Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56528.t1 | G7JG80.1 | 96.8 | 189 | 6 | 0 | 1 | 189 | 1 | 189 | 1.70E-90 | 342 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56528.t1 | G7JG80 | 96.8 | 189 | 6 | 0 | 1 | 189 | 1 | 189 | 4.1e-93 | 342.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56528.t1 | A0A396IGM0 | 96.8 | 189 | 6 | 0 | 1 | 189 | 31 | 219 | 1.2e-90 | 342.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene56528 | 0.811732 | 6.04E-51 | -1.69E-46 |
| MS.gene052211 | MS.gene56528 | 0.809503 | 1.83E-50 | -1.69E-46 |
| MS.gene056920 | MS.gene56528 | 0.809967 | 1.46E-50 | -1.69E-46 |
| MS.gene056922 | MS.gene56528 | 0.804363 | 2.24E-49 | -1.69E-46 |
| MS.gene056923 | MS.gene56528 | 0.839138 | 1.90E-57 | -1.69E-46 |
| MS.gene056924 | MS.gene56528 | 0.81727 | 3.60E-52 | -1.69E-46 |
| MS.gene057261 | MS.gene56528 | 0.807434 | 5.07E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56528.t1 | MTR_4g081880 | 96.825 | 189 | 6 | 0 | 1 | 189 | 1 | 189 | 1.10e-131 | 367 |
| MS.gene56528.t1 | MTR_5g041900 | 65.775 | 187 | 63 | 1 | 1 | 187 | 1 | 186 | 2.61e-78 | 232 |
| MS.gene56528.t1 | MTR_7g011090 | 56.216 | 185 | 79 | 1 | 2 | 186 | 3 | 185 | 1.56e-69 | 209 |
| MS.gene56528.t1 | MTR_1g058560 | 41.885 | 191 | 105 | 1 | 2 | 186 | 4 | 194 | 6.17e-39 | 132 |
| MS.gene56528.t1 | MTR_2g086740 | 42.614 | 176 | 101 | 0 | 12 | 187 | 45 | 220 | 1.82e-37 | 129 |
| MS.gene56528.t1 | MTR_1g058570 | 42.857 | 189 | 103 | 1 | 3 | 186 | 5 | 193 | 8.78e-37 | 126 |
| MS.gene56528.t1 | MTR_1g058540 | 41.040 | 173 | 102 | 0 | 14 | 186 | 23 | 195 | 2.86e-36 | 125 |
| MS.gene56528.t1 | MTR_2g086740 | 44.340 | 106 | 59 | 0 | 12 | 117 | 45 | 150 | 1.54e-20 | 84.0 |
| MS.gene56528.t1 | MTR_0004s0210 | 35.220 | 159 | 94 | 2 | 22 | 180 | 1 | 150 | 5.37e-18 | 77.4 |
| MS.gene56528.t1 | MTR_7g108130 | 30.519 | 154 | 102 | 3 | 27 | 177 | 27 | 178 | 5.76e-18 | 77.8 |
| MS.gene56528.t1 | MTR_7g083120 | 32.099 | 162 | 105 | 3 | 10 | 169 | 11 | 169 | 3.13e-14 | 67.8 |
| MS.gene56528.t1 | MTR_4g118810 | 27.273 | 143 | 95 | 2 | 30 | 164 | 34 | 175 | 6.47e-14 | 67.4 |
| MS.gene56528.t1 | MTR_5g076480 | 33.043 | 115 | 75 | 1 | 66 | 180 | 68 | 180 | 8.10e-13 | 64.7 |
| MS.gene56528.t1 | MTR_8g089300 | 30.189 | 159 | 96 | 4 | 33 | 180 | 30 | 184 | 4.13e-12 | 62.4 |
| MS.gene56528.t1 | MTR_4g118800 | 31.515 | 165 | 97 | 8 | 27 | 184 | 26 | 181 | 1.33e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56528.t1 | AT5G15290 | 56.548 | 168 | 70 | 2 | 1 | 167 | 1 | 166 | 2.00e-62 | 191 |
| MS.gene56528.t1 | AT1G14160 | 47.368 | 190 | 95 | 1 | 2 | 186 | 19 | 208 | 8.88e-60 | 186 |
