Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056924.t1 | XP_013467946.1 | 96.9 | 194 | 6 | 0 | 1 | 194 | 1 | 194 | 5.50E-92 | 347.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056924.t1 | P0DI40 | 84.5 | 194 | 28 | 1 | 1 | 194 | 1 | 192 | 5.2e-83 | 308.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056924.t1 | A0A072VKN9 | 96.9 | 194 | 6 | 0 | 1 | 194 | 1 | 194 | 3.9e-92 | 347.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051996 | MS.gene056924 | 0.857626 | 1.44E-62 | -1.69E-46 |
| MS.gene052011 | MS.gene056924 | 0.810641 | 1.04E-50 | -1.69E-46 |
| MS.gene052223 | MS.gene056924 | 0.858386 | 8.54E-63 | -1.69E-46 |
| MS.gene054138 | MS.gene056924 | 0.816967 | 4.21E-52 | -1.69E-46 |
| MS.gene054277 | MS.gene056924 | 0.828366 | 9.36E-55 | -1.69E-46 |
| MS.gene054742 | MS.gene056924 | 0.85393 | 1.73E-61 | -1.69E-46 |
| MS.gene056330 | MS.gene056924 | 0.85975 | 3.33E-63 | -1.69E-46 |
| MS.gene056691 | MS.gene056924 | 0.82592 | 3.60E-54 | -1.69E-46 |
| MS.gene056920 | MS.gene056924 | 0.869371 | 3.26E-66 | -1.69E-46 |
| MS.gene056922 | MS.gene056924 | 0.855897 | 4.64E-62 | -1.69E-46 |
| MS.gene056923 | MS.gene056924 | 0.960468 | 1.37E-118 | -1.69E-46 |
| MS.gene056924 | MS.gene056925 | 0.923112 | 4.08E-89 | -1.69E-46 |
| MS.gene056924 | MS.gene057261 | 0.811958 | 5.39E-51 | -1.69E-46 |
| MS.gene056924 | MS.gene057544 | 0.801401 | 9.16E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene059039 | 0.808035 | 3.77E-50 | -1.69E-46 |
| MS.gene056924 | MS.gene059328 | 0.804072 | 2.57E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene060087 | 0.826538 | 2.57E-54 | -1.69E-46 |
| MS.gene056924 | MS.gene060205 | 0.812337 | 4.46E-51 | -1.69E-46 |
| MS.gene056924 | MS.gene060578 | 0.80399 | 2.68E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene063988 | 0.839042 | 2.01E-57 | -1.69E-46 |
| MS.gene056924 | MS.gene064237 | -0.823258 | 1.53E-53 | -1.69E-46 |
| MS.gene056924 | MS.gene065724 | 0.8487 | 5.19E-60 | -1.69E-46 |
| MS.gene056924 | MS.gene065728 | 0.836868 | 7.27E-57 | -1.69E-46 |
| MS.gene056924 | MS.gene065729 | 0.941264 | 5.63E-101 | -1.69E-46 |
| MS.gene056924 | MS.gene06593 | 0.854558 | 1.14E-61 | -1.69E-46 |
| MS.gene056924 | MS.gene066937 | 0.861011 | 1.38E-63 | -1.69E-46 |
| MS.gene056924 | MS.gene06756 | 0.82244 | 2.37E-53 | -1.69E-46 |
| MS.gene056924 | MS.gene06805 | 0.853952 | 1.70E-61 | -1.69E-46 |
| MS.gene056924 | MS.gene068297 | 0.80062 | 1.32E-48 | -1.69E-46 |
| MS.gene056924 | MS.gene071108 | 0.84779 | 9.25E-60 | -1.69E-46 |
| MS.gene056924 | MS.gene071840 | 0.826409 | 2.76E-54 | -1.69E-46 |
| MS.gene056924 | MS.gene07241 | 0.824284 | 8.77E-54 | -1.69E-46 |
| MS.gene056924 | MS.gene073777 | 0.813991 | 1.93E-51 | -1.69E-46 |
| MS.gene056924 | MS.gene074299 | 0.814953 | 1.19E-51 | -1.69E-46 |
| MS.gene056924 | MS.gene09768 | 0.803865 | 2.84E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene09920 | 0.80945 | 1.88E-50 | -1.69E-46 |
