AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60544

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60544.t1 MTR_1g092540 97.822 597 13 0 1 597 1 597 0.0 1212
MS.gene60544.t1 MTR_3g091180 65.174 603 191 6 1 596 1 591 0.0 749
MS.gene60544.t1 MTR_1g061540 39.353 587 260 16 5 571 9 519 8.80e-106 329
MS.gene60544.t1 MTR_1g073890 35.839 572 302 10 16 577 36 552 8.73e-102 320
MS.gene60544.t1 MTR_7g103490 37.931 580 297 10 1 569 5 532 6.60e-101 317
MS.gene60544.t1 MTR_0318s0020 50.000 226 103 6 370 592 351 569 1.35e-61 214
MS.gene60544.t1 MTR_0318s0020 36.364 275 148 5 12 259 114 388 1.13e-45 170
MS.gene60544.t1 MTR_1g044135 58.929 168 63 3 414 578 419 583 1.04e-55 199
MS.gene60544.t1 MTR_1g044135 33.740 246 144 2 1 229 1 244 1.28e-40 156
MS.gene60544.t1 MTR_6g461910 58.642 162 64 3 422 581 342 502 5.80e-52 186
MS.gene60544.t1 MTR_6g461910 39.924 263 141 4 19 279 29 276 5.61e-49 178
MS.gene60544.t1 MTR_4g021260 31.390 223 145 1 15 229 8 230 2.01e-31 128
MS.gene60544.t1 MTR_4g021260 32.661 248 133 9 341 570 242 473 2.69e-30 124
MS.gene60544.t1 MTR_7g063040 29.665 209 142 1 26 229 25 233 7.21e-26 111
MS.gene60544.t1 MTR_7g063040 36.538 156 90 2 423 570 325 479 1.36e-23 104
MS.gene60544.t1 MTR_1g096970 31.707 164 98 2 427 580 263 422 3.09e-17 85.5
MS.gene60544.t1 MTR_1g096970 26.512 215 138 4 26 229 51 256 3.32e-15 79.0
MS.gene60544.t1 MTR_3g114010 32.237 152 89 3 433 574 341 488 5.04e-14 75.5
MS.gene60544.t1 MTR_3g114010 25.837 209 147 2 26 229 122 327 4.53e-13 72.4
MS.gene60544.t1 MTR_1g025340 31.373 153 90 3 433 574 346 494 8.76e-14 74.7
MS.gene60544.t1 MTR_1g025340 25.907 193 135 3 23 210 124 313 8.23e-13 71.6
MS.gene60544.t1 MTR_3g097240 23.039 204 154 2 26 229 141 341 1.22e-12 70.5
MS.gene60544.t1 MTR_3g097240 23.039 204 154 2 26 229 141 341 1.29e-12 70.5
MS.gene60544.t1 MTR_3g097240 23.039 204 154 2 26 229 141 341 1.63e-12 70.9
MS.gene60544.t1 MTR_3g097240 30.147 136 82 3 433 559 357 488 2.67e-11 67.0
MS.gene60544.t1 MTR_8g095080 31.618 136 80 4 433 559 371 502 6.85e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60544.t1 AT5G04550 53.177 598 249 11 1 582 1 583 0.0 557
MS.gene60544.t1 AT5G04550 53.177 598 249 11 1 582 1 583 0.0 557
MS.gene60544.t1 AT5G51670 58.861 158 59 2 428 581 308 463 5.94e-54 191
MS.gene60544.t1 AT5G51670 37.209 215 123 1 26 228 37 251 3.52e-42 159
MS.gene60544.t1 AT5G51670 58.861 158 59 2 428 581 308 463 5.94e-54 191
MS.gene60544.t1 AT5G51670 37.209 215 123 1 26 228 37 251 3.52e-42 159
MS.gene60544.t1 AT3G23160 52.071 169 79 2 419 586 345 512 1.23e-50 183
MS.gene60544.t1 AT3G23160 39.130 207 125 1 23 229 40 245 1.19e-42 161
MS.gene60544.t1 AT5G08660 34.028 144 82 3 435 569 372 511 9.58e-16 81.3
MS.gene60544.t1 AT5G08660 34.028 144 82 3 435 569 372 511 9.58e-16 81.3
MS.gene60544.t1 AT5G08660 34.028 144 82 3 435 569 372 511 9.58e-16 81.3
MS.gene60544.t1 AT1G34320 32.484 157 89 4 430 574 373 524 1.96e-15 80.1
MS.gene60544.t1 AT1G34320 25.000 212 147 3 26 229 155 362 4.86e-15 79.0
MS.gene60544.t1 AT1G34320 32.484 157 89 4 430 574 373 524 1.96e-15 80.1
