Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57588.t1 | XP_013458484.1 | 74 | 123 | 9 | 1 | 1 | 123 | 90 | 189 | 1.30E-14 | 89.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57588.t1 | I3SL07 | 74.0 | 123 | 9 | 1 | 1 | 123 | 90 | 189 | 9.5e-15 | 89.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051439 | MS.gene57588 | -0.804919 | 1.71E-49 | -1.69E-46 |
| MS.gene051864 | MS.gene57588 | 0.902113 | 1.35E-78 | -1.69E-46 |
| MS.gene056697 | MS.gene57588 | -0.801501 | 8.74E-49 | -1.69E-46 |
| MS.gene057717 | MS.gene57588 | -0.825212 | 5.30E-54 | -1.69E-46 |
| MS.gene058452 | MS.gene57588 | -0.819878 | 9.22E-53 | -1.69E-46 |
| MS.gene05926 | MS.gene57588 | -0.810342 | 1.21E-50 | -1.69E-46 |
| MS.gene060405 | MS.gene57588 | -0.819907 | 9.08E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57588.t1 | MTR_4g126170 | 70.968 | 124 | 13 | 2 | 1 | 124 | 90 | 190 | 8.38e-40 | 131 |
| MS.gene57588.t1 | MTR_4g126210 | 63.415 | 123 | 24 | 4 | 1 | 123 | 143 | 244 | 6.46e-28 | 102 |
| MS.gene57588.t1 | MTR_1g016100 | 48.276 | 58 | 30 | 0 | 65 | 122 | 242 | 299 | 3.10e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57588.t1 | AT2G26110 | 56.897 | 58 | 24 | 1 | 64 | 121 | 244 | 300 | 8.27e-12 | 60.8 |
Find 26 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGTTCAATGACCTTTGAT+TGG | 0.386647 | 8.4:-8731424 | MS.gene57588:CDS |
| GACTTGACCGGTTCTGTAGA+TGG | 0.389512 | 8.4:+8731527 | None:intergenic |
| CCCCTCGCCATTGCAGTCGC+TGG | 0.396284 | 8.4:+8731362 | None:intergenic |
| ACTTGACCGGTTCTGTAGAT+GGG | 0.415711 | 8.4:+8731528 | None:intergenic |
| GTGTTCAATGACCTTTGATT+GGG | 0.442400 | 8.4:-8731423 | MS.gene57588:CDS |
| GTTTCAGATTCTGTTTGTGC+TGG | 0.457165 | 8.4:+8731551 | None:intergenic |
| ACATGTTTAAGAAGCAATTG+AGG | 0.499011 | 8.4:-8731277 | MS.gene57588:CDS |
| TGAAACCCCATCTACAGAAC+CGG | 0.508418 | 8.4:-8731534 | MS.gene57588:CDS |
| TTCTTCTTCCTCCCAATCAA+AGG | 0.514922 | 8.4:+8731412 | None:intergenic |
| TGAGAATCACAATCGTGGTT+CGG | 0.515295 | 8.4:+8731500 | None:intergenic |
| GGAAGAAGAAGATGAAGAAA+CGG | 0.521334 | 8.4:-8731399 | MS.gene57588:CDS |
| TTCAATGACCTTTGATTGGG+AGG | 0.540849 | 8.4:-8731420 | MS.gene57588:CDS |
| GCCAGCGACTGCAATGGCGA+GGG | 0.549742 | 8.4:-8731363 | MS.gene57588:CDS |
| GGCCAGCGACTGCAATGGCG+AGG | 0.557035 | 8.4:-8731364 | MS.gene57588:CDS |
| AAGGAGGCCAGCGACTGCAA+TGG | 0.559533 | 8.4:-8731369 | MS.gene57588:CDS |
| ATGAAGAAACGGTGGAGCGA+AGG | 0.562197 | 8.4:-8731388 | MS.gene57588:CDS |
| AAGAAACGGTGGAGCGAAGG+AGG | 0.565948 | 8.4:-8731385 | MS.gene57588:CDS |
| CTTGACCGGTTCTGTAGATG+GGG | 0.566562 | 8.4:+8731529 | None:intergenic |
| TGGTTCGGATCCGACTTGAC+CGG | 0.581198 | 8.4:+8731515 | None:intergenic |
| AGAAGCAATTGAGGTTGCAG+AGG | 0.592934 | 8.4:-8731268 | MS.gene57588:CDS |
| CCAGCGACTGCAATGGCGAG+GGG | 0.593879 | 8.4:-8731362 | MS.gene57588:CDS |
| ATCTACAGAACCGGTCAAGT+CGG | 0.601074 | 8.4:-8731525 | MS.gene57588:CDS |
| CGATTGTGATTCTCATGATG+AGG | 0.604792 | 8.4:-8731492 | MS.gene57588:CDS |
