AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59958

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59958.t1 MTR_4g114870 97.297 370 10 0 1 370 1 370 0.0 704
MS.gene59958.t1 MTR_6g083240 57.225 173 60 4 1 171 1 161 2.34e-53 174
MS.gene59958.t1 MTR_6g083240 48.227 141 66 3 190 327 26 162 2.06e-35 128
MS.gene59958.t1 MTR_0334s0010 46.875 128 65 3 1 128 1 125 2.04e-31 118
MS.gene59958.t1 MTR_0334s0010 47.273 110 56 2 181 290 18 125 7.76e-28 108
MS.gene59958.t1 MTR_5g006040 43.885 139 70 4 29 166 26 157 3.15e-31 117
MS.gene59958.t1 MTR_5g006040 37.931 174 85 4 190 362 25 176 2.21e-30 115
MS.gene59958.t1 MTR_4g067200 44.531 128 66 4 3 128 5 129 1.41e-30 115
MS.gene59958.t1 MTR_4g067200 42.017 119 65 2 185 301 22 138 6.88e-26 102
MS.gene59958.t1 MTR_4g066110 44.531 128 66 4 3 128 5 129 1.41e-30 115
MS.gene59958.t1 MTR_4g066110 42.017 119 65 2 185 301 22 138 6.88e-26 102
MS.gene59958.t1 MTR_1g104800 41.497 147 79 3 190 336 24 163 3.61e-27 106
MS.gene59958.t1 MTR_1g104800 41.096 146 76 3 31 176 27 162 1.88e-25 102
MS.gene59958.t1 MTR_3g099570 44.828 116 59 2 27 142 24 134 1.92e-26 104
MS.gene59958.t1 MTR_3g099570 41.985 131 67 4 180 309 18 140 2.30e-24 99.0
MS.gene59958.t1 MTR_3g099580 49.020 102 47 2 27 128 24 120 9.09e-26 102
MS.gene59958.t1 MTR_3g099580 50.000 100 45 2 191 290 26 120 1.45e-24 99.4
MS.gene59958.t1 MTR_3g099980 48.544 103 48 2 27 128 24 122 6.66e-25 101
MS.gene59958.t1 MTR_3g099980 45.690 116 55 3 176 290 14 122 6.00e-24 98.6
MS.gene59958.t1 MTR_3g099680 48.544 103 48 2 27 128 24 122 7.12e-25 101
MS.gene59958.t1 MTR_3g099680 45.690 116 55 3 176 290 14 122 7.69e-24 98.6
MS.gene59958.t1 MTR_1g090420 34.659 176 106 4 11 181 10 181 2.49e-24 99.8
MS.gene59958.t1 MTR_1g090420 33.333 189 110 6 191 368 28 211 8.39e-23 95.5
MS.gene59958.t1 MTR_8g099220 36.434 129 79 2 1 128 1 127 2.61e-24 97.1
MS.gene59958.t1 MTR_8g099220 36.634 101 63 1 190 290 28 127 2.75e-16 75.1
MS.gene59958.t1 MTR_1g090190 35.526 152 85 3 190 341 22 160 2.30e-23 95.9
MS.gene59958.t1 MTR_1g090190 35.200 125 74 3 27 149 21 140 1.62e-16 77.0
MS.gene59958.t1 MTR_1g014120 40.000 120 67 2 194 311 29 145 5.20e-23 95.1
MS.gene59958.t1 MTR_1g014120 33.333 138 89 2 13 147 9 146 7.51e-19 84.0
MS.gene59958.t1 MTR_8g007020 40.698 172 84 7 1 170 1 156 1.69e-22 94.0
MS.gene59958.t1 MTR_8g007020 34.615 182 91 6 191 365 24 184 9.63e-20 86.3
MS.gene59958.t1 MTR_6g022170 42.574 101 55 2 191 290 34 132 7.28e-22 91.7
MS.gene59958.t1 MTR_6g022170 38.679 106 62 2 25 129 30 133 7.11e-18 80.5
MS.gene59958.t1 MTR_3g099540 41.818 110 59 2 191 300 27 131 8.88e-22 90.1
MS.gene59958.t1 MTR_3g099540 37.963 108 62 2 27 134 25 127 6.24e-15 71.2
MS.gene59958.t1 MTR_8g463180 39.130 115 67 2 191 305 24 135 1.13e-21 94.4
MS.gene59958.t1 MTR_8g463180 29.577 213 112 6 5 189 7 209 5.37e-18 84.0
MS.gene59958.t1 MTR_2g101300 36.913 149 72 6 191 336 27 156 2.15e-21 90.9
MS.gene59958.t1 MTR_2g101300 34.437 151 95 3 11 160 9 156 6.56e-20 87.0
MS.gene59958.t1 MTR_2g088990 35.256 156 93 5 9 163 6 154 3.33e-21 91.3
MS.gene59958.t1 MTR_2g088990 41.964 112 57 3 198 309 33 136 8.53e-21 90.5
MS.gene59958.t1 MTR_4g114830 40.984 122 69 3 25 144 25 145 1.27e-20 88.2
MS.gene59958.t1 MTR_4g114830 40.816 98 55 3 191 285 28 125 2.35e-18 81.6
MS.gene59958.t1 MTR_1g112700 34.884 129 69 3 190 307 32 156 3.31e-20 87.4
MS.gene59958.t1 MTR_1g112700 30.345 145 94 4 11 154 16 154 1.88e-16 77.0
MS.gene59958.t1 MTR_2g043730 40.000 120 61 5 11 127 12 123 4.27e-20 85.5
MS.gene59958.t1 MTR_2g043730 39.394 99 53 2 191 289 32 123 1.21e-16 75.9
MS.gene59958.t1 MTR_7g086140 37.795 127 64 4 9 129 6 123 4.90e-20 86.3
MS.gene59958.t1 MTR_7g086140 40.594 101 55 2 191 290 26 122 6.26e-15 72.0
MS.gene59958.t1 MTR_7g086190 37.795 127 64 4 9 129 6 123 4.90e-20 86.3
MS.gene59958.t1 MTR_7g086190 40.594 101 55 2 191 290 26 122 6.26e-15 72.0
MS.gene59958.t1 MTR_2g079030 34.356 163 94 5 190 343 24 182 1.24e-19 90.9
MS.gene59958.t1 MTR_2g079030 29.949 197 123 8 1 192 1 187 2.58e-13 71.6
MS.gene59958.t1 MTR_1g105130 37.190 121 71 2 11 130 7 123 1.29e-19 85.1
MS.gene59958.t1 MTR_1g105130 41.584 101 54 2 191 290 25 121 5.90e-17 77.8
MS.gene59958.t1 MTR_1g105120 37.190 121 71 2 11 130 7 123 1.29e-19 85.1
MS.gene59958.t1 MTR_1g105120 41.584 101 54 2 191 290 25 121 5.90e-17 77.8
MS.gene59958.t1 MTR_8g007035 43.137 102 51 3 191 290 24 120 2.38e-19 85.5
MS.gene59958.t1 MTR_8g007035 41.935 124 60 5 10 131 10 123 2.50e-18 82.8
MS.gene59958.t1 MTR_7g086090 37.500 120 71 1 9 128 6 121 4.40e-19 85.9
MS.gene59958.t1 MTR_7g086090 36.275 102 57 3 191 290 26 121 5.49e-13 68.6
MS.gene59958.t1 MTR_7g086160 37.500 128 64 5 9 129 6 124 5.33e-19 83.6
MS.gene59958.t1 MTR_7g086160 38.261 115 66 2 177 290 13 123 2.45e-16 76.3
MS.gene59958.t1 MTR_4g111640 32.394 142 85 4 191 330 32 164 6.67e-19 84.0