| MS.gene056924 | MS.gene20763 | 0.823046 | 1.71E-53 | -1.69E-46 |
| MS.gene056924 | MS.gene23026 | 0.818567 | 1.83E-52 | -1.69E-46 |
| MS.gene056924 | MS.gene23507 | 0.831134 | 1.98E-55 | -1.69E-46 |
| MS.gene056924 | MS.gene23869 | 0.846616 | 1.94E-59 | -1.69E-46 |
| MS.gene056924 | MS.gene23957 | 0.802786 | 4.76E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene24108 | 0.821857 | 3.23E-53 | -1.69E-46 |
| MS.gene056924 | MS.gene24576 | 0.808756 | 2.65E-50 | -1.69E-46 |
| MS.gene056924 | MS.gene24974 | 0.801663 | 8.10E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene25652 | 0.837113 | 6.29E-57 | -1.69E-46 |
| MS.gene056924 | MS.gene26285 | 0.82416 | 9.38E-54 | -1.69E-46 |
| MS.gene056924 | MS.gene26289 | 0.849284 | 3.57E-60 | -1.69E-46 |
| MS.gene056924 | MS.gene28548 | 0.830382 | 3.03E-55 | -1.69E-46 |
| MS.gene056924 | MS.gene30382 | 0.81342 | 2.58E-51 | -1.69E-46 |
| MS.gene056924 | MS.gene30407 | 0.800602 | 1.33E-48 | -1.69E-46 |
| MS.gene056924 | MS.gene30666 | 0.845911 | 3.02E-59 | -1.69E-46 |
| MS.gene056924 | MS.gene30911 | 0.821322 | 4.29E-53 | -1.69E-46 |
| MS.gene056924 | MS.gene31828 | 0.807816 | 4.20E-50 | -1.69E-46 |
| MS.gene056924 | MS.gene32342 | 0.833711 | 4.56E-56 | -1.69E-46 |
| MS.gene056924 | MS.gene33335 | 0.80671 | 7.21E-50 | -1.69E-46 |
| MS.gene056924 | MS.gene33992 | 0.804927 | 1.71E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene35433 | 0.853925 | 1.73E-61 | -1.69E-46 |
| MS.gene056924 | MS.gene35460 | 0.808844 | 2.54E-50 | -1.69E-46 |
| MS.gene056924 | MS.gene35690 | 0.857336 | 1.75E-62 | -1.69E-46 |
| MS.gene056924 | MS.gene35781 | 0.804748 | 1.86E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene35951 | 0.807834 | 4.16E-50 | -1.69E-46 |
| MS.gene056924 | MS.gene37233 | -0.802242 | 6.16E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene38338 | 0.810457 | 1.14E-50 | -1.69E-46 |
| MS.gene056924 | MS.gene40846 | 0.811374 | 7.23E-51 | -1.69E-46 |
| MS.gene056924 | MS.gene42005 | 0.801838 | 7.45E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene42096 | 0.832669 | 8.29E-56 | -1.69E-46 |
| MS.gene056924 | MS.gene43162 | 0.855153 | 7.64E-62 | -1.69E-46 |
| MS.gene056924 | MS.gene43874 | 0.814063 | 1.86E-51 | -1.69E-46 |
| MS.gene056924 | MS.gene44712 | 0.804043 | 2.61E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene44958 | 0.810425 | 1.16E-50 | -1.69E-46 |
| MS.gene056924 | MS.gene45463 | 0.842449 | 2.57E-58 | -1.69E-46 |
| MS.gene056924 | MS.gene45694 | 0.839934 | 1.18E-57 | -1.69E-46 |
| MS.gene056924 | MS.gene46266 | 0.802417 | 5.67E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene48169 | 0.86278 | 3.98E-64 | -1.69E-46 |
| MS.gene056924 | MS.gene50055 | 0.81243 | 4.26E-51 | -1.69E-46 |
| MS.gene056924 | MS.gene50069 | 0.842224 | 2.95E-58 | -1.69E-46 |
| MS.gene056924 | MS.gene51331 | 0.836332 | 9.96E-57 | -1.69E-46 |
| MS.gene056924 | MS.gene51389 | -0.810801 | 9.62E-51 | -1.69E-46 |
