AlfalfaGEDB | MS.gene59958

M. sativa cultivar XinJiangDaYe / MS.gene59958

KEGG: -
GO: GO:0003674 GO:0003824 GO:0009055 GO:0016491
PFAM: PF02298.20

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59958.t1	XP_003609366.1	97.3	370	10	0	1	370	1	370	3.20E-117	431.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59958.t1	O82081	43.2	125	67	2	9	133	6	126	5.7e-22	106.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59958.t1	G7JE55	97.3	370	10	0	1	370	1	370	2.3e-117	431.8

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050363	MS.gene59958	0.846213	2.50E-59	-1.69E-46
MS.gene050381	MS.gene59958	0.809927	1.49E-50	-1.69E-46
MS.gene053319	MS.gene59958	0.806429	8.26E-50	-1.69E-46
MS.gene056313	MS.gene59958	0.817698	2.88E-52	-1.69E-46
MS.gene056408	MS.gene59958	0.83055	2.76E-55	-1.69E-46
MS.gene060808	MS.gene59958	0.959415	2.04E-117	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59958.t1	MTR_4g114870	97.297	370	10	0	1	370	1	370	0.0	704
MS.gene59958.t1	MTR_6g083240	57.225	173	60	4	1	171	1	161	2.34e-53	174
MS.gene59958.t1	MTR_6g083240	48.227	141	66	3	190	327	26	162	2.06e-35	128
MS.gene59958.t1	MTR_0334s0010	46.875	128	65	3	1	128	1	125	2.04e-31	118
MS.gene59958.t1	MTR_0334s0010	47.273	110	56	2	181	290	18	125	7.76e-28	108
MS.gene59958.t1	MTR_5g006040	43.885	139	70	4	29	166	26	157	3.15e-31	117
MS.gene59958.t1	MTR_5g006040	37.931	174	85	4	190	362	25	176	2.21e-30	115
MS.gene59958.t1	MTR_4g067200	44.531	128	66	4	3	128	5	129	1.41e-30	115
MS.gene59958.t1	MTR_4g067200	42.017	119	65	2	185	301	22	138	6.88e-26	102
MS.gene59958.t1	MTR_4g066110	44.531	128	66	4	3	128	5	129	1.41e-30	115

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59958.t1	AT2G32300	41.497	147	82	2	9	155	6	148	2.06e-30	117
MS.gene59958.t1	AT2G32300	39.716	141	78	2	191	331	26	159	7.59e-24	99.8
MS.gene59958.t1	AT2G32300	41.497	147	82	2	9	155	36	178	7.49e-30	116
MS.gene59958.t1	AT2G32300	39.716	141	78	2	191	331	56	189	2.43e-23	99.0
MS.gene59958.t1	AT2G31050	39.655	174	96	2	10	177	10	180	2.07e-29	112
MS.gene59958.t1	AT2G31050	42.017	119	66	1	185	303	23	138	5.83e-25	100
MS.gene59958.t1	AT5G20230	46.721	122	63	2	31	151	27	147	1.92e-28	110
MS.gene59958.t1	AT5G20230	37.430	179	105	3	191	369	25	196	3.28e-28	109
MS.gene59958.t1	AT3G17675	51.923	104	42	3	27	128	30	127	5.12e-28	107
MS.gene59958.t1	AT3G17675	40.000	100	56	1	191	290	32	127	2.02e-20	86.7

