AlfalfaGEDB | MS.gene059290

M. sativa cultivar XinJiangDaYe / MS.gene059290

KEGG: K09503
GO: GO:0000166 GO:0003674 GO:0005488 GO:0005515 GO:0005524 GO:0006457 GO:0006950 GO:0008144 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009987 GO:0017076 GO:0030554 GO:0031072 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0050896 GO:0051082 GO:0097159 GO:0097367 GO:1901265 GO:1901363
PFAM: PF00226.34 PF00684.22 PF01556.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059290.t1	XP_003596916.1	92.6	417	31	0	1	417	1	417	6.30E-178	633.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059290.t1	P42824	79.2	418	86	1	1	417	1	418	7.3e-159	561.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059290.t1	G7IRG5	92.6	417	31	0	1	417	1	417	4.5e-178	633.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050737	MS.gene059290	0.806065	9.86E-50	-1.69E-46
MS.gene050941	MS.gene059290	0.845907	3.03E-59	-1.69E-46
MS.gene051003	MS.gene059290	0.812592	3.92E-51	-1.69E-46
MS.gene051184	MS.gene059290	0.811007	8.68E-51	-1.69E-46
MS.gene05344	MS.gene059290	-0.802225	6.21E-49	-1.69E-46
MS.gene056849	MS.gene059290	0.800497	1.40E-48	-1.69E-46
MS.gene058278	MS.gene059290	0.813888	2.04E-51	-1.69E-46
MS.gene059290	MS.gene066221	0.829613	4.67E-55	-1.69E-46
MS.gene059290	MS.gene066427	0.812981	3.23E-51	-1.69E-46
MS.gene059290	MS.gene066840	0.812644	3.82E-51	-1.69E-46
MS.gene059290	MS.gene070671	0.832214	1.07E-55	-1.69E-46
MS.gene059290	MS.gene072541	0.8104	1.17E-50	-1.69E-46
MS.gene059290	MS.gene08889	0.807254	5.53E-50	-1.69E-46
MS.gene059290	MS.gene08922	0.803472	3.43E-49	-1.69E-46
MS.gene059290	MS.gene22695	0.814398	1.57E-51	-1.69E-46
MS.gene059290	MS.gene23876	0.805173	1.52E-49	-1.69E-46
MS.gene059290	MS.gene30352	0.807866	4.10E-50	-1.69E-46
MS.gene059290	MS.gene33363	0.804875	1.75E-49	-1.69E-46
MS.gene059290	MS.gene33364	0.8132	2.89E-51	-1.69E-46
MS.gene059290	MS.gene33365	0.808984	2.37E-50	-1.69E-46
MS.gene059290	MS.gene35034	0.804515	2.08E-49	-1.69E-46
MS.gene059290	MS.gene35518	0.917732	3.71E-86	-1.69E-46
MS.gene059290	MS.gene36311	0.814204	1.74E-51	-1.69E-46
MS.gene059290	MS.gene37988	0.871522	6.41E-67	-1.69E-46
MS.gene059290	MS.gene37989	0.862717	4.16E-64	-1.69E-46
MS.gene059290	MS.gene38261	0.844751	6.23E-59	-1.69E-46
MS.gene059290	MS.gene38262	0.816275	6.01E-52	-1.69E-46
MS.gene059290	MS.gene39143	0.80914	2.19E-50	-1.69E-46
MS.gene059290	MS.gene39874	0.824435	8.08E-54	-1.69E-46
MS.gene059290	MS.gene43152	0.804819	1.80E-49	-1.69E-46
MS.gene059290	MS.gene46305	0.803633	3.18E-49	-1.69E-46
MS.gene059290	MS.gene47674	0.802505	5.44E-49	-1.69E-46
MS.gene059290	MS.gene47818	0.80911	2.22E-50	-1.69E-46
MS.gene059290	MS.gene47995	0.822627	2.14E-53	-1.69E-46
MS.gene059290	MS.gene49477	0.840079	1.08E-57	-1.69E-46
MS.gene059290	MS.gene50200	0.813307	2.73E-51	-1.69E-46
MS.gene059290	MS.gene53070	0.82786	1.24E-54	-1.69E-46
MS.gene059290	MS.gene56860	0.810103	1.36E-50	-1.69E-46
MS.gene059290	MS.gene59207	0.826111	3.25E-54	-1.69E-46
MS.gene059290	MS.gene60372	0.816223	6.18E-52	-1.69E-46
MS.gene059290	MS.gene67008	0.811434	7.01E-51	-1.69E-46
MS.gene059290	MS.gene67010	0.818801	1.62E-52	-1.69E-46
MS.gene059290	MS.gene72933	0.803551	3.30E-49	-1.69E-46
MS.gene059290	MS.gene72994	0.802234	6.18E-49	-1.69E-46
MS.gene059290	MS.gene73462	0.801603	8.33E-49	-1.69E-46
MS.gene059290	MS.gene76635	0.824953	6.10E-54	-1.69E-46
MS.gene059290	MS.gene78867	0.802193	6.30E-49	-1.69E-46
MS.gene059290	MS.gene81285	0.831874	1.30E-55	-1.69E-46
MS.gene059290	MS.gene81288	0.816395	5.65E-52	-1.69E-46
MS.gene059290	MS.gene85109	0.817168	3.79E-52	-1.69E-46
MS.gene059290	MS.gene85111	0.805476	1.31E-49	-1.69E-46
MS.gene059290	MS.gene86919	0.805254	1.46E-49	-1.69E-46
MS.gene059290	MS.gene94952	0.807054	6.10E-50	-1.69E-46
MS.gene059290	MS.gene96690	0.80956	1.78E-50	-1.69E-46
MS.gene059290	MS.gene97281	0.838272	3.17E-57	-1.69E-46
MS.gene059290	MS.gene99505	0.804575	2.02E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene050058	MS.gene059290	PPI
MS.gene010151	MS.gene059290	PPI
MS.gene042467	MS.gene059290	PPI
MS.gene02219	MS.gene059290	PPI
MS.gene64395	MS.gene059290	PPI
MS.gene75493	MS.gene059290	PPI
MS.gene038868	MS.gene059290	PPI
MS.gene35518	MS.gene059290	PPI
MS.gene01209	MS.gene059290	PPI
MS.gene032180	MS.gene059290	PPI
MS.gene059290	MS.gene043886	PPI
MS.gene07038	MS.gene059290	PPI
MS.gene89158	MS.gene059290	PPI
MS.gene002235	MS.gene059290	PPI
MS.gene01238	MS.gene059290	PPI
MS.gene25092	MS.gene059290	PPI
MS.gene64132	MS.gene059290	PPI
MS.gene90259	MS.gene059290	PPI
MS.gene074451	MS.gene059290	PPI
MS.gene059290	MS.gene034372	PPI
MS.gene025674	MS.gene059290	PPI
MS.gene028516	MS.gene059290	PPI
MS.gene067859	MS.gene059290	PPI
MS.gene052188	MS.gene059290	PPI
MS.gene045133	MS.gene059290	PPI
MS.gene051082	MS.gene059290	PPI
MS.gene059290	MS.gene83326	PPI
MS.gene052009	MS.gene059290	PPI
MS.gene002262	MS.gene059290	PPI
MS.gene034372	MS.gene059290	PPI
MS.gene08162	MS.gene059290	PPI
MS.gene41778	MS.gene059290	PPI
MS.gene053617	MS.gene059290	PPI
