 | 341.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene045671 | MS.gene56243 | PPI |
| MS.gene77725 | MS.gene56243 | PPI |
| MS.gene78072 | MS.gene56243 | PPI |
| MS.gene72493 | MS.gene56243 | PPI |
| MS.gene77727 | MS.gene56243 | PPI |
| MS.gene72495 | MS.gene56243 | PPI |
| MS.gene045669 | MS.gene56243 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56243.t1 | MTR_3g008820 | 93.548 | 186 | 12 | 0 | 1 | 186 | 1 | 186 | 3.86e-124 | 347 |
| MS.gene56243.t1 | MTR_3g008840 | 88.372 | 172 | 20 | 0 | 15 | 186 | 16 | 187 | 7.37e-116 | 327 |
| MS.gene56243.t1 | MTR_5g012000 | 47.283 | 184 | 95 | 2 | 5 | 186 | 28 | 211 | 2.42e-62 | 192 |
| MS.gene56243.t1 | MTR_4g101760 | 45.550 | 191 | 95 | 2 | 5 | 186 | 12 | 202 | 1.61e-56 | 177 |
| MS.gene56243.t1 | MTR_4g035905 | 47.879 | 165 | 84 | 1 | 22 | 186 | 34 | 196 | 4.38e-49 | 158 |
| MS.gene56243.t1 | MTR_4g035870 | 45.399 | 163 | 87 | 1 | 24 | 186 | 36 | 196 | 9.31e-44 | 144 |
| MS.gene56243.t1 | MTR_4g035880 | 44.785 | 163 | 88 | 1 | 24 | 186 | 36 | 196 | 1.17e-42 | 141 |
| MS.gene56243.t1 | MTR_4g025550 | 41.270 | 189 | 102 | 5 | 6 | 186 | 14 | 201 | 1.10e-40 | 137 |
| MS.gene56243.t1 | MTR_4g025610 | 39.896 | 193 | 102 | 6 | 7 | 186 | 10 | 201 | 1.11e-38 | 132 |
| MS.gene56243.t1 | MTR_4g025590 | 41.520 | 171 | 91 | 5 | 24 | 186 | 3 | 172 | 1.78e-37 | 128 |
| MS.gene56243.t1 | MTR_4g025620 | 39.683 | 189 | 105 | 5 | 6 | 186 | 14 | 201 | 2.35e-37 | 128 |
| MS.gene56243.t1 | MTR_4g120825 | 36.224 | 196 | 103 | 5 | 8 | 183 | 9 | 202 | 1.37e-35 | 124 |
| MS.gene56243.t1 | MTR_4g120830 | 36.224 | 196 | 103 | 5 | 8 | 183 | 9 | 202 | 1.78e-35 | 123 |
| MS.gene56243.t1 | MTR_8g104765 | 37.500 | 176 | 98 | 4 | 18 | 183 | 38 | 211 | 7.20e-35 | 122 |
| MS.gene56243.t1 | MTR_3g008760 | 35.714 | 182 | 103 | 4 | 12 | 181 | 45 | 224 | 2.84e-31 | 113 |
| MS.gene56243.t1 | MTR_2g043560 | 36.723 | 177 | 96 | 4 | 12 | 183 | 22 | 187 | 3.73e-30 | 110 |
| MS.gene56243.t1 | MTR_7g108790 | 32.727 | 165 | 107 | 3 | 17 | 181 | 26 | 186 | 5.76e-28 | 103 |
| MS.gene56243.t1 | MTR_4g124855 | 33.511 | 188 | 111 | 6 | 5 | 183 | 15 | 197 | 2.93e-24 | 94.7 |
| MS.gene56243.t1 | MTR_1g088540 | 31.507 | 146 | 96 | 2 | 39 | 184 | 1 | 142 | 1.89e-22 | 88.6 |
| MS.gene56243.t1 | MTR_4g035855 | 31.098 | 164 | 105 | 4 | 24 | 183 | 43 | 202 | 1.34e-18 | 79.7 |
