AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57468

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57468.t1 MTR_2g035820 99.576 236 1 0 1 236 1 236 2.79e-177 486
MS.gene57468.t1 MTR_0003s0620 51.748 143 68 1 2 144 7 148 4.19e-48 155
MS.gene57468.t1 MTR_0003s0620 36.638 232 103 3 2 233 7 194 1.35e-42 143
MS.gene57468.t1 MTR_8g085220 35.526 152 88 5 14 157 25 174 1.26e-20 90.5
MS.gene57468.t1 MTR_8g085220 35.526 152 88 5 14 157 25 174 1.35e-20 90.5
MS.gene57468.t1 MTR_4g068950 27.174 184 120 6 3 175 15 195 2.98e-19 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57468.t1 AT3G17365 69.038 239 70 3 1 236 1 238 1.16e-122 348
MS.gene57468.t1 AT3G17365 55.235 277 82 6 1 236 1 276 9.18e-101 294
MS.gene57468.t1 AT3G60910 41.921 229 129 3 2 227 7 234 3.87e-63 197
MS.gene57468.t1 AT3G60910 42.197 173 96 3 58 227 1 172 6.85e-44 146
MS.gene57468.t1 AT4G34360 29.444 180 113 7 7 175 14 190 4.98e-20 85.9
MS.gene57468.t1 AT2G31740 31.551 187 113 7 14 188 29 212 2.90e-17 80.9

Find 53 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAGATGCTTGAGGAAATT+TGG 0.165677 2.2:+50762821 MS.gene57468:CDS
TCTATCCTTGTTGTTGGTTC+TGG 0.233828 2.2:+50761651 MS.gene57468:CDS
GTTGTTGGTTCTGGTAATTC+AGG 0.253017 2.2:+50761660 MS.gene57468:CDS
TAATTAGACAGATTTGAAAA+TGG 0.306383 2.2:+50762503 MS.gene57468:intron
CCATCATTATTAAGTGGAAT+AGG 0.346132 2.2:-50763484 None:intergenic
GGGCCAAAGTAATATACTTT+TGG 0.356009 2.2:-50761596 None:intergenic
TAGTGCAAATGTATATATAA+TGG 0.365312 2.2:-50763546 None:intergenic
AGGAACTCTTGATTCTATCT+TGG 0.371492 2.2:+50762691 MS.gene57468:intron
AACAAGGATAGATTGGTTCT+TGG 0.386383 2.2:-50761640 None:intergenic
AACCAATCTATCCTTGTTGT+TGG 0.393670 2.2:+50761645 MS.gene57468:CDS
GGAACATAGAGATTGATGAT+AGG 0.416346 2.2:-50761618 None:intergenic
GGACATCGGGATTCAGTCCT+AGG 0.416500 2.2:-50763525 None:intergenic
ACAAGGATAGATTGGTTCTT+GGG 0.427283 2.2:-50761639 None:intergenic
GGCTAAAGGAGTCTATATTC+TGG 0.446501 2.2:+50762971 MS.gene57468:CDS
CCTATTCCACTTAATAATGA+TGG 0.448893 2.2:+50763484 MS.gene57468:CDS
AACCAACAACAAGGATAGAT+TGG 0.452329 2.2:-50761647 None:intergenic
GAGCTTCCATCATTATTAAG+TGG 0.453928 2.2:-50763490 None:intergenic
GTACCAAAAGTATATTACTT+TGG 0.454668 2.2:+50761593 MS.gene57468:CDS
GAACAAACAGTGATGGCAAC+AGG 0.463928 2.2:+50761501 None:intergenic
GAACATAGAGATTGATGATA+GGG 0.464220 2.2:-50761617 None:intergenic
CATACGGTGAATCATGGTAC+TGG 0.475651 2.2:+50761532 MS.gene57468:CDS
AGCAAACGTAAGCGATATAG+TGG 0.489409 2.2:-50763116 None:intergenic
TGCAGCGTTCAGTCAAGGTA+TGG 0.489551 2.2:+50761878 MS.gene57468:intron
ACAATTAAACTCCATGTGAT+AGG 0.491672 2.2:+50763158 MS.gene57468:CDS
ATGTATATATAATGGACATC+GGG 0.504347 2.2:-50763538 None:intergenic
TTATATATACATTTGCACTA+AGG 0.515855 2.2:+50763549 MS.gene57468:CDS
TATAGGGTTCTCAAGGCTAA+AGG 0.523386 2.2:+50762957 MS.gene57468:intron
CACAAGCATACGGTGAATCA+TGG 0.526996 2.2:+50761526 MS.gene57468:CDS
TTTGGTTCTGTTATTGACAA+AGG 0.530391 2.2:+50762561 MS.gene57468:CDS
GACCAGGTTCATTTGTGTAA+CGG 0.544016 2.2:-50761560 None:intergenic
GGTTGATGAAGGTGGTTACA+AGG 0.548904 2.2:+50761899 MS.gene57468:CDS
AAAATCTGATAATCCACTCT+GGG 0.552145 2.2:+50763450 MS.gene57468:CDS
TTGTTTGCAGCGTTCAGTCA+AGG 0.556939 2.2:+50761873 MS.gene57468:intron
TGGTACCAATCAAAAGGACC+AGG 0.559970 2.2:-50761576 None:intergenic
TAACATTGATATATCTTCAG+TGG 0.564648 2.2:+50761929 MS.gene57468:CDS
ATAAGGATACACTTCAATTG+AGG 0.578933 2.2:-50761987 None:intergenic
AGATGCTTGAGGAAATTTGG+AGG 0.579739 2.2:+50762824 MS.gene57468:CDS
GACACACTATAGGGTTCTCA+AGG 0.580644 2.2:+50762950 MS.gene57468:intron
AGTCAAGGTATGGTTGATGA+AGG 0.581181 2.2:+50761888 MS.gene57468:CDS