| MS.gene056924 | MS.gene54346 | 0.806627 | 7.50E-50 | -1.69E-46 |
| MS.gene056924 | MS.gene54611 | 0.864285 | 1.36E-64 | -1.69E-46 |
| MS.gene056924 | MS.gene56528 | 0.81727 | 3.60E-52 | -1.69E-46 |
| MS.gene056924 | MS.gene57807 | 0.816173 | 6.34E-52 | -1.69E-46 |
| MS.gene056924 | MS.gene58458 | 0.825893 | 3.66E-54 | -1.69E-46 |
| MS.gene056924 | MS.gene58786 | 0.811168 | 8.01E-51 | -1.69E-46 |
| MS.gene056924 | MS.gene59920 | 0.82122 | 4.53E-53 | -1.69E-46 |
| MS.gene056924 | MS.gene60581 | 0.812713 | 3.69E-51 | -1.69E-46 |
| MS.gene056924 | MS.gene61035 | 0.801176 | 1.02E-48 | -1.69E-46 |
| MS.gene056924 | MS.gene62885 | 0.80068 | 1.29E-48 | -1.69E-46 |
| MS.gene056924 | MS.gene62886 | 0.802362 | 5.82E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene64584 | 0.860849 | 1.55E-63 | -1.69E-46 |
| MS.gene056924 | MS.gene65144 | 0.848498 | 5.90E-60 | -1.69E-46 |
| MS.gene056924 | MS.gene67371 | 0.893668 | 5.07E-75 | -1.69E-46 |
| MS.gene056924 | MS.gene67377 | 0.829054 | 6.38E-55 | -1.69E-46 |
| MS.gene056924 | MS.gene67670 | 0.826944 | 2.05E-54 | -1.69E-46 |
| MS.gene056924 | MS.gene70062 | 0.814855 | 1.25E-51 | -1.69E-46 |
| MS.gene056924 | MS.gene70064 | 0.802402 | 5.71E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene71342 | 0.850586 | 1.54E-60 | -1.69E-46 |
| MS.gene056924 | MS.gene71346 | 0.839941 | 1.17E-57 | -1.69E-46 |
| MS.gene056924 | MS.gene72924 | 0.800322 | 1.52E-48 | -1.69E-46 |
| MS.gene056924 | MS.gene73993 | 0.804025 | 2.63E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene74553 | 0.810231 | 1.28E-50 | -1.69E-46 |
| MS.gene056924 | MS.gene75460 | 0.84466 | 6.59E-59 | -1.69E-46 |
| MS.gene056924 | MS.gene77006 | 0.80436 | 2.24E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene78312 | 0.805416 | 1.35E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene79232 | 0.844202 | 8.75E-59 | -1.69E-46 |
| MS.gene056924 | MS.gene80293 | 0.830277 | 3.21E-55 | -1.69E-46 |
| MS.gene056924 | MS.gene80744 | 0.818053 | 2.40E-52 | -1.69E-46 |
| MS.gene056924 | MS.gene81770 | 0.918881 | 9.01E-87 | -1.69E-46 |
| MS.gene056924 | MS.gene81771 | 0.85988 | 3.05E-63 | -1.69E-46 |
| MS.gene056924 | MS.gene81774 | 0.882812 | 7.61E-71 | -1.69E-46 |
| MS.gene056924 | MS.gene82245 | 0.803047 | 4.20E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene82532 | 0.80546 | 1.32E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene83008 | 0.814446 | 1.53E-51 | -1.69E-46 |
| MS.gene056924 | MS.gene83011 | 0.831254 | 1.85E-55 | -1.69E-46 |
| MS.gene056924 | MS.gene83366 | 0.817061 | 4.01E-52 | -1.69E-46 |
| MS.gene056924 | MS.gene84143 | 0.836459 | 9.25E-57 | -1.69E-46 |
| MS.gene056924 | MS.gene84307 | 0.804648 | 1.95E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene84373 | 0.800857 | 1.18E-48 | -1.69E-46 |
| MS.gene056924 | MS.gene85276 | 0.809292 | 2.03E-50 | -1.69E-46 |
| MS.gene056924 | MS.gene85750 | 0.878484 | 2.71E-69 | -1.69E-46 |