| MS.gene56528.t1 | AT2G27370 | 48.503 | 167 | 86 | 0 | 21 | 187 | 54 | 220 | 8.79e-48 | 155 |
| MS.gene56528.t1 | AT2G36100 | 44.767 | 172 | 95 | 0 | 15 | 186 | 34 | 205 | 7.32e-42 | 140 |
| MS.gene56528.t1 | AT5G06200 | 50.000 | 166 | 83 | 0 | 21 | 186 | 36 | 201 | 9.81e-41 | 137 |
| MS.gene56528.t1 | AT3G11550 | 45.614 | 171 | 93 | 0 | 16 | 186 | 33 | 203 | 2.64e-37 | 128 |
| MS.gene56528.t1 | AT4G15620 | 30.159 | 189 | 106 | 6 | 10 | 184 | 13 | 189 | 5.55e-18 | 78.2 |
| MS.gene56528.t1 | AT1G03700 | 32.895 | 152 | 87 | 3 | 32 | 180 | 12 | 151 | 1.75e-16 | 73.6 |
| MS.gene56528.t1 | AT4G15630 | 27.604 | 192 | 119 | 6 | 4 | 184 | 7 | 189 | 3.51e-16 | 73.2 |
| MS.gene56528.t1 | AT4G03540 | 38.095 | 105 | 52 | 2 | 68 | 169 | 46 | 140 | 6.26e-14 | 66.6 |
| MS.gene56528.t1 | AT3G06390 | 28.467 | 137 | 93 | 2 | 29 | 160 | 37 | 173 | 4.50e-11 | 59.7 |
Find 38 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTCTGCGGACGCATTTC+CGG | 0.160143 | 4.4:-30466738 | MS.gene56528:CDS |
| ACTTGGAAGTGCATTGAGTA+TGG | 0.314679 | 4.4:-30467603 | MS.gene56528:CDS |
| GTCTCCACTATTTCAATGCT+TGG | 0.317947 | 4.4:+30466638 | None:intergenic |
| ATGTTTGGTCTTCTTACATC+TGG | 0.318237 | 4.4:-30466846 | MS.gene56528:CDS |
| GAAGAGAGGGCTTTCAATAA+TGG | 0.375320 | 4.4:-30467660 | MS.gene56528:CDS |
| TGTCATTCTTCCACATTGTA+AGG | 0.389122 | 4.4:-30467354 | MS.gene56528:CDS |
| GGACTATTTCAGTGAAAAGT+AGG | 0.406947 | 4.4:-30467333 | MS.gene56528:CDS |
| CGCATTTCCGGTTCACTCGT+TGG | 0.423380 | 4.4:-30466726 | MS.gene56528:CDS |
| ATGATACTTATTCTCATGTC+AGG | 0.427186 | 4.4:-30466678 | MS.gene56528:CDS |
| GTAGCCACATACAATAGAAT+TGG | 0.428984 | 4.4:+30467396 | None:intergenic |
| ATTGTTTATGTAGCTCACTA+TGG | 0.441275 | 4.4:-30466804 | MS.gene56528:CDS |
| GGAGCATCATCCCAAAGAAA+AGG | 0.450818 | 4.4:-30467686 | MS.gene56528:CDS |
| TCTCTTGGTCTTTCTTGATA+CGG | 0.450983 | 4.4:-30467308 | MS.gene56528:intron |
| GAACTTGTGAAGGTTATGTT+TGG | 0.469308 | 4.4:-30466861 | MS.gene56528:intron |
| ATGGAAATCCCAGTGCCAAT+TGG | 0.476221 | 4.4:-30466785 | MS.gene56528:CDS |
| GAAAGAGCCAACGAGTGAAC+CGG | 0.477626 | 4.4:+30466719 | None:intergenic |
| CTAACAAAACGTGTGGCAAA+AGG | 0.478599 | 4.4:+30467560 | None:intergenic |
| AGTGAAAAGTAGGATTCTCT+TGG | 0.484716 | 4.4:-30467323 | MS.gene56528:CDS |
| GAAAGGAAACCAATTGGCAC+TGG | 0.490083 | 4.4:+30466776 | None:intergenic |
| CGCAGAAGGAGTTGTATTGT+TGG | 0.495681 | 4.4:+30466751 | None:intergenic |
| TTGGCAGAAAGGAAACCAAT+TGG | 0.505370 | 4.4:+30466770 | None:intergenic |
| GAGAGCGGTGAAATTTCAAA+AGG | 0.513548 | 4.4:-30467707 | MS.gene56528:CDS |
| GAGTTGTATTGTTGGCAGAA+AGG | 0.513691 | 4.4:+30466759 | None:intergenic |
| GAGCATCATCCCAAAGAAAA+GGG | 0.519493 | 4.4:-30467685 | MS.gene56528:CDS |
