AlfalfaGEDB | MS.gene58519

M. sativa cultivar XinJiangDaYe / MS.gene58519

KEGG: K03671
GO: GO:0003674 GO:0003824 GO:0006662 GO:0008150 GO:0008152 GO:0009987 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0018904 GO:0019725 GO:0042592 GO:0044281 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008 GO:0071704
PFAM: PF00085.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58519.t1	XP_003613544.1	99.2	126	1	0	1	126	1	126	1.10E-64	255.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58519.t1	Q38879	48.6	111	57	0	15	125	22	132	3.3e-30	132.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58519.t1	D8L5E0	99.2	126	1	0	1	126	1	126	7.8e-65	255.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055909	MS.gene58519	0.800795	1.22E-48	-1.69E-46
MS.gene057609	MS.gene58519	0.910052	2.88E-82	-1.69E-46
MS.gene057610	MS.gene58519	0.927702	8.12E-92	-1.69E-46
MS.gene058075	MS.gene58519	0.817794	2.74E-52	-1.69E-46
MS.gene059674	MS.gene58519	0.817726	2.84E-52	-1.69E-46
MS.gene059807	MS.gene58519	0.805492	1.30E-49	-1.69E-46
MS.gene059876	MS.gene58519	0.804969	1.67E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58519.t1	MTR_5g037890	99.206	126	1	0	1	126	1	126	7.07e-93	264
MS.gene58519.t1	MTR_5g037950	67.460	126	40	1	1	125	1	126	9.77e-53	162
MS.gene58519.t1	MTR_5g037930	67.188	128	40	1	1	126	1	128	1.74e-52	162
MS.gene58519.t1	MTR_7g009070	56.604	106	46	0	20	125	28	133	5.22e-44	140
MS.gene58519.t1	MTR_3g112410	46.847	111	59	0	14	124	2	112	2.26e-36	120
MS.gene58519.t1	MTR_1g023140	42.478	113	65	0	14	126	2	114	1.59e-34	116
MS.gene58519.t1	MTR_5g021180	44.144	111	62	0	14	124	4	114	2.20e-34	115
MS.gene58519.t1	MTR_8g107460	42.308	104	60	0	18	121	254	357	7.49e-25	97.1
MS.gene58519.t1	MTR_8g005320	34.286	105	69	0	17	121	25	129	1.74e-23	88.6
MS.gene58519.t1	MTR_7g073680	36.190	105	67	0	17	121	23	127	2.71e-23	88.2
MS.gene58519.t1	MTR_2g010750	39.048	105	64	0	18	122	9	113	4.64e-23	87.4
MS.gene58519.t1	MTR_8g006685	34.286	105	69	0	17	121	94	198	3.04e-22	87.4
MS.gene58519.t1	MTR_8g006560	34.286	105	69	0	17	121	94	198	3.04e-22	87.4
MS.gene58519.t1	MTR_4g081380	37.383	107	63	2	18	122	10	114	5.90e-21	82.0
MS.gene58519.t1	MTR_2g082590	32.381	105	71	0	18	122	10	114	7.40e-20	79.0
MS.gene58519.t1	MTR_2g082590	33.628	113	66	2	10	122	24	127	1.01e-19	79.0
MS.gene58519.t1	MTR_7g080250	37.500	88	54	1	40	126	94	181	2.71e-15	68.6
MS.gene58519.t1	MTR_1g098660	32.558	86	55	2	39	123	77	160	2.80e-15	68.6
MS.gene58519.t1	MTR_4g094690	34.146	82	52	1	44	123	85	166	1.55e-14	66.6
MS.gene58519.t1	MTR_8g059015	34.091	88	56	2	38	123	87	174	5.52e-14	65.1
MS.gene58519.t1	MTR_3g089065	31.313	99	63	3	27	123	76	171	1.04e-13	64.3
MS.gene58519.t1	MTR_2g079420	34.091	88	56	2	38	123	84	171	3.69e-13	62.8
MS.gene58519.t1	MTR_1g114290	32.911	79	49	2	38	115	84	159	1.44e-12	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58519.t1	AT5G39950	48.649	111	57	0	15	125	22	132	6.24e-42	135
MS.gene58519.t1	AT1G59730	42.188	128	71	1	1	125	1	128	2.31e-35	118
MS.gene58519.t1	AT1G69880	47.664	107	56	0	17	123	36	142	3.67e-35	119
MS.gene58519.t1	AT3G51030	42.727	110	63	0	14	123	2	111	2.00e-33	113
MS.gene58519.t1	AT1G19730	46.429	112	59	1	14	124	2	113	9.90e-33	111
MS.gene58519.t1	AT1G45145	43.925	107	60	0	18	124	5	111	1.81e-31	108
MS.gene58519.t1	AT5G42980	44.037	109	61	0	18	126	5	113	5.28e-31	107
MS.gene58519.t1	AT3G17880	40.777	103	61	0	19	121	264	366	7.58e-23	91.7
MS.gene58519.t1	AT3G17880	40.777	103	61	0	19	121	271	373	8.74e-23	91.7
MS.gene58519.t1	AT3G56420	38.043	92	57	0	28	119	50	141	2.90e-21	83.6
MS.gene58519.t1	AT3G56420	38.043	92	57	0	28	119	50	141	2.90e-21	83.6
MS.gene58519.t1	AT3G56420	38.043	92	57	0	28	119	50	141	2.90e-21	83.6
MS.gene58519.t1	AT3G08710	33.333	111	72	1	11	121	18	126	1.06e-20	81.6
MS.gene58519.t1	AT3G08710	33.333	111	72	1	11	121	18	126	1.06e-20	81.6
MS.gene58519.t1	AT3G08710	33.333	111	72	1	11	121	18	126	1.06e-20	81.6
MS.gene58519.t1	AT1G11530	34.286	105	69	0	18	122	2	106	4.55e-19	77.0
MS.gene58519.t1	AT2G40790	34.783	92	60	0	28	119	50	141	3.40e-17	73.2
MS.gene58519.t1	AT2G40790	34.783	92	60	0	28	119	50	141	3.40e-17	73.2
MS.gene58519.t1	AT5G16400	38.636	88	53	1	40	126	97	184	1.63e-16	72.0
MS.gene58519.t1	AT3G02730	40.230	87	51	1	41	126	88	174	3.29e-16	71.2
MS.gene58519.t1	AT1G43560	29.060	117	78	3	1	112	33	149	3.80e-16	70.5
MS.gene58519.t1	AT4G03520	40.845	71	41	1	43	112	101	171	4.20e-15	68.2
MS.gene58519.t1	AT1G03680	38.272	81	48	2	45	123	97	177	1.87e-14	66.6
MS.gene58519.t1	AT1G76760	29.474	95	60	3	33	120	70	164	2.35e-14	66.2
MS.gene58519.t1	AT3G15360	32.967	91	53	3	38	123	102	189	2.43e-14	66.6
MS.gene58519.t1	AT2G35010	37.349	83	48	2	44	123	110	191	1.72e-13	64.3
MS.gene58519.t1	AT2G35010	37.349	83	48	2	44	123	110	191	1.72e-13	64.3
MS.gene58519.t1	AT1G31020	30.000	110	75	1	16	123	47	156	1.78e-13	63.5
MS.gene58519.t1	AT1G50320	36.709	79	46	2	38	115	85	160	1.01e-12	62.0
MS.gene58519.t1	AT1G52990	32.967	91	59	2	34	123	222	311	7.04e-12	61.2