| MS.gene56243.t1 | MTR_1g008140 | 29.444 | 180 | 108 | 5 | 19 | 185 | 76 | 249 | 6.40e-15 | 72.4 |
| MS.gene56243.t1 | MTR_8g023310 | 30.675 | 163 | 96 | 6 | 25 | 178 | 68 | 222 | 6.13e-14 | 69.3 |
| MS.gene56243.t1 | MTR_8g023150 | 29.518 | 166 | 106 | 5 | 20 | 181 | 72 | 230 | 1.43e-13 | 68.6 |
| MS.gene56243.t1 | MTR_3g091380 | 28.743 | 167 | 113 | 3 | 21 | 183 | 74 | 238 | 7.73e-13 | 66.2 |
| MS.gene56243.t1 | MTR_4g005170 | 25.000 | 160 | 114 | 4 | 25 | 183 | 63 | 217 | 2.93e-11 | 61.6 |
| MS.gene56243.t1 | MTR_0036s0140 | 29.560 | 159 | 97 | 6 | 23 | 179 | 76 | 221 | 9.86e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56243.t1 | AT5G62350 | 54.070 | 172 | 77 | 2 | 17 | 186 | 31 | 202 | 5.19e-65 | 199 |
| MS.gene56243.t1 | AT4G25260 | 47.264 | 201 | 91 | 2 | 1 | 186 | 1 | 201 | 4.01e-61 | 189 |
| MS.gene56243.t1 | AT3G47380 | 46.040 | 202 | 93 | 4 | 1 | 186 | 1 | 202 | 5.54e-60 | 186 |
| MS.gene56243.t1 | AT1G62770 | 47.340 | 188 | 90 | 4 | 5 | 183 | 9 | 196 | 1.35e-53 | 169 |
| MS.gene56243.t1 | AT4G12390 | 47.305 | 167 | 85 | 1 | 23 | 186 | 38 | 204 | 6.33e-49 | 158 |
| MS.gene56243.t1 | AT5G62360 | 43.293 | 164 | 90 | 2 | 23 | 186 | 42 | 202 | 1.72e-48 | 157 |
| MS.gene56243.t1 | AT5G20740 | 44.444 | 162 | 86 | 2 | 23 | 181 | 32 | 192 | 1.36e-41 | 139 |
| MS.gene56243.t1 | AT5G20740 | 44.444 | 162 | 86 | 2 | 23 | 181 | 66 | 226 | 3.45e-41 | 139 |
| MS.gene56243.t1 | AT1G14890 | 36.923 | 195 | 102 | 3 | 7 | 185 | 28 | 217 | 3.33e-39 | 133 |
| MS.gene56243.t1 | AT4G00080 | 37.563 | 197 | 105 | 8 | 2 | 181 | 3 | 198 | 5.63e-37 | 127 |
| MS.gene56243.t1 | AT1G62760 | 42.857 | 168 | 89 | 3 | 20 | 184 | 143 | 306 | 5.27e-36 | 127 |
| MS.gene56243.t1 | AT2G01610 | 35.938 | 192 | 105 | 4 | 10 | 185 | 30 | 219 | 5.72e-36 | 125 |
| MS.gene56243.t1 | AT4G25250 | 34.568 | 162 | 105 | 1 | 22 | 182 | 35 | 196 | 3.05e-34 | 120 |
| MS.gene56243.t1 | AT1G70720 | 36.598 | 194 | 108 | 5 | 1 | 183 | 5 | 194 | 9.60e-34 | 119 |
| MS.gene56243.t1 | AT1G70720 | 36.598 | 194 | 108 | 5 | 1 | 183 | 17 | 206 | 1.43e-33 | 119 |
| MS.gene56243.t1 | AT1G23205 | 36.170 | 188 | 102 | 5 | 6 | 181 | 17 | 198 | 4.61e-30 | 109 |
| MS.gene56243.t1 | AT5G51520 | 35.366 | 164 | 97 | 3 | 24 | 179 | 37 | 199 | 1.02e-27 | 103 |
