AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene014815


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene014815.t1 MTR_3g107610 96.491 171 5 1 1 171 1 170 1.54e-107 304
MS.gene014815.t1 MTR_3g107610 90.132 152 13 2 1 152 1 150 1.28e-84 246
MS.gene014815.t1 MTR_4g011190 61.728 162 46 4 1 162 1 146 4.56e-56 173
MS.gene014815.t1 MTR_4g113840 43.030 165 51 6 5 161 6 135 6.85e-33 114
MS.gene014815.t1 MTR_2g054750 36.306 157 59 3 5 160 6 122 4.38e-30 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene014815.t1 AT3G15680 60.234 171 59 5 1 169 1 164 2.23e-60 185
MS.gene014815.t1 AT3G15680 60.234 171 59 5 1 169 1 164 2.23e-60 185
MS.gene014815.t1 AT5G25490 62.791 172 50 5 1 164 1 166 2.98e-55 172
MS.gene014815.t1 AT5G25490 62.791 172 50 5 1 164 1 166 2.98e-55 172
MS.gene014815.t1 AT2G26695 40.764 157 65 5 5 161 6 134 2.81e-32 112
MS.gene014815.t1 AT2G26695 40.764 157 65 5 5 161 6 134 2.81e-32 112
MS.gene014815.t1 AT2G17975 30.769 169 80 6 3 157 5 150 4.75e-16 73.9

Find 71 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGGCGGCGCCGTTGATTT+TGG 0.178200 3.3:-87970402 MS.gene014815:CDS
TTGAGATCACCATACGGTTT+TGG 0.292602 3.3:-87970180 MS.gene014815:CDS
GGGGAGTCAAGAATGATTTC+TGG 0.322815 3.3:-87970426 MS.gene014815:CDS
TTGAGAAGTTCACGATAAAT+AGG 0.332528 3.3:+87969705 None:intergenic
TCGGTAGCATTCCATTCTAT+TGG 0.379779 3.3:+87969765 None:intergenic
GGCTCCTTTCTTGGTGGAAG+AGG 0.386495 3.3:-87970378 MS.gene014815:CDS
GAGAAGGTATCAATATCCTT+AGG 0.387497 3.3:+87970231 None:intergenic
GGCACCACATTTGAAGCAAC+TGG 0.387865 3.3:+87970254 None:intergenic
ACTCGCCCCGGCTGGAAATC+CGG 0.405258 3.3:-87970129 MS.gene014815:CDS
GTGCCTCCACTCGCCCCGGC+TGG 0.416254 3.3:-87970137 MS.gene014815:CDS
GGGCCGGTGGTGAAAGGAAA+AGG 0.421977 3.3:+87970345 None:intergenic
CAGTCACCAGGACGGACATC+AGG 0.429559 3.3:+87970324 None:intergenic
CTGATGTCCGTCCTGGTGAC+TGG 0.431260 3.3:-87970323 MS.gene014815:CDS
AGATGAGCAGACCAGGAGAT+TGG 0.434758 3.3:-87970506 MS.gene014815:CDS
GTCCTGCAGTTCCAATCTCC+TGG 0.444104 3.3:+87970495 None:intergenic
CTCTTCTTTGAAAGTTGAGG+TGG 0.445847 3.3:+87970466 None:intergenic
GGAGGAGGCAGCGCCGGTGG+TGG 0.457413 3.3:-87970159 MS.gene014815:CDS
CCGGTGACTGGATATGCAAC+AGG 0.466598 3.3:-87970110 MS.gene014815:intron
CAGTCACCGGATTTCCAGCC+GGG 0.479965 3.3:+87970123 None:intergenic
CTTCAAATGTGGTGCCCCTA+AGG 0.480371 3.3:-87970247 MS.gene014815:CDS
