Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74604.t1 | XP_003594454.1 | 86.4 | 280 | 36 | 1 | 3 | 280 | 40 | 319 | 3.70E-126 | 461.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74604.t1 | O81851 | 46.0 | 250 | 123 | 4 | 37 | 280 | 60 | 303 | 6.0e-48 | 192.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74604.t1 | G7IFV4 | 86.4 | 280 | 36 | 1 | 3 | 280 | 40 | 319 | 2.7e-126 | 461.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene74604 | 0.818123 | 2.31E-52 | -1.69E-46 |
| MS.gene04918 | MS.gene74604 | 0.855597 | 5.67E-62 | -1.69E-46 |
| MS.gene04919 | MS.gene74604 | 0.824714 | 6.95E-54 | -1.69E-46 |
| MS.gene04920 | MS.gene74604 | 0.851167 | 1.06E-60 | -1.69E-46 |
| MS.gene049254 | MS.gene74604 | 0.867832 | 1.02E-65 | -1.69E-46 |
| MS.gene049283 | MS.gene74604 | 0.862671 | 4.30E-64 | -1.69E-46 |
| MS.gene049343 | MS.gene74604 | 0.81534 | 9.72E-52 | -1.69E-46 |
| MS.gene049350 | MS.gene74604 | 0.803205 | 3.90E-49 | -1.69E-46 |
| MS.gene049368 | MS.gene74604 | 0.806621 | 7.53E-50 | -1.69E-46 |
| MS.gene049419 | MS.gene74604 | -0.831556 | 1.56E-55 | -1.69E-46 |
| MS.gene049499 | MS.gene74604 | 0.856043 | 4.20E-62 | -1.69E-46 |
| MS.gene049652 | MS.gene74604 | 0.836723 | 7.92E-57 | -1.69E-46 |
| MS.gene049680 | MS.gene74604 | 0.832952 | 7.05E-56 | -1.69E-46 |
| MS.gene049711 | MS.gene74604 | 0.815111 | 1.09E-51 | -1.69E-46 |
| MS.gene049807 | MS.gene74604 | 0.81082 | 9.53E-51 | -1.69E-46 |
| MS.gene049983 | MS.gene74604 | 0.831274 | 1.83E-55 | -1.69E-46 |
| MS.gene050559 | MS.gene74604 | 0.852436 | 4.62E-61 | -1.69E-46 |
| MS.gene050561 | MS.gene74604 | 0.842913 | 1.93E-58 | -1.69E-46 |
| MS.gene050562 | MS.gene74604 | 0.837486 | 5.05E-57 | -1.69E-46 |
| MS.gene050646 | MS.gene74604 | 0.802491 | 5.47E-49 | -1.69E-46 |
| MS.gene050945 | MS.gene74604 | 0.836651 | 8.26E-57 | -1.69E-46 |
| MS.gene050955 | MS.gene74604 | 0.816242 | 6.12E-52 | -1.69E-46 |
| MS.gene051003 | MS.gene74604 | 0.835087 | 2.06E-56 | -1.69E-46 |
| MS.gene051019 | MS.gene74604 | 0.802626 | 5.13E-49 | -1.69E-46 |
| MS.gene051234 | MS.gene74604 | 0.811196 | 7.90E-51 | -1.69E-46 |
| MS.gene051332 | MS.gene74604 | 0.812839 | 3.46E-51 | -1.69E-46 |
| MS.gene051343 | MS.gene74604 | 0.832926 | 7.15E-56 | -1.69E-46 |
| MS.gene051363 | MS.gene74604 | 0.814233 | 1.71E-51 | -1.69E-46 |
| MS.gene051597 | MS.gene74604 | 0.832246 | 1.05E-55 | -1.69E-46 |
| MS.gene051699 | MS.gene74604 | 0.826064 | 3.33E-54 | -1.69E-46 |
| MS.gene051744 | MS.gene74604 | 0.856843 | 2.45E-62 | -1.69E-46 |
| MS.gene051832 | MS.gene74604 | 0.813888 | 2.04E-51 | -1.69E-46 |
| MS.gene051848 | MS.gene74604 | -0.801907 | 7.21E-49 | -1.69E-46 |
| MS.gene051975 | MS.gene74604 | 0.85456 | 1.13E-61 | -1.69E-46 |
| MS.gene052072 | MS.gene74604 | 0.821735 | 3.45E-53 | -1.69E-46 |
| MS.gene052108 | MS.gene74604 | 0.824503 | 7.79E-54 | -1.69E-46 |
| MS.gene052307 | MS.gene74604 | 0.820756 | 5.80E-53 | -1.69E-46 |
| MS.gene052463 | MS.gene74604 | 0.806392 | 8.41E-50 | -1.69E-46 |
| MS.gene052500 | MS.gene74604 | 0.814993 | 1.16E-51 | -1.69E-46 |
| MS.gene052502 | MS.gene74604 | 0.802323 | 5.93E-49 | -1.69E-46 |
