AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59238

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59238.t1 MTR_5g081040 97.872 235 5 0 1 235 39 273 1.58e-169 468
MS.gene59238.t1 MTR_5g081040 98.095 210 4 0 26 235 4 213 1.25e-148 412
MS.gene59238.t1 MTR_3g069150 78.298 235 49 2 1 234 39 272 2.89e-128 363
MS.gene59238.t1 MTR_3g069170 78.218 202 43 1 1 201 39 240 2.52e-111 320
MS.gene59238.t1 MTR_3g069150 78.035 173 37 1 1 172 39 211 2.10e-94 277
MS.gene59238.t1 MTR_7g113620 38.140 215 124 3 1 208 38 250 1.61e-48 161
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59238.t1 AT2G39805 65.106 235 78 3 1 234 43 274 4.78e-109 315
MS.gene59238.t1 AT2G39805 64.831 236 78 4 1 234 43 275 2.65e-107 310
MS.gene59238.t1 AT5G27490 38.272 243 139 5 1 234 38 278 1.05e-51 169
MS.gene59238.t1 AT3G05280 37.118 229 132 4 1 220 37 262 2.41e-46 155

Find 62 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGTAAGACCTCAAGTTTA+AGG 0.239012 5.2:+68742840 None:intergenic
ATGCTAACCTTATAAGGTTT+TGG 0.250616 5.2:-68743214 MS.gene59238:CDS
GTTCAAATGCTAACCTTATA+AGG 0.256753 5.2:-68743220 MS.gene59238:CDS
ACATGCACCAAAACCTTATA+AGG 0.322668 5.2:+68743207 None:intergenic
ATTGTAGATACGGGCTTATA+TGG 0.331360 5.2:-68743430 MS.gene59238:intron
TACTGAAGAGATGATGAAAA+TGG 0.347950 5.2:+68743169 None:intergenic
GTCTTTGTACTTGCCTCGCT+TGG 0.388742 5.2:-68743392 MS.gene59238:CDS
ACGGCTGCATGGTCTATCTA+TGG 0.398905 5.2:-68743293 MS.gene59238:CDS
CTACTTCTTCCTTCAGTACA+TGG 0.404025 5.2:-68743243 MS.gene59238:CDS
TGGCAGTCTTCATCAAGGTT+TGG 0.405808 5.2:-68742762 MS.gene59238:CDS
CTGAAGAAGTCTCCACCAAC+TGG 0.422517 5.2:+68743776 None:intergenic
CTGTCTCCTCCGTTGGTGGC+GGG 0.428585 5.2:+68745422 None:intergenic
TGATGTTAGCTATATGAATA+CGG 0.435253 5.2:-68743312 MS.gene59238:CDS
AACAACAGCCAACAAACAAC+TGG 0.436583 5.2:-68743886 MS.gene59238:CDS
TGGATCATAACACTCATTAC+TGG 0.437777 5.2:-68742886 MS.gene59238:CDS
ATTGAAGGAACTTATCAATC+TGG 0.438429 5.2:+68743799 None:intergenic
TCTGTCTCCTCCGTTGGTGG+CGG 0.440294 5.2:+68745421 None:intergenic
TCCTCTGTCTCCTCCGTTGG+TGG 0.443214 5.2:+68745418 None:intergenic
TATCTATGGTTATGCAATAG+TGG 0.444016 5.2:-68743279 MS.gene59238:CDS
GTCTCCACCAACTGGATTGA+AGG 0.450273 5.2:+68743784 None:intergenic
CAAACTGTTGGCACTCCTAC+TGG 0.454589 5.2:-68745383 MS.gene59238:intron
TGACATTTCAGTGGCGATCA+TGG 0.461393 5.2:-68742809 MS.gene59238:CDS
TATCTGCATGTTGTTGCATA+AGG 0.465432 5.2:+68743354 None:intergenic
ATCCACCGCAGAATCTCAAC+TGG 0.468604 5.2:+68742904 None:intergenic
CAACGGAGGAGACAGAGGAC+GGG 0.471171 5.2:-68745414 MS.gene59238:CDS
CCGTCCTCTGTCTCCTCCGT+TGG 0.479438 5.2:+68745415 None:intergenic
CTCCTCCGTTGGTGGCGGGA+GGG 0.480975 5.2:+68745426 None:intergenic
TCTCCTCCGTTGGTGGCGGG+AGG 0.493774 5.2:+68745425 None:intergenic
AACGGAGGAGACAGAGGACG+GGG 0.501183 5.2:-68745413 MS.gene59238:CDS