CCGGCTGGAAATCCGGTGAC+TGG 0.485260 3.3:-87970122 MS.gene014815:CDS
GCATAACTTCGCCAATAGAA+TGG 0.497407 3.3:-87969776 MS.gene014815:CDS
CTTCCAGTACTAGATTCCCT+TGG 0.497781 3.3:+87969733 None:intergenic
ATTCTCTTCTTTGAAAGTTG+AGG 0.498262 3.3:+87970463 None:intergenic
TTTGGTGGCTCCTTTCTTGG+TGG 0.500076 3.3:-87970384 MS.gene014815:CDS
AGAGCCTCTTCCACCAAGAA+AGG 0.502352 3.3:+87970374 None:intergenic
AGCAGAAAGATGAGCAGACC+AGG 0.504369 3.3:-87970513 None:intergenic
GGCCGGTGGTGAAAGGAAAA+GGG 0.509497 3.3:+87970346 None:intergenic
AGAAGGTATCAATATCCTTA+GGG 0.509984 3.3:+87970232 None:intergenic
GACCAGGAGATTGGAACTGC+AGG 0.511105 3.3:-87970497 MS.gene014815:CDS
GCTACCGATGCAACGGTCCA+AGG 0.513927 3.3:-87969750 MS.gene014815:CDS
GGATTTCCAGCCGGGGCGAG+TGG 0.514801 3.3:+87970131 None:intergenic
AGAATGATTTCTGGCGGCGG+CGG 0.516918 3.3:-87970417 MS.gene014815:CDS
TTTGAAGCAACTGGAGCGGC+TGG 0.520103 3.3:+87970263 None:intergenic
GCGAGTGGAGGCACCACCAC+CGG 0.520780 3.3:+87970146 None:intergenic
GTCTGAGGAGTCAGAGGAGA+AGG 0.523791 3.3:+87970215 None:intergenic
CCAGTCACCGGATTTCCAGC+CGG 0.530047 3.3:+87970122 None:intergenic
TTCTTGACTCCCCACATCGT+TGG 0.531867 3.3:+87970436 None:intergenic
ACCGGCCCTGATGTCCGTCC+TGG 0.532481 3.3:-87970330 MS.gene014815:CDS
GGTCCAAGGGAATCTAGTAC+TGG 0.534715 3.3:-87969736 MS.gene014815:CDS
TTTGGAGGAGGCAGCGCCGG+TGG 0.535377 3.3:-87970162 MS.gene014815:CDS
GAGAATCTTGCCAACGATGT+GGG 0.536610 3.3:-87970446 MS.gene014815:CDS
GGTGACTGGTATTGTACTGT+TGG 0.542639 3.3:-87970309 MS.gene014815:CDS
ACATCAGGGCCGGTGGTGAA+AGG 0.548866 3.3:+87970339 None:intergenic
ATTCCCTTGGACCGTTGCAT+CGG 0.549976 3.3:+87969746 None:intergenic
TTTCCAGCCGGGGCGAGTGG+AGG 0.551998 3.3:+87970134 None:intergenic
CGCTCCAGTTGCTTCAAATG+TGG 0.553538 3.3:-87970258 MS.gene014815:CDS
CTGCCTCCTCCAAAACCGTA+TGG 0.574316 3.3:+87970171 None:intergenic
ACCAGGACGGACATCAGGGC+CGG 0.576233 3.3:+87970329 None:intergenic
AAAGGAGCCACCAAAATCAA+CGG 0.591777 3.3:+87970392 None:intergenic
CTACCGATGCAACGGTCCAA+GGG 0.596798 3.3:-87969749 MS.gene014815:CDS
GGTGGTGCCTCCACTCGCCC+CGG 0.597153 3.3:-87970141 MS.gene014815:CDS
CCTGTTGCATATCCAGTCAC+CGG 0.599174 3.3:+87970110 None:intergenic
AGAGAATCTTGCCAACGATG+TGG 0.600788 3.3:-87970447 MS.gene014815:CDS
AGAATCTTGCCAACGATGTG+GGG 0.604150 3.3:-87970445 MS.gene014815:CDS
CAATAATCGTGGCATGTCTG+AGG 0.609735 3.3:+87970200 None:intergenic