Find 24 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATTCCATTCCACTGCTAAA+TGG	0.238150	5.3:-26031564	MS.gene58519:CDS
GCAGCAAAATCTTTCATAAC+TGG	0.263663	5.3:+26031356	None:intergenic
GCTTGATGTTGATGAGTTAA+TGG	0.271195	5.3:-26031306	MS.gene58519:intron
TTATTGATTTCACAGCTAAA+TGG	0.322109	5.3:-26031403	MS.gene58519:CDS
GGGAGTGTCTCAAACCTTCC+AGG	0.392325	5.3:-26031177	MS.gene58519:CDS
TAAAGTGGTGGGAGCAAGAA+AGG	0.432390	5.3:-26031105	MS.gene58519:CDS
TTCTTAATGAGTAGAAAAGT+TGG	0.438267	5.3:+26031143	None:intergenic
GTGTGGACCTTGCAAAGTTA+TGG	0.487071	5.3:-26031381	MS.gene58519:CDS
AGGAAAGGTTGTTGATAAAG+TGG	0.493130	5.3:-26031120	MS.gene58519:CDS
TAGCAGTGGAATGGAATGTA+AGG	0.498154	5.3:+26031569	None:intergenic
TCTACTCATTAAGAAAGGAA+AGG	0.517021	5.3:-26031135	MS.gene58519:CDS
ATAAACAAAATTATACAAGT+TGG	0.527315	5.3:+26031621	None:intergenic
CATTCCATTTAGCAGTGGAA+TGG	0.530623	5.3:+26031560	None:intergenic
TGGAATGGAATGTAAGGACA+TGG	0.568326	5.3:+26031575	None:intergenic
AAGTTGGCAATGAATGCACC+TGG	0.579245	5.3:+26031159	None:intergenic
TGGCAATGAATGCACCTGGA+AGG	0.589602	5.3:+26031163	None:intergenic
GATTTCACAGCTAAATGGTG+TGG	0.590907	5.3:-26031398	MS.gene58519:CDS
AAGGTTGTTGATAAAGTGGT+GGG	0.601867	5.3:-26031116	MS.gene58519:CDS
ACTGGATCCATAACTTTGCA+AGG	0.602265	5.3:+26031374	None:intergenic
ATGAGCATTCCATTTAGCAG+TGG	0.609708	5.3:+26031555	None:intergenic
AAAGGTTGTTGATAAAGTGG+TGG	0.610487	5.3:-26031117	MS.gene58519:CDS
CAAGAAAGGAGGAACTACAA+AGG	0.696570	5.3:-26031091	MS.gene58519:CDS
AGTGGTGGGAGCAAGAAAGG+AGG	0.703427	5.3:-26031102	MS.gene58519:CDS
GGAATGGAATGTAAGGACAT+GGG	0.736550	5.3:+26031576	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAAACAAAATTATACAAGT+TGG	+	chr5.3:26031077-26031096	None:intergenic	15.0%
!!!	TTTTAAAGAAACTAATAAAC+TGG	-	chr5.3:26031171-26031190	MS.gene58519:CDS	15.0%
!!!	TGTTTTTTCTTTTGTATAGA+TGG	-	chr5.3:26031269-26031288	MS.gene58519:intron	20.0%
!	AAACTAATAAACTGGTATGT+TGG	-	chr5.3:26031179-26031198	MS.gene58519:CDS	25.0%
!	TAACAAACTAGTTAAAGTGA+AGG	+	chr5.3:26031430-26031449	None:intergenic	25.0%
!	TTCTTAATGAGTAGAAAAGT+TGG	+	chr5.3:26031555-26031574	None:intergenic	25.0%
!!	ACTTTTCTACTCATTAAGAA+AGG	-	chr5.3:26031555-26031574	MS.gene58519:CDS	25.0%
!!	TGTTTTTCCCACAATATAAT+TGG	-	chr5.3:26031490-26031509	MS.gene58519:intron	25.0%
!!!	TTATTGATTTCACAGCTAAA+TGG	-	chr5.3:26031292-26031311	MS.gene58519:intron	25.0%
	TCTACTCATTAAGAAAGGAA+AGG	-	chr5.3:26031560-26031579	MS.gene58519:CDS	30.0%
	CCACAATATAATTGGCAACA+GGG	-	chr5.3:26031498-26031517	MS.gene58519:intron	35.0%