| MS.gene052518 | MS.gene74604 | 0.813829 | 2.10E-51 | -1.69E-46 |
| MS.gene052519 | MS.gene74604 | 0.817436 | 3.30E-52 | -1.69E-46 |
| MS.gene052533 | MS.gene74604 | 0.860726 | 1.69E-63 | -1.69E-46 |
| MS.gene052534 | MS.gene74604 | 0.836472 | 9.17E-57 | -1.69E-46 |
| MS.gene052582 | MS.gene74604 | 0.817518 | 3.16E-52 | -1.69E-46 |
| MS.gene052584 | MS.gene74604 | 0.824381 | 8.32E-54 | -1.69E-46 |
| MS.gene052685 | MS.gene74604 | 0.81113 | 8.17E-51 | -1.69E-46 |
| MS.gene052826 | MS.gene74604 | 0.808468 | 3.05E-50 | -1.69E-46 |
| MS.gene052889 | MS.gene74604 | 0.81474 | 1.32E-51 | -1.69E-46 |
| MS.gene052954 | MS.gene74604 | 0.827574 | 1.45E-54 | -1.69E-46 |
| MS.gene053006 | MS.gene74604 | 0.855347 | 6.70E-62 | -1.69E-46 |
| MS.gene053226 | MS.gene74604 | 0.841025 | 6.10E-58 | -1.69E-46 |
| MS.gene053228 | MS.gene74604 | 0.804308 | 2.30E-49 | -1.69E-46 |
| MS.gene053272 | MS.gene74604 | -0.837119 | 6.27E-57 | -1.69E-46 |
| MS.gene053298 | MS.gene74604 | 0.801675 | 8.05E-49 | -1.69E-46 |
| MS.gene05364 | MS.gene74604 | 0.826748 | 2.29E-54 | -1.69E-46 |
| MS.gene053931 | MS.gene74604 | 0.833446 | 5.31E-56 | -1.69E-46 |
| MS.gene053963 | MS.gene74604 | 0.837942 | 3.86E-57 | -1.69E-46 |
| MS.gene054004 | MS.gene74604 | 0.833686 | 4.62E-56 | -1.69E-46 |
| MS.gene054142 | MS.gene74604 | 0.816015 | 6.88E-52 | -1.69E-46 |
| MS.gene054222 | MS.gene74604 | 0.829029 | 6.47E-55 | -1.69E-46 |
| MS.gene054403 | MS.gene74604 | 0.803714 | 3.06E-49 | -1.69E-46 |
| MS.gene054479 | MS.gene74604 | 0.80717 | 5.76E-50 | -1.69E-46 |
| MS.gene054651 | MS.gene74604 | 0.847434 | 1.16E-59 | -1.69E-46 |
| MS.gene05469 | MS.gene74604 | 0.803426 | 3.51E-49 | -1.69E-46 |
| MS.gene054892 | MS.gene74604 | 0.828208 | 1.02E-54 | -1.69E-46 |
| MS.gene054893 | MS.gene74604 | 0.834455 | 2.97E-56 | -1.69E-46 |
| MS.gene054957 | MS.gene74604 | 0.846512 | 2.07E-59 | -1.69E-46 |
| MS.gene055150 | MS.gene74604 | 0.851561 | 8.19E-61 | -1.69E-46 |
| MS.gene055513 | MS.gene74604 | 0.83484 | 2.38E-56 | -1.69E-46 |
| MS.gene055555 | MS.gene74604 | 0.821387 | 4.15E-53 | -1.69E-46 |
| MS.gene055586 | MS.gene74604 | 0.832817 | 7.61E-56 | -1.69E-46 |
| MS.gene055589 | MS.gene74604 | 0.806382 | 8.45E-50 | -1.69E-46 |
| MS.gene055724 | MS.gene74604 | 0.812049 | 5.15E-51 | -1.69E-46 |
| MS.gene055801 | MS.gene74604 | 0.813664 | 2.28E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene74604 | 0.805644 | 1.21E-49 | -1.69E-46 |
| MS.gene055841 | MS.gene74604 | 0.852678 | 3.94E-61 | -1.69E-46 |
| MS.gene056699 | MS.gene74604 | 0.88505 | 1.14E-71 | -1.69E-46 |
| MS.gene056776 | MS.gene74604 | 0.824259 | 8.89E-54 | -1.69E-46 |
| MS.gene05686 | MS.gene74604 | 0.85146 | 8.75E-61 | -1.69E-46 |
| MS.gene05689 | MS.gene74604 | 0.806448 | 8.19E-50 | -1.69E-46 |
| MS.gene05690 | MS.gene74604 | 0.820319 | 7.30E-53 | -1.69E-46 |
| MS.gene056986 | MS.gene74604 | 0.849272 | 3.60E-60 | -1.69E-46 |
| MS.gene057034 | MS.gene74604 | 0.842352 | 2.73E-58 | -1.69E-46 |
| MS.gene057102 | MS.gene74604 | 0.904914 | 7.45E-80 | -1.69E-46 |
| MS.gene057155 | MS.gene74604 | 0.819773 | 9.74E-53 | -1.69E-46 |
| MS.gene057208 | MS.gene74604 | 0.885149 | 1.04E-71 | -1.69E-46 |
