Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56860.t1 | KEH31820.1 | 91.5 | 329 | 28 | 0 | 283 | 611 | 24 | 352 | 1.30E-171 | 613.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56860.t1 | Q9ZRD6 | 87.4 | 183 | 23 | 0 | 1 | 183 | 1 | 183 | 1.2e-85 | 318.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56860.t1 | A0A072V130 | 91.5 | 329 | 28 | 0 | 283 | 611 | 24 | 352 | 9.3e-172 | 613.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050359 | MS.gene56860 | 0.802304 | 5.98E-49 | -1.69E-46 |
| MS.gene051003 | MS.gene56860 | 0.817281 | 3.58E-52 | -1.69E-46 |
| MS.gene054004 | MS.gene56860 | 0.808054 | 3.74E-50 | -1.69E-46 |
| MS.gene059290 | MS.gene56860 | 0.810103 | 1.36E-50 | -1.69E-46 |
| MS.gene060821 | MS.gene56860 | 0.802801 | 4.72E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56860.t1 | MTR_4g105500 | 90.663 | 332 | 31 | 0 | 280 | 611 | 21 | 352 | 0.0 | 627 |
| MS.gene56860.t1 | MTR_4g105490 | 97.326 | 187 | 5 | 0 | 1 | 187 | 1 | 187 | 7.94e-129 | 376 |
| MS.gene56860.t1 | MTR_4g105490 | 98.907 | 183 | 2 | 0 | 1 | 183 | 1 | 183 | 7.28e-128 | 374 |
| MS.gene56860.t1 | MTR_3g077490 | 56.061 | 330 | 137 | 2 | 283 | 611 | 10 | 332 | 7.06e-124 | 369 |
| MS.gene56860.t1 | MTR_4g105490 | 93.989 | 183 | 2 | 1 | 1 | 183 | 1 | 174 | 2.15e-117 | 347 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56860.t1 | AT5G58060 | 87.432 | 183 | 23 | 0 | 1 | 183 | 1 | 183 | 6.94e-111 | 331 |
| MS.gene56860.t1 | AT5G58060 | 76.585 | 205 | 26 | 1 | 1 | 183 | 1 | 205 | 7.88e-102 | 308 |
| MS.gene56860.t1 | AT5G63050 | 46.462 | 325 | 165 | 4 | 295 | 611 | 16 | 339 | 1.70e-94 | 294 |
| MS.gene56860.t1 | AT5G63050 | 46.462 | 325 | 165 | 4 | 295 | 611 | 22 | 345 | 1.76e-94 | 294 |
| MS.gene56860.t1 | AT5G58180 | 75.135 | 185 | 42 | 2 | 1 | 183 | 1 | 183 | 2.63e-89 | 275 |
| MS.gene56860.t1 | AT5G58180 | 75.135 | 185 | 42 | 2 | 1 | 183 | 1 | 183 | 2.63e-89 | 275 |
Find 117 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGCTGTTCATACAATTTA+TGG | 0.142084 | 8.1:+17510620 | MS.gene56860:CDS |
| TCAAGGAGTGGAGGAAATTT+TGG | 0.147896 | 8.1:+17509155 | MS.gene56860:CDS |
| ACTTTGTGCAGTGGGATTTA+TGG | 0.204222 | 8.1:+17499165 | MS.gene56860:CDS |
| GTTAACTCCGTTATGGGTTA+TGG | 0.250190 | 8.1:+17510661 | MS.gene56860:CDS |
| TCCTCCACTCCTTGATTTGA+TGG | 0.269774 | 8.1:-17509147 | None:intergenic |
| CGTGAGAGCTTCATTCAAAT+AGG | 0.274319 | 8.1:-17500560 | None:intergenic |
| CACTAGAGAGTGGAACATTA+TGG | 0.291342 | 8.1:-17509041 | None:intergenic |
| GATGAATATATTAAAAGTTT+TGG | 0.306961 | 8.1:+17500493 | MS.gene56860:CDS |
| CTGATTGTTCGACAATAATA+AGG | 0.324123 | 8.1:-17510971 | None:intergenic |