MS.gene28798	MS.gene059290	PPI
MS.gene072709	MS.gene059290	PPI
MS.gene40086	MS.gene059290	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059290.t1	MTR_2g087660	99.520	417	2	0	1	417	1	417	0.0	858
MS.gene059290.t1	MTR_4g050420	91.169	419	32	3	1	417	1	416	0.0	686
MS.gene059290.t1	MTR_2g087660	98.726	314	4	0	1	314	1	314	0.0	639
MS.gene059290.t1	MTR_8g006430	70.991	424	115	5	1	417	1	423	0.0	582
MS.gene059290.t1	MTR_7g109340	37.322	351	180	9	14	358	27	343	2.80e-58	194
MS.gene059290.t1	MTR_4g078850	33.333	360	204	10	14	358	5	343	9.01e-51	174
MS.gene059290.t1	MTR_2g104030	32.143	364	221	11	11	357	68	422	3.68e-49	174
MS.gene059290.t1	MTR_4g023470	34.463	354	186	11	14	347	87	414	1.60e-48	171
MS.gene059290.t1	MTR_3g087030	33.144	353	217	9	14	357	83	425	3.15e-48	170
MS.gene059290.t1	MTR_2g090705	32.234	394	154	9	14	358	5	334	3.02e-47	165
MS.gene059290.t1	MTR_2g014470	33.333	360	199	11	14	358	5	338	4.81e-47	164
MS.gene059290.t1	MTR_3g108020	32.213	357	194	7	14	358	5	325	5.63e-46	161
MS.gene059290.t1	MTR_2g014480	34.018	341	199	8	14	343	5	330	2.07e-45	160
MS.gene059290.t1	MTR_4g015840	30.151	398	161	9	14	358	5	338	1.01e-39	145
MS.gene059290.t1	MTR_3g027580	30.645	372	212	15	1	355	80	422	5.67e-39	145
MS.gene059290.t1	MTR_6g471050	30.792	341	210	11	41	365	9	339	1.10e-37	142
MS.gene059290.t1	MTR_5g065160	27.660	376	225	11	1	355	70	419	6.33e-32	126
MS.gene059290.t1	MTR_5g065160	27.957	372	221	11	5	355	75	420	2.28e-31	124
MS.gene059290.t1	MTR_5g065160	27.957	372	221	11	5	355	68	413	3.72e-31	124
MS.gene059290.t1	MTR_4g023490	31.781	365	209	14	14	358	17	361	5.04e-31	122
MS.gene059290.t1	MTR_5g065160	27.957	372	221	11	5	355	75	420	5.36e-31	124
MS.gene059290.t1	MTR_5g037880	36.552	145	90	1	214	358	132	274	7.79e-28	111
MS.gene059290.t1	MTR_5g037880	32.215	149	63	5	11	146	3	126	7.92e-11	62.8
MS.gene059290.t1	MTR_4g015840	36.232	138	86	1	221	358	126	261	1.08e-24	102
MS.gene059290.t1	MTR_3g437840	34.752	141	90	1	218	358	23	161	4.21e-23	95.5
MS.gene059290.t1	MTR_3g088140	34.483	145	92	2	215	358	211	353	8.31e-19	87.4
MS.gene059290.t1	MTR_2g006890	32.639	144	96	1	215	358	233	375	8.77e-18	84.7
MS.gene059290.t1	MTR_3g437850	30.723	166	78	5	14	147	3	163	2.60e-16	76.6
MS.gene059290.t1	MTR_8g037810	46.392	97	43	3	12	104	5	96	3.18e-15	76.6
MS.gene059290.t1	MTR_1g029460	43.878	98	44	4	12	104	5	96	3.39e-14	74.3
MS.gene059290.t1	MTR_5g012530	43.750	80	38	1	10	82	9	88	4.81e-14	74.3
MS.gene059290.t1	MTR_7g101070	52.564	78	31	2	4	76	349	425	5.06e-14	73.9
MS.gene059290.t1	MTR_1g110310	52.941	68	28	1	15	78	18	85	1.07e-13	72.8
MS.gene059290.t1	MTR_1g029470	43.878	98	44	4	12	104	5	96	2.80e-13	71.2
MS.gene059290.t1	MTR_5g004570	40.206	97	49	3	12	104	5	96	1.26e-12	67.8
MS.gene059290.t1	MTR_3g437360	30.534	131	88	3	210	339	206	334	2.89e-12	67.8
MS.gene059290.t1	MTR_5g035770	37.255	102	50	3	15	105	27	125	1.49e-11	66.2
MS.gene059290.t1	MTR_7g112360	40.000	75	37	1	14	80	17	91	4.74e-11	61.6
MS.gene059290.t1	MTR_7g112360	40.000	75	37	1	14	80	17	91	6.38e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059290.t1	AT5G22060	83.055	419	69	2	1	417	1	419	0.0	684
MS.gene059290.t1	AT3G44110	85.036	421	58	3	1	417	1	420	0.0	655
MS.gene059290.t1	AT3G44110	87.651	332	39	2	1	331	1	331	0.0	530
MS.gene059290.t1	AT3G62600	35.775	355	182	11	14	358	27	345	7.55e-56	188
MS.gene059290.t1	AT1G80030	32.964	361	216	10	14	357	76	427	5.33e-53	184
MS.gene059290.t1	AT1G80030	32.964	361	216	10	14	357	76	427	5.33e-53	184
MS.gene059290.t1	AT1G80030	32.964	361	216	10	14	357	76	427	5.33e-53	184
MS.gene059290.t1	AT1G80030	32.964	361	216	10	14	357	76	427	6.73e-53	184
MS.gene059290.t1	AT2G20560	33.711	353	205	8	14	358	5	336	8.01e-50	172
MS.gene059290.t1	AT5G25530	32.597	362	198	9	14	358	5	337	9.08e-48	166
MS.gene059290.t1	AT5G48030	34.167	360	201	13	14	355	95	436	1.02e-47	169
MS.gene059290.t1	AT2G22360	32.075	371	229	10	14	378	87	440	5.65e-47	167
MS.gene059290.t1	AT4G39960	31.198	359	228	9	11	357	83	434	5.94e-45	162
MS.gene059290.t1	AT4G39960	31.198	359	228	9	11	357	83	434	5.94e-45	162
MS.gene059290.t1	AT5G25530	31.892	370	198	10	14	358	5	345	6.69e-45	159
MS.gene059290.t1	AT4G28480	31.198	359	217	8	14	358	5	347	1.25e-44	158
MS.gene059290.t1	AT3G08910	30.789	380	164	9	14	358	5	320	9.36e-40	145
MS.gene059290.t1	AT3G17830	30.303	363	225	10	12	355	62	415	5.31e-38	144
MS.gene059290.t1	AT5G01390	29.517	393	165	11	14	358	5	333	9.13e-38	140
MS.gene059290.t1	AT1G28210	27.895	380	229	11	7	368	42	394	1.06e-33	130
MS.gene059290.t1	AT1G28210	27.895	380	229	11	7	368	42	394	1.48e-33	130
MS.gene059290.t1	AT1G28210	27.895	380	229	11	7	368	42	394	1.81e-33	130
MS.gene059290.t1	AT2G20550	37.241	145	89	1	214	358	141	283	1.05e-26	108
MS.gene059290.t1	AT2G20550	37.241	145	89	1	214	358	141	283	1.05e-26	108
MS.gene059290.t1	AT3G47940	36.552	145	90	1	214	358	204	346	1.79e-26	109
MS.gene059290.t1	AT3G47940	34.021	194	67	4	14	146	5	198	1.11e-20	93.2
MS.gene059290.t1	AT5G01390	35.507	138	87	1	221	358	140	275	1.09e-23	100
MS.gene059290.t1	AT5G01390	40.741	108	43	3	14	100	5	112	1.77e-16	79.7