AATGTATATATAATGGACAT+CGG 0.584034 2.2:-50763539 None:intergenic
ACTTCAATTGAGGACGATCT+CGG 0.589584 2.2:-50761977 None:intergenic
TGCTACGTGATTCATGCTCG+TGG 0.598791 2.2:+50763135 MS.gene57468:CDS
AACCGTTACACAAATGAACC+TGG 0.604182 2.2:+50761558 MS.gene57468:CDS
GACATCGGGATTCAGTCCTA+GGG 0.605591 2.2:-50763524 None:intergenic
CAAGGTATGGTTGATGAAGG+TGG 0.609881 2.2:+50761891 MS.gene57468:CDS
ATACGGTGAATCATGGTACT+GGG 0.624246 2.2:+50761533 MS.gene57468:CDS
ACATGCTACTAAGATGCTTG+AGG 0.629503 2.2:+50762813 MS.gene57468:CDS
GCAACAGGTACACAAGCATA+CGG 0.630715 2.2:+50761516 MS.gene57468:CDS
AACATAGAGATTGATGATAG+GGG 0.639887 2.2:-50761616 None:intergenic
AGCTCTGTGGATGAAACCCT+AGG 0.652280 2.2:+50763508 MS.gene57468:CDS
TAATAATGATGGAAGCTCTG+TGG 0.664960 2.2:+50763495 MS.gene57468:CDS
ATTTAAACCAACCTATCACA+TGG 0.671256 2.2:-50763169 None:intergenic
AATTACCAGAACCAACAACA+AGG 0.677098 2.2:-50761656 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAACAATATTAATAAAAT+GGG + chr2.2:50764703-50764722 MS.gene57468:intron 10.0%
!! TATCAACAATATTAATAAAA+TGG + chr2.2:50764702-50764721 MS.gene57468:intron 10.0%
!!! AAAACAATATATTTTTACAA+AGG - chr2.2:50762323-50762342 None:intergenic 10.0%
!!! AAATGTTTTTTTTAGAAAAA+TGG - chr2.2:50763269-50763288 None:intergenic 10.0%
!!! CTAATGTTTTTTTTTTTTTT+TGG - chr2.2:50761695-50761714 None:intergenic 10.0%
!! ATAAATATGATTCTCATATA+TGG + chr2.2:50763964-50763983 MS.gene57468:intron 15.0%
!!! AATCTTTAATTTTGTTTTTC+AGG + chr2.2:50762671-50762690 MS.gene57468:intron 15.0%
!!! TTTTATTATTCTACATTGAA+GGG - chr2.2:50763771-50763790 None:intergenic 15.0%
!!! TTTTTATTATTCTACATTGA+AGG - chr2.2:50763772-50763791 None:intergenic 15.0%
!! AACATGAATTAATCAACATA+AGG - chr2.2:50762007-50762026 None:intergenic 20.0%
!! AACTGCATAATTTAAAAACA+TGG - chr2.2:50762035-50762054 None:intergenic 20.0%
!! AATATTAATAAAATGGGAGA+AGG + chr2.2:50764709-50764728 MS.gene57468:intron 20.0%
!! ATTTGAATAATTAATCGTCA+TGG + chr2.2:50765535-50765554 MS.gene57468:intron 20.0%
!! CGATTAATTATTCAAATTAG+CGG - chr2.2:50765532-50765551 None:intergenic 20.0%
!! GTATTTATGATTCTATAACA+TGG - chr2.2:50762415-50762434 None:intergenic 20.0%
!! TAATTAGACAGATTTGAAAA+TGG + chr2.2:50762503-50762522 MS.gene57468:intron 20.0%
!! TAGTGCAAATGTATATATAA+TGG - chr2.2:50763549-50763568 None:intergenic 20.0%
!! TCAAATCTGTCTAATTATTA+AGG - chr2.2:50762500-50762519 None:intergenic 20.0%
!! TTATATATACATTTGCACTA+AGG + chr2.2:50763549-50763568 MS.gene57468:CDS 20.0%
!!! AAAAAATTTAGTTTCCTAAC+AGG + chr2.2:50764220-50764239 MS.gene57468:intron 20.0%
!!! AATGTATATATAATGGACAT+CGG - chr2.2:50763542-50763561 None:intergenic 20.0%
!!! AATTTTGTTGGCTATTTTAT+AGG - chr2.2:50764280-50764299 None:intergenic 20.0%
!!! AGAATTTCTTTTATAAAGAG+AGG - chr2.2:50765207-50765226 None:intergenic 20.0%
!!! ATGTTTTTAAATTATGCAGT+TGG + chr2.2:50762034-50762053 MS.gene57468:intron 20.0%
!!! GAATTTCTTTTATAAAGAGA+GGG - chr2.2:50765206-50765225 None:intergenic 20.0%
!!! GTACAAAAGTATTTATATCA+CGG + chr2.2:50762273-50762292 MS.gene57468:intron 20.0%
!!! TAGTATTTCTAGTTTGATTT+TGG + chr2.2:50762175-50762194 MS.gene57468:intron 20.0%
!!! TTTATAAAATTTTGCTGAAG+TGG + chr2.2:50762438-50762457 MS.gene57468:intron 20.0%
!!! TTTTAGATTTACTTTTGACT+TGG + chr2.2:50764904-50764923 MS.gene57468:intron 20.0%
!!! TTTTTTAGATCTCTCTTATA+TGG + chr2.2:50761722-50761741 MS.gene57468:intron 20.0%
! AAAAGACATGTTTCATCTAT+TGG - chr2.2:50763039-50763058 None:intergenic 25.0%
! AAAATCTTCTTCACTCTAAA+GGG - chr2.2:50764989-50765008 None:intergenic 25.0%
! AATCCAAAATGAAATAGAAG+TGG - chr2.2:50762888-50762907 None:intergenic 25.0%
