Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56931.t1 | XP_003608942.1 | 96.1 | 178 | 7 | 0 | 1 | 178 | 1 | 178 | 8.60E-84 | 319.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56931.t1 | Q9FYL8 | 56.4 | 149 | 65 | 0 | 30 | 178 | 42 | 190 | 2.5e-39 | 163.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56931.t1 | G7JNT6 | 96.1 | 178 | 7 | 0 | 1 | 178 | 1 | 178 | 6.2e-84 | 319.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene56931 | -0.806533 | 7.86E-50 | -1.69E-46 |
| MS.gene050114 | MS.gene56931 | -0.824262 | 8.88E-54 | -1.69E-46 |
| MS.gene056975 | MS.gene56931 | -0.821162 | 4.67E-53 | -1.69E-46 |
| MS.gene057451 | MS.gene56931 | 0.818206 | 2.21E-52 | -1.69E-46 |
| MS.gene057452 | MS.gene56931 | 0.853335 | 2.56E-61 | -1.69E-46 |
| MS.gene059872 | MS.gene56931 | 0.818466 | 1.93E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56931.t1 | MTR_4g106640 | 96.067 | 178 | 7 | 0 | 1 | 178 | 1 | 178 | 4.26e-122 | 342 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56931.t1 | AT1G24450 | 56.376 | 149 | 65 | 0 | 30 | 178 | 42 | 190 | 9.77e-56 | 174 |
Find 39 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGCAAACCCTAGTTGTTTC+TGG | 0.221287 | 8.1:-18702651 | None:intergenic |
| ACTGTTCATGGTCGTGATCT+TGG | 0.284490 | 8.1:+18703151 | MS.gene56931:CDS |
| CTGTTCATGGTCGTGATCTT+GGG | 0.329301 | 8.1:+18703152 | MS.gene56931:CDS |
| CAAGCGACGGTTCAACTCTT+TGG | 0.330495 | 8.1:-18702939 | None:intergenic |
| TGCCGTTGATGCAATGCGTT+TGG | 0.351005 | 8.1:+18702991 | MS.gene56931:CDS |
| TCCACATGCTACCATCGTTC+AGG | 0.351797 | 8.1:+18702488 | MS.gene56931:CDS |
| ATGAGTCGACATTGGAGATT+AGG | 0.358387 | 8.1:-18702964 | None:intergenic |
| TCTTCCACTGCTGTGGTTTG+TGG | 0.372837 | 8.1:+18703055 | MS.gene56931:CDS |
| TTCTCTTGATACTCGTAGTT+CGG | 0.377762 | 8.1:+18703105 | MS.gene56931:CDS |
| CACAGCAGTGGAAGAAGAAT+TGG | 0.384003 | 8.1:-18703047 | None:intergenic |
| GTTTCGAGTGCGGATGAAAA+TGG | 0.387367 | 8.1:-18702625 | None:intergenic |
| GAAAGCTTTGTTGTTCTCTT+CGG | 0.417815 | 8.1:-18702843 | None:intergenic |
| TGAGTCGACATTGGAGATTA+GGG | 0.444608 | 8.1:-18702963 | None:intergenic |
| CAATTCTTCTTCCACTGCTG+TGG | 0.459397 | 8.1:+18703048 | MS.gene56931:CDS |
| GCCGTTGATGCAATGCGTTT+GGG | 0.462426 | 8.1:+18702992 | MS.gene56931:CDS |
| AAGGATCGCAATGAGTAAAA+TGG | 0.466869 | 8.1:-18702466 | None:intergenic |
| ACCCAAACGCATTGCATCAA+CGG | 0.482026 | 8.1:-18702993 | None:intergenic |
| AGTGGAAGAAGAATTGGTCT+TGG | 0.487457 | 8.1:-18703041 | None:intergenic |
| GTGGAAGAAGAATTGGTCTT+GGG | 0.501015 | 8.1:-18703040 | None:intergenic |
| TACTCGTAGTTCGGAGTCTG+CGG | 0.502718 | 8.1:+18703114 | MS.gene56931:CDS |