MS.gene059290.t1	AT4G28480	34.694	147	92	2	212	358	147	289	1.66e-23	100
MS.gene059290.t1	AT4G28480	43.750	112	50	2	14	120	5	108	5.99e-18	84.3
MS.gene059290.t1	AT1G59725	36.957	138	85	1	221	358	193	328	7.36e-23	99.0
MS.gene059290.t1	AT1G59725	32.955	176	75	4	14	146	5	180	4.28e-19	88.2
MS.gene059290.t1	AT1G10350	36.552	145	87	3	214	358	207	346	7.85e-23	99.4
MS.gene059290.t1	AT1G10350	32.308	195	70	6	14	146	5	199	3.66e-19	88.6
MS.gene059290.t1	AT5G01390	35.870	184	70	6	14	149	5	188	7.41e-22	94.0
MS.gene059290.t1	AT1G11040	37.500	136	83	1	215	350	291	424	3.99e-20	92.4
MS.gene059290.t1	AT1G44160	32.593	135	89	1	224	358	221	353	1.69e-17	84.0
MS.gene059290.t1	AT5G01390	40.741	108	43	3	14	100	5	112	9.65e-17	79.7
MS.gene059290.t1	AT2G21510	44.330	97	45	3	12	104	5	96	1.78e-15	77.4
MS.gene059290.t1	AT2G21510	44.330	97	45	3	12	104	5	96	2.48e-15	77.4
MS.gene059290.t1	AT2G21510	44.330	97	45	3	12	104	5	96	2.48e-15	77.4
MS.gene059290.t1	AT2G21510	44.330	97	45	3	12	104	5	96	2.55e-15	77.4
MS.gene059290.t1	AT2G21510	44.330	97	45	3	12	104	5	96	2.55e-15	77.4
MS.gene059290.t1	AT5G05750	41.748	103	46	3	14	102	115	217	4.24e-14	72.8
MS.gene059290.t1	AT4G39150	42.268	97	47	3	12	104	5	96	9.40e-14	72.4
MS.gene059290.t1	AT4G39150	42.268	97	47	3	12	104	5	96	9.40e-14	72.4
MS.gene059290.t1	AT4G39150	42.268	97	47	3	12	104	5	96	9.40e-14	72.4
MS.gene059290.t1	AT1G59980	47.059	68	32	1	15	78	25	92	4.21e-13	70.5
MS.gene059290.t1	AT1G59980	47.059	68	32	1	15	78	25	92	6.33e-13	70.5
MS.gene059290.t1	AT5G03160	50.000	94	40	4	4	92	361	452	1.17e-12	69.7
MS.gene059290.t1	AT1G68370	51.471	68	29	1	15	78	19	86	1.24e-12	69.7
MS.gene059290.t1	AT3G57340	42.424	99	42	3	14	97	114	212	1.44e-12	68.9
MS.gene059290.t1	AT3G57340	42.424	99	42	3	14	97	114	212	1.44e-12	68.9
MS.gene059290.t1	AT3G08970	47.297	74	36	1	15	85	29	102	1.72e-12	69.7
MS.gene059290.t1	AT3G08970	47.297	74	36	1	15	85	29	102	1.72e-12	69.7
MS.gene059290.t1	AT1G24120	37.255	102	50	4	15	105	22	120	3.92e-12	68.2
MS.gene059290.t1	AT3G12170	46.479	71	34	1	8	74	42	112	1.25e-11	65.5
MS.gene059290.t1	AT3G12170	31.707	164	84	6	8	166	6	146	1.25e-11	65.1
MS.gene059290.t1	AT1G21080	39.175	97	49	3	12	104	5	95	2.06e-11	65.9
MS.gene059290.t1	AT1G21080	39.175	97	49	3	12	104	5	95	2.18e-11	65.5
MS.gene059290.t1	AT2G35720	38.824	85	45	1	5	82	7	91	2.74e-11	65.9
MS.gene059290.t1	AT1G74250	44.928	69	29	2	14	73	12	80	3.77e-11	65.5
MS.gene059290.t1	AT5G49060	46.269	67	33	1	11	74	97	163	3.98e-11	64.7
MS.gene059290.t1	AT5G49060	46.269	67	33	1	11	74	97	163	3.98e-11	64.7
MS.gene059290.t1	AT1G76700	39.394	99	51	3	12	106	5	98	6.16e-11	64.3
MS.gene059290.t1	AT4G10130	43.939	66	30	1	14	72	12	77	6.19e-11	61.6
MS.gene059290.t1	AT4G10130	43.939	66	30	1	14	72	12	77	6.19e-11	61.6
MS.gene059290.t1	AT4G10130	43.939	66	30	1	14	72	12	77	6.19e-11	61.6
MS.gene059290.t1	AT4G10130	43.939	66	30	1	14	72	12	77	6.19e-11	61.6
MS.gene059290.t1	AT4G10130	43.939	66	30	1	14	72	12	77	6.19e-11	61.6
MS.gene059290.t1	AT1G56300	41.176	68	32	1	12	71	12	79	7.82e-11	60.8
MS.gene059290.t1	AT5G06910	45.714	70	34	1	9	74	25	94	9.17e-11	62.8

Find 119 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCGGTGGAGGTGGATTTCC+TGG	0.267655	2.2:-11476038	MS.gene059290:intron
TCACCCCTTGAAGGTCTCTT+TGG	0.287702	2.2:-11475866	MS.gene059290:CDS
CCAATTTGCGCTGACTCATT+TGG	0.293928	2.2:-11475197	MS.gene059290:CDS
GGAATGGGTGGTGGCGGTGG+TGG	0.294243	2.2:-11476092	MS.gene059290:CDS
TCAGGATCACCACCCTTATC+AGG	0.295880	2.2:+11476838	None:intergenic
CCAAATGAGTCAGCGCAAAT+TGG	0.320936	2.2:+11475197	None:intergenic
TTCACCCTTTCGTTTGAACT+TGG	0.337178	2.2:+11475270	None:intergenic
GAAGATATCTATGAGGCATT+TGG	0.338997	2.2:-11475628	MS.gene059290:CDS
GAAGGAATGGGTGGTGGCGG+TGG	0.340853	2.2:-11476095	MS.gene059290:CDS
ACTAAATACTATGAGATTCT+TGG	0.343941	2.2:-11476928	MS.gene059290:CDS
AACTGAGATACAGGCCTTGC+TGG	0.344090	2.2:+11474877	None:intergenic
CCCCTTGAAGGTCTCTTTGG+AGG	0.348117	2.2:-11475863	MS.gene059290:CDS
TTGCAGCCGGACACAGTTAC+TGG	0.350028	2.2:-11475328	MS.gene059290:intron
CACCACCCATTCCTTCCTTA+AGG	0.353366	2.2:+11476102	None:intergenic
TTAGTGTTATCACTCTTCTT+AGG	0.362823	2.2:+11476946	None:intergenic
TGTGCTGGTTGTCAAGGTTC+TGG	0.368082	2.2:-11475654	MS.gene059290:CDS
TCTTGATCAGGTCAAGGCTT+TGG	0.379949	2.2:-11474911	MS.gene059290:CDS
TTGCATTTGCTGAATCATAT+TGG	0.385235	2.2:+11475602	None:intergenic
ACCACCCATTCCTTCCTTAA+GGG	0.391516	2.2:+11476103	None:intergenic
TACGCTTTCTTCAAATCATC+GGG	0.394989	2.2:+11476886	None:intergenic
CACCCTTTCGTTTGAACTTG+GGG	0.398325	2.2:+11475272	None:intergenic
TCACCCTTTCGTTTGAACTT+GGG	0.404206	2.2:+11475271	None:intergenic
AAGATATCTATGAGGCATTT+GGG	0.411623	2.2:-11475627	MS.gene059290:CDS
ATGAAAGGCCTCTGGTACAT+TGG	0.411721	2.2:+11474985	None:intergenic
TTGTGAAATTTGAAGATGTT+TGG	0.412460	2.2:-11476976	None:intergenic
TGAGTCTCTGTCTCTTGATC+AGG	0.415051	2.2:-11474923	MS.gene059290:CDS
ACATCCTTGCAATGAATGTA+AGG	0.418761	2.2:-11475580	MS.gene059290:CDS
CTTTGGAGGATCTTTACCTT+GGG	0.422824	2.2:-11475849	MS.gene059290:CDS