| MS.gene056924 | MS.gene87043 | 0.841548 | 4.44E-58 | -1.69E-46 |
| MS.gene056924 | MS.gene87222 | 0.853505 | 2.29E-61 | -1.69E-46 |
| MS.gene056924 | MS.gene87346 | 0.809042 | 2.30E-50 | -1.69E-46 |
| MS.gene056924 | MS.gene88971 | 0.830823 | 2.36E-55 | -1.69E-46 |
| MS.gene056924 | MS.gene89335 | 0.808584 | 2.88E-50 | -1.69E-46 |
| MS.gene056924 | MS.gene90814 | 0.829853 | 4.08E-55 | -1.69E-46 |
| MS.gene056924 | MS.gene91221 | 0.858661 | 7.07E-63 | -1.69E-46 |
| MS.gene056924 | MS.gene91222 | 0.848434 | 6.15E-60 | -1.69E-46 |
| MS.gene056924 | MS.gene91754 | 0.825982 | 3.48E-54 | -1.69E-46 |
| MS.gene056924 | MS.gene93200 | 0.829924 | 3.92E-55 | -1.69E-46 |
| MS.gene056924 | MS.gene95352 | 0.808014 | 3.81E-50 | -1.69E-46 |
| MS.gene056924 | MS.gene95353 | 0.804619 | 1.98E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene96782 | 0.819835 | 9.43E-53 | -1.69E-46 |
| MS.gene056924 | MS.gene97024 | 0.846208 | 2.51E-59 | -1.69E-46 |
| MS.gene056924 | MS.gene97182 | 0.864418 | 1.23E-64 | -1.69E-46 |
| MS.gene056924 | MS.gene97293 | 0.846391 | 2.24E-59 | -1.69E-46 |
| MS.gene056924 | MS.gene97335 | 0.842889 | 1.96E-58 | -1.69E-46 |
| MS.gene056924 | MS.gene97561 | 0.81962 | 1.06E-52 | -1.69E-46 |
| MS.gene056924 | MS.gene97790 | 0.814874 | 1.23E-51 | -1.69E-46 |
| MS.gene056924 | MS.gene98466 | 0.80596 | 1.04E-49 | -1.69E-46 |
| MS.gene056924 | MS.gene98470 | 0.823284 | 1.50E-53 | -1.69E-46 |
| MS.gene056924 | MS.gene99544 | 0.822235 | 2.64E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene008354 | MS.gene056924 | PPI |
| MS.gene061583 | MS.gene056924 | PPI |
| MS.gene056924 | MS.gene26200 | PPI |
| MS.gene056924 | MS.gene017305 | PPI |
| MS.gene056924 | MS.gene05033 | PPI |
| MS.gene05032 | MS.gene056924 | PPI |
| MS.gene056924 | MS.gene049677 | PPI |
| MS.gene056924 | MS.gene81804 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056924.t1 | MTR_1g058570 | 96.907 | 194 | 6 | 0 | 1 | 194 | 1 | 194 | 2.18e-136 | 379 |
| MS.gene056924.t1 | MTR_1g058540 | 79.082 | 196 | 39 | 1 | 1 | 194 | 1 | 196 | 1.02e-100 | 289 |
| MS.gene056924.t1 | MTR_1g058560 | 87.692 | 195 | 23 | 1 | 1 | 194 | 1 | 195 | 3.34e-100 | 288 |
| MS.gene056924.t1 | MTR_2g086740 | 56.818 | 176 | 76 | 0 | 18 | 193 | 44 | 219 | 1.98e-57 | 180 |
| MS.gene056924.t1 | MTR_7g011090 | 49.180 | 183 | 89 | 1 | 12 | 194 | 8 | 186 | 6.88e-55 | 172 |
| MS.gene056924.t1 | MTR_4g081880 | 43.784 | 185 | 99 | 2 | 9 | 193 | 7 | 186 | 7.07e-49 | 157 |
| MS.gene056924.t1 | MTR_5g041900 | 46.108 | 167 | 90 | 0 | 28 | 194 | 20 | 186 | 8.98e-43 | 142 |
| MS.gene056924.t1 | MTR_2g086740 | 57.944 | 107 | 45 | 0 | 18 | 124 | 44 | 150 | 1.84e-32 | 114 |
| MS.gene056924.t1 | MTR_0004s0210 | 35.849 | 159 | 89 | 4 | 31 | 187 | 3 | 150 | 2.16e-18 | 78.6 |
| MS.gene056924.t1 | MTR_7g108130 | 28.049 | 164 | 113 | 3 | 31 | 191 | 24 | 185 | 8.65e-14 | 67.0 |
