Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57587.t1 | XP_013458484.1 | 67.9 | 221 | 39 | 3 | 1 | 221 | 1 | 189 | 2.30E-41 | 179.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57587.t1 | I3SL07 | 67.9 | 221 | 39 | 3 | 1 | 221 | 1 | 189 | 1.6e-41 | 179.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051864 | MS.gene57587 | 0.91408 | 2.92E-84 | -1.69E-46 |
| MS.gene057717 | MS.gene57587 | -0.818643 | 1.76E-52 | -1.69E-46 |
| MS.gene058452 | MS.gene57587 | -0.804661 | 1.94E-49 | -1.69E-46 |
| MS.gene05926 | MS.gene57587 | -0.803346 | 3.64E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57587.t1 | MTR_4g126170 | 75.000 | 224 | 20 | 5 | 1 | 222 | 1 | 190 | 2.15e-95 | 276 |
| MS.gene57587.t1 | MTR_4g126210 | 49.442 | 269 | 59 | 11 | 5 | 221 | 1 | 244 | 1.52e-51 | 167 |
| MS.gene57587.t1 | MTR_2g039320 | 30.357 | 224 | 118 | 7 | 17 | 222 | 16 | 219 | 1.48e-13 | 67.8 |
| MS.gene57587.t1 | MTR_1g016100 | 48.276 | 58 | 30 | 0 | 163 | 220 | 242 | 299 | 6.15e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57587.t1 | AT2G26110 | 31.179 | 263 | 119 | 8 | 13 | 219 | 44 | 300 | 4.34e-20 | 87.0 |
Find 46 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGGACTCGGTCTCGGACTC+TGG | 0.364487 | 8.3:-4396197 | None:intergenic |
| ATCTTTGTTAACCTTGTCAT+TGG | 0.365409 | 8.3:+4396041 | MS.gene57587:CDS |
| AGTGTTCAATGACCTTTGAT+TGG | 0.386647 | 8.3:+4396423 | MS.gene57587:CDS |
| GACTTGACCGGTTCTGTAGA+TGG | 0.389512 | 8.3:-4396320 | None:intergenic |
| CCCCTCGCCATTGCAGTCGC+TGG | 0.396284 | 8.3:-4396485 | None:intergenic |
| ACTTGACCGGTTCTGTAGAT+GGG | 0.415711 | 8.3:-4396319 | None:intergenic |
| TCGGTCTCGGACTCTGGCTC+AGG | 0.437676 | 8.3:-4396191 | None:intergenic |
| GTGTTCAATGACCTTTGATT+GGG | 0.442400 | 8.3:+4396424 | MS.gene57587:CDS |
| GAAATTATTACTTGCTGCTA+TGG | 0.447524 | 8.3:-4395979 | None:intergenic |
| GTTTCAGATTCTGTTTGTGC+TGG | 0.457165 | 8.3:-4396296 | None:intergenic |
| TAACCAATGTTGGCTCGTTA+TGG | 0.483691 | 8.3:-4396163 | None:intergenic |
| AGTAGACTCGGACTCGGTCT+CGG | 0.491349 | 8.3:-4396204 | None:intergenic |
| ACATGTTTAAGAAGCAATTG+AGG | 0.499011 | 8.3:+4396570 | MS.gene57587:CDS |
| CTGAGATTGTGTGTTTGTTG+AGG | 0.500280 | 8.3:-4396125 | None:intergenic |
| TGAAACCCCATCTACAGAAC+CGG | 0.508418 | 8.3:+4396313 | MS.gene57587:CDS |
| TTCTTCTTCCTCCCAATCAA+AGG | 0.514922 | 8.3:-4396435 | None:intergenic |
| TGAGAATCACAATCGTGGTT+CGG | 0.515295 | 8.3:-4396347 | None:intergenic |
