Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61472.t1 | XP_003609267.2 | 97.1 | 140 | 4 | 0 | 5 | 144 | 1 | 140 | 1.50E-83 | 318.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61472.t1 | G7JVA8 | 97.1 | 140 | 4 | 0 | 5 | 144 | 1 | 140 | 1.1e-83 | 318.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene61472 | MS.gene072918 | PPI |
| MS.gene61472 | MS.gene014815 | PPI |
| MS.gene32612 | MS.gene61472 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61472.t1 | MTR_4g113840 | 97.143 | 140 | 4 | 0 | 5 | 144 | 1 | 140 | 4.53e-98 | 278 |
| MS.gene61472.t1 | MTR_2g054750 | 71.429 | 140 | 28 | 1 | 5 | 144 | 1 | 128 | 3.70e-70 | 207 |
| MS.gene61472.t1 | MTR_4g011190 | 48.252 | 143 | 59 | 3 | 10 | 139 | 5 | 145 | 2.92e-40 | 132 |
| MS.gene61472.t1 | MTR_3g107610 | 44.025 | 159 | 57 | 6 | 10 | 139 | 5 | 160 | 6.39e-31 | 109 |
| MS.gene61472.t1 | MTR_3g107610 | 41.304 | 138 | 49 | 6 | 10 | 118 | 5 | 139 | 2.75e-20 | 82.0 |
| MS.gene61472.t1 | MTR_6g069400 | 29.710 | 138 | 75 | 4 | 1 | 138 | 261 | 376 | 2.64e-14 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61472.t1 | AT2G26695 | 67.857 | 140 | 42 | 2 | 5 | 143 | 1 | 138 | 2.78e-69 | 205 |
| MS.gene61472.t1 | AT2G26695 | 67.857 | 140 | 42 | 2 | 5 | 143 | 1 | 138 | 2.78e-69 | 205 |
| MS.gene61472.t1 | AT3G15680 | 42.857 | 154 | 65 | 5 | 10 | 140 | 5 | 158 | 1.50e-34 | 118 |
| MS.gene61472.t1 | AT3G15680 | 42.857 | 154 | 65 | 5 | 10 | 140 | 5 | 158 | 1.50e-34 | 118 |
| MS.gene61472.t1 | AT5G25490 | 37.888 | 161 | 71 | 4 | 10 | 141 | 5 | 165 | 3.85e-31 | 110 |
| MS.gene61472.t1 | AT5G25490 | 37.888 | 161 | 71 | 4 | 10 | 141 | 5 | 165 | 3.85e-31 | 110 |
| MS.gene61472.t1 | AT2G17975 | 32.886 | 149 | 70 | 5 | 10 | 134 | 7 | 149 | 1.29e-14 | 68.9 |
Find 39 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTTGGGTATCCACAATTT+TGG | 0.191501 | 8.3:+13456523 | None:intergenic |
| GATCATTATTCTTCTGGATA+TGG | 0.206689 | 8.3:-13456363 | MS.gene61472:CDS |
| TTTAAAGATCATTATTCTTC+TGG | 0.213538 | 8.3:-13456369 | MS.gene61472:CDS |
| TGCAAAATGCCAAGGGATTA+TGG | 0.251675 | 8.3:-13455636 | MS.gene61472:intron |
| TTCAGTCCTGTTATATCTAT+AGG | 0.338196 | 8.3:+13456479 | None:intergenic |
| ATCATTATTCTTCTGGATAT+GGG | 0.365982 | 8.3:-13456362 | MS.gene61472:CDS |
| AATTGTGGATACCCAAAGTA+TGG | 0.373044 | 8.3:-13456519 | MS.gene61472:CDS |
| TATCTATAGGTTGATGGGTC+AGG | 0.380599 | 8.3:+13456492 | None:intergenic |
| GGTCAGGGCCTCCATACTTT+GGG | 0.383448 | 8.3:+13456508 | None:intergenic |
| GGGTCAGGGCCTCCATACTT+TGG | 0.410327 | 8.3:+13456507 | None:intergenic |
| ATGGTGACTTCTGGAGGATA+TGG | 0.413606 | 8.3:-13456333 | MS.gene61472:CDS |
