Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59798.t1 | XP_003628123.1 | 92.9 | 322 | 14 | 1 | 1 | 322 | 1 | 313 | 2.20E-154 | 555.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59798.t1 | Q9LES3 | 52.5 | 314 | 122 | 7 | 14 | 322 | 6 | 297 | 2.0e-63 | 244.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59798.t1 | G7L6Z1 | 92.9 | 322 | 14 | 1 | 1 | 322 | 1 | 313 | 1.6e-154 | 555.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene59798.t1 | TF | bZIP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049666 | MS.gene59798 | 0.80758 | 4.72E-50 | -1.69E-46 |
| MS.gene052358 | MS.gene59798 | -0.803389 | 3.57E-49 | -1.69E-46 |
| MS.gene05548 | MS.gene59798 | 0.809242 | 2.08E-50 | -1.69E-46 |
| MS.gene05954 | MS.gene59798 | 0.800855 | 1.18E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59798.t1 | MTR_8g043960 | 92.857 | 322 | 14 | 1 | 1 | 322 | 1 | 313 | 0.0 | 591 |
| MS.gene59798.t1 | MTR_3g010660 | 64.087 | 323 | 109 | 4 | 6 | 322 | 1 | 322 | 7.36e-143 | 406 |
| MS.gene59798.t1 | MTR_7g088090 | 47.006 | 334 | 146 | 8 | 6 | 322 | 1 | 320 | 1.89e-86 | 263 |
| MS.gene59798.t1 | MTR_6g026800 | 49.398 | 332 | 142 | 7 | 6 | 322 | 1 | 321 | 3.88e-83 | 254 |
| MS.gene59798.t1 | MTR_4g085910 | 36.337 | 344 | 144 | 13 | 23 | 301 | 17 | 350 | 3.32e-42 | 150 |
| MS.gene59798.t1 | MTR_5g088230 | 54.967 | 151 | 61 | 3 | 1 | 148 | 1 | 147 | 7.91e-35 | 128 |
| MS.gene59798.t1 | MTR_5g088230 | 61.364 | 88 | 33 | 1 | 235 | 322 | 183 | 269 | 3.99e-24 | 99.8 |
| MS.gene59798.t1 | MTR_7g104480 | 34.682 | 346 | 143 | 11 | 30 | 294 | 45 | 388 | 6.13e-34 | 129 |
| MS.gene59798.t1 | MTR_5g088230 | 54.110 | 146 | 60 | 3 | 6 | 148 | 1 | 142 | 7.31e-32 | 120 |
| MS.gene59798.t1 | MTR_5g088230 | 61.364 | 88 | 33 | 1 | 235 | 322 | 178 | 264 | 4.06e-24 | 99.4 |
| MS.gene59798.t1 | MTR_2g086390 | 35.088 | 285 | 126 | 12 | 27 | 294 | 11 | 253 | 3.52e-30 | 116 |
| MS.gene59798.t1 | MTR_2g086390 | 35.587 | 281 | 130 | 12 | 27 | 294 | 11 | 253 | 4.76e-30 | 115 |
| MS.gene59798.t1 | MTR_2g086340 | 37.589 | 282 | 142 | 10 | 26 | 294 | 24 | 284 | 1.32e-29 | 115 |
| MS.gene59798.t1 | MTR_3g101780 | 48.529 | 136 | 62 | 3 | 12 | 144 | 14 | 144 | 1.41e-29 | 117 |
| MS.gene59798.t1 | MTR_3g101780 | 63.768 | 69 | 22 | 1 | 229 | 297 | 333 | 398 | 4.79e-17 | 81.6 |
| MS.gene59798.t1 | MTR_5g060990 | 31.353 | 303 | 155 | 11 | 16 | 273 | 4 | 298 | 6.94e-24 | 99.8 |
| MS.gene59798.t1 | MTR_4g094390 | 53.521 | 71 | 29 | 1 | 238 | 304 | 117 | 187 | 5.29e-12 | 64.3 |
| MS.gene59798.t1 | MTR_8g075130 | 44.828 | 87 | 36 | 3 | 243 | 322 | 97 | 178 | 8.42e-12 | 63.5 |
| MS.gene59798.t1 | MTR_7g015990 | 70.000 | 50 | 12 | 1 | 251 | 297 | 120 | 169 | 1.78e-11 | 62.8 |
| MS.gene59798.t1 | MTR_5g022780 | 61.017 | 59 | 11 | 1 | 251 | 297 | 166 | 224 | 3.23e-11 | 62.8 |
| MS.gene59798.t1 | MTR_1g098590 | 62.745 | 51 | 19 | 0 | 246 | 296 | 157 | 207 | 6.50e-11 | 62.0 |
| MS.gene59798.t1 | MTR_1g098590 | 62.745 | 51 | 19 | 0 | 246 | 296 | 157 | 207 | 8.19e-11 | 61.2 |
| MS.gene59798.t1 | MTR_1g098590 | 62.745 | 51 | 19 | 0 | 246 | 296 | 157 | 207 | 9.48e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59798.t1 | AT3G56850 | 53.055 | 311 | 107 | 8 | 23 | 322 | 15 | 297 | 5.27e-90 | 271 |
| MS.gene59798.t1 | AT2G41070 | 49.007 | 302 | 94 | 9 | 23 | 320 | 15 | 260 | 1.02e-74 | 231 |
