Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57590.t1 | XP_013458484.1 | 67.9 | 221 | 39 | 3 | 1 | 221 | 1 | 189 | 2.30E-41 | 179.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57590.t1 | I3SL07 | 67.9 | 221 | 39 | 3 | 1 | 221 | 1 | 189 | 1.6e-41 | 179.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051864 | MS.gene57590 | 0.891716 | 3.08E-74 | -1.69E-46 |
| MS.gene057717 | MS.gene57590 | -0.807783 | 4.27E-50 | -1.69E-46 |
| MS.gene058452 | MS.gene57590 | -0.80366 | 3.13E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57590.t1 | MTR_4g126170 | 75.000 | 224 | 20 | 5 | 1 | 222 | 1 | 190 | 2.15e-95 | 276 |
| MS.gene57590.t1 | MTR_4g126210 | 49.442 | 269 | 59 | 11 | 5 | 221 | 1 | 244 | 1.52e-51 | 167 |
| MS.gene57590.t1 | MTR_2g039320 | 30.357 | 224 | 118 | 7 | 17 | 222 | 16 | 219 | 1.48e-13 | 67.8 |
| MS.gene57590.t1 | MTR_1g016100 | 48.276 | 58 | 30 | 0 | 163 | 220 | 242 | 299 | 6.15e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57590.t1 | AT2G26110 | 31.179 | 263 | 119 | 8 | 13 | 219 | 44 | 300 | 4.34e-20 | 87.0 |
Find 46 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGGACTCGGTCTCGGACTC+TGG | 0.364487 | 8.4:-8632374 | None:intergenic |
| ATCTTTGTTAACCTTGTCAT+TGG | 0.365409 | 8.4:+8632218 | MS.gene57590:CDS |
| AGTGTTCAATGACCTTTGAT+TGG | 0.386647 | 8.4:+8632600 | MS.gene57590:CDS |
| GACTTGACCGGTTCTGTAGA+TGG | 0.389512 | 8.4:-8632497 | None:intergenic |
| CCCCTCGCCATTGCAGTCGC+TGG | 0.396284 | 8.4:-8632662 | None:intergenic |
| ACTTGACCGGTTCTGTAGAT+GGG | 0.415711 | 8.4:-8632496 | None:intergenic |
| TCGGTCTCGGACTCTGGCTC+AGG | 0.437676 | 8.4:-8632368 | None:intergenic |
| GTGTTCAATGACCTTTGATT+GGG | 0.442400 | 8.4:+8632601 | MS.gene57590:CDS |
| GAAATTATTACTTGCTGCTA+TGG | 0.447524 | 8.4:-8632156 | None:intergenic |
| GTTTCAGATTCTGTTTGTGC+TGG | 0.457165 | 8.4:-8632473 | None:intergenic |
| TAACCAATGTTGGCTCGTTA+TGG | 0.483691 | 8.4:-8632340 | None:intergenic |
| AGTAGACTCGGACTCGGTCT+CGG | 0.491349 | 8.4:-8632381 | None:intergenic |
| ACATGTTTAAGAAGCAATTG+AGG | 0.499011 | 8.4:+8632747 | MS.gene57590:CDS |
| CTGAGATTGTGTGTTTGTTG+AGG | 0.500280 | 8.4:-8632302 | None:intergenic |
| TGAAACCCCATCTACAGAAC+CGG | 0.508418 | 8.4:+8632490 | MS.gene57590:CDS |
| TTCTTCTTCCTCCCAATCAA+AGG | 0.514922 | 8.4:-8632612 | None:intergenic |
| TGAGAATCACAATCGTGGTT+CGG | 0.515295 | 8.4:-8632524 | None:intergenic |
