AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59387

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59387.t1 MTR_5g020620 97.326 561 15 0 1 561 1 561 0.0 1140
MS.gene59387.t1 MTR_5g020600 83.837 563 88 3 1 561 5 566 0.0 990
MS.gene59387.t1 MTR_3g462760 63.941 538 193 1 24 561 20 556 0.0 739
MS.gene59387.t1 MTR_5g069680 60.932 558 218 0 4 561 1 558 0.0 736
MS.gene59387.t1 MTR_4g015120 60.107 559 216 4 6 561 3 557 0.0 699
MS.gene59387.t1 MTR_5g081810 58.444 527 218 1 35 561 4 529 0.0 674
MS.gene59387.t1 MTR_7g062310 58.258 551 213 6 27 561 44 593 0.0 662
MS.gene59387.t1 MTR_7g058690 55.190 578 236 9 6 561 4 580 0.0 649
MS.gene59387.t1 MTR_7g458880 54.799 573 234 9 7 561 15 580 0.0 647
MS.gene59387.t1 MTR_7g060460 55.575 583 227 11 6 561 4 581 0.0 645
MS.gene59387.t1 MTR_7g062250 53.633 578 248 8 3 561 2 578 0.0 628
MS.gene59387.t1 MTR_5g073210 53.819 563 245 7 9 561 3 560 0.0 610
MS.gene59387.t1 MTR_5g083360 50.796 565 258 5 13 561 7 567 0.0 608
MS.gene59387.t1 MTR_4g064530 53.025 562 246 9 2 547 14 573 0.0 606
MS.gene59387.t1 MTR_2g008330 49.558 565 273 6 9 561 15 579 0.0 575
MS.gene59387.t1 MTR_4g064530 52.886 537 235 9 2 522 14 548 0.0 570
MS.gene59387.t1 MTR_4g019225 46.812 549 275 4 29 561 25 572 0.0 539
MS.gene59387.t1 MTR_3g071890 49.533 535 243 9 1 517 1 526 0.0 535
MS.gene59387.t1 MTR_3g101640 48.490 563 273 11 13 561 12 571 0.0 524
MS.gene59387.t1 MTR_3g101635 48.551 552 265 9 6 544 4 549 0.0 523
MS.gene59387.t1 MTR_7g065980 45.061 577 288 12 8 561 1 571 1.30e-174 506
MS.gene59387.t1 MTR_7g065970 46.935 571 285 6 8 561 1 570 4.84e-174 504
MS.gene59387.t1 MTR_8g027375 44.505 546 285 6 28 561 32 571 1.38e-172 501
MS.gene59387.t1 MTR_5g081790 52.256 266 125 1 254 519 157 420 1.63e-92 290
MS.gene59387.t1 MTR_5g081790 51.705 176 70 2 4 172 6 173 2.62e-57 198
MS.gene59387.t1 MTR_8g073840 74.286 210 15 3 1 209 1 172 9.14e-92 283
MS.gene59387.t1 MTR_8g091390 32.413 543 318 13 25 539 29 550 8.23e-81 264
MS.gene59387.t1 MTR_3g078700 31.685 546 332 14 6 539 2 518 2.20e-66 225
MS.gene59387.t1 MTR_3g078730 31.734 542 323 17 13 539 8 517 6.62e-66 224
MS.gene59387.t1 MTR_3g078740 31.579 570 308 20 27 539 23 567 1.20e-63 219
MS.gene59387.t1 MTR_3g115050 30.526 570 346 18 1 561 5 533 9.10e-61 210
MS.gene59387.t1 MTR_3g008580 29.809 577 360 18 4 561 1 551 1.22e-59 208
MS.gene59387.t1 MTR_4g075690 29.371 572 353 15 1 561 1 532 8.79e-58 202
MS.gene59387.t1 MTR_1g026410 29.004 562 351 16 10 561 9 532 6.49e-57 200
MS.gene59387.t1 MTR_4g101650 30.000 580 356 20 1 561 1 549 1.22e-55 197
MS.gene59387.t1 MTR_1g090707 28.774 563 351 18 14 561 9 536 3.72e-55 196
MS.gene59387.t1 MTR_4g049390 30.657 548 334 19 23 561 20 530 2.26e-54 192
MS.gene59387.t1 MTR_4g048350 31.496 508 301 20 23 521 20 489 5.01e-51 184
MS.gene59387.t1 MTR_2g088080 30.839 548 333 18 23 561 23 533 1.08e-50 182
MS.gene59387.t1 MTR_8g479390 30.019 513 298 20 25 521 26 493 7.37e-50 181
MS.gene59387.t1 MTR_4g075690 29.835 486 291 14 1 475 1 447 2.88e-49 177
MS.gene59387.t1 MTR_5g018270 29.183 514 306 18 25 525 26 494 5.05e-48 175
MS.gene59387.t1 MTR_4g083210 28.214 560 347 19 10 553 5 525 1.48e-47 174
MS.gene59387.t1 MTR_7g051440 28.824 510 322 16 32 526 31 514 1.98e-47 175
MS.gene59387.t1 MTR_8g479380 28.224 535 314 19 17 527 9 497 1.35e-46 171
MS.gene59387.t1 MTR_3g083400 27.971 547 348 18 9 543 5 517 1.98e-45 168
MS.gene59387.t1 MTR_3g083440 27.848 553 341 20 9 543 5 517 2.15e-45 168
MS.gene59387.t1 MTR_3g083430 27.848 553 341 20 9 543 5 517 2.26e-45 168
MS.gene59387.t1 MTR_3g083390 27.469 557 338 20 9 543 5 517 1.05e-44 166
MS.gene59387.t1 MTR_3g083450 27.860 542 334 19 20 543 16 518 1.11e-44 166
MS.gene59387.t1 MTR_4g132320 30.841 535 315 18 1 521 1 494 1.22e-44 166
MS.gene59387.t1 MTR_3g083410 28.119 505 318 17 51 543 10 481 3.22e-42 159
MS.gene59387.t1 MTR_2g088080 31.288 489 290 18 82 561 1 452 4.10e-41 154
MS.gene59387.t1 MTR_4g083190 28.277 534 326 21 13 527 8 503 3.03e-40 154
MS.gene59387.t1 MTR_7g058670 58.025 81 33 1 87 166 13 93 3.05e-30 114
MS.gene59387.t1 MTR_4g133150 28.796 191 114 6 132 312 19 197 1.24e-13 70.5
MS.gene59387.t1 MTR_3g079660 50.000 60 30 0 471 530 5 64 1.39e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59387.t1 AT5G03260 75.949 553 128 1 14 561 5 557 0.0 882
MS.gene59387.t1 AT2G38080 61.071 560 214 3 4 561 1 558 0.0 726
MS.gene59387.t1 AT5G58910 58.214 560 227 4 8 561 9 567 0.0 699
MS.gene59387.t1 AT5G58910 58.214 560 226 5 8 561 9 566 0.0 695
MS.gene59387.t1 AT5G58910 59.623 530 201 4 38 561 1 523 0.0 668
MS.gene59387.t1 AT5G01190 58.333 552 226 3 13 561 8 558 0.0 665
MS.gene59387.t1 AT5G60020 55.363 578 230 8 10 561 2 577 0.0 650
MS.gene59387.t1 AT5G01190 57.428 552 217 4 13 561 8 544 0.0 642
MS.gene59387.t1 AT5G60020 57.038 547 210 7 38 561 1 545 0.0 641
MS.gene59387.t1 AT2G29130 53.684 570 245 8 10 561 5 573 0.0 635