| MS.gene057371 | MS.gene74604 | 0.816441 | 5.52E-52 | -1.69E-46 |
| MS.gene057611 | MS.gene74604 | 0.815048 | 1.13E-51 | -1.69E-46 |
| MS.gene057705 | MS.gene74604 | 0.819657 | 1.04E-52 | -1.69E-46 |
| MS.gene058008 | MS.gene74604 | 0.853003 | 3.18E-61 | -1.69E-46 |
| MS.gene058009 | MS.gene74604 | 0.827952 | 1.18E-54 | -1.69E-46 |
| MS.gene058095 | MS.gene74604 | 0.821752 | 3.42E-53 | -1.69E-46 |
| MS.gene058159 | MS.gene74604 | 0.826617 | 2.46E-54 | -1.69E-46 |
| MS.gene058279 | MS.gene74604 | 0.811228 | 7.78E-51 | -1.69E-46 |
| MS.gene058592 | MS.gene74604 | 0.824414 | 8.18E-54 | -1.69E-46 |
| MS.gene058781 | MS.gene74604 | 0.86384 | 1.87E-64 | -1.69E-46 |
| MS.gene058783 | MS.gene74604 | 0.866692 | 2.37E-65 | -1.69E-46 |
| MS.gene05878 | MS.gene74604 | 0.822351 | 2.48E-53 | -1.69E-46 |
| MS.gene058979 | MS.gene74604 | 0.809354 | 1.97E-50 | -1.69E-46 |
| MS.gene059145 | MS.gene74604 | 0.828129 | 1.07E-54 | -1.69E-46 |
| MS.gene059146 | MS.gene74604 | 0.844649 | 6.63E-59 | -1.69E-46 |
| MS.gene059170 | MS.gene74604 | 0.801683 | 8.02E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene74604 | 0.869176 | 3.77E-66 | -1.69E-46 |
| MS.gene05985 | MS.gene74604 | -0.91435 | 2.13E-84 | -1.69E-46 |
| MS.gene059901 | MS.gene74604 | -0.859079 | 5.30E-63 | -1.69E-46 |
| MS.gene059935 | MS.gene74604 | 0.841633 | 4.22E-58 | -1.69E-46 |
| MS.gene059965 | MS.gene74604 | 0.814335 | 1.62E-51 | -1.69E-46 |
| MS.gene060244 | MS.gene74604 | 0.8401 | 1.07E-57 | -1.69E-46 |
| MS.gene060338 | MS.gene74604 | 0.815093 | 1.10E-51 | -1.69E-46 |
| MS.gene060365 | MS.gene74604 | 0.863632 | 2.17E-64 | -1.69E-46 |
| MS.gene060521 | MS.gene74604 | 0.802004 | 6.89E-49 | -1.69E-46 |
| MS.gene060527 | MS.gene74604 | 0.805968 | 1.03E-49 | -1.69E-46 |
| MS.gene060716 | MS.gene74604 | 0.819023 | 1.44E-52 | -1.69E-46 |
| MS.gene060763 | MS.gene74604 | 0.806603 | 7.59E-50 | -1.69E-46 |
| MS.gene060840 | MS.gene74604 | 0.818934 | 1.51E-52 | -1.69E-46 |
| MS.gene060932 | MS.gene74604 | 0.802729 | 4.89E-49 | -1.69E-46 |
| MS.gene060933 | MS.gene74604 | 0.83775 | 4.32E-57 | -1.69E-46 |
| MS.gene060935 | MS.gene74604 | 0.888664 | 4.83E-73 | -1.69E-46 |
| MS.gene060937 | MS.gene74604 | 0.887618 | 1.22E-72 | -1.69E-46 |
| MS.gene061100 | MS.gene74604 | 0.821232 | 4.51E-53 | -1.69E-46 |
| MS.gene061135 | MS.gene74604 | 0.806127 | 9.57E-50 | -1.69E-46 |
| MS.gene061188 | MS.gene74604 | 0.870812 | 1.10E-66 | -1.69E-46 |
| MS.gene061243 | MS.gene74604 | 0.806751 | 7.07E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene02027 | MS.gene74604 | PPI |
| MS.gene74604 | MS.gene067574 | PPI |
| MS.gene003226 | MS.gene74604 | PPI |
| MS.gene74604 | MS.gene02785 | PPI |
| MS.gene74604 | MS.gene56438 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74604.t1 | MTR_2g028830 | 97.872 | 282 | 4 | 1 | 1 | 280 | 38 | 319 | 0.0 | 576 |
| MS.gene74604.t1 | MTR_4g083540 | 52.837 | 282 | 117 | 8 | 2 | 280 | 25 | 293 | 3.76e-94 | 280 |
| MS.gene74604.t1 | MTR_2g069350 | 42.462 | 325 | 130 | 13 | 13 | 280 | 42 | 366 | 4.80e-68 | 215 |
| MS.gene74604.t1 | MTR_5g013690 | 31.863 | 204 | 117 | 4 | 88 | 280 | 80 | 272 | 1.26e-27 | 107 |
