AlfalfaGEDB | MS.gene057609

M. sativa cultivar XinJiangDaYe / MS.gene057609

KEGG: K03671
GO: GO:0003674 GO:0003824 GO:0006662 GO:0008150 GO:0008152 GO:0009987 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0018904 GO:0019725 GO:0042592 GO:0044281 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008 GO:0071704
PFAM: PF00085.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057609.t1	XP_003613544.1	95.2	126	6	0	1	126	1	126	1.30E-62	248.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057609.t1	Q38879	46.8	111	59	0	15	125	22	132	2.1e-29	129.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057609.t1	D8L5E0	95.2	126	6	0	1	126	1	126	9.5e-63	248.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051521	MS.gene057609	0.806309	8.76E-50	-1.69E-46
MS.gene057609	MS.gene057610	0.93028	2.06E-93	-1.69E-46
MS.gene057609	MS.gene058075	0.813103	3.03E-51	-1.69E-46
MS.gene057609	MS.gene059807	0.803804	2.93E-49	-1.69E-46
MS.gene057609	MS.gene06155	0.807368	5.23E-50	-1.69E-46
MS.gene057609	MS.gene065164	0.824344	8.49E-54	-1.69E-46
MS.gene057609	MS.gene065166	0.816002	6.92E-52	-1.69E-46
MS.gene057609	MS.gene066009	0.811112	8.24E-51	-1.69E-46
MS.gene057609	MS.gene066921	0.841767	3.89E-58	-1.69E-46
MS.gene057609	MS.gene068946	0.810716	1.00E-50	-1.69E-46
MS.gene057609	MS.gene069384	0.805025	1.63E-49	-1.69E-46
MS.gene057609	MS.gene069717	0.815239	1.02E-51	-1.69E-46
MS.gene057609	MS.gene071722	0.803265	3.79E-49	-1.69E-46
MS.gene057609	MS.gene08499	0.80475	1.86E-49	-1.69E-46
MS.gene057609	MS.gene20142	0.806928	6.48E-50	-1.69E-46
MS.gene057609	MS.gene21471	0.812984	3.22E-51	-1.69E-46
MS.gene057609	MS.gene25670	0.861506	9.78E-64	-1.69E-46
MS.gene057609	MS.gene25682	0.840291	9.50E-58	-1.69E-46
MS.gene057609	MS.gene26704	0.861997	6.92E-64	-1.69E-46
MS.gene057609	MS.gene34496	0.810812	9.57E-51	-1.69E-46
MS.gene057609	MS.gene36105	0.823719	1.19E-53	-1.69E-46
MS.gene057609	MS.gene36309	0.807556	4.77E-50	-1.69E-46
MS.gene057609	MS.gene40499	0.806521	7.90E-50	-1.69E-46
MS.gene057609	MS.gene41435	0.804528	2.07E-49	-1.69E-46
MS.gene057609	MS.gene42746	0.813861	2.07E-51	-1.69E-46
MS.gene057609	MS.gene42747	0.810845	9.42E-51	-1.69E-46
MS.gene057609	MS.gene43675	0.803369	3.60E-49	-1.69E-46
MS.gene057609	MS.gene47821	0.805871	1.08E-49	-1.69E-46
MS.gene057609	MS.gene49092	0.801106	1.05E-48	-1.69E-46
MS.gene057609	MS.gene49610	0.911416	6.23E-83	-1.69E-46
MS.gene057609	MS.gene49612	0.923627	2.07E-89	-1.69E-46
MS.gene057609	MS.gene52873	0.839221	1.80E-57	-1.69E-46
MS.gene057609	MS.gene52997	0.813446	2.55E-51	-1.69E-46
MS.gene057609	MS.gene58345	0.814903	1.22E-51	-1.69E-46
MS.gene057609	MS.gene58518	0.932261	1.11E-94	-1.69E-46
MS.gene057609	MS.gene58519	0.910052	2.88E-82	-1.69E-46
MS.gene057609	MS.gene67422	0.801447	8.97E-49	-1.69E-46
MS.gene057609	MS.gene70701	0.804041	2.61E-49	-1.69E-46
MS.gene057609	MS.gene74803	0.802351	5.85E-49	-1.69E-46
MS.gene057609	MS.gene74804	0.811321	7.42E-51	-1.69E-46
MS.gene057609	MS.gene74809	0.802715	4.92E-49	-1.69E-46
MS.gene057609	MS.gene89675	0.806781	6.96E-50	-1.69E-46
MS.gene057609	MS.gene98798	0.800762	1.24E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057609.t1	MTR_5g037890	95.238	126	6	0	1	126	1	126	3.27e-80	231
MS.gene057609.t1	MTR_5g037950	65.873	126	42	1	1	125	1	126	1.94e-51	159
MS.gene057609.t1	MTR_5g037930	64.062	128	44	1	1	126	1	128	2.97e-50	156
MS.gene057609.t1	MTR_7g009070	54.717	106	48	0	20	125	28	133	2.95e-42	136