| MS.gene56243.t1 | AT3G62820 | 26.702 | 191 | 131 | 5 | 1 | 185 | 5 | 192 | 2.83e-20 | 84.0 |
| MS.gene56243.t1 | AT3G14310 | 32.911 | 158 | 100 | 4 | 25 | 179 | 59 | 213 | 1.24e-19 | 85.9 |
| MS.gene56243.t1 | AT3G49220 | 32.298 | 161 | 99 | 4 | 21 | 180 | 128 | 279 | 4.25e-19 | 84.0 |
| MS.gene56243.t1 | AT3G49220 | 32.500 | 160 | 98 | 4 | 21 | 179 | 128 | 278 | 7.03e-19 | 84.0 |
| MS.gene56243.t1 | AT5G53370 | 31.288 | 163 | 105 | 4 | 18 | 179 | 104 | 260 | 8.21e-19 | 83.6 |
| MS.gene56243.t1 | AT3G49220 | 32.500 | 160 | 98 | 4 | 21 | 179 | 83 | 233 | 8.28e-19 | 83.6 |
| MS.gene56243.t1 | AT5G53370 | 31.288 | 163 | 105 | 4 | 18 | 179 | 68 | 224 | 9.79e-19 | 83.6 |
| MS.gene56243.t1 | AT5G62340 | 28.922 | 204 | 111 | 8 | 6 | 186 | 14 | 206 | 2.39e-17 | 76.6 |
| MS.gene56243.t1 | AT2G47670 | 27.500 | 160 | 113 | 2 | 24 | 183 | 48 | 204 | 4.83e-17 | 75.9 |
| MS.gene56243.t1 | AT1G53830 | 27.607 | 163 | 116 | 2 | 18 | 179 | 55 | 216 | 5.62e-16 | 75.5 |
| MS.gene56243.t1 | AT3G47670 | 28.718 | 195 | 112 | 8 | 5 | 181 | 82 | 267 | 5.04e-13 | 65.9 |
| MS.gene56243.t1 | AT3G14300 | 25.698 | 179 | 123 | 4 | 12 | 182 | 258 | 434 | 4.35e-12 | 64.3 |
| MS.gene56243.t1 | AT3G14300 | 28.402 | 169 | 109 | 5 | 20 | 181 | 454 | 617 | 5.41e-12 | 63.9 |
Find 32 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTCACTCATTAATCAATA+TGG | 0.298645 | 3.3:-4433485 | MS.gene56243:CDS |
| CCAACAAGACCCTCATAAAT+TGG | 0.341835 | 3.3:-4433864 | MS.gene56243:CDS |
| CCAATTTATGAGGGTCTTGT+TGG | 0.370214 | 3.3:+4433864 | None:intergenic |
| GGCTAATTGATAAAGCTGTT+TGG | 0.400503 | 3.3:+4433840 | None:intergenic |
| TAGGCTTAGCCGTTCACTTA+AGG | 0.410233 | 3.3:-4433699 | MS.gene56243:CDS |
| GAGGATTTGATGAAATTTGT+TGG | 0.419280 | 3.3:+4433950 | None:intergenic |
| TCAATGGTCAAGATTTCAAT+TGG | 0.421550 | 3.3:-4433658 | MS.gene56243:CDS |
| GAGTTGAAGTTGCAATAGAT+AGG | 0.430516 | 3.3:+4433972 | None:intergenic |
| AAATTACTGATAGTGTTGAT+AGG | 0.448838 | 3.3:-4433718 | MS.gene56243:CDS |
| CTTAAACTTTGCAAGATCAA+TGG | 0.453176 | 3.3:-4433674 | MS.gene56243:CDS |
| TTTGGTGGAAAAGCACTTGA+TGG | 0.453873 | 3.3:-4433569 | MS.gene56243:CDS |
| AACAAAACTCAATCCACAAA+AGG | 0.469923 | 3.3:-4433815 | MS.gene56243:CDS |
| TGAATAGCAGCATATTCTCT+TGG | 0.472801 | 3.3:+4433755 | None:intergenic |
| AACTTCTATTAGATCAAGAA+TGG | 0.475846 | 3.3:-4433537 | MS.gene56243:CDS |