MS.gene59387.t1 AT5G05390 52.228 561 251 7 14 561 9 565 0.0 605
MS.gene59387.t1 AT1G18140 52.236 559 246 8 24 561 23 581 0.0 605
MS.gene59387.t1 AT2G30210 51.220 574 254 7 3 561 8 570 0.0 600
MS.gene59387.t1 AT5G60020 54.664 536 215 8 10 519 2 535 0.0 588
MS.gene59387.t1 AT2G40370 48.547 585 272 7 1 561 1 580 0.0 577
MS.gene59387.t1 AT5G09360 49.910 557 264 8 14 561 19 569 0.0 570
MS.gene59387.t1 AT5G07130 50.441 567 259 8 13 561 7 569 0.0 565
MS.gene59387.t1 AT3G09220 46.939 539 276 5 16 544 12 550 0.0 523
MS.gene59387.t1 AT5G01040 45.043 575 293 8 1 561 1 566 6.17e-178 515
MS.gene59387.t1 AT2G46570 45.487 565 287 10 11 561 12 569 1.86e-176 510
MS.gene59387.t1 AT5G01050 44.097 576 299 8 1 561 1 568 1.03e-174 506
MS.gene59387.t1 AT5G07130 51.745 487 213 8 93 561 2 484 6.03e-170 491
MS.gene59387.t1 AT5G07130 51.745 487 213 8 93 561 2 484 6.03e-170 491
MS.gene59387.t1 AT5G48100 45.242 557 282 13 8 547 1 551 2.19e-161 472
MS.gene59387.t1 AT3G09220 48.009 452 225 5 103 544 2 453 2.66e-150 440
MS.gene59387.t1 AT2G46570 45.082 488 248 9 87 561 4 484 6.77e-150 439
MS.gene59387.t1 AT4G39830 31.500 600 326 22 11 561 14 577 2.21e-82 268
MS.gene59387.t1 AT5G21105 33.096 562 317 19 13 539 21 558 1.21e-77 256
MS.gene59387.t1 AT5G21100 31.616 563 326 18 24 553 18 554 6.99e-73 243
MS.gene59387.t1 AT5G21105 32.472 542 307 19 13 519 21 538 7.94e-69 232
MS.gene59387.t1 AT4G39830 31.311 511 270 20 96 561 8 482 8.41e-60 206
MS.gene59387.t1 AT4G39830 31.311 511 270 20 96 561 6 480 9.30e-60 206
MS.gene59387.t1 AT4G25240 30.743 579 354 21 1 561 1 550 4.75e-59 206
MS.gene59387.t1 AT4G25240 30.743 579 354 21 1 561 1 550 4.75e-59 206
MS.gene59387.t1 AT1G21860 30.286 525 310 16 16 527 15 496 5.88e-59 205
MS.gene59387.t1 AT1G76160 29.174 569 356 15 1 561 1 530 5.63e-58 202
MS.gene59387.t1 AT1G21850 29.944 531 317 17 9 527 9 496 1.22e-57 202
MS.gene59387.t1 AT4G12420 28.796 573 363 19 8 561 1 547 1.22e-56 200
MS.gene59387.t1 AT4G12420 28.796 573 363 19 8 561 1 547 1.22e-56 200
MS.gene59387.t1 AT4G39830 30.588 510 262 19 96 561 8 469 3.42e-56 196
MS.gene59387.t1 AT1G75790 31.407 519 311 16 14 521 11 495 5.09e-56 197
MS.gene59387.t1 AT5G66920 29.009 555 336 16 21 561 28 538 1.65e-55 196
MS.gene59387.t1 AT5G51480 28.520 554 341 20 31 561 29 550 8.91e-55 195
MS.gene59387.t1 AT1G41830 28.371 571 358 17 1 561 2 531 6.52e-54 192
MS.gene59387.t1 AT4G22010 29.525 569 348 18 5 561 3 530 1.91e-52 187
MS.gene59387.t1 AT5G48450 28.974 497 320 14 32 521 31 501 1.04e-51 186
MS.gene59387.t1 AT5G48450 28.974 497 320 14 32 521 31 501 2.74e-51 186
MS.gene59387.t1 AT4G28090 27.619 525 327 16 51 561 49 534 1.06e-49 180
MS.gene59387.t1 AT4G38420 28.491 530 315 16 14 521 12 499 1.62e-48 177
MS.gene59387.t1 AT2G23630 29.446 523 318 17 50 561 55 537 3.75e-45 167
MS.gene59387.t1 AT2G23630 29.446 523 318 17 50 561 50 532 4.04e-45 167
MS.gene59387.t1 AT4G38420 28.125 512 304 16 14 503 12 481 7.43e-44 163
MS.gene59387.t1 AT5G21105 34.639 332 195 9 215 539 51 367 1.87e-43 160
MS.gene59387.t1 AT4G37160 26.297 559 360 17 15 561 16 534 6.61e-43 161
MS.gene59387.t1 AT1G55560 28.281 541 329 21 32 558 28 523 3.64e-41 156
MS.gene59387.t1 AT4G38420 27.686 484 287 15 14 475 12 454 3.40e-40 152
MS.gene59387.t1 AT1G55570 28.690 481 305 16 50 521 49 500 2.33e-39 151
MS.gene59387.t1 AT3G13400 28.185 518 320 20 19 521 17 497 4.46e-38 148
MS.gene59387.t1 AT3G13400 28.185 518 320 20 19 521 17 497 4.46e-38 148
MS.gene59387.t1 AT3G13390 27.875 513 300 20 34 521 32 499 1.24e-37 146
MS.gene59387.t1 AT4G37160 26.625 477 291 18 15 475 16 449 2.77e-33 132

Find 165 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAAAAGATACATTGTTTA+TGG 0.117825 5.4:-12559302 None:intergenic
AAACCCATTGTAACTGTAAA+TGG 0.125781 5.4:+12557741 MS.gene59387:CDS
TGGCTGAGGGCCACTGTCTA+TGG 0.136642 5.4:+12558103 MS.gene59387:CDS
ATCAAACTGGTATTTCTTTA+TGG 0.153957 5.4:-12557588 None:intergenic
ACAGCTTTCCTTGTTGAAAA+TGG 0.188076 5.4:+12559862 MS.gene59387:CDS
AGGATCTACCAAGTTGTATT+TGG 0.231258 5.4:-12559620 None:intergenic
AGAGAATTTGAAATTGTCTT+AGG 0.257876 5.4:+12558181 MS.gene59387:CDS
ACTTACAAATTCAACGTTAC+TGG 0.262993 5.4:+12558043 MS.gene59387:CDS
TTTCTCTTATTTCTCTTTGT+TGG 0.268775 5.4:+12557542 MS.gene59387:CDS
TGAAAAGCAAGGAAACAAAA+TGG 0.292112 5.4:+12558342 MS.gene59387:CDS
TTTGGAGCTGCACACTGGTT+GGG 0.293072 5.4:+12559831 MS.gene59387:CDS
AGGATAGGTAGGACAGAGTT+TGG 0.301795 5.4:-12559094 None:intergenic
TTGGACCGCAATTCGCTTCC+AGG 0.317781 5.4:+12559678 MS.gene59387:CDS
GAGGCAAGATTGGCGGTTAA+TGG 0.319568 5.4:-12559430 None:intergenic
ACTGTAAATGGGAGGTTTCC+AGG 0.332526 5.4:+12557753 MS.gene59387:CDS
TACTGTTGAGCTAGTGTTGC+AGG 0.335732 5.4:+12559492 MS.gene59387:CDS
CAAAGAAAAGTTCGTCGTTA+AGG 0.342776 5.4:-12558850 None:intergenic
GTATGTGCATCTGACATATT+TGG 0.346152 5.4:-12558373 None:intergenic