! AGAAGAAACACAGTAATAAA+GGG - chr2.2:50764795-50764814 None:intergenic 25.0%
! ATAGAAGTGGTAAAAAACTA+TGG - chr2.2:50762875-50762894 None:intergenic 25.0%
! ATATGAGTCTATGAATGAAT+GGG - chr2.2:50762594-50762613 None:intergenic 25.0%
! ATGTGATTTATTTACTACGA+TGG + chr2.2:50764852-50764871 MS.gene57468:intron 25.0%
! ATTGATATGATATCAAGGTT+TGG - chr2.2:50764049-50764068 None:intergenic 25.0%
! CAAAAAATGGCAATAATAAG+AGG - chr2.2:50763195-50763214 None:intergenic 25.0%
! CAGATGAAAAAGAATAAATG+GGG - chr2.2:50761760-50761779 None:intergenic 25.0%
! CCAGATGAAAAAGAATAAAT+GGG - chr2.2:50761761-50761780 None:intergenic 25.0%
! GAGTAAATCTGAATACAAAT+CGG - chr2.2:50763015-50763034 None:intergenic 25.0%
! GTAGAATATAATGTACCTTT+AGG + chr2.2:50764479-50764498 MS.gene57468:intron 25.0%
! TAACATTGATATATCTTCAG+TGG + chr2.2:50761929-50761948 MS.gene57468:CDS 25.0%
! TATATGAGTCTATGAATGAA+TGG - chr2.2:50762595-50762614 None:intergenic 25.0%
! TATTTCATTTCCTACAGAAA+AGG - chr2.2:50761840-50761859 None:intergenic 25.0%
! TCATCTTGTTGAAAATACTT+AGG + chr2.2:50765393-50765412 MS.gene57468:intron 25.0%
! TCTATAACATGGATATAACA+TGG - chr2.2:50762404-50762423 None:intergenic 25.0%
! TTAAAGATTATCAGAACGAT+TGG - chr2.2:50762660-50762679 None:intergenic 25.0%
! TTAGACAACTCTCAAAAAAA+AGG - chr2.2:50763652-50763671 None:intergenic 25.0%
! TTATAAAGGAACACGTAAAT+AGG - chr2.2:50763305-50763324 None:intergenic 25.0%
! TTCAACTAGATTATCAACAA+TGG - chr2.2:50764677-50764696 None:intergenic 25.0%
! TTTAATCAGTGTCATGTAAA+TGG + chr2.2:50764457-50764476 MS.gene57468:intron 25.0%
!! GTACCAAAAGTATATTACTT+TGG + chr2.2:50761593-50761612 MS.gene57468:CDS 25.0%
!! TACTTACTCTTTTATGTTCT+TGG + chr2.2:50763590-50763609 MS.gene57468:intron 25.0%
!! TCATAAATTTTACCAAACGA+AGG + chr2.2:50763672-50763691 MS.gene57468:intron 25.0%
!! TGAAAATACTTAGGAGTATA+TGG + chr2.2:50765402-50765421 MS.gene57468:intron 25.0%
!! TTACCACTTCTATTTCATTT+TGG + chr2.2:50762882-50762901 MS.gene57468:intron 25.0%
!! TTTATAAATTTGCTCCTCTT+CGG + chr2.2:50763318-50763337 MS.gene57468:intron 25.0%
!!! ATATTTTTGTTGTACTAGTG+TGG + chr2.2:50762775-50762794 MS.gene57468:intron 25.0%
!!! ATGTATATATAATGGACATC+GGG - chr2.2:50763541-50763560 None:intergenic 25.0%
!!! CCATTTATTCTTTTTCATCT+GGG + chr2.2:50761759-50761778 MS.gene57468:intron 25.0%
!!! TTTTTTATCACCTTTTCTGT+AGG + chr2.2:50761827-50761846 MS.gene57468:intron 25.0%
AAAAATCTGATAATCCACTC+TGG + chr2.2:50763449-50763468 MS.gene57468:CDS 30.0%
AAAATCTGATAATCCACTCT+GGG + chr2.2:50763450-50763469 MS.gene57468:CDS 30.0%
AAGACATCAAAGTGTATGTT+TGG + chr2.2:50762124-50762143 MS.gene57468:intron 30.0%
ACAATTAAACTCCATGTGAT+AGG + chr2.2:50763158-50763177 MS.gene57468:CDS 30.0%
ACTAATGCACAATTTCACTT+CGG - chr2.2:50765038-50765057 None:intergenic 30.0%
ATAAGGATACACTTCAATTG+AGG - chr2.2:50761990-50762009 None:intergenic 30.0%
ATGAAAAAGAATAAATGGGG+TGG - chr2.2:50761757-50761776 None:intergenic 30.0%
ATTATTCAAATTAGCGGCAA+TGG - chr2.2:50765526-50765545 None:intergenic 30.0%
ATTGCATGTTAAGCTTAGAA+GGG - chr2.2:50764648-50764667 None:intergenic 30.0%
ATTGCTATGATATCAAGGTT+TGG - chr2.2:50763896-50763915 None:intergenic 30.0%
ATTTAAACCAACCTATCACA+TGG - chr2.2:50763172-50763191 None:intergenic 30.0%
ATTTGCGGATTTCCTATAAT+AGG + chr2.2:50764304-50764323 MS.gene57468:intron 30.0%
CAAAATCTTCTTCACTCTAA+AGG - chr2.2:50764990-50765009 None:intergenic 30.0%
CCAACAAAATTATGCATTTG+CGG + chr2.2:50764289-50764308 MS.gene57468:intron 30.0%
CCATCATTATTAAGTGGAAT+AGG - chr2.2:50763487-50763506 None:intergenic 30.0%
CCCAGATGAAAAAGAATAAA+TGG - chr2.2:50761762-50761781 None:intergenic 30.0%
CCTATTCCACTTAATAATGA+TGG + chr2.2:50763484-50763503 MS.gene57468:CDS 30.0%