| MS.gene74604.t1 | MTR_5g013690 | 31.863 | 204 | 117 | 4 | 88 | 280 | 143 | 335 | 1.42e-27 | 109 |
| MS.gene74604.t1 | MTR_3g117970 | 35.955 | 178 | 88 | 5 | 91 | 267 | 31 | 183 | 4.10e-23 | 94.0 |
| MS.gene74604.t1 | MTR_1g019250 | 30.481 | 187 | 129 | 1 | 83 | 269 | 145 | 330 | 6.01e-23 | 96.7 |
| MS.gene74604.t1 | MTR_4g036485 | 27.358 | 212 | 130 | 3 | 66 | 267 | 2 | 199 | 6.66e-22 | 91.3 |
| MS.gene74604.t1 | MTR_4g099060 | 31.604 | 212 | 131 | 4 | 75 | 280 | 83 | 286 | 5.68e-21 | 90.5 |
| MS.gene74604.t1 | MTR_4g099060 | 31.604 | 212 | 131 | 4 | 75 | 280 | 128 | 331 | 8.26e-21 | 90.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74604.t1 | AT4G19700 | 50.735 | 272 | 120 | 6 | 15 | 280 | 40 | 303 | 1.15e-76 | 236 |
| MS.gene74604.t1 | AT5G45100 | 47.183 | 284 | 123 | 7 | 6 | 280 | 1 | 266 | 6.05e-75 | 230 |
| MS.gene74604.t1 | AT5G45100 | 46.853 | 286 | 125 | 7 | 4 | 280 | 26 | 293 | 1.63e-74 | 229 |
| MS.gene74604.t1 | AT3G12920 | 40.468 | 299 | 143 | 9 | 2 | 280 | 51 | 334 | 5.40e-62 | 199 |
| MS.gene74604.t1 | AT1G79110 | 48.624 | 218 | 95 | 3 | 80 | 280 | 115 | 332 | 1.98e-54 | 179 |
| MS.gene74604.t1 | AT1G79110 | 48.624 | 218 | 95 | 3 | 80 | 280 | 137 | 354 | 2.55e-54 | 180 |
| MS.gene74604.t1 | AT1G79110 | 47.964 | 221 | 95 | 4 | 80 | 280 | 137 | 357 | 2.94e-52 | 174 |
| MS.gene74604.t1 | AT1G45976 | 32.673 | 202 | 125 | 4 | 71 | 269 | 122 | 315 | 9.00e-29 | 112 |
| MS.gene74604.t1 | AT1G60610 | 30.841 | 214 | 140 | 2 | 70 | 277 | 126 | 337 | 9.23e-26 | 104 |
| MS.gene74604.t1 | AT1G60610 | 30.841 | 214 | 140 | 2 | 70 | 277 | 126 | 337 | 9.23e-26 | 104 |
| MS.gene74604.t1 | AT1G60610 | 30.841 | 214 | 140 | 2 | 70 | 277 | 126 | 337 | 9.23e-26 | 104 |
| MS.gene74604.t1 | AT1G60610 | 30.841 | 214 | 140 | 2 | 70 | 277 | 126 | 337 | 9.23e-26 | 104 |
| MS.gene74604.t1 | AT1G60610 | 30.841 | 214 | 140 | 2 | 70 | 277 | 126 | 337 | 9.23e-26 | 104 |
| MS.gene74604.t1 | AT1G10650 | 32.075 | 212 | 124 | 4 | 80 | 280 | 81 | 283 | 3.24e-24 | 99.4 |
| MS.gene74604.t1 | AT1G10650 | 32.075 | 212 | 124 | 4 | 80 | 280 | 137 | 339 | 7.46e-24 | 99.4 |
| MS.gene74604.t1 | AT4G35070 | 30.601 | 183 | 121 | 3 | 88 | 269 | 22 | 199 | 9.70e-22 | 90.9 |
| MS.gene74604.t1 | AT4G35070 | 30.601 | 183 | 121 | 3 | 88 | 269 | 77 | 254 | 1.27e-21 | 91.7 |
| MS.gene74604.t1 | AT1G32740 | 31.111 | 225 | 136 | 7 | 65 | 275 | 44 | 263 | 2.41e-20 | 88.2 |
| MS.gene74604.t1 | AT1G32740 | 31.111 | 225 | 136 | 7 | 65 | 275 | 44 | 263 | 2.41e-20 | 88.2 |
| MS.gene74604.t1 | AT1G32740 | 31.111 | 225 | 136 | 7 | 65 | 275 | 88 | 307 | 3.78e-20 | 88.6 |
| MS.gene74604.t1 | AT1G32740 | 31.111 | 225 | 136 | 7 | 65 | 275 | 77 | 296 | 3.83e-20 | 88.6 |
| MS.gene74604.t1 | AT5G47050 | 26.400 | 250 | 156 | 4 | 35 | 280 | 75 | 300 | 2.44e-19 | 86.3 |
| MS.gene74604.t1 | AT4G17680 | 25.385 | 260 | 153 | 7 | 51 | 277 | 61 | 312 | 2.66e-18 | 83.6 |
| MS.gene74604.t1 | AT4G17680 | 25.628 | 199 | 134 | 4 | 85 | 277 | 50 | 240 | 1.65e-17 | 80.1 |
| MS.gene74604.t1 | AT4G17680 | 26.087 | 184 | 122 | 4 | 100 | 277 | 13 | 188 | 6.36e-14 | 69.3 |
| MS.gene74604.t1 | AT2G12290 | 50.769 | 65 | 31 | 1 | 143 | 207 | 28 | 91 | 4.76e-12 | 62.4 |