| MS.gene59798.t1 | AT2G41070 | 49.007 | 302 | 94 | 9 | 23 | 320 | 15 | 260 | 1.02e-74 | 231 |
| MS.gene59798.t1 | AT2G41070 | 49.007 | 302 | 94 | 9 | 23 | 320 | 15 | 260 | 1.02e-74 | 231 |
| MS.gene59798.t1 | AT2G41070 | 49.007 | 302 | 94 | 9 | 23 | 320 | 24 | 269 | 1.36e-74 | 231 |
| MS.gene59798.t1 | AT2G41070 | 49.281 | 278 | 81 | 9 | 23 | 296 | 15 | 236 | 4.97e-66 | 208 |
| MS.gene59798.t1 | AT2G36270 | 33.092 | 414 | 156 | 15 | 23 | 322 | 36 | 442 | 5.23e-38 | 140 |
| MS.gene59798.t1 | AT2G36270 | 33.092 | 414 | 156 | 15 | 23 | 322 | 36 | 442 | 5.23e-38 | 140 |
| MS.gene59798.t1 | AT2G36270 | 33.508 | 382 | 140 | 12 | 23 | 297 | 36 | 410 | 8.36e-37 | 137 |
| MS.gene59798.t1 | AT1G49720 | 43.407 | 182 | 83 | 5 | 1 | 167 | 1 | 177 | 1.34e-31 | 122 |
| MS.gene59798.t1 | AT1G49720 | 48.837 | 86 | 35 | 1 | 246 | 322 | 307 | 392 | 5.86e-14 | 72.4 |
| MS.gene59798.t1 | AT1G49720 | 43.407 | 182 | 83 | 5 | 1 | 167 | 1 | 177 | 1.34e-31 | 122 |
| MS.gene59798.t1 | AT1G49720 | 48.837 | 86 | 35 | 1 | 246 | 322 | 307 | 392 | 5.86e-14 | 72.4 |
| MS.gene59798.t1 | AT1G49720 | 43.407 | 182 | 83 | 5 | 1 | 167 | 1 | 177 | 1.67e-31 | 122 |
| MS.gene59798.t1 | AT1G49720 | 68.627 | 51 | 16 | 0 | 246 | 296 | 307 | 357 | 5.02e-13 | 69.7 |
| MS.gene59798.t1 | AT3G44460 | 39.373 | 287 | 124 | 13 | 26 | 294 | 37 | 291 | 1.09e-28 | 113 |
| MS.gene59798.t1 | AT3G19290 | 46.429 | 140 | 56 | 3 | 24 | 144 | 34 | 173 | 1.61e-25 | 105 |
| MS.gene59798.t1 | AT3G19290 | 46.429 | 140 | 56 | 3 | 24 | 144 | 34 | 173 | 2.08e-25 | 105 |
| MS.gene59798.t1 | AT3G19290 | 64.706 | 51 | 18 | 0 | 246 | 296 | 347 | 397 | 1.64e-13 | 71.2 |
| MS.gene59798.t1 | AT3G19290 | 46.429 | 140 | 56 | 3 | 24 | 144 | 34 | 173 | 2.82e-25 | 105 |
| MS.gene59798.t1 | AT3G19290 | 64.706 | 51 | 18 | 0 | 246 | 296 | 347 | 397 | 2.38e-13 | 70.9 |
| MS.gene59798.t1 | AT3G19290 | 46.429 | 140 | 56 | 3 | 24 | 144 | 34 | 173 | 2.88e-25 | 105 |
| MS.gene59798.t1 | AT3G19290 | 64.706 | 51 | 18 | 0 | 246 | 296 | 347 | 397 | 2.46e-13 | 70.9 |
| MS.gene59798.t1 | AT3G19290 | 46.429 | 140 | 56 | 3 | 24 | 144 | 34 | 173 | 2.88e-25 | 105 |
| MS.gene59798.t1 | AT3G19290 | 64.706 | 51 | 18 | 0 | 246 | 296 | 347 | 397 | 2.46e-13 | 70.9 |
| MS.gene59798.t1 | AT4G34000 | 39.130 | 161 | 58 | 3 | 24 | 144 | 27 | 187 | 8.18e-24 | 101 |
| MS.gene59798.t1 | AT4G34000 | 41.447 | 152 | 74 | 6 | 177 | 317 | 293 | 440 | 5.62e-16 | 78.6 |
| MS.gene59798.t1 | AT4G34000 | 39.130 | 161 | 58 | 3 | 24 | 144 | 27 | 187 | 1.04e-23 | 101 |
| MS.gene59798.t1 | AT4G34000 | 44.444 | 135 | 60 | 6 | 177 | 301 | 293 | 422 | 8.27e-16 | 78.2 |
| MS.gene59798.t1 | AT4G34000 | 39.130 | 161 | 58 | 3 | 24 | 144 | 27 | 187 | 1.04e-23 | 101 |
| MS.gene59798.t1 | AT4G34000 | 44.444 | 135 | 60 | 6 | 177 | 301 | 293 | 422 | 8.27e-16 | 78.2 |
| MS.gene59798.t1 | AT4G34000 | 39.130 | 161 | 58 | 3 | 24 | 144 | 27 | 187 | 1.04e-23 | 101 |
| MS.gene59798.t1 | AT4G34000 | 44.444 | 135 | 60 | 6 | 177 | 301 | 293 | 422 | 8.27e-16 | 78.2 |
| MS.gene59798.t1 | AT5G42910 | 28.763 | 372 | 167 | 16 | 23 | 319 | 19 | 367 | 4.97e-23 | 98.6 |
| MS.gene59798.t1 | AT1G45249 | 41.176 | 136 | 62 | 3 | 24 | 144 | 21 | 153 | 3.04e-21 | 93.6 |
| MS.gene59798.t1 | AT1G45249 | 41.176 | 136 | 62 | 3 | 24 | 144 | 21 | 153 | 3.04e-21 | 93.6 |
| MS.gene59798.t1 | AT1G45249 | 34.392 | 189 | 91 | 5 | 24 | 190 | 36 | 213 | 5.24e-21 | 93.2 |