| CAAACCCTAGTTGTTTCTGG+AGG | 0.510081 | 8.1:-18702648 | None:intergenic |
| GAAACCCTCCAGAAACAACT+AGG | 0.510438 | 8.1:+18702643 | MS.gene56931:CDS |
| ACTCGTAGTTCGGAGTCTGC+GGG | 0.537136 | 8.1:+18703115 | MS.gene56931:CDS |
| AAACCCTAGTTGTTTCTGGA+GGG | 0.539563 | 8.1:-18702647 | None:intergenic |
| CTCTTCGGAGAAAGAAGCGT+GGG | 0.560654 | 8.1:-18702828 | None:intergenic |
| TTTCTGGAGGGTTTCGAGTG+CGG | 0.561747 | 8.1:-18702635 | None:intergenic |
| GCGACGGTTCAACTCTTTGG+CGG | 0.570405 | 8.1:-18702936 | None:intergenic |
| TGTTCATGGTCGTGATCTTG+GGG | 0.587592 | 8.1:+18703153 | MS.gene56931:CDS |
| GGCACAAGATGAGTCGACAT+TGG | 0.593448 | 8.1:-18702972 | None:intergenic |
| AGCACCACAAACCACAGCAG+TGG | 0.604051 | 8.1:-18703059 | None:intergenic |
| GAACGATGGTAGCATGTGGA+AGG | 0.616582 | 8.1:-18702485 | None:intergenic |
| TAAAATGGTGAAGACGATGA+AGG | 0.618114 | 8.1:-18702451 | None:intergenic |
| GCGTTTGGGTTTGCACAAGG+TGG | 0.620407 | 8.1:+18703006 | MS.gene56931:CDS |
| AATGCGTTTGGGTTTGCACA+AGG | 0.636231 | 8.1:+18703003 | MS.gene56931:CDS |
| ACCTGAACGATGGTAGCATG+TGG | 0.640546 | 8.1:-18702489 | None:intergenic |
| AAACCCTCCAGAAACAACTA+GGG | 0.642154 | 8.1:+18702644 | MS.gene56931:CDS |
| TGGAGATTAGGGACAAGCGA+CGG | 0.678870 | 8.1:-18702952 | None:intergenic |
| TCTCTTCGGAGAAAGAAGCG+TGG | 0.707050 | 8.1:-18702829 | None:intergenic |
| GGAAAAGGAAACCTGAACGA+TGG | 0.708272 | 8.1:-18702499 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTTTTTGACCAAATT+CGG | + | chr8.1:18702725-18702744 | MS.gene56931:intron | 15.0% |
| !! | ATAGAATAAAATGAAAAGAG+AGG | - | chr8.1:18702697-18702716 | None:intergenic | 20.0% |
| !! | TAGAATAAAATGAAAAGAGA+GGG | - | chr8.1:18702696-18702715 | None:intergenic | 20.0% |
| !! | TTAACAAATTAAACCGAATT+TGG | - | chr8.1:18702741-18702760 | None:intergenic | 20.0% |
| ! | AAAAGCAAAAGATAGAGTAA+TGG | - | chr8.1:18702580-18702599 | None:intergenic | 25.0% |
| !!! | AATGGAAACTGTTTCTATTA+AGG | - | chr8.1:18702562-18702581 | None:intergenic | 25.0% |
| !!! | ATGGAAACTGTTTCTATTAA+GGG | - | chr8.1:18702561-18702580 | None:intergenic | 25.0% |
| ATGTGTATTACATGATGATC+AGG | + | chr8.1:18702765-18702784 | MS.gene56931:intron | 30.0% | |
| GTTTCTATTAAGGGATTGTT+CGG | - | chr8.1:18702552-18702571 | None:intergenic | 30.0% | |
| ! | AACAAAGCTTTCAGCATTTT+AGG | + | chr8.1:18702854-18702873 | MS.gene56931:CDS | 30.0% |
| ! | AATGTTTTCTTGACTGTTCA+TGG | + | chr8.1:18703139-18703158 | MS.gene56931:CDS | 30.0% |
| AAGGATCGCAATGAGTAAAA+TGG | - | chr8.1:18702469-18702488 | None:intergenic | 35.0% | |
| AGAGAGGGTTTCTGAATAAA+AGG | - | chr8.1:18702681-18702700 | None:intergenic | 35.0% | |
