AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56438


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56438.t1 MTR_4g083540 96.633 297 6 1 1 297 1 293 0.0 591
MS.gene56438.t1 MTR_2g028830 50.617 324 128 8 1 297 1 319 1.71e-100 297
MS.gene56438.t1 MTR_2g069350 36.240 367 163 11 1 297 1 366 5.32e-60 195
MS.gene56438.t1 MTR_5g013690 32.500 200 127 5 102 294 134 332 1.08e-27 110
MS.gene56438.t1 MTR_5g013690 32.500 200 127 5 102 294 71 269 1.90e-27 107
MS.gene56438.t1 MTR_4g099060 34.300 207 119 5 98 294 84 283 7.45e-26 103
MS.gene56438.t1 MTR_4g099060 34.300 207 119 5 98 294 129 328 1.32e-25 104
MS.gene56438.t1 MTR_4g036485 32.759 174 109 3 112 285 35 200 4.94e-22 92.0
MS.gene56438.t1 MTR_1g019250 29.907 214 132 5 85 284 119 328 5.97e-22 94.4
MS.gene56438.t1 MTR_3g117970 30.270 185 104 4 104 285 22 184 3.10e-17 78.6
MS.gene56438.t1 MTR_4g073070 30.570 193 107 6 109 297 85 254 1.40e-13 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56438.t1 AT5G45100 42.532 308 151 7 1 297 1 293 3.09e-67 212
MS.gene56438.t1 AT4G19700 39.869 306 172 8 1 297 1 303 4.75e-63 201
MS.gene56438.t1 AT5G45100 47.210 233 104 4 76 297 42 266 2.59e-61 196
MS.gene56438.t1 AT3G12920 38.127 299 143 9 25 297 52 334 5.58e-54 179
MS.gene56438.t1 AT1G79110 43.578 218 101 4 102 297 115 332 3.26e-48 164
MS.gene56438.t1 AT1G79110 43.578 218 101 4 102 297 137 354 4.68e-48 164
MS.gene56438.t1 AT1G79110 42.986 221 101 5 102 297 137 357 5.18e-46 159
MS.gene56438.t1 AT1G60610 30.932 236 135 4 73 294 116 337 6.72e-25 102
MS.gene56438.t1 AT1G60610 30.932 236 135 4 73 294 116 337 6.72e-25 102
MS.gene56438.t1 AT1G60610 30.932 236 135 4 73 294 116 337 6.72e-25 102
MS.gene56438.t1 AT1G60610 30.932 236 135 4 73 294 116 337 6.72e-25 102
MS.gene56438.t1 AT1G60610 30.932 236 135 4 73 294 116 337 6.72e-25 102
MS.gene56438.t1 AT1G45976 27.982 218 128 4 85 286 111 315 1.65e-23 98.6
MS.gene56438.t1 AT1G10650 30.374 214 127 4 97 294 73 280 4.82e-23 96.3
MS.gene56438.t1 AT1G10650 30.374 214 127 4 97 294 129 336 6.18e-23 97.1
MS.gene56438.t1 AT4G35070 32.240 183 114 3 108 284 74 252 6.77e-21 90.1
MS.gene56438.t1 AT4G35070 32.240 183 114 3 108 284 19 197 1.43e-20 88.2
MS.gene56438.t1 AT1G32740 30.319 188 125 2 111 292 76 263 5.48e-20 87.8
MS.gene56438.t1 AT1G32740 30.319 188 125 2 111 292 76 263 5.48e-20 87.8
