AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56876


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56876.t1 MTR_4g105740 78.967 271 7 1 1 221 1 271 1.44e-151 424
MS.gene56876.t1 MTR_4g105740 78.967 271 7 1 1 221 1 271 2.58e-151 424
MS.gene56876.t1 MTR_3g077870 36.071 280 111 6 13 228 15 290 7.18e-50 166
MS.gene56876.t1 MTR_1g112330 32.331 133 61 3 118 221 398 530 9.00e-15 73.2
MS.gene56876.t1 MTR_5g032190 32.353 136 60 2 118 221 391 526 1.30e-14 72.8
MS.gene56876.t1 MTR_1g112330 31.618 136 61 3 118 221 398 533 2.09e-14 72.0
MS.gene56876.t1 MTR_8g088250 36.082 97 58 2 125 221 161 253 1.10e-13 68.9
MS.gene56876.t1 MTR_1g112330 49.091 55 28 0 118 172 398 452 2.68e-13 68.6
MS.gene56876.t1 MTR_5g029580 28.125 128 68 2 116 219 187 314 1.11e-12 66.6
MS.gene56876.t1 MTR_5g032190 50.000 54 27 0 118 171 391 444 1.42e-12 66.6
MS.gene56876.t1 MTR_5g032190 50.000 54 27 0 118 171 391 444 1.45e-12 66.6
MS.gene56876.t1 MTR_7g011550 32.727 110 53 3 118 206 376 485 6.57e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56876.t1 AT3G47720 30.769 221 84 6 68 228 78 289 2.67e-22 93.2
MS.gene56876.t1 AT5G62520 44.248 113 53 2 119 228 179 284 1.95e-21 90.9
MS.gene56876.t1 AT1G23550 27.660 235 105 5 48 221 56 286 6.59e-21 89.4
MS.gene56876.t1 AT1G70440 24.627 268 129 8 15 221 19 274 7.96e-16 75.1
MS.gene56876.t1 AT1G70440 24.627 268 129 8 15 221 19 274 7.96e-16 75.1

Find 62 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAAACTCATAACATATTC+AGG 0.280626 8.1:-17636904 None:intergenic
ACTCCAAACAATGAACTCTT+TGG 0.300883 8.1:-17636857 None:intergenic
CAAGTACACACTACCTTTGT+TGG 0.301766 8.1:-17636935 None:intergenic
AAACGAATCTGCTTCGGATT+AGG 0.308031 8.1:-17636245 None:intergenic
CCAAGTTTCACAAGGAGTAT+AGG 0.324583 8.1:+17637155 MS.gene56876:CDS
CTCCCAAAGAGTTCATTGTT+TGG 0.353473 8.1:+17636854 MS.gene56876:CDS
AGAGTCGGGAATTGCATAAA+AGG 0.360107 8.1:-17637082 None:intergenic
CAAGTTTCACAAGGAGTATA+GGG 0.369249 8.1:+17637156 MS.gene56876:CDS
CCTATACTCCTTGTGAAACT+TGG 0.373522 8.1:-17637155 None:intergenic
AGTTCAGAAGAATATGATTC+TGG 0.386842 8.1:+17636814 MS.gene56876:CDS
CTCCAAACAATGAACTCTTT+GGG 0.417033 8.1:-17636856 None:intergenic
TAGGGTAAGTGTTTCGGACT+TGG 0.417634 8.1:+17637174 MS.gene56876:CDS
TCGTCTTCCGAACTATCCTT+CGG 0.427547 8.1:-17636203 None:intergenic
TGTTTCGGTAAATTGTAGAT+TGG 0.431614 8.1:-17636111 None:intergenic