GGAAATGGTGTTCCAAGTTT+AGG 0.347068 5.4:-12558142 None:intergenic
GGCTGAGGGCCACTGTCTAT+GGG 0.363346 5.4:+12558104 MS.gene59387:CDS
TTGGAGCTGCACACTGGTTG+GGG 0.365693 5.4:+12559832 MS.gene59387:CDS
TTGGTTCATGCATTGTCATT+TGG 0.381373 5.4:+12559813 MS.gene59387:CDS
TCACATCCATTCCATCTTCA+TGG 0.382480 5.4:+12559538 MS.gene59387:CDS
TATTTGGCTGGGTCTTTAGC+AGG 0.383699 5.4:-12559604 None:intergenic
TGGTCAAACCACAAACGTTC+TGG 0.387286 5.4:+12558958 MS.gene59387:CDS
TATACAATTGCACAGGTGTT+TGG 0.388641 5.4:+12559794 MS.gene59387:intron
TTCGTTGTTGGAAATGGTAT+TGG 0.391745 5.4:+12559571 MS.gene59387:CDS
GAAAAGCAAGGAAACAAAAT+GGG 0.398688 5.4:+12558343 MS.gene59387:CDS
CCAACTGTGTTCCTTTCAAT+AGG 0.402563 5.4:-12559640 None:intergenic
AAAGAAAAGTTCGTCGTTAA+GGG 0.404082 5.4:-12558849 None:intergenic
CCGGTATAGTTGAAAGGAAT+TGG 0.404144 5.4:-12559403 None:intergenic
AATACAACATGTCGATTCAT+TGG 0.410485 5.4:+12557835 MS.gene59387:CDS
TCTACCAAGTTGTATTTGGC+TGG 0.414320 5.4:-12559616 None:intergenic
GCCTGTCAAAGAGGCAAGAT+TGG 0.416849 5.4:-12559440 None:intergenic
CTATTGAAAGGAACACAGTT+GGG 0.417503 5.4:+12559641 MS.gene59387:CDS
TATAATAACTCACATCAAAC+TGG 0.420106 5.4:-12557601 None:intergenic
TAGCTGTTTCCAGTCTGTAT+TGG 0.420779 5.4:-12558019 None:intergenic
GTTCATGCCAACCAAGTTGC+CGG 0.422586 5.4:+12558980 MS.gene59387:CDS
ACTTTGAGAGGAACGTTAGC+AGG 0.431917 5.4:-12559199 None:intergenic
GCCAATCTTGCCTCTTTGAC+AGG 0.432874 5.4:+12559439 MS.gene59387:CDS
ATTTGGAGCTGCACACTGGT+TGG 0.433682 5.4:+12559830 MS.gene59387:CDS
AAAGGAATTGGTGGGCGGTC+AGG 0.434738 5.4:-12559391 None:intergenic
GCAGAACATAATGGCAAGCT+TGG 0.440078 5.4:+12557496 None:intergenic
CTAAACCAATAGTGTAAAAG+AGG 0.440377 5.4:-12559231 None:intergenic
CTCCCATTTACAGTTACAAT+GGG 0.446996 5.4:-12557744 None:intergenic
AGAACCGATTGACCTTGGTT+TGG 0.455032 5.4:-12559886 None:intergenic
ATCGGTTCTGCCACCACCTA+AGG 0.457995 5.4:+12559900 MS.gene59387:CDS
TAGCTCAACAGTAGAGTTGA+AGG 0.463131 5.4:-12559482 None:intergenic
TAGCATGAAGGAAGGTCCTT+AGG 0.463497 5.4:-12559916 None:intergenic
GGGCAGCGTTGATGATTCTC+AGG 0.464968 5.4:-12558829 None:intergenic
ACAATTTCAAATTCTCTAGC+TGG 0.466433 5.4:-12558175 None:intergenic
GGATAGGTAGGACAGAGTTT+GGG 0.468282 5.4:-12559093 None:intergenic
TCAGATGCACATACAATCAA+TGG 0.468699 5.4:+12558382 MS.gene59387:CDS
TATCGTAATGGTTGGGCGGA+CGG 0.469591 5.4:+12557974 MS.gene59387:CDS
CTTACAAATTCAACGTTACT+GGG 0.471978 5.4:+12558044 MS.gene59387:CDS
CTACCAAGTTGTATTTGGCT+GGG 0.473331 5.4:-12559615 None:intergenic
CCTTCCCTAGCATTGATAGT+TGG 0.475485 5.4:-12557777 None:intergenic
TCTCTAGCTGGCTGTGGGAA+AGG 0.477936 5.4:-12558163 None:intergenic
AAAAGTTATATCCATGAAGA+TGG 0.477970 5.4:-12559549 None:intergenic
ATAGTAATCATGCCTAAACT+TGG 0.479188 5.4:+12558130 MS.gene59387:CDS
GGCATGAACCAGAACGTTTG+TGG 0.480039 5.4:-12558966 None:intergenic
CGAAGTACCTACCGGCAACT+TGG 0.481175 5.4:-12558991 None:intergenic
CCAATTCCTTTCAACTATAC+CGG 0.486802 5.4:+12559403 MS.gene59387:CDS
TCAAATTCTCTAGCTGGCTG+TGG 0.491350 5.4:-12558169 None:intergenic
ACATACCTGGATTATCTGCC+TGG 0.497581 5.4:-12559696 None:intergenic
AACCCATTGTAACTGTAAAT+GGG 0.498924 5.4:+12557742 MS.gene59387:CDS
GAACAAGGAAATAGCGGTCC+TGG 0.502785 5.4:-12558409 None:intergenic
TAAACAATATCGTAATGGTT+GGG 0.504714 5.4:+12557967 MS.gene59387:CDS
CCTGGTTCCGTTGAGGCATG+TGG 0.505996 5.4:-12559269 None:intergenic
ATGGATGTGATTCAACTGTT+AGG 0.506413 5.4:-12559525 None:intergenic
AGTATAGCCTGAGTGGTGAA+TGG 0.508417 5.4:-12558926 None:intergenic
CCTCCCATTTACAGTTACAA+TGG 0.508798 5.4:-12557745 None:intergenic
GCTGGCTGTGGGAAAGGAAA+TGG 0.509455 5.4:-12558157 None:intergenic
AGAACGTTTGTGGTTTGACC+AGG 0.510278 5.4:-12558956 None:intergenic
AGCTCACTACTTCAACATCA+AGG 0.511015 5.4:+12559348 MS.gene59387:CDS
TTAAACAATATCGTAATGGT+TGG 0.511801 5.4:+12557966 MS.gene59387:CDS
CCACATGCCTCAACGGAACC+AGG 0.511973 5.4:+12559269 MS.gene59387:CDS
AAACCTGTGTCATTGCTAGC+TGG 0.513434 5.4:-12559127 None:intergenic
TGGACCAAACCAAGGTCAAT+CGG 0.514301 5.4:+12559882 MS.gene59387:CDS
AACACAGTTGGGGTTCCTAC+AGG 0.519624 5.4:+12559652 MS.gene59387:CDS
CAGGGCCAACTATCAATGCT+AGG 0.520051 5.4:+12557772 MS.gene59387:CDS
CAGAACATAATGGCAAGCTT+GGG 0.520370 5.4:+12557497 None:intergenic
TTGGGGTTCCTACAGGTGGT+TGG 0.524926 5.4:+12559659 MS.gene59387:CDS
CTAGCATTGATAGTTGGCCC+TGG 0.525578 5.4:-12557771 None:intergenic
TGCTAGCTGGCAACTGTGGA+AGG 0.527207 5.4:-12559114 None:intergenic
GCTGGCAACTGTGGAAGGAT+AGG 0.531903 5.4:-12559109 None:intergenic
GGCATCCATAAAAGCCTTGG+TGG 0.532942 5.4:-12559017 None:intergenic
GTACCTACCGGCAACTTGGT+TGG 0.533001 5.4:-12558987 None:intergenic
TCCAGACACATTTGCAATGG+AGG 0.537172 5.4:+12558784 MS.gene59387:intron
CGCTTCCAGGCAGATAATCC+AGG 0.538621 5.4:+12559691 MS.gene59387:CDS
CTGGTTCCGTTGAGGCATGT+GGG 0.541062 5.4:-12559268 None:intergenic
GGACTTAAACAATATCGTAA+TGG 0.543784 5.4:+12557962 MS.gene59387:CDS
TTCTGCATGCAATAGGTGAA+TGG 0.543828 5.4:+12558296 MS.gene59387:intron