MS.gene56438.t1 AT1G32740 30.319 188 125 2 111 292 109 296 5.77e-20 88.2
MS.gene56438.t1 AT1G32740 29.949 197 114 4 111 292 120 307 6.48e-20 88.2
MS.gene56438.t1 AT5G47050 28.643 199 131 2 104 294 102 297 1.67e-19 87.0
MS.gene56438.t1 AT4G17680 26.070 257 152 6 73 294 59 312 1.17e-18 84.7
MS.gene56438.t1 AT4G17680 26.667 195 133 3 107 294 49 240 1.05e-17 80.9
MS.gene56438.t1 AT4G17680 27.869 183 121 4 119 294 10 188 2.59e-13 67.8

Find 55 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGATTTCAGATTGTTGATTT+TGG 0.126414 4.3:-27900745 None:intergenic
GTTTCTTTGTTACTGCTTCT+TGG 0.130913 4.3:-27900976 None:intergenic
GGGAAATTGTGCAGCGGTTG+TGG 0.242036 4.3:+27901268 MS.gene56438:CDS
GTAGAATTGTTGATTGAATT+TGG 0.242276 4.3:-27900477 None:intergenic
CTGAAACGCTTTCTTGAATC+AGG 0.252113 4.3:-27900623 None:intergenic
AAAAGCGAAAACACTGATTA+TGG 0.280960 4.3:+27901057 MS.gene56438:CDS
TGAAAAGGAAAGAACGATTC+AGG 0.297477 4.3:-27900548 None:intergenic
GGAAATTGTGCAGCGGTTGT+GGG 0.352965 4.3:+27901269 MS.gene56438:CDS
GTGACAGTACTATCAGCTTT+TGG 0.356081 4.3:-27900590 None:intergenic
TGATTATGGAGAATCAGATT+TGG 0.382990 4.3:+27901071 MS.gene56438:CDS
CAGAAAGATGGTTGATGAAA+AGG 0.384641 4.3:-27900563 None:intergenic
TAACGAATTAGAACAAGTTC+TGG 0.406835 4.3:+27901138 MS.gene56438:CDS
CATGTGAATAGCACCATTCA+TGG 0.420481 4.3:-27900507 None:intergenic
AGAAGAAGAGATTCAGAGAA+TGG 0.421799 4.3:+27901009 MS.gene56438:CDS
GAATCAGATTTGGAGAGAAA+TGG 0.423763 4.3:+27901081 MS.gene56438:CDS
TGTGCAGCGGTTGTGGGGTA+AGG 0.425056 4.3:+27901275 MS.gene56438:CDS
CACAAACACAAATGCCTACA+TGG 0.434344 4.3:-27901319 None:intergenic
AAGCGTCGTCGGCGGTGGTT+GGG 0.435829 4.3:+27901248 MS.gene56438:CDS
TTCTACAATGCTTCCATGAA+TGG 0.441017 4.3:+27900494 MS.gene56438:CDS
TCTTGAATCAGGGATGTGAT+AGG 0.458364 4.3:-27900612 None:intergenic
GGAAGAAGCGTCGTCGGCGG+TGG 0.464269 4.3:+27901243 MS.gene56438:CDS
ACAGACAGAGAGAGTGAGAA+TGG 0.475032 4.3:+27900904 MS.gene56438:intron
GAAGCGTCGTCGGCGGTGGT+TGG 0.475906 4.3:+27901247 MS.gene56438:CDS
GAAGATGCAGCGTCGAGTTG+TGG 0.486897 4.3:+27901187 MS.gene56438:CDS
CGTGAAGGAGGAGGTGGTGG+TGG 0.493521 4.3:+27901222 MS.gene56438:CDS
GAAAAGGAAAGAACGATTCA+GGG 0.507955 4.3:-27900547 None:intergenic
TCAGGGATTGTTGAAGACAT+AGG 0.522810 4.3:-27900530 None:intergenic
