Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57832.t1 | RHN52554.1 | 85.1 | 436 | 51 | 3 | 1 | 431 | 1 | 427 | 2.90E-178 | 634.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57832.t1 | O81242 | 48.1 | 376 | 161 | 6 | 1 | 363 | 1 | 355 | 8.8e-75 | 282.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57832.t1 | A0A396HKZ0 | 85.1 | 436 | 51 | 3 | 1 | 431 | 1 | 427 | 2.1e-178 | 634.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05157 | MS.gene57832 | 0.803366 | 3.61E-49 | -1.69E-46 |
| MS.gene05215 | MS.gene57832 | 0.822593 | 2.18E-53 | -1.69E-46 |
| MS.gene052665 | MS.gene57832 | 0.815654 | 8.27E-52 | -1.69E-46 |
| MS.gene05604 | MS.gene57832 | 0.805691 | 1.18E-49 | -1.69E-46 |
| MS.gene057446 | MS.gene57832 | 0.804848 | 1.77E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene57832 | 0.814036 | 1.89E-51 | -1.69E-46 |
| MS.gene058010 | MS.gene57832 | 0.816766 | 4.67E-52 | -1.69E-46 |
| MS.gene060803 | MS.gene57832 | 0.800244 | 1.58E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57832.t1 | MTR_6g077860 | 90.138 | 436 | 26 | 3 | 1 | 431 | 1 | 424 | 0.0 | 730 |
| MS.gene57832.t1 | MTR_6g077860 | 93.017 | 401 | 12 | 2 | 1 | 396 | 1 | 390 | 0.0 | 707 |
| MS.gene57832.t1 | MTR_6g077860 | 92.768 | 401 | 12 | 3 | 1 | 396 | 1 | 389 | 0.0 | 701 |
| MS.gene57832.t1 | MTR_6g077860 | 92.437 | 357 | 11 | 2 | 1 | 352 | 1 | 346 | 0.0 | 625 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57832.t1 | AT1G48380 | 50.986 | 355 | 141 | 7 | 22 | 363 | 21 | 355 | 4.05e-99 | 300 |
| MS.gene57832.t1 | AT1G48380 | 46.891 | 386 | 141 | 8 | 22 | 363 | 21 | 386 | 1.86e-92 | 284 |
Find 81 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTGCGTTCAATGGTTTC+TGG | 0.002739 | 6.1:-68977043 | None:intergenic |
| AAATCCAGTTTGGCTTCTTC+TGG | 0.128617 | 6.1:-68979800 | None:intergenic |
| CAGGGTCGAATGAAGTTATT+TGG | 0.165215 | 6.1:+68979279 | MS.gene57832:intron |
| AAATATCCTTATAGGCATTT+TGG | 0.171132 | 6.1:+68980774 | MS.gene57832:CDS |
| AACCAGCAGGCTGCGACTTC+TGG | 0.220258 | 6.1:-68982459 | None:intergenic |
| CAAACTGAACATGACTTTAA+AGG | 0.229485 | 6.1:+68979944 | MS.gene57832:CDS |
| CCTGGTTTGTTTGCTCCTAT+TGG | 0.244363 | 6.1:+68977220 | MS.gene57832:CDS |
| GATTGGTGATCTTAAGGATT+TGG | 0.256759 | 6.1:+68977252 | MS.gene57832:CDS |
| ATTTCTTCTGGTGATGATTC+TGG | 0.283304 | 6.1:+68980673 | MS.gene57832:CDS |
| AGAGTCAAGTATCTATTCTT+TGG | 0.286324 | 6.1:-68979315 | None:intergenic |
| ATGAATTGAACTTCTAGTTT+TGG | 0.292180 | 6.1:-68977107 | None:intergenic |
| ACCAGCTCAAATATCCTTAT+AGG | 0.296418 | 6.1:+68980766 | MS.gene57832:CDS |