| MS.gene056924.t1 | MTR_0097s0070 | 31.356 | 118 | 79 | 2 | 71 | 187 | 69 | 185 | 9.25e-13 | 64.3 |
| MS.gene056924.t1 | MTR_8g089300 | 31.013 | 158 | 96 | 4 | 40 | 187 | 30 | 184 | 1.10e-11 | 61.2 |
| MS.gene056924.t1 | MTR_3g064310 | 32.203 | 118 | 77 | 2 | 74 | 191 | 77 | 191 | 4.79e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056924.t1 | AT2G36100 | 58.911 | 202 | 74 | 1 | 2 | 194 | 5 | 206 | 3.22e-72 | 217 |
| MS.gene056924.t1 | AT5G06200 | 61.929 | 197 | 70 | 2 | 2 | 193 | 5 | 201 | 9.82e-65 | 198 |
| MS.gene056924.t1 | AT3G11550 | 61.000 | 200 | 71 | 2 | 2 | 194 | 5 | 204 | 3.59e-63 | 194 |
| MS.gene056924.t1 | AT2G27370 | 58.929 | 168 | 69 | 0 | 26 | 193 | 52 | 219 | 5.76e-61 | 189 |
| MS.gene056924.t1 | AT5G15290 | 48.000 | 150 | 78 | 0 | 25 | 174 | 17 | 166 | 1.12e-47 | 154 |
| MS.gene056924.t1 | AT1G14160 | 40.606 | 165 | 98 | 0 | 30 | 194 | 45 | 209 | 1.35e-41 | 139 |
| MS.gene056924.t1 | AT1G03700 | 32.215 | 149 | 92 | 2 | 39 | 187 | 12 | 151 | 7.01e-19 | 79.7 |
| MS.gene056924.t1 | AT4G03540 | 33.058 | 121 | 72 | 2 | 55 | 175 | 28 | 139 | 2.78e-15 | 70.5 |
| MS.gene056924.t1 | AT1G17200 | 27.545 | 167 | 109 | 4 | 29 | 191 | 23 | 181 | 3.14e-14 | 68.6 |
| MS.gene056924.t1 | AT4G15630 | 28.492 | 179 | 108 | 5 | 25 | 191 | 19 | 189 | 7.25e-14 | 67.4 |
| MS.gene056924.t1 | AT5G44550 | 30.263 | 152 | 97 | 3 | 40 | 183 | 22 | 172 | 2.26e-12 | 63.2 |
| MS.gene056924.t1 | AT3G06390 | 28.571 | 133 | 90 | 2 | 36 | 163 | 37 | 169 | 4.21e-12 | 62.8 |
Find 44 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGTAATGTTGCTTGATTC+TGG | 0.093003 | 1.4:-48639311 | None:intergenic |
| CTAGCCATTTGCAACCAATT+TGG | 0.242258 | 1.4:+48640002 | MS.gene056924:CDS |
| GTGATGTCTGCTTTGGCTCT+TGG | 0.283856 | 1.4:+48640098 | MS.gene056924:CDS |
| AAGGCCTGGAGGATGGAAAA+AGG | 0.302153 | 1.4:+48639361 | MS.gene056924:CDS |
| CCTTAGAATGAAATCCATTA+TGG | 0.309686 | 1.4:-48639390 | None:intergenic |
| CCATAATGGATTTCATTCTA+AGG | 0.312115 | 1.4:+48639390 | MS.gene056924:CDS |
| TTCCATCCTCCAGGCCTTGT+AGG | 0.319831 | 1.4:-48639356 | None:intergenic |
| TTACGATATCTAAGATAATA+AGG | 0.319981 | 1.4:-48639721 | None:intergenic |
| TGATGTCTGCTTTGGCTCTT+GGG | 0.345325 | 1.4:+48640099 | MS.gene056924:CDS |
| TATTGCCGCTGCCGCCTCAA+TGG | 0.357738 | 1.4:+48639433 | MS.gene056924:CDS |
| ATTGCCGCTGCCGCCTCAAT+GGG | 0.364904 | 1.4:+48639434 | MS.gene056924:CDS |
| TGGTAATGTTGCTTGATTCT+GGG | 0.370302 | 1.4:-48639310 | None:intergenic |
| ATGGATTTCATTCTAAGGTT+AGG | 0.379588 | 1.4:+48639395 | MS.gene056924:CDS |
| AGCTGCTTCCATAGTTTACT+TGG | 0.387801 | 1.4:+48639950 | MS.gene056924:CDS |
| GTTGGTTGTGATGTCTGCTT+TGG | 0.390211 | 1.4:+48640091 | MS.gene056924:CDS |
| GTGCACAGAAATCTCCAAAT+TGG | 0.409853 | 1.4:-48640016 | None:intergenic |
| ATGGAAATCAAGACACAAAC+TGG | 0.414877 | 1.4:+48639979 | MS.gene056924:CDS |
| AGCTGCAGCAGCACTTGAAG+TGG | 0.421850 | 1.4:-48639931 | None:intergenic |
| AAGCCTTGGTCCAGGCGCAT+GGG | 0.441787 | 1.4:-48639696 | None:intergenic |
