AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057717


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057717.t1 MTR_4g073950 98.947 190 2 0 1 190 3 192 5.77e-142 393
MS.gene057717.t1 MTR_4g013350 56.579 152 65 1 39 190 47 197 6.60e-61 188
MS.gene057717.t1 MTR_4g013330 54.248 153 69 1 39 190 47 199 4.02e-59 184
MS.gene057717.t1 MTR_4g013320 53.947 152 69 1 39 190 49 199 3.08e-57 179
MS.gene057717.t1 MTR_4g013345 53.947 152 69 1 39 190 46 196 6.43e-57 178
MS.gene057717.t1 MTR_4g013325 52.055 146 69 1 45 190 55 199 5.37e-54 171
MS.gene057717.t1 MTR_7g093820 47.692 195 96 4 1 190 1 194 9.05e-52 165
MS.gene057717.t1 MTR_7g093830 52.288 153 71 2 39 190 42 193 1.62e-51 164
MS.gene057717.t1 MTR_4g013335 49.342 152 74 1 39 190 48 196 3.59e-51 163
MS.gene057717.t1 MTR_7g093850 50.980 153 73 2 39 190 43 194 1.41e-50 162
MS.gene057717.t1 MTR_1g054525 50.658 152 72 2 39 190 44 192 1.78e-49 159
MS.gene057717.t1 MTR_7g070390 47.647 170 82 2 21 190 30 192 2.26e-49 159
MS.gene057717.t1 MTR_4g013385 44.444 153 83 1 38 190 40 190 1.80e-45 149
MS.gene057717.t1 MTR_0433s0040 44.444 153 83 1 38 190 40 190 1.80e-45 149
MS.gene057717.t1 MTR_7g093790 47.059 153 79 2 39 190 40 191 2.42e-45 148
MS.gene057717.t1 MTR_4g074020 78.313 83 18 0 1 83 1 83 1.94e-44 143
MS.gene057717.t1 MTR_3g105640 46.053 152 81 1 40 190 37 188 2.55e-44 145
MS.gene057717.t1 MTR_4g013310 47.020 151 77 2 40 190 50 197 3.10e-44 145
MS.gene057717.t1 MTR_7g093870 41.818 165 92 2 30 190 29 193 9.26e-43 142
MS.gene057717.t1 MTR_4g013770 42.105 152 88 0 39 190 40 191 1.12e-42 141
MS.gene057717.t1 MTR_1g056370 42.208 154 86 2 37 189 34 185 1.36e-42 141
MS.gene057717.t1 MTR_5g096120 45.033 151 78 3 44 190 40 189 7.03e-40 134
MS.gene057717.t1 MTR_3g105630 38.542 192 110 4 2 190 3 189 3.05e-38 130
MS.gene057717.t1 MTR_4g078885 38.667 150 89 1 41 190 39 185 5.37e-35 122
MS.gene057717.t1 MTR_3g034030 37.333 150 91 1 41 190 39 185 2.65e-33 117
MS.gene057717.t1 MTR_4g013355 40.260 154 79 3 38 190 38 179 1.12e-32 115
MS.gene057717.t1 MTR_4g122110 39.216 153 92 1 38 190 29 180 4.64e-30 108
MS.gene057717.t1 MTR_4g122130 37.580 157 96 2 34 190 26 180 1.42e-28 105
MS.gene057717.t1 MTR_4g049550 32.432 148 99 1 40 187 29 175 1.66e-18 79.0
MS.gene057717.t1 MTR_8g099115 32.624 141 87 3 36 169 29 168 4.80e-18 78.2
MS.gene057717.t1 MTR_4g049570 31.944 144 97 1 44 187 33 175 7.40e-18 77.4
MS.gene057717.t1 MTR_8g099135 29.240 171 97 4 6 169 11 164 2.68e-15 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057717.t1 AT2G21100 57.895 190 76 3 1 189 1 187 1.76e-79 235