GCTTTGTTGCCTTAAACTTG+AGG 0.501740 5.2:-68742849 MS.gene59238:CDS
CAAGTTTAAGGCAACAAAGC+TGG 0.520009 5.2:+68742852 None:intergenic
AGGAAGAAGTAGTATGCCAT+TGG 0.521354 5.2:+68743254 None:intergenic
AGTTCCTTCAATCCAGTTGG+TGG 0.527765 5.2:-68743788 MS.gene59238:CDS
CCAACGGAGGAGACAGAGGA+CGG 0.529195 5.2:-68745415 MS.gene59238:CDS
TACTTCTTCCTTCAGTACAT+GGG 0.549442 5.2:-68743242 MS.gene59238:CDS
GGAAGAAGTAGTATGCCATT+GGG 0.550097 5.2:+68743255 None:intergenic
ATAAGTTCCTTCAATCCAGT+TGG 0.554032 5.2:-68743791 MS.gene59238:CDS
ATGCAGATAGCGGTACCTCA+TGG 0.555755 5.2:-68743340 MS.gene59238:CDS
AGGTCTGGCAGTCTTCATCA+AGG 0.563444 5.2:-68742767 MS.gene59238:CDS
CATTTGAACCCATGTACTGA+AGG 0.576053 5.2:+68743234 None:intergenic
CGGGGAGGATATCAAACTGT+TGG 0.580950 5.2:-68745395 MS.gene59238:CDS
GCTATATGAATACGGCTGCA+TGG 0.592343 5.2:-68743304 MS.gene59238:CDS
AACAGCCAACAAACAACTGG+AGG 0.595623 5.2:-68743883 MS.gene59238:CDS
ATATTCCCTCCCGCCACCAA+CGG 0.595751 5.2:-68745431 MS.gene59238:CDS
TATATGGATCTCAACGACAT+TGG 0.600089 5.2:-68743414 MS.gene59238:CDS
TTCCCTCCCGCCACCAACGG+AGG 0.601298 5.2:-68745428 MS.gene59238:CDS
GCCACCAACGGAGGAGACAG+AGG 0.602397 5.2:-68745419 MS.gene59238:CDS
GGAGGAGACAGAGGACGGGG+AGG 0.603333 5.2:-68745410 MS.gene59238:CDS
AGCCAACAAACAACTGGAGG+GGG 0.616047 5.2:-68743880 MS.gene59238:CDS
ACTCATTACTGGTGTTGCGT+CGG 0.617025 5.2:-68742875 MS.gene59238:CDS
AAGAGATGATGAAAATGGAG+AGG 0.620309 5.2:+68743174 None:intergenic
TAATTCCAGTTGAGATTCTG+CGG 0.620402 5.2:-68742909 MS.gene59238:CDS
AGAGATGATGAAAATGGAGA+GGG 0.621172 5.2:+68743175 None:intergenic
TTATGCAATAGTGGTCCCAA+TGG 0.635472 5.2:-68743270 MS.gene59238:CDS
ACAGCCAACAAACAACTGGA+GGG 0.635567 5.2:-68743882 MS.gene59238:CDS
CAGCCAACAAACAACTGGAG+GGG 0.648127 5.2:-68743881 MS.gene59238:CDS
AGAAAGCAATGACATTTCAG+TGG 0.681324 5.2:-68742818 MS.gene59238:CDS
TTCCAGTTGAGATTCTGCGG+TGG 0.686062 5.2:-68742906 MS.gene59238:CDS
ATGCAACAACATGCAGATAG+CGG 0.691113 5.2:-68743350 MS.gene59238:CDS
TGTGGCAAGATTTCCAAGCG+AGG 0.696815 5.2:+68743379 None:intergenic
GCTAACATCAAAGCTCCATG+AGG 0.728832 5.2:+68743325 None:intergenic
ATGTTGTTGCATAAGGAATG+TGG 0.728927 5.2:+68743361 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAACAATTAGATTAAC+TGG + chr5.2:68744119-68744138 None:intergenic 15.0%
!! AAGTAAATAAACTATACAAA+TGG + chr5.2:68743341-68743360 None:intergenic 15.0%
!! ATGAAGTATATACAATTTAA+AGG + chr5.2:68742904-68742923 None:intergenic 15.0%
!! CAATTAATAATAAGTCATAT+TGG + chr5.2:68743773-68743792 None:intergenic 15.0%
!! CTATCTAATTTATATTACTT+GGG - chr5.2:68745235-68745254 MS.gene59238:intron 15.0%
!!! TTTTTATGTTATTGTAGATA+CGG - chr5.2:68744747-68744766 MS.gene59238:intron 15.0%
!! ACTATTTATTACTAACTGTA+TGG - chr5.2:68744720-68744739 MS.gene59238:intron 20.0%