MS.gene59958.t1 MTR_4g111640 32.000 150 87 4 27 174 30 166 2.16e-16 77.0
MS.gene59958.t1 MTR_8g088830 41.584 101 51 3 190 289 27 120 7.15e-19 82.0
MS.gene59958.t1 MTR_8g088830 42.268 97 50 1 31 127 30 120 3.43e-18 80.1
MS.gene59958.t1 MTR_4g081100 38.235 102 63 0 191 292 32 133 9.08e-19 84.3
MS.gene59958.t1 MTR_4g081100 32.000 100 68 0 30 129 33 132 1.26e-13 70.1
MS.gene59958.t1 MTR_3g105930 36.607 112 70 1 194 304 31 142 2.27e-18 82.4
MS.gene59958.t1 MTR_3g105930 28.916 166 96 3 25 190 24 167 9.11e-14 69.3
MS.gene59958.t1 MTR_2g090580 31.953 169 108 4 191 355 27 192 2.40e-18 82.8
MS.gene59958.t1 MTR_2g090580 34.615 156 93 4 31 185 29 176 5.75e-18 81.6
MS.gene59958.t1 MTR_7g086220 35.484 124 65 4 12 129 8 122 6.52e-18 80.5
MS.gene59958.t1 MTR_7g086220 37.624 101 58 2 191 290 25 121 5.68e-14 69.3
MS.gene59958.t1 MTR_7g086280 31.737 167 97 4 10 164 7 168 8.04e-18 80.9
MS.gene59958.t1 MTR_7g086280 34.000 100 62 1 191 290 26 121 1.55e-12 65.9
MS.gene59958.t1 MTR_7g086230 32.121 165 95 4 12 164 9 168 2.05e-17 79.7
MS.gene59958.t1 MTR_7g086230 34.000 100 62 1 191 290 26 121 1.60e-12 65.9
MS.gene59958.t1 MTR_4g124280 31.548 168 108 2 190 357 24 184 2.23e-17 80.9
MS.gene59958.t1 MTR_4g124280 33.758 157 97 3 31 186 27 177 4.04e-17 80.1
MS.gene59958.t1 MTR_4g077787 31.111 135 87 3 191 323 32 162 4.32e-17 79.0
MS.gene59958.t1 MTR_4g077787 30.645 124 80 3 27 148 30 149 1.48e-13 68.9
MS.gene59958.t1 MTR_8g089110 40.000 100 53 2 190 289 27 119 5.58e-17 76.6
MS.gene59958.t1 MTR_8g089110 35.537 121 67 3 7 127 10 119 1.05e-14 70.5
MS.gene59958.t1 MTR_3g089035 37.736 106 59 2 183 288 25 123 6.22e-17 76.6
MS.gene59958.t1 MTR_3g089035 37.624 101 56 2 29 129 33 126 1.30e-14 70.5
MS.gene59958.t1 MTR_7g086100 35.593 118 72 1 12 129 9 122 1.11e-16 79.0
MS.gene59958.t1 MTR_7g086100 37.255 102 56 3 191 290 26 121 9.74e-14 70.5
MS.gene59958.t1 MTR_6g023760 39.423 104 57 4 191 293 26 124 1.58e-16 77.0
MS.gene59958.t1 MTR_6g023760 36.893 103 59 3 27 128 24 121 1.93e-13 68.6
MS.gene59958.t1 MTR_8g095013 33.750 160 92 3 191 337 25 183 2.13e-16 79.3
MS.gene59958.t1 MTR_8g095013 28.986 207 118 6 1 188 1 197 1.87e-15 76.6
MS.gene59958.t1 MTR_8g086360 33.117 154 86 3 31 184 26 162 3.68e-16 76.6
MS.gene59958.t1 MTR_8g086360 35.772 123 75 2 184 304 17 137 3.79e-16 76.3
MS.gene59958.t1 MTR_2g090575 33.621 116 70 2 191 301 25 138 5.57e-16 79.0
MS.gene59958.t1 MTR_2g090575 29.130 230 131 7 5 213 10 228 1.41e-15 77.4
MS.gene59958.t1 MTR_6g080660 36.697 109 64 2 191 299 28 131 6.67e-16 76.3
MS.gene59958.t1 MTR_6g080660 32.203 118 75 2 11 128 10 122 4.09e-15 73.9
MS.gene59958.t1 MTR_4g078410 34.694 147 90 4 31 177 27 167 6.88e-16 75.5
MS.gene59958.t1 MTR_4g078410 31.538 130 84 3 190 318 24 149 1.03e-13 69.3
MS.gene59958.t1 MTR_6g013420 35.606 132 78 3 184 313 31 157 7.30e-16 75.5
MS.gene59958.t1 MTR_6g013420 35.252 139 83 3 8 144 17 150 2.03e-14 71.2
MS.gene59958.t1 MTR_4g112310 32.787 122 73 3 209 330 2 114 1.99e-15 72.8
MS.gene59958.t1 MTR_4g112310 31.452 124 72 3 47 170 2 112 2.75e-12 63.9
MS.gene59958.t1 MTR_8g095020 31.250 192 112 5 31 208 27 212 5.56e-15 75.5
MS.gene59958.t1 MTR_8g095020 33.108 148 83 4 191 325 25 169 3.23e-13 70.1
MS.gene59958.t1 MTR_2g083250 36.275 102 63 2 191 290 26 127 6.16e-15 74.3
MS.gene59958.t1 MTR_2g083250 33.945 109 70 2 28 134 25 133 5.19e-13 68.9
MS.gene59958.t1 MTR_8g094990 34.507 142 86 2 191 326 25 165 1.42e-14 73.6
MS.gene59958.t1 MTR_8g094990 30.102 196 124 5 1 188 1 191 9.50e-13 68.2
MS.gene59958.t1 MTR_2g043790 38.384 99 54 2 191 289 7 98 1.74e-14 69.3
MS.gene59958.t1 MTR_2g043790 36.190 105 59 3 23 127 2 98 5.89e-13 64.7
MS.gene59958.t1 MTR_6g013200 32.877 146 91 3 184 327 28 168 3.88e-14 70.1
MS.gene59958.t1 MTR_6g013200 30.263 152 91 4 9 158 23 161 4.86e-12 64.3
MS.gene59958.t1 MTR_6g013170 35.938 128 73 4 187 313 27 146 8.24e-14 69.3
MS.gene59958.t1 MTR_3g089005 36.449 107 61 2 183 289 25 124 8.45e-14 68.2
MS.gene59958.t1 MTR_3g089005 31.746 126 72 4 5 127 10 124 1.28e-12 64.7
MS.gene59958.t1 MTR_1g077790 33.562 146 88 3 191 327 29 174 1.64e-13 68.9
MS.gene59958.t1 MTR_1g077790 33.333 99 65 1 31 128 31 129 1.68e-12 66.2
MS.gene59958.t1 MTR_2g025580 28.758 153 93 3 9 160 7 144 6.22e-13 66.6
MS.gene59958.t1 MTR_2g025580 29.530 149 95 3 185 332 21 160 2.41e-11 62.4
MS.gene59958.t1 MTR_4g007250 34.375 128 76 3 182 309 23 142 7.13e-13 66.6
MS.gene59958.t1 MTR_4g007250 34.314 102 62 2 27 128 27 123 3.17e-12 64.7
MS.gene59958.t1 MTR_4g130780 30.693 101 69 1 191 290 29 129 1.02e-12 66.6
MS.gene59958.t1 MTR_3g092170 26.289 194 127 3 1 184 1 188 2.33e-11 64.3
MS.gene59958.t1 MTR_5g088990 32.051 156 86 6 191 329 34 186 5.05e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59958.t1 AT2G32300 41.497 147 82 2 9 155 6 148 2.06e-30 117
MS.gene59958.t1 AT2G32300 39.716 141 78 2 191 331 26 159 7.59e-24 99.8