| AAAGCCTTGGTCCAGGCGCA+TGG | 0.443522 | 1.4:-48639697 | None:intergenic |
| AGCGGCAATAGCCGCTGCTA+TGG | 0.444367 | 1.4:-48639420 | None:intergenic |
| ATCTCCAAATTGGTTGCAAA+TGG | 0.445100 | 1.4:-48640006 | None:intergenic |
| TGGTAGGGATAGAACTAAGT+AGG | 0.473587 | 1.4:-48639648 | None:intergenic |
| TGTTCCCATTGAGGCGGCAG+CGG | 0.482406 | 1.4:-48639438 | None:intergenic |
| CTAGTTGCACCTACAAGGCC+TGG | 0.489392 | 1.4:+48639347 | MS.gene056924:CDS |
| ATTGTGTGCCAAGTAAACTA+TGG | 0.493367 | 1.4:-48639958 | None:intergenic |
| GCTTGATTCTGGGACATCAA+TGG | 0.503870 | 1.4:-48639300 | None:intergenic |
| TGGCTACGACAGAGAATGGT+AGG | 0.504458 | 1.4:-48639664 | None:intergenic |
| CAATTCTAGTTGCACCTACA+AGG | 0.507515 | 1.4:+48639342 | MS.gene056924:CDS |
| CACCTACAAGGCCTGGAGGA+TGG | 0.513895 | 1.4:+48639354 | MS.gene056924:CDS |
| CTGATCACTTGTTCCCATTG+AGG | 0.525536 | 1.4:-48639447 | None:intergenic |
| AGCCTTGGTCCAGGCGCATG+GGG | 0.531806 | 1.4:-48639695 | None:intergenic |
| ATGATGGCTACGACAGAGAA+TGG | 0.540767 | 1.4:-48639668 | None:intergenic |
| ATAAGGAAAAGCCTTGGTCC+AGG | 0.552077 | 1.4:-48639704 | None:intergenic |
| AAGATAATAAGGAAAAGCCT+TGG | 0.569952 | 1.4:-48639710 | None:intergenic |
| ATCACTTGTTCCCATTGAGG+CGG | 0.575884 | 1.4:-48639444 | None:intergenic |
| AGTTGCATCATTTATTACTG+TGG | 0.594258 | 1.4:+48640055 | MS.gene056924:CDS |
| ATAGTTTACTTGGCACACAA+TGG | 0.619739 | 1.4:+48639960 | MS.gene056924:CDS |
| AAGGTTAGGTGCCATAGCAG+CGG | 0.656083 | 1.4:+48639409 | MS.gene056924:CDS |
| GGCTACGACAGAGAATGGTA+GGG | 0.661247 | 1.4:-48639663 | None:intergenic |
| GCCTTGGTCCAGGCGCATGG+GGG | 0.663208 | 1.4:-48639694 | None:intergenic |
| AGAATCAAGCAACATTACCA+AGG | 0.678912 | 1.4:+48639313 | MS.gene056924:CDS |
| GATTTCTGTGCACAGACAAG+TGG | 0.683686 | 1.4:+48640026 | MS.gene056924:CDS |
| GTTGCACCTACAAGGCCTGG+AGG | 0.707990 | 1.4:+48639350 | MS.gene056924:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGTTAATAAAAATATGATAA+GGG | + | chr1.4:48639791-48639810 | MS.gene056924:intron | 10.0% |
| !! | TTGTTAATAAAAATATGATA+AGG | + | chr1.4:48639790-48639809 | MS.gene056924:intron | 10.0% |
| !! | ATTAAATTGTATACATCAAT+CGG | - | chr1.4:48639830-48639849 | None:intergenic | 15.0% |
| !! | GTTAATAAAAATATGATAAG+GGG | + | chr1.4:48639792-48639811 | MS.gene056924:intron | 15.0% |
| !!! | GTATTAGCTTATTATAATTA+TGG | - | chr1.4:48639582-48639601 | None:intergenic | 15.0% |
| !!! | AACTGTGTTTATGAAATTTT+AGG | + | chr1.4:48639893-48639912 | MS.gene056924:intron | 20.0% |
| !!! | TTACGATATCTAAGATAATA+AGG | - | chr1.4:48639724-48639743 | None:intergenic | 20.0% |
| !!! | AACTAGAATTGTTTTTTCCT+TGG | - | chr1.4:48639333-48639352 | None:intergenic | 25.0% |
| !!! | CAGGTTTTTTGTTATTTCAA+TGG | + | chr1.4:48639613-48639632 | MS.gene056924:intron | 25.0% |
| AAGATAATAAGGAAAAGCCT+TGG | - | chr1.4:48639713-48639732 | None:intergenic | 30.0% | |