!! ACTCGAAATCTTTAATTAAA+TGG + chr5.2:68742966-68742985 None:intergenic 20.0%
!! ATGTATCCATAAAAAATAAG+TGG + chr5.2:68744615-68744634 None:intergenic 20.0%
!! ATTTAATTAAAGATTTCGAG+TGG - chr5.2:68742965-68742984 MS.gene59238:intron 20.0%
!! CAAATGACTAAAAATACAAA+TGG + chr5.2:68744223-68744242 None:intergenic 20.0%
!! GCTATCTAATTTATATTACT+TGG - chr5.2:68745234-68745253 MS.gene59238:intron 20.0%
!! TATATAACAGTTTATATTGC+AGG + chr5.2:68743967-68743986 None:intergenic 20.0%
!!! ATATATTTGTTGTATTTCCA+TGG - chr5.2:68742860-68742879 MS.gene59238:CDS 20.0%
!!! TTTTATGTTATTGTAGATAC+GGG - chr5.2:68744748-68744767 MS.gene59238:intron 20.0%
! AAAAAAAAAACGCACAAATC+AGG + chr5.2:68744453-68744472 None:intergenic 25.0%
! AAAAAAAAACGCACAAATCA+GGG + chr5.2:68744452-68744471 None:intergenic 25.0%
! AATAGAGTTGCAAATCTTAA+TGG - chr5.2:68744521-68744540 MS.gene59238:intron 25.0%
! AGTAGAGATTGAAAAGTAAA+TGG + chr5.2:68743063-68743082 None:intergenic 25.0%
! AGTATTATATTGTGCAATAG+TGG - chr5.2:68743474-68743493 MS.gene59238:intron 25.0%
! ATACATTTACTGAACTTATC+CGG - chr5.2:68744650-68744669 MS.gene59238:intron 25.0%
! ATAGAGTTGCAAATCTTAAT+GGG - chr5.2:68744522-68744541 MS.gene59238:intron 25.0%
! GTAGAGATTGAAAAGTAAAT+GGG + chr5.2:68743062-68743081 None:intergenic 25.0%
! TAATGTCATTCACTAATTTC+TGG - chr5.2:68744252-68744271 MS.gene59238:intron 25.0%
! TAATTCCTATTATTGAGAAG+AGG + chr5.2:68743687-68743706 None:intergenic 25.0%
! TAGCTAGTAAAACATTTACA+AGG + chr5.2:68743204-68743223 None:intergenic 25.0%
! TATAGTACATGAAAAATCGT+AGG + chr5.2:68743024-68743043 None:intergenic 25.0%
! TCTTCAATGAAAAGGAAAAA+GGG + chr5.2:68743597-68743616 None:intergenic 25.0%
! TGATGTTAGCTATATGAATA+CGG - chr5.2:68744875-68744894 MS.gene59238:intron 25.0%
! TTCAATGTAATTCACAAAGT+AGG + chr5.2:68744040-68744059 None:intergenic 25.0%
! TTTACTGTTATTATGTTCAC+TGG - chr5.2:68743997-68744016 MS.gene59238:intron 25.0%
!! AACTTACTTTAGCATTTTGT+AGG - chr5.2:68744566-68744585 MS.gene59238:intron 25.0%
!! AGATTTTCTCTTCAATGAAA+AGG + chr5.2:68743605-68743624 None:intergenic 25.0%
!! ATGCACAATCAATGAATTTT+GGG + chr5.2:68744693-68744712 None:intergenic 25.0%
!!! TATACTCTCATTTTTTACCA+AGG - chr5.2:68744158-68744177 MS.gene59238:intron 25.0%
AACTCCGCAATTTATAACAA+AGG - chr5.2:68743554-68743573 MS.gene59238:intron 30.0%
AAGAAATTGCATCATTTGCT+AGG - chr5.2:68745188-68745207 MS.gene59238:intron 30.0%
ACAATTTAAAGGCTTTCATG+AGG + chr5.2:68742893-68742912 None:intergenic 30.0%
ACCTATTTGCAAGAAAAATG+CGG + chr5.2:68745404-68745423 None:intergenic 30.0%
ATTGAAGGAACTTATCAATC+TGG + chr5.2:68744391-68744410 None:intergenic 30.0%
CAATTTATAACAAAGGAGCT+GGG - chr5.2:68743561-68743580 MS.gene59238:intron 30.0%
CCATAAAAAATAAGTGGACA+AGG + chr5.2:68744609-68744628 None:intergenic 30.0%
CTCTTCAATGAAAAGGAAAA+AGG + chr5.2:68743598-68743617 None:intergenic 30.0%