MS.gene59958.t1 AT2G32300 41.497 147 82 2 9 155 36 178 7.49e-30 116
MS.gene59958.t1 AT2G32300 39.716 141 78 2 191 331 56 189 2.43e-23 99.0
MS.gene59958.t1 AT2G31050 39.655 174 96 2 10 177 10 180 2.07e-29 112
MS.gene59958.t1 AT2G31050 42.017 119 66 1 185 303 23 138 5.83e-25 100
MS.gene59958.t1 AT5G20230 46.721 122 63 2 31 151 27 147 1.92e-28 110
MS.gene59958.t1 AT5G20230 37.430 179 105 3 191 369 25 196 3.28e-28 109
MS.gene59958.t1 AT3G17675 51.923 104 42 3 27 128 30 127 5.12e-28 107
MS.gene59958.t1 AT3G17675 40.000 100 56 1 191 290 32 127 2.02e-20 86.7
MS.gene59958.t1 AT2G26720 40.426 141 81 1 1 141 1 138 2.32e-27 107
MS.gene59958.t1 AT2G26720 38.655 119 70 1 185 303 23 138 1.73e-22 94.4
MS.gene59958.t1 AT2G25060 42.149 121 61 2 192 311 32 144 1.27e-25 102
MS.gene59958.t1 AT2G25060 39.241 158 77 4 13 163 14 159 2.21e-23 96.3
MS.gene59958.t1 AT5G26330 46.000 100 52 1 191 290 24 121 1.43e-25 102
MS.gene59958.t1 AT5G26330 45.455 99 52 1 31 129 26 122 2.74e-22 93.6
MS.gene59958.t1 AT1G45063 26.780 295 169 6 9 301 9 258 1.59e-25 104
MS.gene59958.t1 AT1G45063 26.780 295 169 6 9 301 9 258 9.55e-25 104
MS.gene59958.t1 AT3G20570 38.281 128 78 1 191 318 30 156 1.42e-23 97.4
MS.gene59958.t1 AT3G20570 34.239 184 117 4 1 183 1 181 2.08e-20 88.6
MS.gene59958.t1 AT3G53330 27.698 278 165 9 31 290 27 286 5.46e-23 98.2
MS.gene59958.t1 AT4G31840 34.807 181 84 6 192 369 28 177 8.71e-23 94.4
MS.gene59958.t1 AT4G31840 31.613 155 98 1 6 160 5 151 5.49e-19 84.0
MS.gene59958.t1 AT5G53870 38.235 170 86 5 191 347 22 185 1.18e-21 95.5
MS.gene59958.t1 AT5G53870 34.066 182 106 4 10 188 12 182 2.52e-19 88.6
MS.gene59958.t1 AT5G53870 38.235 170 86 5 191 347 22 185 1.47e-21 95.1
MS.gene59958.t1 AT5G53870 34.066 182 106 4 10 188 12 182 3.29e-19 88.6
MS.gene59958.t1 AT3G60270 41.121 107 58 2 184 290 19 120 1.77e-21 91.3
MS.gene59958.t1 AT3G60270 42.574 101 53 2 31 131 28 123 1.90e-19 85.5
MS.gene59958.t1 AT5G07475 38.571 140 71 5 190 319 29 163 2.97e-21 90.9
MS.gene59958.t1 AT5G07475 38.333 120 67 3 27 144 28 142 2.72e-17 79.7
MS.gene59958.t1 AT4G28365 31.053 190 113 6 1 188 5 178 1.38e-20 89.0
MS.gene59958.t1 AT4G28365 34.737 190 93 8 191 368 29 199 3.99e-19 85.1
MS.gene59958.t1 AT4G30590 34.579 107 69 1 185 290 22 128 9.10e-20 86.7
MS.gene59958.t1 AT4G30590 33.803 142 87 3 8 145 10 148 6.88e-16 75.9
MS.gene59958.t1 AT1G79800 37.838 111 66 2 181 290 24 132 3.90e-19 84.7
MS.gene59958.t1 AT1G79800 34.454 119 71 4 31 145 36 151 3.30e-14 70.9
MS.gene59958.t1 AT5G15350 38.519 135 76 3 181 313 19 148 4.33e-19 84.3
MS.gene59958.t1 AT5G15350 33.333 117 71 3 28 142 28 139 7.64e-12 63.9
MS.gene59958.t1 AT3G27200 35.878 131 71 5 1 129 3 122 6.41e-18 80.9
MS.gene59958.t1 AT3G27200 36.634 101 58 3 191 290 26 121 9.33e-16 75.1
MS.gene59958.t1 AT1G72230 34.328 134 83 2 197 330 29 157 9.90e-18 80.5
MS.gene59958.t1 AT1G72230 31.111 180 107 4 4 183 3 165 3.01e-17 79.3
MS.gene59958.t1 AT3G60280 38.679 106 60 3 183 288 16 116 3.99e-17 80.1
MS.gene59958.t1 AT3G60280 37.624 101 58 2 31 131 26 121 3.99e-14 71.2
MS.gene59958.t1 AT5G25090 32.544 169 101 4 194 362 30 185 5.03e-17 79.0
MS.gene59958.t1 AT5G25090 36.735 98 62 0 32 129 30 127 7.24e-13 67.0
MS.gene59958.t1 AT5G14345 33.636 110 70 2 197 305 22 129 6.36e-17 77.4
MS.gene59958.t1 AT5G14345 32.110 109 71 2 36 143 23 129 3.90e-13 66.6
MS.gene59958.t1 AT2G44790 37.719 114 60 4 184 295 25 129 1.34e-16 78.2
MS.gene59958.t1 AT2G44790 40.909 88 49 2 47 132 42 128 2.14e-15 74.7
MS.gene59958.t1 AT1G64640 36.667 120 74 2 191 309 33 151 2.71e-16 77.0
MS.gene59958.t1 AT1G64640 31.081 148 94 3 8 153 10 151 1.28e-15 75.1
MS.gene59958.t1 AT4G27520 33.333 144 91 4 3 146 7 145 4.88e-16 79.0
MS.gene59958.t1 AT4G27520 34.884 129 79 3 191 317 31 156 4.88e-16 79.0
MS.gene59958.t1 AT1G22480 40.385 104 57 2 34 137 24 122 2.95e-15 73.6
MS.gene59958.t1 AT1G22480 39.000 100 56 2 196 295 24 118 2.05e-14 71.2
MS.gene59958.t1 AT1G48940 36.842 95 56 2 198 290 33 125 9.24e-15 72.0
MS.gene59958.t1 AT1G48940 31.200 125 81 3 11 132 7 129 1.94e-11 62.8
MS.gene59958.t1 AT3G18590 35.294 102 62 2 191 290 27 126 9.39e-15 72.4
MS.gene59958.t1 AT3G18590 32.000 125 78 4 8 129 7 127 3.66e-12 65.1
MS.gene59958.t1 AT5G57920 29.348 92 65 0 199 290 32 123 3.30e-14 70.9
MS.gene59958.t1 AT5G57920 27.193 114 81 1 30 141 25 138 2.05e-11 62.8
MS.gene59958.t1 AT5G57920 28.696 115 80 1 199 311 31 145 5.75e-14 70.1
MS.gene59958.t1 AT5G57920 27.193 114 81 1 30 141 24 137 5.57e-11 61.6
MS.gene59958.t1 AT2G23990 31.959 194 124 5 8 199 7 194 6.09e-14 70.5
MS.gene59958.t1 AT2G23990 32.000 100 67 1 194 292 31 130 1.12e-13 69.7
MS.gene59958.t1 AT4G12880 36.975 119 68 3 174 290 12 125 7.88e-14 68.6
MS.gene59958.t1 AT5G57920 27.642 123 87 1 199 319 64 186 9.81e-14 70.1
MS.gene59958.t1 AT5G57920 26.786 112 80 1 32 141 59 170 8.39e-11 61.6
