Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56948.t1 | XP_003608955.1 | 86.6 | 187 | 19 | 1 | 1 | 181 | 1 | 187 | 1.00E-76 | 296.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56948.t1 | G7JNU9 | 86.6 | 187 | 19 | 1 | 1 | 181 | 1 | 187 | 7.4e-77 | 296.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene074378 | MS.gene56948 | PPI |
| MS.gene25931 | MS.gene56948 | PPI |
| MS.gene022274 | MS.gene56948 | PPI |
| MS.gene70603 | MS.gene56948 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56948.t1 | MTR_4g106790 | 94.652 | 187 | 4 | 1 | 1 | 181 | 1 | 187 | 1.17e-128 | 359 |
| MS.gene56948.t1 | MTR_4g106790 | 93.243 | 148 | 4 | 1 | 1 | 142 | 1 | 148 | 3.03e-98 | 281 |
| MS.gene56948.t1 | MTR_3g078300 | 54.011 | 187 | 76 | 3 | 1 | 181 | 1 | 183 | 5.25e-60 | 185 |
| MS.gene56948.t1 | MTR_3g078300 | 54.054 | 148 | 58 | 3 | 1 | 142 | 1 | 144 | 3.01e-45 | 147 |
| MS.gene56948.t1 | MTR_5g035430 | 43.956 | 182 | 87 | 4 | 1 | 179 | 1 | 170 | 4.88e-40 | 134 |
| MS.gene56948.t1 | MTR_4g097220 | 39.011 | 182 | 96 | 4 | 1 | 181 | 1 | 168 | 2.16e-34 | 119 |
| MS.gene56948.t1 | MTR_8g063200 | 39.888 | 178 | 99 | 4 | 1 | 178 | 1 | 170 | 1.77e-30 | 109 |
| MS.gene56948.t1 | MTR_4g097220 | 43.307 | 127 | 59 | 2 | 1 | 127 | 1 | 114 | 6.03e-29 | 104 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56948.t1 | AT5G62900 | 47.514 | 181 | 75 | 4 | 1 | 181 | 1 | 161 | 1.42e-47 | 152 |
| MS.gene56948.t1 | AT5G50090 | 47.253 | 182 | 71 | 4 | 1 | 181 | 1 | 158 | 4.42e-42 | 139 |
| MS.gene56948.t1 | AT5G50090 | 48.066 | 181 | 65 | 5 | 1 | 181 | 1 | 152 | 1.89e-41 | 137 |
| MS.gene56948.t1 | AT1G60010 | 42.553 | 188 | 86 | 5 | 1 | 181 | 1 | 173 | 6.08e-41 | 136 |
| MS.gene56948.t1 | AT5G50090 | 58.407 | 113 | 40 | 1 | 1 | 113 | 1 | 106 | 8.01e-36 | 122 |
| MS.gene56948.t1 | AT1G10530 | 37.297 | 185 | 93 | 4 | 1 | 181 | 1 | 166 | 3.68e-33 | 116 |
| MS.gene56948.t1 | AT5G67620 | 32.323 | 198 | 100 | 7 | 1 | 181 | 1 | 181 | 1.53e-23 | 92.0 |
Find 27 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCTTTCTTCTTTGCCATT+TGG | 0.302263 | 8.1:-18954482 | None:intergenic |
| ACAAAAGTCAAATCAGATTA+AGG | 0.303462 | 8.1:+18954884 | MS.gene56948:CDS |
| AGATTTACCTTGTTATCTTC+AGG | 0.363890 | 8.1:-18954942 | None:intergenic |
| TTAATGATAAAACAGCTAAT+AGG | 0.376595 | 8.1:+18954913 | MS.gene56948:CDS |
| ATCACTTCACTAGCAGTAAC+TGG | 0.406024 | 8.1:-18954512 | None:intergenic |
| AGAAGAGCAACGTAGTGATC+AGG | 0.425975 | 8.1:-18954545 | None:intergenic |
| CAGAGGTTACAAAGGGTATG+TGG | 0.456771 | 8.1:+18954814 | MS.gene56948:intron |
| TTCAGTGATGCTTTGTAAAG+AGG | 0.477664 | 8.1:-18955525 | None:intergenic |
| GCAAAGAAGAAAGGTTGTAT+TGG | 0.478560 | 8.1:+18954489 | MS.gene56948:CDS |
| AGGACAAAAGCAACAACAAA+TGG | 0.492369 | 8.1:+18955467 | MS.gene56948:CDS |
| AACCAAATGGCAAAGAAGAA+AGG | 0.503021 | 8.1:+18954480 | MS.gene56948:CDS |
| TCAGTGATGCTTTGTAAAGA+GGG | 0.514695 | 8.1:-18955524 | None:intergenic |
| CCAACAGATACACTCTTGCT+TGG | 0.516609 | 8.1:+18954668 | MS.gene56948:CDS |
| CTTGTAATACAACAACCAAA+TGG | 0.516825 | 8.1:+18954467 | MS.gene56948:CDS |
| TTGGTTTCAGAGGTTACAAA+GGG | 0.536135 | 8.1:+18954807 | MS.gene56948:intron |