TTTGTAGGACATGAGAAGGT+TGG 0.436670 8.1:+17637028 MS.gene56876:intron
AACCACAAACGAATCTGCTT+CGG 0.446185 8.1:-17636251 None:intergenic
TCGCCGGCGGAATCATGTTT+CGG 0.448520 8.1:-17636126 None:intergenic
CGTCTTCCGAACTATCCTTC+GGG 0.448830 8.1:-17636202 None:intergenic
CTGAAAATCGCTACTCCATA+GGG 0.455210 8.1:-17636294 None:intergenic
CCTGAAAATCGCTACTCCAT+AGG 0.467324 8.1:-17636295 None:intergenic
CATGAGAAGGTTGGAGTTGG+AGG 0.468737 8.1:+17637037 MS.gene56876:CDS
TTTCAGGAATCCTTGTAAAA+CGG 0.475306 8.1:+17636311 MS.gene56876:CDS
ATCCGAAGCAGATTCGTTTG+TGG 0.477453 8.1:+17636249 MS.gene56876:CDS
GGACATGAGAAGGTTGGAGT+TGG 0.482722 8.1:+17637034 MS.gene56876:intron
ACGATTCCCGAAGGATAGTT+CGG 0.498656 8.1:+17636196 MS.gene56876:CDS
GGAGTATAGGGTAAGTGTTT+CGG 0.506827 8.1:+17637168 MS.gene56876:CDS
GCGCAAGGGTTGAAACCCTA+TGG 0.518787 8.1:+17636279 MS.gene56876:CDS
GAATCCTTGTAAAACGGTAA+TGG 0.521351 8.1:+17636317 MS.gene56876:CDS
CCTTCGGGAATCGTGGCCGC+CGG 0.528283 8.1:-17636187 None:intergenic
GGATGTGACACTCTCACAAT+CGG 0.535477 8.1:-17636166 None:intergenic
CTCCTTGTGAAACTTGGCAA+TGG 0.542921 8.1:-17637149 None:intergenic
AAAGCGCAGAAATCAGAGTC+GGG 0.547882 8.1:-17637096 None:intergenic
TTCAAACTTGCTTCCAACAA+AGG 0.549408 8.1:+17636922 MS.gene56876:CDS
GTTTGTGGTTTAAGTGCGCA+AGG 0.554303 8.1:+17636264 MS.gene56876:CDS
AATCGCTGATTCATCTTCGC+CGG 0.558062 8.1:-17636142 None:intergenic
GATTGTGAGAGTGTCACATC+CGG 0.562167 8.1:+17636168 MS.gene56876:CDS
AATTGCATAAAAGGCGAAGA+CGG 0.562424 8.1:-17637073 None:intergenic
GCAATGGAAACAATATCAGA+TGG 0.571537 8.1:-17637133 None:intergenic
GAATCATATTCTTCTGAACT+CGG 0.573273 8.1:-17636811 None:intergenic
CGCTGATTCATCTTCGCCGG+CGG 0.587670 8.1:-17636139 None:intergenic
CGGTGGCTAAAATGCGCGGT+GGG 0.591716 8.1:+17636379 MS.gene56876:CDS
TTCCATTGCCAAGTTTCACA+AGG 0.602302 8.1:+17637147 MS.gene56876:CDS
TTTGTGGTTTAAGTGCGCAA+GGG 0.604517 8.1:+17636265 MS.gene56876:CDS
ATGAGAAGGTTGGAGTTGGA+GGG 0.604737 8.1:+17637038 MS.gene56876:CDS
TTACCGAAACATGATTCCGC+CGG 0.606830 8.1:+17636123 MS.gene56876:CDS
TGAGATCTTCGCACGTGCGG+TGG 0.607606 8.1:+17636362 MS.gene56876:CDS
CCGGCGGCCACGATTCCCGA+AGG 0.615886 8.1:+17636187 MS.gene56876:CDS
ATAGTTCGGAAGACGACGGA+GGG 0.618356 8.1:+17636210 MS.gene56876:CDS
CGTGCGGTGGCTAAAATGCG+CGG 0.622389 8.1:+17636375 MS.gene56876:CDS
GAAAGCGCAGAAATCAGAGT+CGG 0.626070 8.1:-17637097 None:intergenic
GAACTATCCTTCGGGAATCG+TGG 0.630182 8.1:-17636194 None:intergenic
