AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072918


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072918.t1 MTR_3g107610 97.076 171 4 1 1 171 1 170 2.76e-108 306
MS.gene072918.t1 MTR_3g107610 90.789 152 12 2 1 152 1 150 2.53e-85 248
MS.gene072918.t1 MTR_4g011190 61.111 162 47 4 1 162 1 146 2.09e-55 172
MS.gene072918.t1 MTR_4g113840 43.030 165 51 6 5 161 6 135 4.59e-33 114
MS.gene072918.t1 MTR_2g054750 36.943 157 58 3 5 160 6 122 8.07e-31 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072918.t1 AT3G15680 60.819 171 58 5 1 169 1 164 4.32e-61 187
MS.gene072918.t1 AT3G15680 60.819 171 58 5 1 169 1 164 4.32e-61 187
MS.gene072918.t1 AT5G25490 62.791 172 50 5 1 164 1 166 1.37e-55 173
MS.gene072918.t1 AT5G25490 62.791 172 50 5 1 164 1 166 1.37e-55 173
MS.gene072918.t1 AT2G26695 40.764 157 65 5 5 161 6 134 1.99e-32 113
MS.gene072918.t1 AT2G26695 40.764 157 65 5 5 161 6 134 1.99e-32 113
MS.gene072918.t1 AT2G17975 30.769 169 80 6 3 157 5 150 4.56e-16 73.9

Find 71 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGGCGGCGCCGTTGATTT+TGG 0.178200 3.4:-93460477 MS.gene072918:CDS
TTGAGATCACCATACGGTTT+TGG 0.292602 3.4:-93460255 MS.gene072918:CDS
GGGGAGTCAAGAATGATTTC+TGG 0.322815 3.4:-93460501 MS.gene072918:CDS
TTGAGAAGTTCACGATAAAT+AGG 0.332528 3.4:+93459780 None:intergenic
TCGGTAGCATTCCATTCTAT+TGG 0.379779 3.4:+93459840 None:intergenic
GGCTCCTTTCTTGGTGGAAG+AGG 0.386495 3.4:-93460453 MS.gene072918:CDS
GAGAAGGTATCAATATCCTT+AGG 0.387497 3.4:+93460306 None:intergenic
ACTCGCCCCGGCTGGAAATC+CGG 0.405258 3.4:-93460204 MS.gene072918:CDS
GTGCCTCCACTCGCCCCGGC+TGG 0.416254 3.4:-93460212 MS.gene072918:CDS
GGGCCGGTGGTGAAAGGAAA+AGG 0.421977 3.4:+93460420 None:intergenic
CAGTCACCAGGACGGACATC+AGG 0.429559 3.4:+93460399 None:intergenic
CTGATGTCCGTCCTGGTGAC+TGG 0.431260 3.4:-93460398 MS.gene072918:CDS
AGATGAGCAGACCAGGAGAT+TGG 0.434758 3.4:-93460581 MS.gene072918:CDS
GTCCTGCAGTTCCAATCTCC+TGG 0.444104 3.4:+93460570 None:intergenic
CTCTTCTTTGAAAGTTGAGG+TGG 0.445847 3.4:+93460541 None:intergenic
GGCACCACATTTGAAGCAGC+TGG 0.455963 3.4:+93460329 None:intergenic
GGAGGAGGCAGCGCCGGTGG+TGG 0.457413 3.4:-93460234 MS.gene072918:CDS
TTTGAAGCAGCTGGAGCGGC+TGG 0.457465 3.4:+93460338 None:intergenic
CGCTCCAGCTGCTTCAAATG+TGG 0.474359 3.4:-93460333 MS.gene072918:CDS
CTTCAAATGTGGTGCCCCTA+AGG 0.478199 3.4:-93460322 MS.gene072918:CDS