AGAGCTTGCACAAGCTTATG+AGG	0.426543	2.2:-11476177	MS.gene059290:CDS
ATGTCTGTCAACTGAGATAC+AGG	0.432545	2.2:+11474868	None:intergenic
AAAGATGAAAAGATGTCAAA+TGG	0.433471	2.2:+11476062	None:intergenic
CCTTCTCTCCCTTGCACTGT+GGG	0.438316	2.2:+11475523	None:intergenic
TCATATACCTCACGCTTCTC+AGG	0.445588	2.2:+11476143	None:intergenic
TCCCTCACTGAGGCTTTATG+TGG	0.451630	2.2:-11475223	MS.gene059290:CDS
TGCAATGAATGTAAGGGTAC+TGG	0.456888	2.2:-11475573	MS.gene059290:CDS
TCATCTTTCTTCGGTGGAGG+TGG	0.461355	2.2:-11476047	MS.gene059290:CDS
GTATGGTGAAGATGCCCTTA+AGG	0.462316	2.2:-11476117	MS.gene059290:CDS
GGTTTGATTTAATCAATAGT+TGG	0.465413	2.2:+11475167	None:intergenic
ATACGCTTTCTTCAAATCAT+CGG	0.468985	2.2:+11476885	None:intergenic
CGTGAGGTATATGATCAGTA+TGG	0.471347	2.2:-11476134	MS.gene059290:CDS
TCTTTGGAGGATCTTTACCT+TGG	0.472410	2.2:-11475850	MS.gene059290:CDS
GATTAAGAATCATCCTGATA+AGG	0.476346	2.2:-11476851	MS.gene059290:CDS
GGATGTCGTTCACCCCTTGA+AGG	0.478745	2.2:-11475875	MS.gene059290:CDS
AGATCGATGCCCACAGTGCA+AGG	0.481792	2.2:-11475532	MS.gene059290:CDS
ATTAAGAATCATCCTGATAA+GGG	0.485570	2.2:-11476850	MS.gene059290:CDS
TACAGGAAAGGATCAAAGTC+TGG	0.495336	2.2:-11475690	MS.gene059290:intron
TGCCCTTCATGAAAGGCCTC+TGG	0.498481	2.2:+11474977	None:intergenic
CCAGGCTTGACAACTTCTCC+AGG	0.499453	2.2:+11475145	None:intergenic
ATATCTCCAGTAACTGTGTC+CGG	0.501009	2.2:+11475322	None:intergenic
GGTGCTTCGATGACCTGTGC+TGG	0.505989	2.2:-11475669	MS.gene059290:CDS
CTTGAAGTTCATGTTGAGAA+GGG	0.510818	2.2:-11475480	MS.gene059290:CDS
GCATCGATCTTTGTCACTGA+TGG	0.515086	2.2:+11475545	None:intergenic
ATCCTCCAAAGAGACCTTCA+AGG	0.518323	2.2:+11475861	None:intergenic
AAGGAAGGAATGGGTGGTGG+CGG	0.520461	2.2:-11476098	MS.gene059290:CDS
TACAAGGCAATCAATGATGA+AGG	0.521364	2.2:-11475012	MS.gene059290:CDS
CACCCCAAGTTCAAACGAAA+GGG	0.528668	2.2:-11475274	MS.gene059290:CDS
AGATGCCCTTAAGGAAGGAA+TGG	0.532671	2.2:-11476108	MS.gene059290:CDS
TGATCAAGAGACAGAGACTC+AGG	0.533617	2.2:+11474925	None:intergenic
CCTGGAGAAGTTGTCAAGCC+TGG	0.534032	2.2:-11475145	MS.gene059290:intron
GGTCAGAAGATTACCTTCCC+TGG	0.534089	2.2:-11475447	MS.gene059290:CDS
TTCATCAGCTTCACCAGGGA+AGG	0.534704	2.2:+11475434	None:intergenic
TGGTTATGCAGATTCATACA+AGG	0.537406	2.2:-11475028	MS.gene059290:intron
TAACACATACCCCTTGCACT+TGG	0.540192	2.2:+11475786	None:intergenic
ACTTGAAGTTCATGTTGAGA+AGG	0.543623	2.2:-11475481	MS.gene059290:CDS
CTTAAGGAAGGAATGGGTGG+TGG	0.547958	2.2:-11476101	MS.gene059290:CDS
TGTCTTGTGCTCCAAGTGCA+AGG	0.549478	2.2:-11475797	MS.gene059290:CDS
TACCAGAGGCCTTTCATGAA+GGG	0.550239	2.2:-11474979	MS.gene059290:CDS
TCTGTCTCTTGATCAGGTCA+AGG	0.553837	2.2:-11474917	MS.gene059290:CDS
GATGCCCTTAAGGAAGGAAT+GGG	0.556456	2.2:-11476107	MS.gene059290:CDS
CCCACAGTGCAAGGGAGAGA+AGG	0.557002	2.2:-11475523	MS.gene059290:CDS
CGTCTTTGTACTTCAACAGA+AGG	0.558455	2.2:-11475299	MS.gene059290:CDS
TTGAAGAAGAGACAAGAAGA+AGG	0.558667	2.2:-11474801	MS.gene059290:CDS
TAAAGCTTGCCCTTCATGAA+AGG	0.559348	2.2:+11474970	None:intergenic
GTCTTGTGCTCCAAGTGCAA+GGG	0.560067	2.2:-11475796	MS.gene059290:CDS
ACCGCTTCATCAGCTTCACC+AGG	0.560539	2.2:+11475429	None:intergenic
ATGACCTGTGCTGGTTGTCA+AGG	0.561075	2.2:-11475660	MS.gene059290:CDS
GTTGAGAAGGGGATGCAGAA+TGG	0.563375	2.2:-11475468	MS.gene059290:CDS
GTACCAGAGGCCTTTCATGA+AGG	0.566111	2.2:-11474980	MS.gene059290:CDS
ACTGAGATACAGGCCTTGCT+GGG	0.567469	2.2:+11474878	None:intergenic
GGTGAAGATGCCCTTAAGGA+AGG	0.569050	2.2:-11476113	MS.gene059290:CDS
TCAGTTGACAGACATGGAGC+TGG	0.570239	2.2:-11474860	MS.gene059290:CDS
GTGGTAGTAGCAGAGGAAGA+AGG	0.572018	2.2:-11475912	MS.gene059290:CDS
ACAGGTGGTGGTAGTAGCAG+AGG	0.575959	2.2:-11475919	MS.gene059290:intron
CCCTGGTGAAGCTGATGAAG+CGG	0.582550	2.2:-11475430	MS.gene059290:intron
AGAACCTTGACAACCAGCAC+AGG	0.585983	2.2:+11475656	None:intergenic
TGGAGACAATTCTCCCAGCA+AGG	0.587524	2.2:-11474891	MS.gene059290:CDS
ACACCCCAAGTTCAAACGAA+AGG	0.592574	2.2:-11475275	MS.gene059290:CDS
TGTACTCTTTGAGCACCACC+AGG	0.594168	2.2:+11474736	None:intergenic
TCCTCCAAAGAGACCTTCAA+GGG	0.596017	2.2:+11475862	None:intergenic
TTGAAGTTCATGTTGAGAAG+GGG	0.603095	2.2:-11475479	MS.gene059290:CDS
GTACCCTTACATTCATTGCA+AGG	0.605875	2.2:+11475576	None:intergenic
CAGGCTTGACAACTTCTCCA+GGG	0.612975	2.2:+11475146	None:intergenic
TAAGGGTGGTGATCCTGAAA+AGG	0.613088	2.2:-11476833	MS.gene059290:intron
TGTATCTCAGTTGACAGACA+TGG	0.616220	2.2:-11474866	MS.gene059290:CDS
GCCCTTAAGGAAGGAATGGG+TGG	0.620209	2.2:-11476104	MS.gene059290:CDS
GATGAGGATGATGAAATGCC+TGG	0.624859	2.2:-11474754	MS.gene059290:CDS
AAGGCAGCAAGCTCAGCAAG+AGG	0.624868	2.2:-11474782	MS.gene059290:CDS
ACCTTCTCTCCCTTGCACTG+TGG	0.630501	2.2:+11475522	None:intergenic
GATCGATGCCCACAGTGCAA+GGG	0.633588	2.2:-11475531	MS.gene059290:CDS
AAAGCTTCTTAGATGTCCCA+AGG	0.634241	2.2:+11475833	None:intergenic
TCTTGTGCTCCAAGTGCAAG+GGG	0.635966	2.2:-11475795	MS.gene059290:intron
CTATTGATTAAATCAAACCC+TGG	0.637960	2.2:-11475163	MS.gene059290:CDS
AGAGGAAGAAGGCAGAGACG+TGG	0.638556	2.2:-11475901	MS.gene059290:CDS
CATCCTTGCAATGAATGTAA+GGG	0.643345	2.2:-11475579	MS.gene059290:CDS