| AGTTGCATCATTTATTACTG+TGG | + | chr1.4:48640055-48640074 | MS.gene056924:CDS | 30.0% | |
| CCTTAGAATGAAATCCATTA+TGG | - | chr1.4:48639393-48639412 | None:intergenic | 30.0% | |
| GAAAGAACTGAGTGAAAAAA+GGG | - | chr1.4:48639478-48639497 | None:intergenic | 30.0% | |
| TAAAAATATGATAAGGGGTG+CGG | + | chr1.4:48639797-48639816 | MS.gene056924:intron | 30.0% | |
| TGAAAGAACTGAGTGAAAAA+AGG | - | chr1.4:48639479-48639498 | None:intergenic | 30.0% | |
| !! | ATGGATTTCATTCTAAGGTT+AGG | + | chr1.4:48639395-48639414 | MS.gene056924:CDS | 30.0% |
| !! | ATTTTAGGTGTTTCTTACTC+TGG | + | chr1.4:48639908-48639927 | MS.gene056924:intron | 30.0% |
| !! | CCATAATGGATTTCATTCTA+AGG | + | chr1.4:48639390-48639409 | MS.gene056924:CDS | 30.0% |
| !!! | AAGTTTTGAGTGTTACATAC+TGG | - | chr1.4:48639535-48639554 | None:intergenic | 30.0% |
| !!! | TGTGGTTGTTTTAATCTTGT+TGG | + | chr1.4:48640073-48640092 | MS.gene056924:CDS | 30.0% |
| AGAATCAAGCAACATTACCA+AGG | + | chr1.4:48639313-48639332 | MS.gene056924:CDS | 35.0% | |
| ATAGTTTACTTGGCACACAA+TGG | + | chr1.4:48639960-48639979 | MS.gene056924:CDS | 35.0% | |
| ATCTCCAAATTGGTTGCAAA+TGG | - | chr1.4:48640009-48640028 | None:intergenic | 35.0% | |
| ATGGAAATCAAGACACAAAC+TGG | + | chr1.4:48639979-48639998 | MS.gene056924:CDS | 35.0% | |
| ATTGTGTGCCAAGTAAACTA+TGG | - | chr1.4:48639961-48639980 | None:intergenic | 35.0% | |
| GCTAATACTTGAAACAATGC+AGG | + | chr1.4:48639594-48639613 | MS.gene056924:intron | 35.0% | |
| TTTGAGTGTTACATACTGGA+AGG | - | chr1.4:48639531-48639550 | None:intergenic | 35.0% | |
| ! | TGGTAATGTTGCTTGATTCT+GGG | - | chr1.4:48639313-48639332 | None:intergenic | 35.0% |
| !! | TTGGTAATGTTGCTTGATTC+TGG | - | chr1.4:48639314-48639333 | None:intergenic | 35.0% |
| AGCTGCTTCCATAGTTTACT+TGG | + | chr1.4:48639950-48639969 | MS.gene056924:CDS | 40.0% | |
| CTAGCCATTTGCAACCAATT+TGG | + | chr1.4:48640002-48640021 | MS.gene056924:CDS | 40.0% | |
| GAAAAAGGGAACAGCCATAA+TGG | + | chr1.4:48639376-48639395 | MS.gene056924:CDS | 40.0% | |
| GTGCACAGAAATCTCCAAAT+TGG | - | chr1.4:48640019-48640038 | None:intergenic | 40.0% | |
| TGGTAGGGATAGAACTAAGT+AGG | - | chr1.4:48639651-48639670 | None:intergenic | 40.0% | |
| ! | CAATTCTAGTTGCACCTACA+AGG | + | chr1.4:48639342-48639361 | MS.gene056924:CDS | 40.0% |
| ATAAGGAAAAGCCTTGGTCC+AGG | - | chr1.4:48639707-48639726 | None:intergenic | 45.0% | |
| ATCACTTGTTCCCATTGAGG+CGG | - | chr1.4:48639447-48639466 | None:intergenic | 45.0% | |
| ATGATGGCTACGACAGAGAA+TGG | - | chr1.4:48639671-48639690 | None:intergenic | 45.0% | |
| CTGATCACTTGTTCCCATTG+AGG | - | chr1.4:48639450-48639469 | None:intergenic | 45.0% | |
| GATTTCTGTGCACAGACAAG+TGG | + | chr1.4:48640026-48640045 | MS.gene056924:CDS | 45.0% | |
| GTTGGTTGTGATGTCTGCTT+TGG | + | chr1.4:48640091-48640110 | MS.gene056924:CDS | 45.0% | |
| !! | GCTTGATTCTGGGACATCAA+TGG | - | chr1.4:48639303-48639322 | None:intergenic | 45.0% |
| !! | TGATGTCTGCTTTGGCTCTT+GGG | + | chr1.4:48640099-48640118 | MS.gene056924:CDS | 45.0% |