MS.gene60544.t1 AT1G34320 25.000 212 147 3 26 229 155 362 4.86e-15 79.0
MS.gene60544.t1 AT1G30755 29.814 161 96 3 433 580 353 509 1.15e-12 71.2
MS.gene60544.t1 AT1G30755 26.027 219 148 4 19 229 127 339 8.63e-11 65.5
MS.gene60544.t1 AT1G30755 29.814 161 96 3 433 580 353 509 1.15e-12 71.2
MS.gene60544.t1 AT1G30755 26.027 219 148 4 19 229 127 339 8.63e-11 65.5
MS.gene60544.t1 AT1G30755 29.814 161 96 3 433 580 353 509 1.15e-12 71.2
MS.gene60544.t1 AT1G30755 26.027 219 148 4 19 229 127 339 8.63e-11 65.5
MS.gene60544.t1 AT5G08660 39.535 86 48 1 435 520 372 453 6.42e-11 65.5
MS.gene60544.t1 AT1G30755 26.027 219 148 4 19 229 127 339 8.96e-11 64.7

Find 113 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAATCTGGTAACATTATTT+TGG 0.078697 1.2:-68734947 None:intergenic
ACGAAGTCAGGCCCGATTTC+AGG 0.187337 1.2:+68735062 MS.gene60544:CDS
TTCTCAAAATTCTTCAGTCT+TGG 0.290054 1.2:-68734459 None:intergenic
TCAAGAGATGCTTGAGAGTA+TGG 0.298295 1.2:+68734389 MS.gene60544:CDS
CCGCGAATGTGATCAGAATT+TGG 0.308969 1.2:-68734879 None:intergenic
GTTTCCAAGGGAATATCATT+AGG 0.322766 1.2:-68735317 None:intergenic
CCTCTATACTTTCCTGAAAT+CGG 0.328203 1.2:-68735074 None:intergenic
AATCATGGTTTCGAAATCTA+TGG 0.347401 1.2:+68734157 MS.gene60544:CDS
GTACAATGAGTTACATTTCC+AGG 0.358503 1.2:-68735377 None:intergenic
AGTCTAATTCTTTGTGGAAC+AGG 0.376065 1.2:+68734736 MS.gene60544:CDS
TCTCAAAATTCTTCAGTCTT+GGG 0.381346 1.2:-68734458 None:intergenic
GGCAATAGTGTAGTAGATAA+TGG 0.382722 1.2:+68735896 MS.gene60544:CDS
GCGAGACGAGATTTCAAATT+CGG 0.388087 1.2:+68734311 MS.gene60544:CDS
AATGTTACCAGATTTGCTTC+AGG 0.389947 1.2:+68734954 MS.gene60544:CDS
ACTTACAGTCTAATTCTTTG+TGG 0.399040 1.2:+68734730 MS.gene60544:CDS
TTCTGATACTCGATCAAATT+CGG 0.401763 1.2:-68734675 None:intergenic
AAGGGGTCCTGAAGCAAATC+TGG 0.403486 1.2:-68734961 None:intergenic
ACAGCCGCAGCCATGGAATA+TGG 0.403752 1.2:-68735605 None:intergenic
TTCTTGATTCGCAAAGTAAA+GGG 0.409376 1.2:-68735783 None:intergenic
CCTGTCCCGGATAATGTAAC+AGG 0.409704 1.2:+68735140 MS.gene60544:CDS
TTTCCAAGGGAATATCATTA+GGG 0.411335 1.2:-68735316 None:intergenic
CTCAGAGTTATTGCAATCTT+CGG 0.412776 1.2:+68734914 MS.gene60544:CDS
TTCGCGGACCAACGTGTTGT+TGG 0.427189 1.2:+68735754 MS.gene60544:CDS
AATTCTGAGAAAGCAGTGAT+TGG 0.430163 1.2:+68734204 MS.gene60544:CDS
CGGCGAGCATTTCCATCTCT+TGG 0.439910 1.2:-68734602 None:intergenic
GGCGACTCTTCGACGAAGTC+AGG 0.440304 1.2:+68735050 MS.gene60544:CDS
TTTCTTGATTCGCAAAGTAA+AGG 0.440538 1.2:-68735784 None:intergenic
CAATAACATTTGCGTAGTGC+AGG 0.451579 1.2:-68735469 None:intergenic
TGATCTGATTTAGCAGAAGA+AGG 0.453628 1.2:-68734987 None:intergenic
CTCTATACTTTCCTGAAATC+GGG 0.454304 1.2:-68735073 None:intergenic
GCAGTCTTTGAGTGATAAAC+AGG 0.455192 1.2:+68734278 MS.gene60544:CDS
CGCAAGCCTAGCCACAGATT+CGG 0.460018 1.2:-68734421 None:intergenic
TGATCACATTCGCGGCAGTC+AGG 0.460605 1.2:+68734887 MS.gene60544:CDS
AATACAGGTATCTTAGAGAT+TGG 0.462810 1.2:+68735875 MS.gene60544:CDS
AGCAGCTGAGCTTTGTTTCG+CGG 0.466399 1.2:+68735738 MS.gene60544:CDS
TCAGCCTCTCCACACTTGAT+TGG 0.470012 1.2:+68735509 MS.gene60544:CDS