| GAGAAAAATGAGAGAGAAAG+GGG | - | chr8.1:18702523-18702542 | None:intergenic | 35.0% | |
| GGAGAAAAATGAGAGAGAAA+GGG | - | chr8.1:18702524-18702543 | None:intergenic | 35.0% | |
| GGTTTCTGAATAAAAGGACA+GGG | - | chr8.1:18702675-18702694 | None:intergenic | 35.0% | |
| GTTTCTGAATAAAAGGACAG+GGG | - | chr8.1:18702674-18702693 | None:intergenic | 35.0% | |
| TAAAATGGTGAAGACGATGA+AGG | - | chr8.1:18702454-18702473 | None:intergenic | 35.0% | |
| TGGAGAAAAATGAGAGAGAA+AGG | - | chr8.1:18702525-18702544 | None:intergenic | 35.0% | |
| TTCTCTTGATACTCGTAGTT+CGG | + | chr8.1:18703105-18703124 | MS.gene56931:CDS | 35.0% | |
| ! | GGTGCTTTTCGATCAATTTT+TGG | + | chr8.1:18703076-18703095 | MS.gene56931:CDS | 35.0% |
| ! | TTAAGGGATTGTTCGGATTT+TGG | - | chr8.1:18702545-18702564 | None:intergenic | 35.0% |
| !! | GAAAGCTTTGTTGTTCTCTT+CGG | - | chr8.1:18702846-18702865 | None:intergenic | 35.0% |
| AAACCCTCCAGAAACAACTA+GGG | + | chr8.1:18702644-18702663 | MS.gene56931:CDS | 40.0% | |
| AATGAGAGAGAAAGGGGAAA+AGG | - | chr8.1:18702517-18702536 | None:intergenic | 40.0% | |
| ATGAGTCGACATTGGAGATT+AGG | - | chr8.1:18702967-18702986 | None:intergenic | 40.0% | |
| GGGTTTCTGAATAAAAGGAC+AGG | - | chr8.1:18702676-18702695 | None:intergenic | 40.0% | |
| TGAGTCGACATTGGAGATTA+GGG | - | chr8.1:18702966-18702985 | None:intergenic | 40.0% | |
| ! | AGTGGAAGAAGAATTGGTCT+TGG | - | chr8.1:18703044-18703063 | None:intergenic | 40.0% |
| !! | AAACCCTAGTTGTTTCTGGA+GGG | - | chr8.1:18702650-18702669 | None:intergenic | 40.0% |
| !! | GTGGAAGAAGAATTGGTCTT+GGG | - | chr8.1:18703043-18703062 | None:intergenic | 40.0% |
| ACCCAAACGCATTGCATCAA+CGG | - | chr8.1:18702996-18703015 | None:intergenic | 45.0% | |
| ACTGTTCATGGTCGTGATCT+TGG | + | chr8.1:18703151-18703170 | MS.gene56931:CDS | 45.0% | |
| CAATTCTTCTTCCACTGCTG+TGG | + | chr8.1:18703048-18703067 | MS.gene56931:CDS | 45.0% | |
| CACAGCAGTGGAAGAAGAAT+TGG | - | chr8.1:18703050-18703069 | None:intergenic | 45.0% | |
| CTGTTCATGGTCGTGATCTT+GGG | + | chr8.1:18703152-18703171 | MS.gene56931:CDS | 45.0% | |
| GAAACCCTCCAGAAACAACT+AGG | + | chr8.1:18702643-18702662 | MS.gene56931:CDS | 45.0% | |
| GGAAAAGGAAACCTGAACGA+TGG | - | chr8.1:18702502-18702521 | None:intergenic | 45.0% | |
| TGTTCATGGTCGTGATCTTG+GGG | + | chr8.1:18703153-18703172 | MS.gene56931:CDS | 45.0% | |
| ! | AATGCGTTTGGGTTTGCACA+AGG | + | chr8.1:18703003-18703022 | MS.gene56931:CDS | 45.0% |
| ! | GTTTCGAGTGCGGATGAAAA+TGG | - | chr8.1:18702628-18702647 | None:intergenic | 45.0% |
| !! | CAAACCCTAGTTGTTTCTGG+AGG | - | chr8.1:18702651-18702670 | None:intergenic | 45.0% |
| !! | CTTTTGCTTTTGTGCCTGCA+GGG | + | chr8.1:18702589-18702608 | MS.gene56931:intron | 45.0% |