CCTCTCAATTCTCATGGCAG+TGG 0.525047 4.3:+27900306 None:intergenic
CCACGTGAAGGAGGAGGTGG+TGG 0.525931 4.3:+27901219 MS.gene56438:CDS
TTGTGTTTGTGTACAATGTG+TGG 0.529559 4.3:+27901331 MS.gene56438:CDS
ACGCTGCATCTTCATCGTTA+CGG 0.543093 4.3:-27901177 None:intergenic
TAGTGCTGTTATTGCCATGT+AGG 0.545970 4.3:+27901305 MS.gene56438:CDS
GTGCAGCGGTTGTGGGGTAA+GGG 0.555636 4.3:+27901276 MS.gene56438:CDS
TGTGTTTGTGTACAATGTGT+GGG 0.556297 4.3:+27901332 MS.gene56438:CDS
GGAGAGAAGAAATTCATATG+AGG 0.564999 4.3:-27900334 None:intergenic
AAAGAGGGCAATTACGAATG+TGG 0.572431 4.3:-27901357 None:intergenic
CACAGTCACAAATCAATCAC+AGG 0.574904 4.3:+27900356 MS.gene56438:CDS
CCACCACGTGAAGGAGGAGG+TGG 0.585785 4.3:+27901216 MS.gene56438:CDS
GGTGGTGGAAGAAGCGTCGT+CGG 0.587267 4.3:+27901237 MS.gene56438:CDS
AAGAGGGCAATTACGAATGT+GGG 0.595280 4.3:-27901356 None:intergenic
TGAAACGCTTTCTTGAATCA+GGG 0.595468 4.3:-27900622 None:intergenic
GAGAAGAAATTCATATGAGG+AGG 0.604224 4.3:-27900331 None:intergenic
AGATTCTGATCAACAAAGGA+AGG 0.605193 4.3:-27900710 None:intergenic
CCACCACCTCCTCCTTCACG+TGG 0.618649 4.3:-27901219 None:intergenic
GGTGGAAGAAGCGTCGTCGG+CGG 0.623677 4.3:+27901240 MS.gene56438:CDS
GTGGTTGGGAAATTGTGCAG+CGG 0.623860 4.3:+27901262 MS.gene56438:CDS
GCAATCAAGAATGCTACAAG+CGG 0.626043 4.3:+27900949 MS.gene56438:CDS
TAACCACCACGTGAAGGAGG+AGG 0.626115 4.3:+27901213 MS.gene56438:CDS
GAGGAGATTCTGATCAACAA+AGG 0.626764 4.3:-27900714 None:intergenic
CTGTGATTGATTTGTGACTG+TGG 0.627583 4.3:-27900355 None:intergenic
GAAATTGTGCAGCGGTTGTG+GGG 0.636186 4.3:+27901270 MS.gene56438:CDS
CCACCTCCTCCTTCACGTGG+TGG 0.641078 4.3:-27901216 None:intergenic
AAGAGTGTATGATTCTTCAG+TGG 0.660791 4.3:-27900651 None:intergenic
AAGTAACCACCACGTGAAGG+AGG 0.662727 4.3:+27901210 MS.gene56438:CDS
TGGAAGTAACCACCACGTGA+AGG 0.663711 4.3:+27901207 MS.gene56438:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAAAGAAACTGTTAAA+AGG + chr4.3:27900804-27900823 MS.gene56438:intron 20.0%
!! GTATAATATAAGAAAACTGA+AGG - chr4.3:27900412-27900431 None:intergenic 20.0%
! ATATAAGAAAACTGAAGGTA+AGG - chr4.3:27900407-27900426 None:intergenic 25.0%
! TAATTCCAGAAAAACAAAGA+GGG - chr4.3:27901375-27901394 None:intergenic 25.0%
! TTAATTCCAGAAAAACAAAG+AGG - chr4.3:27901376-27901395 None:intergenic 25.0%