Find 55 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAATTGTTACGTAAATAAT+TGG | 0.209804 | 2.3:+60586656 | None:intergenic |
| CAAACATTGGATTGATAAAA+TGG | 0.230510 | 2.3:+60587732 | None:intergenic |
| AAAGAATAGGGAAGCTAAAT+TGG | 0.250485 | 2.3:-60586758 | MS.gene74604:CDS |
| CAATTATTTACGTAACAATT+TGG | 0.251798 | 2.3:-60586655 | MS.gene74604:CDS |
| TTGTGTTGTTACTACCTTGT+AGG | 0.317083 | 2.3:-60586458 | MS.gene74604:CDS |
| GATGAAGAGATTCAAAGAAT+AGG | 0.332254 | 2.3:-60586771 | MS.gene74604:CDS |
| ATGAAGAGATTCAAAGAATA+GGG | 0.348961 | 2.3:-60586770 | MS.gene74604:CDS |
| AAGTTTAAGTGCTGAAAATC+AGG | 0.355207 | 2.3:-60586715 | MS.gene74604:CDS |
| GAAAGTGAGAATGGAGTTAG+AGG | 0.361074 | 2.3:-60586868 | MS.gene74604:CDS |
| ATAGATTCATGCATTGTTGT+TGG | 0.375132 | 2.3:+60587759 | None:intergenic |
| TAACAATTTGGAACAAGTTA+TGG | 0.392210 | 2.3:-60586643 | MS.gene74604:CDS |
| GACTGTCGGTGGTGGTGGTT+TGG | 0.395247 | 2.3:-60586517 | MS.gene74604:CDS |
| TGCGATCAATATCAGATTGC+TGG | 0.414409 | 2.3:+60587538 | None:intergenic |
| ATGACGCTGAATCGAGTTGC+GGG | 0.417866 | 2.3:-60586578 | MS.gene74604:CDS |
| TCGGTGGTGGTGGTTTGGTT+AGG | 0.423324 | 2.3:-60586512 | MS.gene74604:CDS |
| AGACCACTATCAGCTCTGTT+TGG | 0.425440 | 2.3:+60587705 | None:intergenic |
| GTTAGGATGTGTAAGAATTG+TGG | 0.438375 | 2.3:-60586495 | MS.gene74604:CDS |
| TCATCATGCCGCTGCGGCCG+AGG | 0.450535 | 2.3:-60586601 | MS.gene74604:CDS |
| TCATGCATTGTTGTTGGAAG+AGG | 0.451659 | 2.3:+60587765 | None:intergenic |
| ACAGAATGAGAAAGTGAGAA+TGG | 0.452277 | 2.3:-60586877 | MS.gene74604:intron |
| TAAGTGCTGAAAATCAGGTT+TGG | 0.460640 | 2.3:-60586710 | MS.gene74604:CDS |
| CAAAGGCACAAATGCCTACA+AGG | 0.486790 | 2.3:+60586444 | None:intergenic |
| TGTGCCTTTGCAATGTGTGC+GGG | 0.488765 | 2.3:-60586431 | MS.gene74604:CDS |
| GCGGCGACTGTCGGTGGTGG+TGG | 0.490968 | 2.3:-60586522 | MS.gene74604:CDS |
| TTAGGATGTGTAAGAATTGT+GGG | 0.497897 | 2.3:-60586494 | MS.gene74604:CDS |
| TGCTGAAAATCAGGTTTGGA+GGG | 0.519581 | 2.3:-60586706 | MS.gene74604:CDS |
| GTGTAAGAATTGTGGGGTGA+GGG | 0.520811 | 2.3:-60586487 | MS.gene74604:CDS |
| GTGCTGAAAATCAGGTTTGG+AGG | 0.526743 | 2.3:-60586707 | MS.gene74604:CDS |
| ACTCGATTCAGCGTCATCCT+CGG | 0.538662 | 2.3:+60586584 | None:intergenic |
| TGTGTAAGAATTGTGGGGTG+AGG | 0.539846 | 2.3:-60586488 | MS.gene74604:CDS |
| GAATCGAGTTGCGGGAGTAA+TGG | 0.541126 | 2.3:-60586570 | MS.gene74604:CDS |
| AGCGTCATCCTCGGCCGCAG+CGG | 0.547182 | 2.3:+60586593 | None:intergenic |
| AAATGTCCAGTGTGTGACTC+TGG | 0.547650 | 2.3:-60586396 | MS.gene74604:CDS |
| ACCGCGGCGACTGTCGGTGG+TGG | 0.548750 | 2.3:-60586525 | MS.gene74604:CDS |
| GAGTTGCGGGAGTAATGGTC+CGG | 0.554586 | 2.3:-60586565 | MS.gene74604:CDS |
| TCTGTTTGGATCACAAACAT+TGG | 0.555502 | 2.3:+60587719 | None:intergenic |
| TGAATTATACAATGTTCAGA+TGG | 0.557789 | 2.3:-60587799 | MS.gene74604:CDS |
| GTGTCTTCACCATCGTCAGC+CGG | 0.559591 | 2.3:+60586546 | None:intergenic |
| AATGAGGCAATCAAGGATGT+TGG | 0.559955 | 2.3:-60586838 | MS.gene74604:CDS |
| GAAGACACCGCGGCGACTGT+CGG | 0.571935 | 2.3:-60586531 | MS.gene74604:CDS |
| TTGTGCCTTTGCAATGTGTG+CGG | 0.572306 | 2.3:-60586432 | MS.gene74604:CDS |