TGGCATGTCTGAGGAGTCAG+AGG 0.612841 3.3:+87970209 None:intergenic
AGGACGGACATCAGGGCCGG+TGG 0.617823 3.3:+87970332 None:intergenic
TCAAGAATGATTTCTGGCGG+CGG 0.619694 3.3:-87970420 MS.gene014815:CDS
ATGGAATGCTACCGATGCAA+CGG 0.621720 3.3:-87969757 MS.gene014815:CDS
TATTGTACTGTTGGAAACTG+TGG 0.623871 3.3:-87970300 MS.gene014815:CDS
CGATTATTGAGATCACCATA+CGG 0.625229 3.3:-87970186 MS.gene014815:CDS
CACATTTGAAGCAACTGGAG+CGG 0.628864 3.3:+87970259 None:intergenic
AGTCACCAGGACGGACATCA+GGG 0.629819 3.3:+87970325 None:intergenic
ACAGTACAATACCAGTCACC+AGG 0.642353 3.3:+87970312 None:intergenic
GAGTCAAGAATGATTTCTGG+CGG 0.656995 3.3:-87970423 MS.gene014815:CDS
TACAATACCAGTCACCAGGA+CGG 0.657561 3.3:+87970316 None:intergenic
GCCGGTGGTGAAAGGAAAAG+GGG 0.658542 3.3:+87970347 None:intergenic
TATGGTGATCTCAATAATCG+TGG 0.671531 3.3:+87970189 None:intergenic
GAAGGTATCAATATCCTTAG+GGG 0.684912 3.3:+87970233 None:intergenic
AGTCACCGGATTTCCAGCCG+GGG 0.690621 3.3:+87970124 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAACAAAATCTCATAAAAA+TGG - chr3.3:87970272-87970291 MS.gene014815:CDS 20.0%
!! GATACTTCTTATAAATATTG+TGG - chr3.3:87970243-87970262 MS.gene014815:CDS 20.0%
!! TTTCATTTGTCTCATTATAA+GGG + chr3.3:87970372-87970391 None:intergenic 20.0%
!!! CATTTTTATGAGATTTTGTT+AGG + chr3.3:87970274-87970293 None:intergenic 20.0%
! CTTCTTATAAATATTGTGGT+TGG - chr3.3:87970247-87970266 MS.gene014815:CDS 25.0%
! GTTTCATTTGTCTCATTATA+AGG + chr3.3:87970373-87970392 None:intergenic 25.0%
ACATGACACTTATAATGAGA+CGG + chr3.3:87970217-87970236 None:intergenic 30.0%
! ATTCTCTTCTTTGAAAGTTG+AGG + chr3.3:87969758-87969777 None:intergenic 30.0%
!! AGAAGGTATCAATATCCTTA+GGG + chr3.3:87969989-87970008 None:intergenic 30.0%
AGAATTGAAATGTGTAGGTC+TGG - chr3.3:87970410-87970429 MS.gene014815:CDS 35.0%
CAAATTAGAACGAAGTAGCA+AGG + chr3.3:87970162-87970181 None:intergenic 35.0%
CGATTATTGAGATCACCATA+CGG - chr3.3:87970032-87970051 MS.gene014815:intron 35.0%
GAATTGAAATGTGTAGGTCT+GGG - chr3.3:87970411-87970430 MS.gene014815:CDS 35.0%
GACCTACACATTTCAATTCT+CGG + chr3.3:87970410-87970429 None:intergenic 35.0%
TACCGAGAATTGAAATGTGT+AGG - chr3.3:87970405-87970424 MS.gene014815:CDS 35.0%
TATGGTGATCTCAATAATCG+TGG + chr3.3:87970032-87970051 None:intergenic 35.0%
TATTGTACTGTTGGAAACTG+TGG - chr3.3:87969918-87969937 MS.gene014815:intron 35.0%