!! GTAGAATTGTTGATTGAATT+TGG - chr4.3:27900480-27900499 None:intergenic 25.0%
!! TTGCTTAGATTTTGTGATTA+TGG + chr4.3:27900841-27900860 MS.gene56438:intron 25.0%
TAACGAATTAGAACAAGTTC+TGG + chr4.3:27901138-27901157 MS.gene56438:CDS 30.0%
TGAATTTGGTTATATCTCTG+TGG - chr4.3:27900466-27900485 None:intergenic 30.0%
TGATTATGGAGAATCAGATT+TGG + chr4.3:27901071-27901090 MS.gene56438:CDS 30.0%
! AAAAGCGAAAACACTGATTA+TGG + chr4.3:27901057-27901076 MS.gene56438:CDS 30.0%
! GAGAGTTTGATATTTTTCTG+AGG - chr4.3:27900683-27900702 None:intergenic 30.0%
!! TTGATATTTTTCTGAGGAAG+AGG - chr4.3:27900677-27900696 None:intergenic 30.0%
!!! CGATTTCAGATTGTTGATTT+TGG - chr4.3:27900748-27900767 None:intergenic 30.0%
AGAAGAAGAGATTCAGAGAA+TGG + chr4.3:27901009-27901028 MS.gene56438:CDS 35.0%
AGATTCTGATCAACAAAGGA+AGG - chr4.3:27900713-27900732 None:intergenic 35.0%
ATATCTCTGTGGAAAAACAG+AGG - chr4.3:27900455-27900474 None:intergenic 35.0%
GAAAAGGAAAGAACGATTCA+GGG - chr4.3:27900550-27900569 None:intergenic 35.0%
GAATCAGATTTGGAGAGAAA+TGG + chr4.3:27901081-27901100 MS.gene56438:CDS 35.0%
GAGAAGAAATTCATATGAGG+AGG - chr4.3:27900334-27900353 None:intergenic 35.0%
GGAGAGAAGAAATTCATATG+AGG - chr4.3:27900337-27900356 None:intergenic 35.0%
GTTTCTTTGTTACTGCTTCT+TGG - chr4.3:27900979-27900998 None:intergenic 35.0%
TGAAAAGGAAAGAACGATTC+AGG - chr4.3:27900551-27900570 None:intergenic 35.0%
TGAAACGCTTTCTTGAATCA+GGG - chr4.3:27900625-27900644 None:intergenic 35.0%
TGTGTTTGTGTACAATGTGT+GGG + chr4.3:27901332-27901351 MS.gene56438:CDS 35.0%
TTCTACAATGCTTCCATGAA+TGG + chr4.3:27900494-27900513 MS.gene56438:CDS 35.0%
TTGTGTTTGTGTACAATGTG+TGG + chr4.3:27901331-27901350 MS.gene56438:CDS 35.0%
! AAGAGTGTATGATTCTTCAG+TGG - chr4.3:27900654-27900673 None:intergenic 35.0%
!! CAGAAAGATGGTTGATGAAA+AGG - chr4.3:27900566-27900585 None:intergenic 35.0%
!! GTTGATTTTGGAGATGATAG+AGG - chr4.3:27900736-27900755 None:intergenic 35.0%
!!! AATTGCCCTCTTTGTTTTTC+TGG + chr4.3:27901367-27901386 MS.gene56438:CDS 35.0%
AAAGAGGGCAATTACGAATG+TGG - chr4.3:27901360-27901379 None:intergenic 40.0%
AAGAGGGCAATTACGAATGT+GGG - chr4.3:27901359-27901378 None:intergenic 40.0%
CACAAACACAAATGCCTACA+TGG - chr4.3:27901322-27901341 None:intergenic 40.0%
CACAGTCACAAATCAATCAC+AGG + chr4.3:27900356-27900375 MS.gene56438:CDS 40.0%