| AAGGCCTGGAGGATGGAAAA+AGG | + | chr1.4:48639361-48639380 | MS.gene056924:CDS | 50.0% | |
| AGGCCTGGAGGATGGAAAAA+GGG | + | chr1.4:48639362-48639381 | MS.gene056924:CDS | 50.0% | |
| GGCTACGACAGAGAATGGTA+GGG | - | chr1.4:48639666-48639685 | None:intergenic | 50.0% | |
| TGGCTACGACAGAGAATGGT+AGG | - | chr1.4:48639667-48639686 | None:intergenic | 50.0% | |
| ! | AAGGTTAGGTGCCATAGCAG+CGG | + | chr1.4:48639409-48639428 | MS.gene056924:CDS | 50.0% |
| ! | GTTCCCTTTTTCCATCCTCC+AGG | - | chr1.4:48639368-48639387 | None:intergenic | 50.0% |
| !! | GTGATGTCTGCTTTGGCTCT+TGG | + | chr1.4:48640098-48640117 | MS.gene056924:CDS | 50.0% |
| AGCTGCAGCAGCACTTGAAG+TGG | - | chr1.4:48639934-48639953 | None:intergenic | 55.0% | |
| CTAGTTGCACCTACAAGGCC+TGG | + | chr1.4:48639347-48639366 | MS.gene056924:CDS | 55.0% | |
| ! | TTCCATCCTCCAGGCCTTGT+AGG | - | chr1.4:48639359-48639378 | None:intergenic | 55.0% |
| AAAGCCTTGGTCCAGGCGCA+TGG | - | chr1.4:48639700-48639719 | None:intergenic | 60.0% | |
| AAGCCTTGGTCCAGGCGCAT+GGG | - | chr1.4:48639699-48639718 | None:intergenic | 60.0% | |
| AGCGGCAATAGCCGCTGCTA+TGG | - | chr1.4:48639423-48639442 | None:intergenic | 60.0% | |
| ATTGCCGCTGCCGCCTCAAT+GGG | + | chr1.4:48639434-48639453 | MS.gene056924:CDS | 60.0% | |
| CACCTACAAGGCCTGGAGGA+TGG | + | chr1.4:48639354-48639373 | MS.gene056924:CDS | 60.0% | |
| GTTGCACCTACAAGGCCTGG+AGG | + | chr1.4:48639350-48639369 | MS.gene056924:CDS | 60.0% | |
| TATTGCCGCTGCCGCCTCAA+TGG | + | chr1.4:48639433-48639452 | MS.gene056924:CDS | 60.0% | |
| TGTTCCCATTGAGGCGGCAG+CGG | - | chr1.4:48639441-48639460 | None:intergenic | 60.0% | |
| AGCCTTGGTCCAGGCGCATG+GGG | - | chr1.4:48639698-48639717 | None:intergenic | 65.0% | |
| ATCATACGCCCCCATGCGCC+TGG | + | chr1.4:48639686-48639705 | MS.gene056924:CDS | 65.0% | |
| ! | AGGCGCATGGGGGCGTATGA+TGG | - | chr1.4:48639687-48639706 | None:intergenic | 65.0% |
| GCCTTGGTCCAGGCGCATGG+GGG | - | chr1.4:48639697-48639716 | None:intergenic | 70.0% | |
| GCCCCCATGCGCCTGGACCA+AGG | + | chr1.4:48639693-48639712 | MS.gene056924:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 48639290 | 48640130 | 48639290 | ID=MS.gene056924 |
| chr1.4 | mRNA | 48639290 | 48640130 | 48639290 | ID=MS.gene056924.t1;Parent=MS.gene056924 |
| chr1.4 | exon | 48639290 | 48639534 | 48639290 | ID=MS.gene056924.t1.exon1;Parent=MS.gene056924.t1 |
| chr1.4 | CDS | 48639290 | 48639534 | 48639290 | ID=cds.MS.gene056924.t1;Parent=MS.gene056924.t1 |
| chr1.4 | exon | 48639616 | 48639739 | 48639616 | ID=MS.gene056924.t1.exon2;Parent=MS.gene056924.t1 |
| chr1.4 | CDS | 48639616 | 48639739 | 48639616 | ID=cds.MS.gene056924.t1;Parent=MS.gene056924.t1 |
| chr1.4 | exon | 48639915 | 48640130 | 48639915 | ID=MS.gene056924.t1.exon3;Parent=MS.gene056924.t1 |
| chr1.4 | CDS | 48639915 | 48640130 | 48639915 | ID=cds.MS.gene056924.t1;Parent=MS.gene056924.t1 |
| Gene Sequence |
| Protein sequence |