| AGAAGAAGATGAAGAAACGG+TGG | 0.628304 | 8.4:-8731396 | MS.gene57588:CDS |
| CATCATGAGAATCACAATCG+TGG | 0.680976 | 8.4:+8731495 | None:intergenic |
| TGAGGAGAATAGAAACAAAG+CGG | 0.717010 | 8.4:-8731474 | MS.gene57588:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACATGTTTAAGAAGCAATTG+AGG | - | chr8.4:8731539-8731558 | MS.gene57588:CDS | 30.0% | |
| AGTGTTCAATGACCTTTGAT+TGG | - | chr8.4:8731392-8731411 | MS.gene57588:CDS | 35.0% | |
| GGAAGAAGAAGATGAAGAAA+CGG | - | chr8.4:8731417-8731436 | MS.gene57588:CDS | 35.0% | |
| GTGTTCAATGACCTTTGATT+GGG | - | chr8.4:8731393-8731412 | MS.gene57588:CDS | 35.0% | |
| TGAGGAGAATAGAAACAAAG+CGG | - | chr8.4:8731342-8731361 | MS.gene57588:CDS | 35.0% | |
| AGAAGAAGATGAAGAAACGG+TGG | - | chr8.4:8731420-8731439 | MS.gene57588:CDS | 40.0% | |
| CATCATGAGAATCACAATCG+TGG | + | chr8.4:8731324-8731343 | None:intergenic | 40.0% | |
| CGATTGTGATTCTCATGATG+AGG | - | chr8.4:8731324-8731343 | MS.gene57588:CDS | 40.0% | |
| GTTTCAGATTCTGTTTGTGC+TGG | + | chr8.4:8731268-8731287 | None:intergenic | 40.0% | |
| TGAGAATCACAATCGTGGTT+CGG | + | chr8.4:8731319-8731338 | None:intergenic | 40.0% | |
| TTCAATGACCTTTGATTGGG+AGG | - | chr8.4:8731396-8731415 | MS.gene57588:CDS | 40.0% | |
| TTCTTCTTCCTCCCAATCAA+AGG | + | chr8.4:8731407-8731426 | None:intergenic | 40.0% | |
| AGAAGCAATTGAGGTTGCAG+AGG | - | chr8.4:8731548-8731567 | MS.gene57588:CDS | 45.0% | |
| ATCTACAGAACCGGTCAAGT+CGG | - | chr8.4:8731291-8731310 | MS.gene57588:CDS | 45.0% | |
| TGAAACCCCATCTACAGAAC+CGG | - | chr8.4:8731282-8731301 | MS.gene57588:CDS | 45.0% | |
| ! | ACTTGACCGGTTCTGTAGAT+GGG | + | chr8.4:8731291-8731310 | None:intergenic | 45.0% |
| ATGAAGAAACGGTGGAGCGA+AGG | - | chr8.4:8731428-8731447 | MS.gene57588:CDS | 50.0% | |
| ! | CTTGACCGGTTCTGTAGATG+GGG | + | chr8.4:8731290-8731309 | None:intergenic | 50.0% |
| ! | GACTTGACCGGTTCTGTAGA+TGG | + | chr8.4:8731292-8731311 | None:intergenic | 50.0% |
| AAGAAACGGTGGAGCGAAGG+AGG | - | chr8.4:8731431-8731450 | MS.gene57588:CDS | 55.0% | |
| ! | TGGTTCGGATCCGACTTGAC+CGG | + | chr8.4:8731304-8731323 | None:intergenic | 55.0% |
| AAGGAGGCCAGCGACTGCAA+TGG | - | chr8.4:8731447-8731466 | MS.gene57588:CDS | 60.0% | |
| CCAGCGACTGCAATGGCGAG+GGG | - | chr8.4:8731454-8731473 | MS.gene57588:CDS | 65.0% | |
| GCCAGCGACTGCAATGGCGA+GGG | - | chr8.4:8731453-8731472 | MS.gene57588:CDS | 65.0% | |
| CCCCTCGCCATTGCAGTCGC+TGG | + | chr8.4:8731457-8731476 | None:intergenic | 70.0% | |
| GGCCAGCGACTGCAATGGCG+AGG | - | chr8.4:8731452-8731471 | MS.gene57588:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 8731232 | 8731606 | 8731232 | ID=MS.gene57588 |
| chr8.4 | mRNA | 8731232 | 8731606 | 8731232 | ID=MS.gene57588.t1;Parent=MS.gene57588 |
| chr8.4 | exon | 8731232 | 8731606 | 8731232 | ID=MS.gene57588.t1.exon1;Parent=MS.gene57588.t1 |
| chr8.4 | CDS | 8731232 | 8731606 | 8731232 | ID=cds.MS.gene57588.t1;Parent=MS.gene57588.t1 |
| Gene Sequence |
| Protein sequence |