	CCTGTTGCCAATTATATTGT+GGG	+	chr5.3:26031500-26031519	None:intergenic	35.0%
	GCAGCAAAATCTTTCATAAC+TGG	+	chr5.3:26031342-26031361	None:intergenic	35.0%
!	AAAGGTTGTTGATAAAGTGG+TGG	-	chr5.3:26031578-26031597	MS.gene58519:CDS	35.0%
!	AAGGTTGTTGATAAAGTGGT+GGG	-	chr5.3:26031579-26031598	MS.gene58519:CDS	35.0%
!	AGGAAAGGTTGTTGATAAAG+TGG	-	chr5.3:26031575-26031594	MS.gene58519:CDS	35.0%
!	GCTTGATGTTGATGAGTTAA+TGG	-	chr5.3:26031389-26031408	MS.gene58519:CDS	35.0%
	ACAAATCAGTTCTCAAGTGC+TGG	+	chr5.3:26031473-26031492	None:intergenic	40.0%
	ACTGGATCCATAACTTTGCA+AGG	+	chr5.3:26031324-26031343	None:intergenic	40.0%
	ATGAGCATTCCATTTAGCAG+TGG	+	chr5.3:26031143-26031162	None:intergenic	40.0%
	CAAGAAAGGAGGAACTACAA+AGG	-	chr5.3:26031604-26031623	MS.gene58519:CDS	40.0%
	CATTCCATTCCACTGCTAAA+TGG	-	chr5.3:26031131-26031150	MS.gene58519:CDS	40.0%
	CATTCCATTTAGCAGTGGAA+TGG	+	chr5.3:26031138-26031157	None:intergenic	40.0%
	CCCACAATATAATTGGCAAC+AGG	-	chr5.3:26031497-26031516	MS.gene58519:intron	40.0%
	CCCTGTTGCCAATTATATTG+TGG	+	chr5.3:26031501-26031520	None:intergenic	40.0%
	GATTTCACAGCTAAATGGTG+TGG	-	chr5.3:26031297-26031316	MS.gene58519:intron	40.0%
	GGAATGGAATGTAAGGACAT+GGG	+	chr5.3:26031122-26031141	None:intergenic	40.0%
	TAGCAGTGGAATGGAATGTA+AGG	+	chr5.3:26031129-26031148	None:intergenic	40.0%
	TGGAATGGAATGTAAGGACA+TGG	+	chr5.3:26031123-26031142	None:intergenic	40.0%
	AAGTTGGCAATGAATGCACC+TGG	+	chr5.3:26031539-26031558	None:intergenic	45.0%
	GTGTGGACCTTGCAAAGTTA+TGG	-	chr5.3:26031314-26031333	MS.gene58519:CDS	45.0%
!	TAAAGTGGTGGGAGCAAGAA+AGG	-	chr5.3:26031590-26031609	MS.gene58519:CDS	45.0%
	TGGCAATGAATGCACCTGGA+AGG	+	chr5.3:26031535-26031554	None:intergenic	50.0%
!	AGTGGTGGGAGCAAGAAAGG+AGG	-	chr5.3:26031593-26031612	MS.gene58519:CDS	55.0%
!!	GGGAGTGTCTCAAACCTTCC+AGG	-	chr5.3:26031518-26031537	MS.gene58519:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	26031067	26031650	26031067	ID=MS.gene58519
chr5.3	mRNA	26031067	26031650	26031067	ID=MS.gene58519.t1;Parent=MS.gene58519
chr5.3	exon	26031525	26031650	26031525	ID=MS.gene58519.t1.exon1;Parent=MS.gene58519.t1
chr5.3	CDS	26031525	26031650	26031525	ID=cds.MS.gene58519.t1;Parent=MS.gene58519.t1
chr5.3	exon	26031307	26031429	26031307	ID=MS.gene58519.t1.exon2;Parent=MS.gene58519.t1
chr5.3	CDS	26031307	26031429	26031307	ID=cds.MS.gene58519.t1;Parent=MS.gene58519.t1
chr5.3	exon	26031067	26031198	26031067	ID=MS.gene58519.t1.exon3;Parent=MS.gene58519.t1
chr5.3	CDS	26031067	26031198	26031067	ID=cds.MS.gene58519.t1;Parent=MS.gene58519.t1