| AGTGGAACATTATGGGTCTT+TGG | 0.328320 | 8.1:-17509033 | None:intergenic |
| CAAGGAGTTAACTCCGTTAT+GGG | 0.331618 | 8.1:+17510655 | MS.gene56860:CDS |
| GAAGACGATGGATGAATATA+TGG | 0.337783 | 8.1:-17507090 | None:intergenic |
| CCAAGGAGTTAACTCCGTTA+TGG | 0.338317 | 8.1:+17510654 | MS.gene56860:CDS |
| TGTCACCAACATTGATTATC+AGG | 0.343112 | 8.1:+17502941 | MS.gene56860:CDS |
| TTTAAGCCTATGACGATTAC+CGG | 0.347371 | 8.1:+17508901 | MS.gene56860:CDS |
| AGGTGGTATTGATAGGTTTC+TGG | 0.348265 | 8.1:-17502873 | None:intergenic |
| AATAAAATTGTTGAAAGTAT+TGG | 0.366742 | 8.1:+17510901 | MS.gene56860:CDS |
| TTAACTCCGTTATGGGTTAT+GGG | 0.372827 | 8.1:+17510662 | MS.gene56860:CDS |
| GTGAGAGCTTCATTCAAATA+GGG | 0.385021 | 8.1:-17500559 | None:intergenic |
| TTAACAAAACATCACTTGAT+TGG | 0.390036 | 8.1:+17508200 | MS.gene56860:CDS |
| TGAATCATGGAGAAGCATTC+AGG | 0.395335 | 8.1:+17500516 | MS.gene56860:CDS |
| TTCAGGCAGGTGGTATTGAT+AGG | 0.400183 | 8.1:-17502880 | None:intergenic |
| GTTAACTCCTTGGAAACTTC+AGG | 0.401527 | 8.1:-17510644 | None:intergenic |
| AGCAACTTATCAGCCTCTGC+AGG | 0.407447 | 8.1:-17500769 | None:intergenic |
| GTACATGCTTACAACAGAAA+TGG | 0.408863 | 8.1:+17499142 | MS.gene56860:CDS |
| TTGAAGCCGGTAATCGTCAT+AGG | 0.413118 | 8.1:-17508907 | None:intergenic |
| TAAAGCAGACTACTTGTCTA+AGG | 0.414939 | 8.1:+17508246 | MS.gene56860:CDS |
| GTAGGGCCTCCTCACATTTG+TGG | 0.420972 | 8.1:+17508871 | MS.gene56860:CDS |
| AACAATGAACTCTTTAACGC+TGG | 0.424776 | 8.1:-17498641 | None:intergenic |
| CAGTGACCGGAATGATGATT+TGG | 0.431019 | 8.1:+17508933 | MS.gene56860:CDS |
| TGAAGCTCTCACGAAATTCC+AGG | 0.438362 | 8.1:+17500570 | MS.gene56860:CDS |
| AGAGAAAACGCACTACGAAC+TGG | 0.440582 | 8.1:-17499199 | None:intergenic |
| TGAATTTGCCATGGAACAAA+AGG | 0.446125 | 8.1:+17508159 | MS.gene56860:CDS |
| ACTAGAGAGTGGAACATTAT+GGG | 0.446207 | 8.1:-17509040 | None:intergenic |
| TTGTTTCGTCCAACTCCCTC+TGG | 0.449194 | 8.1:-17500798 | None:intergenic |
| CGTAGCTAATCGTACTCCTC+AGG | 0.449938 | 8.1:+17498678 | MS.gene56860:CDS |
| TGTTGAAATGCAGTGCTTCA+AGG | 0.450844 | 8.1:+17502908 | MS.gene56860:CDS |
| GACCTTCAACTCATTATCTC+TGG | 0.454124 | 8.1:-17508959 | None:intergenic |
| TGTTGAAAGTATTGGCAGAA+TGG | 0.459753 | 8.1:+17510909 | MS.gene56860:CDS |
| TGGTTACCAAATCATCATTC+CGG | 0.459887 | 8.1:-17508939 | None:intergenic |
| ATGCAGTGCTTCAAGGGACT+TGG | 0.461133 | 8.1:+17502915 | MS.gene56860:CDS |
| GAATCTTTATTGTTGCATCC+AGG | 0.467573 | 8.1:-17509275 | None:intergenic |
| GTGGGATAATTTGCAGAATG+AGG | 0.469558 | 8.1:-17510783 | None:intergenic |
| CCGGTCACTGCCTTTGAAGC+CGG | 0.481906 | 8.1:-17508920 | None:intergenic |