GTGGCAGAACCGATTGACCT+TGG 0.544566 5.4:-12559891 None:intergenic
CTGTAAATGGGAGGTTTCCA+GGG 0.547833 5.4:+12557754 MS.gene59387:CDS
TGGAGCTGCACACTGGTTGG+GGG 0.549892 5.4:+12559833 MS.gene59387:CDS
TGGGGCATCCATAAAAGCCT+TGG 0.550639 5.4:-12559020 None:intergenic
TACTCTCGTGCCTGTCAAAG+AGG 0.551353 5.4:-12559449 None:intergenic
TTATATCCATGAAGATGGAA+TGG 0.552593 5.4:-12559544 None:intergenic
GTTTATGGAAGCAAGAAACC+TGG 0.553677 5.4:-12559287 None:intergenic
TACTTACGCTTCTCAGAACA+AGG 0.554846 5.4:-12558424 None:intergenic
GGACATTGAGTGATATAAGC+TGG 0.556815 5.4:-12557998 None:intergenic
CAAATTCTCTAGCTGGCTGT+GGG 0.557272 5.4:-12558168 None:intergenic
TACAAAACCATTCACCACTC+AGG 0.561399 5.4:+12558919 MS.gene59387:CDS
GCTGAGGGCCACTGTCTATG+GGG 0.574927 5.4:+12558105 MS.gene59387:CDS
GTGGAAGAGATTGAAAAGCA+AGG 0.575516 5.4:+12558331 MS.gene59387:CDS
ACTCAATGTCCAATACAGAC+TGG 0.577809 5.4:+12558010 MS.gene59387:CDS
CATGTTGTATTGTGCATGGT+TGG 0.578067 5.4:-12557823 None:intergenic
CCTATTGAAAGGAACACAGT+TGG 0.579873 5.4:+12559640 MS.gene59387:CDS
GAAGGAAGGTCCTTAGGTGG+TGG 0.582584 5.4:-12559910 None:intergenic
CAGGCTATACTAATTGCTCC+TGG 0.582951 5.4:+12558938 MS.gene59387:CDS
TGTCATTTGGAGCTGCACAC+TGG 0.584595 5.4:+12559826 MS.gene59387:CDS
AGGTTTCTATCAACTTTGAG+AGG 0.585939 5.4:-12559211 None:intergenic
CTGGGCAGAGAGGAACACTA+TGG 0.589789 5.4:+12558062 MS.gene59387:CDS
TGAACCGATGACTAGCATGA+AGG 0.591968 5.4:-12559928 None:intergenic
TGTCAAAGAGGCAAGATTGG+CGG 0.592555 5.4:-12559437 None:intergenic
TCTTGCCCCACATGCCTCAA+CGG 0.594667 5.4:+12559262 MS.gene59387:CDS
GGCGCACCGGTATAGTTGAA+AGG 0.596639 5.4:-12559409 None:intergenic
ATCTGCCTGGAAGCGAATTG+CGG 0.596877 5.4:-12559683 None:intergenic
ACTTGGTAGATCCTATTGAA+AGG 0.598347 5.4:+12559629 MS.gene59387:CDS
GCAACTGTGGAAGGATAGGT+AGG 0.599848 5.4:-12559105 None:intergenic
AGGGCCAACTATCAATGCTA+GGG 0.601223 5.4:+12557773 MS.gene59387:CDS
AGACCCAGCCAAATACAACT+TGG 0.601571 5.4:+12559612 MS.gene59387:CDS
TGGTTCCGTTGAGGCATGTG+GGG 0.603861 5.4:-12559267 None:intergenic
CTTTGAGAGGAACGTTAGCA+GGG 0.605793 5.4:-12559198 None:intergenic
CATACAATCAATGGAAAACC+AGG 0.607466 5.4:+12558391 MS.gene59387:CDS
TATTGAAAGGAACACAGTTG+GGG 0.607732 5.4:+12559642 MS.gene59387:CDS
TCATTGCTAGCTGGCAACTG+TGG 0.616974 5.4:-12559118 None:intergenic
TATAGTTGAAAGGAATTGGT+GGG 0.618203 5.4:-12559399 None:intergenic
GTATAGTTGAAAGGAATTGG+TGG 0.620277 5.4:-12559400 None:intergenic
CCAACTATCAATGCTAGGGA+AGG 0.622677 5.4:+12557777 MS.gene59387:CDS
GCTCACTACTTCAACATCAA+GGG 0.625835 5.4:+12559349 MS.gene59387:CDS
AGTTGCCGGTAGGTACTTCG+TGG 0.626007 5.4:+12558994 MS.gene59387:CDS
TTTGTTGTCAACTTGAATTG+GGG 0.629530 5.4:-12559038 None:intergenic
TCGACATGTTGTATTGTGCA+TGG 0.631211 5.4:-12557827 None:intergenic
AGCAATTAGTATAGCCTGAG+TGG 0.636864 5.4:-12558933 None:intergenic
ACAGTTGGGGTTCCTACAGG+TGG 0.639766 5.4:+12559655 MS.gene59387:CDS
TTCTCAGAACAAGGAAATAG+CGG 0.640516 5.4:-12558415 None:intergenic
GGTGGCCACGAAGTACCTAC+CGG 0.640930 5.4:-12558999 None:intergenic
TGTCATGTTGTGACCCGCAA+TGG 0.642364 5.4:-12558873 None:intergenic
CAAGAAACCTGGTTCCGTTG+AGG 0.643767 5.4:-12559276 None:intergenic
CCGATGACTAGCATGAAGGA+AGG 0.646571 5.4:-12559924 None:intergenic
ATTGGCGGTTAATGGCGCAC+CGG 0.649826 5.4:-12559422 None:intergenic
GTTGAAAATGGACCAAACCA+AGG 0.653138 5.4:+12559874 MS.gene59387:CDS
CATGAAGGAAGGTCCTTAGG+TGG 0.653405 5.4:-12559913 None:intergenic
TTTGCAATGGAGGTAGAACA+AGG 0.653506 5.4:+12558794 MS.gene59387:CDS
CCATTGTAACTGTAAATGGG+AGG 0.656751 5.4:+12557745 MS.gene59387:CDS
ATTGCGGTCCAACCACCTGT+AGG 0.658953 5.4:-12559667 None:intergenic
TGCATGCAATAGGTGAATGG+TGG 0.661953 5.4:+12558299 MS.gene59387:intron
TACTATTGCCCCATAGACAG+TGG 0.668736 5.4:-12558113 None:intergenic
GCTTGAAGAAGCGCAACTTG+AGG 0.670899 5.4:-12559328 None:intergenic
GGCAGAGAGGAACACTATGG+TGG 0.671860 5.4:+12558065 MS.gene59387:CDS
AAAAGCAAGGAAACAAAATG+GGG 0.672657 5.4:+12558344 MS.gene59387:CDS
ATGCCAACCAAGTTGCCGGT+AGG 0.673121 5.4:+12558984 MS.gene59387:CDS
ACAATATCGTAATGGTTGGG+CGG 0.673866 5.4:+12557970 MS.gene59387:CDS
TGAATGGTGGAACAATGATG+TGG 0.681264 5.4:+12558312 MS.gene59387:CDS
TGTGCATCTGACATATTTGG+TGG 0.682225 5.4:-12558370 None:intergenic
TTGCCAGCTAGCAATGACAC+AGG 0.686241 5.4:+12559124 MS.gene59387:CDS
TCACAACATGACAGTAGTGG+AGG 0.687744 5.4:+12558883 MS.gene59387:CDS
TTCAACGTTACTGGGCAGAG+AGG 0.688473 5.4:+12558052 MS.gene59387:CDS
CAGCGTTGATGATTCTCAGG+AGG 0.689860 5.4:-12558826 None:intergenic
GGGTCACAACATGACAGTAG+TGG 0.698467 5.4:+12558880 MS.gene59387:CDS
TAGGTACTTCGTGGCCACCA+AGG 0.707531 5.4:+12559003 MS.gene59387:CDS
TTGCAATGGAGGTAGAACAA+GGG 0.716920 5.4:+12558795 MS.gene59387:CDS
AGTTGAAAGGAATTGGTGGG+CGG 0.718230 5.4:-12559396 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATATATATGCTTATTAT+AGG + chr5.4:12557936-12557955 MS.gene59387:intron 10.0%