Gene Sequence

>MS.gene58519

ATGAGTTCCAACTTGTATAATTTTGTTTATGTTGATAGATCAACAGAGTCATCCCATGTCCTTACATTCCATTCCACTGCTAAATGGAATGCTCATTTTGAAGCTTTTAAAGAAACTAATAAACTGGTATGTTGGTGTAGATAAATTCTTTAACTAGCTATAGTATCTTAATAGATAAAATTTAGTTTTCTGATGTAATTAGTGTTTTTTCTTTTGTATAGATGGTTATTGATTTCACAGCTAAATGGTGTGGACCTTGCAAAGTTATGGATCCAGTTATGAAAGATTTTGCTGCAAAATACACTGATGTTGAGTTCATTAAGCTTGATGTTGATGAGTTAATGGTATGTTACAAATTATCCTTCACTTTAACTAGTTTGTTATTTAATTTATAACATGATTTCCAGCACTTGAGAACTGATTTGTTTTTCCCACAATATAATTGGCAACAGGGAGTGTCTCAAACCTTCCAGGTGCATTCATTGCCAACTTTTCTACTCATTAAGAAAGGAAAGGTTGTTGATAAAGTGGTGGGAGCAAGAAAGGAGGAACTACAAAGGATGATTGAAAAACAAAGAAATTAA

Protein sequence

>MS.gene58519.t1

MSSNLYNFVYVDRSTESSHVLTFHSTAKWNAHFEAFKETNKLMVIDFTAKWCGPCKVMDPVMKDFAAKYTDVEFIKLDVDELMGVSQTFQVHSLPTFLLIKKGKVVDKVVGARKEELQRMIEKQRN