| TCCAGTGTGTGACTCTGGCA+TGG | 0.597448 | 2.3:-60586391 | MS.gene74604:CDS |
| CACTGGACATTTACGAACAG+TGG | 0.609918 | 2.3:+60586407 | None:intergenic |
| GACACCGCGGCGACTGTCGG+TGG | 0.614188 | 2.3:-60586528 | MS.gene74604:CDS |
| AGTAATGGTCCGGCTGACGA+TGG | 0.620198 | 2.3:-60586555 | MS.gene74604:CDS |
| AGTTATGGCACATGTCAATG+AGG | 0.626941 | 2.3:-60586628 | MS.gene74604:CDS |
| GATCCAAACAGAGCTGATAG+TGG | 0.638226 | 2.3:-60587708 | MS.gene74604:CDS |
| GATGACGCTGAATCGAGTTG+CGG | 0.639252 | 2.3:-60586579 | MS.gene74604:CDS |
| GTTATGGCACATGTCAATGA+GGG | 0.650135 | 2.3:-60586627 | MS.gene74604:CDS |
| TCCATGCCAGAGTCACACAC+TGG | 0.661955 | 2.3:+60586390 | None:intergenic |
| TGGACCCGCACACATTGCAA+AGG | 0.662474 | 2.3:+60586427 | None:intergenic |
| GGGACATCATCATGCCGCTG+CGG | 0.686145 | 2.3:-60586607 | MS.gene74604:CDS |
| ACCACCACCGACAGTCGCCG+CGG | 0.694431 | 2.3:+60586524 | None:intergenic |
| TAGGATGTGTAAGAATTGTG+GGG | 0.749557 | 2.3:-60586493 | MS.gene74604:CDS |
| TGACGATGGTGAAGACACCG+CGG | 0.808171 | 2.3:-60586541 | MS.gene74604:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATAAATAAATTATATT+TGG | - | chr2.3:60587034-60587053 | MS.gene74604:intron | 0.0% |
| !! | AAAATAAATAAATTATATTT+GGG | - | chr2.3:60587035-60587054 | MS.gene74604:intron | 0.0% |
| !! | TTTAAAAATAAATAAAAAAA+CGG | + | chr2.3:60586905-60586924 | None:intergenic | 0.0% |
| !!! | TTTTATTTATTTTTAAATTA+TGG | - | chr2.3:60586908-60586927 | MS.gene74604:intron | 0.0% |
| !!! | TATTTTTATGATAATCAAAA+CGG | + | chr2.3:60587021-60587040 | None:intergenic | 10.0% |
| !!! | TATTTTTGCTAATATTTTTA+TGG | - | chr2.3:60587180-60587199 | MS.gene74604:intron | 10.0% |
| !! | ACTTTGAATGAAAATATTAA+TGG | - | chr2.3:60586848-60586867 | MS.gene74604:CDS | 15.0% |
| !! | ATAAACTAATAGTCTATAAA+AGG | - | chr2.3:60587068-60587087 | MS.gene74604:intron | 15.0% |
| !! | CTTTGAATGAAAATATTAAT+GGG | - | chr2.3:60586849-60586868 | MS.gene74604:CDS | 15.0% |
| !! | TTTGAATGAAAATATTAATG+GGG | - | chr2.3:60586850-60586869 | MS.gene74604:CDS | 15.0% |
| !!! | TTATTTTTAAATTATGGAGT+GGG | - | chr2.3:60586914-60586933 | MS.gene74604:intron | 15.0% |
| !!! | TTTAAGTTTTTTTTAACATG+CGG | - | chr2.3:60586966-60586985 | MS.gene74604:intron | 15.0% |
| !!! | TTTATTTTTAAATTATGGAG+TGG | - | chr2.3:60586913-60586932 | MS.gene74604:intron | 15.0% |
| !! | CAAATTGTTACGTAAATAAT+TGG | + | chr2.3:60587519-60587538 | None:intergenic | 20.0% |
| !! | CAATTATTTACGTAACAATT+TGG | - | chr2.3:60587517-60587536 | MS.gene74604:intron | 20.0% |
| !! | TAAATTGAGTAACTGATAAA+GGG | - | chr2.3:60586809-60586828 | MS.gene74604:CDS | 20.0% |
| !! | TAATTACACATAAATAGTAC+AGG | + | chr2.3:60587250-60587269 | None:intergenic | 20.0% |
| !! | TTGTGTTTGAAAAAGAAAAA+AGG | - | chr2.3:60586748-60586767 | MS.gene74604:CDS | 20.0% |
| !!! | GGTGTTTTATATTTTCTAAA+AGG | - | chr2.3:60586871-60586890 | MS.gene74604:CDS | 20.0% |
| !!! | TATTTTTAAATTATGGAGTG+GGG | - | chr2.3:60586915-60586934 | MS.gene74604:intron | 20.0% |
| ! | AAAAAATTACATGATGAGCT+AGG | + | chr2.3:60586661-60586680 | None:intergenic | 25.0% |
| ! | AGAGATAATTAAACAAGTGA+AGG | + | chr2.3:60586687-60586706 | None:intergenic | 25.0% |