CAGTCACCGGATTTCCAGCC+GGG 0.479965 3.4:+93460198 None:intergenic
CCGGCTGGAAATCCGGTGAC+TGG 0.485260 3.4:-93460197 MS.gene072918:CDS
AGAGCCTCTTCCACCAAGAA+AGG 0.496788 3.4:+93460449 None:intergenic
GCATAACTTCGCCAATAGAA+TGG 0.497407 3.4:-93459851 MS.gene072918:CDS
CTTCCAGTACTAGATTCCCT+TGG 0.497781 3.4:+93459808 None:intergenic
ATTCTCTTCTTTGAAAGTTG+AGG 0.498262 3.4:+93460538 None:intergenic
TTTGGTGGCTCCTTTCTTGG+TGG 0.500076 3.4:-93460459 MS.gene072918:CDS
AGCAGAAAGATGAGCAGACC+AGG 0.504369 3.4:-93460588 None:intergenic
CACATTTGAAGCAGCTGGAG+CGG 0.504933 3.4:+93460334 None:intergenic
GGCCGGTGGTGAAAGGAAAA+GGG 0.509497 3.4:+93460421 None:intergenic
AGAAGGTATCAATATCCTTA+GGG 0.509984 3.4:+93460307 None:intergenic
GACCAGGAGATTGGAACTGC+AGG 0.511105 3.4:-93460572 MS.gene072918:CDS
GCTACCGATGCAACGGTCCA+AGG 0.513927 3.4:-93459825 MS.gene072918:CDS
GGATTTCCAGCCGGGGCGAG+TGG 0.514801 3.4:+93460206 None:intergenic
AGAATGATTTCTGGCGGCGG+CGG 0.516918 3.4:-93460492 MS.gene072918:CDS
GCGAGTGGAGGCACCACCAC+CGG 0.520780 3.4:+93460221 None:intergenic
GTCTGAGGAGTCAGAGGAGA+AGG 0.523791 3.4:+93460290 None:intergenic
CCAGTCACCGGATTTCCAGC+CGG 0.530047 3.4:+93460197 None:intergenic
TTCTTGACTCCCCACATCGT+TGG 0.531867 3.4:+93460511 None:intergenic
ACCGGCCCTGATGTCCGTCC+TGG 0.532481 3.4:-93460405 MS.gene072918:CDS
GGTCCAAGGGAATCTAGTAC+TGG 0.534715 3.4:-93459811 MS.gene072918:CDS
TTTGGAGGAGGCAGCGCCGG+TGG 0.535377 3.4:-93460237 MS.gene072918:CDS
GAGAATCTTGCCAACGATGT+GGG 0.536610 3.4:-93460521 MS.gene072918:CDS
GGTGACTGGTATTGTACTGT+TGG 0.542639 3.4:-93460384 MS.gene072918:CDS
ACATCAGGGCCGGTGGTGAA+AGG 0.548866 3.4:+93460414 None:intergenic
ATTCCCTTGGACCGTTGCAT+CGG 0.549976 3.4:+93459821 None:intergenic
TTTCCAGCCGGGGCGAGTGG+AGG 0.551998 3.4:+93460209 None:intergenic
CCGGTGACTGGATATGCACC+AGG 0.562370 3.4:-93460185 MS.gene072918:intron
CTGCCTCCTCCAAAACCGTA+TGG 0.574316 3.4:+93460246 None:intergenic
ACCAGGACGGACATCAGGGC+CGG 0.576233 3.4:+93460404 None:intergenic
AAAGGAGCCACCAAAATCAA+CGG 0.591777 3.4:+93460467 None:intergenic
CTACCGATGCAACGGTCCAA+GGG 0.596798 3.4:-93459824 MS.gene072918:CDS
GGTGGTGCCTCCACTCGCCC+CGG 0.597153 3.4:-93460216 MS.gene072918:CDS
CCTGGTGCATATCCAGTCAC+CGG 0.599174 3.4:+93460185 None:intergenic
AGAGAATCTTGCCAACGATG+TGG 0.600788 3.4:-93460522 MS.gene072918:CDS
AGAATCTTGCCAACGATGTG+GGG 0.604150 3.4:-93460520 MS.gene072918:CDS
CAATAATCGTGGCATGTCTG+AGG 0.609735 3.4:+93460275 None:intergenic