GGATACATGTATTAAGTCAT+GGG - chr5.2:68744627-68744646 MS.gene59238:intron 30.0%
GTATATGTAAACCTTGAAGA+TGG + chr5.2:68743945-68743964 None:intergenic 30.0%
GTTCAAATGCTAACCTTATA+AGG - chr5.2:68744967-68744986 MS.gene59238:intron 30.0%
TACATTTACTGAACTTATCC+GGG - chr5.2:68744651-68744670 MS.gene59238:intron 30.0%
TACTGAAGAGATGATGAAAA+TGG + chr5.2:68745021-68745040 None:intergenic 30.0%
TAGAGTTGCAAATCTTAATG+GGG - chr5.2:68744523-68744542 MS.gene59238:intron 30.0%
TATCTATGGTTATGCAATAG+TGG - chr5.2:68744908-68744927 MS.gene59238:intron 30.0%
TATGTAAGACCTCAAGTTTA+AGG + chr5.2:68745350-68745369 None:intergenic 30.0%
TCAGTACAATATTAGGTTTG+AGG + chr5.2:68743368-68743387 None:intergenic 30.0%
TGGATACATGTATTAAGTCA+TGG - chr5.2:68744626-68744645 MS.gene59238:intron 30.0%
TTGTTATAAATTGCGGAGTT+TGG + chr5.2:68743554-68743573 None:intergenic 30.0%
! AGCTCCTTTGTTATAAATTG+CGG + chr5.2:68743561-68743580 None:intergenic 30.0%
! ATGCTAACCTTATAAGGTTT+TGG - chr5.2:68744973-68744992 MS.gene59238:intron 30.0%
! ATTACTCTACTATTGGTTGA+GGG - chr5.2:68743131-68743150 MS.gene59238:intron 30.0%
! ATTTTTCTTGCAAATAGGTC+TGG - chr5.2:68745405-68745424 MS.gene59238:CDS 30.0%
! CATGCACAATCAATGAATTT+TGG + chr5.2:68744694-68744713 None:intergenic 30.0%
! CTTTGTAATGAATTGATGAC+CGG - chr5.2:68742926-68742945 MS.gene59238:intron 30.0%
! TATTGAAAGTATAGAAGCAC+AGG + chr5.2:68744496-68744515 None:intergenic 30.0%
!!! ATGTTAGTTTGTTTTACCGA+GGG + chr5.2:68743830-68743849 None:intergenic 30.0%
!!! CCTTGTCCACTTATTTTTTA+TGG - chr5.2:68744606-68744625 MS.gene59238:intron 30.0%
AAACCAATTATGATGCGATC+TGG - chr5.2:68743105-68743124 MS.gene59238:intron 35.0%
AACCAATTATGATGCGATCT+GGG - chr5.2:68743106-68743125 MS.gene59238:intron 35.0%
AAGAGATGATGAAAATGGAG+AGG + chr5.2:68745016-68745035 None:intergenic 35.0%
AATTGCGGAGTTTGGAAAAT+AGG + chr5.2:68743546-68743565 None:intergenic 35.0%
ACAAAATTCATCCCAGTTGT+TGG + chr5.2:68745065-68745084 None:intergenic 35.0%
ACATGCACCAAAACCTTATA+AGG + chr5.2:68744983-68745002 None:intergenic 35.0%
ACATTTACTGAACTTATCCG+GGG - chr5.2:68744652-68744671 MS.gene59238:intron 35.0%
AGAAAGCAATGACATTTCAG+TGG - chr5.2:68745369-68745388 MS.gene59238:intron 35.0%
AGAGATGATGAAAATGGAGA+GGG + chr5.2:68745015-68745034 None:intergenic 35.0%
AGAGTTGCAAATCTTAATGG+GGG - chr5.2:68744524-68744543 MS.gene59238:intron 35.0%
ATAAGTTCCTTCAATCCAGT+TGG - chr5.2:68744396-68744415 MS.gene59238:intron 35.0%
ATGTTGTTGCATAAGGAATG+TGG + chr5.2:68744829-68744848 None:intergenic 35.0%
ATTGCGGAGTTTGGAAAATA+GGG + chr5.2:68743545-68743564 None:intergenic 35.0%
ATTGTAGATACGGGCTTATA+TGG - chr5.2:68744757-68744776 MS.gene59238:intron 35.0%
CAAAATTCATCCCAGTTGTT+GGG + chr5.2:68745064-68745083 None:intergenic 35.0%
CAGAATCAAATCAACAACCT+TGG + chr5.2:68744178-68744197 None:intergenic 35.0%