| MS.gene59798.t1 | AT1G45249 | 52.632 | 95 | 33 | 2 | 236 | 322 | 341 | 431 | 3.11e-17 | 82.4 |
| MS.gene59798.t1 | AT1G45249 | 34.392 | 189 | 91 | 5 | 24 | 190 | 36 | 213 | 5.24e-21 | 93.2 |
| MS.gene59798.t1 | AT1G45249 | 52.632 | 95 | 33 | 2 | 236 | 322 | 341 | 431 | 3.11e-17 | 82.4 |
| MS.gene59798.t1 | AT1G45249 | 34.392 | 189 | 91 | 5 | 24 | 190 | 21 | 198 | 6.27e-21 | 92.8 |
| MS.gene59798.t1 | AT1G45249 | 52.632 | 95 | 33 | 2 | 236 | 322 | 326 | 416 | 2.17e-17 | 82.8 |
| MS.gene59798.t1 | AT1G45249 | 41.176 | 136 | 62 | 3 | 24 | 144 | 21 | 153 | 6.57e-21 | 92.8 |
| MS.gene59798.t1 | AT1G45249 | 41.176 | 136 | 62 | 3 | 24 | 144 | 21 | 153 | 6.57e-21 | 92.8 |
| MS.gene59798.t1 | AT1G45249 | 41.176 | 136 | 62 | 3 | 24 | 144 | 21 | 153 | 6.57e-21 | 92.8 |
| MS.gene59798.t1 | AT1G45249 | 41.176 | 136 | 62 | 3 | 24 | 144 | 21 | 153 | 6.57e-21 | 92.8 |
| MS.gene59798.t1 | AT1G45249 | 34.392 | 189 | 91 | 5 | 24 | 190 | 21 | 198 | 9.18e-21 | 92.4 |
| MS.gene59798.t1 | AT1G45249 | 47.170 | 106 | 33 | 3 | 236 | 322 | 326 | 427 | 1.63e-14 | 74.3 |
| MS.gene59798.t1 | AT5G44080 | 33.047 | 233 | 105 | 10 | 100 | 320 | 120 | 313 | 1.93e-18 | 84.7 |
| MS.gene59798.t1 | AT4G35900 | 51.136 | 88 | 38 | 3 | 236 | 322 | 202 | 285 | 1.27e-16 | 79.0 |
| MS.gene59798.t1 | AT2G17770 | 39.850 | 133 | 72 | 4 | 193 | 322 | 68 | 195 | 1.08e-13 | 69.3 |
| MS.gene59798.t1 | AT1G03970 | 30.568 | 229 | 85 | 8 | 100 | 310 | 85 | 257 | 3.02e-12 | 66.2 |
Find 82 sgRNAs with CRISPR-Local
Find 128 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCATTGGGTGCACCTCTTA+TGG | 0.223647 | 8.3:-54924946 | MS.gene59798:CDS |
| ATTCGACGTAGCTGATATTT+AGG | 0.229724 | 8.3:+54923744 | None:intergenic |
| GATGACATTGGAGGATTTCT+TGG | 0.249290 | 8.3:-54925216 | MS.gene59798:CDS |
| CAAAATCAATTAGGTGACTT+AGG | 0.335660 | 8.3:-54925488 | MS.gene59798:CDS |
| CGAGAGAGACAAACTACTTT+AGG | 0.337274 | 8.3:-54925242 | MS.gene59798:CDS |
| CGCAGATGCCTGGAAGAAAA+AGG | 0.341085 | 8.3:-54924908 | MS.gene59798:CDS |
| GATGAAGTTCAAAATCAATT+AGG | 0.351054 | 8.3:-54925497 | MS.gene59798:CDS |
| AAGGATCACTCCAGAGGATA+TGG | 0.352037 | 8.3:-54924889 | MS.gene59798:CDS |
| CTGTTTCCGTGCTCTTGAAC+GGG | 0.379424 | 8.3:+54924809 | None:intergenic |
| GCCTTCATTGTCCGTATTCG+TGG | 0.383060 | 8.3:+54925397 | None:intergenic |
| CCGTTCAAGAGCACGGAAAC+AGG | 0.394263 | 8.3:-54924808 | MS.gene59798:intron |
| TCTACAACGTCAAGCTAGTC+TGG | 0.406297 | 8.3:-54925351 | MS.gene59798:CDS |
| GCATTGGGTGCACCTCTTAT+GGG | 0.408513 | 8.3:-54924945 | MS.gene59798:CDS |
| GCAGGCTTACACAAATGAAT+TGG | 0.412384 | 8.3:-54924683 | MS.gene59798:intron |
| CATTGTCCGTATTCGTGGAC+TGG | 0.421785 | 8.3:+54925402 | None:intergenic |
| AGAGGACTGTGGATGAGGTT+TGG | 0.422682 | 8.3:-54925307 | MS.gene59798:CDS |
| TCTAGGTTCATACTGCTTAG+TGG | 0.432596 | 8.3:+54925461 | None:intergenic |
| TGATATTTAGGTTCAGCAAT+TGG | 0.433568 | 8.3:+54923756 | None:intergenic |
| AAAATCAATTAGGTGACTTA+GGG | 0.436185 | 8.3:-54925487 | MS.gene59798:CDS |
| AGTATCACAGTTTCCTCTGC+AGG | 0.436415 | 8.3:-54925114 | MS.gene59798:CDS |
| TCCGACACAGTTCCCATAAG+AGG | 0.453901 | 8.3:+54924933 | None:intergenic |
| TCCGTTTGCAGATAGTCATA+TGG | 0.455759 | 8.3:-54924967 | MS.gene59798:CDS |
| AGAAAAGGATGATCAAGAAT+CGG | 0.457820 | 8.3:-54924842 | MS.gene59798:CDS |
| GCAGATAGTCATATGGCATT+GGG | 0.461753 | 8.3:-54924960 | MS.gene59798:CDS |
| CTGTTGAGAGAAGGCAGAAA+AGG | 0.461801 | 8.3:-54924857 | MS.gene59798:CDS |