CCTATTTAGACTAATCATTC+TGG + chr2.2:50765610-50765629 MS.gene57468:intron 30.0%
GAAGAGGAGCAAATTTATAA+AGG - chr2.2:50763319-50763338 None:intergenic 30.0%
GAGAAGAAACACAGTAATAA+AGG - chr2.2:50764796-50764815 None:intergenic 30.0%
GCACAATTGATATGATATCA+AGG - chr2.2:50764054-50764073 None:intergenic 30.0%
GCTTGTTCTTATAGAATGAT+TGG - chr2.2:50764532-50764551 None:intergenic 30.0%
TGATTTATTTACTACGATGG+TGG + chr2.2:50764855-50764874 MS.gene57468:intron 30.0%
TTAGTCCCTAAAAGCAAATT+TGG + chr2.2:50764571-50764590 MS.gene57468:intron 30.0%
TTATTCAAATTAGCGGCAAT+GGG - chr2.2:50765525-50765544 None:intergenic 30.0%
TTGCATGAATTAGAAAGTCA+TGG - chr2.2:50764356-50764375 None:intergenic 30.0%
TTTGCGGATTTCCTATAATA+GGG + chr2.2:50764305-50764324 MS.gene57468:intron 30.0%
! AACATAGAGATTGATGATAG+GGG - chr2.2:50761619-50761638 None:intergenic 30.0%
! AACTATGGACTTTTTGTTCA+TGG - chr2.2:50762860-50762879 None:intergenic 30.0%
! ACTTTTGTACACGTTTAGTT+TGG - chr2.2:50762263-50762282 None:intergenic 30.0%
! GAACATAGAGATTGATGATA+GGG - chr2.2:50761620-50761639 None:intergenic 30.0%
! GATTTTCCACTTCAAATTTG+CGG - chr2.2:50763749-50763768 None:intergenic 30.0%
! TACTTTTGGTACCAATCAAA+AGG - chr2.2:50761585-50761604 None:intergenic 30.0%
! TCTTTACAATGGTGGAAATA+TGG + chr2.2:50765085-50765104 MS.gene57468:intron 30.0%
! TGTTAACTTGGCACAAAAAA+TGG - chr2.2:50763208-50763227 None:intergenic 30.0%
! TTCTTTTATAAAGAGAGGGA+AGG - chr2.2:50765202-50765221 None:intergenic 30.0%
!! ATCCAACCAAATTTGCTTTT+AGG - chr2.2:50764580-50764599 None:intergenic 30.0%
!! CCAGAATGATTAGTCTAAAT+AGG - chr2.2:50765613-50765632 None:intergenic 30.0%
!! CCCATTTATTCTTTTTCATC+TGG + chr2.2:50761758-50761777 MS.gene57468:intron 30.0%
!! TCCAACCAAATTTGCTTTTA+GGG - chr2.2:50764579-50764598 None:intergenic 30.0%
!! TGATTAGTCTAAATAGGCAA+AGG - chr2.2:50765607-50765626 None:intergenic 30.0%
!! TTTCTTCTCCTTTTGAAAGT+TGG + chr2.2:50764807-50764826 MS.gene57468:intron 30.0%
!! TTTGGTTCTGTTATTGACAA+AGG + chr2.2:50762561-50762580 MS.gene57468:CDS 30.0%
!!! TTCGGTTTTCTAGTTTCTAT+CGG - chr2.2:50765020-50765039 None:intergenic 30.0%
AACCAACAACAAGGATAGAT+TGG - chr2.2:50761650-50761669 None:intergenic 35.0%
AAGAAATTCTCCTAGACTTC+AGG + chr2.2:50765217-50765236 MS.gene57468:intron 35.0%
AAGATAAGCATAACGAAGAG+TGG - chr2.2:50763997-50764016 None:intergenic 35.0%
AAGATTGAAGAAGAAGCTGA+AGG - chr2.2:50765265-50765284 None:intergenic 35.0%
AAGTATTTATATCACGGCTC+AGG + chr2.2:50762279-50762298 MS.gene57468:intron 35.0%
AAGTTTCTCTGCACATATCA+CGG - chr2.2:50763419-50763438 None:intergenic 35.0%
AATGATTGGAAACTCACGAA+TGG - chr2.2:50764518-50764537 None:intergenic 35.0%
AATTACCAGAACCAACAACA+AGG - chr2.2:50761659-50761678 None:intergenic 35.0%
ACGTAAATAGGATGCTTCAA+AGG - chr2.2:50763293-50763312 None:intergenic 35.0%
ACTCCAGATATGAATGAACT+AGG - chr2.2:50763367-50763386 None:intergenic 35.0%
AGAAATTCTCCTAGACTTCA+GGG + chr2.2:50765218-50765237 MS.gene57468:intron 35.0%
AGAAGAAGCTGAAGGAATAA+AGG - chr2.2:50765257-50765276 None:intergenic 35.0%
AGGAACTCTTGATTCTATCT+TGG + chr2.2:50762691-50762710 MS.gene57468:intron 35.0%
ATGTTCTTGGTCAATAGTGT+TGG + chr2.2:50763603-50763622 MS.gene57468:intron 35.0%
CATTGCATGTTAAGCTTAGA+AGG - chr2.2:50764649-50764668 None:intergenic 35.0%
CGTAAATAGGATGCTTCAAA+GGG - chr2.2:50763292-50763311 None:intergenic 35.0%
CTAAGATGCTTGAGGAAATT+TGG + chr2.2:50762821-50762840 MS.gene57468:CDS 35.0%
CTAAGCTTAACATGCAATGA+AGG + chr2.2:50764650-50764669 MS.gene57468:intron 35.0%
CTGAAGGAATAAAGGTTGTT+AGG - chr2.2:50765249-50765268 None:intergenic 35.0%
GAATACAAATCGGTACTAAG+AGG - chr2.2:50763005-50763024 None:intergenic 35.0%