| CACATGTTTCCACAATGAAC+AGG | 0.417043 | 8.3:-13456620 | None:intergenic |
| AACAGGAAAATGAGCTGGTC+TGG | 0.419264 | 8.3:-13456603 | MS.gene61472:CDS |
| CATCAACCTATAGATATAAC+AGG | 0.431667 | 8.3:-13456485 | MS.gene61472:CDS |
| CTGTTATATCTATAGGTTGA+TGG | 0.432543 | 8.3:+13456486 | None:intergenic |
| CTGAAACGTTGGCAGGGGAC+TGG | 0.496040 | 8.3:-13456461 | MS.gene61472:CDS |
| AACAGGACTGAAACGTTGGC+AGG | 0.506879 | 8.3:-13456468 | MS.gene61472:CDS |
| TGAGCTGGTCTGGAGGAGAT+TGG | 0.515298 | 8.3:-13456593 | MS.gene61472:CDS |
| TGTTATATCTATAGGTTGAT+GGG | 0.518610 | 8.3:+13456487 | None:intergenic |
| CACATCTATAGCAGTTTGAT+CGG | 0.536065 | 8.3:+13456397 | None:intergenic |
| ATCTATAGGTTGATGGGTCA+GGG | 0.537334 | 8.3:+13456493 | None:intergenic |
| CAGGATGGAAAAGTGGAGAC+TGG | 0.542885 | 8.3:-13456290 | MS.gene61472:CDS |
| TCATTATTCTTCTGGATATG+GGG | 0.550458 | 8.3:-13456361 | MS.gene61472:CDS |
| AGGAAAATGAGCTGGTCTGG+AGG | 0.557211 | 8.3:-13456600 | MS.gene61472:CDS |
| GTGGAGACTGGATTTGCCCT+AGG | 0.558224 | 8.3:-13456278 | MS.gene61472:intron |
| AGAGAAGCATGCCAAAATTG+TGG | 0.558332 | 8.3:-13456534 | MS.gene61472:CDS |
| ACAGGACTGAAACGTTGGCA+GGG | 0.573031 | 8.3:-13456467 | MS.gene61472:CDS |
| ATATAACAGGACTGAAACGT+TGG | 0.578735 | 8.3:-13456472 | MS.gene61472:CDS |
| CAATGAACAGGAAAATGAGC+TGG | 0.583001 | 8.3:-13456608 | MS.gene61472:CDS |
| GGTAACATGGTGACTTCTGG+AGG | 0.587858 | 8.3:-13456339 | MS.gene61472:CDS |
| CAGGACTGAAACGTTGGCAG+GGG | 0.599861 | 8.3:-13456466 | MS.gene61472:CDS |
| GCTACAAATGCAAAATGCCA+AGG | 0.603374 | 8.3:-13455644 | MS.gene61472:CDS |
| CATTATTCTTCTGGATATGG+GGG | 0.604836 | 8.3:-13456360 | MS.gene61472:CDS |
| GAATGCATAATTATGCAAGC+AGG | 0.627959 | 8.3:-13455674 | MS.gene61472:CDS |
| CGATCAAACTGCTATAGATG+TGG | 0.635461 | 8.3:-13456396 | MS.gene61472:CDS |
| TCATGAAGCAGAATTGGCTG+TGG | 0.639985 | 8.3:-13455696 | MS.gene61472:intron |
| TCTGGAGGAGATTGGATGTG+TGG | 0.641624 | 8.3:-13456585 | MS.gene61472:CDS |
| CTACAAATGCAAAATGCCAA+GGG | 0.651877 | 8.3:-13455643 | MS.gene61472:CDS |
| TGTGGATACCCAAAGTATGG+AGG | 0.657966 | 8.3:-13456516 | MS.gene61472:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTATTACTTATATATCAT+TGG | - | chr8.3:13456559-13456578 | MS.gene61472:CDS | 10.0% |
| !! | ATAAAAAAGTAAATAAAACA+AGG | + | chr8.3:13456353-13456372 | None:intergenic | 10.0% |
| !!! | ATCATCATAATTATTATTTT+TGG | + | chr8.3:13456253-13456272 | None:intergenic | 10.0% |
| !! | AATGATTAAGATTAAGAATA+AGG | - | chr8.3:13456185-13456204 | MS.gene61472:intron | 15.0% |
| !!! | TCTTAATCTTAATCATTTTA+TGG | + | chr8.3:13456182-13456201 | None:intergenic | 15.0% |