! CTTGCTACTTCGTTCTAATT+TGG - chr3.3:87970160-87970179 MS.gene014815:CDS 35.0%
!! GAAGGTATCAATATCCTTAG+GGG + chr3.3:87969988-87970007 None:intergenic 35.0%
!! GAGAAGGTATCAATATCCTT+AGG + chr3.3:87969990-87970009 None:intergenic 35.0%
AAAGGAGCCACCAAAATCAA+CGG + chr3.3:87969829-87969848 None:intergenic 40.0%
CAATTCTCGGTAATGTCAGT+AGG + chr3.3:87970397-87970416 None:intergenic 40.0%
GCATAACTTCGCCAATAGAA+TGG - chr3.3:87970442-87970461 MS.gene014815:CDS 40.0%
TCGGTAGCATTCCATTCTAT+TGG + chr3.3:87970456-87970475 None:intergenic 40.0%
! CTCTTCTTTGAAAGTTGAGG+TGG + chr3.3:87969755-87969774 None:intergenic 40.0%
! GAGTCAAGAATGATTTCTGG+CGG - chr3.3:87969795-87969814 MS.gene014815:intron 40.0%
! TTGAGATCACCATACGGTTT+TGG - chr3.3:87970038-87970057 MS.gene014815:intron 40.0%
ACAGTACAATACCAGTCACC+AGG + chr3.3:87969909-87969928 None:intergenic 45.0%
AGAATCTTGCCAACGATGTG+GGG - chr3.3:87969773-87969792 MS.gene014815:CDS 45.0%
AGAGAATCTTGCCAACGATG+TGG - chr3.3:87969771-87969790 MS.gene014815:CDS 45.0%
ATGGAATGCTACCGATGCAA+CGG - chr3.3:87970461-87970480 MS.gene014815:CDS 45.0%
CAATAATCGTGGCATGTCTG+AGG + chr3.3:87970021-87970040 None:intergenic 45.0%
CTTCCAGTACTAGATTCCCT+TGG + chr3.3:87970488-87970507 None:intergenic 45.0%
GAGAATCTTGCCAACGATGT+GGG - chr3.3:87969772-87969791 MS.gene014815:CDS 45.0%
GGGGAGTCAAGAATGATTTC+TGG - chr3.3:87969792-87969811 MS.gene014815:CDS 45.0%
GGTGACTGGTATTGTACTGT+TGG - chr3.3:87969909-87969928 MS.gene014815:intron 45.0%
TACAATACCAGTCACCAGGA+CGG + chr3.3:87969905-87969924 None:intergenic 45.0%
! CACATTTGAAGCAACTGGAG+CGG + chr3.3:87969962-87969981 None:intergenic 45.0%
!! AGATCACCATACGGTTTTGG+AGG - chr3.3:87970041-87970060 MS.gene014815:intron 45.0%
!! GATTTTGGTGGCTCCTTTCT+TGG - chr3.3:87969831-87969850 MS.gene014815:intron 45.0%
!! TCAAGAATGATTTCTGGCGG+CGG - chr3.3:87969798-87969817 MS.gene014815:intron 45.0%
AGAGCCTCTTCCACCAAGAA+AGG + chr3.3:87969847-87969866 None:intergenic 50.0%
ATTCCCTTGGACCGTTGCAT+CGG + chr3.3:87970475-87970494 None:intergenic 50.0%
CCTGTTGCATATCCAGTCAC+CGG + chr3.3:87970111-87970130 None:intergenic 50.0%
CGCTCCAGTTGCTTCAAATG+TGG - chr3.3:87969960-87969979 MS.gene014815:intron 50.0%
CTTCAAATGTGGTGCCCCTA+AGG - chr3.3:87969971-87969990 MS.gene014815:intron 50.0%
GGTCCAAGGGAATCTAGTAC+TGG - chr3.3:87970482-87970501 MS.gene014815:CDS 50.0%
TTCTTGACTCCCCACATCGT+TGG + chr3.3:87969785-87969804 None:intergenic 50.0%