| GATTCAAAACTCATATCAGC+TGG | 0.538326 | 8.1:-18955552 | None:intergenic |
| TATAGACTCATCTCAGCTCA+AGG | 0.571396 | 8.1:+18954698 | MS.gene56948:CDS |
| GAAGAGCAACGTAGTGATCA+GGG | 0.575596 | 8.1:-18954544 | None:intergenic |
| AGAGGTTACAAAGGGTATGT+GGG | 0.580561 | 8.1:+18954815 | MS.gene56948:intron |
| TTTGGTTTCAGAGGTTACAA+AGG | 0.584060 | 8.1:+18954806 | MS.gene56948:intron |
| TTTGGTTGTTGTATTACAAG+AGG | 0.585445 | 8.1:-18954464 | None:intergenic |
| GTCTGAACCTGAAGATAACA+AGG | 0.608807 | 8.1:+18954935 | MS.gene56948:CDS |
| AGTCAAATCAGATTAAGGAA+AGG | 0.621653 | 8.1:+18954889 | MS.gene56948:CDS |
| CCAAGCAAGAGTGTATCTGT+TGG | 0.622428 | 8.1:-18954668 | None:intergenic |
| AATCTGAAAGACATGAGTCA+AGG | 0.637514 | 8.1:+18955447 | MS.gene56948:CDS |
| ACAAAAGCAACAACAAATGG+TGG | 0.646145 | 8.1:+18955470 | MS.gene56948:CDS |
| ATAATAATAAGTCAAGAACA+TGG | 0.691937 | 8.1:+18955498 | MS.gene56948:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACTAATAATAATTTATGAT+TGG | + | chr8.1:18955403-18955422 | MS.gene56948:intron | 10.0% |
| !! | TTATTTACATTAACTAATTA+AGG | + | chr8.1:18955256-18955275 | MS.gene56948:intron | 10.0% |
| !! | AAAACAAAATAATCTCTTTA+AGG | - | chr8.1:18954761-18954780 | None:intergenic | 15.0% |
| !! | AAACAAAATAATCTCTTTAA+GGG | - | chr8.1:18954760-18954779 | None:intergenic | 15.0% |
| !!! | TTTATTTATTCTTTTGTTTG+TGG | + | chr8.1:18955158-18955177 | MS.gene56948:intron | 15.0% |
| !! | ATAATAATAAGTCAAGAACA+TGG | + | chr8.1:18955498-18955517 | MS.gene56948:CDS | 20.0% |
| !! | TATAATTACTGTTCATAAGA+AGG | - | chr8.1:18955090-18955109 | None:intergenic | 20.0% |
| !! | TTAATGATAAAACAGCTAAT+AGG | + | chr8.1:18954913-18954932 | MS.gene56948:CDS | 20.0% |
| ! | AAGAACATAAATATCTTGAG+TGG | + | chr8.1:18955023-18955042 | MS.gene56948:intron | 25.0% |
| ! | ACAAAAGTCAAATCAGATTA+AGG | + | chr8.1:18954884-18954903 | MS.gene56948:CDS | 25.0% |
| ! | CATAATATAAGCAAAGTTTG+AGG | - | chr8.1:18955190-18955209 | None:intergenic | 25.0% |
| ! | TGCCAATAAAATTTATAACC+AGG | - | chr8.1:18955282-18955301 | None:intergenic | 25.0% |
| !!! | ATGATTGGTTCATTATTTTC+AGG | + | chr8.1:18955418-18955437 | MS.gene56948:intron | 25.0% |
| !!! | TTGAGTCTTTATGTTGTTTT+TGG | + | chr8.1:18954788-18954807 | MS.gene56948:intron | 25.0% |
| AGATTCAATCAACATGGATT+TGG | - | chr8.1:18955001-18955020 | None:intergenic | 30.0% | |
| AGTCAAATCAGATTAAGGAA+AGG | + | chr8.1:18954889-18954908 | MS.gene56948:CDS | 30.0% | |
| CTTGTAATACAACAACCAAA+TGG | + | chr8.1:18954467-18954486 | MS.gene56948:CDS | 30.0% | |
| TACATTAACTAATTAAGGCC+TGG | + | chr8.1:18955261-18955280 | MS.gene56948:intron | 30.0% | |
| TTTGGTTGTTGTATTACAAG+AGG | - | chr8.1:18954467-18954486 | None:intergenic | 30.0% | |
| ! | AGATTTACCTTGTTATCTTC+AGG | - | chr8.1:18954945-18954964 | None:intergenic | 30.0% |
| ! | CAAAATAATCTCTTTAAGGG+TGG | - | chr8.1:18954757-18954776 | None:intergenic | 30.0% |
| ! | GGCCTGGTTATAAATTTTAT+TGG | + | chr8.1:18955277-18955296 | MS.gene56948:intron | 30.0% |
| !! | TAAGAGAGAGATTTGTTGAA+TGG | + | chr8.1:18955379-18955398 | MS.gene56948:intron | 30.0% |