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Find 127 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCACCGGCACCAACCA+AGG	0.541334	8.3:-12499928	MS.gene59958:CDS
AAAATGCAGCTGCAATTGAG+AGG	0.622849	8.3:+12498745	None:intergenic
AACATTAAGGTTCTTTGCCA+TGG	0.467663	8.3:+12500480	None:intergenic
AACTCCAAGAGACGCGGCAC+CGG	0.528242	8.3:+12498788	None:intergenic
AACTCCGGTGCCGCGTCTCT+TGG	0.359466	8.3:-12498792	MS.gene59958:CDS
AACTTCATTTGTGCCGTTCC+AGG	0.347262	8.3:-12500074	MS.gene59958:CDS
AAGCAGTAGTAGATGGTGCT+GGG	0.527160	8.3:+12499892	None:intergenic
AAGGAGAAGGTGATGTGGCA+GGG	0.559165	8.3:+12498940	None:intergenic
ACAGCTTACGATAGTTGCAA+TGG	0.598738	8.3:-12500164	MS.gene59958:CDS
ACAGGTCCAGTAGCAGGAGC+TGG	0.516512	8.3:+12499855	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAGTAACACTTAACAAAAC+TGG	-	chr8.3:12500168-12500187	MS.gene59958:CDS	30.0%
AGTGTTAGATTTGATTAACC+GGG	-	chr8.3:12499586-12499605	MS.gene59958:intron	30.0%
CAGTGTTAGATTTGATTAAC+CGG	-	chr8.3:12499585-12499604	MS.gene59958:intron	30.0%
TTAACCAGGTTGTTGAATTT+AGG	-	chr8.3:12499920-12499939	MS.gene59958:CDS	30.0%
AGATTTGATTAACCGGGAAA+TGG	-	chr8.3:12499592-12499611	MS.gene59958:intron	35.0%
ATAGATTACACATTACCCTC+TGG	-	chr8.3:12499655-12499674	MS.gene59958:intron	35.0%
ATTTGCACAAACAAGACATG+TGG	-	chr8.3:12498805-12498824	MS.gene59958:CDS	35.0%
CAAAAGCTTTCCATCAATGT+TGG	-	chr8.3:12500237-12500256	MS.gene59958:intron	35.0%
GATACCTAAATTCAACAACC+TGG	+	chr8.3:12499927-12499946	None:intergenic	35.0%
GGTACTCATTACTATATCTG+TGG	-	chr8.3:12500189-12500208	MS.gene59958:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	12498737	12500500	12498737	ID=MS.gene59958
chr8.3	mRNA	12498737	12500500	12498737	ID=MS.gene59958.t1;Parent=MS.gene59958
chr8.3	exon	12500314	12500500	12500314	ID=MS.gene59958.t1.exon1;Parent=MS.gene59958.t1
chr8.3	CDS	12500314	12500500	12500314	ID=cds.MS.gene59958.t1;Parent=MS.gene59958.t1
chr8.3	exon	12499748	12500233	12499748	ID=MS.gene59958.t1.exon2;Parent=MS.gene59958.t1
chr8.3	CDS	12499748	12500233	12499748	ID=cds.MS.gene59958.t1;Parent=MS.gene59958.t1
chr8.3	exon	12498737	12499176	12498737	ID=MS.gene59958.t1.exon3;Parent=MS.gene59958.t1
chr8.3	CDS	12498737	12499176	12498737	ID=cds.MS.gene59958.t1;Parent=MS.gene59958.t1