MS.gene59958.t1 AT2G02850 34.343 99 58 2 190 288 35 126 2.40e-13 67.0
MS.gene59958.t1 AT2G02850 32.787 122 74 3 9 129 15 129 3.66e-13 66.2
MS.gene59958.t1 AT4G32490 30.818 159 97 5 31 188 33 179 3.80e-13 68.6
MS.gene59958.t1 AT4G32490 28.272 191 102 7 191 355 31 212 1.37e-12 67.0
MS.gene59958.t1 AT1G08500 34.532 139 72 5 191 311 30 167 8.10e-12 64.7
MS.gene59958.t1 AT4G01380 35.417 96 59 3 198 291 79 173 1.35e-11 63.9

Find 127 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTATATCTGTGGTGTTAC+TGG 0.237614 8.3:-12499017 MS.gene59958:CDS
CAACATTGATGGAAAGCTTT+TGG 0.291973 8.3:+12498979 None:intergenic
GCCGCGTCTCTTGGAGTTGC+CGG 0.310336 8.3:-12498783 MS.gene59958:CDS
AACTTCATTTGTGCCGTTCC+AGG 0.347262 8.3:-12500074 MS.gene59958:CDS
CCACGTTGTGTGCATTCAAT+TGG 0.357168 8.3:+12499144 None:intergenic
AACTCCGGTGCCGCGTCTCT+TGG 0.359466 8.3:-12498792 MS.gene59958:CDS
TGGGGCAGGTGTAGCGGCCT+TGG 0.370641 8.3:+12499911 None:intergenic
CGCCTCCGGTTCTCCCTCAC+CGG 0.374150 8.3:-12498874 MS.gene59958:CDS
AGAGTAACACTTAACAAAAC+TGG 0.383012 8.3:-12499047 MS.gene59958:CDS
TGACAGAACCACCTGGAGAC+TGG 0.395337 8.3:+12498829 None:intergenic
GCCACTCCGCCTTCCGCCTC+CGG 0.397842 8.3:-12498888 MS.gene59958:CDS
GTGCTTCATTCTATACCAAC+TGG 0.401088 8.3:-12500351 MS.gene59958:CDS
ACCCCTTCTTCCCAGTCTCC+AGG 0.402610 8.3:-12498840 MS.gene59958:CDS
TCGTTCAATGTCACGGTAGC+TGG 0.403067 8.3:+12500110 None:intergenic
TCACCGACGGTGTAAGTAAC+AGG 0.416106 8.3:+12499837 None:intergenic
TAAGCAGCAGCACCGTTAGA+TGG 0.418533 8.3:+12499795 None:intergenic
GAAGGAGAAGGTGATGTGGC+AGG 0.422952 8.3:+12498939 None:intergenic
TCTCGCAAGTCTTTCAAAGT+TGG 0.423148 8.3:-12499759 MS.gene59958:CDS
AGACCCCGGTGAGGGAGAAC+CGG 0.423247 8.3:+12498869 None:intergenic
GACTGGGAAGAAGGGGTGAC+AGG 0.424292 8.3:+12498846 None:intergenic
TTGTTAAGTGTTACTCTCAC+TGG 0.424897 8.3:+12499053 None:intergenic
TGTCCAGCTGAACAATGTCC+TGG 0.428453 8.3:+12500056 None:intergenic
ACCGTGACATTGAACGAAAC+AGG 0.440151 8.3:-12500104 MS.gene59958:CDS
CTTGGAGTTGGAGCAGAAAC+AGG 0.440341 8.3:+12499996 None:intergenic
CCTGCTCCAGCTCCTGCTAC+TGG 0.441017 8.3:-12499861 MS.gene59958:CDS
GTGCAAATGATCCATGGAAC+AGG 0.441402 8.3:+12500425 None:intergenic
GCTACCACTACCAACATTGA+TGG 0.445225 8.3:+12498968 None:intergenic
AGAATGAAGCACCGTTGGTT+GGG 0.451694 8.3:+12500362 None:intergenic
GCACCGGAGTTCTCAGGAGG+GGG 0.455438 8.3:+12498804 None:intergenic
GGTGGAGAAGCACTTGGAGT+TGG 0.455723 8.3:+12499984 None:intergenic
GTAGGAGCTGGAGTAGCCTT+AGG 0.459132 8.3:+12499963 None:intergenic
GTTACTGGACACTGCTCTGC+AGG 0.461094 8.3:-12499002 MS.gene59958:CDS
GCAGGTGTAGCGGCCTTGGT+TGG 0.463363 8.3:+12499915 None:intergenic
GCCTCCGGTTCTCCCTCACC+GGG 0.464351 8.3:-12498873 MS.gene59958:CDS
GACGCGGCACCGGAGTTCTC+AGG 0.465593 8.3:+12498798 None:intergenic
AACATTAAGGTTCTTTGCCA+TGG 0.467663 8.3:+12500480 None:intergenic
CCACCTGGAGACTGGGAAGA+AGG 0.472672 8.3:+12498837 None:intergenic
GTAGTAGATGGTGCTGGGGC+AGG 0.476078 8.3:+12499897 None:intergenic
CCGCGTCTCTTGGAGTTGCC+GGG 0.476568 8.3:-12498782 MS.gene59958:CDS
GAAAGACTTGCGAGAAGCCC+AGG 0.480465 8.3:+12499767 None:intergenic
GCTGGAACAGGGGTAGGAGC+TGG 0.482904 8.3:+12499951 None:intergenic
TCATCGCCTTCTCCTTCCAC+AGG 0.484678 8.3:-12498912 MS.gene59958:CDS
CCGTCGGTGACACCATTGGC+TGG 0.488830 8.3:-12499824 MS.gene59958:CDS
TAAGTAACAGGTCCAGTAGC+AGG 0.490680 8.3:+12499849 None:intergenic
GACAGAACCACCTGGAGACT+GGG 0.492609 8.3:+12498830 None:intergenic
TTCAAAGTTGGAGACATCCT+TGG 0.494707 8.3:-12499747 MS.gene59958:intron
GTTCCAGGACATTGTTCAGC+TGG 0.499514 8.3:-12500059 MS.gene59958:CDS
TCCTGTTTCGTTCAATGTCA+CGG 0.504654 8.3:+12500103 None:intergenic
GAGGGAGAACCGGAGGCGGA+AGG 0.504963 8.3:+12498879 None:intergenic
TAGAATGAAGCACCGTTGGT+TGG 0.509679 8.3:+12500361 None:intergenic
CACCTGGAGACTGGGAAGAA+GGG 0.510075 8.3:+12498838 None:intergenic
CTTTCCATCAATGTTGGTAG+TGG 0.510316 8.3:-12498972 MS.gene59958:CDS
GGCGATGAGGCGGAAGGAGA+AGG 0.513509 8.3:+12498927 None:intergenic
GTAGCGGCCTTGGTTGGTGC+CGG 0.513837 8.3:+12499921 None:intergenic
GGTAGCTGGACTGTTTGTTA+AGG 0.513870 8.3:+12500124 None:intergenic
GTGCTGCTGCTTATACAACC+TGG 0.514286 8.3:-12499785 MS.gene59958:CDS
CCAGTAGCAGGAGCTGGAGC+AGG 0.514710 8.3:+12499861 None:intergenic
ACAGGTCCAGTAGCAGGAGC+TGG 0.516512 8.3:+12499855 None:intergenic
AGCAGCACCGTTAGATGGAA+TGG 0.523720 8.3:+12499800 None:intergenic
GAAGCAGTAGTAGATGGTGC+TGG 0.524239 8.3:+12499891 None:intergenic
TCCAAGTGCTTCTCCACCTA+AGG 0.524590 8.3:-12499979 MS.gene59958:CDS
GGCGGAGTGGCACCTGTGGA+AGG 0.524949 8.3:+12498900 None:intergenic
GTGGCACCTGTGGAAGGAGA+AGG 0.526612 8.3:+12498906 None:intergenic
AAGCAGTAGTAGATGGTGCT+GGG 0.527160 8.3:+12499892 None:intergenic
CAAAAGCTTTCCATCAATGT+TGG 0.527473 8.3:-12498978 MS.gene59958:CDS
AACTCCAAGAGACGCGGCAC+CGG 0.528242 8.3:+12498788 None:intergenic