MS.gene057609.t1	MTR_3g112410	47.748	111	58	0	14	124	2	112	1.15e-36	121
MS.gene057609.t1	MTR_1g023140	41.593	113	66	0	14	126	2	114	8.81e-34	114
MS.gene057609.t1	MTR_5g021180	43.243	111	63	0	14	124	4	114	1.36e-33	114
MS.gene057609.t1	MTR_8g107460	41.346	104	61	0	18	121	254	357	2.05e-24	95.9
MS.gene057609.t1	MTR_8g005320	32.381	105	71	0	17	121	25	129	2.96e-22	85.5
MS.gene057609.t1	MTR_7g073680	34.286	105	69	0	17	121	23	127	3.93e-22	85.1
MS.gene057609.t1	MTR_2g010750	37.143	105	66	0	18	122	9	113	7.87e-22	84.0
MS.gene057609.t1	MTR_8g006685	32.381	105	71	0	17	121	94	198	5.43e-21	84.0
MS.gene057609.t1	MTR_8g006560	32.381	105	71	0	17	121	94	198	5.43e-21	84.0
MS.gene057609.t1	MTR_4g081380	35.514	107	65	2	18	122	10	114	6.39e-20	79.3
MS.gene057609.t1	MTR_2g082590	30.476	105	73	0	18	122	10	114	1.56e-18	75.5
MS.gene057609.t1	MTR_2g082590	31.858	113	68	2	10	122	24	127	2.92e-18	75.1
MS.gene057609.t1	MTR_1g098660	33.333	90	58	2	39	126	77	166	8.44e-15	67.0
MS.gene057609.t1	MTR_8g059015	34.091	88	56	2	38	123	87	174	3.77e-14	65.5
MS.gene057609.t1	MTR_7g080250	36.364	88	55	1	40	126	94	181	5.29e-14	65.5
MS.gene057609.t1	MTR_3g089065	27.586	116	79	3	10	123	59	171	6.77e-14	65.1
MS.gene057609.t1	MTR_4g094690	32.927	82	53	1	44	123	85	166	1.19e-13	64.3
MS.gene057609.t1	MTR_2g079420	34.091	88	56	2	38	123	84	171	3.35e-13	63.2
MS.gene057609.t1	MTR_1g114290	31.646	79	50	2	38	115	84	159	2.00e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057609.t1	AT5G39950	46.847	111	59	0	15	125	22	132	6.33e-41	132
MS.gene057609.t1	AT1G69880	47.664	107	56	0	17	123	36	142	3.55e-35	119
MS.gene057609.t1	AT1G59730	39.844	128	74	1	1	125	1	128	8.28e-34	114
MS.gene057609.t1	AT3G51030	41.818	110	64	0	14	123	2	111	8.47e-33	111
MS.gene057609.t1	AT1G19730	44.737	114	62	1	14	126	2	115	6.99e-32	109
MS.gene057609.t1	AT1G45145	42.991	107	61	0	18	124	5	111	6.94e-31	107
MS.gene057609.t1	AT5G42980	42.727	110	63	0	17	126	4	113	1.77e-30	105
MS.gene057609.t1	AT3G17880	39.806	103	62	0	19	121	264	366	5.14e-22	89.7
MS.gene057609.t1	AT3G17880	39.806	103	62	0	19	121	271	373	6.02e-22	89.4
MS.gene057609.t1	AT3G56420	35.870	92	59	0	28	119	50	141	2.67e-20	80.9
MS.gene057609.t1	AT3G56420	35.870	92	59	0	28	119	50	141	2.67e-20	80.9
MS.gene057609.t1	AT3G56420	35.870	92	59	0	28	119	50	141	2.67e-20	80.9
MS.gene057609.t1	AT3G08710	33.333	93	62	0	29	121	34	126	4.35e-19	77.4
MS.gene057609.t1	AT3G08710	33.333	93	62	0	29	121	34	126	4.35e-19	77.4
MS.gene057609.t1	AT3G08710	33.333	93	62	0	29	121	34	126	4.35e-19	77.4
MS.gene057609.t1	AT1G11530	32.381	105	71	0	18	122	2	106	8.63e-18	73.6
MS.gene057609.t1	AT2G40790	33.696	92	61	0	28	119	50	141	3.66e-16	70.5
MS.gene057609.t1	AT2G40790	33.696	92	61	0	28	119	50	141	3.66e-16	70.5
MS.gene057609.t1	AT1G43560	28.906	128	85	3	1	122	33	160	6.90e-16	70.1
MS.gene057609.t1	AT5G16400	37.500	88	54	1	40	126	97	184	2.46e-15	68.9
MS.gene057609.t1	AT3G02730	39.080	87	52	1	41	126	88	174	4.53e-15	68.2
MS.gene057609.t1	AT4G03520	38.554	83	49	2	43	123	101	183	7.35e-15	67.8
MS.gene057609.t1	AT1G03680	38.272	81	48	2	45	123	97	177	1.83e-14	66.6
MS.gene057609.t1	AT1G31020	30.909	110	74	1	16	123	47	156	4.07e-14	65.1
MS.gene057609.t1	AT1G76760	29.474	95	60	3	33	120	70	164	4.89e-14	65.1
MS.gene057609.t1	AT2G35010	36.250	80	47	2	47	123	113	191	8.18e-13	62.4
MS.gene057609.t1	AT2G35010	36.250	80	47	2	47	123	113	191	8.18e-13	62.4
MS.gene057609.t1	AT1G52990	32.967	91	59	2	34	123	222	311	8.16e-12	60.8
MS.gene057609.t1	AT1G50320	35.443	79	47	2	38	115	85	160	1.25e-11	58.9