| CATCACACTCTTTCCACCTT+TGG | 0.302640 | 6.1:-68979346 | None:intergenic |
| TCCATGGGGAGTTGATCTTT+TGG | 0.326043 | 6.1:-68981116 | None:intergenic |
| ATTGGTGATCTTAAGGATTT+GGG | 0.333826 | 6.1:+68977253 | MS.gene57832:CDS |
| TGGGAAGATTGGTGATCTTA+AGG | 0.343649 | 6.1:+68977246 | MS.gene57832:CDS |
| CTTAAAAGACACAACAGAAT+TGG | 0.355540 | 6.1:+68980087 | MS.gene57832:CDS |
| AAACTGAACATGACTTTAAA+GGG | 0.359722 | 6.1:+68979945 | MS.gene57832:CDS |
| AGATTTGCTGAAATTTCTTC+TGG | 0.361470 | 6.1:+68980661 | MS.gene57832:intron |
| TCTTGCGTTCAATGGTTTCT+GGG | 0.363161 | 6.1:-68977042 | None:intergenic |
| TTTCGGATGCATGGTGGATT+GGG | 0.364946 | 6.1:+68979762 | MS.gene57832:CDS |
| TTTAGGATTCTGACGAAGAT+TGG | 0.368574 | 6.1:+68985683 | MS.gene57832:intron |
| AATCCAGTTTGGCTTCTTCT+GGG | 0.379974 | 6.1:-68979799 | None:intergenic |
| TGATGGAGATTTATTTGAAT+TGG | 0.386109 | 6.1:-68982327 | None:intergenic |
| GGGATGCCAAAATGCCTATA+AGG | 0.391636 | 6.1:-68980780 | None:intergenic |
| TTAGTCCCACATCGGATAGA+TGG | 0.411943 | 6.1:-68983767 | None:intergenic |
| GAACCCAGAAGAAGCCAAAC+TGG | 0.414656 | 6.1:+68979796 | MS.gene57832:CDS |
| TTCCTTGGGAAAATCCAGTT+TGG | 0.417867 | 6.1:-68979810 | None:intergenic |
| GTCTCTGGTGACTCTGGCTC+TGG | 0.422830 | 6.1:-68980028 | None:intergenic |
| ATCCTCATTATTCAAGAAAG+TGG | 0.428728 | 6.1:+68981199 | MS.gene57832:CDS |
| GACTTTAAAGGGGCGGGTGC+AGG | 0.430535 | 6.1:+68979956 | MS.gene57832:CDS |
| CTTACCTCGTACAGTTCCTT+GGG | 0.432334 | 6.1:-68979824 | None:intergenic |
| GTTCTCGCTGACTGACGAAC+TGG | 0.437627 | 6.1:-68980112 | None:intergenic |
| ACTTACCTCGTACAGTTCCT+TGG | 0.439596 | 6.1:-68979825 | None:intergenic |
| TTAGGATTCTGACGAAGATT+GGG | 0.441138 | 6.1:+68985684 | MS.gene57832:intron |
| TTCTTCATGTTCAGAAAGGT+CGG | 0.441717 | 6.1:-68980707 | None:intergenic |
| GGCCAGAAGTCGCAGCCTGC+TGG | 0.442610 | 6.1:+68982457 | MS.gene57832:CDS |
| TCCATTGTTGCTAAACATCA+TGG | 0.444208 | 6.1:+68977142 | MS.gene57832:CDS |
| TTTGAGTCTCTTGCGTTCAA+TGG | 0.445478 | 6.1:-68977050 | None:intergenic |
| AGAGGTGTCTCTGGTGACTC+TGG | 0.456583 | 6.1:-68980034 | None:intergenic |
| TCATCTTCAAGAGGTGTCTC+TGG | 0.457203 | 6.1:-68980043 | None:intergenic |
| CCAATCTTCCCACCACCAAT+AGG | 0.459539 | 6.1:-68977235 | None:intergenic |
| CACGTTGTTTGAAAATGCTA+AGG | 0.461867 | 6.1:-68977074 | None:intergenic |
| AGGGTCGAATGAAGTTATTT+GGG | 0.462249 | 6.1:+68979280 | MS.gene57832:intron |
| CCTATTGGTGGTGGGAAGAT+TGG | 0.462694 | 6.1:+68977235 | MS.gene57832:CDS |
| TTGTTTGCTCCTATTGGTGG+TGG | 0.464358 | 6.1:+68977226 | MS.gene57832:CDS |
| TTCCACTTTCTTGAATAATG+AGG | 0.470268 | 6.1:-68981201 | None:intergenic |