GTGACAGGAGACCCCGGTGA+GGG 0.529596 8.3:+12498861 None:intergenic
CGGTGAGGGAGAACCGGAGG+CGG 0.533082 8.3:+12498875 None:intergenic
TGCTGCTGCTTATACAACCT+GGG 0.535411 8.3:-12499784 MS.gene59958:CDS
GGGTTGCTGAAAGTTGCAAT+TGG 0.537641 8.3:+12499077 None:intergenic
GGTATAGAATGAAGCACCGT+TGG 0.538174 8.3:+12500357 None:intergenic
AAAAGCACCGGCACCAACCA+AGG 0.541334 8.3:-12499928 MS.gene59958:CDS
TGGTGCTGGGGCAGGTGTAG+CGG 0.541610 8.3:+12499905 None:intergenic
GCAGGAGCTGGAGCAGGTGT+TGG 0.542881 8.3:+12499867 None:intergenic
TACACCGTCGGTGACACCAT+TGG 0.546097 8.3:-12499828 MS.gene59958:CDS
TTAAGTGTTACTCTCACTGG+TGG 0.550431 8.3:+12499056 None:intergenic
GGAGAAGGCGATGAGGCGGA+AGG 0.552275 8.3:+12498921 None:intergenic
GGAGAACCGGAGGCGGAAGG+CGG 0.556109 8.3:+12498882 None:intergenic
ACCTGGAGACTGGGAAGAAG+GGG 0.556112 8.3:+12498839 None:intergenic
GCCTTAGGTGGAGAAGCACT+TGG 0.556300 8.3:+12499978 None:intergenic
GCAGTGTTCAATTATGCAAG+TGG 0.556563 8.3:-12500215 MS.gene59958:intron
AAGGAGAAGGTGATGTGGCA+GGG 0.559165 8.3:+12498940 None:intergenic
GTTGGTGAAGCAGTAGTAGA+TGG 0.562949 8.3:+12499885 None:intergenic
TGGACAACATGATGTTGCTA+AGG 0.564994 8.3:-12500195 MS.gene59958:CDS
CCCCGGTGAGGGAGAACCGG+AGG 0.568850 8.3:+12498872 None:intergenic
TGCAGCTGCAATTGAGAGGA+CGG 0.569204 8.3:+12498749 None:intergenic
AGATGGAATGGTCCAGCCAA+TGG 0.572806 8.3:+12499812 None:intergenic
GGCACCGGAGTTCTCAGGAG+GGG 0.576346 8.3:+12498803 None:intergenic
TTGTTTGTGCAAATGATCCA+TGG 0.576582 8.3:+12500419 None:intergenic
GGCTGGACCATTCCATCTAA+CGG 0.581650 8.3:-12499807 MS.gene59958:CDS
CCAGCCAATGGTGTCACCGA+CGG 0.581738 8.3:+12499824 None:intergenic
CCCTGTTCCAGCAAAAGCAC+CGG 0.587423 8.3:-12499940 MS.gene59958:CDS
CACCGGAGTTCTCAGGAGGG+GGG 0.588751 8.3:+12498805 None:intergenic
GAAGGGGTGACAGGAGACCC+CGG 0.590847 8.3:+12498855 None:intergenic
TGCTTCATTCTATACCAACT+GGG 0.590943 8.3:-12500350 MS.gene59958:CDS
ACCGGAGGCGGAAGGCGGAG+TGG 0.594091 8.3:+12498887 None:intergenic
ACAGCTTACGATAGTTGCAA+TGG 0.598738 8.3:-12500164 MS.gene59958:CDS
GCGGCACCGGAGTTCTCAGG+AGG 0.599451 8.3:+12498801 None:intergenic
GTTGCTGCAAACCTGTTCCA+TGG 0.601439 8.3:-12500436 MS.gene59958:CDS
TAAGTGTTACTCTCACTGGT+GGG 0.601887 8.3:+12499057 None:intergenic
TTCACTGTAGGCGACACTCT+CGG 0.604689 8.3:-12500313 MS.gene59958:intron
AGCTGAACAATGTCCTGGAA+CGG 0.608366 8.3:+12500061 None:intergenic
GGACCTGTTACTTACACCGT+CGG 0.610193 8.3:-12499840 MS.gene59958:CDS
GGAGCTGGAGTAGCCTTAGG+TGG 0.618274 8.3:+12499966 None:intergenic
CCTTCTTCCCAGTCTCCAGG+TGG 0.619545 8.3:-12498837 MS.gene59958:CDS
GGAAGGCGGAGTGGCACCTG+TGG 0.619926 8.3:+12498896 None:intergenic
CGGCACCGGAGTTCTCAGGA+GGG 0.620355 8.3:+12498802 None:intergenic
AAAATGCAGCTGCAATTGAG+AGG 0.622849 8.3:+12498745 None:intergenic
GGAAGGAGAAGGCGATGAGG+CGG 0.623870 8.3:+12498917 None:intergenic
GAGAGTGTCGCCTACAGTGA+AGG 0.626729 8.3:+12500315 None:intergenic
GGTACTCATTACTATATCTG+TGG 0.633939 8.3:-12499026 MS.gene59958:CDS
CCCGGCAACTCCAAGAGACG+CGG 0.637522 8.3:+12498782 None:intergenic
GAGGACGGTGACAAATAACC+CGG 0.639473 8.3:+12498764 None:intergenic
TCCAACAAAACCTTCACTGT+AGG 0.644441 8.3:-12500325 MS.gene59958:CDS
CCAATTGAATGCACACAACG+TGG 0.647358 8.3:-12499144 MS.gene59958:CDS
ATTTGCACAAACAAGACATG+TGG 0.650443 8.3:-12500410 MS.gene59958:CDS
GGTGACAGGAGACCCCGGTG+AGG 0.653268 8.3:+12498860 None:intergenic
CATGTGGTTGGTGATACCAC+AGG 0.660891 8.3:-12500394 MS.gene59958:CDS
GGCGGAAGGAGAAGGTGATG+TGG 0.668642 8.3:+12498935 None:intergenic
CAACGTGGAAGAAGTGACAA+AGG 0.672668 8.3:-12499129 MS.gene59958:CDS
GGTTGGACTATCCCAACCAA+CGG 0.674561 8.3:-12500373 MS.gene59958:CDS
AGCAGTAGTAGATGGTGCTG+GGG 0.674917 8.3:+12499893 None:intergenic
TGTGGAAGGAGAAGGCGATG+AGG 0.677050 8.3:+12498914 None:intergenic
GCACAAACAAGACATGTGGT+TGG 0.685878 8.3:-12500406 MS.gene59958:CDS
GGTTGGGATAGTCCAACCTG+TGG 0.687809 8.3:+12500378 None:intergenic
TGGTTGGTGATACCACAGGT+TGG 0.725223 8.3:-12500390 MS.gene59958:CDS
CCTCCGGTTCTCCCTCACCG+GGG 0.753255 8.3:-12498872 MS.gene59958:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATCATATTCAAATTAG+TGG + chr8.3:12499768-12499787 None:intergenic 15.0%
!! ATATACCTATTAATCAAGAA+TGG + chr8.3:12499709-12499728 None:intergenic 20.0%
!! GATTAACAAAATTCAATACA+TGG - chr8.3:12499824-12499843 MS.gene59958:CDS 20.0%
!! GTATATATTAATCAAGAGAT+CGG + chr8.3:12499862-12499881 None:intergenic 20.0%
!! TATACCTATTAATCAAGAAT+GGG + chr8.3:12499708-12499727 None:intergenic 20.0%
!!! TTTAGATGTATAATTTTGTG+AGG - chr8.3:12500006-12500025 MS.gene59958:CDS 20.0%
! ATAAACGAATCAAAATGACA+AGG + chr8.3:12498949-12498968 None:intergenic 25.0%
! ATACCTATTAATCAAGAATG+GGG + chr8.3:12499707-12499726 None:intergenic 25.0%
! GAACAAAAACAAGAACATTA+AGG + chr8.3:12498751-12498770 None:intergenic 25.0%
! TAAACGAATCAAAATGACAA+GGG + chr8.3:12498948-12498967 None:intergenic 25.0%
!! TTAGGTATCTAAAATTGTTG+TGG - chr8.3:12499938-12499957 MS.gene59958:CDS 25.0%