| !! | TCTTTTGCTTTTGTGCCTGC+AGG | + | chr8.1:18702588-18702607 | MS.gene56931:intron | 45.0% |
| ACCTGAACGATGGTAGCATG+TGG | - | chr8.1:18702492-18702511 | None:intergenic | 50.0% | |
| CAAGCGACGGTTCAACTCTT+TGG | - | chr8.1:18702942-18702961 | None:intergenic | 50.0% | |
| GAACGATGGTAGCATGTGGA+AGG | - | chr8.1:18702488-18702507 | None:intergenic | 50.0% | |
| GCCGTTGATGCAATGCGTTT+GGG | + | chr8.1:18702992-18703011 | MS.gene56931:CDS | 50.0% | |
| GGCACAAGATGAGTCGACAT+TGG | - | chr8.1:18702975-18702994 | None:intergenic | 50.0% | |
| TACTCGTAGTTCGGAGTCTG+CGG | + | chr8.1:18703114-18703133 | MS.gene56931:CDS | 50.0% | |
| TCCACATGCTACCATCGTTC+AGG | + | chr8.1:18702488-18702507 | MS.gene56931:CDS | 50.0% | |
| TCTCTTCGGAGAAAGAAGCG+TGG | - | chr8.1:18702832-18702851 | None:intergenic | 50.0% | |
| TCTTCCACTGCTGTGGTTTG+TGG | + | chr8.1:18703055-18703074 | MS.gene56931:CDS | 50.0% | |
| TGCCGTTGATGCAATGCGTT+TGG | + | chr8.1:18702991-18703010 | MS.gene56931:CDS | 50.0% | |
| TGGAGATTAGGGACAAGCGA+CGG | - | chr8.1:18702955-18702974 | None:intergenic | 50.0% | |
| ! | CTCTTCGGAGAAAGAAGCGT+GGG | - | chr8.1:18702831-18702850 | None:intergenic | 50.0% |
| ! | GGGCAAACCCTAGTTGTTTC+TGG | - | chr8.1:18702654-18702673 | None:intergenic | 50.0% |
| ! | TTTCTGGAGGGTTTCGAGTG+CGG | - | chr8.1:18702638-18702657 | None:intergenic | 50.0% |
| ACTCGTAGTTCGGAGTCTGC+GGG | + | chr8.1:18703115-18703134 | MS.gene56931:CDS | 55.0% | |
| AGCACCACAAACCACAGCAG+TGG | - | chr8.1:18703062-18703081 | None:intergenic | 55.0% | |
| GCGACGGTTCAACTCTTTGG+CGG | - | chr8.1:18702939-18702958 | None:intergenic | 55.0% | |
| GTGAGAATGAGTGACCCTGC+AGG | - | chr8.1:18702606-18702625 | None:intergenic | 55.0% | |
| ! | GCGTTTGGGTTTGCACAAGG+TGG | + | chr8.1:18703006-18703025 | MS.gene56931:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 18702435 | 18703192 | 18702435 | ID=MS.gene56931 |
| chr8.1 | mRNA | 18702435 | 18703192 | 18702435 | ID=MS.gene56931.t1;Parent=MS.gene56931 |
| chr8.1 | exon | 18702435 | 18702509 | 18702435 | ID=MS.gene56931.t1.exon1;Parent=MS.gene56931.t1 |
| chr8.1 | CDS | 18702435 | 18702509 | 18702435 | ID=cds.MS.gene56931.t1;Parent=MS.gene56931.t1 |
| chr8.1 | exon | 18702610 | 18702665 | 18702610 | ID=MS.gene56931.t1.exon2;Parent=MS.gene56931.t1 |
| chr8.1 | CDS | 18702610 | 18702665 | 18702610 | ID=cds.MS.gene56931.t1;Parent=MS.gene56931.t1 |
| chr8.1 | exon | 18702787 | 18703192 | 18702787 | ID=MS.gene56931.t1.exon3;Parent=MS.gene56931.t1 |
| chr8.1 | CDS | 18702787 | 18703192 | 18702787 | ID=cds.MS.gene56931.t1;Parent=MS.gene56931.t1 |
| Gene Sequence |
| Protein sequence |