!! CAAATTGATTAAATAAATAT+TGG + chr5.4:12557871-12557890 MS.gene59387:intron 10.0%
!!! TTCTATAATATTTTACTTTA+TGG + chr5.4:12559736-12559755 MS.gene59387:intron 10.0%
!! ATAAAGTAATAACTAAAATC+TGG - chr5.4:12558481-12558500 None:intergenic 15.0%
!!! TCTTTATAAGACTTTTTTTT+TGG - chr5.4:12558231-12558250 None:intergenic 15.0%
!!! TGAAGCAATTTTTTTTTAAA+AGG - chr5.4:12558760-12558779 None:intergenic 15.0%
!! AAAGAACAAAATGCTATTAA+GGG - chr5.4:12557679-12557698 None:intergenic 20.0%
!! GTAAAATATTATAGAAGCTT+AGG - chr5.4:12559732-12559751 None:intergenic 20.0%
!!! AAAGTCTTATAAAGATTCAT+CGG + chr5.4:12558236-12558255 MS.gene59387:intron 20.0%
! AAAAGTTATATCCATGAAGA+TGG - chr5.4:12559552-12559571 None:intergenic 25.0%
! ATATATGCTTATTATAGGCA+TGG + chr5.4:12557941-12557960 MS.gene59387:intron 25.0%
! ATCAAACTGGTATTTCTTTA+TGG - chr5.4:12557591-12557610 None:intergenic 25.0%
! CACAAAAGATACATTGTTTA+TGG - chr5.4:12559305-12559324 None:intergenic 25.0%
! GAAAGAACAAAATGCTATTA+AGG - chr5.4:12557680-12557699 None:intergenic 25.0%
! TAAAATTAGTAGCACAAGTT+TGG + chr5.4:12558445-12558464 MS.gene59387:intron 25.0%
! TAAACAATATCGTAATGGTT+GGG + chr5.4:12557967-12557986 MS.gene59387:CDS 25.0%
! TATAATAACTCACATCAAAC+TGG - chr5.4:12557604-12557623 None:intergenic 25.0%
! TATACTGTAGACCTAATTAA+CGG + chr5.4:12558636-12558655 MS.gene59387:intron 25.0%
! TTAAACAATATCGTAATGGT+TGG + chr5.4:12557966-12557985 MS.gene59387:CDS 25.0%
! TTGCTAGCTATAATAAGTTA+AGG - chr5.4:12557647-12557666 None:intergenic 25.0%
!! AGAGAATTTGAAATTGTCTT+AGG + chr5.4:12558181-12558200 MS.gene59387:CDS 25.0%
!! CAATTATACATACTCTTTTG+TGG - chr5.4:12558507-12558526 None:intergenic 25.0%
!! CACAAAAGAGTATGTATAAT+TGG + chr5.4:12558505-12558524 MS.gene59387:intron 25.0%
!! TTTCTCTTATTTCTCTTTGT+TGG + chr5.4:12557542-12557561 MS.gene59387:CDS 25.0%
!! TTTTGTATATACAATTGCAC+AGG + chr5.4:12559787-12559806 MS.gene59387:intron 25.0%
!!! AGTCTTTTTTTTTAACAAGC+AGG - chr5.4:12558612-12558631 None:intergenic 25.0%
!!! TTTTGTTGTCAACTTGAATT+GGG - chr5.4:12559042-12559061 None:intergenic 25.0%
AAAAGCAAGGAAACAAAATG+GGG + chr5.4:12558344-12558363 MS.gene59387:CDS 30.0%
AAACCCATTGTAACTGTAAA+TGG + chr5.4:12557741-12557760 MS.gene59387:CDS 30.0%
AAAGAAAAGTTCGTCGTTAA+GGG - chr5.4:12558852-12558871 None:intergenic 30.0%
AACCCATTGTAACTGTAAAT+GGG + chr5.4:12557742-12557761 MS.gene59387:CDS 30.0%
ACAATTTCAAATTCTCTAGC+TGG - chr5.4:12558178-12558197 None:intergenic 30.0%
ACTTACAAATTCAACGTTAC+TGG + chr5.4:12558043-12558062 MS.gene59387:CDS 30.0%
ATAGTAATCATGCCTAAACT+TGG + chr5.4:12558130-12558149 MS.gene59387:CDS 30.0%
CTAAACCAATAGTGTAAAAG+AGG - chr5.4:12559234-12559253 None:intergenic 30.0%
CTTACAAATTCAACGTTACT+GGG + chr5.4:12558044-12558063 MS.gene59387:CDS 30.0%
GAAAAGCAAGGAAACAAAAT+GGG + chr5.4:12558343-12558362 MS.gene59387:CDS 30.0%
GGACTTAAACAATATCGTAA+TGG + chr5.4:12557962-12557981 MS.gene59387:CDS 30.0%
TATAGTTGAAAGGAATTGGT+GGG - chr5.4:12559402-12559421 None:intergenic 30.0%
TGAAAAGCAAGGAAACAAAA+TGG + chr5.4:12558342-12558361 MS.gene59387:CDS 30.0%
TTATATCCATGAAGATGGAA+TGG - chr5.4:12559547-12559566 None:intergenic 30.0%
TTATTATAGCTAGCAATGCA+AGG + chr5.4:12557651-12557670 MS.gene59387:intron 30.0%
TTGTATTTCGTCCGTTAATT+AGG - chr5.4:12558650-12558669 None:intergenic 30.0%
! AGAAACCTCTTTTACACTAT+TGG + chr5.4:12559226-12559245 MS.gene59387:CDS 30.0%
! TTTGTTGTCAACTTGAATTG+GGG - chr5.4:12559041-12559060 None:intergenic 30.0%
!! AATACAACATGTCGATTCAT+TGG + chr5.4:12557835-12557854 MS.gene59387:CDS 30.0%
!! GTTTTGTTGTCAACTTGAAT+TGG - chr5.4:12559043-12559062 None:intergenic 30.0%
!! TAATTGTTTCTGCATGCAAT+AGG + chr5.4:12558289-12558308 MS.gene59387:intron 30.0%
!!! AACTTTTTCGTTGTTGGAAA+TGG + chr5.4:12559565-12559584 MS.gene59387:CDS 30.0%
!!! CTCTTTTACACTATTGGTTT+AGG + chr5.4:12559232-12559251 MS.gene59387:CDS 30.0%
!!! GGATATAACTTTTTCGTTGT+TGG + chr5.4:12559559-12559578 MS.gene59387:CDS 30.0%
ACAGCTTTCCTTGTTGAAAA+TGG + chr5.4:12559862-12559881 MS.gene59387:CDS 35.0%
ACTTGGTAGATCCTATTGAA+AGG + chr5.4:12559629-12559648 MS.gene59387:CDS 35.0%
AGGATCTACCAAGTTGTATT+TGG - chr5.4:12559623-12559642 None:intergenic 35.0%
AGGTTTCTATCAACTTTGAG+AGG - chr5.4:12559214-12559233 None:intergenic 35.0%
ATGGATGTGATTCAACTGTT+AGG - chr5.4:12559528-12559547 None:intergenic 35.0%
CAAAGAAAAGTTCGTCGTTA+AGG - chr5.4:12558853-12558872 None:intergenic 35.0%
CATACAATCAATGGAAAACC+AGG + chr5.4:12558391-12558410 MS.gene59387:CDS 35.0%
CCAATTCCTTTCAACTATAC+CGG + chr5.4:12559403-12559422 MS.gene59387:CDS 35.0%
CTATTGAAAGGAACACAGTT+GGG + chr5.4:12559641-12559660 MS.gene59387:CDS 35.0%
CTCCCATTTACAGTTACAAT+GGG - chr5.4:12557747-12557766 None:intergenic 35.0%
GTATAGTTGAAAGGAATTGG+TGG - chr5.4:12559403-12559422 None:intergenic 35.0%
GTATGTGCATCTGACATATT+TGG - chr5.4:12558376-12558395 None:intergenic 35.0%
TATACAATTGCACAGGTGTT+TGG + chr5.4:12559794-12559813 MS.gene59387:intron 35.0%