TGGCATGTCTGAGGAGTCAG+AGG 0.612841 3.4:+93460284 None:intergenic
AGGACGGACATCAGGGCCGG+TGG 0.617823 3.4:+93460407 None:intergenic
TCAAGAATGATTTCTGGCGG+CGG 0.619694 3.4:-93460495 MS.gene072918:CDS
ATGGAATGCTACCGATGCAA+CGG 0.621720 3.4:-93459832 MS.gene072918:CDS
TATTGTACTGTTGGAAACTG+TGG 0.623871 3.4:-93460375 MS.gene072918:CDS
CGATTATTGAGATCACCATA+CGG 0.625229 3.4:-93460261 MS.gene072918:CDS
AGTCACCAGGACGGACATCA+GGG 0.629819 3.4:+93460400 None:intergenic
GCCGGTGGTGAAAGGAAAAG+GGG 0.632818 3.4:+93460422 None:intergenic
ACAGTACAATACCAGTCACC+AGG 0.642353 3.4:+93460387 None:intergenic
GAGTCAAGAATGATTTCTGG+CGG 0.656995 3.4:-93460498 MS.gene072918:CDS
TACAATACCAGTCACCAGGA+CGG 0.657561 3.4:+93460391 None:intergenic
TATGGTGATCTCAATAATCG+TGG 0.671531 3.4:+93460264 None:intergenic
GAAGGTATCAATATCCTTAG+GGG 0.684912 3.4:+93460308 None:intergenic
AGTCACCGGATTTCCAGCCG+GGG 0.690621 3.4:+93460199 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATTATAAGTTCCAAATT+AGG + chr3.4:93460251-93460270 None:intergenic 15.0%
!! CTAACAAAATCTCATAAAAA+TGG - chr3.4:93460347-93460366 MS.gene072918:CDS 20.0%
!! GATACTTCTTATAAATATTG+TGG - chr3.4:93460318-93460337 MS.gene072918:CDS 20.0%
!! TTTCATTTGTCTCATTATAA+GGG + chr3.4:93460447-93460466 None:intergenic 20.0%
!!! CATTTTTATGAGATTTTGTT+AGG + chr3.4:93460349-93460368 None:intergenic 20.0%
! CTTCTTATAAATATTGTGGT+TGG - chr3.4:93460322-93460341 MS.gene072918:CDS 25.0%
! GTTTCATTTGTCTCATTATA+AGG + chr3.4:93460448-93460467 None:intergenic 25.0%
ACATGACACTTATAATGAGA+CGG + chr3.4:93460292-93460311 None:intergenic 30.0%
! ATTCTCTTCTTTGAAAGTTG+AGG + chr3.4:93459833-93459852 None:intergenic 30.0%
!! AGAAGGTATCAATATCCTTA+GGG + chr3.4:93460064-93460083 None:intergenic 30.0%
AGAATTGAAATGTGTAGGTC+TGG - chr3.4:93460485-93460504 MS.gene072918:CDS 35.0%
CGATTATTGAGATCACCATA+CGG - chr3.4:93460107-93460126 MS.gene072918:intron 35.0%
GAAATGATTGACATGAAACC+TGG + chr3.4:93460204-93460223 None:intergenic 35.0%
GAATTGAAATGTGTAGGTCT+GGG - chr3.4:93460486-93460505 MS.gene072918:CDS 35.0%
GACCTACACATTTCAATTCT+CGG + chr3.4:93460485-93460504 None:intergenic 35.0%
TACCGAGAATTGAAATGTGT+AGG - chr3.4:93460480-93460499 MS.gene072918:CDS 35.0%
TATGGTGATCTCAATAATCG+TGG + chr3.4:93460107-93460126 None:intergenic 35.0%
TATTGTACTGTTGGAAACTG+TGG - chr3.4:93459993-93460012 MS.gene072918:intron 35.0%