GCAGGTCGCGTGGAAGAAGC+TGG 0.472548 1.2:+68734698 MS.gene60544:CDS
CCTGTTACATTATCCGGGAC+AGG 0.480680 1.2:-68735140 None:intergenic
AGATTTGCTTCAGGACCCCT+TGG 0.481762 1.2:+68734963 MS.gene60544:CDS
TGAAATCTCGTCTCGCAACT+TGG 0.482654 1.2:-68734304 None:intergenic
GATTTAGACCTTCATACTCC+TGG 0.485555 1.2:+68735359 MS.gene60544:CDS
TATGTGAAGGAGCTCAATAC+AGG 0.487172 1.2:+68735860 MS.gene60544:CDS
ATTGAAGAGGTAATAAATGT+TGG 0.500472 1.2:+68734834 MS.gene60544:CDS
CCAAATTCTGATCACATTCG+CGG 0.501108 1.2:+68734879 MS.gene60544:CDS
GGCTAAAGAAGCGGCACCGA+GGG 0.505212 1.2:-68735447 None:intergenic
CTGCCACACATTGACAAGCT+TGG 0.505389 1.2:-68734259 None:intergenic
GGCGTGTAAGAGCTTCTCTC+AGG 0.517048 1.2:+68735570 MS.gene60544:CDS
ACTAGCATTCTTCACTCGGT+TGG 0.524138 1.2:+68735029 MS.gene60544:CDS
ATAATCACCGAGCTTCTTGT+CGG 0.528578 1.2:+68735818 MS.gene60544:CDS
ACTTGATTGGTCTCGATGCA+AGG 0.529764 1.2:+68735522 MS.gene60544:CDS
TTCGATATGTTTATTACCCG+TGG 0.530555 1.2:+68734486 MS.gene60544:CDS
CTTAACATGTTGTGAGCAAG+TGG 0.530855 1.2:-68735692 None:intergenic
TGCAAGCTTGTACCAAGAGA+TGG 0.531229 1.2:+68734590 MS.gene60544:CDS
CCGATTTCAGGAAAGTATAG+AGG 0.533260 1.2:+68735074 MS.gene60544:CDS
TTACATTTCCAGGAGTATGA+AGG 0.535650 1.2:-68735367 None:intergenic
ACGCAAGTCTAGCAGAAGAA+TGG 0.537556 1.2:+68735633 MS.gene60544:CDS
AAACCCTAATGATATTCCCT+TGG 0.537691 1.2:+68735313 MS.gene60544:CDS
CTTTATTTACAATACTCAGT+AGG 0.546289 1.2:+68734793 MS.gene60544:CDS
TAAAACTAGCATTCTTCACT+CGG 0.549237 1.2:+68735025 MS.gene60544:CDS
TTGCTCACAACATGTTAAGA+TGG 0.550366 1.2:+68735696 MS.gene60544:CDS
GCATACTCTGAAGAGAATGA+AGG 0.558376 1.2:+68734632 MS.gene60544:CDS
TTTGTGATTCTGAGTTTCCA+AGG 0.559767 1.2:-68735330 None:intergenic
AGGTTTACAGTGAGTCGGAC+GGG 0.562547 1.2:+68734652 MS.gene60544:CDS
TCCCACTCGTGGTTGTTGAG+TGG 0.562845 1.2:-68735196 None:intergenic
AAGCGATGGTAGCTGAATCA+TGG 0.567030 1.2:+68734142 None:intergenic
CTTGATTGGTCTCGATGCAA+GGG 0.571162 1.2:+68735523 MS.gene60544:CDS
TTTGATCGAGTATCAGAAGC+AGG 0.574774 1.2:+68734680 MS.gene60544:CDS
TTGTGATTCTGAGTTTCCAA+GGG 0.576032 1.2:-68735329 None:intergenic
TAAGAGCTTCTCTCAGGACC+AGG 0.577584 1.2:+68735576 MS.gene60544:CDS
CCCATACGAATCAAAACCAC+GGG 0.579429 1.2:-68734502 None:intergenic
ATGTGGCCGAATCTGTGGCT+AGG 0.583300 1.2:+68734415 MS.gene60544:CDS
GCACCGAGGGATTCAGATGG+AGG 0.584223 1.2:-68735434 None:intergenic
AGTATCAGAAGCAGGTCGCG+TGG 0.584490 1.2:+68734688 MS.gene60544:CDS
CAGGCTTAAGCCATATTCCA+TGG 0.584717 1.2:+68735595 MS.gene60544:CDS
CATAGTCGAGCAACTCAAGT+AGG 0.589346 1.2:+68735236 MS.gene60544:CDS
CTTCAGAGTATGCTCAAGAT+CGG 0.590154 1.2:-68734622 None:intergenic
AAGCCTCCATCTGAATCCCT+CGG 0.594289 1.2:+68735431 MS.gene60544:CDS
GTCGTAAACAGCCGCAGCCA+TGG 0.595146 1.2:-68735612 None:intergenic
TTGTCGGTCTGAATTATGTG+TGG 0.595673 1.2:+68735834 MS.gene60544:CDS
ATCTATACAACATGCTGCCG+AGG 0.599453 1.2:+68735549 MS.gene60544:CDS
CATGGAATATGGCTTAAGCC+TGG 0.599747 1.2:-68735594 None:intergenic
GACGAGATTTCAAATTCGGT+CGG 0.601247 1.2:+68734315 MS.gene60544:CDS