TATTGAAAGGAACACAGTTG+GGG + chr5.4:12559642-12559661 MS.gene59387:CDS 35.0%
TCAGATGCACATACAATCAA+TGG + chr5.4:12558382-12558401 MS.gene59387:CDS 35.0%
TTCGTTGTTGGAAATGGTAT+TGG + chr5.4:12559571-12559590 MS.gene59387:CDS 35.0%
TTCTCAGAACAAGGAAATAG+CGG - chr5.4:12558418-12558437 None:intergenic 35.0%
TTGGTTCATGCATTGTCATT+TGG + chr5.4:12559813-12559832 MS.gene59387:CDS 35.0%
! TTTTCCAGACACATTTGCAA+TGG + chr5.4:12558781-12558800 MS.gene59387:intron 35.0%
ACAATATCGTAATGGTTGGG+CGG + chr5.4:12557970-12557989 MS.gene59387:CDS 40.0%
ACTCAATGTCCAATACAGAC+TGG + chr5.4:12558010-12558029 MS.gene59387:CDS 40.0%
AGCAATTAGTATAGCCTGAG+TGG - chr5.4:12558936-12558955 None:intergenic 40.0%
AGCTCACTACTTCAACATCA+AGG + chr5.4:12559348-12559367 MS.gene59387:CDS 40.0%
CATGTTGTATTGTGCATGGT+TGG - chr5.4:12557826-12557845 None:intergenic 40.0%
CCAACTGTGTTCCTTTCAAT+AGG - chr5.4:12559643-12559662 None:intergenic 40.0%
CCATTGTAACTGTAAATGGG+AGG + chr5.4:12557745-12557764 MS.gene59387:CDS 40.0%
CCGGTATAGTTGAAAGGAAT+TGG - chr5.4:12559406-12559425 None:intergenic 40.0%
CCTATTGAAAGGAACACAGT+TGG + chr5.4:12559640-12559659 MS.gene59387:CDS 40.0%
CCTCCCATTTACAGTTACAA+TGG - chr5.4:12557748-12557767 None:intergenic 40.0%
CTACCAAGTTGTATTTGGCT+GGG - chr5.4:12559618-12559637 None:intergenic 40.0%
GCTCACTACTTCAACATCAA+GGG + chr5.4:12559349-12559368 MS.gene59387:CDS 40.0%
GGAAATGGTGTTCCAAGTTT+AGG - chr5.4:12558145-12558164 None:intergenic 40.0%
GGACATTGAGTGATATAAGC+TGG - chr5.4:12558001-12558020 None:intergenic 40.0%
GTGGAAGAGATTGAAAAGCA+AGG + chr5.4:12558331-12558350 MS.gene59387:CDS 40.0%
GTTGAAAATGGACCAAACCA+AGG + chr5.4:12559874-12559893 MS.gene59387:CDS 40.0%
GTTTATGGAAGCAAGAAACC+TGG - chr5.4:12559290-12559309 None:intergenic 40.0%
TACAAAACCATTCACCACTC+AGG + chr5.4:12558919-12558938 MS.gene59387:CDS 40.0%
TACTTACGCTTCTCAGAACA+AGG - chr5.4:12558427-12558446 None:intergenic 40.0%
TAGCTCAACAGTAGAGTTGA+AGG - chr5.4:12559485-12559504 None:intergenic 40.0%
TCACATCCATTCCATCTTCA+TGG + chr5.4:12559538-12559557 MS.gene59387:CDS 40.0%
TCGACATGTTGTATTGTGCA+TGG - chr5.4:12557830-12557849 None:intergenic 40.0%
TCTACCAAGTTGTATTTGGC+TGG - chr5.4:12559619-12559638 None:intergenic 40.0%
TGTGCATCTGACATATTTGG+TGG - chr5.4:12558373-12558392 None:intergenic 40.0%
TTCTGCATGCAATAGGTGAA+TGG + chr5.4:12558296-12558315 MS.gene59387:intron 40.0%
TTGCAATGGAGGTAGAACAA+GGG + chr5.4:12558795-12558814 MS.gene59387:CDS 40.0%
TTTGCAATGGAGGTAGAACA+AGG + chr5.4:12558794-12558813 MS.gene59387:CDS 40.0%
! CGAACTTTTCTTTGCCATTG+CGG + chr5.4:12558859-12558878 MS.gene59387:CDS 40.0%
! GAACTTTTCTTTGCCATTGC+GGG + chr5.4:12558860-12558879 MS.gene59387:CDS 40.0%
! TAGCTGTTTCCAGTCTGTAT+TGG - chr5.4:12558022-12558041 None:intergenic 40.0%
! TGAATGGTGGAACAATGATG+TGG + chr5.4:12558312-12558331 MS.gene59387:CDS 40.0%
!! CAGTTACAATGGGTTTTGCA+TGG - chr5.4:12557737-12557756 None:intergenic 40.0%
!! GGTTTGGTCCATTTTCAACA+AGG - chr5.4:12559873-12559892 None:intergenic 40.0%
ACATACCTGGATTATCTGCC+TGG - chr5.4:12559699-12559718 None:intergenic 45.0%
ACCTCCATTGCAAATGTGTC+TGG - chr5.4:12558788-12558807 None:intergenic 45.0%
ACTGTAAATGGGAGGTTTCC+AGG + chr5.4:12557753-12557772 MS.gene59387:CDS 45.0%
AGAACCGATTGACCTTGGTT+TGG - chr5.4:12559889-12559908 None:intergenic 45.0%
AGAACGTTTGTGGTTTGACC+AGG - chr5.4:12558959-12558978 None:intergenic 45.0%
AGACCCAGCCAAATACAACT+TGG + chr5.4:12559612-12559631 MS.gene59387:CDS 45.0%
AGGACAACTTCAGACATACC+TGG - chr5.4:12559712-12559731 None:intergenic 45.0%
AGGATAGGTAGGACAGAGTT+TGG - chr5.4:12559097-12559116 None:intergenic 45.0%
AGGGCCAACTATCAATGCTA+GGG + chr5.4:12557773-12557792 MS.gene59387:CDS 45.0%
AGTATAGCCTGAGTGGTGAA+TGG - chr5.4:12558929-12558948 None:intergenic 45.0%
AGTTGAAAGGAATTGGTGGG+CGG - chr5.4:12559399-12559418 None:intergenic 45.0%
CAAATTCTCTAGCTGGCTGT+GGG - chr5.4:12558171-12558190 None:intergenic 45.0%
CAGGCTATACTAATTGCTCC+TGG + chr5.4:12558938-12558957 MS.gene59387:CDS 45.0%
CCAACTATCAATGCTAGGGA+AGG + chr5.4:12557777-12557796 MS.gene59387:CDS 45.0%
CTGTAAATGGGAGGTTTCCA+GGG + chr5.4:12557754-12557773 MS.gene59387:CDS 45.0%
GGATAGGTAGGACAGAGTTT+GGG - chr5.4:12559096-12559115 None:intergenic 45.0%
TACTATTGCCCCATAGACAG+TGG - chr5.4:12558116-12558135 None:intergenic 45.0%
TACTGTTGAGCTAGTGTTGC+AGG + chr5.4:12559492-12559511 MS.gene59387:CDS 45.0%
TAGCATGAAGGAAGGTCCTT+AGG - chr5.4:12559919-12559938 None:intergenic 45.0%
TATTTGGCTGGGTCTTTAGC+AGG - chr5.4:12559607-12559626 None:intergenic 45.0%
TCAAATTCTCTAGCTGGCTG+TGG - chr5.4:12558172-12558191 None:intergenic 45.0%
TCACAACATGACAGTAGTGG+AGG + chr5.4:12558883-12558902 MS.gene59387:CDS 45.0%
TCCAGACACATTTGCAATGG+AGG + chr5.4:12558784-12558803 MS.gene59387:intron 45.0%
TGCATGCAATAGGTGAATGG+TGG + chr5.4:12558299-12558318 MS.gene59387:intron 45.0%
TGGACCAAACCAAGGTCAAT+CGG + chr5.4:12559882-12559901 MS.gene59387:CDS 45.0%