| !! | AATTATGATGATAATACAGA+TGG | - | chr8.3:13456261-13456280 | MS.gene61472:intron | 20.0% |
| !! | CTGTATTGTAATTATCAATA+TGG | + | chr8.3:13456212-13456231 | None:intergenic | 20.0% |
| !! | TAGAGATAGTTATATATCAT+TGG | - | chr8.3:13456527-13456546 | MS.gene61472:CDS | 20.0% |
| !! | TTATTACTATTGTTTGTTTC+AGG | - | chr8.3:13456593-13456612 | MS.gene61472:CDS | 20.0% |
| !! | TTTAAAGATCATTATTCTTC+TGG | - | chr8.3:13455701-13455720 | MS.gene61472:intron | 20.0% |
| ! | AAAACAAGGATACTCAAATT+AGG | + | chr8.3:13456339-13456358 | None:intergenic | 25.0% |
| ! | AAACAAGGATACTCAAATTA+GGG | + | chr8.3:13456338-13456357 | None:intergenic | 25.0% |
| ! | AATATGTCAACTGTCATAAA+AGG | - | chr8.3:13455978-13455997 | MS.gene61472:intron | 25.0% |
| ! | ACAAATCACAAAGTTCTATA+AGG | + | chr8.3:13455849-13455868 | None:intergenic | 25.0% |
| ! | ATCATTATTCTTCTGGATAT+GGG | - | chr8.3:13455708-13455727 | MS.gene61472:intron | 25.0% |
| !! | TATGACAGTTGACATATTTT+TGG | + | chr8.3:13455976-13455995 | None:intergenic | 25.0% |
| !!! | TGTTATATCTATAGGTTGAT+GGG | + | chr8.3:13455586-13455605 | None:intergenic | 25.0% |
| AAGATAATGTGAACTAACCT+AGG | + | chr8.3:13455812-13455831 | None:intergenic | 30.0% | |
| AGATAATGTGAACTAACCTA+GGG | + | chr8.3:13455811-13455830 | None:intergenic | 30.0% | |
| CATCAACCTATAGATATAAC+AGG | - | chr8.3:13455585-13455604 | MS.gene61472:intron | 30.0% | |
| GAGAAGGATAGGATTATAAT+TGG | - | chr8.3:13456092-13456111 | MS.gene61472:intron | 30.0% | |
| GATAATTACAATACAGACAG+TGG | - | chr8.3:13456216-13456235 | MS.gene61472:intron | 30.0% | |
| GATCATTATTCTTCTGGATA+TGG | - | chr8.3:13455707-13455726 | MS.gene61472:intron | 30.0% | |
| TACTCAAATTAGGGAAGAAT+TGG | + | chr8.3:13456329-13456348 | None:intergenic | 30.0% | |
| TATGATGATAATACAGATGG+TGG | - | chr8.3:13456264-13456283 | MS.gene61472:intron | 30.0% | |
| TCATTATTCTTCTGGATATG+GGG | - | chr8.3:13455709-13455728 | MS.gene61472:intron | 30.0% | |
| TCTATAAGGACTCAAACTAT+AGG | + | chr8.3:13455835-13455854 | None:intergenic | 30.0% | |
| TTACTATTGTTTGTTTCAGG+CGG | - | chr8.3:13456596-13456615 | MS.gene61472:CDS | 30.0% | |
| TTATAATCCTATCCTTCTCT+CGG | + | chr8.3:13456091-13456110 | None:intergenic | 30.0% | |
| TTCAGTCCTGTTATATCTAT+AGG | + | chr8.3:13455594-13455613 | None:intergenic | 30.0% | |
| TTTATGTCATGAAGCAGAAT+TGG | - | chr8.3:13456368-13456387 | MS.gene61472:CDS | 30.0% | |
| ! | CTGAGAAAAAACACTTTTAG+AGG | + | chr8.3:13455906-13455925 | None:intergenic | 30.0% |
| ! | CTGTTATATCTATAGGTTGA+TGG | + | chr8.3:13455587-13455606 | None:intergenic | 30.0% |
| !!! | CACTATGTGTTACTTTTCAA+GGG | + | chr8.3:13456118-13456137 | None:intergenic | 30.0% |