CTTGAGATATGCATTACTAG+TGG + chr5.2:68744083-68744102 None:intergenic 35.0%
GAAGTAGAACTGCTTGATTA+TGG - chr5.2:68743300-68743319 MS.gene59238:CDS 35.0%
GCAATTTATAACAAAGGAGC+TGG - chr5.2:68743560-68743579 MS.gene59238:intron 35.0%
TAATTCCAGTTGAGATTCTG+CGG - chr5.2:68745278-68745297 MS.gene59238:intron 35.0%
TACTTCTTCCTTCAGTACAT+GGG - chr5.2:68744945-68744964 MS.gene59238:intron 35.0%
TATATGGATCTCAACGACAT+TGG - chr5.2:68744773-68744792 MS.gene59238:intron 35.0%
TATCTGCATGTTGTTGCATA+AGG + chr5.2:68744836-68744855 None:intergenic 35.0%
TCCTAAACCCCAAATAACAA+GGG + chr5.2:68743272-68743291 None:intergenic 35.0%
TGGATCATAACACTCATTAC+TGG - chr5.2:68745301-68745320 MS.gene59238:intron 35.0%
TTTCATGAGGAAAAAAGCCA+TGG + chr5.2:68742880-68742899 None:intergenic 35.0%
! ACAAATGGAACATTCTGAGA+CGG + chr5.2:68743326-68743345 None:intergenic 35.0%
! CATTACTCTACTATTGGTTG+AGG - chr5.2:68743130-68743149 MS.gene59238:intron 35.0%
! TAAAGATTTCGAGTGGATAC+TGG - chr5.2:68742972-68742991 MS.gene59238:intron 35.0%
!! TACAAATGGCAGCTATTTTC+AGG + chr5.2:68744209-68744228 None:intergenic 35.0%
!! TATAAGGTTTTGGTGCATGT+GGG - chr5.2:68744983-68745002 MS.gene59238:intron 35.0%
!! TTATAAGGTTTTGGTGCATG+TGG - chr5.2:68744982-68745001 MS.gene59238:intron 35.0%
!!! CATGTTAGTTTGTTTTACCG+AGG + chr5.2:68743831-68743850 None:intergenic 35.0%
!!! TGTTAGTTTGTTTTACCGAG+GGG + chr5.2:68743829-68743848 None:intergenic 35.0%
AACAACAGCCAACAAACAAC+TGG - chr5.2:68744301-68744320 MS.gene59238:intron 40.0%
AATAACAAGGGGAAAGAGGA+AGG + chr5.2:68743260-68743279 None:intergenic 40.0%
AATGGATCATTTCTCCACAC+CGG + chr5.2:68742948-68742967 None:intergenic 40.0%
ACTATAGCGCTATTGCATAG+TGG - chr5.2:68743412-68743431 MS.gene59238:CDS 40.0%
AGCTGCCTCTTCTCAATAAT+AGG - chr5.2:68743679-68743698 MS.gene59238:intron 40.0%
AGGAAGAAGTAGTATGCCAT+TGG + chr5.2:68744936-68744955 None:intergenic 40.0%
ATGCAACAACATGCAGATAG+CGG - chr5.2:68744837-68744856 MS.gene59238:intron 40.0%
CAAGCAGTTCTACTTCAAAC+TGG + chr5.2:68743296-68743315 None:intergenic 40.0%
CATTTGAACCCATGTACTGA+AGG + chr5.2:68744956-68744975 None:intergenic 40.0%
CCTAAACCCCAAATAACAAG+GGG + chr5.2:68743271-68743290 None:intergenic 40.0%
CTACTTCTTCCTTCAGTACA+TGG - chr5.2:68744944-68744963 MS.gene59238:intron 40.0%
CTCCTAAACCCCAAATAACA+AGG + chr5.2:68743273-68743292 None:intergenic 40.0%
CTCCTACTGGTATGTTATCT+AGG - chr5.2:68742817-68742836 MS.gene59238:CDS 40.0%
CTGGGTCATTACTCTACTAT+TGG - chr5.2:68743124-68743143 MS.gene59238:intron 40.0%
GACCTAGATAACATACCAGT+AGG + chr5.2:68742822-68742841 None:intergenic 40.0%
GAGTTGCAAATCTTAATGGG+GGG - chr5.2:68744525-68744544 MS.gene59238:intron 40.0%
GGAAATGATCCTTAAAACCC+CGG + chr5.2:68744672-68744691 None:intergenic 40.0%
GGAAGAAGTAGTATGCCATT+GGG + chr5.2:68744935-68744954 None:intergenic 40.0%
GTTGTTCAAATGCTTCTGCA+TGG + chr5.2:68744287-68744306 None:intergenic 40.0%