MS.gene057717.t1 AT2G21100 57.895 190 76 3 1 189 1 187 1.76e-79 235
MS.gene057717.t1 AT1G58170 47.853 163 80 2 32 190 24 185 2.45e-50 161
MS.gene057717.t1 AT5G42510 52.258 155 71 2 35 189 31 182 1.11e-49 159
MS.gene057717.t1 AT5G49040 42.857 189 101 3 8 190 4 191 5.16e-48 155
MS.gene057717.t1 AT5G42500 50.649 154 73 2 36 189 35 185 2.38e-47 153
MS.gene057717.t1 AT1G55210 46.053 152 78 1 39 190 40 187 6.03e-47 152
MS.gene057717.t1 AT1G55210 46.053 152 78 1 39 190 40 187 6.03e-47 152
MS.gene057717.t1 AT3G13650 39.683 189 100 3 6 190 8 186 1.75e-46 151
MS.gene057717.t1 AT1G65870 46.452 155 82 1 35 189 36 189 2.07e-44 146
MS.gene057717.t1 AT1G22900 46.452 155 80 2 35 189 42 193 3.44e-43 143
MS.gene057717.t1 AT2G21110 46.452 155 80 1 39 190 32 186 1.83e-42 141
MS.gene057717.t1 AT3G13662 44.604 139 73 1 39 177 38 172 6.86e-40 134
MS.gene057717.t1 AT3G13660 49.565 115 58 0 76 190 11 125 1.64e-38 129
MS.gene057717.t1 AT5G49030 47.826 138 71 1 39 176 41 177 2.73e-37 137
MS.gene057717.t1 AT4G38700 39.744 156 89 3 40 190 35 190 7.48e-37 127
MS.gene057717.t1 AT3G58090 49.541 109 55 0 81 189 163 271 1.35e-30 113
MS.gene057717.t1 AT4G11210 36.458 96 60 1 78 173 72 166 1.07e-13 66.6
MS.gene057717.t1 AT4G11190 30.709 127 86 2 48 173 41 166 4.53e-11 59.3

Find 50 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGCCCACGAGCTTGGATTT+TGG 0.153982 4.4:+35920781 None:intergenic
GGATCAGATGTTTCAGTTAA+AGG 0.192736 4.4:+35920802 None:intergenic
TGAATTTAAAAGCAGGGAAT+TGG 0.267666 4.4:-35920990 MS.gene057717:CDS
TTTAAATTCAAGCTTTGAAT+TGG 0.322119 4.4:+35921003 None:intergenic
GGTAGCACATTTGCTCTTGT+TGG 0.341517 4.4:-35920670 MS.gene057717:CDS
GAACTGTGTAAGGTATGTTT+TGG 0.344125 4.4:+35920849 None:intergenic
ATGCCTGTTGTTGGAGGTAC+TGG 0.347518 4.4:-35920616 MS.gene057717:CDS
GAATTTAAAAGCAGGGAATT+GGG 0.356220 4.4:-35920989 MS.gene057717:CDS
ATGCCATTGCTAAGACTCAT+TGG 0.400986 4.4:-35920570 MS.gene057717:CDS
TTTGTGGATGGGCCGTATAA+TGG 0.409954 4.4:-35920691 MS.gene057717:CDS
GCAAATGTGCTACCATTATA+CGG 0.416384 4.4:+35920679 None:intergenic
TTGAGCTACTCCTTTGTGGA+TGG 0.418314 4.4:-35920703 MS.gene057717:CDS
TCATCGTGTCAGCAGGAGAT+TGG 0.421466 4.4:-35920739 MS.gene057717:CDS
GTCGTTGAGCTACTCCTTTG+TGG 0.422168 4.4:-35920707 MS.gene057717:CDS
TCAGCCACCTTGATCGCACT+TGG 0.429128 4.4:+35920886 None:intergenic
ACAACAGGCATCTCACGAAC+CGG 0.432988 4.4:+35920628 None:intergenic
AAATTCAAGCTTTGAATTGG+TGG 0.434298 4.4:+35921006 None:intergenic