| !!! | TCACTATGTGTTACTTTTCA+AGG | + | chr8.3:13456119-13456138 | None:intergenic | 30.0% |
| AATTGTGGATACCCAAAGTA+TGG | - | chr8.3:13455551-13455570 | MS.gene61472:intron | 35.0% | |
| ATATAACAGGACTGAAACGT+TGG | - | chr8.3:13455598-13455617 | MS.gene61472:intron | 35.0% | |
| CACATCTATAGCAGTTTGAT+CGG | + | chr8.3:13455676-13455695 | None:intergenic | 35.0% | |
| CATTATTCTTCTGGATATGG+GGG | - | chr8.3:13455710-13455729 | MS.gene61472:intron | 35.0% | |
| CTACAAATGCAAAATGCCAA+GGG | - | chr8.3:13456427-13456446 | MS.gene61472:CDS | 35.0% | |
| GAATGCATAATTATGCAAGC+AGG | - | chr8.3:13456396-13456415 | MS.gene61472:CDS | 35.0% | |
| GAGATTGCACTTTCAATGAA+TGG | + | chr8.3:13456057-13456076 | None:intergenic | 35.0% | |
| ! | ACTTTGGGTATCCACAATTT+TGG | + | chr8.3:13455550-13455569 | None:intergenic | 35.0% |
| !!! | AAGAGTGCAGTATTTTTTGC+AGG | - | chr8.3:13456150-13456169 | MS.gene61472:intron | 35.0% |
| !!! | TGTTTTTTCTCAGCTGCATT+TGG | - | chr8.3:13455913-13455932 | MS.gene61472:intron | 35.0% |
| CGATCAAACTGCTATAGATG+TGG | - | chr8.3:13455674-13455693 | MS.gene61472:CDS | 40.0% | |
| GCTACAAATGCAAAATGCCA+AGG | - | chr8.3:13456426-13456445 | MS.gene61472:CDS | 40.0% | |
| TGCAAAATGCCAAGGGATTA+TGG | - | chr8.3:13456434-13456453 | MS.gene61472:CDS | 40.0% | |
| ! | AGAGAAGCATGCCAAAATTG+TGG | - | chr8.3:13455536-13455555 | MS.gene61472:intron | 40.0% |
| ! | CTTTTCTCACCATAATCCCT+TGG | + | chr8.3:13456446-13456465 | None:intergenic | 40.0% |
| ! | TTTTGCACTGCTATGAACTG+TGG | - | chr8.3:13455632-13455651 | MS.gene61472:intron | 40.0% |
| !! | ATCTATAGGTTGATGGGTCA+GGG | + | chr8.3:13455580-13455599 | None:intergenic | 40.0% |
| !! | TATCTATAGGTTGATGGGTC+AGG | + | chr8.3:13455581-13455600 | None:intergenic | 40.0% |
| AACAGGAAAATGAGCTGGTC+TGG | - | chr8.3:13455467-13455486 | MS.gene61472:intron | 45.0% | |
| TAATCCTATCCTTCTCTCGG+AGG | + | chr8.3:13456088-13456107 | None:intergenic | 45.0% | |
| TCATGAAGCAGAATTGGCTG+TGG | - | chr8.3:13456374-13456393 | MS.gene61472:CDS | 45.0% | |
| TGTGGATACCCAAAGTATGG+AGG | - | chr8.3:13455554-13455573 | MS.gene61472:intron | 45.0% | |
| TTCTGGATATGGGGGTAACA+TGG | - | chr8.3:13455718-13455737 | MS.gene61472:intron | 45.0% | |
| ! | ATGGTGACTTCTGGAGGATA+TGG | - | chr8.3:13455737-13455756 | MS.gene61472:intron | 45.0% |
| !! | AGTCTCCACTTTTCCATCCT+GGG | + | chr8.3:13455781-13455800 | None:intergenic | 45.0% |
| AACAGGACTGAAACGTTGGC+AGG | - | chr8.3:13455602-13455621 | MS.gene61472:intron | 50.0% | |
| ACAGGACTGAAACGTTGGCA+GGG | - | chr8.3:13455603-13455622 | MS.gene61472:intron | 50.0% | |