| TCGTGCAAGCACACTATCAA+TGG | 0.491767 | 8.1:-17501878 | None:intergenic |
| TGATTTGAGTGCAGCATCGC+AGG | 0.499499 | 8.1:+17501936 | MS.gene56860:CDS |
| ATGTAGAGAGCCACAATGAA+TGG | 0.500447 | 8.1:-17510686 | None:intergenic |
| CTTACTCACAATATTAATAG+AGG | 0.502692 | 8.1:-17509297 | None:intergenic |
| AATGAAGATCACGGCTCTGT+TGG | 0.506379 | 8.1:+17498534 | None:intergenic |
| CACCGGCGATGGTTCAGAAT+CGG | 0.512387 | 8.1:+17498567 | MS.gene56860:CDS |
| AACCAGAGATAATGAGTTGA+AGG | 0.512918 | 8.1:+17508957 | MS.gene56860:CDS |
| CTCAGTTAGAGTAGAAGAGA+AGG | 0.513887 | 8.1:+17508990 | MS.gene56860:CDS |
| AATCTTTATTGTTGCATCCA+GGG | 0.514009 | 8.1:-17509274 | None:intergenic |
| TTTGAAAAGTTTATGAACAT+CGG | 0.515580 | 8.1:-17509097 | None:intergenic |
| GTATGAACAGCAAGGAAGAA+AGG | 0.520949 | 8.1:-17510611 | None:intergenic |
| AATCGTCATAGGCTTAAACT+TGG | 0.521607 | 8.1:-17508896 | None:intergenic |
| GCTTGCACGAGGAGAGAAGC+TGG | 0.527117 | 8.1:+17501891 | MS.gene56860:CDS |
| GTTGAAATGCAGTGCTTCAA+GGG | 0.530850 | 8.1:+17502909 | MS.gene56860:CDS |
| ACTTGATTGGAAGAGCTGAC+TGG | 0.531164 | 8.1:+17508213 | MS.gene56860:CDS |
| TCCACTCCTTGATTTGATGG+TGG | 0.532232 | 8.1:-17509144 | None:intergenic |
| TGGGATAATTTGCAGAATGA+GGG | 0.537577 | 8.1:-17510782 | None:intergenic |
| ATGACGATTACCGGCTTCAA+AGG | 0.537711 | 8.1:+17508910 | MS.gene56860:CDS |
| TTATGGTCCTGAAGTTTCCA+AGG | 0.542170 | 8.1:+17510637 | MS.gene56860:CDS |
| TCGCAGCTCTCGAAATTGGA+AGG | 0.543864 | 8.1:+17507134 | MS.gene56860:CDS |
| AGAAATGGACTTTGTGCAGT+GGG | 0.544952 | 8.1:+17499157 | MS.gene56860:CDS |
| CCGGCTTCAAAGGCAGTGAC+CGG | 0.545894 | 8.1:+17508920 | MS.gene56860:CDS |
| TTTCATCGATGAATTTGCCA+TGG | 0.547002 | 8.1:+17508150 | MS.gene56860:CDS |
| CCATAACGGAGTTAACTCCT+TGG | 0.547387 | 8.1:-17510654 | None:intergenic |
| GGTTATGGGGCCATTCATTG+TGG | 0.550276 | 8.1:+17510676 | MS.gene56860:CDS |
| TCTCTACATCAAGATGGTGC+AGG | 0.555552 | 8.1:+17510700 | MS.gene56860:CDS |
| CAGAAATGGACTTTGTGCAG+TGG | 0.556323 | 8.1:+17499156 | MS.gene56860:CDS |
| AAAACGAAAATGTTGAATTG+AGG | 0.558555 | 8.1:-17507112 | None:intergenic |
| GCTTCAGATGTGAGTCACTT+CGG | 0.561337 | 8.1:+17498604 | MS.gene56860:CDS |
| TGCTCACCACCATCAAATCA+AGG | 0.564524 | 8.1:+17509138 | MS.gene56860:CDS |
| GCTGAAAATCCAGAGGGAGT+TGG | 0.565538 | 8.1:+17500789 | MS.gene56860:CDS |
| GTGCTAAAATCCACCGGCGA+TGG | 0.570937 | 8.1:+17498556 | MS.gene56860:CDS |
| GGAACACCCGTGTTTCAATA+CGG | 0.571477 | 8.1:+17507155 | MS.gene56860:CDS |
| CTTCTCTTCTACTCTAACTG+AGG | 0.578799 | 8.1:-17508989 | None:intergenic |
| CTACTCTAACTGAGGTCTTG+CGG | 0.582062 | 8.1:-17508981 | None:intergenic |