GATAGTTCGGAAGACGACGG+AGG 0.630672 8.1:+17636209 MS.gene56876:CDS
AATGTAAAACACGTGTGGTA+CGG 0.641792 8.1:+17636408 MS.gene56876:CDS
GTTTGAGATCTTCGCACGTG+CGG 0.658246 8.1:+17636359 MS.gene56876:CDS
AAACGGTAATGGCACAAGCG+AGG 0.659734 8.1:+17636328 MS.gene56876:CDS
GCGGTGGCTAAAATGCGCGG+TGG 0.662286 8.1:+17636378 MS.gene56876:CDS
AGAAGAATATGATTCTGGAG+TGG 0.669225 8.1:+17636819 MS.gene56876:CDS
ATGGAAACAATATCAGATGG+AGG 0.669944 8.1:-17637130 None:intergenic
ATGCTAATGTAAAACACGTG+TGG 0.673627 8.1:+17636403 MS.gene56876:CDS
TGTGAGAGTGTCACATCCGG+CGG 0.689754 8.1:+17636171 MS.gene56876:CDS
ATTGTTGATATTATTCAACA+CGG 0.694317 8.1:+17636450 MS.gene56876:CDS
AAGGATAGTTCGGAAGACGA+CGG 0.711418 8.1:+17636206 MS.gene56876:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGAATTAACTTTACTTAA+TGG + chr8.1:17636580-17636599 MS.gene56876:intron 15.0%
!!! TTTCAGATTTATTTTTTGTT+TGG + chr8.1:17637004-17637023 MS.gene56876:intron 15.0%
!! ATTAACTTTACTTAATGGTT+AGG + chr8.1:17636585-17636604 MS.gene56876:intron 20.0%
!! ATTGTTGATATTATTCAACA+CGG + chr8.1:17636450-17636469 MS.gene56876:CDS 20.0%
!! TTAACTTTACTTAATGGTTA+GGG + chr8.1:17636586-17636605 MS.gene56876:intron 20.0%
!!! TATTTTTTGTTTGGTTTTGT+AGG + chr8.1:17637013-17637032 MS.gene56876:intron 20.0%
!!! TTGTTGTGTTTTTGTTATTA+GGG + chr8.1:17636975-17636994 MS.gene56876:intron 20.0%
!!! TTTGTTGTGTTTTTGTTATT+AGG + chr8.1:17636974-17636993 MS.gene56876:intron 20.0%
! TTGAAACTCATAACATATTC+AGG - chr8.1:17636907-17636926 None:intergenic 25.0%
AGTTCAGAAGAATATGATTC+TGG + chr8.1:17636814-17636833 MS.gene56876:CDS 30.0%
GAATCATATTCTTCTGAACT+CGG - chr8.1:17636814-17636833 None:intergenic 30.0%
TGTTTCGGTAAATTGTAGAT+TGG - chr8.1:17636114-17636133 None:intergenic 30.0%
TTTCAGGAATCCTTGTAAAA+CGG + chr8.1:17636311-17636330 MS.gene56876:CDS 30.0%
TTTGGTTATGTTCATAGCAA+TGG + chr8.1:17636474-17636493 MS.gene56876:intron 30.0%
! GATATTATTCAACACGGTTT+TGG + chr8.1:17636456-17636475 MS.gene56876:intron 30.0%
AATGTAAAACACGTGTGGTA+CGG + chr8.1:17636408-17636427 MS.gene56876:CDS 35.0%
AATTGCATAAAAGGCGAAGA+CGG - chr8.1:17637076-17637095 None:intergenic 35.0%
ACTCCAAACAATGAACTCTT+TGG - chr8.1:17636860-17636879 None:intergenic 35.0%
ATGCTAATGTAAAACACGTG+TGG + chr8.1:17636403-17636422 MS.gene56876:CDS 35.0%
ATGGAAACAATATCAGATGG+AGG - chr8.1:17637133-17637152 None:intergenic 35.0%