TTGTGCTCGGCCCACGAGCT+TGG 0.436650 4.4:+35920774 None:intergenic
CATCGTGTCAGCAGGAGATT+GGG 0.452951 4.4:-35920738 MS.gene057717:CDS
GTTCGTGAGATGCCTGTTGT+TGG 0.459287 4.4:-35920625 MS.gene057717:CDS
CATTGGATTGATATTACTAC+TGG 0.460050 4.4:-35920553 MS.gene057717:CDS
TAATGTTTCCGAACTGTGTA+AGG 0.464436 4.4:+35920839 None:intergenic
ACTACTGGTGATGCTATTGT+TGG 0.469577 4.4:-35920538 MS.gene057717:CDS
TCACGAACCGGGTTCATAGC+AGG 0.470658 4.4:+35920640 None:intergenic
CAAAGCTTGAATTTAAAAGC+AGG 0.474536 4.4:-35920997 MS.gene057717:CDS
AAAACATACCTTACACAGTT+CGG 0.481108 4.4:-35920847 MS.gene057717:CDS
TGTCGTGGAAATAGAATTGA+AGG 0.486021 4.4:+35920926 None:intergenic
TGAGCTACTCCTTTGTGGAT+GGG 0.497718 4.4:-35920702 MS.gene057717:CDS
AGTCCAGTACCTCCAACAAC+AGG 0.500575 4.4:+35920613 None:intergenic
TTCAAGCTTTGAATTGGTGG+TGG 0.503760 4.4:+35921009 None:intergenic
TTACGCGTCATCGTGTCAGC+AGG 0.513526 4.4:-35920746 MS.gene057717:CDS
AAAGCTTGAATTTAAAAGCA+GGG 0.524166 4.4:-35920996 MS.gene057717:CDS
CGTGAGATGCCTGTTGTTGG+AGG 0.525744 4.4:-35920622 MS.gene057717:CDS
TGGCATAACCCCTTGCCATA+CGG 0.526929 4.4:+35920587 None:intergenic
TATTTCCACGACACACTAAG+TGG 0.531204 4.4:-35920916 MS.gene057717:CDS
CAGCCACCTTGATCGCACTT+GGG 0.549806 4.4:+35920887 None:intergenic
AAGCTCGTGGGCCGAGCACA+AGG 0.551866 4.4:-35920772 MS.gene057717:CDS
ACAGTTCGGAAACATTATGA+TGG 0.564400 4.4:-35920833 MS.gene057717:CDS
AATTTAAAAGCAGGGAATTG+GGG 0.569985 4.4:-35920988 MS.gene057717:CDS
CACGAACCGGGTTCATAGCA+GGG 0.575135 4.4:+35920641 None:intergenic
TGATCCAAAATCCAAGCTCG+TGG 0.590503 4.4:-35920785 MS.gene057717:CDS
CAACAGGCATCTCACGAACC+GGG 0.593590 4.4:+35920629 None:intergenic
ACGCGTAAATACCTTGTGCT+CGG 0.608058 4.4:+35920761 None:intergenic
AAAGAACCCTGCTATGAACC+CGG 0.622651 4.4:-35920647 MS.gene057717:CDS
GATCCAAAATCCAAGCTCGT+GGG 0.650480 4.4:-35920784 MS.gene057717:CDS
AATCCAATGAGTCTTAGCAA+TGG 0.659713 4.4:+35920567 None:intergenic
ATGCTATTAATAATGAACGA+GGG 0.668500 4.4:+35920504 None:intergenic
ATTATACGGCCCATCCACAA+AGG 0.683112 4.4:+35920693 None:intergenic
AAACCCAAGTGCGATCAAGG+TGG 0.716624 4.4:-35920890 MS.gene057717:CDS
AATGCTATTAATAATGAACG+AGG 0.719484 4.4:+35920503 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAAATTCAAGCTTTGAAT+TGG + chr4.4:35920570-35920589 None:intergenic 20.0%
! AAAGCTTGAATTTAAAAGCA+GGG - chr4.4:35920574-35920593 MS.gene057717:CDS 25.0%
AAAACATACCTTACACAGTT+CGG - chr4.4:35920723-35920742 MS.gene057717:CDS 30.0%
AATTTAAAAGCAGGGAATTG+GGG - chr4.4:35920582-35920601 MS.gene057717:CDS 30.0%