CATGTGAATAGCACCATTCA+TGG - chr4.3:27900510-27900529 None:intergenic 40.0%
CTGAAACGCTTTCTTGAATC+AGG - chr4.3:27900626-27900645 None:intergenic 40.0%
GAGGAGATTCTGATCAACAA+AGG - chr4.3:27900717-27900736 None:intergenic 40.0%
GCAATCAAGAATGCTACAAG+CGG + chr4.3:27900949-27900968 MS.gene56438:CDS 40.0%
TCAGGGATTGTTGAAGACAT+AGG - chr4.3:27900533-27900552 None:intergenic 40.0%
TCTTGAATCAGGGATGTGAT+AGG - chr4.3:27900615-27900634 None:intergenic 40.0%
! GTGACAGTACTATCAGCTTT+TGG - chr4.3:27900593-27900612 None:intergenic 40.0%
! TAGTGCTGTTATTGCCATGT+AGG + chr4.3:27901305-27901324 MS.gene56438:CDS 40.0%
!! CTGTGATTGATTTGTGACTG+TGG - chr4.3:27900358-27900377 None:intergenic 40.0%
!!! GCTTTTGGTTCACAGAAAGA+TGG - chr4.3:27900578-27900597 None:intergenic 40.0%
ACAGACAGAGAGAGTGAGAA+TGG + chr4.3:27900904-27900923 MS.gene56438:intron 45.0%
ACGCTGCATCTTCATCGTTA+CGG - chr4.3:27901180-27901199 None:intergenic 45.0%
AAGTAACCACCACGTGAAGG+AGG + chr4.3:27901210-27901229 MS.gene56438:CDS 50.0%
GAAATTGTGCAGCGGTTGTG+GGG + chr4.3:27901270-27901289 MS.gene56438:CDS 50.0%
GGAAATTGTGCAGCGGTTGT+GGG + chr4.3:27901269-27901288 MS.gene56438:CDS 50.0%
GTGGTTGGGAAATTGTGCAG+CGG + chr4.3:27901262-27901281 MS.gene56438:CDS 50.0%
TGGAAGTAACCACCACGTGA+AGG + chr4.3:27901207-27901226 MS.gene56438:CDS 50.0%
GGGAAATTGTGCAGCGGTTG+TGG + chr4.3:27901268-27901287 MS.gene56438:CDS 55.0%
TAACCACCACGTGAAGGAGG+AGG + chr4.3:27901213-27901232 MS.gene56438:CDS 55.0%
! GAAGATGCAGCGTCGAGTTG+TGG + chr4.3:27901187-27901206 MS.gene56438:CDS 55.0%
GTGCAGCGGTTGTGGGGTAA+GGG + chr4.3:27901276-27901295 MS.gene56438:CDS 60.0%
TGTGCAGCGGTTGTGGGGTA+AGG + chr4.3:27901275-27901294 MS.gene56438:CDS 60.0%
!! GGTGGTGGAAGAAGCGTCGT+CGG + chr4.3:27901237-27901256 MS.gene56438:CDS 60.0%
CCACCACCTCCTCCTTCACG+TGG - chr4.3:27901222-27901241 None:intergenic 65.0%
CCACCACGTGAAGGAGGAGG+TGG + chr4.3:27901216-27901235 MS.gene56438:CDS 65.0%
CCACCTCCTCCTTCACGTGG+TGG - chr4.3:27901219-27901238 None:intergenic 65.0%
CCACGTGAAGGAGGAGGTGG+TGG + chr4.3:27901219-27901238 MS.gene56438:CDS 65.0%
! AAGCGTCGTCGGCGGTGGTT+GGG + chr4.3:27901248-27901267 MS.gene56438:CDS 65.0%
! CGTGAAGGAGGAGGTGGTGG+TGG + chr4.3:27901222-27901241 MS.gene56438:CDS 65.0%
!! GGTGGAAGAAGCGTCGTCGG+CGG + chr4.3:27901240-27901259 MS.gene56438:CDS 65.0%