| TTGAAGTTGCAATAGATAGG+AGG | 0.478728 | 3.3:+4433975 | None:intergenic |
| AGTACTTGTAGTGATGGATT+TGG | 0.488408 | 3.3:-4433587 | MS.gene56243:CDS |
| GCTGTTTGGACCAATTTATG+AGG | 0.493258 | 3.3:+4433854 | None:intergenic |
| GAGATTCAACACAAAGTGTT+GGG | 0.500666 | 3.3:+4433909 | None:intergenic |
| AAGAAGGACATTTGTTGCCA+TGG | 0.510677 | 3.3:+4434012 | None:intergenic |
| TGAAGTTGCAATAGATAGGA+GGG | 0.517546 | 3.3:+4433976 | None:intergenic |
| GTTTAAGCTCCTTAAGTGAA+CGG | 0.545857 | 3.3:+4433690 | None:intergenic |
| AGAGATTCAACACAAAGTGT+TGG | 0.548978 | 3.3:+4433908 | None:intergenic |
| ACTTGTAGTGATGGATTTGG+TGG | 0.550252 | 3.3:-4433584 | MS.gene56243:CDS |
| GGGTTAAGAGAAGTAAAAGA+AGG | 0.553192 | 3.3:+4433996 | None:intergenic |
| TCAATATGGTACTAATCACT+AGG | 0.554200 | 3.3:-4433471 | None:intergenic |
| CTGTTTGGACCAATTTATGA+GGG | 0.561937 | 3.3:+4433855 | None:intergenic |
| ATATAAGTAATGTTCAAACA+TGG | 0.577147 | 3.3:-4433634 | MS.gene56243:CDS |
| GATGCAAGTACTTGTAGTGA+TGG | 0.592136 | 3.3:-4433593 | MS.gene56243:CDS |
| CAATATGGTACTAATCACTA+GGG | 0.596788 | 3.3:-4433470 | None:intergenic |
| TATAAGTAATGTTCAAACAT+GGG | 0.606584 | 3.3:-4433633 | MS.gene56243:CDS |
| GGTGGAAAAGCACTTGATGG+AGG | 0.622229 | 3.3:-4433566 | MS.gene56243:CDS |
| GTATGATGTTGTGCTACATG+AGG | 0.681149 | 3.3:+4433931 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAATGTGCAAAAATTATAA+AGG | - | chr3.3:4433697-4433716 | MS.gene56243:CDS | 20.0% |
| !! | ATATAAGTAATGTTCAAACA+TGG | - | chr3.3:4433848-4433867 | MS.gene56243:CDS | 20.0% |
| !! | TATAAGTAATGTTCAAACAT+GGG | - | chr3.3:4433849-4433868 | MS.gene56243:CDS | 20.0% |
| ! | AACTTCTATTAGATCAAGAA+TGG | - | chr3.3:4433945-4433964 | MS.gene56243:CDS | 25.0% |
| ! | CTTTCACTCATTAATCAATA+TGG | - | chr3.3:4433997-4434016 | MS.gene56243:CDS | 25.0% |
| !!! | AAATTACTGATAGTGTTGAT+AGG | - | chr3.3:4433764-4433783 | MS.gene56243:CDS | 25.0% |
| AACAAAACTCAATCCACAAA+AGG | - | chr3.3:4433667-4433686 | MS.gene56243:CDS | 30.0% | |
| CTTAAACTTTGCAAGATCAA+TGG | - | chr3.3:4433808-4433827 | MS.gene56243:CDS | 30.0% | |
| GAGGATTTGATGAAATTTGT+TGG | + | chr3.3:4433535-4433554 | None:intergenic | 30.0% | |
| TCAATGGTCAAGATTTCAAT+TGG | - | chr3.3:4433824-4433843 | MS.gene56243:CDS | 30.0% | |