AAGAAGGCAGAGACGTGGTG+AGG	0.643979	2.2:-11475896	MS.gene059290:CDS
TCAGCAAGAGGCATATGATG+AGG	0.654664	2.2:-11474770	MS.gene059290:CDS
ACCACATAAAGCCTCAGTGA+GGG	0.659830	2.2:+11475222	None:intergenic
CATGGAGCTGGATGAGTGTG+AGG	0.667513	2.2:-11474848	MS.gene059290:CDS
GATCAAGAGACAGAGACTCA+GGG	0.667543	2.2:+11474926	None:intergenic
AAGGAATGCCAATGTACCAG+AGG	0.667557	2.2:-11474993	MS.gene059290:CDS
CTGGTATGAAGATATCTATG+AGG	0.673684	2.2:-11475635	MS.gene059290:CDS
GAGTGATCCTGAGAAGCGTG+AGG	0.676105	2.2:-11476150	MS.gene059290:CDS
AAGAATCATCCTGATAAGGG+TGG	0.677133	2.2:-11476847	MS.gene059290:CDS
CCGCTTCATCAGCTTCACCA+GGG	0.690995	2.2:+11475430	None:intergenic
GCTTTACATTCACTTCACTG+TGG	0.705179	2.2:-11474953	MS.gene059290:CDS
CCTCCAAAGAGACCTTCAAG+GGG	0.727234	2.2:+11475863	None:intergenic
AACCACATAAAGCCTCAGTG+AGG	0.740190	2.2:+11475221	None:intergenic
GCACACATTGTCCCTCACTG+AGG	0.763892	2.2:-11475233	MS.gene059290:CDS
GAGGATGATGAAATGCCTGG+TGG	0.804018	2.2:-11474751	MS.gene059290:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTTGAGATTTTTAAATTA+AGG	-	chr2.2:11475069-11475088	MS.gene059290:intron	10.0%
!!!	ATTTGAGATTTTTAAATTAA+GGG	-	chr2.2:11475070-11475089	MS.gene059290:intron	10.0%
!!	ATTAGTTAATTGATGAAATT+AGG	-	chr2.2:11475444-11475463	MS.gene059290:CDS	15.0%
!!	ATTGAATTATATAATTGTTG+TGG	-	chr2.2:11475271-11475290	MS.gene059290:CDS	15.0%
!!	TTAGTTAATTGATGAAATTA+GGG	-	chr2.2:11475445-11475464	MS.gene059290:CDS	15.0%
!!	TTATCTTTGAATTTGATTAT+TGG	-	chr2.2:11474880-11474899	MS.gene059290:CDS	15.0%
!!!	AAAAGTGATTTTTTTACTAT+AGG	-	chr2.2:11475111-11475130	MS.gene059290:intron	15.0%
!!!	CTTGTTTGTTATTTTAATTT+AGG	-	chr2.2:11475674-11475693	MS.gene059290:CDS	15.0%
!!!	TATTTTAATTTAGGAGATTA+GGG	-	chr2.2:11475683-11475702	MS.gene059290:CDS	15.0%
!!!	TTATTTTAATTTAGGAGATT+AGG	-	chr2.2:11475682-11475701	MS.gene059290:CDS	15.0%
!!!	TTTGAGATTTTTAAATTAAG+GGG	-	chr2.2:11475071-11475090	MS.gene059290:intron	15.0%
!!!	AATAGTGATTAGTTGAATTT+AGG	-	chr2.2:11474971-11474990	MS.gene059290:CDS	20.0%
!!!	AATTGTGATTAGTTAGTTTT+AGG	-	chr2.2:11475364-11475383	MS.gene059290:intron	20.0%
!!!	ATAAAAAAAACAACCTTTTC+AGG	+	chr2.2:11474864-11474883	None:intergenic	20.0%
!!!	ATAGTGATTAGTTGAATTTA+GGG	-	chr2.2:11474972-11474991	MS.gene059290:CDS	20.0%
!!!	ATTGAATTTGATAATTGTTG+TGG	-	chr2.2:11474944-11474963	MS.gene059290:CDS	20.0%
!!!	ATTGTGATTAGTTAGTTTTA+GGG	-	chr2.2:11475365-11475384	MS.gene059290:intron	20.0%
!!!	ATTGTTAGTTGTATTGATAT+TGG	-	chr2.2:11475319-11475338	MS.gene059290:CDS	20.0%
!!!	TTGTTAGTTGTATTGATATT+GGG	-	chr2.2:11475320-11475339	MS.gene059290:CDS	20.0%
!	AAAGATGAAAAGATGTCAAA+TGG	+	chr2.2:11475622-11475641	None:intergenic	25.0%
!	ACTAAATACTATGAGATTCT+TGG	-	chr2.2:11474753-11474772	MS.gene059290:CDS	25.0%
!	ATCATATTGTATTTGTGAAC+AGG	-	chr2.2:11475744-11475763	MS.gene059290:intron	25.0%
!	ATTAAGAATCATCCTGATAA+GGG	-	chr2.2:11474831-11474850	MS.gene059290:CDS	25.0%
!	ATTGGGTTATATAATTGTTG+TGG	-	chr2.2:11475337-11475356	MS.gene059290:intron	25.0%
!	GATTATTGGATTATCTGATT+TGG	-	chr2.2:11474894-11474913	MS.gene059290:CDS	25.0%
!	TTGGTAGAATGATATTTGTT+GGG	-	chr2.2:11475025-11475044	MS.gene059290:intron	25.0%
!!	GGTTTGATTTAATCAATAGT+TGG	+	chr2.2:11476517-11476536	None:intergenic	25.0%
!!	TGCTGAAATTTTCAATCTTT+TGG	-	chr2.2:11476633-11476652	MS.gene059290:intron	25.0%
!!!	GTTTGTTGGTATTTTTCTAA+TGG	-	chr2.2:11475936-11475955	MS.gene059290:intron	25.0%
!!!	TTGGTATTGATTATGCAATT+AGG	-	chr2.2:11475193-11475212	MS.gene059290:CDS	25.0%
	AAAATCAAACTACCATAACC+CGG	+	chr2.2:11475411-11475430	None:intergenic	30.0%
	AATATCAATCCCATTAGCTA+CGG	+	chr2.2:11475141-11475160	None:intergenic	30.0%
	ACACCTAACAACAATCAATT+GGG	+	chr2.2:11475172-11475191	None:intergenic	30.0%
	ATACGCTTTCTTCAAATCAT+CGG	+	chr2.2:11474799-11474818	None:intergenic	30.0%
	ATATTGTATTTGTGAACAGG+TGG	-	chr2.2:11475747-11475766	MS.gene059290:intron	30.0%
	ATGTGAATAATCTACATACC+AGG	+	chr2.2:11476557-11476576	None:intergenic	30.0%
	GATTAAGAATCATCCTGATA+AGG	-	chr2.2:11474830-11474849	MS.gene059290:CDS	30.0%
	GTATGTAGATTATTCACATC+TGG	-	chr2.2:11476558-11476577	MS.gene059290:intron	30.0%
	TACACCTAACAACAATCAAT+TGG	+	chr2.2:11475173-11475192	None:intergenic	30.0%
	TTAGTGTTATCACTCTTCTT+AGG	+	chr2.2:11474738-11474757	None:intergenic	30.0%
	TTTGATTGAATTAGGGTTGT+GGG	-	chr2.2:11474999-11475018	MS.gene059290:CDS	30.0%
!	AAGATATCTATGAGGCATTT+GGG	-	chr2.2:11476054-11476073	MS.gene059290:CDS	30.0%
!	CTATTGATTAAATCAAACCC+TGG	-	chr2.2:11476518-11476537	MS.gene059290:intron	30.0%
!	GACATCTTTTCATCTTTCTT+CGG	-	chr2.2:11475625-11475644	MS.gene059290:CDS	30.0%
!	GTTGGTAGAATGATATTTGT+TGG	-	chr2.2:11475024-11475043	MS.gene059290:intron	30.0%
!	TGGTAGAATGATATTTGTTG+GGG	-	chr2.2:11475026-11475045	MS.gene059290:intron	30.0%
!	TTACCCAATTGATTGTTGTT+AGG	-	chr2.2:11475166-11475185	MS.gene059290:CDS	30.0%
!	TTGATTGTTGTTAGGTGTAT+TGG	-	chr2.2:11475174-11475193	MS.gene059290:CDS	30.0%
!	TTGCATTTGCTGAATCATAT+TGG	+	chr2.2:11476082-11476101	None:intergenic	30.0%
!	TTGGTATTTTTCTAATGGCT+CGG	-	chr2.2:11475941-11475960	MS.gene059290:intron	30.0%