CTCCAAACAATGAACTCTTT+GGG - chr8.1:17636859-17636878 None:intergenic 35.0%
GAATCCTTGTAAAACGGTAA+TGG + chr8.1:17636317-17636336 MS.gene56876:CDS 35.0%
GCAATGGAAACAATATCAGA+TGG - chr8.1:17637136-17637155 None:intergenic 35.0%
! AGAAGAATATGATTCTGGAG+TGG + chr8.1:17636819-17636838 MS.gene56876:CDS 35.0%
! ATGTTGACGAACAAGGTTTT+AGG + chr8.1:17636719-17636738 MS.gene56876:intron 35.0%
! CAAGTTTCACAAGGAGTATA+GGG + chr8.1:17637156-17637175 MS.gene56876:CDS 35.0%
! TTCAAACTTGCTTCCAACAA+AGG + chr8.1:17636922-17636941 MS.gene56876:CDS 35.0%
AAACGAATCTGCTTCGGATT+AGG - chr8.1:17636248-17636267 None:intergenic 40.0%
AACCACAAACGAATCTGCTT+CGG - chr8.1:17636254-17636273 None:intergenic 40.0%
AGAACTGATGTTGACGAACA+AGG + chr8.1:17636712-17636731 MS.gene56876:intron 40.0%
AGAGTCGGGAATTGCATAAA+AGG - chr8.1:17637085-17637104 None:intergenic 40.0%
ATTAACTCGTGCCTTTCAAG+TGG - chr8.1:17636525-17636544 None:intergenic 40.0%
CAAACGATTCTCCACTTGAA+AGG + chr8.1:17636511-17636530 MS.gene56876:intron 40.0%
CAAGTACACACTACCTTTGT+TGG - chr8.1:17636938-17636957 None:intergenic 40.0%
CCTATACTCCTTGTGAAACT+TGG - chr8.1:17637158-17637177 None:intergenic 40.0%
CTCCCAAAGAGTTCATTGTT+TGG + chr8.1:17636854-17636873 MS.gene56876:CDS 40.0%
CTGAAAATCGCTACTCCATA+GGG - chr8.1:17636297-17636316 None:intergenic 40.0%
GGAGTATAGGGTAAGTGTTT+CGG + chr8.1:17637168-17637187 MS.gene56876:CDS 40.0%
TCGTGTGATCATGGGAAAAT+TGG + chr8.1:17636756-17636775 MS.gene56876:intron 40.0%
TGTGATCATGGGAAAATTGG+AGG + chr8.1:17636759-17636778 MS.gene56876:intron 40.0%
TTCCATTGCCAAGTTTCACA+AGG + chr8.1:17637147-17637166 MS.gene56876:CDS 40.0%
TTGCTTTGTCGTGTGATCAT+GGG + chr8.1:17636748-17636767 MS.gene56876:intron 40.0%
TTTGTAGGACATGAGAAGGT+TGG + chr8.1:17637028-17637047 MS.gene56876:intron 40.0%
TTTGTGGTTTAAGTGCGCAA+GGG + chr8.1:17636265-17636284 MS.gene56876:CDS 40.0%
! CCAAGTTTCACAAGGAGTAT+AGG + chr8.1:17637155-17637174 MS.gene56876:CDS 40.0%
! CTTTCAAGTGGAGAATCGTT+TGG - chr8.1:17636513-17636532 None:intergenic 40.0%
! TGTGTACTTGTGTGTTGTTG+TGG + chr8.1:17636948-17636967 MS.gene56876:intron 40.0%
!! TGTGCCATTACCGTTTTACA+AGG - chr8.1:17636324-17636343 None:intergenic 40.0%
!!! TGGTTTTGTAGGACATGAGA+AGG + chr8.1:17637024-17637043 MS.gene56876:intron 40.0%
AAAGCGCAGAAATCAGAGTC+GGG - chr8.1:17637099-17637118 None:intergenic 45.0%
AAGGATAGTTCGGAAGACGA+CGG + chr8.1:17636206-17636225 MS.gene56876:CDS 45.0%