!! GAAGGTATCAATATCCTTAG+GGG + chr3.4:93460063-93460082 None:intergenic 35.0%
!! GAGAAGGTATCAATATCCTT+AGG + chr3.4:93460065-93460084 None:intergenic 35.0%
AAAGGAGCCACCAAAATCAA+CGG + chr3.4:93459904-93459923 None:intergenic 40.0%
CAAATTAGGACGAAGTAGCA+AGG + chr3.4:93460237-93460256 None:intergenic 40.0%
CAATTCTCGGTAATGTCAGT+AGG + chr3.4:93460472-93460491 None:intergenic 40.0%
GCATAACTTCGCCAATAGAA+TGG - chr3.4:93460517-93460536 MS.gene072918:CDS 40.0%
TCGGTAGCATTCCATTCTAT+TGG + chr3.4:93460531-93460550 None:intergenic 40.0%
! CTCTTCTTTGAAAGTTGAGG+TGG + chr3.4:93459830-93459849 None:intergenic 40.0%
! CTTGCTACTTCGTCCTAATT+TGG - chr3.4:93460235-93460254 MS.gene072918:CDS 40.0%
! GAGTCAAGAATGATTTCTGG+CGG - chr3.4:93459870-93459889 MS.gene072918:intron 40.0%
! TTGAGATCACCATACGGTTT+TGG - chr3.4:93460113-93460132 MS.gene072918:intron 40.0%
ACAGTACAATACCAGTCACC+AGG + chr3.4:93459984-93460003 None:intergenic 45.0%
AGAATCTTGCCAACGATGTG+GGG - chr3.4:93459848-93459867 MS.gene072918:CDS 45.0%
AGAGAATCTTGCCAACGATG+TGG - chr3.4:93459846-93459865 MS.gene072918:CDS 45.0%
ATGGAATGCTACCGATGCAA+CGG - chr3.4:93460536-93460555 MS.gene072918:CDS 45.0%
CAATAATCGTGGCATGTCTG+AGG + chr3.4:93460096-93460115 None:intergenic 45.0%
CTTCCAGTACTAGATTCCCT+TGG + chr3.4:93460563-93460582 None:intergenic 45.0%
GAGAATCTTGCCAACGATGT+GGG - chr3.4:93459847-93459866 MS.gene072918:CDS 45.0%
GGGGAGTCAAGAATGATTTC+TGG - chr3.4:93459867-93459886 MS.gene072918:CDS 45.0%
GGTGACTGGTATTGTACTGT+TGG - chr3.4:93459984-93460003 MS.gene072918:intron 45.0%
TACAATACCAGTCACCAGGA+CGG + chr3.4:93459980-93459999 None:intergenic 45.0%
!! AGATCACCATACGGTTTTGG+AGG - chr3.4:93460116-93460135 MS.gene072918:intron 45.0%
!! GATTTTGGTGGCTCCTTTCT+TGG - chr3.4:93459906-93459925 MS.gene072918:intron 45.0%
!! TCAAGAATGATTTCTGGCGG+CGG - chr3.4:93459873-93459892 MS.gene072918:intron 45.0%
AGAGCCTCTTCCACCAAGAA+AGG + chr3.4:93459922-93459941 None:intergenic 50.0%
ATTCCCTTGGACCGTTGCAT+CGG + chr3.4:93460550-93460569 None:intergenic 50.0%
CTTCAAATGTGGTGCCCCTA+AGG - chr3.4:93460046-93460065 MS.gene072918:intron 50.0%
GGTCCAAGGGAATCTAGTAC+TGG - chr3.4:93460557-93460576 MS.gene072918:CDS 50.0%
TTCTTGACTCCCCACATCGT+TGG + chr3.4:93459860-93459879 None:intergenic 50.0%
! CACATTTGAAGCAGCTGGAG+CGG + chr3.4:93460037-93460056 None:intergenic 50.0%
! TCCCCTTTTCCTTTCACCAC+CGG - chr3.4:93459945-93459964 MS.gene072918:intron 50.0%