TTATGCAATAGTGGTCCCAA+TGG - chr5.2:68744917-68744936 MS.gene59238:intron 40.0%
TTGCGGAGTTTGGAAAATAG+GGG + chr5.2:68743544-68743563 None:intergenic 40.0%
! CAAGTTTAAGGCAACAAAGC+TGG + chr5.2:68745338-68745357 None:intergenic 40.0%
! CTCTGGCTCAGTACAATATT+AGG + chr5.2:68743375-68743394 None:intergenic 40.0%
! GCCGCATTTTTCTTGCAAAT+AGG - chr5.2:68745400-68745419 MS.gene59238:CDS 40.0%
! GCTTTGTTGCCTTAAACTTG+AGG - chr5.2:68745338-68745357 MS.gene59238:intron 40.0%
! TAATGAATTGATGACCGGTG+TGG - chr5.2:68742931-68742950 MS.gene59238:intron 40.0%
! TGAACTTATCCGGGGTTTTA+AGG - chr5.2:68744660-68744679 MS.gene59238:intron 40.0%
!! ATAAGGTTTTGGTGCATGTG+GGG - chr5.2:68744984-68745003 MS.gene59238:intron 40.0%
!! CCTCTTTCCCCTTGTTATTT+GGG - chr5.2:68743261-68743280 MS.gene59238:CDS 40.0%
!! CTCTTTCCCCTTGTTATTTG+GGG - chr5.2:68743262-68743281 MS.gene59238:CDS 40.0%
!! TCCTCTTTCCCCTTGTTATT+TGG - chr5.2:68743260-68743279 MS.gene59238:CDS 40.0%
AACAGCCAACAAACAACTGG+AGG - chr5.2:68744304-68744323 MS.gene59238:intron 45.0%
ACAGCCAACAAACAACTGGA+GGG - chr5.2:68744305-68744324 MS.gene59238:intron 45.0%
AGTTCCTTCAATCCAGTTGG+TGG - chr5.2:68744399-68744418 MS.gene59238:intron 45.0%
CCCAAATAACAAGGGGAAAG+AGG + chr5.2:68743264-68743283 None:intergenic 45.0%
GACCCAGATCGCATCATAAT+TGG + chr5.2:68743111-68743130 None:intergenic 45.0%
GCAACTGTGTACCATCTTCA+AGG - chr5.2:68743931-68743950 MS.gene59238:intron 45.0%
GCCAGAGTGTAGTTAGATAG+CGG - chr5.2:68743388-68743407 MS.gene59238:CDS 45.0%
GCTAACATCAAAGCTCCATG+AGG + chr5.2:68744865-68744884 None:intergenic 45.0%
GCTATATGAATACGGCTGCA+TGG - chr5.2:68744883-68744902 MS.gene59238:intron 45.0%
TCTTCCTAAGCCCAACAACT+GGG - chr5.2:68745051-68745070 MS.gene59238:intron 45.0%
TGACATTTCAGTGGCGATCA+TGG - chr5.2:68745378-68745397 MS.gene59238:intron 45.0%
TGGCAGTCTTCATCAAGGTT+TGG - chr5.2:68745425-68745444 MS.gene59238:CDS 45.0%
TTCTTCCTAAGCCCAACAAC+TGG - chr5.2:68745050-68745069 MS.gene59238:intron 45.0%
TTGTGCAATAGTGGCTACAG+CGG - chr5.2:68743483-68743502 MS.gene59238:intron 45.0%
!! ACTCATTACTGGTGTTGCGT+CGG - chr5.2:68745312-68745331 MS.gene59238:intron 45.0%
!! CCCCTTGTTATTTGGGGTTT+AGG - chr5.2:68743268-68743287 MS.gene59238:CDS 45.0%
AACCCCCTCCAGTTGTTTGT+TGG + chr5.2:68744312-68744331 None:intergenic 50.0%
ACGGCTGCATGGTCTATCTA+TGG - chr5.2:68744894-68744913 MS.gene59238:intron 50.0%
AGCCAACAAACAACTGGAGG+GGG - chr5.2:68744307-68744326 MS.gene59238:intron 50.0%
AGGTCTGGCAGTCTTCATCA+AGG - chr5.2:68745420-68745439 MS.gene59238:CDS 50.0%
ATCCACCGCAGAATCTCAAC+TGG + chr5.2:68745286-68745305 None:intergenic 50.0%
CAAACTGTTGGCACTCCTAC+TGG - chr5.2:68742804-68742823 MS.gene59238:CDS 50.0%
CAGCCAACAAACAACTGGAG+GGG - chr5.2:68744306-68744325 MS.gene59238:intron 50.0%
CGGGGAGGATATCAAACTGT+TGG - chr5.2:68742792-68742811 MS.gene59238:CDS 50.0%