ACGATTCCCGAAGGATAGTT+CGG + chr8.1:17636196-17636215 MS.gene56876:CDS 45.0%
ATCCGAAGCAGATTCGTTTG+TGG + chr8.1:17636249-17636268 MS.gene56876:CDS 45.0%
ATGAGAAGGTTGGAGTTGGA+GGG + chr8.1:17637038-17637057 MS.gene56876:CDS 45.0%
CCTGAAAATCGCTACTCCAT+AGG - chr8.1:17636298-17636317 None:intergenic 45.0%
CTCCTTGTGAAACTTGGCAA+TGG - chr8.1:17637152-17637171 None:intergenic 45.0%
GGATGTGACACTCTCACAAT+CGG - chr8.1:17636169-17636188 None:intergenic 45.0%
GTTTGTGGTTTAAGTGCGCA+AGG + chr8.1:17636264-17636283 MS.gene56876:CDS 45.0%
TAGGGTAAGTGTTTCGGACT+TGG + chr8.1:17637174-17637193 MS.gene56876:CDS 45.0%
TCGTCTTCCGAACTATCCTT+CGG - chr8.1:17636206-17636225 None:intergenic 45.0%
TTACCGAAACATGATTCCGC+CGG + chr8.1:17636123-17636142 MS.gene56876:CDS 45.0%
! CCTATGGAGTAGCGATTTTC+AGG + chr8.1:17636295-17636314 MS.gene56876:CDS 45.0%
! GAAAGCGCAGAAATCAGAGT+CGG - chr8.1:17637100-17637119 None:intergenic 45.0%
! GTTGCTTTGTCGTGTGATCA+TGG + chr8.1:17636747-17636766 MS.gene56876:intron 45.0%
!! AATCGCTGATTCATCTTCGC+CGG - chr8.1:17636145-17636164 None:intergenic 45.0%
!! GATTGTGAGAGTGTCACATC+CGG + chr8.1:17636168-17636187 MS.gene56876:CDS 45.0%
ATAGTTCGGAAGACGACGGA+GGG + chr8.1:17636210-17636229 MS.gene56876:CDS 50.0%
CATGAGAAGGTTGGAGTTGG+AGG + chr8.1:17637037-17637056 MS.gene56876:CDS 50.0%
CGTCTTCCGAACTATCCTTC+GGG - chr8.1:17636205-17636224 None:intergenic 50.0%
GAACTATCCTTCGGGAATCG+TGG - chr8.1:17636197-17636216 None:intergenic 50.0%
GGACATGAGAAGGTTGGAGT+TGG + chr8.1:17637034-17637053 MS.gene56876:intron 50.0%
! AAACGGTAATGGCACAAGCG+AGG + chr8.1:17636328-17636347 MS.gene56876:CDS 50.0%
! GTTTGAGATCTTCGCACGTG+CGG + chr8.1:17636359-17636378 MS.gene56876:CDS 50.0%
AAATTGGAGGCTGTGCCTGC+AGG + chr8.1:17636772-17636791 MS.gene56876:intron 55.0%
GATAGTTCGGAAGACGACGG+AGG + chr8.1:17636209-17636228 MS.gene56876:CDS 55.0%
GCGCAAGGGTTGAAACCCTA+TGG + chr8.1:17636279-17636298 MS.gene56876:CDS 55.0%
TCGCCGGCGGAATCATGTTT+CGG - chr8.1:17636129-17636148 None:intergenic 55.0%
! CTGCGTTGATCAGAACCTGC+AGG - chr8.1:17636790-17636809 None:intergenic 55.0%
!! TGTGAGAGTGTCACATCCGG+CGG + chr8.1:17636171-17636190 MS.gene56876:CDS 55.0%
CGGTGGCTAAAATGCGCGGT+GGG + chr8.1:17636379-17636398 MS.gene56876:CDS 60.0%
CGTGCGGTGGCTAAAATGCG+CGG + chr8.1:17636375-17636394 MS.gene56876:CDS 60.0%
TGAGATCTTCGCACGTGCGG+TGG + chr8.1:17636362-17636381 MS.gene56876:CDS 60.0%