| GGAAGAAGAAGATGAAGAAA+CGG | 0.521334 | 8.4:+8632625 | MS.gene57590:CDS |
| TTCAATGACCTTTGATTGGG+AGG | 0.540849 | 8.4:+8632604 | MS.gene57590:CDS |
| TCAGGTTGTGTAACCAATGT+TGG | 0.542423 | 8.4:-8632350 | None:intergenic |
| ACAAAGATGAAAAGAGGAGA+AGG | 0.545362 | 8.4:-8632203 | None:intergenic |
| GCCAGCGACTGCAATGGCGA+GGG | 0.549742 | 8.4:+8632661 | MS.gene57590:CDS |
| GGCCAGCGACTGCAATGGCG+AGG | 0.557035 | 8.4:+8632660 | MS.gene57590:CDS |
| AAGGAGGCCAGCGACTGCAA+TGG | 0.559533 | 8.4:+8632655 | MS.gene57590:CDS |
| ATGAAGAAACGGTGGAGCGA+AGG | 0.562197 | 8.4:+8632636 | MS.gene57590:CDS |
| AAGAAACGGTGGAGCGAAGG+AGG | 0.565948 | 8.4:+8632639 | MS.gene57590:CDS |
| CTTGACCGGTTCTGTAGATG+GGG | 0.566562 | 8.4:-8632495 | None:intergenic |
| ATGTTGGCTCGTTATGGTGG+TGG | 0.574929 | 8.4:-8632334 | None:intergenic |
| TGGTTCGGATCCGACTTGAC+CGG | 0.581198 | 8.4:-8632509 | None:intergenic |
| AGGTTAACAAAGATGAAAAG+AGG | 0.582940 | 8.4:-8632209 | None:intergenic |
| AGAAGCAATTGAGGTTGCAG+AGG | 0.592934 | 8.4:+8632756 | MS.gene57590:CDS |
| CCAATGTTGGCTCGTTATGG+TGG | 0.593481 | 8.4:-8632337 | None:intergenic |
| CCAGCGACTGCAATGGCGAG+GGG | 0.593879 | 8.4:+8632662 | MS.gene57590:CDS |
| ATCTACAGAACCGGTCAAGT+CGG | 0.601074 | 8.4:+8632499 | MS.gene57590:CDS |
| GGGTTGAGTAGACTCGGACT+CGG | 0.601445 | 8.4:-8632387 | None:intergenic |
| CGATTGTGATTCTCATGATG+AGG | 0.604792 | 8.4:+8632532 | MS.gene57590:CDS |
| CCACCATAACGAGCCAACAT+TGG | 0.606919 | 8.4:+8632337 | MS.gene57590:CDS |
| GAGTTGGGGTTGAGTAGACT+CGG | 0.620169 | 8.4:-8632393 | None:intergenic |
| ATTTCATAGCCACATGCATG+TGG | 0.622525 | 8.4:+8632174 | MS.gene57590:CDS |
| AGAAGAAGATGAAGAAACGG+TGG | 0.628304 | 8.4:+8632628 | MS.gene57590:CDS |
| AGGTGTAAACCACATGCATG+TGG | 0.660037 | 8.4:-8632183 | None:intergenic |
| GAAGCGAGAAACGATAGCAA+TGG | 0.660890 | 8.4:-8632243 | None:intergenic |
| AAGACTCGCTGTAACAGTGA+AGG | 0.667221 | 8.4:-8632425 | None:intergenic |
| CATCATGAGAATCACAATCG+TGG | 0.680976 | 8.4:-8632529 | None:intergenic |
| TAGCAATGGTACCAATGACA+AGG | 0.697487 | 8.4:-8632229 | None:intergenic |
| TGAGGAGAATAGAAACAAAG+CGG | 0.717010 | 8.4:+8632550 | MS.gene57590:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACATGTTTAAGAAGCAATTG+AGG | + | chr8.4:8632747-8632766 | MS.gene57590:CDS | 30.0% | |
| AGGTTAACAAAGATGAAAAG+AGG | - | chr8.4:8632212-8632231 | None:intergenic | 30.0% | |
| ATCTTTGTTAACCTTGTCAT+TGG | + | chr8.4:8632218-8632237 | MS.gene57590:CDS | 30.0% | |