| TCTTCATGTTCAGAAAGGTC+GGG | 0.475296 | 6.1:-68980706 | None:intergenic |
| GGTTTGTTTGCTCCTATTGG+TGG | 0.477434 | 6.1:+68977223 | MS.gene57832:CDS |
| GGTTTCTGGGTTTGTTGCGT+CGG | 0.484306 | 6.1:-68977029 | None:intergenic |
| ATTCAAGAAAGTGGAAGTAA+AGG | 0.493741 | 6.1:+68981208 | MS.gene57832:CDS |
| GGGAAATCGAGGTAAAGAAC+AGG | 0.495137 | 6.1:-68977286 | None:intergenic |
| AATAAATCTCCATCATCAAA+AGG | 0.500680 | 6.1:+68982335 | MS.gene57832:CDS |
| AGGATGAAGGACCCAAGAAA+AGG | 0.505436 | 6.1:+68982605 | MS.gene57832:intron |
| TCTTTACCTCGATTTCCCTC+AGG | 0.514638 | 6.1:+68977291 | MS.gene57832:CDS |
| GGATGAAGGACCCAAGAAAA+GGG | 0.517110 | 6.1:+68982606 | MS.gene57832:intron |
| TGTCTGTTGTTGCAGGATGA+AGG | 0.518087 | 6.1:+68982592 | MS.gene57832:intron |
| GCCTATAAGGATATTTGAGC+TGG | 0.523542 | 6.1:-68980767 | None:intergenic |
| CTTCATGTTCAGAAAGGTCG+GGG | 0.526407 | 6.1:-68980705 | None:intergenic |
| TGTTTAGCAACAATGGAAGA+AGG | 0.545180 | 6.1:-68977136 | None:intergenic |
| GCAGCTTCTGTAGTTAATCA+TGG | 0.545893 | 6.1:+68979980 | MS.gene57832:CDS |
| TAGTCCCACATCGGATAGAT+GGG | 0.546135 | 6.1:-68983766 | None:intergenic |
| TGTTTGCTCCTATTGGTGGT+GGG | 0.547345 | 6.1:+68977227 | MS.gene57832:CDS |
| ACCATGATGTTTAGCAACAA+TGG | 0.563611 | 6.1:-68977143 | None:intergenic |
| ACAAACCAGGAAACGAGAAA+AGG | 0.564488 | 6.1:-68977207 | None:intergenic |
| CTTGAGAAGAGTTTATAAAG+AGG | 0.568537 | 6.1:-68983740 | None:intergenic |
| CCAATAGGAGCAAACAAACC+AGG | 0.575919 | 6.1:-68977220 | None:intergenic |
| GAACATGACTTTAAAGGGGC+GGG | 0.578239 | 6.1:+68979950 | MS.gene57832:CDS |
| ACTCTACAGTTCTCCAAAGG+TGG | 0.579477 | 6.1:+68979333 | MS.gene57832:CDS |
| GAGAAGAGTTTATAAAGAGG+TGG | 0.586116 | 6.1:-68983737 | None:intergenic |
| TCTGATAAATCATCTTCAAG+AGG | 0.593258 | 6.1:-68980052 | None:intergenic |
| GAAAAGGGCAAGAAAGACCA+AGG | 0.609681 | 6.1:+68982621 | MS.gene57832:CDS |
| TGAACATGACTTTAAAGGGG+CGG | 0.636157 | 6.1:+68979949 | MS.gene57832:CDS |
| TCCAAAAGATCAACTCCCCA+TGG | 0.637769 | 6.1:+68981115 | MS.gene57832:CDS |
| GAACAACCTGAGGGAAATCG+AGG | 0.649397 | 6.1:-68977297 | None:intergenic |
| TTGACTCTACAGTTCTCCAA+AGG | 0.655390 | 6.1:+68979330 | MS.gene57832:CDS |
| TGGAAAGAGTGTGATGTGTG+AGG | 0.674756 | 6.1:+68979353 | MS.gene57832:CDS |
| AACTGAACATGACTTTAAAG+GGG | 0.685862 | 6.1:+68979946 | MS.gene57832:CDS |
| AGTTATTGACATTGACGATG+AGG | 0.704093 | 6.1:+68981085 | MS.gene57832:CDS |
| GGAAATCGAGGTAAAGAACA+GGG | 0.704855 | 6.1:-68977285 | None:intergenic |
| ATATTATTAAGAAATCTCAG+AGG | 0.780918 | 6.1:+68977170 | MS.gene57832:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 68976983 | 68985714 | 68976983 | ID=MS.gene57832 |