GGCACATGTGGCCGAATCTG+TGG 0.603173 1.2:+68734410 MS.gene60544:CDS
TCTATGGAAGACTCAACGTA+AGG 0.603403 1.2:+68734173 MS.gene60544:CDS
TTGCAAAGAGAACTGAATGT+CGG 0.605976 1.2:-68735404 None:intergenic
GCGGCAATACAGCCTTTGAA+AGG 0.610443 1.2:-68735260 None:intergenic
ATTCAGACCGACAAGAAGCT+CGG 0.611368 1.2:-68735825 None:intergenic
AATAACATTTGCGTAGTGCA+GGG 0.614388 1.2:-68735468 None:intergenic
GGCCACTCAACAACCACGAG+TGG 0.614960 1.2:+68735194 MS.gene60544:CDS
CTCAACAACCACGAGTGGGA+AGG 0.617609 1.2:+68735199 MS.gene60544:CDS
TAAGCCATATTCCATGGCTG+CGG 0.620281 1.2:+68735601 MS.gene60544:CDS
AAGGTTTACAGTGAGTCGGA+CGG 0.625325 1.2:+68734651 MS.gene60544:CDS
ACCCATACGAATCAAAACCA+CGG 0.629560 1.2:-68734503 None:intergenic
AATGAAGGTTTACAGTGAGT+CGG 0.629961 1.2:+68734647 MS.gene60544:CDS
AGAGAAGCTCTTACACGCCT+CGG 0.633061 1.2:-68735566 None:intergenic
GCGGCACCGAGGGATTCAGA+TGG 0.634579 1.2:-68735437 None:intergenic
ACGGGTAATAAACATATCGA+AGG 0.648664 1.2:-68734484 None:intergenic
TTTAGCAGAAGAAGGACCAA+GGG 0.649099 1.2:-68734979 None:intergenic
GCCACTCAACAACCACGAGT+GGG 0.651051 1.2:+68735195 MS.gene60544:CDS
GTAGTGCAGGGCTAAAGAAG+CGG 0.653446 1.2:-68735456 None:intergenic
GCTTGAGAGTATGGCACATG+TGG 0.659391 1.2:+68734398 MS.gene60544:CDS
ACTCAAGTAGGACCTTTCAA+AGG 0.660547 1.2:+68735248 MS.gene60544:CDS
GATGACTGAGGAACTATCTG+CGG 0.666924 1.2:-68735279 None:intergenic
ATTTAGCAGAAGAAGGACCA+AGG 0.666925 1.2:-68734980 None:intergenic
GCATCGAGACCAATCAAGTG+TGG 0.668078 1.2:-68735518 None:intergenic
GAGACCAATCAAGTGTGGAG+AGG 0.678449 1.2:-68735513 None:intergenic
GGGCTAAAGAAGCGGCACCG+AGG 0.679045 1.2:-68735448 None:intergenic
TGTCCAAGCTTGTCAATGTG+TGG 0.689258 1.2:+68734256 MS.gene60544:CDS
GGTCGCGTGGAAGAAGCTGG+AGG 0.699243 1.2:+68734701 MS.gene60544:CDS
TTATGTGTGGAGATATGTGA+AGG 0.710243 1.2:+68735847 MS.gene60544:CDS
GGTTTGTACCAACAACACGT+TGG 0.715857 1.2:-68735762 None:intergenic
TTAGCAGAAGAAGGACCAAG+GGG 0.723066 1.2:-68734978 None:intergenic
TGAGTGACAGTCGATGACTG+AGG 0.725297 1.2:-68735291 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAAGAAAATGGATAAAA+AGG + chr1.2:68734536-68734555 MS.gene60544:CDS 15.0%
!! CAATGACAAAAATATTAGAA+TGG + chr1.2:68735663-68735682 MS.gene60544:CDS 20.0%
!!! AAATCTGGTAACATTATTTT+GGG - chr1.2:68734949-68734968 None:intergenic 20.0%
!!! AAGTTTTGGAAGTTTAATTA+TGG + chr1.2:68735173-68735192 MS.gene60544:CDS 20.0%
! AAATGGATAAAAAGGTCAAA+AGG + chr1.2:68734544-68734563 MS.gene60544:CDS 25.0%
! ATTGAAGAGGTAATAAATGT+TGG + chr1.2:68734834-68734853 MS.gene60544:CDS 25.0%
!! CAAATCTGGTAACATTATTT+TGG - chr1.2:68734950-68734969 None:intergenic 25.0%
!! CTTTATTTACAATACTCAGT+AGG + chr1.2:68734793-68734812 MS.gene60544:CDS 25.0%
!! TTTTTACCTGTTACATTATC+CGG - chr1.2:68735149-68735168 None:intergenic 25.0%
!!! GGTAATGTCTTTTAAGAAAA+TGG + chr1.2:68734527-68734546 MS.gene60544:CDS 25.0%
AATACAGGTATCTTAGAGAT+TGG + chr1.2:68735875-68735894 MS.gene60544:CDS 30.0%
AATCATGGTTTCGAAATCTA+TGG + chr1.2:68734157-68734176 MS.gene60544:CDS 30.0%
ACTTACAGTCTAATTCTTTG+TGG + chr1.2:68734730-68734749 MS.gene60544:CDS 30.0%