| ! | GAAATTATTACTTGCTGCTA+TGG | - | chr8.4:8632159-8632178 | None:intergenic | 30.0% |
| !!! | TTGTTGAGGTTGTTTTTGTT+GGG | - | chr8.4:8632291-8632310 | None:intergenic | 30.0% |
| !!! | TTTGTTGAGGTTGTTTTTGT+TGG | - | chr8.4:8632292-8632311 | None:intergenic | 30.0% |
| ACAAAGATGAAAAGAGGAGA+AGG | - | chr8.4:8632206-8632225 | None:intergenic | 35.0% | |
| AGTGTTCAATGACCTTTGAT+TGG | + | chr8.4:8632600-8632619 | MS.gene57590:CDS | 35.0% | |
| GGAAGAAGAAGATGAAGAAA+CGG | + | chr8.4:8632625-8632644 | MS.gene57590:CDS | 35.0% | |
| GTGTTCAATGACCTTTGATT+GGG | + | chr8.4:8632601-8632620 | MS.gene57590:CDS | 35.0% | |
| TGAGGAGAATAGAAACAAAG+CGG | + | chr8.4:8632550-8632569 | MS.gene57590:CDS | 35.0% | |
| !!! | AGGTTGTTTTTGTTGGGTTT+TGG | - | chr8.4:8632285-8632304 | None:intergenic | 35.0% |
| !!! | GGTTGTTTTTGTTGGGTTTT+GGG | - | chr8.4:8632284-8632303 | None:intergenic | 35.0% |
| !!! | TGAAGGTTTTAGAGTGAGTT+GGG | - | chr8.4:8632411-8632430 | None:intergenic | 35.0% |
| AGAAGAAGATGAAGAAACGG+TGG | + | chr8.4:8632628-8632647 | MS.gene57590:CDS | 40.0% | |
| ATTTCATAGCCACATGCATG+TGG | + | chr8.4:8632174-8632193 | MS.gene57590:CDS | 40.0% | |
| CATCATGAGAATCACAATCG+TGG | - | chr8.4:8632532-8632551 | None:intergenic | 40.0% | |
| CGATTGTGATTCTCATGATG+AGG | + | chr8.4:8632532-8632551 | MS.gene57590:CDS | 40.0% | |
| GTTTCAGATTCTGTTTGTGC+TGG | - | chr8.4:8632476-8632495 | None:intergenic | 40.0% | |
| TAGCAATGGTACCAATGACA+AGG | - | chr8.4:8632232-8632251 | None:intergenic | 40.0% | |
| TGAGAATCACAATCGTGGTT+CGG | - | chr8.4:8632527-8632546 | None:intergenic | 40.0% | |
| TTCAATGACCTTTGATTGGG+AGG | + | chr8.4:8632604-8632623 | MS.gene57590:CDS | 40.0% | |
| TTCTTCTTCCTCCCAATCAA+AGG | - | chr8.4:8632615-8632634 | None:intergenic | 40.0% | |
| ! | CTGAGATTGTGTGTTTGTTG+AGG | - | chr8.4:8632305-8632324 | None:intergenic | 40.0% |
| ! | TCAGGTTGTGTAACCAATGT+TGG | - | chr8.4:8632353-8632372 | None:intergenic | 40.0% |
| !! | TAACCAATGTTGGCTCGTTA+TGG | - | chr8.4:8632343-8632362 | None:intergenic | 40.0% |
| !!! | GAAGGTTTTAGAGTGAGTTG+GGG | - | chr8.4:8632410-8632429 | None:intergenic | 40.0% |
| !!! | GTGAAGGTTTTAGAGTGAGT+TGG | - | chr8.4:8632412-8632431 | None:intergenic | 40.0% |
| AAGACTCGCTGTAACAGTGA+AGG | - | chr8.4:8632428-8632447 | None:intergenic | 45.0% | |
| AGAAGCAATTGAGGTTGCAG+AGG | + | chr8.4:8632756-8632775 | MS.gene57590:CDS | 45.0% | |