Find 22 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATTCCATTCCACTGCTAAA+TGG	0.236688	5.4:-27194857	MS.gene057609:CDS
GCTTGATGTTGATGAGTTAT+TGG	0.264813	5.4:-27194605	MS.gene057609:intron
GCAGCAAAATCTTTCAAAAC+TGG	0.287096	5.4:+27194655	None:intergenic
TTATTGATTTCACAGCTAAA+TGG	0.322109	5.4:-27194702	MS.gene057609:CDS
TTTCAAAGAAACTAATAAAC+TGG	0.393712	5.4:-27194817	MS.gene057609:intron
TTCTTAATGAGTAGAAAAGT+TGG	0.438267	5.4:+27194354	None:intergenic
AGGAAAAGTTGTTGATAAAG+TGG	0.478902	5.4:-27194331	MS.gene057609:CDS
TAAAGTGGTGGGAGTAAGAA+AGG	0.479129	5.4:-27194316	MS.gene057609:CDS
GGGAGTTTCTCAAACATTCC+AGG	0.482800	5.4:-27194388	MS.gene057609:CDS
GTGTGGACCTTGCAAAGTTA+TGG	0.487071	5.4:-27194680	MS.gene057609:CDS
ATACACAAAATTATACAAGT+TGG	0.527315	5.4:+27194914	None:intergenic
CATTCCATTTAGCAGTGGAA+TGG	0.530623	5.4:+27194853	None:intergenic
TGGAATGGAATGCAAGGACA+TGG	0.540723	5.4:+27194868	None:intergenic
AAAAGTTGTTGATAAAGTGG+TGG	0.577415	5.4:-27194328	MS.gene057609:CDS
AAGTTGGCAATGAATGCACC+TGG	0.579245	5.4:+27194370	None:intergenic
GATTTCACAGCTAAATGGTG+TGG	0.590907	5.4:-27194697	MS.gene057609:CDS
AAAGTTGTTGATAAAGTGGT+GGG	0.597105	5.4:-27194327	MS.gene057609:CDS
ACTGGATCCATAACTTTGCA+AGG	0.608925	5.4:+27194673	None:intergenic
ATGAGCATTCCATTTAGCAG+TGG	0.609708	5.4:+27194848	None:intergenic
TAGCAGTGGAATGGAATGCA+AGG	0.615008	5.4:+27194862	None:intergenic
TAAGAAAGGAGCAACTACAA+AGG	0.660815	5.4:-27194302	MS.gene057609:CDS
GGAATGGAATGCAAGGACAT+GGG	0.705076	5.4:+27194869	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATAATAGCATTTATAAT+AGG	+	chr5.4:27194729-27194748	None:intergenic	10.0%
!!	ATACACAAAATTATACAAGT+TGG	+	chr5.4:27194288-27194307	None:intergenic	20.0%
!!	TTTCAAAGAAACTAATAAAC+TGG	-	chr5.4:27194382-27194401	MS.gene057609:CDS	20.0%
!!!	TCTTATGTAGTATTTTTCTT+TGG	-	chr5.4:27194465-27194484	MS.gene057609:intron	20.0%
!!!	TGTTTTTTCCACAATATAAA+TGG	-	chr5.4:27194783-27194802	MS.gene057609:intron	20.0%
!	TAACAAACTAGTTAAAGTGA+AGG	+	chr5.4:27194635-27194654	None:intergenic	25.0%
!	TTCTTAATGAGTAGAAAAGT+TGG	+	chr5.4:27194848-27194867	None:intergenic	25.0%
!!	ACTTTTCTACTCATTAAGAA+AGG	-	chr5.4:27194848-27194867	MS.gene057609:CDS	25.0%
!!	GTATTTTTCTTTGGTATAGA+TGG	-	chr5.4:27194474-27194493	MS.gene057609:intron	25.0%
!!!	TTATTGATTTCACAGCTAAA+TGG	-	chr5.4:27194497-27194516	MS.gene057609:intron	25.0%
	AATTAAACCTGGCATAAAGT+TGG	+	chr5.4:27194669-27194688	None:intergenic	30.0%
!	AAAAGTTGTTGATAAAGTGG+TGG	-	chr5.4:27194871-27194890	MS.gene057609:CDS	30.0%