| TCAGTTAGAGTAGAAGAGAA+GGG | 0.582284 | 8.1:+17508991 | MS.gene56860:CDS |
| CACCGATTCTGAACCATCGC+CGG | 0.582685 | 8.1:-17498569 | None:intergenic |
| GCTTCATTCAAATAGGGCCA+TGG | 0.584327 | 8.1:-17500553 | None:intergenic |
| GCATCAGAGGCACTAGAGAG+TGG | 0.586775 | 8.1:-17509051 | None:intergenic |
| AATGGCCCCATAACCCATAA+CGG | 0.591221 | 8.1:-17510668 | None:intergenic |
| CAGTTAGAGTAGAAGAGAAG+GGG | 0.592260 | 8.1:+17508992 | MS.gene56860:CDS |
| AAAGTATTGGCAGAATGGAT+CGG | 0.597755 | 8.1:+17510914 | MS.gene56860:CDS |
| TGTGGCTCTCTACATCAAGA+TGG | 0.602943 | 8.1:+17510694 | MS.gene56860:CDS |
| TAACTCCGTTATGGGTTATG+GGG | 0.611008 | 8.1:+17510663 | MS.gene56860:CDS |
| GTCAGACTTTGAAGGTGGTG+TGG | 0.611347 | 8.1:+17509238 | MS.gene56860:CDS |
| GTAGCTAATCGTACTCCTCA+GGG | 0.612475 | 8.1:+17498679 | MS.gene56860:CDS |
| AAAATTCACAAGGGATGCAA+CGG | 0.616015 | 8.1:+17509205 | MS.gene56860:CDS |
| ACCATCAAATCAAGGAGTGG+AGG | 0.616892 | 8.1:+17509146 | MS.gene56860:CDS |
| AGTTAGAGTAGAAGAGAAGG+GGG | 0.619159 | 8.1:+17508993 | MS.gene56860:CDS |
| CGATTCTGAACCATCGCCGG+TGG | 0.619269 | 8.1:-17498566 | None:intergenic |
| CATAAATTGTATGAACAGCA+AGG | 0.619449 | 8.1:-17510619 | None:intergenic |
| AAATCTCCAGGACCCTGCAG+AGG | 0.620458 | 8.1:+17500756 | MS.gene56860:intron |
| CTGAGGAGTACGATTAGCTA+CGG | 0.622967 | 8.1:-17498677 | None:intergenic |
| AAGACTCTCATTAGCATCAG+AGG | 0.624192 | 8.1:-17509064 | None:intergenic |
| AGCGTACCGTATTGAAACAC+GGG | 0.625287 | 8.1:-17507161 | None:intergenic |
| CGTCAATCTGTTCAGCACGA+AGG | 0.627219 | 8.1:+17498706 | MS.gene56860:CDS |
| CTGTTGGTGCTAAAATCCAC+CGG | 0.630376 | 8.1:+17498550 | MS.gene56860:CDS |
| TGTTGAATTGAGGAAGACGA+TGG | 0.631671 | 8.1:-17507102 | None:intergenic |
| GCAACTTATCAGCCTCTGCA+GGG | 0.635798 | 8.1:-17500768 | None:intergenic |
| AGCTACCTGATAATCAATGT+TGG | 0.644611 | 8.1:-17502946 | None:intergenic |
| ACCACCATCAAATCAAGGAG+TGG | 0.644991 | 8.1:+17509143 | MS.gene56860:CDS |
| ATTGATAGTGTGCTTGCACG+AGG | 0.650465 | 8.1:+17501880 | MS.gene56860:CDS |
| ACAGATTGACGCTGACCCTG+AGG | 0.664459 | 8.1:-17498694 | None:intergenic |
| GGTCTATCGGAAAATTCACA+AGG | 0.670322 | 8.1:+17509195 | MS.gene56860:CDS |
| TAAGTTGCTGAAAATCCAGA+GGG | 0.671975 | 8.1:+17500783 | MS.gene56860:CDS |
| TTGGATCCACAAATGTGAGG+AGG | 0.674354 | 8.1:-17508877 | None:intergenic |
| AACTTGGATCCACAAATGTG+AGG | 0.678690 | 8.1:-17508880 | None:intergenic |
| TAGCGTACCGTATTGAAACA+CGG | 0.684824 | 8.1:-17507162 | None:intergenic |
| ATAAGTTGCTGAAAATCCAG+AGG | 0.687647 | 8.1:+17500782 | MS.gene56860:CDS |
| AGGCAGACAGTACTGAACCA+TGG | 0.712623 | 8.1:+17500536 | MS.gene56860:CDS |