CTGAAGAAGTCTCCACCAAC+TGG + chr5.2:68744414-68744433 None:intergenic 50.0%
GCCGCTATCTAACTACACTC+TGG + chr5.2:68743392-68743411 None:intergenic 50.0%
GTCTCCACCAACTGGATTGA+AGG + chr5.2:68744406-68744425 None:intergenic 50.0%
GTCTTTGTACTTGCCTCGCT+TGG - chr5.2:68744795-68744814 MS.gene59238:intron 50.0%
TCATCCCAGTTGTTGGGCTT+AGG + chr5.2:68745058-68745077 None:intergenic 50.0%
TGTGGCAAGATTTCCAAGCG+AGG + chr5.2:68744811-68744830 None:intergenic 50.0%
TTCCAGTTGAGATTCTGCGG+TGG - chr5.2:68745281-68745300 MS.gene59238:intron 50.0%
! ATGCAGATAGCGGTACCTCA+TGG - chr5.2:68744847-68744866 MS.gene59238:intron 50.0%
ATATTCCCTCCCGCCACCAA+CGG - chr5.2:68742756-68742775 MS.gene59238:CDS 55.0%
CTCAATCATCAGCTGCCCCT+CGG - chr5.2:68743811-68743830 MS.gene59238:CDS 55.0%
AACGGAGGAGACAGAGGACG+GGG - chr5.2:68742774-68742793 MS.gene59238:CDS 60.0%
CAACGGAGGAGACAGAGGAC+GGG - chr5.2:68742773-68742792 MS.gene59238:CDS 60.0%
CCAACGGAGGAGACAGAGGA+CGG - chr5.2:68742772-68742791 MS.gene59238:CDS 60.0%
TCCTCTGTCTCCTCCGTTGG+TGG + chr5.2:68742772-68742791 None:intergenic 60.0%
! TCTGTCTCCTCCGTTGGTGG+CGG + chr5.2:68742769-68742788 None:intergenic 60.0%
CCGTCCTCTGTCTCCTCCGT+TGG + chr5.2:68742775-68742794 None:intergenic 65.0%
GCCACCAACGGAGGAGACAG+AGG - chr5.2:68742768-68742787 MS.gene59238:CDS 65.0%
!! CTGTCTCCTCCGTTGGTGGC+GGG + chr5.2:68742768-68742787 None:intergenic 65.0%
GGAGGAGACAGAGGACGGGG+AGG - chr5.2:68742777-68742796 MS.gene59238:CDS 70.0%
TTCCCTCCCGCCACCAACGG+AGG - chr5.2:68742759-68742778 MS.gene59238:CDS 70.0%
!! CTCCTCCGTTGGTGGCGGGA+GGG + chr5.2:68742764-68742783 None:intergenic 70.0%
!! TCTCCTCCGTTGGTGGCGGG+AGG + chr5.2:68742765-68742784 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 68742750 68745459 68742750 ID=MS.gene59238
chr5.2 mRNA 68742750 68745459 68742750 ID=MS.gene59238.t1;Parent=MS.gene59238
chr5.2 exon 68745384 68745459 68745384 ID=MS.gene59238.t1.exon1;Parent=MS.gene59238.t1
chr5.2 CDS 68745384 68745459 68745384 ID=cds.MS.gene59238.t1;Parent=MS.gene59238.t1
chr5.2 exon 68743752 68743917 68743752 ID=MS.gene59238.t1.exon2;Parent=MS.gene59238.t1
chr5.2 CDS 68743752 68743917 68743752 ID=cds.MS.gene59238.t1;Parent=MS.gene59238.t1
chr5.2 exon 68743172 68743445 68743172 ID=MS.gene59238.t1.exon3;Parent=MS.gene59238.t1
chr5.2 CDS 68743172 68743445 68743172 ID=cds.MS.gene59238.t1;Parent=MS.gene59238.t1
chr5.2 exon 68742750 68742941 68742750 ID=MS.gene59238.t1.exon4;Parent=MS.gene59238.t1
chr5.2 CDS 68742750 68742941 68742750 ID=cds.MS.gene59238.t1;Parent=MS.gene59238.t1
Gene Sequence

>MS.gene59238