TAAAACTAGCATTCTTCACT+CGG + chr1.2:68735025-68735044 MS.gene60544:CDS 30.0%
TCTCAAAATTCTTCAGTCTT+GGG - chr1.2:68734461-68734480 None:intergenic 30.0%
TTCTCAAAATTCTTCAGTCT+TGG - chr1.2:68734462-68734481 None:intergenic 30.0%
TTCTGATACTCGATCAAATT+CGG - chr1.2:68734678-68734697 None:intergenic 30.0%
TTTCCAAGGGAATATCATTA+GGG - chr1.2:68735319-68735338 None:intergenic 30.0%
! AGTAGGATCAACAAAGTTTT+TGG + chr1.2:68734810-68734829 MS.gene60544:CDS 30.0%
! TTCTTGATTCGCAAAGTAAA+GGG - chr1.2:68735786-68735805 None:intergenic 30.0%
! TTTCTTGATTCGCAAAGTAA+AGG - chr1.2:68735787-68735806 None:intergenic 30.0%
! TTTTACCTGTTACATTATCC+GGG - chr1.2:68735148-68735167 None:intergenic 30.0%
!! AAGACTGAAGAATTTTGAGA+AGG + chr1.2:68734461-68734480 MS.gene60544:CDS 30.0%
!!! CAAAGTTTTTGGAATTGAAG+AGG + chr1.2:68734821-68734840 MS.gene60544:CDS 30.0%
!!! TAACATTATTTTGGGATGGA+TGG - chr1.2:68734941-68734960 None:intergenic 30.0%
!!! TAGAGGCATCAATTTTTTCT+CGG + chr1.2:68735091-68735110 MS.gene60544:CDS 30.0%
AAACCCTAATGATATTCCCT+TGG + chr1.2:68735313-68735332 MS.gene60544:CDS 35.0%
AAGTTCAAAAAAACCTGTCC+CGG + chr1.2:68735127-68735146 MS.gene60544:CDS 35.0%
AATAACATTTGCGTAGTGCA+GGG - chr1.2:68735471-68735490 None:intergenic 35.0%
AATGAAGGTTTACAGTGAGT+CGG + chr1.2:68734647-68734666 MS.gene60544:CDS 35.0%
ACGGGTAATAAACATATCGA+AGG - chr1.2:68734487-68734506 None:intergenic 35.0%
AGTCTAATTCTTTGTGGAAC+AGG + chr1.2:68734736-68734755 MS.gene60544:CDS 35.0%
CCTCTATACTTTCCTGAAAT+CGG - chr1.2:68735077-68735096 None:intergenic 35.0%
CTCAGAGTTATTGCAATCTT+CGG + chr1.2:68734914-68734933 MS.gene60544:CDS 35.0%
CTCTATACTTTCCTGAAATC+GGG - chr1.2:68735076-68735095 None:intergenic 35.0%
GGATAAAAAGGTCAAAAGGA+TGG + chr1.2:68734548-68734567 MS.gene60544:CDS 35.0%
GGCAATAGTGTAGTAGATAA+TGG + chr1.2:68735896-68735915 MS.gene60544:CDS 35.0%
GTACAATGAGTTACATTTCC+AGG - chr1.2:68735380-68735399 None:intergenic 35.0%
GTTTCCAAGGGAATATCATT+AGG - chr1.2:68735320-68735339 None:intergenic 35.0%
TGATCTGATTTAGCAGAAGA+AGG - chr1.2:68734990-68735009 None:intergenic 35.0%
TTATGTGTGGAGATATGTGA+AGG + chr1.2:68735847-68735866 MS.gene60544:CDS 35.0%
TTCGATATGTTTATTACCCG+TGG + chr1.2:68734486-68734505 MS.gene60544:CDS 35.0%
TTGCAAAGAGAACTGAATGT+CGG - chr1.2:68735407-68735426 None:intergenic 35.0%
TTGCTCACAACATGTTAAGA+TGG + chr1.2:68735696-68735715 MS.gene60544:CDS 35.0%
TTGTGATTCTGAGTTTCCAA+GGG - chr1.2:68735332-68735351 None:intergenic 35.0%
TTTGTGATTCTGAGTTTCCA+AGG - chr1.2:68735333-68735352 None:intergenic 35.0%
! AATGTTACCAGATTTGCTTC+AGG + chr1.2:68734954-68734973 MS.gene60544:CDS 35.0%
! AATTCTGAGAAAGCAGTGAT+TGG + chr1.2:68734204-68734223 MS.gene60544:CDS 35.0%
! CAGGTAAAAACAGCAAGTTT+TGG + chr1.2:68735159-68735178 MS.gene60544:CDS 35.0%
! TTACATTTCCAGGAGTATGA+AGG - chr1.2:68735370-68735389 None:intergenic 35.0%
!!! AGAGGCATCAATTTTTTCTC+GGG + chr1.2:68735092-68735111 MS.gene60544:CDS 35.0%
!!! CTGGTAACATTATTTTGGGA+TGG - chr1.2:68734945-68734964 None:intergenic 35.0%
!!! TTTTTTGAACTTCTTCCGAG+AGG - chr1.2:68735119-68735138 None:intergenic 35.0%