| GGAAGAAGAAGATGAAGAAA+CGG | 0.521334 | 8.3:+4396448 | MS.gene57587:CDS |
| TTCAATGACCTTTGATTGGG+AGG | 0.540849 | 8.3:+4396427 | MS.gene57587:CDS |
| TCAGGTTGTGTAACCAATGT+TGG | 0.542423 | 8.3:-4396173 | None:intergenic |
| ACAAAGATGAAAAGAGGAGA+AGG | 0.545362 | 8.3:-4396026 | None:intergenic |
| GCCAGCGACTGCAATGGCGA+GGG | 0.549742 | 8.3:+4396484 | MS.gene57587:CDS |
| GGCCAGCGACTGCAATGGCG+AGG | 0.557035 | 8.3:+4396483 | MS.gene57587:CDS |
| AAGGAGGCCAGCGACTGCAA+TGG | 0.559533 | 8.3:+4396478 | MS.gene57587:CDS |
| ATGAAGAAACGGTGGAGCGA+AGG | 0.562197 | 8.3:+4396459 | MS.gene57587:CDS |
| AAGAAACGGTGGAGCGAAGG+AGG | 0.565948 | 8.3:+4396462 | MS.gene57587:CDS |
| CTTGACCGGTTCTGTAGATG+GGG | 0.566562 | 8.3:-4396318 | None:intergenic |
| ATGTTGGCTCGTTATGGTGG+TGG | 0.574929 | 8.3:-4396157 | None:intergenic |
| TGGTTCGGATCCGACTTGAC+CGG | 0.581198 | 8.3:-4396332 | None:intergenic |
| AGGTTAACAAAGATGAAAAG+AGG | 0.582940 | 8.3:-4396032 | None:intergenic |
| AGAAGCAATTGAGGTTGCAG+AGG | 0.592934 | 8.3:+4396579 | MS.gene57587:CDS |
| CCAATGTTGGCTCGTTATGG+TGG | 0.593481 | 8.3:-4396160 | None:intergenic |
| CCAGCGACTGCAATGGCGAG+GGG | 0.593879 | 8.3:+4396485 | MS.gene57587:CDS |
| ATCTACAGAACCGGTCAAGT+CGG | 0.601074 | 8.3:+4396322 | MS.gene57587:CDS |
| GGGTTGAGTAGACTCGGACT+CGG | 0.601445 | 8.3:-4396210 | None:intergenic |
| CGATTGTGATTCTCATGATG+AGG | 0.604792 | 8.3:+4396355 | MS.gene57587:CDS |
| CCACCATAACGAGCCAACAT+TGG | 0.606919 | 8.3:+4396160 | MS.gene57587:CDS |
| GAGTTGGGGTTGAGTAGACT+CGG | 0.620169 | 8.3:-4396216 | None:intergenic |
| ATTTCATAGCCACATGCATG+TGG | 0.622525 | 8.3:+4395997 | MS.gene57587:CDS |
| AGAAGAAGATGAAGAAACGG+TGG | 0.628304 | 8.3:+4396451 | MS.gene57587:CDS |
| AGGTGTAAACCACATGCATG+TGG | 0.660037 | 8.3:-4396006 | None:intergenic |
| GAAGCGAGAAACGATAGCAA+TGG | 0.660890 | 8.3:-4396066 | None:intergenic |
| AAGACTCGCTGTAACAGTGA+AGG | 0.667221 | 8.3:-4396248 | None:intergenic |
| CATCATGAGAATCACAATCG+TGG | 0.680976 | 8.3:-4396352 | None:intergenic |
| TAGCAATGGTACCAATGACA+AGG | 0.697487 | 8.3:-4396052 | None:intergenic |
| TGAGGAGAATAGAAACAAAG+CGG | 0.717010 | 8.3:+4396373 | MS.gene57587:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACATGTTTAAGAAGCAATTG+AGG | + | chr8.3:4396570-4396589 | MS.gene57587:CDS | 30.0% | |
| AGGTTAACAAAGATGAAAAG+AGG | - | chr8.3:4396035-4396054 | None:intergenic | 30.0% | |
| ATCTTTGTTAACCTTGTCAT+TGG | + | chr8.3:4396041-4396060 | MS.gene57587:CDS | 30.0% | |