GAGCTTCCATCATTATTAAG+TGG - chr2.2:50763493-50763512 None:intergenic 35.0%
GATTCAGTGAAATTGCCTTA+GGG - chr2.2:50763944-50763963 None:intergenic 35.0%
GCACAATTGCTATGATATCA+AGG - chr2.2:50763901-50763920 None:intergenic 35.0%
GGTTCTACATATGTTCCTAA+AGG - chr2.2:50764497-50764516 None:intergenic 35.0%
GTCATCAACTGCTTCAATAA+TGG - chr2.2:50762933-50762952 None:intergenic 35.0%
GTGGAAGGAATCAACTAATA+AGG - chr2.2:50764407-50764426 None:intergenic 35.0%
TCCCTAAAAGCAAATTTGGT+TGG + chr2.2:50764575-50764594 MS.gene57468:intron 35.0%
TCCTCTTCGGTCAAATATAT+AGG + chr2.2:50763331-50763350 MS.gene57468:intron 35.0%
TCTGGTATAATCTTTCTGAC+AGG + chr2.2:50765628-50765647 MS.gene57468:intron 35.0%
TGAAGGAGAGTCAAAACATT+TGG - chr2.2:50764185-50764204 None:intergenic 35.0%
TGAATGCGTGCAAAAATTTC+AGG + chr2.2:50763079-50763098 MS.gene57468:intron 35.0%
TGAGATGTGTTCCCTATTAT+AGG - chr2.2:50764319-50764338 None:intergenic 35.0%
TGATTCAGTGAAATTGCCTT+AGG - chr2.2:50763945-50763964 None:intergenic 35.0%
TGCATTAGTGACTCTGATAA+TGG + chr2.2:50765049-50765068 MS.gene57468:intron 35.0%
TTAAACTCCATGTGATAGGT+TGG + chr2.2:50763162-50763181 MS.gene57468:intron 35.0%
TTGCCTAGTTCATTCATATC+TGG + chr2.2:50763361-50763380 MS.gene57468:intron 35.0%
TTGTTAAAGTTGCCTTCGTT+TGG - chr2.2:50763687-50763706 None:intergenic 35.0%
! AACAAGGATAGATTGGTTCT+TGG - chr2.2:50761643-50761662 None:intergenic 35.0%
! AACCAATCTATCCTTGTTGT+TGG + chr2.2:50761645-50761664 MS.gene57468:CDS 35.0%
! AAGACTAACAGTTTGCTCAT+TGG + chr2.2:50762752-50762771 MS.gene57468:intron 35.0%
! AAGTACTGCCAACTTTCAAA+AGG - chr2.2:50764818-50764837 None:intergenic 35.0%
! ACAAGGATAGATTGGTTCTT+GGG - chr2.2:50761642-50761661 None:intergenic 35.0%
! CAAAAATTTCAGGTGACGTA+TGG + chr2.2:50763089-50763108 MS.gene57468:intron 35.0%
! CCGCAAATGCATAATTTTGT+TGG - chr2.2:50764292-50764311 None:intergenic 35.0%
! GGAACATAGAGATTGATGAT+AGG - chr2.2:50761621-50761640 None:intergenic 35.0%
! GGGCCAAAGTAATATACTTT+TGG - chr2.2:50761599-50761618 None:intergenic 35.0%
! TAATAATGATGGAAGCTCTG+TGG + chr2.2:50763495-50763514 MS.gene57468:CDS 35.0%
! TGATGATAGTGATGTTCTTG+AGG + chr2.2:50762465-50762484 MS.gene57468:intron 35.0%
! TTCTTCACTCTAAAGGGTAT+TGG - chr2.2:50764983-50765002 None:intergenic 35.0%
!! TTTGACTCGATGCATTTTGA+AGG - chr2.2:50764202-50764221 None:intergenic 35.0%
!! TTTGTACACGTTTAGTTTGG+TGG - chr2.2:50762260-50762279 None:intergenic 35.0%
!!! TTTTTTGAAGATAGCCAGTG+AGG + chr2.2:50763803-50763822 MS.gene57468:intron 35.0%
AACCGTTACACAAATGAACC+TGG + chr2.2:50761558-50761577 MS.gene57468:CDS 40.0%
ACATGCTACTAAGATGCTTG+AGG + chr2.2:50762813-50762832 MS.gene57468:CDS 40.0%
ACTTCAATTGAGGACGATCT+CGG - chr2.2:50761980-50761999 None:intergenic 40.0%
AGATGCTTGAGGAAATTTGG+AGG + chr2.2:50762824-50762843 MS.gene57468:CDS 40.0%
AGATTGCCGCAAATTTGAAG+TGG + chr2.2:50763740-50763759 MS.gene57468:intron 40.0%
AGCAAACGTAAGCGATATAG+TGG - chr2.2:50763119-50763138 None:intergenic 40.0%
ATACGGTGAATCATGGTACT+GGG + chr2.2:50761533-50761552 MS.gene57468:CDS 40.0%
CTTGAAGCTCTTTACAATGG+TGG + chr2.2:50765077-50765096 MS.gene57468:intron 40.0%
GAAACTGCACTAGTGTGAAT+TGG + chr2.2:50765288-50765307 MS.gene57468:intron 40.0%
GAACCATGCATGTGTTAACT+TGG - chr2.2:50763220-50763239 None:intergenic 40.0%
GACCAGGTTCATTTGTGTAA+CGG - chr2.2:50761563-50761582 None:intergenic 40.0%
GAGCTTGAAGCTCTTTACAA+TGG + chr2.2:50765074-50765093 MS.gene57468:intron 40.0%
GCCTATATATTTGACCGAAG+AGG - chr2.2:50763335-50763354 None:intergenic 40.0%
GGCTAAAGGAGTCTATATTC+TGG + chr2.2:50762971-50762990 MS.gene57468:CDS 40.0%
GTCTCAAAATTCTCTCCCTA+AGG + chr2.2:50763926-50763945 MS.gene57468:intron 40.0%