ATGCAAATATTCCCTCCCGCCACCAACGGAGGAGACAGAGGACGGGGAGGATATCAAACTGTTGGCACTCCTACTGGTATGTTATCTAGGTCGCTGTTTTTTATTTGACAATATATTTGTTGTATTTCCATGGCTTTTTTCCTCATGAAAGCCTTTAAATTGTATATACTTCATTACTTTGTAATGAATTGATGACCGGTGTGGAGAAATGATCCATTTAATTAAAGATTTCGAGTGGATACTGGATACATTTTCTCATTGCATGAGATCACCTACGATTTTTCATGTACTATATCATCTGAATTAGTTCCCATTTACTTTTCAATCTCTACTTGTTAAAAATTTTAGAATAAAGAAACCAATTATGATGCGATCTGGGTCATTACTCTACTATTGGTTGAGGGAGATAGTAAGCATATTATGTAATATCTTTAATATGTTTCTCTTGTAGCCTTGTAAATGTTTTACTAGCTATTTTCACACTAATATTTGTTCTTGCTGAGAGTTCCTTCCTCTTTCCCCTTGTTATTTGGGGTTTAGGAGCCAGTTTGAAGTAGAACTGCTTGATTATGGCCGTCTCAGAATGTTCCATTTGTATAGTTTATTTACTTCATTCCTCAAACCTAATATTGTACTGAGCCAGAGTGTAGTTAGATAGCGGCACTATAGCGCTATTGCATAGTGGAATTTGAGTAAATCGATATTGTTCTGCGATACGCTATTTAGTATTATATTGTGCAATAGTGGCTACAGCGGCACTATAGCGCTGTTGCATTGCAAAATTTCAATAACCCCTATTTTCCAAACTCCGCAATTTATAACAAAGGAGCTGGGTTATTTTTTACCCTTTTTCCTTTTCATTGAAGAGAAAATCTTTGTGCAGTCTCTTAATCAAATAAACTGTAATTTTCTGTGCACATTTGCATTGAAGCTGCCTCTTCTCAATAATAGGAATTATTACTGATTAACTGACTAACGTATATTTATATCAGTTTTCTTTATTTTATTTTCTTATGTTTTCCAATATGACTTATTATTAATTGTATTTCTTCATATCATATCTCAATCATCAGCTGCCCCTCGGTAAAACAAACTAACATGTACAATTTTTTGATCTATTTTAATAAATTTTATCATATATTGTATGCACTTTGATTTCATTTTTCAAATCAATAAAGTGTGCAACTGTGTACCATCTTCAAGGTTTACATATACCTGCAATATAAACTGTTATATAGTTTTTTTATTTTACTGTTATTATGTTCACTGGCATTTATTTAATGCAAGCCTACTTTGTGAATTACATTGAAACTCTACTTGTCGCTTTATTCCACTAGTAATGCATATCTCAAGTGTCAAGACTCTACCAGTTAATCTAATTGTTTTTTTGATTTTGAATGATCTTGACTATACTCTCATTTTTTACCAAGGTTGTTGATTTGATTCTGTTATCAGACCTGAAAATAGCTGCCATTTGTATTTTTAGTCATTTGATAATATATTAATGTCATTCACTAATTTCTGGTTTGTTCATCCATGCAGAAGCATTTGAACAACAGCCAACAAACAACTGGAGGGGGTTCTTTAGTGTCTCATCTTACTCGCAGTATTTCGATGTAGATACAGATGCTGTTATGACCAGATTGATAAGTTCCTTCAATCCAGTTGGTGGAGACTTCTTCAGCAAGATAGATGCTAACCCTGATTTGTGCGTTTTTTTTTTTTTTTTTTTTTTTTATTTGACCTGTGCTTCTATACTTTCAATATTCAAAATAGAGTTGCAAATCTTAATGGGGGGATTTTTTAAGTTTGATCGAACTTACTTTAGCATTTTGTAGGAAATTGCTTGTCATTTTCCTTGTCCACTTATTTTTTATGGATACATGTATTAAGTCATGGGATACATTTACTGAACTTATCCGGGGTTTTAAGGATCATTTCCCAAAATTCATTGATTGTGCATGTTGATGACTATTTATTACTAACTGTATGGTTCTTTTTTATGTTATTGTAGATACGGGCTTATATGGATCTCAACGACATTGGTCTTTGTACTTGCCTCGCTTGGAAATCTTGCCACATTCCTTATGCAACAACATGCAGATAGCGGTACCTCATGGAGCTTTGATGTTAGCTATATGAATACGGCTGCATGGTCTATCTATGGTTATGCAATAGTGGTCCCAATGGCATACTACTTCTTCCTTCAGTACATGGGTTCAAATGCTAACCTTATAAGGTTTTGGTGCATGTGGGGATATTCCCTCTCCATTTTCATCATCTCTTCAGTAAGTTTCTACTTCTTCCTAAGCCCAACAACTGGGATGAATTTTGTTTCTACTTCATCTTATCTACTGACTTCTTTTTGATGTATTTTTGTTTTATCATAAAACATCATGTATTTTAGAAGTTTAGACTTTATTTATATTAGTTGTCATAAGAAATTGCATCATTTGCTAGGTAATTATGCTTTAATTTTGTGTTGCTATCTAATTTATATTACTTGGGATACTTTCAGTTTTTGTTGATAATTCCAGTTGAGATTCTGCGGTGGATCATAACACTCATTACTGGTGTTGCGTCGGCCAGCTTTGTTGCCTTAAACTTGAGGTCTTACATAGAAAGCAATGACATTTCAGTGGCGATCATGGCCGCATTTTTCTTGCAAATAGGTCTGGCAGTCTTCATCAAGGTTTGGTTCTTTGCATAG
Protein sequence

>MS.gene59238.t1

MQIFPPATNGGDRGRGGYQTVGTPTEAFEQQPTNNWRGFFSVSSYSQYFDVDTDAVMTRLISSFNPVGGDFFSKIDANPDLYGLIWISTTLVFVLASLGNLATFLMQQHADSGTSWSFDVSYMNTAAWSIYGYAIVVPMAYYFFLQYMGSNANLIRFWCMWGYSLSIFIISSFLLIIPVEILRWIITLITGVASASFVALNLRSYIESNDISVAIMAAFFLQIGLAVFIKVWFFA