| ! | GAAATTATTACTTGCTGCTA+TGG | - | chr8.3:4395982-4396001 | None:intergenic | 30.0% |
| !!! | TTGTTGAGGTTGTTTTTGTT+GGG | - | chr8.3:4396114-4396133 | None:intergenic | 30.0% |
| !!! | TTTGTTGAGGTTGTTTTTGT+TGG | - | chr8.3:4396115-4396134 | None:intergenic | 30.0% |
| ACAAAGATGAAAAGAGGAGA+AGG | - | chr8.3:4396029-4396048 | None:intergenic | 35.0% | |
| AGTGTTCAATGACCTTTGAT+TGG | + | chr8.3:4396423-4396442 | MS.gene57587:CDS | 35.0% | |
| GGAAGAAGAAGATGAAGAAA+CGG | + | chr8.3:4396448-4396467 | MS.gene57587:CDS | 35.0% | |
| GTGTTCAATGACCTTTGATT+GGG | + | chr8.3:4396424-4396443 | MS.gene57587:CDS | 35.0% | |
| TGAGGAGAATAGAAACAAAG+CGG | + | chr8.3:4396373-4396392 | MS.gene57587:CDS | 35.0% | |
| !!! | AGGTTGTTTTTGTTGGGTTT+TGG | - | chr8.3:4396108-4396127 | None:intergenic | 35.0% |
| !!! | GGTTGTTTTTGTTGGGTTTT+GGG | - | chr8.3:4396107-4396126 | None:intergenic | 35.0% |
| !!! | TGAAGGTTTTAGAGTGAGTT+GGG | - | chr8.3:4396234-4396253 | None:intergenic | 35.0% |
| AGAAGAAGATGAAGAAACGG+TGG | + | chr8.3:4396451-4396470 | MS.gene57587:CDS | 40.0% | |
| ATTTCATAGCCACATGCATG+TGG | + | chr8.3:4395997-4396016 | MS.gene57587:CDS | 40.0% | |
| CATCATGAGAATCACAATCG+TGG | - | chr8.3:4396355-4396374 | None:intergenic | 40.0% | |
| CGATTGTGATTCTCATGATG+AGG | + | chr8.3:4396355-4396374 | MS.gene57587:CDS | 40.0% | |
| GTTTCAGATTCTGTTTGTGC+TGG | - | chr8.3:4396299-4396318 | None:intergenic | 40.0% | |
| TAGCAATGGTACCAATGACA+AGG | - | chr8.3:4396055-4396074 | None:intergenic | 40.0% | |
| TGAGAATCACAATCGTGGTT+CGG | - | chr8.3:4396350-4396369 | None:intergenic | 40.0% | |
| TTCAATGACCTTTGATTGGG+AGG | + | chr8.3:4396427-4396446 | MS.gene57587:CDS | 40.0% | |
| TTCTTCTTCCTCCCAATCAA+AGG | - | chr8.3:4396438-4396457 | None:intergenic | 40.0% | |
| ! | CTGAGATTGTGTGTTTGTTG+AGG | - | chr8.3:4396128-4396147 | None:intergenic | 40.0% |
| ! | TCAGGTTGTGTAACCAATGT+TGG | - | chr8.3:4396176-4396195 | None:intergenic | 40.0% |
| !! | TAACCAATGTTGGCTCGTTA+TGG | - | chr8.3:4396166-4396185 | None:intergenic | 40.0% |
| !!! | GAAGGTTTTAGAGTGAGTTG+GGG | - | chr8.3:4396233-4396252 | None:intergenic | 40.0% |
| !!! | GTGAAGGTTTTAGAGTGAGT+TGG | - | chr8.3:4396235-4396254 | None:intergenic | 40.0% |
| AAGACTCGCTGTAACAGTGA+AGG | - | chr8.3:4396251-4396270 | None:intergenic | 45.0% | |
| AGAAGCAATTGAGGTTGCAG+AGG | + | chr8.3:4396579-4396598 | MS.gene57587:CDS | 45.0% | |