ACCCATACGAATCAAAACCA+CGG - chr1.2:68734506-68734525 None:intergenic 40.0%
ACTCAAGTAGGACCTTTCAA+AGG + chr1.2:68735248-68735267 MS.gene60544:CDS 40.0%
ATAATCACCGAGCTTCTTGT+CGG + chr1.2:68735818-68735837 MS.gene60544:CDS 40.0%
ATTTAGCAGAAGAAGGACCA+AGG - chr1.2:68734983-68735002 None:intergenic 40.0%
CAATAACATTTGCGTAGTGC+AGG - chr1.2:68735472-68735491 None:intergenic 40.0%
CCAAATTCTGATCACATTCG+CGG + chr1.2:68734879-68734898 MS.gene60544:CDS 40.0%
CTTAACATGTTGTGAGCAAG+TGG - chr1.2:68735695-68735714 None:intergenic 40.0%
CTTCAGAGTATGCTCAAGAT+CGG - chr1.2:68734625-68734644 None:intergenic 40.0%
GACGAGATTTCAAATTCGGT+CGG + chr1.2:68734315-68734334 MS.gene60544:CDS 40.0%
GATTTAGACCTTCATACTCC+TGG + chr1.2:68735359-68735378 MS.gene60544:CDS 40.0%
GCAGTCTTTGAGTGATAAAC+AGG + chr1.2:68734278-68734297 MS.gene60544:CDS 40.0%
GCGAGACGAGATTTCAAATT+CGG + chr1.2:68734311-68734330 MS.gene60544:CDS 40.0%
TATGTGAAGGAGCTCAATAC+AGG + chr1.2:68735860-68735879 MS.gene60544:CDS 40.0%
TCAAGAGATGCTTGAGAGTA+TGG + chr1.2:68734389-68734408 MS.gene60544:CDS 40.0%
TCTATGGAAGACTCAACGTA+AGG + chr1.2:68734173-68734192 MS.gene60544:CDS 40.0%
TTGTCGGTCTGAATTATGTG+TGG + chr1.2:68735834-68735853 MS.gene60544:CDS 40.0%
TTTAGCAGAAGAAGGACCAA+GGG - chr1.2:68734982-68735001 None:intergenic 40.0%
TTTGATCGAGTATCAGAAGC+AGG + chr1.2:68734680-68734699 MS.gene60544:CDS 40.0%
! CCGATTTCAGGAAAGTATAG+AGG + chr1.2:68735074-68735093 MS.gene60544:CDS 40.0%
! GCATACTCTGAAGAGAATGA+AGG + chr1.2:68734632-68734651 MS.gene60544:CDS 40.0%
!!! AATTTTTTCTCGGGACCTCT+CGG + chr1.2:68735101-68735120 MS.gene60544:CDS 40.0%
!!! TGGTTTTGATTCGTATGGGT+GGG + chr1.2:68734506-68734525 MS.gene60544:CDS 40.0%
AAGGTTTACAGTGAGTCGGA+CGG + chr1.2:68734651-68734670 MS.gene60544:CDS 45.0%
ACGCAAGTCTAGCAGAAGAA+TGG + chr1.2:68735633-68735652 MS.gene60544:CDS 45.0%
ACTAGCATTCTTCACTCGGT+TGG + chr1.2:68735029-68735048 MS.gene60544:CDS 45.0%
ATCTATACAACATGCTGCCG+AGG + chr1.2:68735549-68735568 MS.gene60544:CDS 45.0%
ATTCAGACCGACAAGAAGCT+CGG - chr1.2:68735828-68735847 None:intergenic 45.0%
CAGGCTTAAGCCATATTCCA+TGG + chr1.2:68735595-68735614 MS.gene60544:CDS 45.0%
CATAGTCGAGCAACTCAAGT+AGG + chr1.2:68735236-68735255 MS.gene60544:CDS 45.0%
CATGGAATATGGCTTAAGCC+TGG - chr1.2:68735597-68735616 None:intergenic 45.0%
CCCATACGAATCAAAACCAC+GGG - chr1.2:68734505-68734524 None:intergenic 45.0%
CCGCGAATGTGATCAGAATT+TGG - chr1.2:68734882-68734901 None:intergenic 45.0%
GATGACTGAGGAACTATCTG+CGG - chr1.2:68735282-68735301 None:intergenic 45.0%
GGTTTGTACCAACAACACGT+TGG - chr1.2:68735765-68735784 None:intergenic 45.0%
TAAGCCATATTCCATGGCTG+CGG + chr1.2:68735601-68735620 MS.gene60544:CDS 45.0%
TGAAATCTCGTCTCGCAACT+TGG - chr1.2:68734307-68734326 None:intergenic 45.0%
TGCAAGCTTGTACCAAGAGA+TGG + chr1.2:68734590-68734609 MS.gene60544:CDS 45.0%
TGTCCAAGCTTGTCAATGTG+TGG + chr1.2:68734256-68734275 MS.gene60544:CDS 45.0%
TTAGCAGAAGAAGGACCAAG+GGG - chr1.2:68734981-68735000 None:intergenic 45.0%
!! ACTTGATTGGTCTCGATGCA+AGG + chr1.2:68735522-68735541 MS.gene60544:CDS 45.0%
!! CTTGATTGGTCTCGATGCAA+GGG + chr1.2:68735523-68735542 MS.gene60544:CDS 45.0%