AGAGTAACACTTAACAAAAC+TGG - chr8.3:12500168-12500187 MS.gene59958:CDS 30.0%
AGTGTTAGATTTGATTAACC+GGG - chr8.3:12499586-12499605 MS.gene59958:intron 30.0%
CAGTGTTAGATTTGATTAAC+CGG - chr8.3:12499585-12499604 MS.gene59958:intron 30.0%
TTAACCAGGTTGTTGAATTT+AGG - chr8.3:12499920-12499939 MS.gene59958:CDS 30.0%
AGATTTGATTAACCGGGAAA+TGG - chr8.3:12499592-12499611 MS.gene59958:intron 35.0%
ATAGATTACACATTACCCTC+TGG - chr8.3:12499655-12499674 MS.gene59958:intron 35.0%
ATTTGCACAAACAAGACATG+TGG - chr8.3:12498805-12498824 MS.gene59958:CDS 35.0%
CAAAAGCTTTCCATCAATGT+TGG - chr8.3:12500237-12500256 MS.gene59958:intron 35.0%
GATACCTAAATTCAACAACC+TGG + chr8.3:12499927-12499946 None:intergenic 35.0%
GGTACTCATTACTATATCTG+TGG - chr8.3:12500189-12500208 MS.gene59958:CDS 35.0%
TCAATAACCATGACTTACCA+AGG + chr8.3:12499488-12499507 None:intergenic 35.0%
TGCTTCATTCTATACCAACT+GGG - chr8.3:12498865-12498884 MS.gene59958:CDS 35.0%
TTGTTTGTGCAAATGATCCA+TGG + chr8.3:12498799-12498818 None:intergenic 35.0%
! CAACATTGATGGAAAGCTTT+TGG + chr8.3:12500239-12500258 None:intergenic 35.0%
! TTGTTAAGTGTTACTCTCAC+TGG + chr8.3:12500165-12500184 None:intergenic 35.0%
!! TACTATATCTGTGGTGTTAC+TGG - chr8.3:12500198-12500217 MS.gene59958:CDS 35.0%
AAAATGCAGCTGCAATTGAG+AGG + chr8.3:12500473-12500492 None:intergenic 40.0%
ACAGCTTACGATAGTTGCAA+TGG - chr8.3:12499051-12499070 MS.gene59958:CDS 40.0%
GCAGTGTTCAATTATGCAAG+TGG - chr8.3:12499000-12499019 MS.gene59958:CDS 40.0%
GGACCCCATTCTTGATTAAT+AGG - chr8.3:12499701-12499720 MS.gene59958:intron 40.0%
GTGCTTCATTCTATACCAAC+TGG - chr8.3:12498864-12498883 MS.gene59958:CDS 40.0%
GTGTGTGAAATGCATTAACC+AGG - chr8.3:12499906-12499925 MS.gene59958:CDS 40.0%
TCCAACAAAACCTTCACTGT+AGG - chr8.3:12498890-12498909 MS.gene59958:CDS 40.0%
TCCTGTTTCGTTCAATGTCA+CGG + chr8.3:12499115-12499134 None:intergenic 40.0%
TCTCGCAAGTCTTTCAAAGT+TGG - chr8.3:12499456-12499475 MS.gene59958:intron 40.0%
TGAAAGTCACAACACATGAG+AGG - chr8.3:12499680-12499699 MS.gene59958:intron 40.0%
TGGACAACATGATGTTGCTA+AGG - chr8.3:12499020-12499039 MS.gene59958:CDS 40.0%
TTCAAAGTTGGAGACATCCT+TGG - chr8.3:12499468-12499487 MS.gene59958:intron 40.0%
! CTTTCCATCAATGTTGGTAG+TGG - chr8.3:12500243-12500262 MS.gene59958:intron 40.0%
! TAAGTGTTACTCTCACTGGT+GGG + chr8.3:12500161-12500180 None:intergenic 40.0%
! TACGGTGATTTTCACACAAG+AGG - chr8.3:12499622-12499641 MS.gene59958:intron 40.0%
! TTAAGTGTTACTCTCACTGG+TGG + chr8.3:12500162-12500181 None:intergenic 40.0%
AAGCAGTAGTAGATGGTGCT+GGG + chr8.3:12499326-12499345 None:intergenic 45.0%
ACCGTGACATTGAACGAAAC+AGG - chr8.3:12499111-12499130 MS.gene59958:CDS 45.0%
AGCTGAACAATGTCCTGGAA+CGG + chr8.3:12499157-12499176 None:intergenic 45.0%
CAACGTGGAAGAAGTGACAA+AGG - chr8.3:12500086-12500105 MS.gene59958:CDS 45.0%
CCAATTGAATGCACACAACG+TGG - chr8.3:12500071-12500090 MS.gene59958:CDS 45.0%
CCACGTTGTGTGCATTCAAT+TGG + chr8.3:12500074-12500093 None:intergenic 45.0%
CCGGGAAATGGATCATCATA+CGG - chr8.3:12499604-12499623 MS.gene59958:intron 45.0%
CCGTATGATGATCCATTTCC+CGG + chr8.3:12499607-12499626 None:intergenic 45.0%
GAGACATCCTTGGTAAGTCA+TGG - chr8.3:12499478-12499497 MS.gene59958:intron 45.0%
GCACAAACAAGACATGTGGT+TGG - chr8.3:12498809-12498828 MS.gene59958:CDS 45.0%
GCTACCACTACCAACATTGA+TGG + chr8.3:12500250-12500269 None:intergenic 45.0%
GGGTTGCTGAAAGTTGCAAT+TGG + chr8.3:12500141-12500160 None:intergenic 45.0%
GTGCAAATGATCCATGGAAC+AGG + chr8.3:12498793-12498812 None:intergenic 45.0%
GTTGTGACTTTCACACCAGA+GGG + chr8.3:12499673-12499692 None:intergenic 45.0%
TAAGTAACAGGTCCAGTAGC+AGG + chr8.3:12499369-12499388 None:intergenic 45.0%
TGTTGTGACTTTCACACCAG+AGG + chr8.3:12499674-12499693 None:intergenic 45.0%
! AACTTCATTTGTGCCGTTCC+AGG - chr8.3:12499141-12499160 MS.gene59958:CDS 45.0%
! GGTAGCTGGACTGTTTGTTA+AGG + chr8.3:12499094-12499113 None:intergenic 45.0%
! GTTGGTGAAGCAGTAGTAGA+TGG + chr8.3:12499333-12499352 None:intergenic 45.0%
! TGCTGCTGCTTATACAACCT+GGG - chr8.3:12499431-12499450 MS.gene59958:intron 45.0%
!! AGAATGAAGCACCGTTGGTT+GGG + chr8.3:12498856-12498875 None:intergenic 45.0%
!! GCCTACAGTGAAGGTTTTGT+TGG + chr8.3:12498894-12498913 None:intergenic 45.0%
!! GGTATAGAATGAAGCACCGT+TGG + chr8.3:12498861-12498880 None:intergenic 45.0%
!! TAGAATGAAGCACCGTTGGT+TGG + chr8.3:12498857-12498876 None:intergenic 45.0%
AAGGAGAAGGTGATGTGGCA+GGG + chr8.3:12500278-12500297 None:intergenic 50.0%
AGATGGAATGGTCCAGCCAA+TGG + chr8.3:12499406-12499425 None:intergenic 50.0%
AGCAGCACCGTTAGATGGAA+TGG + chr8.3:12499418-12499437 None:intergenic 50.0%
CTTGGAGTTGGAGCAGAAAC+AGG + chr8.3:12499222-12499241 None:intergenic 50.0%
GAAGCAGTAGTAGATGGTGC+TGG + chr8.3:12499327-12499346 None:intergenic 50.0%
GAGGACGGTGACAAATAACC+CGG + chr8.3:12500454-12500473 None:intergenic 50.0%
GGCTGGACCATTCCATCTAA+CGG - chr8.3:12499408-12499427 MS.gene59958:intron 50.0%