! TTTGGTGGCTCCTTTCTTGG+TGG - chr3.4:93459909-93459928 MS.gene072918:intron 50.0%
!!! TCACCATACGGTTTTGGAGG+AGG - chr3.4:93460119-93460138 MS.gene072918:intron 50.0%
AGTCACCAGGACGGACATCA+GGG + chr3.4:93459971-93459990 None:intergenic 55.0%
CCTGGTGCATATCCAGTCAC+CGG + chr3.4:93460186-93460205 None:intergenic 55.0%
CGCTCCAGCTGCTTCAAATG+TGG - chr3.4:93460035-93460054 MS.gene072918:intron 55.0%
CTACCGATGCAACGGTCCAA+GGG - chr3.4:93460544-93460563 MS.gene072918:CDS 55.0%
CTGCCTCCTCCAAAACCGTA+TGG + chr3.4:93460125-93460144 None:intergenic 55.0%
GACCAGGAGATTGGAACTGC+AGG - chr3.4:93459796-93459815 MS.gene072918:CDS 55.0%
GCCGGTGGTGAAAGGAAAAG+GGG + chr3.4:93459949-93459968 None:intergenic 55.0%
GGCCGGTGGTGAAAGGAAAA+GGG + chr3.4:93459950-93459969 None:intergenic 55.0%
GGCTCCTTTCTTGGTGGAAG+AGG - chr3.4:93459915-93459934 MS.gene072918:intron 55.0%
GTCCTGCAGTTCCAATCTCC+TGG + chr3.4:93459801-93459820 None:intergenic 55.0%
GTCTGAGGAGTCAGAGGAGA+AGG + chr3.4:93460081-93460100 None:intergenic 55.0%
TGGCATGTCTGAGGAGTCAG+AGG + chr3.4:93460087-93460106 None:intergenic 55.0%
! GGCACCACATTTGAAGCAGC+TGG + chr3.4:93460042-93460061 None:intergenic 55.0%
!! AGAATGATTTCTGGCGGCGG+CGG - chr3.4:93459876-93459895 MS.gene072918:intron 55.0%
ACATCAGGGCCGGTGGTGAA+AGG + chr3.4:93459957-93459976 None:intergenic 60.0%
AGTCACCGGATTTCCAGCCG+GGG + chr3.4:93460172-93460191 None:intergenic 60.0%
CAGTCACCAGGACGGACATC+AGG + chr3.4:93459972-93459991 None:intergenic 60.0%
CAGTCACCGGATTTCCAGCC+GGG + chr3.4:93460173-93460192 None:intergenic 60.0%
CCAGTCACCGGATTTCCAGC+CGG + chr3.4:93460174-93460193 None:intergenic 60.0%
CCGGTGACTGGATATGCACC+AGG - chr3.4:93460183-93460202 MS.gene072918:intron 60.0%
CTGATGTCCGTCCTGGTGAC+TGG - chr3.4:93459970-93459989 MS.gene072918:intron 60.0%
GCTACCGATGCAACGGTCCA+AGG - chr3.4:93460543-93460562 MS.gene072918:CDS 60.0%
GGGCCGGTGGTGAAAGGAAA+AGG + chr3.4:93459951-93459970 None:intergenic 60.0%
! TTTGAAGCAGCTGGAGCGGC+TGG + chr3.4:93460033-93460052 None:intergenic 60.0%
ACCAGGACGGACATCAGGGC+CGG + chr3.4:93459967-93459986 None:intergenic 65.0%
ACTCGCCCCGGCTGGAAATC+CGG - chr3.4:93460164-93460183 MS.gene072918:intron 65.0%
CCGGCTGGAAATCCGGTGAC+TGG - chr3.4:93460171-93460190 MS.gene072918:intron 65.0%
!!! GGCGGCGCCGTTGATTTTGG+TGG - chr3.4:93459894-93459913 MS.gene072918:intron 65.0%
!!! GGTTTTGGAGGAGGCAGCGC+CGG - chr3.4:93460128-93460147 MS.gene072918:intron 65.0%