Gene Sequence

>MS.gene59958

ATGGCAAAGAACCTTAATGTTCTTGTTTTTGTTCTACTTGCAGTTGCTGCAAACCTGTTCCATGGATCATTTGCACAAACAAGACATGTGGTTGGTGATACCACAGGTTGGACTATCCCAACCAACGGTGCTTCATTCTATACCAACTGGGCTTCCAACAAAACCTTCACTGTAGGCGACACTCTCGGTAAGAAACAATATATTTCAGCCCTTGTCATTTTGATTCGTTTATATGAGAATTGTTTTAACGTAAACATGATTTTGCAGTGTTCAATTATGCAAGTGGACAACATGATGTTGCTAAGGTAACAAAAACAGCTTACGATAGTTGCAATGGAGCAAACACACTTTTCACCTTAACAAACAGTCCAGCTACCGTGACATTGAACGAAACAGGACAACAAAACTTCATTTGTGCCGTTCCAGGACATTGTTCAGCTGGACAAAAACTTTCAATTAACGTTGTTAAAGCTTCTACTTCACCTGTTTCTGCTCCAACTCCAAGTGCTTCTCCACCTAAGGCTACTCCAGCTCCTACCCCTGTTCCAGCAAAAGCACCGGCACCAACCAAGGCCGCTACACCTGCCCCAGCACCATCTACTACTGCTTCACCAACACCTGCTCCAGCTCCTGCTACTGGACCTGTTACTTACACCGTCGGTGACACCATTGGCTGGACCATTCCATCTAACGGTGCTGCTGCTTATACAACCTGGGCTTCTCGCAAGTCTTTCAAAGTTGGAGACATCCTTGGTAAGTCATGGTTATTGAGAAATTTATTTATGATTAAATACTAGTATTTAATTTTAAGATGAAAAAAATTAGAGATCTTGTACATGTGATCAATGCAGTGTTAGATTTGATTAACCGGGAAATGGATCATCATACGGTGATTTTCACACAAGAGGCTAATGTGCTATAGATTACACATTACCCTCTGGTGTGAAAGTCACAACACATGAGAGGACCCCATTCTTGATTAATAGGTATATCAATTGTCACATCTTATTAATTTATAATTTTACTAACCACTAATTTGAATATGATTAATTATAATTTTAAGTGTTCAACTTCAAATTTACATTATGATTAACAAAATTCAATACATGGTTAATGAGATATCCGATCTCTTGATTAATATATACTCATCAACTATTTGCTTTAAAAGTGTGTGTGAAATGCATTAACCAGGTTGTTGAATTTAGGTATCTAAAATTGTTGTGGAAATATAGTCACTTTTTTTAAATAACTTGTTTAAAAAAAATATGATTTAGATGTATAATTTTGTGAGGCAAATTATTGATCAACTTGTACTATGATGCAGTTTTCAATTTCCAATTGAATGCACACAACGTGGAAGAAGTGACAAAGGAAAAGTATGATTCATGCAACTCAGCTTCTCCAATTGCAACTTTCAGCAACCCACCAGTGAGAGTAACACTTAACAAAACTGGTACTCATTACTATATCTGTGGTGTTACTGGACACTGCTCTGCAGGCCAAAAGCTTTCCATCAATGTTGGTAGTGGTAGCAGCAGCCCTGCCACATCACCTTCTCCTTCCGCCTCATCGCCTTCTCCTTCCACAGGTGCCACTCCGCCTTCCGCCTCCGGTTCTCCCTCACCGGGGTCTCCTGTCACCCCTTCTTCCCAGTCTCCAGGTGGTTCTGTCAGCCCCCCTCCTGAGAACTCCGGTGCCGCGTCTCTTGGAGTTGCCGGGTTATTTGTCACCGTCCTCTCAATTGCAGCTGCATTTTTCTGTTAG

Protein sequence

>MS.gene59958.t1

MAKNLNVLVFVLLAVAANLFHGSFAQTRHVVGDTTGWTIPTNGASFYTNWASNKTFTVGDTLVFNYASGQHDVAKVTKTAYDSCNGANTLFTLTNSPATVTLNETGQQNFICAVPGHCSAGQKLSINVVKASTSPVSAPTPSASPPKATPAPTPVPAKAPAPTKAATPAPAPSTTASPTPAPAPATGPVTYTVGDTIGWTIPSNGAAAYTTWASRKSFKVGDILVFNFQLNAHNVEEVTKEKYDSCNSASPIATFSNPPVRVTLNKTGTHYYICGVTGHCSAGQKLSINVGSGSSSPATSPSPSASSPSPSTGATPPSASGSPSPGSPVTPSSQSPGGSVSPPPENSGAASLGVAGLFVTVLSIAAAFFC