GGTTGGACTATCCCAACCAA+CGG - chr8.3:12498842-12498861 MS.gene59958:CDS 50.0%
GTTCCAGGACATTGTTCAGC+TGG - chr8.3:12499156-12499175 MS.gene59958:CDS 50.0%
GTTGCTGCAAACCTGTTCCA+TGG - chr8.3:12498779-12498798 MS.gene59958:CDS 50.0%
TAAGCAGCAGCACCGTTAGA+TGG + chr8.3:12499423-12499442 None:intergenic 50.0%
TCACCGACGGTGTAAGTAAC+AGG + chr8.3:12499381-12499400 None:intergenic 50.0%
TCCAAGTGCTTCTCCACCTA+AGG - chr8.3:12499236-12499255 MS.gene59958:intron 50.0%
TCGTTCAATGTCACGGTAGC+TGG + chr8.3:12499108-12499127 None:intergenic 50.0%
TGCAGCTGCAATTGAGAGGA+CGG + chr8.3:12500469-12500488 None:intergenic 50.0%
TGTCCAGCTGAACAATGTCC+TGG + chr8.3:12499162-12499181 None:intergenic 50.0%
TTCACTGTAGGCGACACTCT+CGG - chr8.3:12498902-12498921 MS.gene59958:CDS 50.0%
! AGCAGTAGTAGATGGTGCTG+GGG + chr8.3:12499325-12499344 None:intergenic 50.0%
! GGACCTGTTACTTACACCGT+CGG - chr8.3:12499375-12499394 MS.gene59958:intron 50.0%
! GTGCTGCTGCTTATACAACC+TGG - chr8.3:12499430-12499449 MS.gene59958:intron 50.0%
!! CATGTGGTTGGTGATACCAC+AGG - chr8.3:12498821-12498840 MS.gene59958:CDS 50.0%
!! TGGTTGGTGATACCACAGGT+TGG - chr8.3:12498825-12498844 MS.gene59958:CDS 50.0%
!!! GGTTTTGTTGGAAGCCCAGT+TGG + chr8.3:12498882-12498901 None:intergenic 50.0%
ACCTGGAGACTGGGAAGAAG+GGG + chr8.3:12500379-12500398 None:intergenic 55.0%
CACCTGGAGACTGGGAAGAA+GGG + chr8.3:12500380-12500399 None:intergenic 55.0%
GAAAGACTTGCGAGAAGCCC+AGG + chr8.3:12499451-12499470 None:intergenic 55.0%
GAAGGAGAAGGTGATGTGGC+AGG + chr8.3:12500279-12500298 None:intergenic 55.0%
GACAGAACCACCTGGAGACT+GGG + chr8.3:12500388-12500407 None:intergenic 55.0%
GGTTGGGATAGTCCAACCTG+TGG + chr8.3:12498840-12498859 None:intergenic 55.0%
GTAGGAGCTGGAGTAGCCTT+AGG + chr8.3:12499255-12499274 None:intergenic 55.0%
GTTACTGGACACTGCTCTGC+AGG - chr8.3:12500213-12500232 MS.gene59958:CDS 55.0%
TACACCGTCGGTGACACCAT+TGG - chr8.3:12499387-12499406 MS.gene59958:intron 55.0%
TCATCGCCTTCTCCTTCCAC+AGG - chr8.3:12500303-12500322 MS.gene59958:intron 55.0%
TGACAGAACCACCTGGAGAC+TGG + chr8.3:12500389-12500408 None:intergenic 55.0%
TGTGGAAGGAGAAGGCGATG+AGG + chr8.3:12500304-12500323 None:intergenic 55.0%
! CCCTGTTCCAGCAAAAGCAC+CGG - chr8.3:12499275-12499294 MS.gene59958:intron 55.0%
! CCGGTGCTTTTGCTGGAACA+GGG + chr8.3:12499278-12499297 None:intergenic 55.0%
! CGGTGCTTTTGCTGGAACAG+GGG + chr8.3:12499277-12499296 None:intergenic 55.0%
! GCTTTTGCTGGAACAGGGGT+AGG + chr8.3:12499273-12499292 None:intergenic 55.0%
!! AAAAGCACCGGCACCAACCA+AGG - chr8.3:12499287-12499306 MS.gene59958:intron 55.0%
!! GAGAGTGTCGCCTACAGTGA+AGG + chr8.3:12498903-12498922 None:intergenic 55.0%
!! GCCTTAGGTGGAGAAGCACT+TGG + chr8.3:12499240-12499259 None:intergenic 55.0%
!! GGTGGAGAAGCACTTGGAGT+TGG + chr8.3:12499234-12499253 None:intergenic 55.0%
AACTCCAAGAGACGCGGCAC+CGG + chr8.3:12500430-12500449 None:intergenic 60.0%
ACAGGTCCAGTAGCAGGAGC+TGG + chr8.3:12499363-12499382 None:intergenic 60.0%
ACCCCTTCTTCCCAGTCTCC+AGG - chr8.3:12500375-12500394 MS.gene59958:CDS 60.0%
CCACCTGGAGACTGGGAAGA+AGG + chr8.3:12500381-12500400 None:intergenic 60.0%
CCTTCTTCCCAGTCTCCAGG+TGG - chr8.3:12500378-12500397 MS.gene59958:CDS 60.0%
GACTGGGAAGAAGGGGTGAC+AGG + chr8.3:12500372-12500391 None:intergenic 60.0%
GGAAGGAGAAGGCGATGAGG+CGG + chr8.3:12500301-12500320 None:intergenic 60.0%
GGCGGAAGGAGAAGGTGATG+TGG + chr8.3:12500283-12500302 None:intergenic 60.0%
GTGGCACCTGTGGAAGGAGA+AGG + chr8.3:12500312-12500331 None:intergenic 60.0%
! GCCGGTGCTTTTGCTGGAAC+AGG + chr8.3:12499279-12499298 None:intergenic 60.0%
! GGAGCTGGAGTAGCCTTAGG+TGG + chr8.3:12499252-12499271 None:intergenic 60.0%
! GTAGTAGATGGTGCTGGGGC+AGG + chr8.3:12499321-12499340 None:intergenic 60.0%
!! CCAGCCAATGGTGTCACCGA+CGG + chr8.3:12499394-12499413 None:intergenic 60.0%
!!! GTTGGTGCCGGTGCTTTTGC+TGG + chr8.3:12499285-12499304 None:intergenic 60.0%
AGACCCCGGTGAGGGAGAAC+CGG + chr8.3:12500349-12500368 None:intergenic 65.0%
AGCCCCCCTCCTGAGAACTC+CGG - chr8.3:12500408-12500427 MS.gene59958:CDS 65.0%
CACCGGAGTTCTCAGGAGGG+GGG + chr8.3:12500413-12500432 None:intergenic 65.0%
CCAGTAGCAGGAGCTGGAGC+AGG + chr8.3:12499357-12499376 None:intergenic 65.0%
CCCGGCAACTCCAAGAGACG+CGG + chr8.3:12500436-12500455 None:intergenic 65.0%
CCGCGTCTCTTGGAGTTGCC+GGG - chr8.3:12500433-12500452 MS.gene59958:CDS 65.0%
CCGTCGGTGACACCATTGGC+TGG - chr8.3:12499391-12499410 MS.gene59958:intron 65.0%
CCTGCTCCAGCTCCTGCTAC+TGG - chr8.3:12499354-12499373 MS.gene59958:intron 65.0%
GAAGGGGTGACAGGAGACCC+CGG + chr8.3:12500363-12500382 None:intergenic 65.0%
GCACCGGAGTTCTCAGGAGG+GGG + chr8.3:12500414-12500433 None:intergenic 65.0%
GCAGGAGCTGGAGCAGGTGT+TGG + chr8.3:12499351-12499370 None:intergenic 65.0%
GCAGGTGTAGCGGCCTTGGT+TGG + chr8.3:12499303-12499322 None:intergenic 65.0%
GCCGCGTCTCTTGGAGTTGC+CGG - chr8.3:12500432-12500451 MS.gene59958:CDS 65.0%