| TGCAGATAGTCATATGGCAT+TGG | 0.465956 | 8.3:-54924961 | MS.gene59798:CDS |
| CCTGTTTCCGTGCTCTTGAA+CGG | 0.468292 | 8.3:+54924808 | None:intergenic |
| GTGTCGGATACGCAGATGCC+TGG | 0.469471 | 8.3:-54924918 | MS.gene59798:CDS |
| GGTTCAGCAATTGGTGTACA+TGG | 0.473186 | 8.3:+54923765 | None:intergenic |
| CAGCAAGAGGACTGTGGATG+AGG | 0.475392 | 8.3:-54925312 | MS.gene59798:CDS |
| TAAATGGGAACTCAGACAAT+GGG | 0.478433 | 8.3:-54925605 | MS.gene59798:CDS |
| GAGGATTTCTTGGTGAAAGC+AGG | 0.483426 | 8.3:-54925206 | MS.gene59798:CDS |
| GGGTCTCAAGGTGGTGGAGA+TGG | 0.487387 | 8.3:-54925584 | MS.gene59798:CDS |
| GTCTTCTCCACCATATCCTC+TGG | 0.490528 | 8.3:+54924879 | None:intergenic |
| ATAAATGGGAACTCAGACAA+TGG | 0.492628 | 8.3:-54925606 | None:intergenic |
| TGATACTGCCACATTTGAAT+CGG | 0.495093 | 8.3:+54925130 | None:intergenic |
| TTTAGGAGAGATGACATTGG+AGG | 0.495208 | 8.3:-54925225 | MS.gene59798:CDS |
| GGGGATTTATATGCCTAGCC+AGG | 0.498570 | 8.3:-54925036 | MS.gene59798:CDS |
| TAAAGTTTCACGCCTGGAAG+AGG | 0.502778 | 8.3:-54924656 | MS.gene59798:CDS |
| CAGCCGTTACACATGGGGTC+TGG | 0.513878 | 8.3:-54925005 | MS.gene59798:CDS |
| GCCATATGACTATCTGCAAA+CGG | 0.520435 | 8.3:+54924966 | None:intergenic |
| GAAAAGGATGATCAAGAATC+GGG | 0.521274 | 8.3:-54924841 | MS.gene59798:CDS |
| CATGGGGTCTGGTGTTTCGA+TGG | 0.522204 | 8.3:-54924994 | MS.gene59798:CDS |
| ACTCAGACAATGGGGTCTCA+AGG | 0.525496 | 8.3:-54925596 | MS.gene59798:CDS |
| TGTTGTAGATGCTGATATTG+TGG | 0.526222 | 8.3:+54925068 | None:intergenic |
| AGTGAACCAGTCCACGAATA+CGG | 0.529125 | 8.3:-54925408 | MS.gene59798:CDS |
| CAAGGTGGTGGAGATGGTAG+TGG | 0.530920 | 8.3:-54925578 | MS.gene59798:CDS |
| GCATCTACAACAAAGTTCCA+TGG | 0.531471 | 8.3:-54925057 | MS.gene59798:CDS |
| AGTTTCCTCTGCAGGGTCAG+TGG | 0.540111 | 8.3:-54925106 | MS.gene59798:CDS |
| CATCTACAACAAAGTTCCAT+GGG | 0.551430 | 8.3:-54925056 | MS.gene59798:CDS |
| TAAAAGCTGCATTCAGCAAG+AGG | 0.554122 | 8.3:-54925325 | MS.gene59798:CDS |
| GGAGATTAAAGTTTCACGCC+TGG | 0.555439 | 8.3:-54924662 | MS.gene59798:CDS |
| AGATGCTGATATTGTGGTTG+CGG | 0.557999 | 8.3:+54925074 | None:intergenic |
| ACAATGGGGTCTCAAGGTGG+TGG | 0.565619 | 8.3:-54925590 | MS.gene59798:CDS |
| ACCTCTTATGGGAACTGTGT+CGG | 0.579301 | 8.3:-54924934 | MS.gene59798:CDS |
| TCCACGAATACGGACAATGA+AGG | 0.580287 | 8.3:-54925398 | MS.gene59798:CDS |
| CTGCTGCCCGTTCAAGAGCA+CGG | 0.591970 | 8.3:-54924815 | MS.gene59798:CDS |
| TATAGCACAGCCGTTACACA+TGG | 0.595784 | 8.3:-54925012 | MS.gene59798:CDS |
| TGGAGAAGACTGTTGAGAGA+AGG | 0.597925 | 8.3:-54924866 | MS.gene59798:CDS |
| GTGGAGATGGTAGTGGAAGA+CGG | 0.609087 | 8.3:-54925571 | MS.gene59798:CDS |
| ACACCAGACCCCATGTGTAA+CGG | 0.609930 | 8.3:+54925002 | None:intergenic |
| CACAACTCCACTCTCTTGTG+AGG | 0.615377 | 8.3:-54925547 | MS.gene59798:CDS |
| GTATCACAGTTTCCTCTGCA+GGG | 0.615727 | 8.3:-54925113 | MS.gene59798:CDS |
| TGAAATGCTGAGGAAACGAA+AGG | 0.623305 | 8.3:-54924629 | MS.gene59798:intron |
| GGCTGTGCTATACCCTGGCT+AGG | 0.624273 | 8.3:+54925023 | None:intergenic |
| GAATACTGATACTACGGCTG+CGG | 0.624343 | 8.3:-54925153 | MS.gene59798:CDS |
| GATCACTCCAGAGGATATGG+TGG | 0.634046 | 8.3:-54924886 | MS.gene59798:CDS |
| TAGGTACACAGATCTCAGAA+TGG | 0.634421 | 8.3:+54923711 | None:intergenic |
| AAGAGGAAAATGAAATGCTG+AGG | 0.635284 | 8.3:-54924639 | MS.gene59798:CDS |