!! GTTATTTTCCTTCCCACTCG+TGG - chr1.2:68735210-68735229 None:intergenic 45.0%
!!! ACCCGTGGTTTTGATTCGTA+TGG + chr1.2:68734501-68734520 MS.gene60544:CDS 45.0%
!!! CCCGTGGTTTTGATTCGTAT+GGG + chr1.2:68734502-68734521 MS.gene60544:CDS 45.0%
!!! GTGGTTTTGATTCGTATGGG+TGG + chr1.2:68734505-68734524 MS.gene60544:CDS 45.0%
AAGCCTCCATCTGAATCCCT+CGG + chr1.2:68735431-68735450 MS.gene60544:CDS 50.0%
AAGGGGTCCTGAAGCAAATC+TGG - chr1.2:68734964-68734983 None:intergenic 50.0%
AGAGAAGCTCTTACACGCCT+CGG - chr1.2:68735569-68735588 None:intergenic 50.0%
AGGTTTACAGTGAGTCGGAC+GGG + chr1.2:68734652-68734671 MS.gene60544:CDS 50.0%
CCTGTCCCGGATAATGTAAC+AGG + chr1.2:68735140-68735159 MS.gene60544:CDS 50.0%
CCTGTTACATTATCCGGGAC+AGG - chr1.2:68735143-68735162 None:intergenic 50.0%
CTGCCACACATTGACAAGCT+TGG - chr1.2:68734262-68734281 None:intergenic 50.0%
GAGACCAATCAAGTGTGGAG+AGG - chr1.2:68735516-68735535 None:intergenic 50.0%
GCATCGAGACCAATCAAGTG+TGG - chr1.2:68735521-68735540 None:intergenic 50.0%
GCGGCAATACAGCCTTTGAA+AGG - chr1.2:68735263-68735282 None:intergenic 50.0%
GTAGTGCAGGGCTAAAGAAG+CGG - chr1.2:68735459-68735478 None:intergenic 50.0%
TAAGAGCTTCTCTCAGGACC+AGG + chr1.2:68735576-68735595 MS.gene60544:CDS 50.0%
TCAGCCTCTCCACACTTGAT+TGG + chr1.2:68735509-68735528 MS.gene60544:CDS 50.0%
TGAGTGACAGTCGATGACTG+AGG - chr1.2:68735294-68735313 None:intergenic 50.0%
! AGATTTGCTTCAGGACCCCT+TGG + chr1.2:68734963-68734982 MS.gene60544:CDS 50.0%
! GCTTGAGAGTATGGCACATG+TGG + chr1.2:68734398-68734417 MS.gene60544:CDS 50.0%
!! AGCAGCTGAGCTTTGTTTCG+CGG + chr1.2:68735738-68735757 MS.gene60544:CDS 50.0%
ACAGCCGCAGCCATGGAATA+TGG - chr1.2:68735608-68735627 None:intergenic 55.0%
ACGAAGTCAGGCCCGATTTC+AGG + chr1.2:68735062-68735081 MS.gene60544:CDS 55.0%
AGTATCAGAAGCAGGTCGCG+TGG + chr1.2:68734688-68734707 MS.gene60544:CDS 55.0%
ATGTGGCCGAATCTGTGGCT+AGG + chr1.2:68734415-68734434 MS.gene60544:CDS 55.0%
CGCAAGCCTAGCCACAGATT+CGG - chr1.2:68734424-68734443 None:intergenic 55.0%
CGGCGAGCATTTCCATCTCT+TGG - chr1.2:68734605-68734624 None:intergenic 55.0%
CTCAACAACCACGAGTGGGA+AGG + chr1.2:68735199-68735218 MS.gene60544:CDS 55.0%
GCCACTCAACAACCACGAGT+GGG + chr1.2:68735195-68735214 MS.gene60544:CDS 55.0%
GGCGTGTAAGAGCTTCTCTC+AGG + chr1.2:68735570-68735589 MS.gene60544:CDS 55.0%
TCCCACTCGTGGTTGTTGAG+TGG - chr1.2:68735199-68735218 None:intergenic 55.0%
TGATCACATTCGCGGCAGTC+AGG + chr1.2:68734887-68734906 MS.gene60544:CDS 55.0%
TTCGCGGACCAACGTGTTGT+TGG + chr1.2:68735754-68735773 MS.gene60544:CDS 55.0%
GCACCGAGGGATTCAGATGG+AGG - chr1.2:68735437-68735456 None:intergenic 60.0%
GGCACATGTGGCCGAATCTG+TGG + chr1.2:68734410-68734429 MS.gene60544:CDS 60.0%
GGCCACTCAACAACCACGAG+TGG + chr1.2:68735194-68735213 MS.gene60544:CDS 60.0%
GGCGACTCTTCGACGAAGTC+AGG + chr1.2:68735050-68735069 MS.gene60544:CDS 60.0%
GTCGTAAACAGCCGCAGCCA+TGG - chr1.2:68735615-68735634 None:intergenic 60.0%
! GGCTAAAGAAGCGGCACCGA+GGG - chr1.2:68735450-68735469 None:intergenic 60.0%
GCAGGTCGCGTGGAAGAAGC+TGG + chr1.2:68734698-68734717 MS.gene60544:CDS 65.0%