| ! | ATGAAGAGATTCAAAGAATA+GGG | - | chr2.3:60587402-60587421 | MS.gene74604:intron | 25.0% |
| ! | CAAACATTGGATTGATAAAA+TGG | + | chr2.3:60586443-60586462 | None:intergenic | 25.0% |
| ! | CTAAATTGAGTAACTGATAA+AGG | - | chr2.3:60586808-60586827 | MS.gene74604:CDS | 25.0% |
| ! | TGAATTATACAATGTTCAGA+TGG | - | chr2.3:60586373-60586392 | MS.gene74604:CDS | 25.0% |
| !! | ATTACTCTATTCTTTTGATG+AGG | + | chr2.3:60586560-60586579 | None:intergenic | 25.0% |
| !! | TAACAATTTGGAACAAGTTA+TGG | - | chr2.3:60587529-60587548 | MS.gene74604:CDS | 25.0% |
| !!! | AAGAGATTTTTCAACTGAAT+TGG | - | chr2.3:60586523-60586542 | MS.gene74604:CDS | 25.0% |
| AAAGAATAGGGAAGCTAAAT+TGG | - | chr2.3:60587414-60587433 | MS.gene74604:intron | 30.0% | |
| AACAAAAAATGAGGCAATCA+AGG | - | chr2.3:60587327-60587346 | MS.gene74604:intron | 30.0% | |
| AGTTAGAGGAACAAAAAATG+AGG | - | chr2.3:60587318-60587337 | MS.gene74604:intron | 30.0% | |
| ATAGATTCATGCATTGTTGT+TGG | + | chr2.3:60586416-60586435 | None:intergenic | 30.0% | |
| ATGCGGAGAAAACTTAAATA+GGG | - | chr2.3:60586983-60587002 | MS.gene74604:intron | 30.0% | |
| GATGAAGAGATTCAAAGAAT+AGG | - | chr2.3:60587401-60587420 | MS.gene74604:intron | 30.0% | |
| TTAGGATGTGTAAGAATTGT+GGG | - | chr2.3:60587678-60587697 | MS.gene74604:CDS | 30.0% | |
| !! | AAGTTTAAGTGCTGAAAATC+AGG | - | chr2.3:60587457-60587476 | MS.gene74604:intron | 30.0% |
| ACAGAATGAGAAAGTGAGAA+TGG | - | chr2.3:60587295-60587314 | MS.gene74604:intron | 35.0% | |
| CATGCGGAGAAAACTTAAAT+AGG | - | chr2.3:60586982-60587001 | MS.gene74604:intron | 35.0% | |
| GTTAGGATGTGTAAGAATTG+TGG | - | chr2.3:60587677-60587696 | MS.gene74604:CDS | 35.0% | |
| TAGGATGTGTAAGAATTGTG+GGG | - | chr2.3:60587679-60587698 | MS.gene74604:CDS | 35.0% | |
| TCTGTTTGGATCACAAACAT+TGG | + | chr2.3:60586456-60586475 | None:intergenic | 35.0% | |
| ! | ACTCTATTCTTTTGATGAGG+TGG | + | chr2.3:60586557-60586576 | None:intergenic | 35.0% |
| ! | TTGTGTTGTTACTACCTTGT+AGG | - | chr2.3:60587714-60587733 | MS.gene74604:CDS | 35.0% |
| !! | CTTGAACGTTTTCTTTGAAG+AGG | + | chr2.3:60586506-60586525 | None:intergenic | 35.0% |
| !! | TAAGTGCTGAAAATCAGGTT+TGG | - | chr2.3:60587462-60587481 | MS.gene74604:intron | 35.0% |
| AATGAGGCAATCAAGGATGT+TGG | - | chr2.3:60587334-60587353 | MS.gene74604:intron | 40.0% | |
| AGTTATGGCACATGTCAATG+AGG | - | chr2.3:60587544-60587563 | MS.gene74604:CDS | 40.0% | |
| GAAAGTGAGAATGGAGTTAG+AGG | - | chr2.3:60587304-60587323 | MS.gene74604:intron | 40.0% | |
| GTTATGGCACATGTCAATGA+GGG | - | chr2.3:60587545-60587564 | MS.gene74604:CDS | 40.0% | |
| TGCGATCAATATCAGATTGC+TGG | + | chr2.3:60586637-60586656 | None:intergenic | 40.0% | |
| TGCTGAAAATCAGGTTTGGA+GGG | - | chr2.3:60587466-60587485 | MS.gene74604:intron | 40.0% | |
| ! | TCATGCATTGTTGTTGGAAG+AGG | + | chr2.3:60586410-60586429 | None:intergenic | 40.0% |
| AAATGTCCAGTGTGTGACTC+TGG | - | chr2.3:60587776-60587795 | MS.gene74604:CDS | 45.0% | |
| AGACCACTATCAGCTCTGTT+TGG | + | chr2.3:60586470-60586489 | None:intergenic | 45.0% | |
| CACTGGACATTTACGAACAG+TGG | + | chr2.3:60587768-60587787 | None:intergenic | 45.0% | |
| GATCCAAACAGAGCTGATAG+TGG | - | chr2.3:60586464-60586483 | MS.gene74604:CDS | 45.0% | |