! GGCACCACATTTGAAGCAAC+TGG + chr3.3:87969967-87969986 None:intergenic 50.0%
! TCCCCTTTTCCTTTCACCAC+CGG - chr3.3:87969870-87969889 MS.gene014815:intron 50.0%
! TTTGGTGGCTCCTTTCTTGG+TGG - chr3.3:87969834-87969853 MS.gene014815:intron 50.0%
!!! TCACCATACGGTTTTGGAGG+AGG - chr3.3:87970044-87970063 MS.gene014815:intron 50.0%
AGTCACCAGGACGGACATCA+GGG + chr3.3:87969896-87969915 None:intergenic 55.0%
CCGGTGACTGGATATGCAAC+AGG - chr3.3:87970108-87970127 MS.gene014815:intron 55.0%
CTACCGATGCAACGGTCCAA+GGG - chr3.3:87970469-87970488 MS.gene014815:CDS 55.0%
CTGCCTCCTCCAAAACCGTA+TGG + chr3.3:87970050-87970069 None:intergenic 55.0%
GACCAGGAGATTGGAACTGC+AGG - chr3.3:87969721-87969740 MS.gene014815:CDS 55.0%
GCCGGTGGTGAAAGGAAAAG+GGG + chr3.3:87969874-87969893 None:intergenic 55.0%
GGCCGGTGGTGAAAGGAAAA+GGG + chr3.3:87969875-87969894 None:intergenic 55.0%
GGCTCCTTTCTTGGTGGAAG+AGG - chr3.3:87969840-87969859 MS.gene014815:intron 55.0%
GTCCTGCAGTTCCAATCTCC+TGG + chr3.3:87969726-87969745 None:intergenic 55.0%
GTCTGAGGAGTCAGAGGAGA+AGG + chr3.3:87970006-87970025 None:intergenic 55.0%
TGGCATGTCTGAGGAGTCAG+AGG + chr3.3:87970012-87970031 None:intergenic 55.0%
! TTTGAAGCAACTGGAGCGGC+TGG + chr3.3:87969958-87969977 None:intergenic 55.0%
!! AGAATGATTTCTGGCGGCGG+CGG - chr3.3:87969801-87969820 MS.gene014815:intron 55.0%
ACATCAGGGCCGGTGGTGAA+AGG + chr3.3:87969882-87969901 None:intergenic 60.0%
AGTCACCGGATTTCCAGCCG+GGG + chr3.3:87970097-87970116 None:intergenic 60.0%
CAGTCACCAGGACGGACATC+AGG + chr3.3:87969897-87969916 None:intergenic 60.0%
CAGTCACCGGATTTCCAGCC+GGG + chr3.3:87970098-87970117 None:intergenic 60.0%
CCAGTCACCGGATTTCCAGC+CGG + chr3.3:87970099-87970118 None:intergenic 60.0%
CTGATGTCCGTCCTGGTGAC+TGG - chr3.3:87969895-87969914 MS.gene014815:intron 60.0%
GCTACCGATGCAACGGTCCA+AGG - chr3.3:87970468-87970487 MS.gene014815:CDS 60.0%
GGGCCGGTGGTGAAAGGAAA+AGG + chr3.3:87969876-87969895 None:intergenic 60.0%
ACCAGGACGGACATCAGGGC+CGG + chr3.3:87969892-87969911 None:intergenic 65.0%
ACTCGCCCCGGCTGGAAATC+CGG - chr3.3:87970089-87970108 MS.gene014815:intron 65.0%
CCGGCTGGAAATCCGGTGAC+TGG - chr3.3:87970096-87970115 MS.gene014815:intron 65.0%
!!! GGCGGCGCCGTTGATTTTGG+TGG - chr3.3:87969819-87969838 MS.gene014815:intron 65.0%
!!! GGTTTTGGAGGAGGCAGCGC+CGG - chr3.3:87970053-87970072 MS.gene014815:intron 65.0%