!	AAAGTTGTTGATAAAGTGGT+GGG	-	chr5.4:27194872-27194891	MS.gene057609:CDS	30.0%
!	AGGAAAAGTTGTTGATAAAG+TGG	-	chr5.4:27194868-27194887	MS.gene057609:CDS	30.0%
	ACCAACTGAGTAATTAAACC+TGG	+	chr5.4:27194680-27194699	None:intergenic	35.0%
	CCACAATATAAATGGCAACA+GGG	-	chr5.4:27194791-27194810	MS.gene057609:intron	35.0%
	GCAGCAAAATCTTTCAAAAC+TGG	+	chr5.4:27194547-27194566	None:intergenic	35.0%
	TAAGAAAGGAGCAACTACAA+AGG	-	chr5.4:27194897-27194916	MS.gene057609:CDS	35.0%
	TCCACAATATAAATGGCAAC+AGG	-	chr5.4:27194790-27194809	MS.gene057609:intron	35.0%
	TGTAAAACCAACTTTATGCC+AGG	-	chr5.4:27194659-27194678	MS.gene057609:CDS	35.0%
!	GCTTGATGTTGATGAGTTAT+TGG	-	chr5.4:27194594-27194613	MS.gene057609:intron	35.0%
	ACTGGATCCATAACTTTGCA+AGG	+	chr5.4:27194529-27194548	None:intergenic	40.0%
	ATGAGCATTCCATTTAGCAG+TGG	+	chr5.4:27194354-27194373	None:intergenic	40.0%
	CATTCCATTCCACTGCTAAA+TGG	-	chr5.4:27194342-27194361	MS.gene057609:CDS	40.0%
	CATTCCATTTAGCAGTGGAA+TGG	+	chr5.4:27194349-27194368	None:intergenic	40.0%
	GATTTCACAGCTAAATGGTG+TGG	-	chr5.4:27194502-27194521	MS.gene057609:intron	40.0%
	GCCAGGTTTAATTACTCAGT+TGG	-	chr5.4:27194676-27194695	MS.gene057609:CDS	40.0%
!	CCCTGTTGCCATTTATATTG+TGG	+	chr5.4:27194794-27194813	None:intergenic	40.0%
!	TAAAGTGGTGGGAGTAAGAA+AGG	-	chr5.4:27194883-27194902	MS.gene057609:CDS	40.0%
	AAGTTGGCAATGAATGCACC+TGG	+	chr5.4:27194832-27194851	None:intergenic	45.0%
	GGAATGGAATGCAAGGACAT+GGG	+	chr5.4:27194333-27194352	None:intergenic	45.0%
	GGGAGTTTCTCAAACATTCC+AGG	-	chr5.4:27194811-27194830	MS.gene057609:intron	45.0%
	GTGTGGACCTTGCAAAGTTA+TGG	-	chr5.4:27194519-27194538	MS.gene057609:intron	45.0%
	TAGCAGTGGAATGGAATGCA+AGG	+	chr5.4:27194340-27194359	None:intergenic	45.0%
	TGGAATGGAATGCAAGGACA+TGG	+	chr5.4:27194334-27194353	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	27194278	27194943	27194278	ID=MS.gene057609
chr5.4	mRNA	27194278	27194943	27194278	ID=MS.gene057609.t1;Parent=MS.gene057609
chr5.4	exon	27194818	27194943	27194818	ID=MS.gene057609.t1.exon1;Parent=MS.gene057609.t1
chr5.4	CDS	27194818	27194943	27194818	ID=cds.MS.gene057609.t1;Parent=MS.gene057609.t1
chr5.4	exon	27194606	27194728	27194606	ID=MS.gene057609.t1.exon2;Parent=MS.gene057609.t1
chr5.4	CDS	27194606	27194728	27194606	ID=cds.MS.gene057609.t1;Parent=MS.gene057609.t1
chr5.4	exon	27194278	27194409	27194278	ID=MS.gene057609.t1.exon3;Parent=MS.gene057609.t1
chr5.4	CDS	27194278	27194409	27194278	ID=cds.MS.gene057609.t1;Parent=MS.gene057609.t1