ACCGGCCCTGATGTCCGTCC+TGG - chr3.4:93459963-93459982 MS.gene072918:intron 70.0%
AGGACGGACATCAGGGCCGG+TGG + chr3.4:93459964-93459983 None:intergenic 70.0%
GGATTTCCAGCCGGGGCGAG+TGG + chr3.4:93460165-93460184 None:intergenic 70.0%
TTTCCAGCCGGGGCGAGTGG+AGG + chr3.4:93460162-93460181 None:intergenic 70.0%
! TTTGGAGGAGGCAGCGCCGG+TGG - chr3.4:93460131-93460150 MS.gene072918:intron 70.0%
!! GCGAGTGGAGGCACCACCAC+CGG + chr3.4:93460150-93460169 None:intergenic 70.0%
!!! GGCGGCGGCGCCGTTGATTT+TGG - chr3.4:93459891-93459910 MS.gene072918:intron 70.0%
GGTGGTGCCTCCACTCGCCC+CGG - chr3.4:93460152-93460171 MS.gene072918:intron 75.0%
! GTGCCTCCACTCGCCCCGGC+TGG - chr3.4:93460156-93460175 MS.gene072918:intron 80.0%
!!! GGAGGAGGCAGCGCCGGTGG+TGG - chr3.4:93460134-93460153 MS.gene072918:intron 80.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 93459789 93460601 93459789 ID=MS.gene072918
chr3.4 mRNA 93459789 93460601 93459789 ID=MS.gene072918.t1;Parent=MS.gene072918
chr3.4 exon 93460186 93460601 93460186 ID=MS.gene072918.t1.exon1;Parent=MS.gene072918.t1
chr3.4 CDS 93460186 93460601 93460186 ID=cds.MS.gene072918.t1;Parent=MS.gene072918.t1
chr3.4 exon 93459789 93459888 93459789 ID=MS.gene072918.t1.exon2;Parent=MS.gene072918.t1
chr3.4 CDS 93459789 93459888 93459789 ID=cds.MS.gene072918.t1;Parent=MS.gene072918.t1
Gene Sequence

>MS.gene072918

ATGAGCAGACCAGGAGATTGGAACTGCAGGACATGCAACCACCTCAACTTTCAAAGAAGAGAATCTTGCCAACGATGTGGGGAGTCAAGAATGATTTCTGGCGGCGGCGGCGCCGTTGATTTTGGTGGCTCCTTTCTTGGTGGAAGAGGCTCTAGCTCCCCTTTTCCTTTCACCACCGGCCCTGATGTCCGTCCTGGTGACTGGTATTGTACTGTTGGAAACTGTGGAGCTCACAACTTTGCCAGCCGCTCCAGCTGCTTCAAATGTGGTGCCCCTAAGGATATTGATACCTTCTCCTCTGACTCCTCAGACATGCCACGATTATTGAGATCACCATACGGTTTTGGAGGAGGCAGCGCCGGTGGTGGTGCCTCCACTCGCCCCGGCTGGAAATCCGGTGACTGGATATGCACCAGGTTTCATGTCAATCATTTCTTTCATTATACCTTGCTACTTCGTCCTAATTTGGAACTTATAATATATACTAGCATATACTTACTCCGTCTCATTATAAGTGTCATGTTAACGTGATACTTCTTATAAATATTGTGGTTGGACCTAACAAAATCTCATAAAAATGGTTTGTAAGTTTCACTTATAAACATGATCATCTCTCATCAAATATGAAACTTTTAACGTGACACTATTAATGCGACCCTTATAATGAGACAAATGAAACTCCTACTGACATTACCGAGAATTGAAATGTGTAGGTCTGGGTGTAACGAGCATAACTTCGCCAATAGAATGGAATGCTACCGATGCAACGGTCCAAGGGAATCTAGTACTGGAAGATCTTCCTATTTATCGTGA

Protein sequence

>MS.gene072918.t1

MSRPGDWNCRTCNHLNFQRRESCQRCGESRMISGGGGAVDFGGSFLGGRGSSSPFPFTTGPDVRPGDWYCTVGNCGAHNFASRSSCFKCGAPKDIDTFSSDSSDMPRLLRSPYGFGGGSAGGGASTRPGWKSGDWICTRSGCNEHNFANRMECYRCNGPRESSTGRSSYLS