TAAGCATAACGAAGAGTGGA+CGG - chr2.2:50763993-50764012 None:intergenic 40.0%
TAGAAATACTACAGCGATCC+TGG - chr2.2:50762166-50762185 None:intergenic 40.0%
TAGGATATACCCTGAAGTCT+AGG - chr2.2:50765230-50765249 None:intergenic 40.0%
TATAGGGTTCTCAAGGCTAA+AGG + chr2.2:50762957-50762976 MS.gene57468:intron 40.0%
TCAAGTAAGTCCTCCTAAGT+AGG - chr2.2:50763844-50763863 None:intergenic 40.0%
! AGGAAACTGATAGAGCATCA+GGG - chr2.2:50765345-50765364 None:intergenic 40.0%
! ATGAACCTGGTCCTTTTGAT+TGG + chr2.2:50761571-50761590 MS.gene57468:CDS 40.0%
! GCTTTTGCATCAGAGACTTT+TGG + chr2.2:50762543-50762562 MS.gene57468:CDS 40.0%
!! AGCAGTTGATGACACACTAT+AGG + chr2.2:50762940-50762959 MS.gene57468:intron 40.0%
!! AGTCAAGGTATGGTTGATGA+AGG + chr2.2:50761888-50761907 MS.gene57468:CDS 40.0%
!! GCAGTTGATGACACACTATA+GGG + chr2.2:50762941-50762960 MS.gene57468:intron 40.0%
!! GTTGTTGGTTCTGGTAATTC+AGG + chr2.2:50761660-50761679 MS.gene57468:CDS 40.0%
!! GTTTAGTTTGGTGGTGAGTT+TGG - chr2.2:50762251-50762270 None:intergenic 40.0%
!! TCTATCCTTGTTGTTGGTTC+TGG + chr2.2:50761651-50761670 MS.gene57468:CDS 40.0%
!!! GAGTGTTTTAACGAGATGAG+AGG - chr2.2:50765365-50765384 None:intergenic 40.0%
ATCAGGGTCCAGTAAATGTG+AGG - chr2.2:50765329-50765348 None:intergenic 45.0%
CACAAGCATACGGTGAATCA+TGG + chr2.2:50761526-50761545 MS.gene57468:CDS 45.0%
CATACGGTGAATCATGGTAC+TGG + chr2.2:50761532-50761551 MS.gene57468:CDS 45.0%
CTTATGAGAGGATCTCTCTC+TGG - chr2.2:50765136-50765155 None:intergenic 45.0%
GACACACTATAGGGTTCTCA+AGG + chr2.2:50762950-50762969 MS.gene57468:intron 45.0%
GCAACAGGTACACAAGCATA+CGG + chr2.2:50761516-50761535 MS.gene57468:CDS 45.0%
GCACGCATTCAAGATCGATT+AGG - chr2.2:50763070-50763089 None:intergenic 45.0%
GGTCTCATTTGAGTCGTGAT+GGG - chr2.2:50763875-50763894 None:intergenic 45.0%
GTCTCATTTGAGTCGTGATG+GGG - chr2.2:50763874-50763893 None:intergenic 45.0%
GTGCCAAGTTAACACATGCA+TGG + chr2.2:50763214-50763233 MS.gene57468:intron 45.0%
TCACTCCTACAGTCCTACTT+AGG + chr2.2:50763828-50763847 MS.gene57468:intron 45.0%
TCAGGGTCCAGTAAATGTGA+GGG - chr2.2:50765328-50765347 None:intergenic 45.0%
TGAAGAATCACTCGCCTGTT+AGG - chr2.2:50764237-50764256 None:intergenic 45.0%
TGGTACCAATCAAAAGGACC+AGG - chr2.2:50761579-50761598 None:intergenic 45.0%
TGGTCTCATTTGAGTCGTGA+GGG - chr2.2:50763876-50763895 None:intergenic 45.0%
TGGTCTCATTTGAGTCGTGA+TGG - chr2.2:50763876-50763895 None:intergenic 45.0%
TGTGAATTGGACCCGATGAT+GGG + chr2.2:50765301-50765320 MS.gene57468:intron 45.0%
TTAAGACGACGTTCGTGTAG+TGG - chr2.2:50764426-50764445 None:intergenic 45.0%
TTGGTCTCATTTGAGTCGTG+AGG - chr2.2:50763877-50763896 None:intergenic 45.0%
TTGTTTGCAGCGTTCAGTCA+AGG + chr2.2:50761873-50761892 MS.gene57468:intron 45.0%
! GAGGAAACTGATAGAGCATC+AGG - chr2.2:50765346-50765365 None:intergenic 45.0%
! TTATATCACGGCTCAGGTTG+CGG + chr2.2:50762285-50762304 MS.gene57468:intron 45.0%
!! CAAGGTATGGTTGATGAAGG+TGG + chr2.2:50761891-50761910 MS.gene57468:CDS 45.0%
!! GGTTGATGAAGGTGGTTACA+AGG + chr2.2:50761899-50761918 MS.gene57468:CDS 45.0%
AAATGTGAGGGGCCCATCAT+CGG - chr2.2:50765316-50765335 None:intergenic 50.0%
AGAGGATCTCTCTCTGGTCT+TGG - chr2.2:50765130-50765149 None:intergenic 50.0%
AGCTCTGTGGATGAAACCCT+AGG + chr2.2:50763508-50763527 MS.gene57468:CDS 50.0%
CAGGGTCCAGTAAATGTGAG+GGG - chr2.2:50765327-50765346 None:intergenic 50.0%
CGCGTCACTTCACTTATGAG+AGG - chr2.2:50765148-50765167 None:intergenic 50.0%
CTCCTACAGTCCTACTTAGG+AGG + chr2.2:50763831-50763850 MS.gene57468:intron 50.0%
CTCTGCACATATCACGGAAG+TGG - chr2.2:50763413-50763432 None:intergenic 50.0%
GACATCGGGATTCAGTCCTA+GGG - chr2.2:50763527-50763546 None:intergenic 50.0%