| TACTTTAGGAGAGATGACAT+TGG | 0.639124 | 8.3:-54925228 | MS.gene59798:CDS |
| GGCTGCGGCCGATTCAAATG+TGG | 0.639880 | 8.3:-54925138 | MS.gene59798:CDS |
| GGGATTTATATGCCTAGCCA+GGG | 0.641870 | 8.3:-54925035 | MS.gene59798:CDS |
| TGTAACCACTGACCCTGCAG+AGG | 0.647637 | 8.3:+54925101 | None:intergenic |
| GCTAGGCATATAAATCCCCA+TGG | 0.652039 | 8.3:+54925040 | None:intergenic |
| TAGCACAGCCGTTACACATG+GGG | 0.656286 | 8.3:-54925010 | MS.gene59798:CDS |
| CAGACAATGGGGTCTCAAGG+TGG | 0.688721 | 8.3:-54925593 | MS.gene59798:CDS |
| GTAACGGCTGTGCTATACCC+TGG | 0.689448 | 8.3:+54925018 | None:intergenic |
| ATAGCACAGCCGTTACACAT+GGG | 0.689708 | 8.3:-54925011 | MS.gene59798:CDS |
| TGCATTCAGCAAGAGGACTG+TGG | 0.691129 | 8.3:-54925318 | MS.gene59798:CDS |
| AAATGGGAACTCAGACAATG+GGG | 0.712282 | 8.3:-54925604 | MS.gene59798:CDS |
| ATCTACAACAAAGTTCCATG+GGG | 0.739377 | 8.3:-54925055 | MS.gene59798:CDS |
| CCAACAAAAGAAAGACAACG+AGG | 0.748926 | 8.3:-54925276 | MS.gene59798:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAAAACAAATCAAATAATA+AGG | + | chr8.3:54924584-54924603 | None:intergenic | 15.0% |
| !! | AATAAATCAAATTCCAAATC+AGG | + | chr8.3:54925295-54925314 | None:intergenic | 20.0% |
| !! | TAAAAAGAATACTGATACTA+CGG | - | chr8.3:54924168-54924187 | MS.gene59798:intron | 20.0% |
| !! | TAGTTACTGGATTATTTATT+AGG | - | chr8.3:54925051-54925070 | MS.gene59798:CDS | 20.0% |
| !!! | AAAAGTTATTATTCAGGTTT+AGG | + | chr8.3:54924620-54924639 | None:intergenic | 20.0% |
| !!! | AAAGTTATTATTCAGGTTTA+GGG | + | chr8.3:54924619-54924638 | None:intergenic | 20.0% |
| !!! | ATTTTTACCTGTTAAAATCA+CGG | - | chr8.3:54925428-54925447 | MS.gene59798:CDS | 20.0% |
| !!! | TGATTTGGAATTTGATTTAT+TGG | - | chr8.3:54925294-54925313 | MS.gene59798:CDS | 20.0% |
| ! | AAAATCAATTAGGTGACTTA+GGG | - | chr8.3:54923840-54923859 | MS.gene59798:intron | 25.0% |
| ! | ACTGCTAATTGAATGAAAAA+GGG | + | chr8.3:54925334-54925353 | None:intergenic | 25.0% |
| ! | GATGAAGTTCAAAATCAATT+AGG | - | chr8.3:54923830-54923849 | MS.gene59798:intron | 25.0% |
| ! | GCTTGTAAAAGTTATTATTC+AGG | + | chr8.3:54924626-54924645 | None:intergenic | 25.0% |
| ! | GTGTTAGATTTGAATGAAAT+AGG | - | chr8.3:54924970-54924989 | MS.gene59798:CDS | 25.0% |
| ! | TAATCTGAATAGTCAATTAG+AGG | + | chr8.3:54925495-54925514 | None:intergenic | 25.0% |
| ! | TACTGCTAATTGAATGAAAA+AGG | + | chr8.3:54925335-54925354 | None:intergenic | 25.0% |
| ! | TTATAATTTCAGCACAAGAT+AGG | - | chr8.3:54924825-54924844 | MS.gene59798:CDS | 25.0% |
| !! | GAATAATAACTTTTACAAGC+AGG | - | chr8.3:54924626-54924645 | MS.gene59798:intron | 25.0% |
| !! | GTAGTCATTAAGAATTTTTC+AGG | - | chr8.3:54925110-54925129 | MS.gene59798:CDS | 25.0% |
| !! | TTTTTACCTGTTAAAATCAC+GGG | - | chr8.3:54925429-54925448 | MS.gene59798:CDS | 25.0% |
| AATGTACAAACTACTCAATC+TGG | + | chr8.3:54924799-54924818 | None:intergenic | 30.0% | |
| AGAAAAGGATGATCAAGAAT+CGG | - | chr8.3:54924485-54924504 | MS.gene59798:intron | 30.0% | |
| ATACTTGATATTGCCTGATT+TGG | - | chr8.3:54925279-54925298 | MS.gene59798:CDS | 30.0% | |
| ATGTACAAACTACTCAATCT+GGG | + | chr8.3:54924798-54924817 | None:intergenic | 30.0% | |
| CAAAATCAATTAGGTGACTT+AGG | - | chr8.3:54923839-54923858 | MS.gene59798:intron | 30.0% | |
| CATTCAATTAGCAGTATTTG+TGG | - | chr8.3:54925339-54925358 | MS.gene59798:CDS | 30.0% | |
| TTAACAGGTAAAAATGACCA+TGG | + | chr8.3:54925423-54925442 | None:intergenic | 30.0% | |