TGGTCAAACCACAAACGTTC+TGG + chr5.4:12558958-12558977 MS.gene59387:CDS 45.0%
TGTCAAAGAGGCAAGATTGG+CGG - chr5.4:12559440-12559459 None:intergenic 45.0%
! AAACCTGTGTCATTGCTAGC+TGG - chr5.4:12559130-12559149 None:intergenic 45.0%
! ACTTTGAGAGGAACGTTAGC+AGG - chr5.4:12559202-12559221 None:intergenic 45.0%
! CTTTGAGAGGAACGTTAGCA+GGG - chr5.4:12559201-12559220 None:intergenic 45.0%
!! CCTTCCCTAGCATTGATAGT+TGG - chr5.4:12557780-12557799 None:intergenic 45.0%
AACACAGTTGGGGTTCCTAC+AGG + chr5.4:12559652-12559671 MS.gene59387:CDS 50.0%
ATCTGCCTGGAAGCGAATTG+CGG - chr5.4:12559686-12559705 None:intergenic 50.0%
CAAGAAACCTGGTTCCGTTG+AGG - chr5.4:12559279-12559298 None:intergenic 50.0%
CAGGGCCAACTATCAATGCT+AGG + chr5.4:12557772-12557791 MS.gene59387:CDS 50.0%
CATGAAGGAAGGTCCTTAGG+TGG - chr5.4:12559916-12559935 None:intergenic 50.0%
GAACAAGGAAATAGCGGTCC+TGG - chr5.4:12558412-12558431 None:intergenic 50.0%
GAGGCAAGATTGGCGGTTAA+TGG - chr5.4:12559433-12559452 None:intergenic 50.0%
GCAACTGTGGAAGGATAGGT+AGG - chr5.4:12559108-12559127 None:intergenic 50.0%
GCCAATCTTGCCTCTTTGAC+AGG + chr5.4:12559439-12559458 MS.gene59387:CDS 50.0%
GCCTGTCAAAGAGGCAAGAT+TGG - chr5.4:12559443-12559462 None:intergenic 50.0%
GGCATCCATAAAAGCCTTGG+TGG - chr5.4:12559020-12559039 None:intergenic 50.0%
GGCATGAACCAGAACGTTTG+TGG - chr5.4:12558969-12558988 None:intergenic 50.0%
GGGTCACAACATGACAGTAG+TGG + chr5.4:12558880-12558899 MS.gene59387:CDS 50.0%
GTTCATGCCAACCAAGTTGC+CGG + chr5.4:12558980-12558999 MS.gene59387:CDS 50.0%
TACTCTCGTGCCTGTCAAAG+AGG - chr5.4:12559452-12559471 None:intergenic 50.0%
TATCGTAATGGTTGGGCGGA+CGG + chr5.4:12557974-12557993 MS.gene59387:CDS 50.0%
TCATTGCTAGCTGGCAACTG+TGG - chr5.4:12559121-12559140 None:intergenic 50.0%
TGTCATGTTGTGACCCGCAA+TGG - chr5.4:12558876-12558895 None:intergenic 50.0%
TTCAACGTTACTGGGCAGAG+AGG + chr5.4:12558052-12558071 MS.gene59387:CDS 50.0%
TTGCCAGCTAGCAATGACAC+AGG + chr5.4:12559124-12559143 MS.gene59387:CDS 50.0%
! ATTTGGAGCTGCACACTGGT+TGG + chr5.4:12559830-12559849 MS.gene59387:CDS 50.0%
! GCTTGAAGAAGCGCAACTTG+AGG - chr5.4:12559331-12559350 None:intergenic 50.0%
! TGGGGCATCCATAAAAGCCT+TGG - chr5.4:12559023-12559042 None:intergenic 50.0%
! TGTCATTTGGAGCTGCACAC+TGG + chr5.4:12559826-12559845 MS.gene59387:CDS 50.0%
! TTTGGAGCTGCACACTGGTT+GGG + chr5.4:12559831-12559850 MS.gene59387:CDS 50.0%
!! CAGCGTTGATGATTCTCAGG+AGG - chr5.4:12558829-12558848 None:intergenic 50.0%
!! CTAGCATTGATAGTTGGCCC+TGG - chr5.4:12557774-12557793 None:intergenic 50.0%
!!! ACGCACACATTTTCTGGCTG+AGG + chr5.4:12558089-12558108 MS.gene59387:CDS 50.0%
!!! CGCACACATTTTCTGGCTGA+GGG + chr5.4:12558090-12558109 MS.gene59387:CDS 50.0%
ACAGTTGGGGTTCCTACAGG+TGG + chr5.4:12559655-12559674 MS.gene59387:CDS 55.0%
AGTTGCCGGTAGGTACTTCG+TGG + chr5.4:12558994-12559013 MS.gene59387:CDS 55.0%
ATGCCAACCAAGTTGCCGGT+AGG + chr5.4:12558984-12559003 MS.gene59387:CDS 55.0%
ATTGCGGTCCAACCACCTGT+AGG - chr5.4:12559670-12559689 None:intergenic 55.0%
ATTGGCGGTTAATGGCGCAC+CGG - chr5.4:12559425-12559444 None:intergenic 55.0%
CGCTTCCAGGCAGATAATCC+AGG + chr5.4:12559691-12559710 MS.gene59387:CDS 55.0%
CTGGGCAGAGAGGAACACTA+TGG + chr5.4:12558062-12558081 MS.gene59387:CDS 55.0%
CTGGTTCCGTTGAGGCATGT+GGG - chr5.4:12559271-12559290 None:intergenic 55.0%
GAAGGAAGGTCCTTAGGTGG+TGG - chr5.4:12559913-12559932 None:intergenic 55.0%
GCTGGCAACTGTGGAAGGAT+AGG - chr5.4:12559112-12559131 None:intergenic 55.0%
GCTGGCTGTGGGAAAGGAAA+TGG - chr5.4:12558160-12558179 None:intergenic 55.0%
GGCAGAGAGGAACACTATGG+TGG + chr5.4:12558065-12558084 MS.gene59387:CDS 55.0%
GTACCTACCGGCAACTTGGT+TGG - chr5.4:12558990-12559009 None:intergenic 55.0%
GTGGCAGAACCGATTGACCT+TGG - chr5.4:12559894-12559913 None:intergenic 55.0%
TAGGTACTTCGTGGCCACCA+AGG + chr5.4:12559003-12559022 MS.gene59387:CDS 55.0%
TCTCTAGCTGGCTGTGGGAA+AGG - chr5.4:12558166-12558185 None:intergenic 55.0%
TCTTGCCCCACATGCCTCAA+CGG + chr5.4:12559262-12559281 MS.gene59387:CDS 55.0%
TGCTAGCTGGCAACTGTGGA+AGG - chr5.4:12559117-12559136 None:intergenic 55.0%
TGGTTCCGTTGAGGCATGTG+GGG - chr5.4:12559270-12559289 None:intergenic 55.0%
TTGGACCGCAATTCGCTTCC+AGG + chr5.4:12559678-12559697 MS.gene59387:CDS 55.0%
TTGGGGTTCCTACAGGTGGT+TGG + chr5.4:12559659-12559678 MS.gene59387:CDS 55.0%
! AAAGGAATTGGTGGGCGGTC+AGG - chr5.4:12559394-12559413 None:intergenic 55.0%
! ATCGGTTCTGCCACCACCTA+AGG + chr5.4:12559900-12559919 MS.gene59387:CDS 55.0%
! CGAAGTACCTACCGGCAACT+TGG - chr5.4:12558994-12559013 None:intergenic 55.0%
! CGTGGCCACCAAGGCTTTTA+TGG + chr5.4:12559012-12559031 MS.gene59387:CDS 55.0%
! GGCGCACCGGTATAGTTGAA+AGG - chr5.4:12559412-12559431 None:intergenic 55.0%
! GGTGGCACGCACACATTTTC+TGG + chr5.4:12558083-12558102 MS.gene59387:CDS 55.0%
! TTGGAGCTGCACACTGGTTG+GGG + chr5.4:12559832-12559851 MS.gene59387:CDS 55.0%
!! GGGCAGCGTTGATGATTCTC+AGG - chr5.4:12558832-12558851 None:intergenic 55.0%