!!	AGGGTTAGTTTGATTGAATT+AGG	-	chr2.2:11474991-11475010	MS.gene059290:CDS	30.0%
!!	GGGTTAGTTTGATTGAATTA+GGG	-	chr2.2:11474992-11475011	MS.gene059290:CDS	30.0%
!!	TTGTTGATTGGTTTGATTAC+AGG	-	chr2.2:11475974-11475993	MS.gene059290:intron	30.0%
	AAATCAAACTACCATAACCC+GGG	+	chr2.2:11475410-11475429	None:intergenic	35.0%
	AACAAACAAGAAACCATACC+AGG	+	chr2.2:11475664-11475683	None:intergenic	35.0%
	ACATCCTTGCAATGAATGTA+AGG	-	chr2.2:11476101-11476120	MS.gene059290:CDS	35.0%
	ACTTGAAGTTCATGTTGAGA+AGG	-	chr2.2:11476200-11476219	MS.gene059290:intron	35.0%
	AGGGTAGTGTGATTGAATTA+AGG	-	chr2.2:11475384-11475403	MS.gene059290:intron	35.0%
	ATATCAATCCCATTAGCTAC+GGG	+	chr2.2:11475140-11475159	None:intergenic	35.0%
	CATCCTTGCAATGAATGTAA+GGG	-	chr2.2:11476102-11476121	MS.gene059290:CDS	35.0%
	CTGGTATGAAGATATCTATG+AGG	-	chr2.2:11476046-11476065	MS.gene059290:CDS	35.0%
	CTTGAAGTTCATGTTGAGAA+GGG	-	chr2.2:11476201-11476220	MS.gene059290:intron	35.0%
	GGAGTTCTTACATTGTTGAT+TGG	-	chr2.2:11475962-11475981	MS.gene059290:intron	35.0%
	GTTTGATTGAATTAGGGTTG+TGG	-	chr2.2:11474998-11475017	MS.gene059290:CDS	35.0%
	TACAAGGCAATCAATGATGA+AGG	-	chr2.2:11476669-11476688	MS.gene059290:intron	35.0%
	TACGCTTTCTTCAAATCATC+GGG	+	chr2.2:11474798-11474817	None:intergenic	35.0%
	TGGTTATGCAGATTCATACA+AGG	-	chr2.2:11476653-11476672	MS.gene059290:intron	35.0%
	TTGAAGAAGAGACAAGAAGA+AGG	-	chr2.2:11476880-11476899	MS.gene059290:CDS	35.0%
	TTGAAGTTCATGTTGAGAAG+GGG	-	chr2.2:11476202-11476221	MS.gene059290:intron	35.0%
	TTGTTGGGGTTTATTTGTCA+TGG	-	chr2.2:11475040-11475059	MS.gene059290:intron	35.0%
!	ATCTTTTCATCTTTCTTCGG+TGG	-	chr2.2:11475628-11475647	MS.gene059290:CDS	35.0%
!	GAAGATATCTATGAGGCATT+TGG	-	chr2.2:11476053-11476072	MS.gene059290:CDS	35.0%
!	GACTGAATTTTGTTCGTCTT+TGG	-	chr2.2:11476610-11476629	MS.gene059290:intron	35.0%
!!	GATTGGTTTGATTACAGGAA+AGG	-	chr2.2:11475979-11475998	MS.gene059290:intron	35.0%
!!	GGGTTATGGTAGTTTGATTT+TGG	-	chr2.2:11475410-11475429	MS.gene059290:intron	35.0%
	AAAGCTTCTTAGATGTCCCA+AGG	+	chr2.2:11475851-11475870	None:intergenic	40.0%
	AAGAATCATCCTGATAAGGG+TGG	-	chr2.2:11474834-11474853	MS.gene059290:CDS	40.0%
	AGGTCTATCTGCATGTTTGT+TGG	-	chr2.2:11475922-11475941	MS.gene059290:intron	40.0%
	AGTGTGATTGAATTAAGGCC+CGG	-	chr2.2:11475389-11475408	MS.gene059290:intron	40.0%
	ATATCTCCAGTAACTGTGTC+CGG	+	chr2.2:11476362-11476381	None:intergenic	40.0%
	ATGTCTGTCAACTGAGATAC+AGG	+	chr2.2:11476816-11476835	None:intergenic	40.0%
	CAACAATCCAGCTCAAACTA+AGG	+	chr2.2:11476298-11476317	None:intergenic	40.0%
	CGTCTTTGTACTTCAACAGA+AGG	-	chr2.2:11476382-11476401	MS.gene059290:intron	40.0%
	CGTGAGGTATATGATCAGTA+TGG	-	chr2.2:11475547-11475566	MS.gene059290:CDS	40.0%
	CTTATTGTTTGTCTTGCAGC+CGG	-	chr2.2:11476340-11476359	MS.gene059290:intron	40.0%
	GCTTTACATTCACTTCACTG+TGG	-	chr2.2:11476728-11476747	MS.gene059290:intron	40.0%
	GTACCCTTACATTCATTGCA+AGG	+	chr2.2:11476108-11476127	None:intergenic	40.0%
	TAAAGCTTGCCCTTCATGAA+AGG	+	chr2.2:11476714-11476733	None:intergenic	40.0%
	TACAGGAAAGGATCAAAGTC+TGG	-	chr2.2:11475991-11476010	MS.gene059290:intron	40.0%
	TATCAATCCCATTAGCTACG+GGG	+	chr2.2:11475139-11475158	None:intergenic	40.0%
	TCACCCTTTCGTTTGAACTT+GGG	+	chr2.2:11476413-11476432	None:intergenic	40.0%
	TGTATCTCAGTTGACAGACA+TGG	-	chr2.2:11476815-11476834	MS.gene059290:intron	40.0%
	TTCACCCTTTCGTTTGAACT+TGG	+	chr2.2:11476414-11476433	None:intergenic	40.0%
	TTGTATTTGTGAACAGGTGG+TGG	-	chr2.2:11475750-11475769	MS.gene059290:intron	40.0%
!	CTTTGGAGGATCTTTACCTT+GGG	-	chr2.2:11475832-11475851	MS.gene059290:CDS	40.0%
!	TACAGTTCCTTAGTTTGAGC+TGG	-	chr2.2:11476288-11476307	MS.gene059290:intron	40.0%
!	TCTTTGGAGGATCTTTACCT+TGG	-	chr2.2:11475831-11475850	MS.gene059290:CDS	40.0%
!	TGCAATGAATGTAAGGGTAC+TGG	-	chr2.2:11476108-11476127	MS.gene059290:CDS	40.0%
!	TTTTCATCTTTCTTCGGTGG+AGG	-	chr2.2:11475631-11475650	MS.gene059290:CDS	40.0%
	AACCACATAAAGCCTCAGTG+AGG	+	chr2.2:11476463-11476482	None:intergenic	45.0%
	AAGGAATGCCAATGTACCAG+AGG	-	chr2.2:11476688-11476707	MS.gene059290:intron	45.0%
	ACACCCCAAGTTCAAACGAA+AGG	-	chr2.2:11476406-11476425	MS.gene059290:intron	45.0%
	ACCACATAAAGCCTCAGTGA+GGG	+	chr2.2:11476462-11476481	None:intergenic	45.0%
	ACCACCCATTCCTTCCTTAA+GGG	+	chr2.2:11475581-11475600	None:intergenic	45.0%
	AGAGCTTGCACAAGCTTATG+AGG	-	chr2.2:11475504-11475523	MS.gene059290:CDS	45.0%
	AGATGCCCTTAAGGAAGGAA+TGG	-	chr2.2:11475573-11475592	MS.gene059290:CDS	45.0%
	ATAGGATCCCCGTAGCTAAT+GGG	-	chr2.2:11475129-11475148	MS.gene059290:intron	45.0%
	ATCCTCCAAAGAGACCTTCA+AGG	+	chr2.2:11475823-11475842	None:intergenic	45.0%
	ATGAAAGGCCTCTGGTACAT+TGG	+	chr2.2:11476699-11476718	None:intergenic	45.0%
	CACCCCAAGTTCAAACGAAA+GGG	-	chr2.2:11476407-11476426	MS.gene059290:intron	45.0%
	CACCCTTTCGTTTGAACTTG+GGG	+	chr2.2:11476412-11476431	None:intergenic	45.0%
	CCAAATGAGTCAGCGCAAAT+TGG	+	chr2.2:11476487-11476506	None:intergenic	45.0%
	GATCAAGAGACAGAGACTCA+GGG	+	chr2.2:11476758-11476777	None:intergenic	45.0%
	GATGAGGATGATGAAATGCC+TGG	-	chr2.2:11476927-11476946	MS.gene059290:CDS	45.0%