Gene Sequence

>MS.gene057609

ATGAGTTCCAACTTGTATAATTTTGTGTATGTTGATAGATCAACAGAATCATCCCATGTCCTTGCATTCCATTCCACTGCTAAATGGAATGCTCATTTTGAAGCTTTCAAAGAAACTAATAAACTGGTATGTTTGCGTAGCTAAAATCTTAACTAGCTATACAATCTTAGTATATAAAATTTAGTTTTCTTATGTAGTATTTTTCTTTGGTATAGATGGTTATTGATTTCACAGCTAAATGGTGTGGACCTTGCAAAGTTATGGATCCAGTTTTGAAAGATTTTGCTGCAAAATACACTGATGTTGAGTTCATTAAGCTTGATGTTGATGAGTTATTGGTATGTTACAGATTATCCTTCACTTTAACTAGTTTGTTATTTATGTAAAACCAACTTTATGCCAGGTTTAATTACTCAGTTGGTTTCTAAACTATTTGAATTTTTTTGAACCTATTATAAATGCTATTATTTTTTAGAAACATGTTTTCAACACTTGAGAACTGATTTGTTTTTTCCACAATATAAATGGCAACAGGGAGTTTCTCAAACATTCCAGGTGCATTCATTGCCAACTTTTCTACTCATTAAGAAAGGAAAAGTTGTTGATAAAGTGGTGGGAGTAAGAAAGGAGCAACTACAAAGGATGATTGAAAAGCAAAGAAACTAA

Protein sequence

>MS.gene057609.t1

MSSNLYNFVYVDRSTESSHVLAFHSTAKWNAHFEAFKETNKLMVIDFTAKWCGPCKVMDPVLKDFAAKYTDVEFIKLDVDELLGVSQTFQVHSLPTFLLIKKGKVVDKVVGVRKEQLQRMIEKQRN