| AGGTGTAAACCACATGCATG+TGG | - | chr8.4:8632186-8632205 | None:intergenic | 45.0% | |
| ATCTACAGAACCGGTCAAGT+CGG | + | chr8.4:8632499-8632518 | MS.gene57590:CDS | 45.0% | |
| GAAGCGAGAAACGATAGCAA+TGG | - | chr8.4:8632246-8632265 | None:intergenic | 45.0% | |
| TGAAACCCCATCTACAGAAC+CGG | + | chr8.4:8632490-8632509 | MS.gene57590:CDS | 45.0% | |
| ! | ACTTGACCGGTTCTGTAGAT+GGG | - | chr8.4:8632499-8632518 | None:intergenic | 45.0% |
| ATGAAGAAACGGTGGAGCGA+AGG | + | chr8.4:8632636-8632655 | MS.gene57590:CDS | 50.0% | |
| CCACCATAACGAGCCAACAT+TGG | + | chr8.4:8632337-8632356 | MS.gene57590:CDS | 50.0% | |
| GAGTTGGGGTTGAGTAGACT+CGG | - | chr8.4:8632396-8632415 | None:intergenic | 50.0% | |
| ! | CTTGACCGGTTCTGTAGATG+GGG | - | chr8.4:8632498-8632517 | None:intergenic | 50.0% |
| ! | GACTTGACCGGTTCTGTAGA+TGG | - | chr8.4:8632500-8632519 | None:intergenic | 50.0% |
| !! | ATGTTGGCTCGTTATGGTGG+TGG | - | chr8.4:8632337-8632356 | None:intergenic | 50.0% |
| !! | CCAATGTTGGCTCGTTATGG+TGG | - | chr8.4:8632340-8632359 | None:intergenic | 50.0% |
| AAGAAACGGTGGAGCGAAGG+AGG | + | chr8.4:8632639-8632658 | MS.gene57590:CDS | 55.0% | |
| GGGTTGAGTAGACTCGGACT+CGG | - | chr8.4:8632390-8632409 | None:intergenic | 55.0% | |
| ! | AGTAGACTCGGACTCGGTCT+CGG | - | chr8.4:8632384-8632403 | None:intergenic | 55.0% |
| ! | TGGTTCGGATCCGACTTGAC+CGG | - | chr8.4:8632512-8632531 | None:intergenic | 55.0% |
| AAGGAGGCCAGCGACTGCAA+TGG | + | chr8.4:8632655-8632674 | MS.gene57590:CDS | 60.0% | |
| CCAGCGACTGCAATGGCGAG+GGG | + | chr8.4:8632662-8632681 | MS.gene57590:CDS | 65.0% | |
| GCCAGCGACTGCAATGGCGA+GGG | + | chr8.4:8632661-8632680 | MS.gene57590:CDS | 65.0% | |
| ! | TCGGACTCGGTCTCGGACTC+TGG | - | chr8.4:8632377-8632396 | None:intergenic | 65.0% |
| ! | TCGGTCTCGGACTCTGGCTC+AGG | - | chr8.4:8632371-8632390 | None:intergenic | 65.0% |
| CCCCTCGCCATTGCAGTCGC+TGG | - | chr8.4:8632665-8632684 | None:intergenic | 70.0% | |
| GGCCAGCGACTGCAATGGCG+AGG | + | chr8.4:8632660-8632679 | MS.gene57590:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 8632146 | 8632814 | 8632146 | ID=MS.gene57590 |
| chr8.4 | mRNA | 8632146 | 8632814 | 8632146 | ID=MS.gene57590.t1;Parent=MS.gene57590 |
| chr8.4 | exon | 8632146 | 8632814 | 8632146 | ID=MS.gene57590.t1.exon1;Parent=MS.gene57590.t1 |
| chr8.4 | CDS | 8632146 | 8632814 | 8632146 | ID=cds.MS.gene57590.t1;Parent=MS.gene57590.t1 |
| Gene Sequence |
| Protein sequence |