	GATGCCCTTAAGGAAGGAAT+GGG	-	chr2.2:11475574-11475593	MS.gene059290:CDS	45.0%
	GTATGGTGAAGATGCCCTTA+AGG	-	chr2.2:11475564-11475583	MS.gene059290:CDS	45.0%
	GTGTGATTGAATTAAGGCCC+GGG	-	chr2.2:11475390-11475409	MS.gene059290:intron	45.0%
	TAACACATACCCCTTGCACT+TGG	+	chr2.2:11475898-11475917	None:intergenic	45.0%
	TAAGGGTGGTGATCCTGAAA+AGG	-	chr2.2:11474848-11474867	MS.gene059290:CDS	45.0%
	TACCAGAGGCCTTTCATGAA+GGG	-	chr2.2:11476702-11476721	MS.gene059290:intron	45.0%
	TATAGGATCCCCGTAGCTAA+TGG	-	chr2.2:11475128-11475147	MS.gene059290:intron	45.0%
	TCATATACCTCACGCTTCTC+AGG	+	chr2.2:11475541-11475560	None:intergenic	45.0%
	TCCTCCAAAGAGACCTTCAA+GGG	+	chr2.2:11475822-11475841	None:intergenic	45.0%
	TCTGTCTCTTGATCAGGTCA+AGG	-	chr2.2:11476764-11476783	MS.gene059290:intron	45.0%
	TCTTGATCAGGTCAAGGCTT+TGG	-	chr2.2:11476770-11476789	MS.gene059290:intron	45.0%
	TGAGTCTCTGTCTCTTGATC+AGG	-	chr2.2:11476758-11476777	MS.gene059290:intron	45.0%
	TGATCAAGAGACAGAGACTC+AGG	+	chr2.2:11476759-11476778	None:intergenic	45.0%
	TTGAATTAAGGCCCGGGTTA+TGG	-	chr2.2:11475396-11475415	MS.gene059290:intron	45.0%
!	AGTGCAAGGGGTATGTGTTA+TGG	-	chr2.2:11475898-11475917	MS.gene059290:CDS	45.0%
!	CAAGGGGTATGTGTTATGGA+AGG	-	chr2.2:11475902-11475921	MS.gene059290:CDS	45.0%
!	GAATTAGGGTTGTGGGTTGT+TGG	-	chr2.2:11475006-11475025	MS.gene059290:CDS	45.0%
!	GCATCGATCTTTGTCACTGA+TGG	+	chr2.2:11476139-11476158	None:intergenic	45.0%
!	TCAGCAAGAGGCATATGATG+AGG	-	chr2.2:11476911-11476930	MS.gene059290:CDS	45.0%
!!	CCAATTTGCGCTGACTCATT+TGG	-	chr2.2:11476484-11476503	MS.gene059290:intron	45.0%
	AACTGAGATACAGGCCTTGC+TGG	+	chr2.2:11476807-11476826	None:intergenic	50.0%
	ACTGAGATACAGGCCTTGCT+GGG	+	chr2.2:11476806-11476825	None:intergenic	50.0%
	AGAACCTTGACAACCAGCAC+AGG	+	chr2.2:11476028-11476047	None:intergenic	50.0%
	CACCACCCATTCCTTCCTTA+AGG	+	chr2.2:11475582-11475601	None:intergenic	50.0%
	CAGGCTTGACAACTTCTCCA+GGG	+	chr2.2:11476538-11476557	None:intergenic	50.0%
	CCTCCAAAGAGACCTTCAAG+GGG	+	chr2.2:11475821-11475840	None:intergenic	50.0%
	CTTAAGGAAGGAATGGGTGG+TGG	-	chr2.2:11475580-11475599	MS.gene059290:CDS	50.0%
	GAGGATGATGAAATGCCTGG+TGG	-	chr2.2:11476930-11476949	MS.gene059290:CDS	50.0%
	GGTCAGAAGATTACCTTCCC+TGG	-	chr2.2:11476234-11476253	MS.gene059290:intron	50.0%
	GGTGAAGATGCCCTTAAGGA+AGG	-	chr2.2:11475568-11475587	MS.gene059290:CDS	50.0%
	GTACCAGAGGCCTTTCATGA+AGG	-	chr2.2:11476701-11476720	MS.gene059290:intron	50.0%
	GTCTTGTGCTCCAAGTGCAA+GGG	-	chr2.2:11475885-11475904	MS.gene059290:CDS	50.0%
	GTGGTAGTAGCAGAGGAAGA+AGG	-	chr2.2:11475769-11475788	MS.gene059290:intron	50.0%
	GTTGAGAAGGGGATGCAGAA+TGG	-	chr2.2:11476213-11476232	MS.gene059290:intron	50.0%
	TCACCCCTTGAAGGTCTCTT+TGG	-	chr2.2:11475815-11475834	MS.gene059290:CDS	50.0%
	TCAGGATCACCACCCTTATC+AGG	+	chr2.2:11474846-11474865	None:intergenic	50.0%
	TCAGTTGACAGACATGGAGC+TGG	-	chr2.2:11476821-11476840	MS.gene059290:intron	50.0%
	TCATCTTTCTTCGGTGGAGG+TGG	-	chr2.2:11475634-11475653	MS.gene059290:CDS	50.0%
	TCTTGTGCTCCAAGTGCAAG+GGG	-	chr2.2:11475886-11475905	MS.gene059290:CDS	50.0%
	TGGAGACAATTCTCCCAGCA+AGG	-	chr2.2:11476790-11476809	MS.gene059290:intron	50.0%
	TGTACTCTTTGAGCACCACC+AGG	+	chr2.2:11476948-11476967	None:intergenic	50.0%
	TGTCTTGTGCTCCAAGTGCA+AGG	-	chr2.2:11475884-11475903	MS.gene059290:CDS	50.0%
	TTCATCAGCTTCACCAGGGA+AGG	+	chr2.2:11476250-11476269	None:intergenic	50.0%
!	TCCCTCACTGAGGCTTTATG+TGG	-	chr2.2:11476458-11476477	MS.gene059290:intron	50.0%
!	TGGAGGTGGATTTCCTGGTA+TGG	-	chr2.2:11475648-11475667	MS.gene059290:CDS	50.0%
!!	ATGACCTGTGCTGGTTGTCA+AGG	-	chr2.2:11476021-11476040	MS.gene059290:intron	50.0%
!!	TGTGCTGGTTGTCAAGGTTC+TGG	-	chr2.2:11476027-11476046	MS.gene059290:intron	50.0%
!!!	GCTGATGAAGCGGTTGGTTT+TGG	-	chr2.2:11476261-11476280	MS.gene059290:intron	50.0%
	AAGAAGGCAGAGACGTGGTG+AGG	-	chr2.2:11475785-11475804	MS.gene059290:intron	55.0%
	AAGGCAGCAAGCTCAGCAAG+AGG	-	chr2.2:11476899-11476918	MS.gene059290:CDS	55.0%
	ACCGCTTCATCAGCTTCACC+AGG	+	chr2.2:11476255-11476274	None:intergenic	55.0%
	ACCTTCTCTCCCTTGCACTG+TGG	+	chr2.2:11476162-11476181	None:intergenic	55.0%
	AGAGGAAGAAGGCAGAGACG+TGG	-	chr2.2:11475780-11475799	MS.gene059290:intron	55.0%
	AGATCGATGCCCACAGTGCA+AGG	-	chr2.2:11476149-11476168	MS.gene059290:CDS	55.0%
	CATGGAGCTGGATGAGTGTG+AGG	-	chr2.2:11476833-11476852	MS.gene059290:intron	55.0%
	CCAGGCTTGACAACTTCTCC+AGG	+	chr2.2:11476539-11476558	None:intergenic	55.0%
	CCCCTTGAAGGTCTCTTTGG+AGG	-	chr2.2:11475818-11475837	MS.gene059290:CDS	55.0%
	CCCTGGTGAAGCTGATGAAG+CGG	-	chr2.2:11476251-11476270	MS.gene059290:intron	55.0%
	CCGCTTCATCAGCTTCACCA+GGG	+	chr2.2:11476254-11476273	None:intergenic	55.0%
	CCTGGAGAAGTTGTCAAGCC+TGG	-	chr2.2:11476536-11476555	MS.gene059290:intron	55.0%
	CCTTCTCTCCCTTGCACTGT+GGG	+	chr2.2:11476161-11476180	None:intergenic	55.0%
	GATCGATGCCCACAGTGCAA+GGG	-	chr2.2:11476150-11476169	MS.gene059290:CDS	55.0%
	GCACACATTGTCCCTCACTG+AGG	-	chr2.2:11476448-11476467	MS.gene059290:intron	55.0%