!! GAAGCGTCGTCGGCGGTGGT+TGG + chr4.3:27901247-27901266 MS.gene56438:CDS 70.0%
!! GGAAGAAGCGTCGTCGGCGG+TGG + chr4.3:27901243-27901262 MS.gene56438:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 27900319 27901423 27900319 ID=MS.gene56438
chr4.3 mRNA 27900319 27901423 27900319 ID=MS.gene56438.t1;Parent=MS.gene56438
chr4.3 exon 27900319 27900377 27900319 ID=MS.gene56438.t1.exon1;Parent=MS.gene56438.t1
chr4.3 CDS 27900319 27900377 27900319 ID=cds.MS.gene56438.t1;Parent=MS.gene56438.t1
chr4.3 exon 27900469 27900787 27900469 ID=MS.gene56438.t1.exon2;Parent=MS.gene56438.t1
chr4.3 CDS 27900469 27900787 27900469 ID=cds.MS.gene56438.t1;Parent=MS.gene56438.t1
chr4.3 exon 27900908 27901423 27900908 ID=MS.gene56438.t1.exon3;Parent=MS.gene56438.t1
chr4.3 CDS 27900908 27901423 27900908 ID=cds.MS.gene56438.t1;Parent=MS.gene56438.t1
Gene Sequence

>MS.gene56438

ATGGCAGTGGAACCTCCTCATATGAATTTCTTCTCTCCACAGTCACAAATCAATCACAGGTAATATATAACACATTCATTCTTTTCCTTACCTTCAGTTTTCTTATATTATACTATATAGTTTTAACACTTTTCCTCTGTTTTTCCACAGAGATATAACCAAATTCAATCAACAATTCTACAATGCTTCCATGAATGGTGCTATTCACATGCCTATGTCTTCAACAATCCCTGAATCGTTCTTTCCTTTTCATCAACCATCTTTCTGTGAACCAAAAGCTGATAGTACTGTCACCTATCACATCCCTGATTCAAGAAAGCGTTTCAGAGACTCCACTGAAGAATCATACACTCTTCCTCTTCCTCAGAAAAATATCAAACTCTCATCTCAACCTTCCTTTGTTGATCAGAATCTCCTCTATCATCTCCAAAATCAACAATCTGAAATCGATCTCTTCATTGCTCAACATGTAAGTCATTCATTCAAGAAAAAAGAAACTGTTAAAAGGAAGTTACAGATTTATTGCTTAGATTTTGTGATTATGGTTAATTATTAGTAATTAATATTGTTAATTTTTGTTGATTAACAGACAGAGAGAGTGAGAATGGAGATAGAAGAGCAAAGCATGAAGCAATCAAGAATGCTACAAGCGGCAATCCAAGAAGCAGTAACAAAGAAACTGAAGCAAAAAGAAGAAGAGATTCAGAGAATGGAGAAACAAAATCTAATGCTACAAGAAAAAGCGAAAACACTGATTATGGAGAATCAGATTTGGAGAGAAATGGCTTTGACAAACGAATCTGCTGTAAACACGTTACGTAACGAATTAGAACAAGTTCTGGCACATGTTGAGAATCACCGTAACGATGAAGATGCAGCGTCGAGTTGTGGAAGTAACCACCACGTGAAGGAGGAGGTGGTGGTGGAAGAAGCGTCGTCGGCGGTGGTTGGGAAATTGTGCAGCGGTTGTGGGGTAAGGGAGTCAGTAGTGCTGTTATTGCCATGTAGGCATTTGTGTTTGTGTACAATGTGTGGGACCCACATTCGTAATTGCCCTCTTTGTTTTTCTGGAATTAATGCTAGTGTTCATGTAAATTCTTCTTAG

Protein sequence

>MS.gene56438.t1

MAVEPPHMNFFSPQSQINHRDITKFNQQFYNASMNGAIHMPMSSTIPESFFPFHQPSFCEPKADSTVTYHIPDSRKRFRDSTEESYTLPLPQKNIKLSSQPSFVDQNLLYHLQNQQSEIDLFIAQHTERVRMEIEEQSMKQSRMLQAAIQEAVTKKLKQKEEEIQRMEKQNLMLQEKAKTLIMENQIWREMALTNESAVNTLRNELEQVLAHVENHRNDEDAASSCGSNHHVKEEVVVEEASSAVVGKLCSGCGVRESVVLLLPCRHLCLCTMCGTHIRNCPLCFSGINASVHVNSS