ACCGGCCCTGATGTCCGTCC+TGG - chr3.3:87969888-87969907 MS.gene014815:intron 70.0%
AGGACGGACATCAGGGCCGG+TGG + chr3.3:87969889-87969908 None:intergenic 70.0%
GGATTTCCAGCCGGGGCGAG+TGG + chr3.3:87970090-87970109 None:intergenic 70.0%
TTTCCAGCCGGGGCGAGTGG+AGG + chr3.3:87970087-87970106 None:intergenic 70.0%
! TTTGGAGGAGGCAGCGCCGG+TGG - chr3.3:87970056-87970075 MS.gene014815:intron 70.0%
!! GCGAGTGGAGGCACCACCAC+CGG + chr3.3:87970075-87970094 None:intergenic 70.0%
!!! GGCGGCGGCGCCGTTGATTT+TGG - chr3.3:87969816-87969835 MS.gene014815:intron 70.0%
GGTGGTGCCTCCACTCGCCC+CGG - chr3.3:87970077-87970096 MS.gene014815:intron 75.0%
! GTGCCTCCACTCGCCCCGGC+TGG - chr3.3:87970081-87970100 MS.gene014815:intron 80.0%
!!! GGAGGAGGCAGCGCCGGTGG+TGG - chr3.3:87970059-87970078 MS.gene014815:intron 80.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 87969714 87970526 87969714 ID=MS.gene014815
chr3.3 mRNA 87969714 87970526 87969714 ID=MS.gene014815.t1;Parent=MS.gene014815
chr3.3 exon 87970111 87970526 87970111 ID=MS.gene014815.t1.exon1;Parent=MS.gene014815.t1
chr3.3 CDS 87970111 87970526 87970111 ID=cds.MS.gene014815.t1;Parent=MS.gene014815.t1
chr3.3 exon 87969714 87969813 87969714 ID=MS.gene014815.t1.exon2;Parent=MS.gene014815.t1
chr3.3 CDS 87969714 87969813 87969714 ID=cds.MS.gene014815.t1;Parent=MS.gene014815.t1
Gene Sequence

>MS.gene014815

ATGAGCAGACCAGGAGATTGGAACTGCAGGACATGCAACCACCTCAACTTTCAAAGAAGAGAATCTTGCCAACGATGTGGGGAGTCAAGAATGATTTCTGGCGGCGGCGGCGCCGTTGATTTTGGTGGCTCCTTTCTTGGTGGAAGAGGCTCTAGTTCCCCTTTTCCTTTCACCACCGGCCCTGATGTCCGTCCTGGTGACTGGTATTGTACTGTTGGAAACTGTGGAGCTCACAACTTTGCCAGCCGCTCCAGTTGCTTCAAATGTGGTGCCCCTAAGGATATTGATACCTTCTCCTCTGACTCCTCAGACATGCCACGATTATTGAGATCACCATACGGTTTTGGAGGAGGCAGCGCCGGTGGTGGTGCCTCCACTCGCCCCGGCTGGAAATCCGGTGACTGGATATGCAACAGGTTTCATGTCAATCATTTCTTTCATTATACCTTGCTACTTCGTTCTAATTTGGAACTTATAATATACACTAGTATATACTTACTCCGTCTCATTATAAGTGTCATGTTAATGTGATACTTCTTATAAATATTGTGGTTGGACCTAACAAAATCTCATAAAAATGGTTTGTAAGTTTCACTTATAAACATGATCATCTCTCATCAAATATGAAACTTTTAACGTGACACTATTAATGCGACCCTTATAATGAGACAAATGAAACTCCTACTGACATTACCGAGAATTGAAATGTGTAGGTCTGGGTGTAACGAGCATAACTTCGCCAATAGAATGGAATGCTACCGATGCAACGGTCCAAGGGAATCTAGTACTGGAAGATCTTCCTATTTATCGTGA

Protein sequence

>MS.gene014815.t1

MSRPGDWNCRTCNHLNFQRRESCQRCGESRMISGGGGAVDFGGSFLGGRGSSSPFPFTTGPDVRPGDWYCTVGNCGAHNFASRSSCFKCGAPKDIDTFSSDSSDMPRLLRSPYGFGGGSAGGGASTRPGWKSGDWICNRSGCNEHNFANRMECYRCNGPRESSTGRSSYLS