| ! | ACTAGGTAAAGATTAGTTAC+TGG | - | chr8.3:54925038-54925057 | MS.gene59798:CDS | 30.0% |
| ! | TGATATTTAGGTTCAGCAAT+TGG | + | chr8.3:54925574-54925593 | None:intergenic | 30.0% |
| ! | TTGAGTAGTTTGTACATTTG+TGG | - | chr8.3:54924801-54924820 | MS.gene59798:intron | 30.0% |
| AAGAGGAAAATGAAATGCTG+AGG | - | chr8.3:54924688-54924707 | MS.gene59798:intron | 35.0% | |
| AATATTTGTCCTCAACATGC+AGG | - | chr8.3:54925522-54925541 | MS.gene59798:CDS | 35.0% | |
| ATCTACAACAAAGTTCCATG+GGG | - | chr8.3:54924272-54924291 | MS.gene59798:intron | 35.0% | |
| ATTCGACGTAGCTGATATTT+AGG | + | chr8.3:54925586-54925605 | None:intergenic | 35.0% | |
| CATCTACAACAAAGTTCCAT+GGG | - | chr8.3:54924271-54924290 | MS.gene59798:intron | 35.0% | |
| CTACGTAAAAGATAGAGCAT+AGG | + | chr8.3:54925094-54925113 | None:intergenic | 35.0% | |
| CTATATGCACATTGTATCCA+TGG | - | chr8.3:54925403-54925422 | MS.gene59798:CDS | 35.0% | |
| CTGCTCTCAATATACTACAA+TGG | - | chr8.3:54925217-54925236 | MS.gene59798:CDS | 35.0% | |
| GAAAAGGATGATCAAGAATC+GGG | - | chr8.3:54924486-54924505 | MS.gene59798:intron | 35.0% | |
| GAATAGTCAATTAGAGGTGA+AGG | + | chr8.3:54925489-54925508 | None:intergenic | 35.0% | |
| GGTTGATCAATGGATCTATT+TGG | + | chr8.3:54925003-54925022 | None:intergenic | 35.0% | |
| TGATACTGCCACATTTGAAT+CGG | + | chr8.3:54924200-54924219 | None:intergenic | 35.0% | |
| TGTACAAACTACTCAATCTG+GGG | + | chr8.3:54924797-54924816 | None:intergenic | 35.0% | |
| TGTTGTAGATGCTGATATTG+TGG | + | chr8.3:54924262-54924281 | None:intergenic | 35.0% | |
| ! | AAATATCTGCTGCTTCATGA+TGG | - | chr8.3:54925252-54925271 | MS.gene59798:CDS | 35.0% |
| ! | ACGAGCTTCTGAAAAATGTA+TGG | - | chr8.3:54923888-54923907 | MS.gene59798:intron | 35.0% |
| ! | TACTTTAGGAGAGATGACAT+TGG | - | chr8.3:54924099-54924118 | MS.gene59798:intron | 35.0% |
| !! | AGAGATATCAGGTTGATCAA+TGG | + | chr8.3:54925013-54925032 | None:intergenic | 35.0% |
| !! | TTTGTTCCCGTGATTTTAAC+AGG | + | chr8.3:54925438-54925457 | None:intergenic | 35.0% |
| !!! | GTTAGTTCTGACACAACTTT+TGG | - | chr8.3:54925188-54925207 | MS.gene59798:CDS | 35.0% |
| AAGAAAAAGGATCACTCCAG+AGG | - | chr8.3:54924432-54924451 | MS.gene59798:intron | 40.0% | |
| AGATGCTGATATTGTGGTTG+CGG | + | chr8.3:54924256-54924275 | None:intergenic | 40.0% | |
| CCAACAAAAGAAAGACAACG+AGG | - | chr8.3:54924051-54924070 | MS.gene59798:intron | 40.0% | |
| CCTAGTGTATCAGAGATATC+AGG | + | chr8.3:54925024-54925043 | None:intergenic | 40.0% | |
| CCTGATATCTCTGATACACT+AGG | - | chr8.3:54925021-54925040 | MS.gene59798:CDS | 40.0% | |
| CGAGAGAGACAAACTACTTT+AGG | - | chr8.3:54924085-54924104 | MS.gene59798:intron | 40.0% | |
| GATGACATTGGAGGATTTCT+TGG | - | chr8.3:54924111-54924130 | MS.gene59798:intron | 40.0% | |
| GCAGATAGTCATATGGCATT+GGG | - | chr8.3:54924367-54924386 | MS.gene59798:intron | 40.0% | |
| GCAGGCTTACACAAATGAAT+TGG | - | chr8.3:54924644-54924663 | MS.gene59798:CDS | 40.0% | |
| GCATCTACAACAAAGTTCCA+TGG | - | chr8.3:54924270-54924289 | MS.gene59798:intron | 40.0% | |
| GCCATATGACTATCTGCAAA+CGG | + | chr8.3:54924364-54924383 | None:intergenic | 40.0% | |
| TAAAAGCTGCATTCAGCAAG+AGG | - | chr8.3:54924002-54924021 | MS.gene59798:intron | 40.0% | |
| TACTCAATCTGGGGAATGAA+AGG | + | chr8.3:54924788-54924807 | None:intergenic | 40.0% | |
| TCCGTTTGCAGATAGTCATA+TGG | - | chr8.3:54924360-54924379 | MS.gene59798:intron | 40.0% | |