| AACAATGGCTGCTGCTGCTG+AGG | 0.545248 | 4.4:+30466821 | None:intergenic |
| ACCGGAAATGCGTCCGCAGA+AGG | 0.551171 | 4.4:+30466737 | None:intergenic |
| TTTGCAGCTATAAGCACACT+TGG | 0.563238 | 4.4:-30467620 | MS.gene56528:CDS |
| TTACAATGTGGAAGAATGAC+AGG | 0.568556 | 4.4:+30467356 | None:intergenic |
| CTTGGAAGTGCATTGAGTAT+GGG | 0.582334 | 4.4:-30467602 | MS.gene56528:CDS |
| AAAGAAAAGGGATGAAGAGA+GGG | 0.589149 | 4.4:-30467673 | MS.gene56528:CDS |
| ACTACCAATTCTATTGTATG+TGG | 0.592296 | 4.4:-30467400 | MS.gene56528:CDS |
| AAAGGAAACCAATTGGCACT+GGG | 0.608159 | 4.4:+30466777 | None:intergenic |
| CAAAGAAAAGGGATGAAGAG+AGG | 0.610616 | 4.4:-30467674 | MS.gene56528:CDS |
| CAACAATACAACTCCTTCTG+CGG | 0.614837 | 4.4:-30466750 | MS.gene56528:CDS |
| CTGAAATAGTCCTTACAATG+TGG | 0.618479 | 4.4:+30467344 | None:intergenic |
| TTTGAATCTAACAAAACGTG+TGG | 0.624264 | 4.4:+30467553 | None:intergenic |
| ATAGTGAGCTACATAAACAA+TGG | 0.652493 | 4.4:+30466806 | None:intergenic |
| AAGGTGAGTACAATTGAGAG+CGG | 0.725157 | 4.4:-30467722 | MS.gene56528:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCATATTTGATTAATTTC+AGG | - | chr4.4:30466943-30466962 | MS.gene56528:intron | 15.0% |
| !! | AGTGTATGATATGTTAATTA+AGG | + | chr4.4:30466905-30466924 | None:intergenic | 20.0% |
| !! | TATATATCTGTTTGGTTAAT+GGG | - | chr4.4:30467143-30467162 | MS.gene56528:intron | 20.0% |
| !! | TTATATATCTGTTTGGTTAA+TGG | - | chr4.4:30467142-30467161 | MS.gene56528:intron | 20.0% |
| !!! | CTTTAGTCTAATTTAAAACT+CGG | + | chr4.4:30467406-30467425 | None:intergenic | 20.0% |
| !!! | TTGTAATTTTAAATGACTCT+CGG | + | chr4.4:30467249-30467268 | None:intergenic | 20.0% |
| ! | AATTTATGTGCAAGTCTATA+AGG | + | chr4.4:30467299-30467318 | None:intergenic | 25.0% |
| ! | ACAAATAGCAGGAAAAAAAA+GGG | + | chr4.4:30467381-30467400 | None:intergenic | 25.0% |
| ! | ACCAAACAGATATATAACTT+TGG | + | chr4.4:30467139-30467158 | None:intergenic | 25.0% |
| ! | ATAGTGACTTACAAAAATGT+TGG | + | chr4.4:30466866-30466885 | None:intergenic | 25.0% |
| ! | TCCAAAGTTATATATCTGTT+TGG | - | chr4.4:30467135-30467154 | MS.gene56528:intron | 25.0% |
| !! | AAATTTTCGTTACTACTTAC+TGG | + | chr4.4:30467200-30467219 | None:intergenic | 25.0% |
| !! | TCTATAAGGTATCAGTTTAA+TGG | + | chr4.4:30467285-30467304 | None:intergenic | 25.0% |
| ACTACCAATTCTATTGTATG+TGG | - | chr4.4:30466975-30466994 | MS.gene56528:intron | 30.0% | |
| ATAGTGAGCTACATAAACAA+TGG | + | chr4.4:30467572-30467591 | None:intergenic | 30.0% | |
| ATGATACTTATTCTCATGTC+AGG | - | chr4.4:30467697-30467716 | MS.gene56528:CDS | 30.0% | |
| ATTGTTTATGTAGCTCACTA+TGG | - | chr4.4:30467571-30467590 | MS.gene56528:CDS | 30.0% | |