GCTGGAACAGGGGTAGGAGC+TGG + chr8.3:12499267-12499286 None:intergenic 65.0%
GGAGAAGGCGATGAGGCGGA+AGG + chr8.3:12500297-12500316 None:intergenic 65.0%
GGCGATGAGGCGGAAGGAGA+AGG + chr8.3:12500291-12500310 None:intergenic 65.0%
GTGACAGGAGACCCCGGTGA+GGG + chr8.3:12500357-12500376 None:intergenic 65.0%
! CGGCACCGGAGTTCTCAGGA+GGG + chr8.3:12500416-12500435 None:intergenic 65.0%
! GGCACCGGAGTTCTCAGGAG+GGG + chr8.3:12500415-12500434 None:intergenic 65.0%
! GTAGCGGCCTTGGTTGGTGC+CGG + chr8.3:12499297-12499316 None:intergenic 65.0%
! TGGTGCTGGGGCAGGTGTAG+CGG + chr8.3:12499313-12499332 None:intergenic 65.0%
!! AACTCCGGTGCCGCGTCTCT+TGG - chr8.3:12500423-12500442 MS.gene59958:CDS 65.0%
CCTCCGGTTCTCCCTCACCG+GGG - chr8.3:12500343-12500362 MS.gene59958:CDS 70.0%
CGCCTCCGGTTCTCCCTCAC+CGG - chr8.3:12500341-12500360 MS.gene59958:CDS 70.0%
CGGTGAGGGAGAACCGGAGG+CGG + chr8.3:12500343-12500362 None:intergenic 70.0%
GAGGGAGAACCGGAGGCGGA+AGG + chr8.3:12500339-12500358 None:intergenic 70.0%
GCCTCCGGTTCTCCCTCACC+GGG - chr8.3:12500342-12500361 MS.gene59958:CDS 70.0%
GGAAGGCGGAGTGGCACCTG+TGG + chr8.3:12500322-12500341 None:intergenic 70.0%
GGAGAACCGGAGGCGGAAGG+CGG + chr8.3:12500336-12500355 None:intergenic 70.0%
GGCGGAGTGGCACCTGTGGA+AGG + chr8.3:12500318-12500337 None:intergenic 70.0%
GGGGGGCTGACAGAACCACC+TGG + chr8.3:12500396-12500415 None:intergenic 70.0%
GGTGACAGGAGACCCCGGTG+AGG + chr8.3:12500358-12500377 None:intergenic 70.0%
TGGGGCAGGTGTAGCGGCCT+TGG + chr8.3:12499307-12499326 None:intergenic 70.0%
! GACGCGGCACCGGAGTTCTC+AGG + chr8.3:12500420-12500439 None:intergenic 70.0%
! GCGGCACCGGAGTTCTCAGG+AGG + chr8.3:12500417-12500436 None:intergenic 70.0%
ACCGGAGGCGGAAGGCGGAG+TGG + chr8.3:12500331-12500350 None:intergenic 75.0%
CCCCGGTGAGGGAGAACCGG+AGG + chr8.3:12500346-12500365 None:intergenic 75.0%
GCCACTCCGCCTTCCGCCTC+CGG - chr8.3:12500327-12500346 MS.gene59958:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 12498737 12500500 12498737 ID=MS.gene59958
chr8.3 mRNA 12498737 12500500 12498737 ID=MS.gene59958.t1;Parent=MS.gene59958
chr8.3 exon 12500314 12500500 12500314 ID=MS.gene59958.t1.exon1;Parent=MS.gene59958.t1
chr8.3 CDS 12500314 12500500 12500314 ID=cds.MS.gene59958.t1;Parent=MS.gene59958.t1
chr8.3 exon 12499748 12500233 12499748 ID=MS.gene59958.t1.exon2;Parent=MS.gene59958.t1
chr8.3 CDS 12499748 12500233 12499748 ID=cds.MS.gene59958.t1;Parent=MS.gene59958.t1
chr8.3 exon 12498737 12499176 12498737 ID=MS.gene59958.t1.exon3;Parent=MS.gene59958.t1
chr8.3 CDS 12498737 12499176 12498737 ID=cds.MS.gene59958.t1;Parent=MS.gene59958.t1
Gene Sequence

>MS.gene59958

ATGGCAAAGAACCTTAATGTTCTTGTTTTTGTTCTACTTGCAGTTGCTGCAAACCTGTTCCATGGATCATTTGCACAAACAAGACATGTGGTTGGTGATACCACAGGTTGGACTATCCCAACCAACGGTGCTTCATTCTATACCAACTGGGCTTCCAACAAAACCTTCACTGTAGGCGACACTCTCGGTAAGAAACAATATATTTCAGCCCTTGTCATTTTGATTCGTTTATATGAGAATTGTTTTAACGTAAACATGATTTTGCAGTGTTCAATTATGCAAGTGGACAACATGATGTTGCTAAGGTAACAAAAACAGCTTACGATAGTTGCAATGGAGCAAACACACTTTTCACCTTAACAAACAGTCCAGCTACCGTGACATTGAACGAAACAGGACAACAAAACTTCATTTGTGCCGTTCCAGGACATTGTTCAGCTGGACAAAAACTTTCAATTAACGTTGTTAAAGCTTCTACTTCACCTGTTTCTGCTCCAACTCCAAGTGCTTCTCCACCTAAGGCTACTCCAGCTCCTACCCCTGTTCCAGCAAAAGCACCGGCACCAACCAAGGCCGCTACACCTGCCCCAGCACCATCTACTACTGCTTCACCAACACCTGCTCCAGCTCCTGCTACTGGACCTGTTACTTACACCGTCGGTGACACCATTGGCTGGACCATTCCATCTAACGGTGCTGCTGCTTATACAACCTGGGCTTCTCGCAAGTCTTTCAAAGTTGGAGACATCCTTGGTAAGTCATGGTTATTGAGAAATTTATTTATGATTAAATACTAGTATTTAATTTTAAGATGAAAAAAATTAGAGATCTTGTACATGTGATCAATGCAGTGTTAGATTTGATTAACCGGGAAATGGATCATCATACGGTGATTTTCACACAAGAGGCTAATGTGCTATAGATTACACATTACCCTCTGGTGTGAAAGTCACAACACATGAGAGGACCCCATTCTTGATTAATAGGTATATCAATTGTCACATCTTATTAATTTATAATTTTACTAACCACTAATTTGAATATGATTAATTATAATTTTAAGTGTTCAACTTCAAATTTACATTATGATTAACAAAATTCAATACATGGTTAATGAGATATCCGATCTCTTGATTAATATATACTCATCAACTATTTGCTTTAAAAGTGTGTGTGAAATGCATTAACCAGGTTGTTGAATTTAGGTATCTAAAATTGTTGTGGAAATATAGTCACTTTTTTTAAATAACTTGTTTAAAAAAAATATGATTTAGATGTATAATTTTGTGAGGCAAATTATTGATCAACTTGTACTATGATGCAGTTTTCAATTTCCAATTGAATGCACACAACGTGGAAGAAGTGACAAAGGAAAAGTATGATTCATGCAACTCAGCTTCTCCAATTGCAACTTTCAGCAACCCACCAGTGAGAGTAACACTTAACAAAACTGGTACTCATTACTATATCTGTGGTGTTACTGGACACTGCTCTGCAGGCCAAAAGCTTTCCATCAATGTTGGTAGTGGTAGCAGCAGCCCTGCCACATCACCTTCTCCTTCCGCCTCATCGCCTTCTCCTTCCACAGGTGCCACTCCGCCTTCCGCCTCCGGTTCTCCCTCACCGGGGTCTCCTGTCACCCCTTCTTCCCAGTCTCCAGGTGGTTCTGTCAGCCCCCCTCCTGAGAACTCCGGTGCCGCGTCTCTTGGAGTTGCCGGGTTATTTGTCACCGTCCTCTCAATTGCAGCTGCATTTTTCTGTTAG
Protein sequence

>MS.gene59958.t1

MAKNLNVLVFVLLAVAANLFHGSFAQTRHVVGDTTGWTIPTNGASFYTNWASNKTFTVGDTLVFNYASGQHDVAKVTKTAYDSCNGANTLFTLTNSPATVTLNETGQQNFICAVPGHCSAGQKLSINVVKASTSPVSAPTPSASPPKATPAPTPVPAKAPAPTKAATPAPAPSTTASPTPAPAPATGPVTYTVGDTIGWTIPSNGAAAYTTWASRKSFKVGDILVFNFQLNAHNVEEVTKEKYDSCNSASPIATFSNPPVRVTLNKTGTHYYICGVTGHCSAGQKLSINVGSGSSSPATSPSPSASSPSPSTGATPPSASGSPSPGSPVTPSSQSPGGSVSPPPENSGAASLGVAGLFVTVLSIAAAFFC