CCACATGCCTCAACGGAACC+AGG + chr5.4:12559269-12559288 MS.gene59387:CDS 60.0%
CCTGGTTCCGTTGAGGCATG+TGG - chr5.4:12559272-12559291 None:intergenic 60.0%
GCTGAGGGCCACTGTCTATG+GGG + chr5.4:12558105-12558124 MS.gene59387:CDS 60.0%
GGCTGAGGGCCACTGTCTAT+GGG + chr5.4:12558104-12558123 MS.gene59387:CDS 60.0%
TGGCTGAGGGCCACTGTCTA+TGG + chr5.4:12558103-12558122 MS.gene59387:CDS 60.0%
! GGTGGCCACGAAGTACCTAC+CGG - chr5.4:12559002-12559021 None:intergenic 60.0%
! TGGAGCTGCACACTGGTTGG+GGG + chr5.4:12559833-12559852 MS.gene59387:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 12557506 12559939 12557506 ID=MS.gene59387
chr5.4 mRNA 12557506 12559939 12557506 ID=MS.gene59387.t1;Parent=MS.gene59387
chr5.4 exon 12557506 12557610 12557506 ID=MS.gene59387.t1.exon1;Parent=MS.gene59387.t1
chr5.4 CDS 12557506 12557610 12557506 ID=cds.MS.gene59387.t1;Parent=MS.gene59387.t1
chr5.4 exon 12557705 12557856 12557705 ID=MS.gene59387.t1.exon2;Parent=MS.gene59387.t1
chr5.4 CDS 12557705 12557856 12557705 ID=cds.MS.gene59387.t1;Parent=MS.gene59387.t1
chr5.4 exon 12557958 12558202 12557958 ID=MS.gene59387.t1.exon3;Parent=MS.gene59387.t1
chr5.4 CDS 12557958 12558202 12557958 ID=cds.MS.gene59387.t1;Parent=MS.gene59387.t1
chr5.4 exon 12558311 12558439 12558311 ID=MS.gene59387.t1.exon4;Parent=MS.gene59387.t1
chr5.4 CDS 12558311 12558439 12558311 ID=cds.MS.gene59387.t1;Parent=MS.gene59387.t1
chr5.4 exon 12558789 12559712 12558789 ID=MS.gene59387.t1.exon5;Parent=MS.gene59387.t1
chr5.4 CDS 12558789 12559712 12558789 ID=cds.MS.gene59387.t1;Parent=MS.gene59387.t1
chr5.4 exon 12559809 12559939 12559809 ID=MS.gene59387.t1.exon6;Parent=MS.gene59387.t1
chr5.4 CDS 12559809 12559939 12559809 ID=cds.MS.gene59387.t1;Parent=MS.gene59387.t1
Gene Sequence

>MS.gene59387

ATGGCAAGCTTGGGAAGTTTCATGTCTGCATTAAACTTTCTCTTATTTCTCTTTGTTGGTTTAATCTCTTCATCTGAAGCTGCCATAAAGAAATACCAGTTTGATGTGAGTTATTATATTTTGATCATATTCAGCATACCTTAACTTATTATAGCTAGCAATGCAAGGTTCCCTTAATAGCATTTTGTTCTTTCTACAGATTCAAGTGAAGAATGTGAGTAGACTGTGCCATGCAAAACCCATTGTAACTGTAAATGGGAGGTTTCCAGGGCCAACTATCAATGCTAGGGAAGGAGATCAAGTGCTTGTCAACGTTACCAACCATGCACAATACAACATGTCGATTCATTGGTAGCTGAATTTCACAAATTGATTAAATAAATATTGGTGAAGTTTTTAACACTTGAAAATACATGTTTTTTCTCTAACAATTATATATATGCTTATTATAGGCATGGACTTAAACAATATCGTAATGGTTGGGCGGACGGACCAGCTTATATCACTCAATGTCCAATACAGACTGGAAACAGCTATACTTACAAATTCAACGTTACTGGGCAGAGAGGAACACTATGGTGGCACGCACACATTTTCTGGCTGAGGGCCACTGTCTATGGGGCAATAGTAATCATGCCTAAACTTGGAACACCATTTCCTTTCCCACAGCCAGCTAGAGAATTTGAAATTGTCTTAGGTAAGTATTATAAAGATTCATTTTTCCAAAAAAAAAGTCTTATAAAGATTCATCGGATGAAATAAGAAGTATGAAAGTTGATTTACTAATTGTTTCTGCATGCAATAGGTGAATGGTGGAACAATGATGTGGAAGAGATTGAAAAGCAAGGAAACAAAATGGGGTTGCCACCAAATATGTCAGATGCACATACAATCAATGGAAAACCAGGACCGCTATTTCCTTGTTCTGAGAAGCGTAAGTAAAATTAGTAGCACAAGTTTGGTAACAACTAACCAGATTTTAGTTATTACTTTATAGTCCACAAAAGAGTATGTATAATTGGAGAGTAATTTTTTGAATATTATTTCAAAAATCAAAAGTAATGAGAATAGAATCGCTAAACAGTTTAAATAGACTATGCTATCCTGCTTGTTAAAAAAAAAGACTATGCTATACTGTAGACCTAATTAACGGACGAAATACAATTTGTACAAATAATAACAAAATATATAATTATATACACAAAAGAGTGAAATCTTTGTTTGAAGTTCAATTAATTTGCTTCAATTTTTCCTTTTAAAAAAAAATTGCTTCAATTTTCCAGACACATTTGCAATGGAGGTAGAACAAGGGAAGACATACCTCCTGAGAATCATCAACGCTGCCCTTAACGACGAACTTTTCTTTGCCATTGCGGGTCACAACATGACAGTAGTGGAGGTTGATGCTGTTTATACAAAACCATTCACCACTCAGGCTATACTAATTGCTCCTGGTCAAACCACAAACGTTCTGGTTCATGCCAACCAAGTTGCCGGTAGGTACTTCGTGGCCACCAAGGCTTTTATGGATGCCCCAATTCAAGTTGACAACAAAACTGCTACAGCTATACTTCAATACAAAGACATCCCAAACTCTGTCCTACCTATCCTTCCACAGTTGCCAGCTAGCAATGACACAGGTTTTGCTTTGAGTTACAACAAAAAGATCAGAAGCTTGAACTCTGCTAAGTACCCTGCTAACGTTCCTCTCAAAGTTGATAGAAACCTCTTTTACACTATTGGTTTAGGCAAAAATTCTTGCCCCACATGCCTCAACGGAACCAGGTTTCTTGCTTCCATAAACAATGTATCTTTTGTGATGCCTCAAGTTGCGCTTCTTCAAGCTCACTACTTCAACATCAAGGGTGTTTTCAGAGCTGATTTTCCTGACCGCCCACCAATTCCTTTCAACTATACCGGTGCGCCATTAACCGCCAATCTTGCCTCTTTGACAGGCACGAGAGTAAACAAGATTGCCTTCAACTCTACTGTTGAGCTAGTGTTGCAGGATACTAATCTCCTAACAGTTGAATCACATCCATTCCATCTTCATGGATATAACTTTTTCGTTGTTGGAAATGGTATTGGTAATTTTGATCCTGCTAAAGACCCAGCCAAATACAACTTGGTAGATCCTATTGAAAGGAACACAGTTGGGGTTCCTACAGGTGGTTGGACCGCAATTCGCTTCCAGGCAGATAATCCAGGTATGTCTGAAGTTGTCCTAAGCTTCTATAATATTTTACTTTATGGCATATTCTGCTATGTCACGACATGATGTTTTTGTATATACAATTGCACAGGTGTTTGGTTCATGCATTGTCATTTGGAGCTGCACACTGGTTGGGGGTTGAAAACAGCTTTCCTTGTTGAAAATGGACCAAACCAAGGTCAATCGGTTCTGCCACCACCTAAGGACCTTCCTTCATGCTAG
Protein sequence

>MS.gene59387.t1

MASLGSFMSALNFLLFLFVGLISSSEAAIKKYQFDIQVKNVSRLCHAKPIVTVNGRFPGPTINAREGDQVLVNVTNHAQYNMSIHWHGLKQYRNGWADGPAYITQCPIQTGNSYTYKFNVTGQRGTLWWHAHIFWLRATVYGAIVIMPKLGTPFPFPQPAREFEIVLGEWWNNDVEEIEKQGNKMGLPPNMSDAHTINGKPGPLFPCSEKHTFAMEVEQGKTYLLRIINAALNDELFFAIAGHNMTVVEVDAVYTKPFTTQAILIAPGQTTNVLVHANQVAGRYFVATKAFMDAPIQVDNKTATAILQYKDIPNSVLPILPQLPASNDTGFALSYNKKIRSLNSAKYPANVPLKVDRNLFYTIGLGKNSCPTCLNGTRFLASINNVSFVMPQVALLQAHYFNIKGVFRADFPDRPPIPFNYTGAPLTANLASLTGTRVNKIAFNSTVELVLQDTNLLTVESHPFHLHGYNFFVVGNGIGNFDPAKDPAKYNLVDPIERNTVGVPTGGWTAIRFQADNPGVWFMHCHLELHTGWGLKTAFLVENGPNQGQSVLPPPKDLPSC