| chr6.1 | mRNA | 68976983 | 68985714 | 68976983 | ID=MS.gene57832.t1;Parent=MS.gene57832 |
| chr6.1 | exon | 68976983 | 68977312 | 68976983 | ID=MS.gene57832.t1.exon1;Parent=MS.gene57832.t1 |
| chr6.1 | CDS | 68976983 | 68977312 | 68976983 | ID=cds.MS.gene57832.t1;Parent=MS.gene57832.t1 |
| chr6.1 | exon | 68979282 | 68979392 | 68979282 | ID=MS.gene57832.t1.exon2;Parent=MS.gene57832.t1 |
| chr6.1 | CDS | 68979282 | 68979392 | 68979282 | ID=cds.MS.gene57832.t1;Parent=MS.gene57832.t1 |
| chr6.1 | exon | 68979755 | 68979841 | 68979755 | ID=MS.gene57832.t1.exon3;Parent=MS.gene57832.t1 |
| chr6.1 | CDS | 68979755 | 68979841 | 68979755 | ID=cds.MS.gene57832.t1;Parent=MS.gene57832.t1 |
| chr6.1 | exon | 68979938 | 68980152 | 68979938 | ID=MS.gene57832.t1.exon4;Parent=MS.gene57832.t1 |
| chr6.1 | CDS | 68979938 | 68980152 | 68979938 | ID=cds.MS.gene57832.t1;Parent=MS.gene57832.t1 |
| chr6.1 | exon | 68980663 | 68980803 | 68980663 | ID=MS.gene57832.t1.exon5;Parent=MS.gene57832.t1 |
| chr6.1 | CDS | 68980663 | 68980803 | 68980663 | ID=cds.MS.gene57832.t1;Parent=MS.gene57832.t1 |
| chr6.1 | exon | 68981067 | 68981229 | 68981067 | ID=MS.gene57832.t1.exon6;Parent=MS.gene57832.t1 |
| chr6.1 | CDS | 68981067 | 68981229 | 68981067 | ID=cds.MS.gene57832.t1;Parent=MS.gene57832.t1 |
| chr6.1 | exon | 68982317 | 68982356 | 68982317 | ID=MS.gene57832.t1.exon7;Parent=MS.gene57832.t1 |
| chr6.1 | CDS | 68982317 | 68982356 | 68982317 | ID=cds.MS.gene57832.t1;Parent=MS.gene57832.t1 |
| chr6.1 | exon | 68982452 | 68982501 | 68982452 | ID=MS.gene57832.t1.exon8;Parent=MS.gene57832.t1 |
| chr6.1 | CDS | 68982452 | 68982501 | 68982452 | ID=cds.MS.gene57832.t1;Parent=MS.gene57832.t1 |
| chr6.1 | exon | 68982607 | 68982655 | 68982607 | ID=MS.gene57832.t1.exon9;Parent=MS.gene57832.t1 |
| chr6.1 | CDS | 68982607 | 68982655 | 68982607 | ID=cds.MS.gene57832.t1;Parent=MS.gene57832.t1 |
| chr6.1 | exon | 68983124 | 68983158 | 68983124 | ID=MS.gene57832.t1.exon10;Parent=MS.gene57832.t1 |
| chr6.1 | CDS | 68983124 | 68983158 | 68983124 | ID=cds.MS.gene57832.t1;Parent=MS.gene57832.t1 |
| chr6.1 | exon | 68983732 | 68983779 | 68983732 | ID=MS.gene57832.t1.exon11;Parent=MS.gene57832.t1 |
| chr6.1 | CDS | 68983732 | 68983779 | 68983732 | ID=cds.MS.gene57832.t1;Parent=MS.gene57832.t1 |
| chr6.1 | exon | 68985688 | 68985714 | 68985688 | ID=MS.gene57832.t1.exon12;Parent=MS.gene57832.t1 |
| chr6.1 | CDS | 68985688 | 68985714 | 68985688 | ID=cds.MS.gene57832.t1;Parent=MS.gene57832.t1 |
| Gene Sequence |
| Protein sequence |