GGGCTAAAGAAGCGGCACCG+AGG - chr1.2:68735451-68735470 None:intergenic 65.0%
GGTCGCGTGGAAGAAGCTGG+AGG + chr1.2:68734701-68734720 MS.gene60544:CDS 65.0%
! GCGGCACCGAGGGATTCAGA+TGG - chr1.2:68735440-68735459 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 68734147 68735940 68734147 ID=MS.gene60544
chr1.2 mRNA 68734147 68735940 68734147 ID=MS.gene60544.t1;Parent=MS.gene60544
chr1.2 exon 68734147 68735940 68734147 ID=MS.gene60544.t1.exon1;Parent=MS.gene60544.t1
chr1.2 CDS 68734147 68735940 68734147 ID=cds.MS.gene60544.t1;Parent=MS.gene60544.t1
Gene Sequence

>MS.gene60544

ATGGTAGCTGAATCATGGTTTCGAAATCTATGGAAGACTCAACGTAAGGATGATTCTAATTCTGAGAAAGCAGTGATTGGAGTGTTAGCATTTGAAGCTGCGAAATCGATGTCCAAGCTTGTCAATGTGTGGCAGTCTTTGAGTGATAAACAGGTTGCCAAGTTGCGAGACGAGATTTCAAATTCGGTCGGTATCAAGAAGCTTGTTTCAGATGATGAGAATTTTATCGTACGTTTAATCAGTCAAGAGATGCTTGAGAGTATGGCACATGTGGCCGAATCTGTGGCTAGGCTTGCGAAGAAATGCAGTGACCCAAGACTGAAGAATTTTGAGAAGGCCTTCGATATGTTTATTACCCGTGGTTTTGATTCGTATGGGTGGGTAATGTCTTTTAAGAAAATGGATAAAAAGGTCAAAAGGATGGAAAAGTTTGTATCGATTAATGCAAGCTTGTACCAAGAGATGGAAATGCTCGCCGATCTTGAGCATACTCTGAAGAGAATGAAGGTTTACAGTGAGTCGGACGGGCCGAATTTGATCGAGTATCAGAAGCAGGTCGCGTGGAAGAAGCTGGAGGTGAAGAACTTACAGTCTAATTCTTTGTGGAACAGGACATATGATTACACTGTTCTCTTTTTAGCAAGATCTTTATTTACAATACTCAGTAGGATCAACAAAGTTTTTGGAATTGAAGAGGTAATAAATGTTGGTAAAAGCATGAATCGTAGTGTTCCAAATTCTGATCACATTCGCGGCAGTCAGGCAGTCTCAGAGTTATTGCAATCTTCGGTCCATCCATCCCAAAATAATGTTACCAGATTTGCTTCAGGACCCCTTGGTCCTTCTTCTGCTAAATCAGATCAAAATGTTAGAGCAAATAAAACTAGCATTCTTCACTCGGTTGGCGACTCTTCGACGAAGTCAGGCCCGATTTCAGGAAAGTATAGAGGCATCAATTTTTTCTCGGGACCTCTCGGAAGAAGTTCAAAAAAACCTGTCCCGGATAATGTAACAGGTAAAAACAGCAAGTTTTGGAAGTTTAATTATGGCCACTCAACAACCACGAGTGGGAAGGAAAATAACACTAGACATAGTCGAGCAACTCAAGTAGGACCTTTCAAAGGCTGTATTGCCGCAGATAGTTCCTCAGTCATCGACTGTCACTCAAACCCTAATGATATTCCCTTGGAAACTCAGAATCACAAAGATGCTGATTTAGACCTTCATACTCCTGGAAATGTAACTCATTGTACTAAACCGACATTCAGTTCTCTTTGCAAGTTAAAGCCTCCATCTGAATCCCTCGGTGCCGCTTCTTTAGCCCTGCACTACGCAAATGTTATTGTCGTGATTGAGAAGCTATCAGCCTCTCCACACTTGATTGGTCTCGATGCAAGGGATGATCTATACAACATGCTGCCGAGGCGTGTAAGAGCTTCTCTCAGGACCAGGCTTAAGCCATATTCCATGGCTGCGGCTGTTTACGACGCAAGTCTAGCAGAAGAATGGAGCGAAGCAATGACAAAAATATTAGAATGGTTAGCTCCACTTGCTCACAACATGTTAAGATGGCAATCTGAGAGAAGTTACGAGCAGCTGAGCTTTGTTTCGCGGACCAACGTGTTGTTGGTACAAACCCTTTACTTTGCGAATCAAGAAAAAACAGAAGCAATAATCACCGAGCTTCTTGTCGGTCTGAATTATGTGTGGAGATATGTGAAGGAGCTCAATACAGGTATCTTAGAGATTGGCAATAGTGTAGTAGATAATGGATATTCTCTGCTGAATGCATAA
Protein sequence

>MS.gene60544.t1

MVAESWFRNLWKTQRKDDSNSEKAVIGVLAFEAAKSMSKLVNVWQSLSDKQVAKLRDEISNSVGIKKLVSDDENFIVRLISQEMLESMAHVAESVARLAKKCSDPRLKNFEKAFDMFITRGFDSYGWVMSFKKMDKKVKRMEKFVSINASLYQEMEMLADLEHTLKRMKVYSESDGPNLIEYQKQVAWKKLEVKNLQSNSLWNRTYDYTVLFLARSLFTILSRINKVFGIEEVINVGKSMNRSVPNSDHIRGSQAVSELLQSSVHPSQNNVTRFASGPLGPSSAKSDQNVRANKTSILHSVGDSSTKSGPISGKYRGINFFSGPLGRSSKKPVPDNVTGKNSKFWKFNYGHSTTTSGKENNTRHSRATQVGPFKGCIAADSSSVIDCHSNPNDIPLETQNHKDADLDLHTPGNVTHCTKPTFSSLCKLKPPSESLGAASLALHYANVIVVIEKLSASPHLIGLDARDDLYNMLPRRVRASLRTRLKPYSMAAAVYDASLAEEWSEAMTKILEWLAPLAHNMLRWQSERSYEQLSFVSRTNVLLVQTLYFANQEKTEAIITELLVGLNYVWRYVKELNTGILEIGNSVVDNGYSLLNA