| CTAAAATGTCAAGTGTATGT+AGG | + | chr4.4:30467461-30467480 | None:intergenic | 30.0% | |
| GACAAATAGCAGGAAAAAAA+AGG | + | chr4.4:30467382-30467401 | None:intergenic | 30.0% | |
| GATTGAAATTGAACTTGTGA+AGG | - | chr4.4:30467504-30467523 | MS.gene56528:intron | 30.0% | |
| GTTTAACGACTATGTACTAA+CGG | + | chr4.4:30467359-30467378 | None:intergenic | 30.0% | |
| TGACTTACAAAAATGTTGGA+AGG | + | chr4.4:30466862-30466881 | None:intergenic | 30.0% | |
| TTTGAATCTAACAAAACGTG+TGG | + | chr4.4:30466825-30466844 | None:intergenic | 30.0% | |
| AAAGAAAAGGGATGAAGAGA+GGG | - | chr4.4:30466702-30466721 | MS.gene56528:CDS | 35.0% | |
| AGTGAAAAGTAGGATTCTCT+TGG | - | chr4.4:30467052-30467071 | MS.gene56528:intron | 35.0% | |
| CTGAAATAGTCCTTACAATG+TGG | + | chr4.4:30467034-30467053 | None:intergenic | 35.0% | |
| GAACTTGTGAAGGTTATGTT+TGG | - | chr4.4:30467514-30467533 | MS.gene56528:intron | 35.0% | |
| GGACTATTTCAGTGAAAAGT+AGG | - | chr4.4:30467042-30467061 | MS.gene56528:intron | 35.0% | |
| GTAGCCACATACAATAGAAT+TGG | + | chr4.4:30466982-30467001 | None:intergenic | 35.0% | |
| TGTCATTCTTCCACATTGTA+AGG | - | chr4.4:30467021-30467040 | MS.gene56528:intron | 35.0% | |
| TTACAATGTGGAAGAATGAC+AGG | + | chr4.4:30467022-30467041 | None:intergenic | 35.0% | |
| TTTCCAAAATTCCACCTCTA+AGG | + | chr4.4:30467434-30467453 | None:intergenic | 35.0% | |
| ! | ATGTTTGGTCTTCTTACATC+TGG | - | chr4.4:30467529-30467548 | MS.gene56528:CDS | 35.0% |
| ! | TCTCTTCATCCCTTTTCTTT+GGG | + | chr4.4:30466702-30466721 | None:intergenic | 35.0% |
| ! | TCTCTTGGTCTTTCTTGATA+CGG | - | chr4.4:30467067-30467086 | MS.gene56528:intron | 35.0% |
| !! | CTGTTTGGTTAATGGGTTAA+GGG | - | chr4.4:30467150-30467169 | MS.gene56528:intron | 35.0% |
| !! | TCTGTTTGGTTAATGGGTTA+AGG | - | chr4.4:30467149-30467168 | MS.gene56528:intron | 35.0% |
| !! | TGTTTGGTTAATGGGTTAAG+GGG | - | chr4.4:30467151-30467170 | MS.gene56528:intron | 35.0% |
| AAAGGAAACCAATTGGCACT+GGG | + | chr4.4:30467601-30467620 | None:intergenic | 40.0% | |
| AAGGTGAGTACAATTGAGAG+CGG | - | chr4.4:30466653-30466672 | MS.gene56528:CDS | 40.0% | |
| CAAAGAAAAGGGATGAAGAG+AGG | - | chr4.4:30466701-30466720 | MS.gene56528:CDS | 40.0% | |
| CAACAATACAACTCCTTCTG+CGG | - | chr4.4:30467625-30467644 | MS.gene56528:CDS | 40.0% | |
| CTAACAAAACGTGTGGCAAA+AGG | + | chr4.4:30466818-30466837 | None:intergenic | 40.0% | |
| GAAGAGAGGGCTTTCAATAA+TGG | - | chr4.4:30466715-30466734 | MS.gene56528:CDS | 40.0% | |
| GAGAGCGGTGAAATTTCAAA+AGG | - | chr4.4:30466668-30466687 | MS.gene56528:CDS | 40.0% | |
| GAGCATCATCCCAAAGAAAA+GGG | - | chr4.4:30466690-30466709 | MS.gene56528:CDS | 40.0% | |