CAAAGCTTGAATTTAAAAGC+AGG - chr4.4:35920573-35920592 MS.gene057717:CDS 30.0%
CATTGGATTGATATTACTAC+TGG - chr4.4:35921017-35921036 MS.gene057717:CDS 30.0%
GAATTTAAAAGCAGGGAATT+GGG - chr4.4:35920581-35920600 MS.gene057717:CDS 30.0%
TGAATTTAAAAGCAGGGAAT+TGG - chr4.4:35920580-35920599 MS.gene057717:CDS 30.0%
!! AAATTCAAGCTTTGAATTGG+TGG + chr4.4:35920567-35920586 None:intergenic 30.0%
AATCCAATGAGTCTTAGCAA+TGG + chr4.4:35921006-35921025 None:intergenic 35.0%
ACAGTTCGGAAACATTATGA+TGG - chr4.4:35920737-35920756 MS.gene057717:CDS 35.0%
GCAAATGTGCTACCATTATA+CGG + chr4.4:35920894-35920913 None:intergenic 35.0%
GGATCAGATGTTTCAGTTAA+AGG + chr4.4:35920771-35920790 None:intergenic 35.0%
TAATGTTTCCGAACTGTGTA+AGG + chr4.4:35920734-35920753 None:intergenic 35.0%
TGTCGTGGAAATAGAATTGA+AGG + chr4.4:35920647-35920666 None:intergenic 35.0%
! GAACTGTGTAAGGTATGTTT+TGG + chr4.4:35920724-35920743 None:intergenic 35.0%
!!! TATGTTTTGGTCTTGTCTAC+AGG + chr4.4:35920711-35920730 None:intergenic 35.0%
AAAAAACCCAAGTGCGATCA+AGG - chr4.4:35920677-35920696 MS.gene057717:CDS 40.0%
ATGCCATTGCTAAGACTCAT+TGG - chr4.4:35921000-35921019 MS.gene057717:CDS 40.0%
TATTTCCACGACACACTAAG+TGG - chr4.4:35920654-35920673 MS.gene057717:CDS 40.0%
! ACTACTGGTGATGCTATTGT+TGG - chr4.4:35921032-35921051 MS.gene057717:CDS 40.0%
! TTTTTCCACTTAGTGTGTCG+TGG + chr4.4:35920662-35920681 None:intergenic 40.0%
!! TTCAAGCTTTGAATTGGTGG+TGG + chr4.4:35920564-35920583 None:intergenic 40.0%
AAAGAACCCTGCTATGAACC+CGG - chr4.4:35920923-35920942 MS.gene057717:CDS 45.0%
ACGCGTAAATACCTTGTGCT+CGG + chr4.4:35920812-35920831 None:intergenic 45.0%
ATTATACGGCCCATCCACAA+AGG + chr4.4:35920880-35920899 None:intergenic 45.0%
GATCCAAAATCCAAGCTCGT+GGG - chr4.4:35920786-35920805 MS.gene057717:CDS 45.0%
GGTAGCACATTTGCTCTTGT+TGG - chr4.4:35920900-35920919 MS.gene057717:CDS 45.0%
TGAGCTACTCCTTTGTGGAT+GGG - chr4.4:35920868-35920887 MS.gene057717:CDS 45.0%
TGATCCAAAATCCAAGCTCG+TGG - chr4.4:35920785-35920804 MS.gene057717:CDS 45.0%
TTGAGCTACTCCTTTGTGGA+TGG - chr4.4:35920867-35920886 MS.gene057717:CDS 45.0%
TTTGTGGATGGGCCGTATAA+TGG - chr4.4:35920879-35920898 MS.gene057717:CDS 45.0%
! TGGACTTTTCCGTATGGCAA+GGG - chr4.4:35920974-35920993 MS.gene057717:CDS 45.0%
!!! AGGTACTGGACTTTTCCGTA+TGG - chr4.4:35920968-35920987 MS.gene057717:CDS 45.0%
AAACCCAAGTGCGATCAAGG+TGG - chr4.4:35920680-35920699 MS.gene057717:CDS 50.0%
ACAACAGGCATCTCACGAAC+CGG + chr4.4:35920945-35920964 None:intergenic 50.0%
AGTCCAGTACCTCCAACAAC+AGG + chr4.4:35920960-35920979 None:intergenic 50.0%