| TCTAGGTTCATACTGCTTAG+TGG | + | chr8.3:54923869-54923888 | None:intergenic | 40.0% | |
| TCTGGGGAATGAAAGGAATT+AGG | + | chr8.3:54924781-54924800 | None:intergenic | 40.0% | |
| TGAAATGCTGAGGAAACGAA+AGG | - | chr8.3:54924698-54924717 | MS.gene59798:intron | 40.0% | |
| TGCAGATAGTCATATGGCAT+TGG | - | chr8.3:54924366-54924385 | MS.gene59798:intron | 40.0% | |
| TTTAGGAGAGATGACATTGG+AGG | - | chr8.3:54924102-54924121 | MS.gene59798:intron | 40.0% | |
| ! | AATTTTGCCTCACAAGAGAG+TGG | + | chr8.3:54923790-54923809 | None:intergenic | 40.0% |
| ! | CAGTCTCAGTTTCTGGATTA+AGG | - | chr8.3:54924857-54924876 | MS.gene59798:CDS | 40.0% |
| ! | CCTCGTTGTCTTTCTTTTGT+TGG | + | chr8.3:54924054-54924073 | None:intergenic | 40.0% |
| ! | GCATTTCATTTTCCTCTTCC+AGG | + | chr8.3:54924686-54924705 | None:intergenic | 40.0% |
| !! | CATTTTTCAGAAGCTCGTCT+AGG | + | chr8.3:54923886-54923905 | None:intergenic | 40.0% |
| AAGGATCACTCCAGAGGATA+TGG | - | chr8.3:54924438-54924457 | MS.gene59798:intron | 45.0% | |
| AATGCAAATGACGCATGCTG+AGG | + | chr8.3:54924914-54924933 | None:intergenic | 45.0% | |
| ACAGTCTCAGTCTCAGTTTC+TGG | - | chr8.3:54924850-54924869 | MS.gene59798:CDS | 45.0% | |
| ACCTCTTATGGGAACTGTGT+CGG | - | chr8.3:54924393-54924412 | MS.gene59798:intron | 45.0% | |
| AGTATCACAGTTTCCTCTGC+AGG | - | chr8.3:54924213-54924232 | MS.gene59798:intron | 45.0% | |
| AGTGAACCAGTCCACGAATA+CGG | - | chr8.3:54923919-54923938 | MS.gene59798:intron | 45.0% | |
| ATAGCACAGCCGTTACACAT+GGG | - | chr8.3:54924316-54924335 | MS.gene59798:intron | 45.0% | |
| CTGTTGAGAGAAGGCAGAAA+AGG | - | chr8.3:54924470-54924489 | MS.gene59798:intron | 45.0% | |
| GAATACTGATACTACGGCTG+CGG | - | chr8.3:54924174-54924193 | MS.gene59798:intron | 45.0% | |
| GAGGATTTCTTGGTGAAAGC+AGG | - | chr8.3:54924121-54924140 | MS.gene59798:intron | 45.0% | |
| GCTAGGCATATAAATCCCCA+TGG | + | chr8.3:54924290-54924309 | None:intergenic | 45.0% | |
| GGAGATTAAAGTTTCACGCC+TGG | - | chr8.3:54924665-54924684 | MS.gene59798:CDS | 45.0% | |
| GGGATTTATATGCCTAGCCA+GGG | - | chr8.3:54924292-54924311 | MS.gene59798:intron | 45.0% | |
| GGTTCAGCAATTGGTGTACA+TGG | + | chr8.3:54925565-54925584 | None:intergenic | 45.0% | |
| GTATCACAGTTTCCTCTGCA+GGG | - | chr8.3:54924214-54924233 | MS.gene59798:intron | 45.0% | |
| TAAAGTTTCACGCCTGGAAG+AGG | - | chr8.3:54924671-54924690 | MS.gene59798:CDS | 45.0% | |
| TATAGCACAGCCGTTACACA+TGG | - | chr8.3:54924315-54924334 | MS.gene59798:intron | 45.0% | |
| TCCACGAATACGGACAATGA+AGG | - | chr8.3:54923929-54923948 | MS.gene59798:intron | 45.0% | |
| TCTACAACGTCAAGCTAGTC+TGG | - | chr8.3:54923976-54923995 | MS.gene59798:intron | 45.0% | |
| TGGAGAAGACTGTTGAGAGA+AGG | - | chr8.3:54924461-54924480 | MS.gene59798:intron | 45.0% | |
| ! | GGAGTGATCCTTTTTCTTCC+AGG | + | chr8.3:54924430-54924449 | None:intergenic | 45.0% |
| ACACCAGACCCCATGTGTAA+CGG | + | chr8.3:54924328-54924347 | None:intergenic | 50.0% | |
| ACTCAGACAATGGGGTCTCA+AGG | - | chr8.3:54923731-54923750 | MS.gene59798:CDS | 50.0% | |
| AGAGGACTGTGGATGAGGTT+TGG | - | chr8.3:54924020-54924039 | MS.gene59798:intron | 50.0% | |
| CACAACTCCACTCTCTTGTG+AGG | - | chr8.3:54923780-54923799 | MS.gene59798:CDS | 50.0% | |
| CATTGTCCGTATTCGTGGAC+TGG | + | chr8.3:54923928-54923947 | None:intergenic | 50.0% | |
| CCTGTTTCCGTGCTCTTGAA+CGG | + | chr8.3:54924522-54924541 | None:intergenic | 50.0% | |