| !!! | TATGTTGTTTTTGGTTTCAG+AGG | + | chr8.1:18954797-18954816 | MS.gene56948:intron | 30.0% |
| !!! | TTTTGAAGTGCATAATGTTG+TGG | - | chr8.1:18954576-18954595 | None:intergenic | 30.0% |
| AACCAAATGGCAAAGAAGAA+AGG | + | chr8.1:18954480-18954499 | MS.gene56948:CDS | 35.0% | |
| AACCTTTCTTCTTTGCCATT+TGG | - | chr8.1:18954485-18954504 | None:intergenic | 35.0% | |
| AATCTGAAAGACATGAGTCA+AGG | + | chr8.1:18955447-18955466 | MS.gene56948:CDS | 35.0% | |
| ACAAAAGCAACAACAAATGG+TGG | + | chr8.1:18955470-18955489 | MS.gene56948:CDS | 35.0% | |
| AGGACAAAAGCAACAACAAA+TGG | + | chr8.1:18955467-18955486 | MS.gene56948:CDS | 35.0% | |
| GCAAAGAAGAAAGGTTGTAT+TGG | + | chr8.1:18954489-18954508 | MS.gene56948:CDS | 35.0% | |
| TACTGTTCATAAGAAGGAGT+TGG | - | chr8.1:18955084-18955103 | None:intergenic | 35.0% | |
| TCAGTGATGCTTTGTAAAGA+GGG | - | chr8.1:18955527-18955546 | None:intergenic | 35.0% | |
| TCTTGCAGATTCAATCAACA+TGG | - | chr8.1:18955007-18955026 | None:intergenic | 35.0% | |
| TTCAGTGATGCTTTGTAAAG+AGG | - | chr8.1:18955528-18955547 | None:intergenic | 35.0% | |
| TTGGTTTCAGAGGTTACAAA+GGG | + | chr8.1:18954807-18954826 | MS.gene56948:intron | 35.0% | |
| ! | TTTGGTTTCAGAGGTTACAA+AGG | + | chr8.1:18954806-18954825 | MS.gene56948:intron | 35.0% |
| ATCACTTCACTAGCAGTAAC+TGG | - | chr8.1:18954515-18954534 | None:intergenic | 40.0% | |
| CCTTGCTACTAGAAAGTCAA+TGG | - | chr8.1:18955329-18955348 | None:intergenic | 40.0% | |
| GTCTGAACCTGAAGATAACA+AGG | + | chr8.1:18954935-18954954 | MS.gene56948:CDS | 40.0% | |
| TATAGACTCATCTCAGCTCA+AGG | + | chr8.1:18954698-18954717 | MS.gene56948:CDS | 40.0% | |
| ! | AGAGGTTACAAAGGGTATGT+GGG | + | chr8.1:18954815-18954834 | MS.gene56948:intron | 40.0% |
| !! | CCATTGACTTTCTAGTAGCA+AGG | + | chr8.1:18955326-18955345 | MS.gene56948:intron | 40.0% |
| !!! | TTTGACTTTTGTGCTGCTTC+TGG | - | chr8.1:18954876-18954895 | None:intergenic | 40.0% |
| AGAAGAGCAACGTAGTGATC+AGG | - | chr8.1:18954548-18954567 | None:intergenic | 45.0% | |
| CCAACAGATACACTCTTGCT+TGG | + | chr8.1:18954668-18954687 | MS.gene56948:CDS | 45.0% | |
| GAAGAGCAACGTAGTGATCA+GGG | - | chr8.1:18954547-18954566 | None:intergenic | 45.0% | |
| ! | CAGAGGTTACAAAGGGTATG+TGG | + | chr8.1:18954814-18954833 | MS.gene56948:intron | 45.0% |
| ! | CCAAGCAAGAGTGTATCTGT+TGG | - | chr8.1:18954671-18954690 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 18954434 | 18955559 | 18954434 | ID=MS.gene56948 |
| chr8.1 | mRNA | 18954434 | 18955559 | 18954434 | ID=MS.gene56948.t1;Parent=MS.gene56948 |
| chr8.1 | exon | 18954434 | 18954719 | 18954434 | ID=MS.gene56948.t1.exon1;Parent=MS.gene56948.t1 |
| chr8.1 | CDS | 18954434 | 18954719 | 18954434 | ID=cds.MS.gene56948.t1;Parent=MS.gene56948.t1 |
| chr8.1 | exon | 18954817 | 18954956 | 18954817 | ID=MS.gene56948.t1.exon2;Parent=MS.gene56948.t1 |
| chr8.1 | CDS | 18954817 | 18954956 | 18954817 | ID=cds.MS.gene56948.t1;Parent=MS.gene56948.t1 |
| chr8.1 | exon | 18955440 | 18955559 | 18955440 | ID=MS.gene56948.t1.exon3;Parent=MS.gene56948.t1 |
| chr8.1 | CDS | 18955440 | 18955559 | 18955440 | ID=cds.MS.gene56948.t1;Parent=MS.gene56948.t1 |
| Gene Sequence |
| Protein sequence |