| AGGTGTAAACCACATGCATG+TGG | - | chr8.3:4396009-4396028 | None:intergenic | 45.0% | |
| ATCTACAGAACCGGTCAAGT+CGG | + | chr8.3:4396322-4396341 | MS.gene57587:CDS | 45.0% | |
| GAAGCGAGAAACGATAGCAA+TGG | - | chr8.3:4396069-4396088 | None:intergenic | 45.0% | |
| TGAAACCCCATCTACAGAAC+CGG | + | chr8.3:4396313-4396332 | MS.gene57587:CDS | 45.0% | |
| ! | ACTTGACCGGTTCTGTAGAT+GGG | - | chr8.3:4396322-4396341 | None:intergenic | 45.0% |
| ATGAAGAAACGGTGGAGCGA+AGG | + | chr8.3:4396459-4396478 | MS.gene57587:CDS | 50.0% | |
| CCACCATAACGAGCCAACAT+TGG | + | chr8.3:4396160-4396179 | MS.gene57587:CDS | 50.0% | |
| GAGTTGGGGTTGAGTAGACT+CGG | - | chr8.3:4396219-4396238 | None:intergenic | 50.0% | |
| ! | CTTGACCGGTTCTGTAGATG+GGG | - | chr8.3:4396321-4396340 | None:intergenic | 50.0% |
| ! | GACTTGACCGGTTCTGTAGA+TGG | - | chr8.3:4396323-4396342 | None:intergenic | 50.0% |
| !! | ATGTTGGCTCGTTATGGTGG+TGG | - | chr8.3:4396160-4396179 | None:intergenic | 50.0% |
| !! | CCAATGTTGGCTCGTTATGG+TGG | - | chr8.3:4396163-4396182 | None:intergenic | 50.0% |
| AAGAAACGGTGGAGCGAAGG+AGG | + | chr8.3:4396462-4396481 | MS.gene57587:CDS | 55.0% | |
| GGGTTGAGTAGACTCGGACT+CGG | - | chr8.3:4396213-4396232 | None:intergenic | 55.0% | |
| ! | AGTAGACTCGGACTCGGTCT+CGG | - | chr8.3:4396207-4396226 | None:intergenic | 55.0% |
| ! | TGGTTCGGATCCGACTTGAC+CGG | - | chr8.3:4396335-4396354 | None:intergenic | 55.0% |
| AAGGAGGCCAGCGACTGCAA+TGG | + | chr8.3:4396478-4396497 | MS.gene57587:CDS | 60.0% | |
| CCAGCGACTGCAATGGCGAG+GGG | + | chr8.3:4396485-4396504 | MS.gene57587:CDS | 65.0% | |
| GCCAGCGACTGCAATGGCGA+GGG | + | chr8.3:4396484-4396503 | MS.gene57587:CDS | 65.0% | |
| ! | TCGGACTCGGTCTCGGACTC+TGG | - | chr8.3:4396200-4396219 | None:intergenic | 65.0% |
| ! | TCGGTCTCGGACTCTGGCTC+AGG | - | chr8.3:4396194-4396213 | None:intergenic | 65.0% |
| CCCCTCGCCATTGCAGTCGC+TGG | - | chr8.3:4396488-4396507 | None:intergenic | 70.0% | |
| GGCCAGCGACTGCAATGGCG+AGG | + | chr8.3:4396483-4396502 | MS.gene57587:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 4395969 | 4396637 | 4395969 | ID=MS.gene57587 |
| chr8.3 | mRNA | 4395969 | 4396637 | 4395969 | ID=MS.gene57587.t1;Parent=MS.gene57587 |
| chr8.3 | exon | 4395969 | 4396637 | 4395969 | ID=MS.gene57587.t1.exon1;Parent=MS.gene57587.t1 |
| chr8.3 | CDS | 4395969 | 4396637 | 4395969 | ID=cds.MS.gene57587.t1;Parent=MS.gene57587.t1 |
| Gene Sequence |
| Protein sequence |