| GTGCTGAAAATCAGGTTTGG+AGG | - | chr2.3:60587465-60587484 | MS.gene74604:intron | 45.0% | |
| GTGTAAGAATTGTGGGGTGA+GGG | - | chr2.3:60587685-60587704 | MS.gene74604:CDS | 45.0% | |
| TGTGTAAGAATTGTGGGGTG+AGG | - | chr2.3:60587684-60587703 | MS.gene74604:CDS | 45.0% | |
| TTGTGCCTTTGCAATGTGTG+CGG | - | chr2.3:60587740-60587759 | MS.gene74604:CDS | 45.0% | |
| ! | CAAAGGCACAAATGCCTACA+AGG | + | chr2.3:60587731-60587750 | None:intergenic | 45.0% |
| !! | AAATAAATAAATTATATTTG+GGG | - | chr2.3:60587036-60587055 | MS.gene74604:intron | 5.0% |
| ACTCGATTCAGCGTCATCCT+CGG | + | chr2.3:60587591-60587610 | None:intergenic | 50.0% | |
| TGTGCCTTTGCAATGTGTGC+GGG | - | chr2.3:60587741-60587760 | MS.gene74604:CDS | 50.0% | |
| ! | ATGACGCTGAATCGAGTTGC+GGG | - | chr2.3:60587594-60587613 | MS.gene74604:CDS | 50.0% |
| ! | GAATCGAGTTGCGGGAGTAA+TGG | - | chr2.3:60587602-60587621 | MS.gene74604:CDS | 50.0% |
| ! | GATGACGCTGAATCGAGTTG+CGG | - | chr2.3:60587593-60587612 | MS.gene74604:CDS | 50.0% |
| AGTAATGGTCCGGCTGACGA+TGG | - | chr2.3:60587617-60587636 | MS.gene74604:CDS | 55.0% | |
| GAGTTGCGGGAGTAATGGTC+CGG | - | chr2.3:60587607-60587626 | MS.gene74604:CDS | 55.0% | |
| GTGTCTTCACCATCGTCAGC+CGG | + | chr2.3:60587629-60587648 | None:intergenic | 55.0% | |
| TCCAGTGTGTGACTCTGGCA+TGG | - | chr2.3:60587781-60587800 | MS.gene74604:CDS | 55.0% | |
| TCCATGCCAGAGTCACACAC+TGG | + | chr2.3:60587785-60587804 | None:intergenic | 55.0% | |
| TGACGATGGTGAAGACACCG+CGG | - | chr2.3:60587631-60587650 | MS.gene74604:CDS | 55.0% | |
| TGGACCCGCACACATTGCAA+AGG | + | chr2.3:60587748-60587767 | None:intergenic | 55.0% | |
| !! | TCGGTGGTGGTGGTTTGGTT+AGG | - | chr2.3:60587660-60587679 | MS.gene74604:CDS | 55.0% |
| GGGACATCATCATGCCGCTG+CGG | - | chr2.3:60587565-60587584 | MS.gene74604:CDS | 60.0% | |
| !! | GACTGTCGGTGGTGGTGGTT+TGG | - | chr2.3:60587655-60587674 | MS.gene74604:CDS | 60.0% |
| GAAGACACCGCGGCGACTGT+CGG | - | chr2.3:60587641-60587660 | MS.gene74604:CDS | 65.0% | |
| ACCACCACCGACAGTCGCCG+CGG | + | chr2.3:60587651-60587670 | None:intergenic | 70.0% | |
| AGCGTCATCCTCGGCCGCAG+CGG | + | chr2.3:60587582-60587601 | None:intergenic | 70.0% | |
| TCATCATGCCGCTGCGGCCG+AGG | - | chr2.3:60587571-60587590 | MS.gene74604:CDS | 70.0% | |
| ACCGCGGCGACTGTCGGTGG+TGG | - | chr2.3:60587647-60587666 | MS.gene74604:CDS | 75.0% | |
| GACACCGCGGCGACTGTCGG+TGG | - | chr2.3:60587644-60587663 | MS.gene74604:CDS | 75.0% | |
| ! | GCGGCGACTGTCGGTGGTGG+TGG | - | chr2.3:60587650-60587669 | MS.gene74604:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 60586362 | 60587832 | 60586362 | ID=MS.gene74604 |
| chr2.3 | mRNA | 60586362 | 60587832 | 60586362 | ID=MS.gene74604.t1;Parent=MS.gene74604 |
| chr2.3 | exon | 60587524 | 60587832 | 60587524 | ID=MS.gene74604.t1.exon1;Parent=MS.gene74604.t1 |
| chr2.3 | CDS | 60587524 | 60587832 | 60587524 | ID=cds.MS.gene74604.t1;Parent=MS.gene74604.t1 |
| chr2.3 | exon | 60586362 | 60586895 | 60586362 | ID=MS.gene74604.t1.exon2;Parent=MS.gene74604.t1 |
| chr2.3 | CDS | 60586362 | 60586895 | 60586362 | ID=cds.MS.gene74604.t1;Parent=MS.gene74604.t1 |
| Gene Sequence |
| Protein sequence |