!! CGCTGATTCATCTTCGCCGG+CGG - chr8.1:17636142-17636161 None:intergenic 60.0%
GCGGTGGCTAAAATGCGCGG+TGG + chr8.1:17636378-17636397 MS.gene56876:CDS 65.0%
CCTTCGGGAATCGTGGCCGC+CGG - chr8.1:17636190-17636209 None:intergenic 70.0%
CCGGCGGCCACGATTCCCGA+AGG + chr8.1:17636187-17636206 MS.gene56876:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 17636102 17637201 17636102 ID=MS.gene56876
chr8.1 mRNA 17636102 17637201 17636102 ID=MS.gene56876.t1;Parent=MS.gene56876
chr8.1 exon 17636102 17636471 17636102 ID=MS.gene56876.t1.exon1;Parent=MS.gene56876.t1
chr8.1 CDS 17636102 17636471 17636102 ID=cds.MS.gene56876.t1;Parent=MS.gene56876.t1
chr8.1 exon 17636794 17636943 17636794 ID=MS.gene56876.t1.exon2;Parent=MS.gene56876.t1
chr8.1 CDS 17636794 17636943 17636794 ID=cds.MS.gene56876.t1;Parent=MS.gene56876.t1
chr8.1 exon 17637035 17637201 17637035 ID=MS.gene56876.t1.exon3;Parent=MS.gene56876.t1
chr8.1 CDS 17637035 17637201 17637035 ID=cds.MS.gene56876.t1;Parent=MS.gene56876.t1
Gene Sequence

>MS.gene56876

ATGGAACAACCAATCTACAATTTACCGAAACATGATTCCGCCGGCGAAGATGAATCAGCGATTTCCGATTGTGAGAGTGTCACATCCGGCGGCCACGATTCCCGAAGGATAGTTCGGAAGACGACGGAGGGTGCAGATATCAACCTAATCCGAAGCAGATTCGTTTGTGGTTTAAGTGCGCAAGGGTTGAAACCCTATGGAGTAGCGATTTTCAGGAATCCTTGTAAAACGGTAATGGCACAAGCGAGGATTCAATCGTTTGAGATCTTCGCACGTGCGGTGGCTAAAATGCGCGGTGGGAATGCTAATGTAAAACACGTGTGGTACGGTGCTTCGAGTAGAGAAGAGATTGTTGATATTATTCAACACGGTTTTGGTTATGTTCATAGCAATGGATTGCGTCTTTCTCCAAACGATTCTCCACTTGAAAGGCACGAGTTAATTTCATTATTATTTAATTGATTAGTTTAGTTAGTTAATTGAATTAACTTTACTTAATGGTTAGGGTTTAAAAATTAATTAATTTAATTGATGTAGATTTTGTGATTTTGATTTGTGATGTAATGCGTATTTTTTTTTATTTTTTTTTTACTGTTTTTACAGTGTGAAAAGAACTGATGTTGACGAACAAGGTTTTAGGCACTTGTTGCTTTGTCGTGTGATCATGGGAAAATTGGAGGCTGTGCCTGCAGGTTCTGATCAACGCAGACCGAGTTCAGAAGAATATGATTCTGGAGTGGATAGTTTTTCATCTCCCAAAGAGTTCATTGTTTGGAGTAACAAGATCAACACGCATGTCTTGCCTGAATATGTTATGAGTTTCAAACTTGCTTCCAACAAAGGTAGTGTGTACTTGTGTGTTGTTGTGGTTATTTGTTGTGTTTTTGTTATTAGGGATGATGTTTCAGATTTATTTTTTGTTTGGTTTTGTAGGACATGAGAAGGTTGGAGTTGGAGGGCAACATGTGAGACCGTCTTCGCCTTTTATGCAATTCCCGACTCTGATTTCTGCGCTTTCGAAGATATTGCCTCCATCTGATATTGTTTCCATTGCCAAGTTTCACAAGGAGTATAGGGTAAGTGTTTCGGACTTGGTTTGA

Protein sequence

>MS.gene56876.t1

MEQPIYNLPKHDSAGEDESAISDCESVTSGGHDSRRIVRKTTEGADINLIRSRFVCGLSAQGLKPYGVAIFRNPCKTVMAQARIQSFEIFARAVAKMRGGNANVKHVWYGASSREEIVDIIQHGSDQRRPSSEEYDSGVDSFSSPKEFIVWSNKINTHVLPEYVMSFKLASNKGHEKVGVGGQHVRPSSPFMQFPTLISALSKILPPSDIVSIAKFHKEYRVSVSDLV