	GCCCTTAAGGAAGGAATGGG+TGG	-	chr2.2:11475577-11475596	MS.gene059290:CDS	55.0%
	GGATGTCGTTCACCCCTTGA+AGG	-	chr2.2:11475806-11475825	MS.gene059290:CDS	55.0%
	GGTGAAGCTGATGAAGCGGT+TGG	-	chr2.2:11476255-11476274	MS.gene059290:intron	55.0%
	TGCCCTTCATGAAAGGCCTC+TGG	+	chr2.2:11476707-11476726	None:intergenic	55.0%
	TTGCAGCCGGACACAGTTAC+TGG	-	chr2.2:11476353-11476372	MS.gene059290:intron	55.0%
!	AAGGAAGGAATGGGTGGTGG+CGG	-	chr2.2:11475583-11475602	MS.gene059290:CDS	55.0%
!	ACAGGTGGTGGTAGTAGCAG+AGG	-	chr2.2:11475762-11475781	MS.gene059290:intron	55.0%
!	GAGTGATCCTGAGAAGCGTG+AGG	-	chr2.2:11475531-11475550	MS.gene059290:CDS	55.0%
!	TTCGGTGGAGGTGGATTTCC+TGG	-	chr2.2:11475643-11475662	MS.gene059290:CDS	55.0%
	CCCACAGTGCAAGGGAGAGA+AGG	-	chr2.2:11476158-11476177	MS.gene059290:CDS	60.0%
	GGTGCTTCGATGACCTGTGC+TGG	-	chr2.2:11476012-11476031	MS.gene059290:intron	60.0%
!!	GAAGGAATGGGTGGTGGCGG+TGG	-	chr2.2:11475586-11475605	MS.gene059290:CDS	65.0%
!!	GGAATGGGTGGTGGCGGTGG+TGG	-	chr2.2:11475589-11475608	MS.gene059290:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	11474720	11476983	11474720	ID=MS.gene059290
chr2.2	mRNA	11474720	11476983	11474720	ID=MS.gene059290.t1;Parent=MS.gene059290
chr2.2	exon	11476834	11476983	11476834	ID=MS.gene059290.t1.exon1;Parent=MS.gene059290.t1
chr2.2	CDS	11476834	11476983	11476834	ID=cds.MS.gene059290.t1;Parent=MS.gene059290.t1
chr2.2	exon	11476039	11476204	11476039	ID=MS.gene059290.t1.exon2;Parent=MS.gene059290.t1
chr2.2	CDS	11476039	11476204	11476039	ID=cds.MS.gene059290.t1;Parent=MS.gene059290.t1
chr2.2	exon	11475796	11475937	11475796	ID=MS.gene059290.t1.exon3;Parent=MS.gene059290.t1
chr2.2	CDS	11475796	11475937	11475796	ID=cds.MS.gene059290.t1;Parent=MS.gene059290.t1
chr2.2	exon	11475431	11475707	11475431	ID=MS.gene059290.t1.exon4;Parent=MS.gene059290.t1
chr2.2	CDS	11475431	11475707	11475431	ID=cds.MS.gene059290.t1;Parent=MS.gene059290.t1
chr2.2	exon	11475146	11475344	11475146	ID=MS.gene059290.t1.exon5;Parent=MS.gene059290.t1
chr2.2	CDS	11475146	11475344	11475146	ID=cds.MS.gene059290.t1;Parent=MS.gene059290.t1
chr2.2	exon	11474720	11475039	11474720	ID=MS.gene059290.t1.exon6;Parent=MS.gene059290.t1
chr2.2	CDS	11474720	11475039	11474720	ID=cds.MS.gene059290.t1;Parent=MS.gene059290.t1

Gene Sequence

>MS.gene059290

ATGTTTGGAAGAGCGCCTAAGAAGAGTGATAACACTAAATACTATGAGATTCTTGGTGTTTCGAAGAATGCTTCTCCCGATGATTTGAAGAAAGCGTATAAAAAAGCTGCGATTAAGAATCATCCTGATAAGGGTGGTGATCCTGAAAAGGTTGTTTTTTTTATCTTTGAATTTGATTATTGGATTATCTGATTTGGTTTTTGATTGATCAATATATGTTGATGATTGAATTTGATAATTGTTGTGGTTTGAATAGTGATTAGTTGAATTTAGGGTTAGTTTGATTGAATTAGGGTTGTGGGTTGTTGGTAGAATGATATTTGTTGGGGTTTATTTGTCATGGATTATAAATTTGAGATTTTTAAATTAAGGGGAAGCTATTTTACTTTAAAAAAGTGATTTTTTTACTATAGGATCCCCGTAGCTAATGGGATTGATATTGATGATTACCCAATTGATTGTTGTTAGGTGTATTGGTATTGATTATGCAATTAGGATTTTCGTTAATGCTAAAGATTGATAATTGATTGTTAGTTGTAATGATATTGATGATTGAATTATATAATTGTTGTGGTTTGAATTGTGATTAGATAGTTTTGATTGTTAGTTGTATTGATATTGGGTTATATAATTGTTGTGGTTTGAATTGTGATTAGTTAGTTTTAGGGTAGTGTGATTGAATTAAGGCCCGGGTTATGGTAGTTTGATTTTGGTTTTTGTTTTGATTAGTTAATTGATGAAATTAGGGATTAATTTGTGTTTGTTTGTTTTTGATGTAGTTCAAAGAGCTTGCACAAGCTTATGAGGTTTTGAGTGATCCTGAGAAGCGTGAGGTATATGATCAGTATGGTGAAGATGCCCTTAAGGAAGGAATGGGTGGTGGCGGTGGTGGTCACGATCCATTTGACATCTTTTCATCTTTCTTCGGTGGAGGTGGATTTCCTGGTATGGTTTCTTGTTTGTTATTTTAATTTAGGAGATTAGGGATTATTCAATCGATAACTGTTTGTATGATTGTGTCTTAATCATATTGTATTTGTGAACAGGTGGTGGTAGTAGCAGAGGAAGAAGGCAGAGACGTGGTGAGGATGTCGTTCACCCCTTGAAGGTCTCTTTGGAGGATCTTTACCTTGGGACATCTAAGAAGCTTTCTCTTTCACGAAATGTCTTGTGCTCCAAGTGCAAGGGGTATGTGTTATGGAAGGTCTATCTGCATGTTTGTTGGTATTTTTCTAATGGCTCGGAGTTCTTACATTGTTGATTGGTTTGATTACAGGAAAGGATCAAAGTCTGGTGCTTCGATGACCTGTGCTGGTTGTCAAGGTTCTGGTATGAAGATATCTATGAGGCATTTGGGTGCCAATATGATTCAGCAAATGCAACATCCTTGCAATGAATGTAAGGGTACTGGAGAGACCATCAGTGACAAAGATCGATGCCCACAGTGCAAGGGAGAGAAGGTTGTTCAGCAGAAGAAAGTACTTGAAGTTCATGTTGAGAAGGGGATGCAGAATGGTCAGAAGATTACCTTCCCTGGTGAAGCTGATGAAGCGGTTGGTTTTGGATCATACAGTTCCTTAGTTTGAGCTGGATTGTTGAAATTCTAGATACTGATTTTAACTTATTGTTTGTCTTGCAGCCGGACACAGTTACTGGAGATATCGTCTTTGTACTTCAACAGAAGGAACACCCCAAGTTCAAACGAAAGGGTGAAGATCTTTTTGTAGAGCACACATTGTCCCTCACTGAGGCTTTATGTGGTTTCCAATTTGCGCTGACTCATTTGGATTCTCGCCAACTATTGATTAAATCAAACCCTGGAGAAGTTGTCAAGCCTGGTATGTAGATTATTCACATCTGGTTTACTGCATTGTGAAAACATTGTTGAGTGACTGAATTTTGTTCGTCTTTGGTGCTGAAATTTTCAATCTTTTGGTTATGCAGATTCATACAAGGCAATCAATGATGAAGGAATGCCAATGTACCAGAGGCCTTTCATGAAGGGCAAGCTTTACATTCACTTCACTGTGGAGTTCCCTGAGTCTCTGTCTCTTGATCAGGTCAAGGCTTTGGAGACAATTCTCCCAGCAAGGCCTGTATCTCAGTTGACAGACATGGAGCTGGATGAGTGTGAGGAGACAACACTTCATGATGTCAACATTGAAGAAGAGACAAGAAGAAGGCAGCAAGCTCAGCAAGAGGCATATGATGAGGATGATGAAATGCCTGGTGGTGCTCAAAGAGTACAATGTGCTCAGCAGTAA

Protein sequence

>MS.gene059290.t1

MFGRAPKKSDNTKYYEILGVSKNASPDDLKKAYKKAAIKNHPDKGGDPEKFKELAQAYEVLSDPEKREVYDQYGEDALKEGMGGGGGGHDPFDIFSSFFGGGGFPGGGSSRGRRQRRGEDVVHPLKVSLEDLYLGTSKKLSLSRNVLCSKCKGKGSKSGASMTCAGCQGSGMKISMRHLGANMIQQMQHPCNECKGTGETISDKDRCPQCKGEKVVQQKKVLEVHVEKGMQNGQKITFPGEADEAPDTVTGDIVFVLQQKEHPKFKRKGEDLFVEHTLSLTEALCGFQFALTHLDSRQLLIKSNPGEVVKPDSYKAINDEGMPMYQRPFMKGKLYIHFTVEFPESLSLDQVKALETILPARPVSQLTDMELDECEETTLHDVNIEEETRRRQQAQQEAYDEDDEMPGGAQRVQCAQQ