GGTCTCATTTGAGTCGTGAG+GGG - chr2.2:50764028-50764047 None:intergenic 50.0%
GTCCTCCTAAGTAGGACTGT+AGG - chr2.2:50763836-50763855 None:intergenic 50.0%
GTGTGAATTGGACCCGATGA+TGG + chr2.2:50765300-50765319 MS.gene57468:intron 50.0%
TCTCGAGAGAGAAGCAAGAC+AGG - chr2.2:50764952-50764971 None:intergenic 50.0%
TGCAGCGTTCAGTCAAGGTA+TGG + chr2.2:50761878-50761897 MS.gene57468:intron 50.0%
TGCTACGTGATTCATGCTCG+TGG + chr2.2:50763135-50763154 MS.gene57468:CDS 50.0%
! GGATTCACAGCGATTTTGCC+AGG + chr2.2:50762145-50762164 MS.gene57468:intron 50.0%
AATGTGAGGGGCCCATCATC+GGG - chr2.2:50765315-50765334 None:intergenic 55.0%
CTGTAGGAGTGACACCTCAC+TGG - chr2.2:50763820-50763839 None:intergenic 55.0%
GATGGGCCCCTCACATTTAC+TGG + chr2.2:50765318-50765337 MS.gene57468:intron 55.0%
GGACATCGGGATTCAGTCCT+AGG - chr2.2:50763528-50763547 None:intergenic 55.0%
! GACGACGTTCGTGTAGTGGA+AGG - chr2.2:50764422-50764441 None:intergenic 55.0%
!! GGTTTTGTCAGCTCCCAGAG+TGG - chr2.2:50763466-50763485 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 50761513 50765682 50761513 ID=MS.gene57468
chr2.2 mRNA 50761513 50765682 50761513 ID=MS.gene57468.t1;Parent=MS.gene57468
chr2.2 exon 50761513 50761681 50761513 ID=MS.gene57468.t1.exon1;Parent=MS.gene57468.t1
chr2.2 CDS 50761513 50761681 50761513 ID=cds.MS.gene57468.t1;Parent=MS.gene57468.t1
chr2.2 exon 50761883 50761999 50761883 ID=MS.gene57468.t1.exon2;Parent=MS.gene57468.t1
chr2.2 CDS 50761883 50761999 50761883 ID=cds.MS.gene57468.t1;Parent=MS.gene57468.t1
chr2.2 exon 50762514 50762582 50762514 ID=MS.gene57468.t1.exon3;Parent=MS.gene57468.t1
chr2.2 CDS 50762514 50762582 50762514 ID=cds.MS.gene57468.t1;Parent=MS.gene57468.t1
chr2.2 exon 50762693 50762712 50762693 ID=MS.gene57468.t1.exon4;Parent=MS.gene57468.t1
chr2.2 CDS 50762693 50762712 50762693 ID=cds.MS.gene57468.t1;Parent=MS.gene57468.t1
chr2.2 exon 50762793 50762845 50762793 ID=MS.gene57468.t1.exon5;Parent=MS.gene57468.t1
chr2.2 CDS 50762793 50762845 50762793 ID=cds.MS.gene57468.t1;Parent=MS.gene57468.t1
chr2.2 exon 50762962 50762992 50762962 ID=MS.gene57468.t1.exon6;Parent=MS.gene57468.t1
chr2.2 CDS 50762962 50762992 50762962 ID=cds.MS.gene57468.t1;Parent=MS.gene57468.t1
chr2.2 exon 50763101 50763179 50763101 ID=MS.gene57468.t1.exon7;Parent=MS.gene57468.t1
chr2.2 CDS 50763101 50763179 50763101 ID=cds.MS.gene57468.t1;Parent=MS.gene57468.t1
chr2.2 exon 50763431 50763570 50763431 ID=MS.gene57468.t1.exon8;Parent=MS.gene57468.t1
chr2.2 CDS 50763431 50763570 50763431 ID=cds.MS.gene57468.t1;Parent=MS.gene57468.t1
chr2.2 exon 50765650 50765682 50765650 ID=MS.gene57468.t1.exon9;Parent=MS.gene57468.t1
chr2.2 CDS 50765650 50765682 50765650 ID=cds.MS.gene57468.t1;Parent=MS.gene57468.t1
Gene Sequence

>MS.gene57468

ATGGCAACAGGTACACAAGCATACGGTGAATCATGGTACTGGGACAACCGTTACACAAATGAACCTGGTCCTTTTGATTGGTACCAAAAGTATATTACTTTGGCCCCTATCATCAATCTCTATGTTCCCAAGAACCAATCTATCCTTGTTGTTGGTTCTGGTAATTCAGGTAAGTCAATCCAAAAAAAAAAAAAAACATTAGTTTTTCATTTTTTAGATCTCTCTTATATGGTATTTAATGCCACCCCATTTATTCTTTTTCATCTGGGTTCAATAAAAATCTATTTTTTTCTTCTTGTTTTTAAATATTTTGTTTTTTTATCACCTTTTCTGTAGGAAATGAAATATGTTGTGTTTGTGTTGTTTGCAGCGTTCAGTCAAGGTATGGTTGATGAAGGTGGTTACAAGGATGTTGTTAACATTGATATATCTTCAGTGGTCATTGATGCTATGCAGAAGAAATACCGAGATCGTCCTCAATTGAAGTGTATCCTTATGTTGATTAATTCATGTTATATACCATGTTTTTAAATTATGCAGTTGGATCTTAGTTTTTCATATATTATATTTATAGAGAATTGTAGTCAAATGCTGCTTATATCGTGATCACAAAGACATCAAAGTGTATGTTTGGATTCACAGCGATTTTGCCAGGATCGCTGTAGTATTTCTAGTTTGATTTTGGCAAAATCTATACTTTGATGTTTCAACAAGATCATAGCTAGTGTGATCTTGCCAAACTCACCACCAAACTAAACGTGTACAAAAGTATTTATATCACGGCTCAGGTTGCGGCTCTTTATAAAACCTTTGTAAAAATATATTGTTTTTTAATTTTCACGCTACTACAATTGTCATTAGAAGAAGTATGCATTATTTTACAATTTTCCATGTTATATCCATGTTATAGAATCATAAATACAATTTTATAAAATTTTGCTGAAGTGGTGAATGATGATAGTGATGTTCTTGAGGCTGAAACAACCTTAATAATTAGACAGATTTGAAAATGGATGTCAGAGACATGAGTGCTTTTGCATCAGAGACTTTTGGTTCTGTTATTGACAAAGGTGTGCTTCCCATTCATTCATAGACTCATATATTTTTCTTAGTAAATAATATAATTAGATTAACTGTTGAACAACCAATCGTTCTGATAATCTTTAATTTTGTTTTTCAGGAACTCTTGATTCTATCTTGGTGAGTGATGATGTTCATTGAAATACTTGTATGTTTGACAAGACTAACAGTTTGCTCATTGGATATTTTTGTTGTACTAGTGTGGAAACAATTCAAGACAACATGCTACTAAGATGCTTGAGGAAATTTGGAGGTATAATGAAACCATGAACAAAAAGTCCATAGTTTTTTACCACTTCTATTTCATTTTGGATTTTTTTTATTCTTGATCTGTTCACCATTATTGAAGCAGTTGATGACACACTATAGGGTTCTCAAGGCTAAAGGAGTCTATATTCTGGTAACTGAACCTCTTAGTACCGATTTGTATTCAGATTTACTCTCCAATAGATGAAACATGTCTTTTGCATTTCGCCTAATCGATCTTGAATGCGTGCAAAAATTTCAGGTGACGTATGGAGCTCCACTATATCGCTTACGTTTGCTACGTGATTCATGCTCGTGGACAATTAAACTCCATGTGATAGGTTGGTTTAAATCCTCTTATTATTGCCATTTTTTGTGCCAAGTTAACACATGCATGGTTCTTATATTATATCTTTAATAATATATACCATTTTTCTAAAAAAAACATTTCCCTTTGAAGCATCCTATTTACGTGTTCCTTTATAAATTTGCTCCTCTTCGGTCAAATATATAGGCATTGTTTTGCCTAGTTCATTCATATCTGGAGTGTTAATTAAGTCATTGTCAATAACCACTTCCGTGATATGTGCAGAGAAACTTGCATCAGAAGAAAAATCTGATAATCCACTCTGGGAGCTGACAAAACCTATTCCACTTAATAATGATGGAAGCTCTGTGGATGAAACCCTAGGACTGAATCCCGATGTCCATTATATATACATTTGCACTAAGGTAATCAGATGATTTTTCGTACTTACTCTTTTATGTTCTTGGTCAATAGTGTTGGTATAAAGAGCTAAAACATTATTGCCTTTTTTTTGAGAGTTGTCTAATCATAAATTTTACCAAACGAAGGCAACTTTAACAATATTAGCAATAAAAAGTTGTAGAAAAATAAATAAGATTGCCGCAAATTTGAAGTGGAAAATCCCTTCAATGTAGAATAATAAAAATCACTAGATTCTTTTTTGAAGATAGCCAGTGAGGTGTCACTCCTACAGTCCTACTTAGGAGGACTTACTTGACAAATCTCCCCATCACGACTCAAATGAGACCAAACCTTGATATCATAGCAATTGTGCAACAAGTCTCAAAATTCTCTCCCTAAGGCAATTTCACTGAATCATAAATATGATTCTCATATATGGCTTCCGTCCACTCTTCGTTATGCTTATCTTATGTAGTTCCCCTCACGACTCAAATGAGACCAAACCTTGATATCATATCAATTGTGCAACAAGTCTCAAAATTCTCTCCCTAAGGCAATTTCACTGAATCATAAATATGATTCTCATATATGGCTTCCGTCCACTCTTCGTTATGCTTATCTTATGTAGTTTCATCCAAATGTTTTGACTCTCCTTCAAAATGCATCGAGTCAAAAAAATTTAGTTTCCTAACAGGCGAGTGATTCTTCAAAGAAATGTTAGTTTGCTCTCCTATAAAATAGCCAACAAAATTATGCATTTGCGGATTTCCTATAATAGGGAACACATCTCATTTGACTAGCTCAACCATGACTTTCTAATTCATGCAACACAATCTATTATTATATCGTAATAACACCTTATTAGTTGATTCCTTCCACTACACGAACGTCGTCTTAAATGCATATTACTTTAATCAGTGTCATGTAAATGGTAGAATATAATGTACCTTTAGGAACATATGTAGAACCATTCGTGAGTTTCCAATCATTCTATAAGAACAAGCTGTCATATTAACACATAGCTTAGTCCCTAAAAGCAAATTTGGTTGGATATATAAAACATACTTCACAAGCTATCACTAAAGAACTAAGAATGTCCCTTCTAAGCTTAACATGCAATGAAGGACCATTGTTGATAATCTAGTTGAATCTGTTATCAACAATATTAATAAAATGGGAGAAGGAAATCATTAGATGCTGCAATATCTTTGTCTTCAATAATCAATAGTAATGTCTTTTTTTTGCCCTTTATTACTGTGTTTCTTCTCCTTTTGAAAGTTGGCAGTACTTCTAAATGACTAAATATGTGATTTATTTACTACGATGGTGGCATTTCTACATTTTTGTTTTACTTATTTTTAGATTTACTTTTGACTTGGTCTCTGTCAGATGTCTTGAATTCCTGTCTTGCTTCTCTCTCGAGACTCAATAACCAATACCCTTTAGAGTGAAGAAGATTTTGCATGATTCCGATAGAAACTAGAAAACCGAAGTGAAATTGTGCATTAGTGACTCTGATAATGGATGAGCTTGAAGCTCTTTACAATGGTGGAAATATGGCAACAGAGTTCTAAGAACGCCAAGACCAGAGAGAGATCCTCTCATAAGTGAAGTGACGCGAACTAACTTTCTTCAACGATTCACTTACTAACCTTCCCTCTCTTTATAAAAGAAATTCTCCTAGACTTCAGGGTATATCCTAACAACCTTTATTCCTTCAGCTTCTTCTTCAATCTTCTTGAAACTGCACTAGTGTGAATTGGACCCGATGATGGGCCCCTCACATTTACTGGACCCTGATGCTCTATCAGTTTCCTCTCATCTCGTTAAAACACTCGTGACTTCTCATCTTGTTGAAAATACTTAGGAGTATATGGTTAGATGTTGATAAAATTTCTCATTCTTTACGTTAAGAAAATCTTCTGTTGTACGTAATTTGAATAATTTATATTTACCATTCAGTTTTCATTTGTTCCCATTGCCGCTAATTTGAATAATTAATCGTCATGGTTTGAAATTCGTATTTCTGAGTGATGTTGAATGTAGCTGTATGTATCCTTTGCCTATTTAGACTAATCATTCTGGTATAATCTTTCTGACAGGAAATTTCTGCTAACTCAAACGTAAAAGCATGA
Protein sequence

>MS.gene57468.t1

MATGTQAYGESWYWDNRYTNEPGPFDWYQKYITLAPIINLYVPKNQSILVVGSGNSAFSQGMVDEGGYKDVVNIDISSVVIDAMQKKYRDRPQLKYLKMDVRDMSAFASETFGSVIDKGTLDSILCGNNSRQHATKMLEEIWRVLKAKGVYILVTYGAPLYRLRLLRDSCSWTIKLHVIEKLASEEKSDNPLWELTKPIPLNNDGSSVDETLGLNPDVHYIYICTKEISANSNVKA