| GAGTTGTATTGTTGGCAGAA+AGG | + | chr4.4:30467619-30467638 | None:intergenic | 40.0% | |
| TAACGACTATGTACTAACGG+TGG | + | chr4.4:30467356-30467375 | None:intergenic | 40.0% | |
| TTGGCAGAAAGGAAACCAAT+TGG | + | chr4.4:30467608-30467627 | None:intergenic | 40.0% | |
| TTTGCAGCTATAAGCACACT+TGG | - | chr4.4:30466755-30466774 | MS.gene56528:CDS | 40.0% | |
| ! | AAAACTCGGTGACAAATAGC+AGG | + | chr4.4:30467392-30467411 | None:intergenic | 40.0% |
| ! | AAAATTCCACCTCTAAGGAC+TGG | + | chr4.4:30467429-30467448 | None:intergenic | 40.0% |
| ! | ACTTGGAAGTGCATTGAGTA+TGG | - | chr4.4:30466772-30466791 | MS.gene56528:CDS | 40.0% |
| ! | AGTCCTTAGAGGTGGAATTT+TGG | - | chr4.4:30467428-30467447 | MS.gene56528:intron | 40.0% |
| ! | CTCTCTTCATCCCTTTTCTT+TGG | + | chr4.4:30466703-30466722 | None:intergenic | 40.0% |
| ! | CTTGGAAGTGCATTGAGTAT+GGG | - | chr4.4:30466773-30466792 | MS.gene56528:CDS | 40.0% |
| AGACTAAAGCCAGTCCTTAG+AGG | - | chr4.4:30467417-30467436 | MS.gene56528:intron | 45.0% | |
| ATGGAAATCCCAGTGCCAAT+TGG | - | chr4.4:30467590-30467609 | MS.gene56528:CDS | 45.0% | |
| CGCAGAAGGAGTTGTATTGT+TGG | + | chr4.4:30467627-30467646 | None:intergenic | 45.0% | |
| GAAAGGAAACCAATTGGCAC+TGG | + | chr4.4:30467602-30467621 | None:intergenic | 45.0% | |
| ! | GGAGCATCATCCCAAAGAAA+AGG | - | chr4.4:30466689-30466708 | MS.gene56528:CDS | 45.0% |
| CTAAAGCCAGTCCTTAGAGG+TGG | - | chr4.4:30467420-30467439 | MS.gene56528:intron | 50.0% | |
| GAAAGAGCCAACGAGTGAAC+CGG | + | chr4.4:30467659-30467678 | None:intergenic | 50.0% | |
| AACAATGGCTGCTGCTGCTG+AGG | + | chr4.4:30467557-30467576 | None:intergenic | 55.0% | |
| CGCATTTCCGGTTCACTCGT+TGG | - | chr4.4:30467649-30467668 | MS.gene56528:CDS | 55.0% | |
| TCCTTCTGCGGACGCATTTC+CGG | - | chr4.4:30467637-30467656 | MS.gene56528:CDS | 55.0% | |
| ACCGGAAATGCGTCCGCAGA+AGG | + | chr4.4:30467641-30467660 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 30466650 | 30467747 | 30466650 | ID=MS.gene56528 |
| chr4.4 | mRNA | 30466650 | 30467747 | 30466650 | ID=MS.gene56528.t1;Parent=MS.gene56528 |
| chr4.4 | exon | 30467524 | 30467747 | 30467524 | ID=MS.gene56528.t1.exon1;Parent=MS.gene56528.t1 |
| chr4.4 | CDS | 30467524 | 30467747 | 30467524 | ID=cds.MS.gene56528.t1;Parent=MS.gene56528.t1 |
| chr4.4 | exon | 30467309 | 30467432 | 30467309 | ID=MS.gene56528.t1.exon2;Parent=MS.gene56528.t1 |
| chr4.4 | CDS | 30467309 | 30467432 | 30467309 | ID=cds.MS.gene56528.t1;Parent=MS.gene56528.t1 |
| chr4.4 | exon | 30466650 | 30466871 | 30466650 | ID=MS.gene56528.t1.exon3;Parent=MS.gene56528.t1 |
| chr4.4 | CDS | 30466650 | 30466871 | 30466650 | ID=cds.MS.gene56528.t1;Parent=MS.gene56528.t1 |
| Gene Sequence |
| Protein sequence |