| GTTAGAGTAGAAGAGAAGGG+GGG | 0.714547 | 8.1:+17508994 | MS.gene56860:CDS |
| GTCTATCGGAAAATTCACAA+GGG | 0.726313 | 8.1:+17509196 | MS.gene56860:CDS |
| TGAATACATCTGTTTCCACG+CGG | 0.796834 | 8.1:+17503297 | MS.gene56860:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 17498535 | 17511021 | 17498535 | ID=MS.gene56860 |
| chr8.1 | mRNA | 17498535 | 17511021 | 17498535 | ID=MS.gene56860.t1;Parent=MS.gene56860 |
| chr8.1 | exon | 17498535 | 17498727 | 17498535 | ID=MS.gene56860.t1.exon1;Parent=MS.gene56860.t1 |
| chr8.1 | CDS | 17498535 | 17498727 | 17498535 | ID=cds.MS.gene56860.t1;Parent=MS.gene56860.t1 |
| chr8.1 | exon | 17499134 | 17499231 | 17499134 | ID=MS.gene56860.t1.exon2;Parent=MS.gene56860.t1 |
| chr8.1 | CDS | 17499134 | 17499231 | 17499134 | ID=cds.MS.gene56860.t1;Parent=MS.gene56860.t1 |
| chr8.1 | exon | 17500487 | 17500591 | 17500487 | ID=MS.gene56860.t1.exon3;Parent=MS.gene56860.t1 |
| chr8.1 | CDS | 17500487 | 17500591 | 17500487 | ID=cds.MS.gene56860.t1;Parent=MS.gene56860.t1 |
| chr8.1 | exon | 17500766 | 17500831 | 17500766 | ID=MS.gene56860.t1.exon4;Parent=MS.gene56860.t1 |
| chr8.1 | CDS | 17500766 | 17500831 | 17500766 | ID=cds.MS.gene56860.t1;Parent=MS.gene56860.t1 |
| chr8.1 | exon | 17501871 | 17501957 | 17501871 | ID=MS.gene56860.t1.exon5;Parent=MS.gene56860.t1 |
| chr8.1 | CDS | 17501871 | 17501957 | 17501871 | ID=cds.MS.gene56860.t1;Parent=MS.gene56860.t1 |
| chr8.1 | exon | 17502870 | 17502962 | 17502870 | ID=MS.gene56860.t1.exon6;Parent=MS.gene56860.t1 |
| chr8.1 | CDS | 17502870 | 17502962 | 17502870 | ID=cds.MS.gene56860.t1;Parent=MS.gene56860.t1 |
| chr8.1 | exon | 17503223 | 17503318 | 17503223 | ID=MS.gene56860.t1.exon7;Parent=MS.gene56860.t1 |
| chr8.1 | CDS | 17503223 | 17503318 | 17503223 | ID=cds.MS.gene56860.t1;Parent=MS.gene56860.t1 |
| chr8.1 | exon | 17507069 | 17507176 | 17507069 | ID=MS.gene56860.t1.exon8;Parent=MS.gene56860.t1 |
| chr8.1 | CDS | 17507069 | 17507176 | 17507069 | ID=cds.MS.gene56860.t1;Parent=MS.gene56860.t1 |
| chr8.1 | exon | 17508134 | 17508267 | 17508134 | ID=MS.gene56860.t1.exon9;Parent=MS.gene56860.t1 |
| chr8.1 | CDS | 17508134 | 17508267 | 17508134 | ID=cds.MS.gene56860.t1;Parent=MS.gene56860.t1 |
| chr8.1 | exon | 17508870 | 17509310 | 17508870 | ID=MS.gene56860.t1.exon10;Parent=MS.gene56860.t1 |
| chr8.1 | CDS | 17508870 | 17509310 | 17508870 | ID=cds.MS.gene56860.t1;Parent=MS.gene56860.t1 |
| chr8.1 | exon | 17510607 | 17511021 | 17510607 | ID=MS.gene56860.t1.exon11;Parent=MS.gene56860.t1 |
| chr8.1 | CDS | 17510607 | 17511021 | 17510607 | ID=cds.MS.gene56860.t1;Parent=MS.gene56860.t1 |
| Gene Sequence |
| Protein sequence |