| CAGGATGGAAAAGTGGAGAC+TGG | - | chr8.3:13455780-13455799 | MS.gene61472:intron | 50.0% | |
| CCTTCTCTCGGAGGAAGAAA+AGG | + | chr8.3:13456079-13456098 | None:intergenic | 50.0% | |
| GGTAACATGGTGACTTCTGG+AGG | - | chr8.3:13455731-13455750 | MS.gene61472:intron | 50.0% | |
| TCTGGAGGAGATTGGATGTG+TGG | - | chr8.3:13455485-13455504 | MS.gene61472:intron | 50.0% | |
| TCTTCCTCCGAGAGAAGGAT+AGG | - | chr8.3:13456081-13456100 | MS.gene61472:intron | 50.0% | |
| ! | AGGAAAATGAGCTGGTCTGG+AGG | - | chr8.3:13455470-13455489 | MS.gene61472:intron | 50.0% |
| ! | CCTTTTCTTCCTCCGAGAGA+AGG | - | chr8.3:13456076-13456095 | MS.gene61472:intron | 50.0% |
| !! | CAGTCTCCACTTTTCCATCC+TGG | + | chr8.3:13455782-13455801 | None:intergenic | 50.0% |
| !! | GTCTCCACTTTTCCATCCTG+GGG | + | chr8.3:13455780-13455799 | None:intergenic | 50.0% |
| !! | TCTCCACTTTTCCATCCTGG+GGG | + | chr8.3:13455779-13455798 | None:intergenic | 50.0% |
| ACTGTAGTTCTCCCCCAGGA+TGG | - | chr8.3:13455765-13455784 | MS.gene61472:intron | 55.0% | |
| CAGGACTGAAACGTTGGCAG+GGG | - | chr8.3:13455604-13455623 | MS.gene61472:intron | 55.0% | |
| GGGGGTAACATGGTGACTTC+TGG | - | chr8.3:13455728-13455747 | MS.gene61472:intron | 55.0% | |
| GGTCAGGGCCTCCATACTTT+GGG | + | chr8.3:13455565-13455584 | None:intergenic | 55.0% | |
| GTGGAGACTGGATTTGCCCT+AGG | - | chr8.3:13455792-13455811 | MS.gene61472:intron | 55.0% | |
| TCAGACTGTAGTTCTCCCCC+AGG | - | chr8.3:13455761-13455780 | MS.gene61472:intron | 55.0% | |
| TCTCCCCCAGGATGGAAAAG+TGG | - | chr8.3:13455773-13455792 | MS.gene61472:intron | 55.0% | |
| ! | TGAGCTGGTCTGGAGGAGAT+TGG | - | chr8.3:13455477-13455496 | MS.gene61472:intron | 55.0% |
| CTGAAACGTTGGCAGGGGAC+TGG | - | chr8.3:13455609-13455628 | MS.gene61472:intron | 60.0% | |
| GGGTCAGGGCCTCCATACTT+TGG | + | chr8.3:13455566-13455585 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 13455464 | 13456628 | 13455464 | ID=MS.gene61472 |
| chr8.3 | mRNA | 13455464 | 13456628 | 13455464 | ID=MS.gene61472.t1;Parent=MS.gene61472 |
| chr8.3 | exon | 13456279 | 13456628 | 13456279 | ID=MS.gene61472.t1.exon1;Parent=MS.gene61472.t1 |
| chr8.3 | CDS | 13456279 | 13456628 | 13456279 | ID=cds.MS.gene61472.t1;Parent=MS.gene61472.t1 |
| chr8.3 | exon | 13455637 | 13455707 | 13455637 | ID=MS.gene61472.t1.exon2;Parent=MS.gene61472.t1 |
| chr8.3 | CDS | 13455637 | 13455707 | 13455637 | ID=cds.MS.gene61472.t1;Parent=MS.gene61472.t1 |
| chr8.3 | exon | 13455464 | 13455477 | 13455464 | ID=MS.gene61472.t1.exon3;Parent=MS.gene61472.t1 |
| chr8.3 | CDS | 13455464 | 13455477 | 13455464 | ID=cds.MS.gene61472.t1;Parent=MS.gene61472.t1 |
| Gene Sequence |
| Protein sequence |