| CGCAGATGCCTGGAAGAAAA+AGG | - | chr8.3:54924419-54924438 | MS.gene59798:intron | 50.0% | |
| CTGTTTCCGTGCTCTTGAAC+GGG | + | chr8.3:54924521-54924540 | None:intergenic | 50.0% | |
| GATCACTCCAGAGGATATGG+TGG | - | chr8.3:54924441-54924460 | MS.gene59798:intron | 50.0% | |
| GCATTGGGTGCACCTCTTAT+GGG | - | chr8.3:54924382-54924401 | MS.gene59798:intron | 50.0% | |
| GCCTTCATTGTCCGTATTCG+TGG | + | chr8.3:54923933-54923952 | None:intergenic | 50.0% | |
| GGGGATTTATATGCCTAGCC+AGG | - | chr8.3:54924291-54924310 | MS.gene59798:intron | 50.0% | |
| GTCTTCTCCACCATATCCTC+TGG | + | chr8.3:54924451-54924470 | None:intergenic | 50.0% | |
| TAGCACAGCCGTTACACATG+GGG | - | chr8.3:54924317-54924336 | MS.gene59798:intron | 50.0% | |
| TCCGACACAGTTCCCATAAG+AGG | + | chr8.3:54924397-54924416 | None:intergenic | 50.0% | |
| TGCATTCAGCAAGAGGACTG+TGG | - | chr8.3:54924009-54924028 | MS.gene59798:intron | 50.0% | |
| ! | TCTCTAGCTCCTGCATGTTG+AGG | + | chr8.3:54925534-54925553 | None:intergenic | 50.0% |
| !! | GTGGAGATGGTAGTGGAAGA+CGG | - | chr8.3:54923756-54923775 | MS.gene59798:CDS | 50.0% |
| ACAATGGGGTCTCAAGGTGG+TGG | - | chr8.3:54923737-54923756 | MS.gene59798:CDS | 55.0% | |
| AGTTTCCTCTGCAGGGTCAG+TGG | - | chr8.3:54924221-54924240 | MS.gene59798:intron | 55.0% | |
| CAGACAATGGGGTCTCAAGG+TGG | - | chr8.3:54923734-54923753 | MS.gene59798:CDS | 55.0% | |
| CAGCAAGAGGACTGTGGATG+AGG | - | chr8.3:54924015-54924034 | MS.gene59798:intron | 55.0% | |
| CATGGGGTCTGGTGTTTCGA+TGG | - | chr8.3:54924333-54924352 | MS.gene59798:intron | 55.0% | |
| GTAACGGCTGTGCTATACCC+TGG | + | chr8.3:54924312-54924331 | None:intergenic | 55.0% | |
| TGTAACCACTGACCCTGCAG+AGG | + | chr8.3:54924229-54924248 | None:intergenic | 55.0% | |
| ! | CCGTTCAAGAGCACGGAAAC+AGG | - | chr8.3:54924519-54924538 | MS.gene59798:intron | 55.0% |
| ! | GGCATTGGGTGCACCTCTTA+TGG | - | chr8.3:54924381-54924400 | MS.gene59798:intron | 55.0% |
| !! | CAAGGTGGTGGAGATGGTAG+TGG | - | chr8.3:54923749-54923768 | MS.gene59798:CDS | 55.0% |
| CAGCCGTTACACATGGGGTC+TGG | - | chr8.3:54924322-54924341 | MS.gene59798:intron | 60.0% | |
| CTGCTGCCCGTTCAAGAGCA+CGG | - | chr8.3:54924512-54924531 | MS.gene59798:intron | 60.0% | |
| GGCTGTGCTATACCCTGGCT+AGG | + | chr8.3:54924307-54924326 | None:intergenic | 60.0% | |
| GTGTCGGATACGCAGATGCC+TGG | - | chr8.3:54924409-54924428 | MS.gene59798:intron | 60.0% | |
| ! | GGCTGCGGCCGATTCAAATG+TGG | - | chr8.3:54924189-54924208 | MS.gene59798:intron | 60.0% |
| !! | GGGTCTCAAGGTGGTGGAGA+TGG | - | chr8.3:54923743-54923762 | MS.gene59798:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 54923725 | 54925624 | 54923725 | ID=MS.gene59798 |
| chr8.3 | mRNA | 54923725 | 54925624 | 54923725 | ID=MS.gene59798.t1;Parent=MS.gene59798 |
| chr8.3 | exon | 54924809 | 54925624 | 54924809 | ID=MS.gene59798.t1.exon1;Parent=MS.gene59798.t1 |
| chr8.3 | CDS | 54924809 | 54925624 | 54924809 | ID=cds.MS.gene59798.t1;Parent=MS.gene59798.t1 |
| chr8.3 | exon | 54924630 | 54924701 | 54924630 | ID=MS.gene59798.t1.exon2;Parent=MS.gene59798.t1 |
| chr8.3 | CDS | 54924630 | 54924701 | 54924630 | ID=cds.MS.gene59798.t1;Parent=MS.gene59798.t1 |
| chr8.3 | exon | 54923725 | 54923805 | 54923725 | ID=MS.gene59798.t1.exon3;Parent=MS.gene59798.t1 |
| chr8.3 | CDS | 54923725 | 54923805 | 54923725 | ID=cds.MS.gene59798.t1;Parent=MS.gene59798.t1 |
| Gene Sequence |
| Protein sequence |