| !!! | TTGTGGATTGAGTTTTGTTT+AGG | + | chr3.3:4433666-4433685 | None:intergenic | 30.0% |
| AGAGATTCAACACAAAGTGT+TGG | + | chr3.3:4433577-4433596 | None:intergenic | 35.0% | |
| CTGTTTGGACCAATTTATGA+GGG | + | chr3.3:4433630-4433649 | None:intergenic | 35.0% | |
| GAGTTGAAGTTGCAATAGAT+AGG | + | chr3.3:4433513-4433532 | None:intergenic | 35.0% | |
| GGCTAATTGATAAAGCTGTT+TGG | + | chr3.3:4433645-4433664 | None:intergenic | 35.0% | |
| GGGTTAAGAGAAGTAAAAGA+AGG | + | chr3.3:4433489-4433508 | None:intergenic | 35.0% | |
| GTTTAAGCTCCTTAAGTGAA+CGG | + | chr3.3:4433795-4433814 | None:intergenic | 35.0% | |
| TGAAGTTGCAATAGATAGGA+GGG | + | chr3.3:4433509-4433528 | None:intergenic | 35.0% | |
| TGAATAGCAGCATATTCTCT+TGG | + | chr3.3:4433730-4433749 | None:intergenic | 35.0% | |
| TTGAAGTTGCAATAGATAGG+AGG | + | chr3.3:4433510-4433529 | None:intergenic | 35.0% | |
| ! | CATTGTAACATAGCCTTTTG+TGG | + | chr3.3:4433683-4433702 | None:intergenic | 35.0% |
| ! | GAGATTCAACACAAAGTGTT+GGG | + | chr3.3:4433576-4433595 | None:intergenic | 35.0% |
| !! | AGTACTTGTAGTGATGGATT+TGG | - | chr3.3:4433895-4433914 | MS.gene56243:CDS | 35.0% |
| CCAACAAGACCCTCATAAAT+TGG | - | chr3.3:4433618-4433637 | MS.gene56243:CDS | 40.0% | |
| GATGCAAGTACTTGTAGTGA+TGG | - | chr3.3:4433889-4433908 | MS.gene56243:CDS | 40.0% | |
| GCTGTTTGGACCAATTTATG+AGG | + | chr3.3:4433631-4433650 | None:intergenic | 40.0% | |
| GTATGATGTTGTGCTACATG+AGG | + | chr3.3:4433554-4433573 | None:intergenic | 40.0% | |
| ! | CCAATTTATGAGGGTCTTGT+TGG | + | chr3.3:4433621-4433640 | None:intergenic | 40.0% |
| !! | ACTTGTAGTGATGGATTTGG+TGG | - | chr3.3:4433898-4433917 | MS.gene56243:CDS | 40.0% |
| !! | TTTGGTGGAAAAGCACTTGA+TGG | - | chr3.3:4433913-4433932 | MS.gene56243:CDS | 40.0% |
| TAGGCTTAGCCGTTCACTTA+AGG | - | chr3.3:4433783-4433802 | MS.gene56243:CDS | 45.0% | |
| !! | GGTGGAAAAGCACTTGATGG+AGG | - | chr3.3:4433916-4433935 | MS.gene56243:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 4433472 | 4434032 | 4433472 | ID=MS.gene56243 |
| chr3.3 | mRNA | 4433472 | 4434032 | 4433472 | ID=MS.gene56243.t1;Parent=MS.gene56243 |
| chr3.3 | exon | 4433472 | 4434032 | 4433472 | ID=MS.gene56243.t1.exon1;Parent=MS.gene56243.t1 |
| chr3.3 | CDS | 4433472 | 4434032 | 4433472 | ID=cds.MS.gene56243.t1;Parent=MS.gene56243.t1 |
| Gene Sequence |
| Protein sequence |