CATCGTGTCAGCAGGAGATT+GGG - chr4.4:35920832-35920851 MS.gene057717:CDS 50.0%
GTCGTTGAGCTACTCCTTTG+TGG - chr4.4:35920863-35920882 MS.gene057717:CDS 50.0%
GTTCGTGAGATGCCTGTTGT+TGG - chr4.4:35920945-35920964 MS.gene057717:CDS 50.0%
TCATCGTGTCAGCAGGAGAT+TGG - chr4.4:35920831-35920850 MS.gene057717:CDS 50.0%
TGGCATAACCCCTTGCCATA+CGG + chr4.4:35920986-35921005 None:intergenic 50.0%
! ATGCCTGTTGTTGGAGGTAC+TGG - chr4.4:35920954-35920973 MS.gene057717:CDS 50.0%
! CTGGACTTTTCCGTATGGCA+AGG - chr4.4:35920973-35920992 MS.gene057717:CDS 50.0%
! GGACTTTTCCGTATGGCAAG+GGG - chr4.4:35920975-35920994 MS.gene057717:CDS 50.0%
CAACAGGCATCTCACGAACC+GGG + chr4.4:35920944-35920963 None:intergenic 55.0%
CACGAACCGGGTTCATAGCA+GGG + chr4.4:35920932-35920951 None:intergenic 55.0%
CAGCCACCTTGATCGCACTT+GGG + chr4.4:35920686-35920705 None:intergenic 55.0%
CGTGAGATGCCTGTTGTTGG+AGG - chr4.4:35920948-35920967 MS.gene057717:CDS 55.0%
TCACGAACCGGGTTCATAGC+AGG + chr4.4:35920933-35920952 None:intergenic 55.0%
TCAGCCACCTTGATCGCACT+TGG + chr4.4:35920687-35920706 None:intergenic 55.0%
TTACGCGTCATCGTGTCAGC+AGG - chr4.4:35920824-35920843 MS.gene057717:CDS 55.0%
! CGGCCCACGAGCTTGGATTT+TGG + chr4.4:35920792-35920811 None:intergenic 60.0%
AAGCTCGTGGGCCGAGCACA+AGG - chr4.4:35920798-35920817 MS.gene057717:CDS 65.0%
TTGTGCTCGGCCCACGAGCT+TGG + chr4.4:35920799-35920818 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 35920510 35921082 35920510 ID=MS.gene057717
chr4.4 mRNA 35920510 35921082 35920510 ID=MS.gene057717.t1;Parent=MS.gene057717
chr4.4 exon 35920510 35921082 35920510 ID=MS.gene057717.t1.exon1;Parent=MS.gene057717.t1
chr4.4 CDS 35920510 35921082 35920510 ID=cds.MS.gene057717.t1;Parent=MS.gene057717.t1
Gene Sequence

>MS.gene057717

ATGTCTAAGCAAAATGTGCTTGTGATGATACTTCTAATAAGTGCTATACTTCCACCACCAATTCAAAGCTTGAATTTAAAAGCAGGGAATTGGGGTGAAACTTTTCAAAACAAGAAACAAGAAACAGAAACAAACCTTCAATTCTATTTCCACGACACACTAAGTGGAAAAAACCCAAGTGCGATCAAGGTGGCTGAACCTGTAGACAAGACCAAAACATACCTTACACAGTTCGGAAACATTATGATGGCAGATGATCCTTTAACTGAAACATCTGATCCAAAATCCAAGCTCGTGGGCCGAGCACAAGGTATTTACGCGTCATCGTGTCAGCAGGAGATTGGGCTTCTTATGTCGTTGAGCTACTCCTTTGTGGATGGGCCGTATAATGGTAGCACATTTGCTCTTGTTGGAAAGAACCCTGCTATGAACCCGGTTCGTGAGATGCCTGTTGTTGGAGGTACTGGACTTTTCCGTATGGCAAGGGGTTATGCCATTGCTAAGACTCATTGGATTGATATTACTACTGGTGATGCTATTGTTGGCTACAATGTCACCCTCGTTCATTATTAA

Protein sequence

>MS.gene057717.t1

MSKQNVLVMILLISAILPPPIQSLNLKAGNWGETFQNKKQETETNLQFYFHDTLSGKNPSAIKVAEPVDKTKTYLTQFGNIMMADDPLTETSDPKSKLVGRAQGIYASSCQQEIGLLMSLSYSFVDGPYNGSTFALVGKNPAMNPVREMPVVGGTGLFRMARGYAIAKTHWIDITTGDAIVGYNVTLVHY